| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T139 |
0-149 |
Sentence |
denotes |
Our results indicated that the colorimetric RT-LAMP assay enabled robust identification of positive samples after a 25- to 30-min incubation at 65°C. |
| T140 |
150-283 |
Sentence |
denotes |
Validation of positive results, however, required confirmation that the RT-LAMP reaction led to the amplification of viral sequences. |
| T141 |
284-420 |
Sentence |
denotes |
To analyze the sequences of many RT-LAMP reaction products, we established multiplexed sequencing of RT-LAMP products (LAMP-sequencing). |
| T142 |
421-504 |
Sentence |
denotes |
LAMP-sequencing is based on Tn5 transposase tagmentation (17) and sample barcoding. |
| T143 |
505-626 |
Sentence |
denotes |
Tagmentation enables fragmentation and direct adapter ligation of DNA samples for analysis by next-generation sequencing. |
| T144 |
627-745 |
Sentence |
denotes |
We used a set of 96 barcoded adapters for tagmentation to barcode the RT-LAMP reaction products in each 96-well plate. |
| T145 |
746-882 |
Sentence |
denotes |
After tagmentation, all barcoded fragments from each plate were pooled and size-selected by bead purification to remove excess adapters. |
| T146 |
883-994 |
Sentence |
denotes |
A second set of barcoded primers, one per plate-pool, was then used to amplify the tagmented RT-LAMP fragments. |
| T147 |
995-1171 |
Sentence |
denotes |
Last, all amplified pools were combined for analysis using one next-generation sequencing run where the origin of each DNA fragment was specified by the two barcodes (Fig. 4A). |
