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PMC:3279418 / 27163-28081 JSONTXT 12 Projects

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Id Subject Object Predicate Lexical cue
T9960 0-27 Sentence denotes Constructs and transfection
T181 0-27 Sentence denotes Constructs and transfection
T9961 28-135 Sentence denotes The complementary DNA (cDNA) of the mouse Sharpin gene was cloned and amplified from RAW264.7 RNA extracts.
T182 28-135 Sentence denotes The complementary DNA (cDNA) of the mouse Sharpin gene was cloned and amplified from RAW264.7 RNA extracts.
T183 136-265 Sentence denotes The primer sequences were forward, 5′-CC ATG GCG ATG TCG CCG CCC GCC GGC GGT; reverse, 5′- AAG CTT CTA GGT GGA AGC TGC AGC AAG A.
T9962 136-329 Sentence denotes The primer sequences were forward, 5′-CC ATG GCG ATG TCG CCG CCC GCC GGC GGT; reverse, 5′- AAG CTT CTA GGT GGA AGC TGC AGC AAG A. The Sharpin cDNA was cloned into expression vector pFLAG-CMV-2.
T184 266-329 Sentence denotes The Sharpin cDNA was cloned into expression vector pFLAG-CMV-2.
T9963 330-419 Sentence denotes Murine fibroblasts and macrophages were were used to express recombinant SHARPIN protein.
T185 330-419 Sentence denotes Murine fibroblasts and macrophages were were used to express recombinant SHARPIN protein.
T9964 420-500 Sentence denotes Cells (2×104) were seeded in 96-well treated plates the day before transfection.
T186 420-500 Sentence denotes Cells (2×104) were seeded in 96-well treated plates the day before transfection.
T9965 501-607 Sentence denotes After overnight incubation, 200 ng pFLAG-SHARPIN plasmids were transfected with 0.5 ul Lipofectamine 2000.
T187 501-607 Sentence denotes After overnight incubation, 200 ng pFLAG-SHARPIN plasmids were transfected with 0.5 ul Lipofectamine 2000.
T9966 608-671 Sentence denotes 24 hours later, cells were incubated with fresh culture medium.
T188 608-671 Sentence denotes 24 hours later, cells were incubated with fresh culture medium.
T9967 672-782 Sentence denotes After another 24 hours, cells were lysed to confirm the FLAG-SHARPIN expression by immunoblots with anti-FLAG.
T189 672-782 Sentence denotes After another 24 hours, cells were lysed to confirm the FLAG-SHARPIN expression by immunoblots with anti-FLAG.
T9968 783-918 Sentence denotes Cells with no transfection and transfected with empty vector pFLAG-CMV-2 were used as negative control in all transfection experiments.
T190 783-918 Sentence denotes Cells with no transfection and transfected with empty vector pFLAG-CMV-2 were used as negative control in all transfection experiments.