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PubMed:8394350 JSONTXT 18 Projects

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Id Subject Object Predicate Lexical cue
T1 0-113 Sentence denotes Expression, purification, crystallization, and biochemical characterization of a recombinant protein phosphatase.
T1 0-113 Sentence denotes Expression, purification, crystallization, and biochemical characterization of a recombinant protein phosphatase.
T2 114-212 Sentence denotes A protein phosphatase (PPase) from the bacteriophage lambda was overexpressed in Escherichia coli.
T2 114-212 Sentence denotes A protein phosphatase (PPase) from the bacteriophage lambda was overexpressed in Escherichia coli.
T3 213-344 Sentence denotes The recombinant enzyme was purified to homogeneity yielding approximately 17 mg of enzyme from a single liter of bacterial culture.
T3 213-344 Sentence denotes The recombinant enzyme was purified to homogeneity yielding approximately 17 mg of enzyme from a single liter of bacterial culture.
T4 345-441 Sentence denotes Biochemical characterization of the enzyme showed that it required Mn2+ or Ni2+ as an activator.
T4 345-441 Sentence denotes Biochemical characterization of the enzyme showed that it required Mn2+ or Ni2+ as an activator.
T5 442-551 Sentence denotes The recombinant enzyme was active toward serine, threonine, and tyrosine phosphoproteins and phosphopeptides.
T5 442-551 Sentence denotes The recombinant enzyme was active toward serine, threonine, and tyrosine phosphoproteins and phosphopeptides.
T6 552-657 Sentence denotes Surprisingly, the bacterial histidyl phosphoprotein, NRII, was also dephosphorylated by the lambda-PPase.
T6 552-657 Sentence denotes Surprisingly, the bacterial histidyl phosphoprotein, NRII, was also dephosphorylated by the lambda-PPase.
T7 658-861 Sentence denotes The lambda-PPase shares a number of kinetic and structural properties with the eukaryotic Ser/Thr phosphatases, suggesting that the lambda-PPase will serve as a good model for structure-function studies.
T7 658-861 Sentence denotes The lambda-PPase shares a number of kinetic and structural properties with the eukaryotic Ser/Thr phosphatases, suggesting that the lambda-PPase will serve as a good model for structure-function studies.
T8 862-947 Sentence denotes Crystallization of the recombinant purified lambda-PPase yielded monoclinic crystals.
T8 862-947 Sentence denotes Crystallization of the recombinant purified lambda-PPase yielded monoclinic crystals.
T9 948-1023 Sentence denotes The crystals diffract to 4.0 A when exposed to synchrotron x-ray radiation.
T9 948-1023 Sentence denotes The crystals diffract to 4.0 A when exposed to synchrotron x-ray radiation.