Our results indicated that the colorimetric RT-LAMP assay enabled robust identification of positive samples after a 25- to 30-min incubation at 65°C. Validation of positive results, however, required confirmation that the RT-LAMP reaction led to the amplification of viral sequences. To analyze the sequences of many RT-LAMP reaction products, we established multiplexed sequencing of RT-LAMP products (LAMP-sequencing). LAMP-sequencing is based on Tn5 transposase tagmentation (17) and sample barcoding. Tagmentation enables fragmentation and direct adapter ligation of DNA samples for analysis by next-generation sequencing. We used a set of 96 barcoded adapters for tagmentation to barcode the RT-LAMP reaction products in each 96-well plate. After tagmentation, all barcoded fragments from each plate were pooled and size-selected by bead purification to remove excess adapters. A second set of barcoded primers, one per plate-pool, was then used to amplify the tagmented RT-LAMP fragments. Last, all amplified pools were combined for analysis using one next-generation sequencing run where the origin of each DNA fragment was specified by the two barcodes (Fig. 4A).