PMC:7045880 / 9174-10111
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7045880","sourcedb":"PMC","sourceid":"7045880","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7045880","text":"After inoculating cells with the nasopharyngeal and oropharyngeal samples, RNA was extracted from the virus-replicated cell culture medium. The RNA was amplified with primers for full-length gene analysis, and NGS was performed using Miseq. De novo assembly of the NGS sequence secured 28,818 bp of the full-length gene. The acquired gene was compared with 57 human and animal coronaviruses, including Wuhan/IVDC-HB-01/2019(GISAID accession ID: EPI_ISL_402119~121), which was first reported in Wuhan, 54 reported full-length SARS-CoV-2 genes, Bat-SARS-like CoV, and human SARS-CoV (Figure 3). The analysis showed that the sequence was included in the same cluster as the previously reported SARS-CoV-2 sequence and showed high homology of \u003e 99.5% with other isolated SARS-CoV-2 sequences. 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