PMC:7045880 / 9174-10111 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"183","span":{"begin":360,"end":365},"obj":"Species"},{"id":"184","span":{"begin":377,"end":390},"obj":"Species"},{"id":"185","span":{"begin":525,"end":535},"obj":"Species"},{"id":"186","span":{"begin":557,"end":560},"obj":"Species"},{"id":"187","span":{"begin":566,"end":571},"obj":"Species"},{"id":"188","span":{"begin":572,"end":580},"obj":"Species"},{"id":"189","span":{"begin":691,"end":701},"obj":"Species"},{"id":"190","span":{"begin":767,"end":777},"obj":"Species"},{"id":"191","span":{"begin":809,"end":816},"obj":"Species"},{"id":"192","span":{"begin":408,"end":443},"obj":"Disease"},{"id":"193","span":{"begin":900,"end":919},"obj":"Disease"}],"attributes":[{"id":"A183","pred":"tao:has_database_id","subj":"183","obj":"Tax:9606"},{"id":"A184","pred":"tao:has_database_id","subj":"184","obj":"Tax:11118"},{"id":"A185","pred":"tao:has_database_id","subj":"185","obj":"Tax:2697049"},{"id":"A186","pred":"tao:has_database_id","subj":"186","obj":"Tax:11118"},{"id":"A187","pred":"tao:has_database_id","subj":"187","obj":"Tax:9606"},{"id":"A188","pred":"tao:has_database_id","subj":"188","obj":"Tax:694009"},{"id":"A189","pred":"tao:has_database_id","subj":"189","obj":"Tax:2697049"},{"id":"A190","pred":"tao:has_database_id","subj":"190","obj":"Tax:2697049"},{"id":"A191","pred":"tao:has_database_id","subj":"191","obj":"Tax:694002"},{"id":"A192","pred":"tao:has_database_id","subj":"192","obj":"MESH:C000657245"},{"id":"A193","pred":"tao:has_database_id","subj":"193","obj":"MESH:C537985"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"After inoculating cells with the nasopharyngeal and oropharyngeal samples, RNA was extracted from the virus-replicated cell culture medium. The RNA was amplified with primers for full-length gene analysis, and NGS was performed using Miseq. De novo assembly of the NGS sequence secured 28,818 bp of the full-length gene. The acquired gene was compared with 57 human and animal coronaviruses, including Wuhan/IVDC-HB-01/2019(GISAID accession ID: EPI_ISL_402119~121), which was first reported in Wuhan, 54 reported full-length SARS-CoV-2 genes, Bat-SARS-like CoV, and human SARS-CoV (Figure 3). The analysis showed that the sequence was included in the same cluster as the previously reported SARS-CoV-2 sequence and showed high homology of \u003e 99.5% with other isolated SARS-CoV-2 sequences. The virus was named BetaCoV/Korea/KCDC03/2020, and its full-length gene sequence was registered in WHO GISAID (GISAID accession ID: EPI_ISL_407193)."}

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T46","span":{"begin":18,"end":23},"obj":"Body_part"},{"id":"T47","span":{"begin":75,"end":78},"obj":"Body_part"},{"id":"T48","span":{"begin":119,"end":123},"obj":"Body_part"},{"id":"T49","span":{"begin":144,"end":147},"obj":"Body_part"},{"id":"T50","span":{"begin":191,"end":195},"obj":"Body_part"},{"id":"T51","span":{"begin":315,"end":319},"obj":"Body_part"},{"id":"T52","span":{"begin":334,"end":338},"obj":"Body_part"},{"id":"T53","span":{"begin":856,"end":860},"obj":"Body_part"}],"attributes":[{"id":"A46","pred":"fma_id","subj":"T46","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A47","pred":"fma_id","subj":"T47","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A48","pred":"fma_id","subj":"T48","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A49","pred":"fma_id","subj":"T49","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A50","pred":"fma_id","subj":"T50","obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"A51","pred":"fma_id","subj":"T51","obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"A52","pred":"fma_id","subj":"T52","obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"A53","pred":"fma_id","subj":"T53","obj":"http://purl.org/sig/ont/fma/fma74402"}],"text":"After inoculating cells with the nasopharyngeal and oropharyngeal samples, RNA was extracted from the virus-replicated cell culture medium. The RNA was amplified with primers for full-length gene analysis, and NGS was performed using Miseq. De novo assembly of the NGS sequence secured 28,818 bp of the full-length gene. The acquired gene was compared with 57 human and animal coronaviruses, including Wuhan/IVDC-HB-01/2019(GISAID accession ID: EPI_ISL_402119~121), which was first reported in Wuhan, 54 reported full-length SARS-CoV-2 genes, Bat-SARS-like CoV, and human SARS-CoV (Figure 3). The analysis showed that the sequence was included in the same cluster as the previously reported SARS-CoV-2 sequence and showed high homology of \u003e 99.5% with other isolated SARS-CoV-2 sequences. The virus was named BetaCoV/Korea/KCDC03/2020, and its full-length gene sequence was registered in WHO GISAID (GISAID accession ID: EPI_ISL_407193)."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T31","span":{"begin":525,"end":533},"obj":"Disease"},{"id":"T32","span":{"begin":547,"end":551},"obj":"Disease"},{"id":"T33","span":{"begin":572,"end":580},"obj":"Disease"},{"id":"T34","span":{"begin":691,"end":699},"obj":"Disease"},{"id":"T35","span":{"begin":767,"end":775},"obj":"Disease"}],"attributes":[{"id":"A31","pred":"mondo_id","subj":"T31","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A32","pred":"mondo_id","subj":"T32","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A33","pred":"mondo_id","subj":"T33","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A34","pred":"mondo_id","subj":"T34","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A35","pred":"mondo_id","subj":"T35","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"}],"text":"After inoculating cells with the nasopharyngeal and oropharyngeal samples, RNA was extracted from the virus-replicated cell culture medium. The RNA was amplified with primers for full-length gene analysis, and NGS was performed using Miseq. De novo assembly of the NGS sequence secured 28,818 bp of the full-length gene. The acquired gene was compared with 57 human and animal coronaviruses, including Wuhan/IVDC-HB-01/2019(GISAID accession ID: EPI_ISL_402119~121), which was first reported in Wuhan, 54 reported full-length SARS-CoV-2 genes, Bat-SARS-like CoV, and human SARS-CoV (Figure 3). The analysis showed that the sequence was included in the same cluster as the previously reported SARS-CoV-2 sequence and showed high homology of \u003e 99.5% with other isolated SARS-CoV-2 sequences. The virus was named BetaCoV/Korea/KCDC03/2020, and its full-length gene sequence was registered in WHO GISAID (GISAID accession ID: EPI_ISL_407193)."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T91","span":{"begin":18,"end":23},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T92","span":{"begin":102,"end":107},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"},{"id":"T93","span":{"begin":119,"end":123},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T94","span":{"begin":191,"end":195},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T95","span":{"begin":315,"end":319},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T96","span":{"begin":334,"end":338},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T97","span":{"begin":360,"end":365},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9606"},{"id":"T98","span":{"begin":370,"end":376},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_33208"},{"id":"T99","span":{"begin":413,"end":415},"obj":"http://purl.obolibrary.org/obo/CLO_0003622"},{"id":"T100","span":{"begin":460,"end":463},"obj":"http://purl.obolibrary.org/obo/CLO_0001053"},{"id":"T101","span":{"begin":536,"end":541},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T102","span":{"begin":566,"end":571},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9606"},{"id":"T103","span":{"begin":793,"end":798},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"},{"id":"T104","span":{"begin":856,"end":860},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"}],"text":"After inoculating cells with the nasopharyngeal and oropharyngeal samples, RNA was extracted from the virus-replicated cell culture medium. The RNA was amplified with primers for full-length gene analysis, and NGS was performed using Miseq. De novo assembly of the NGS sequence secured 28,818 bp of the full-length gene. The acquired gene was compared with 57 human and animal coronaviruses, including Wuhan/IVDC-HB-01/2019(GISAID accession ID: EPI_ISL_402119~121), which was first reported in Wuhan, 54 reported full-length SARS-CoV-2 genes, Bat-SARS-like CoV, and human SARS-CoV (Figure 3). The analysis showed that the sequence was included in the same cluster as the previously reported SARS-CoV-2 sequence and showed high homology of \u003e 99.5% with other isolated SARS-CoV-2 sequences. The virus was named BetaCoV/Korea/KCDC03/2020, and its full-length gene sequence was registered in WHO GISAID (GISAID accession ID: EPI_ISL_407193)."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T59","span":{"begin":441,"end":443},"obj":"Chemical"},{"id":"T60","span":{"begin":917,"end":919},"obj":"Chemical"}],"attributes":[{"id":"A59","pred":"chebi_id","subj":"T59","obj":"http://purl.obolibrary.org/obo/CHEBI_141439"},{"id":"A60","pred":"chebi_id","subj":"T60","obj":"http://purl.obolibrary.org/obo/CHEBI_141439"}],"text":"After inoculating cells with the nasopharyngeal and oropharyngeal samples, RNA was extracted from the virus-replicated cell culture medium. The RNA was amplified with primers for full-length gene analysis, and NGS was performed using Miseq. De novo assembly of the NGS sequence secured 28,818 bp of the full-length gene. The acquired gene was compared with 57 human and animal coronaviruses, including Wuhan/IVDC-HB-01/2019(GISAID accession ID: EPI_ISL_402119~121), which was first reported in Wuhan, 54 reported full-length SARS-CoV-2 genes, Bat-SARS-like CoV, and human SARS-CoV (Figure 3). The analysis showed that the sequence was included in the same cluster as the previously reported SARS-CoV-2 sequence and showed high homology of \u003e 99.5% with other isolated SARS-CoV-2 sequences. The virus was named BetaCoV/Korea/KCDC03/2020, and its full-length gene sequence was registered in WHO GISAID (GISAID accession ID: EPI_ISL_407193)."}

{"project":"LitCovid-sentences","denotations":[{"id":"T88","span":{"begin":0,"end":139},"obj":"Sentence"},{"id":"T89","span":{"begin":140,"end":240},"obj":"Sentence"},{"id":"T90","span":{"begin":241,"end":320},"obj":"Sentence"},{"id":"T91","span":{"begin":321,"end":592},"obj":"Sentence"},{"id":"T92","span":{"begin":593,"end":788},"obj":"Sentence"},{"id":"T93","span":{"begin":789,"end":937},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"After inoculating cells with the nasopharyngeal and oropharyngeal samples, RNA was extracted from the virus-replicated cell culture medium. The RNA was amplified with primers for full-length gene analysis, and NGS was performed using Miseq. De novo assembly of the NGS sequence secured 28,818 bp of the full-length gene. The acquired gene was compared with 57 human and animal coronaviruses, including Wuhan/IVDC-HB-01/2019(GISAID accession ID: EPI_ISL_402119~121), which was first reported in Wuhan, 54 reported full-length SARS-CoV-2 genes, Bat-SARS-like CoV, and human SARS-CoV (Figure 3). The analysis showed that the sequence was included in the same cluster as the previously reported SARS-CoV-2 sequence and showed high homology of \u003e 99.5% with other isolated SARS-CoV-2 sequences. The virus was named BetaCoV/Korea/KCDC03/2020, and its full-length gene sequence was registered in WHO GISAID (GISAID accession ID: EPI_ISL_407193)."}