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PMC:3317373 / 11660-15415 JSONTXT 26 Projects

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Id Subject Object Predicate Lexical cue
T122 0-2 Sentence denotes 3.
T123 3-10 Sentence denotes Results
T4844 12-16 Sentence denotes 3.1.
T124 12-16 Sentence denotes 3.1.
T4845 17-95 Sentence denotes Depletion of Nrf2 Aggravated the Cell Death in Astrocytes after Scratch Injury
T125 17-95 Sentence denotes Depletion of Nrf2 Aggravated the Cell Death in Astrocytes after Scratch Injury
T4846 96-175 Sentence denotes Cell death was detected by microscope and CCK-8 analysis at 24 h after scratch.
T126 96-175 Sentence denotes Cell death was detected by microscope and CCK-8 analysis at 24 h after scratch.
T4847 176-324 Sentence denotes The detachment from culture plate, cell lost, and cytoplasmic process distortion were mostly observed in both sides of scratch line in group KO TBI.
T127 176-324 Sentence denotes The detachment from culture plate, cell lost, and cytoplasmic process distortion were mostly observed in both sides of scratch line in group KO TBI.
T4848 325-512 Sentence denotes CCK-8 assay also showed lower OD value of group KO TBI (0.38 ± 0.064) than that of group WT TBI (0.98 ± 0.098) (P < 0.01), which also suggested more cell death in group KO TBI (Figure 1).
T128 325-512 Sentence denotes CCK-8 assay also showed lower OD value of group KO TBI (0.38 ± 0.064) than that of group WT TBI (0.98 ± 0.098) (P < 0.01), which also suggested more cell death in group KO TBI (Figure 1).
T4849 513-570 Sentence denotes There was no difference between two sham-operated groups.
T129 513-570 Sentence denotes There was no difference between two sham-operated groups.
T5106 572-576 Sentence denotes 3.2.
T130 572-576 Sentence denotes 3.2.
T5107 577-668 Sentence denotes Disruption of Nrf2 Enhanced Upregulation of NF-κB DNA-Binding Activity after Scratch Injury
T131 577-668 Sentence denotes Disruption of Nrf2 Enhanced Upregulation of NF-κB DNA-Binding Activity after Scratch Injury
T5108 669-737 Sentence denotes It has been reported that NF-κB is activated in brain after TBI [2].
T132 669-737 Sentence denotes It has been reported that NF-κB is activated in brain after TBI [2].
T5109 738-864 Sentence denotes Here, we studied the DNA-binding activity of NF-κB of astrocytes from Nrf2 WT or KO mice by EMSA at 24 h after scratch injury.
T133 738-864 Sentence denotes Here, we studied the DNA-binding activity of NF-κB of astrocytes from Nrf2 WT or KO mice by EMSA at 24 h after scratch injury.
T5110 865-1075 Sentence denotes Scratch injury induced activation of NF-κB in astrocytes of both genotypes, while higher NF-κB activity was observed in group KO TBI than in group WT TBI (2.67 ± 0.173 versus 2.28 ± 0.072, P < 0.01) (Figure 2).
T134 865-1075 Sentence denotes Scratch injury induced activation of NF-κB in astrocytes of both genotypes, while higher NF-κB activity was observed in group KO TBI than in group WT TBI (2.67 ± 0.173 versus 2.28 ± 0.072, P < 0.01) (Figure 2).
T5417 1077-1081 Sentence denotes 3.3.
T135 1077-1081 Sentence denotes 3.3.
T5418 1082-1183 Sentence denotes Expression of Proinflammatory Cytokines Was Elevated in Nrf2 Knockout Astrocytes after Scratch Injury
T136 1082-1183 Sentence denotes Expression of Proinflammatory Cytokines Was Elevated in Nrf2 Knockout Astrocytes after Scratch Injury
T5419 1184-1291 Sentence denotes As we all know, TNF-α, IL-1β, and IL-6 are regulated by NF-κB and reflect the endogenous activity of NF-κB.
T137 1184-1291 Sentence denotes As we all know, TNF-α, IL-1β, and IL-6 are regulated by NF-κB and reflect the endogenous activity of NF-κB.
T5420 1292-1392 Sentence denotes It has been revealed that these proinflammatory cytokines are increased after TBI in animals [2, 8].
T138 1292-1392 Sentence denotes It has been revealed that these proinflammatory cytokines are increased after TBI in animals [2, 8].
T5421 1393-1544 Sentence denotes And our previous study has proved that higher levels of such proinflammatory cytokines were observed in the brain of Nrf2-deficient mice after TBI [5].
T139 1393-1544 Sentence denotes And our previous study has proved that higher levels of such proinflammatory cytokines were observed in the brain of Nrf2-deficient mice after TBI [5].
T5422 1545-1651 Sentence denotes But it is still ambiguous about the relationship between these cytokines and Nrf2 in astrocytes after TBI.
T140 1545-1651 Sentence denotes But it is still ambiguous about the relationship between these cytokines and Nrf2 in astrocytes after TBI.
T5423 1652-1777 Sentence denotes Here, we tested the levels of TNF-α, IL-1β, and IL-6 in cultured astrocytes at 24 h after scratch injury by RT-PCR and ELISA.
T141 1652-1777 Sentence denotes Here, we tested the levels of TNF-α, IL-1β, and IL-6 in cultured astrocytes at 24 h after scratch injury by RT-PCR and ELISA.
T5424 1778-1953 Sentence denotes It was shown that the mRNA levels of TNF-α, IL-1β, and IL-6 were increased after scratch in both WT and KO astrocytes when compared with their sham counterparts, respectively.
T142 1778-1953 Sentence denotes It was shown that the mRNA levels of TNF-α, IL-1β, and IL-6 were increased after scratch in both WT and KO astrocytes when compared with their sham counterparts, respectively.
T5425 1954-2194 Sentence denotes Moreover, the mRNA levels of these cytokines in group KO TBI were much higher than those in group WT TBI (1.66 ± 0.085 versus 1.39 ± 0.110 for TNF-α, 1.34 ± 0.064 versus 0.73 ± 0.088 for IL-1β, and 1.28 ± 0.102 versus 0.99 ± 0.073 for IL-6.
T143 1954-2194 Sentence denotes Moreover, the mRNA levels of these cytokines in group KO TBI were much higher than those in group WT TBI (1.66 ± 0.085 versus 1.39 ± 0.110 for TNF-α, 1.34 ± 0.064 versus 0.73 ± 0.088 for IL-1β, and 1.28 ± 0.102 versus 0.99 ± 0.073 for IL-6.
T5426 2195-2225 Sentence denotes P < 0.01) (Figures 3(a)–3(d)).
T144 2195-2225 Sentence denotes P < 0.01) (Figures 3(a)–3(d)).
T5427 2226-2392 Sentence denotes The results of ELISA revealed that the protein level of TNF-α was upregulated after scratch injury in WT and KO astrocytes when compared with their sham counterparts.
T145 2226-2392 Sentence denotes The results of ELISA revealed that the protein level of TNF-α was upregulated after scratch injury in WT and KO astrocytes when compared with their sham counterparts.
T5428 2393-2512 Sentence denotes And it was also higher in group KO TBI than in group WT TBI (3.67 ± 0.156 versus 2.31 ± 0.087, P < 0.01) (Figure 4(a)).
T146 2393-2512 Sentence denotes And it was also higher in group KO TBI than in group WT TBI (3.67 ± 0.156 versus 2.31 ± 0.087, P < 0.01) (Figure 4(a)).
T5429 2513-2578 Sentence denotes Similar tendency was observed in protein level of IL-6 and IL-1β.
T147 2513-2578 Sentence denotes Similar tendency was observed in protein level of IL-6 and IL-1β.
T5430 2579-2689 Sentence denotes For IL-6, it was 36.07 ± 0.786 for group KO TBI while 25.76 ± 0.536 for group WT TBI (P < 0.01) (Figure 4(b)).
T148 2579-2689 Sentence denotes For IL-6, it was 36.07 ± 0.786 for group KO TBI while 25.76 ± 0.536 for group WT TBI (P < 0.01) (Figure 4(b)).
T5431 2690-2803 Sentence denotes For IL-1β, it was 190.75 ± 6.339 for group KO TBI while 154.50 ± 5.348 for group WT TBI (P < 0.01) (Figure 4(c)).
T149 2690-2803 Sentence denotes For IL-1β, it was 190.75 ± 6.339 for group KO TBI while 154.50 ± 5.348 for group WT TBI (P < 0.01) (Figure 4(c)).
T6473 2805-2809 Sentence denotes 3.4.
T150 2805-2809 Sentence denotes 3.4.
T6474 2810-2912 Sentence denotes Expression and Activity of MMP9 Were Greatly Enhanced in Nrf2 Knockout Astrocytes after Scratch Injury
T151 2810-2912 Sentence denotes Expression and Activity of MMP9 Were Greatly Enhanced in Nrf2 Knockout Astrocytes after Scratch Injury
T6475 2913-2993 Sentence denotes MMP9 is an important gelatinase to induce or aggravate the inflammation process.
T152 2913-2993 Sentence denotes MMP9 is an important gelatinase to induce or aggravate the inflammation process.
T6476 2994-3167 Sentence denotes In the present study, we elevated the mRNA, protein levels, and activity of MMP9 in astrocytes at 24 h after scratch injury by RT-PCR, western blot, and gelatine zymography.
T153 2994-3167 Sentence denotes In the present study, we elevated the mRNA, protein levels, and activity of MMP9 in astrocytes at 24 h after scratch injury by RT-PCR, western blot, and gelatine zymography.
T6477 3168-3286 Sentence denotes Expression of MMP9 was elevated after scratch injury in WT and KO astrocytes as compared with their sham counterparts.
T154 3168-3286 Sentence denotes Expression of MMP9 was elevated after scratch injury in WT and KO astrocytes as compared with their sham counterparts.
T6478 3287-3427 Sentence denotes The mRNA level of MMP9 was higher in group KO TBI than in group WT TBI (1.36 ± 0.090 versus 1.01 ± 0.068, P < 0.01) (Figures 3(a) and 3(e)).
T155 3287-3427 Sentence denotes The mRNA level of MMP9 was higher in group KO TBI than in group WT TBI (1.36 ± 0.090 versus 1.01 ± 0.068, P < 0.01) (Figures 3(a) and 3(e)).
T6479 3428-3599 Sentence denotes Significant difference was also discovered in MMP9 protein level by western blot, as 1.44 ± 0.076 for group KO TBI and 1.13 ± 0.048 for group WT TBI (P < 0.01) (Figure 5).
T156 3428-3599 Sentence denotes Significant difference was also discovered in MMP9 protein level by western blot, as 1.44 ± 0.076 for group KO TBI and 1.13 ± 0.048 for group WT TBI (P < 0.01) (Figure 5).
T6480 3600-3755 Sentence denotes Gelatine zymography revealed higher MMP9 activity in group KO TBI than that in group WT TBI too (101.76 ± 6.343 versus 76.32 ± 3.388, P < 0.01) (Figure 6).
T157 3600-3755 Sentence denotes Gelatine zymography revealed higher MMP9 activity in group KO TBI than that in group WT TBI too (101.76 ± 6.343 versus 76.32 ± 3.388, P < 0.01) (Figure 6).