Id |
Subject |
Object |
Predicate |
Lexical cue |
T5849 |
0-145 |
Sentence |
denotes |
Fluoro-Jade B (FJB) staining was used to identify degenerating neurons in tissues obtained from non-SE and 3 days post-SE animals in every group. |
T100 |
0-145 |
Sentence |
denotes |
Fluoro-Jade B (FJB) staining was used to identify degenerating neurons in tissues obtained from non-SE and 3 days post-SE animals in every group. |
T5850 |
146-248 |
Sentence |
denotes |
In our previous [18,25] and preliminary data, neuronal damage was first detectable at 3 days after SE. |
T101 |
146-248 |
Sentence |
denotes |
In our previous [18,25] and preliminary data, neuronal damage was first detectable at 3 days after SE. |
T5851 |
249-325 |
Sentence |
denotes |
Therefore, we determined 3 days after SE as the best time point to look FJB. |
T102 |
249-325 |
Sentence |
denotes |
Therefore, we determined 3 days after SE as the best time point to look FJB. |
T5852 |
326-448 |
Sentence |
denotes |
Briefly, sections were rinsed in distilled water, and mounted onto gelatin-coated slides and then dried on a slide warmer. |
T103 |
326-448 |
Sentence |
denotes |
Briefly, sections were rinsed in distilled water, and mounted onto gelatin-coated slides and then dried on a slide warmer. |
T5853 |
449-565 |
Sentence |
denotes |
The slides were immersed in 100% ethanol for 3 min, followed by 70% ethanol for 2 min and distilled water for 2 min. |
T104 |
449-565 |
Sentence |
denotes |
The slides were immersed in 100% ethanol for 3 min, followed by 70% ethanol for 2 min and distilled water for 2 min. |
T5854 |
566-662 |
Sentence |
denotes |
The slides were then transferred to 0.06% potassium permanganate for 15 min and gently agitated. |
T105 |
566-662 |
Sentence |
denotes |
The slides were then transferred to 0.06% potassium permanganate for 15 min and gently agitated. |
T5855 |
663-918 |
Sentence |
denotes |
After rinsing in distilled water for 2 min, the slides were incubated for 30 min in 0.001% FJB (Histo-Chem Inc., Jefferson, AR), freshly prepared by adding 20 ml of a 0.01% stock FJB solution to 180 ml of 0.1% acetic acid, with gentle shaking in the dark. |
T106 |
663-918 |
Sentence |
denotes |
After rinsing in distilled water for 2 min, the slides were incubated for 30 min in 0.001% FJB (Histo-Chem Inc., Jefferson, AR), freshly prepared by adding 20 ml of a 0.01% stock FJB solution to 180 ml of 0.1% acetic acid, with gentle shaking in the dark. |
T5856 |
919-1093 |
Sentence |
denotes |
After rinsing for 1 min in each of three changes of distilled water, the slides were dried, dehydrated in xylene and coverslipped with DPX (Sigma-Aldrich Co., St. Louis, MO). |
T107 |
919-1093 |
Sentence |
denotes |
After rinsing for 1 min in each of three changes of distilled water, the slides were dried, dehydrated in xylene and coverslipped with DPX (Sigma-Aldrich Co., St. Louis, MO). |
T5857 |
1094-1199 |
Sentence |
denotes |
For stereological study, every sixth section in the series throughout the entire PC was used (see below). |
T108 |
1094-1199 |
Sentence |
denotes |
For stereological study, every sixth section in the series throughout the entire PC was used (see below). |