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Id Subject Object Predicate Lexical cue
T1 0-63 Sentence denotes Purification and properties of an acidic protein from rat skin.
T1 0-63 Sentence denotes Purification and properties of an acidic protein from rat skin.
T2 64-214 Sentence denotes An acidic protein, extractable in neutral salt solutions from rat skin, was markedly enriched when precipitated by dialysis against 0.5 M acetic acid.
T2 64-214 Sentence denotes An acidic protein, extractable in neutral salt solutions from rat skin, was markedly enriched when precipitated by dialysis against 0.5 M acetic acid.
T3 215-447 Sentence denotes After dissolving the precipitate in 0.5 M Tris-HCl buffer, pH 8.0, the protein was disaggregated by the addition of the nonionic detergent Triton X-100 and purified by chromatography on Sephadex G-100 and DEAE-Sephadex A-50 columns.
T3 215-447 Sentence denotes After dissolving the precipitate in 0.5 M Tris-HCl buffer, pH 8.0, the protein was disaggregated by the addition of the nonionic detergent Triton X-100 and purified by chromatography on Sephadex G-100 and DEAE-Sephadex A-50 columns.
T4 448-854 Sentence denotes The protein isolated under nondenaturing conditions appeared to be essentially homogeneous by its migration as a single band on (a) cellulose acetate membrane electrophoresis at pH 8.6; (B) 4% and 7.5% polyacrylamide gel electrophoresis at ph 8.9; (C) sodium dodecyl sulfate (10%) polyacrylamide gel electrophoresis at pH 7.0; and by (d) its complete freedom from collagen, the major contaminating protein.
T4 448-854 Sentence denotes The protein isolated under nondenaturing conditions appeared to be essentially homogeneous by its migration as a single band on (a) cellulose acetate membrane electrophoresis at pH 8.6; (B) 4% and 7.5% polyacrylamide gel electrophoresis at ph 8.9; (C) sodium dodecyl sulfate (10%) polyacrylamide gel electrophoresis at pH 7.0; and by (d) its complete freedom from collagen, the major contaminating protein.
T5 855-1067 Sentence denotes The molecular weight of the protein was determined as 76,000 +/- 2,000 from its electrophoretic mobility in sodium dodecyl sulfate polyacrylamide gels and 75,000 from its elution volume in Sephadex G-100 columns.
T5 855-1067 Sentence denotes The molecular weight of the protein was determined as 76,000 +/- 2,000 from its electrophoretic mobility in sodium dodecyl sulfate polyacrylamide gels and 75,000 from its elution volume in Sephadex G-100 columns.
T6 1068-1144 Sentence denotes Reduction and alkylation of the protein failed to generate smaller subunits.
T6 1068-1144 Sentence denotes Reduction and alkylation of the protein failed to generate smaller subunits.
T7 1145-1305 Sentence denotes The amino acid composition of the protein showed that it was relatively rich in glutamic and aspartic acids, which together comprised 25% of its total residues.
T7 1145-1305 Sentence denotes The amino acid composition of the protein showed that it was relatively rich in glutamic and aspartic acids, which together comprised 25% of its total residues.
T8 1306-1482 Sentence denotes Hydrophobic amino acids like phenylalanine, leucine, isoleucine, valine, methionine, alanine, proline, and cystine accounted for about 34% of the total residues in the protein.
T8 1306-1482 Sentence denotes Hydrophobic amino acids like phenylalanine, leucine, isoleucine, valine, methionine, alanine, proline, and cystine accounted for about 34% of the total residues in the protein.
T9 1483-1583 Sentence denotes No free NH2-terminal amino acid could be detected in the purified protein by the dansylation method.
T9 1483-1583 Sentence denotes No free NH2-terminal amino acid could be detected in the purified protein by the dansylation method.
T10 1584-1635 Sentence denotes Each mole of protein contained 11 mol of phosphate.
T10 1584-1635 Sentence denotes Each mole of protein contained 11 mol of phosphate.
T11 1636-1729 Sentence denotes Triton X-100 was necessary for achieving nondestructive disaggregation of the acidic protein.
T11 1636-1729 Sentence denotes Triton X-100 was necessary for achieving nondestructive disaggregation of the acidic protein.
T12 1730-1811 Sentence denotes Each mole of protein bound about 3200 mol of Triton X-100 or 10 mol of Congo red.
T12 1730-1811 Sentence denotes Each mole of protein bound about 3200 mol of Triton X-100 or 10 mol of Congo red.
T13 1812-1922 Sentence denotes While the detergent binding could be reversed by dialysis, Congo red formed a stable complex with the protein.
T13 1812-1922 Sentence denotes While the detergent binding could be reversed by dialysis, Congo red formed a stable complex with the protein.