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PMC:3279418 / 29511-30212 JSONTXT 14 Projects

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Id Subject Object Predicate Lexical cue
T10960 0-32 Sentence denotes BMDC-T cell in vitro interaction
T204 0-32 Sentence denotes BMDC-T cell in vitro interaction
T10961 33-129 Sentence denotes WT and cpdm BMDC (5×104) were stimulated with medium, 1 µg/mL LPS, 25 µg/mL and 5 µg/mL Pam3CYS.
T205 33-129 Sentence denotes WT and cpdm BMDC (5×104) were stimulated with medium, 1 µg/mL LPS, 25 µg/mL and 5 µg/mL Pam3CYS.
T10962 130-187 Sentence denotes 24 hours later, cells were collected and washed with PBS.
T206 130-187 Sentence denotes 24 hours later, cells were collected and washed with PBS.
T10963 188-366 Sentence denotes Allogeneic naïve CD4+ T cells were isolated from spleens of BALB/c mice by negative selection kit (Invitrogen) and were then added at 2.5×105 and co-cultured with activated BMDC.
T207 188-366 Sentence denotes Allogeneic naïve CD4+ T cells were isolated from spleens of BALB/c mice by negative selection kit (Invitrogen) and were then added at 2.5×105 and co-cultured with activated BMDC.
T10964 367-472 Sentence denotes After 5 days, supernatant was collected and the secretion of IFNγ, IL4, IL2, and IL17A measured by ELISA.
T208 367-472 Sentence denotes After 5 days, supernatant was collected and the secretion of IFNγ, IL4, IL2, and IL17A measured by ELISA.
T10965 473-630 Sentence denotes Negative controls are 1) stimulated BMDC without co-culture with allogeneic CD4+ T cells; 2) allogeneic CD4+ T cells without co-culture with stimulated BMDC.
T209 473-630 Sentence denotes Negative controls are 1) stimulated BMDC without co-culture with allogeneic CD4+ T cells; 2) allogeneic CD4+ T cells without co-culture with stimulated BMDC.
T10966 631-701 Sentence denotes Both negative controls show no production of aforementioned cytokines.
T210 631-701 Sentence denotes Both negative controls show no production of aforementioned cytokines.