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PMC:2889865 / 5332-16309 JSONTXT 18 Projects

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Id Subject Object Predicate Lexical cue
T37 0-7 Sentence denotes Results
T38 9-48 Sentence denotes Regulation of AP-1 and NF-κB activation
T3051 49-259 Sentence denotes The transcription factors NF-κB and AP-1 play key roles in the initiation of an inflammatory response by inducing the expression and secretion of chemokines and cytokines that attract and activate immune cells.
T39 49-259 Sentence denotes The transcription factors NF-κB and AP-1 play key roles in the initiation of an inflammatory response by inducing the expression and secretion of chemokines and cytokines that attract and activate immune cells.
T3052 260-404 Sentence denotes However, the signal transduction pathways and subsequent inflammatory cytokine induction by these transcription factors is not fully elucidated.
T40 260-404 Sentence denotes However, the signal transduction pathways and subsequent inflammatory cytokine induction by these transcription factors is not fully elucidated.
T3053 405-518 Sentence denotes The present study is aimed at determining the involvement of AP-1 and NF-κB in cytokine induction and regulation.
T41 405-518 Sentence denotes The present study is aimed at determining the involvement of AP-1 and NF-κB in cytokine induction and regulation.
T3054 519-658 Sentence denotes PMA treatment resulted in an up-regulation of AP-1 after 2 h exposure and continued to increase throughout the analysis period (figure 1a).
T42 519-658 Sentence denotes PMA treatment resulted in an up-regulation of AP-1 after 2 h exposure and continued to increase throughout the analysis period (figure 1a).
T3055 659-741 Sentence denotes HK E. coli treatment did not affect AP-1 activation in Jurkat T-cells (figure 1b).
T43 659-741 Sentence denotes HK E. coli treatment did not affect AP-1 activation in Jurkat T-cells (figure 1b).
T3056 742-999 Sentence denotes To determine the involvement of associated pathways, we exposed cells to Ca2+ ionophore with or without PMA and observed a modest involvement of Ca2+ in PMA-dependent AP-1 activation (figure 1c) while Ca2+ alone did not alter AP-1 activity (data not shown).
T44 742-999 Sentence denotes To determine the involvement of associated pathways, we exposed cells to Ca2+ ionophore with or without PMA and observed a modest involvement of Ca2+ in PMA-dependent AP-1 activation (figure 1c) while Ca2+ alone did not alter AP-1 activity (data not shown).
T3057 1000-1218 Sentence denotes Furthermore, AP-1 activity decreased in a TCR-deficient Jurkat cell line when exposed to PMA compared to the parent cell line indicating that regulation of AP-1 was only partially T-cell receptor dependent (figure 1d).
T45 1000-1218 Sentence denotes Furthermore, AP-1 activity decreased in a TCR-deficient Jurkat cell line when exposed to PMA compared to the parent cell line indicating that regulation of AP-1 was only partially T-cell receptor dependent (figure 1d).
T46 1219-1299 Sentence denotes Figure 1 AP-1 activation following long-term exposure of Jurkat T-cells to PMA.
T15023 1229-1299 Sentence denotes AP-1 activation following long-term exposure of Jurkat T-cells to PMA.
T15024 1300-1727 Sentence denotes Jurkat T-cells were transfected with luciferase reporter plasmids containing the AP-1 cis-elements. (A) Time-dependent AP-1 activation in response to PMA. (B) HK E. coli does not activate AP-1. (C) Dose-dependent activation of AP-1 were performed using PMA alone (grey bars) and in combination with calcium ionophore (CaI 955 μM, black bars). (D) TCR-/- deficient cells responded to PMA (162 nM) by up-regulating AP-1 activity.
T47 1300-1727 Sentence denotes Jurkat T-cells were transfected with luciferase reporter plasmids containing the AP-1 cis-elements. (A) Time-dependent AP-1 activation in response to PMA. (B) HK E. coli does not activate AP-1. (C) Dose-dependent activation of AP-1 were performed using PMA alone (grey bars) and in combination with calcium ionophore (CaI 955 μM, black bars). (D) TCR-/- deficient cells responded to PMA (162 nM) by up-regulating AP-1 activity.
T15025 1728-1817 Sentence denotes Statistical significance from the control was determined using Student's t-test. (n = 4).
T48 1728-1817 Sentence denotes Statistical significance from the control was determined using Student's t-test. (n = 4).
T15026 1818-1853 Sentence denotes Controls were arbitrarily set to 1.
T49 1818-1853 Sentence denotes Controls were arbitrarily set to 1.
T3058 1856-1940 Sentence denotes NF-κB levels showed a transient increase at 1 min after exposure to PMA (figure 2a).
T50 1856-1940 Sentence denotes NF-κB levels showed a transient increase at 1 min after exposure to PMA (figure 2a).
T3059 1941-2076 Sentence denotes However, 1 h after exposure the NF-κB levels began to drop reaching the lowest levels by 6 h, after which they increased again by 24 h.
T51 1941-2076 Sentence denotes However, 1 h after exposure the NF-κB levels began to drop reaching the lowest levels by 6 h, after which they increased again by 24 h.
T3060 2077-2258 Sentence denotes Exposure of Jurkat T-cells to HK E. coli resulted in a dose-dependent NF-κB activation, with the highest activity observed at a relative concentration of 5 × 107 CFU/ml (figure 2b).
T52 2077-2258 Sentence denotes Exposure of Jurkat T-cells to HK E. coli resulted in a dose-dependent NF-κB activation, with the highest activity observed at a relative concentration of 5 × 107 CFU/ml (figure 2b).
T3061 2259-2468 Sentence denotes The time-dependent activation of NF-κB by HK E. coli was assessed further using the optimal concentration obtained from figure 2b and showed that the NF-κB activity increased after 3 h of exposure (figure 2c).
T53 2259-2468 Sentence denotes The time-dependent activation of NF-κB by HK E. coli was assessed further using the optimal concentration obtained from figure 2b and showed that the NF-κB activity increased after 3 h of exposure (figure 2c).
T3062 2469-2634 Sentence denotes Furthermore, increased intracellular Ca2+ reversed the PMA dependent NF-κB inhibition (figure 2d) and reduced the HK E. coli -dependent NF-κB activation (figure 2e).
T54 2469-2634 Sentence denotes Furthermore, increased intracellular Ca2+ reversed the PMA dependent NF-κB inhibition (figure 2d) and reduced the HK E. coli -dependent NF-κB activation (figure 2e).
T55 2635-2754 Sentence denotes Figure 2 NF-κB down-regulation by PMA and up-regulation by heat killed E. coli MG1655 following long-term stimulation.
T15303 2645-2754 Sentence denotes NF-κB down-regulation by PMA and up-regulation by heat killed E. coli MG1655 following long-term stimulation.
T15304 2755-2925 Sentence denotes Transfection of Jurkat T-cells was performed using luciferase reporter plasmids containing NF-κB cis-elements. (A) Time-dependent stimulation of Jurkat T-cells using PMA.
T56 2755-2925 Sentence denotes Transfection of Jurkat T-cells was performed using luciferase reporter plasmids containing NF-κB cis-elements. (A) Time-dependent stimulation of Jurkat T-cells using PMA.
T15305 2926-3019 Sentence denotes NF-κB activation was evaluated using HK E. coli in a (B) dose- and (C) time-dependant manner.
T57 2926-3019 Sentence denotes NF-κB activation was evaluated using HK E. coli in a (B) dose- and (C) time-dependant manner.
T15306 3020-3170 Sentence denotes Calcium ionophore increased NF-κB activity following PMA exposure (E) and resulted in a negative regulation in response to HK E. coli stimulation (D).
T58 3020-3170 Sentence denotes Calcium ionophore increased NF-κB activity following PMA exposure (E) and resulted in a negative regulation in response to HK E. coli stimulation (D).
T15307 3171-3260 Sentence denotes Statistical significance from the control was determined using Student's t-test. (n = 4).
T59 3171-3260 Sentence denotes Statistical significance from the control was determined using Student's t-test. (n = 4).
T15308 3261-3296 Sentence denotes Controls were arbitrarily set to 1.
T60 3261-3296 Sentence denotes Controls were arbitrarily set to 1.
T3931 3298-3333 Sentence denotes Induction of inflammatory responses
T61 3298-3333 Sentence denotes Induction of inflammatory responses
T3932 3334-3495 Sentence denotes The ability of PMA and HK E. coli to induce an inflammatory response in Jurkat T-cells was evaluated using a multiplex cytokine assay following 24 h stimulation.
T62 3334-3495 Sentence denotes The ability of PMA and HK E. coli to induce an inflammatory response in Jurkat T-cells was evaluated using a multiplex cytokine assay following 24 h stimulation.
T3933 3496-3622 Sentence denotes The cytokine profile revealed an enhanced induction of the pro-inflammatory cytokines IL-2, IL-6, TNF and the chemokine CXCL8.
T63 3496-3622 Sentence denotes The cytokine profile revealed an enhanced induction of the pro-inflammatory cytokines IL-2, IL-6, TNF and the chemokine CXCL8.
T3934 3623-3754 Sentence denotes The levels of the anti-inflammatory cytokine IL-10 were unaffected by PMA but were significantly decreased by HK E. coli (Table 1).
T64 3623-3754 Sentence denotes The levels of the anti-inflammatory cytokine IL-10 were unaffected by PMA but were significantly decreased by HK E. coli (Table 1).
T3935 3755-3858 Sentence denotes These results confirmed that PMA and HK E. coli induced an inflammatory response in the Jurkat T-cells.
T65 3755-3858 Sentence denotes These results confirmed that PMA and HK E. coli induced an inflammatory response in the Jurkat T-cells.
T3936 3859-3956 Sentence denotes It is interesting to note that PMA was 120-fold more effective at inducing CXCL8 than HK E. coli.
T66 3859-3956 Sentence denotes It is interesting to note that PMA was 120-fold more effective at inducing CXCL8 than HK E. coli.
T3937 3957-4070 Sentence denotes PMA-dependent induction of AP-1 and down-regulation of NF-κB suggests an involvement of AP-1 in CXCL8 regulation.
T67 3957-4070 Sentence denotes PMA-dependent induction of AP-1 and down-regulation of NF-κB suggests an involvement of AP-1 in CXCL8 regulation.
T3938 4071-4258 Sentence denotes Determination of the time course of cytokine induction in response to PMA showed that CXCL8 was already released between 2-6 h, while TNF and IL-6 were released between 6-24 h (figure 3).
T68 4071-4258 Sentence denotes Determination of the time course of cytokine induction in response to PMA showed that CXCL8 was already released between 2-6 h, while TNF and IL-6 were released between 6-24 h (figure 3).
T3939 4259-4416 Sentence denotes These results indicated that the cytokines were differentially regulated and that the release was not associated with the early transient induction of NF-κB.
T69 4259-4416 Sentence denotes These results indicated that the cytokines were differentially regulated and that the release was not associated with the early transient induction of NF-κB.
T3940 4417-4517 Sentence denotes The temporal induction of AP-1 correlated to the CXCL8 levels and preceded the TNF and IL-6 release.
T70 4417-4517 Sentence denotes The temporal induction of AP-1 correlated to the CXCL8 levels and preceded the TNF and IL-6 release.
T3941 4518-4589 Sentence denotes This suggests an association between CXCL8 release and AP-1 signalling.
T71 4518-4589 Sentence denotes This suggests an association between CXCL8 release and AP-1 signalling.
T72 4590-4672 Sentence denotes Figure 3 CXCL8, IL-6 and TNF expression following long-term stimulation with PMA.
T15595 4600-4672 Sentence denotes CXCL8, IL-6 and TNF expression following long-term stimulation with PMA.
T15596 4673-4809 Sentence denotes Jurkat T-cells were either treated with media (white bars) or stimulated with PMA (black bars) and incubated for 1 h, 2 h, 6 h and 24 h.
T73 4673-4809 Sentence denotes Jurkat T-cells were either treated with media (white bars) or stimulated with PMA (black bars) and incubated for 1 h, 2 h, 6 h and 24 h.
T15597 4810-4933 Sentence denotes Aged media was added following each centrifugation step representative to the stimulation time (see Materials and methods).
T74 4810-4933 Sentence denotes Aged media was added following each centrifugation step representative to the stimulation time (see Materials and methods).
T15598 4934-5005 Sentence denotes Cytokine levels (A) CXCL8, (B) IL-6 and (C) TNF were detected by ELISA.
T75 4934-5005 Sentence denotes Cytokine levels (A) CXCL8, (B) IL-6 and (C) TNF were detected by ELISA.
T15599 5006-5095 Sentence denotes Statistical significance from the control was determined using Student's t-test. (n = 3).
T76 5006-5095 Sentence denotes Statistical significance from the control was determined using Student's t-test. (n = 3).
T77 5096-5191 Sentence denotes Table 1 Jurkat T-cells were stimulated with 162 nM PMA and 5 × 107 CFU/ml HK E. coli for 24 h.
T17005 5105-5191 Sentence denotes Jurkat T-cells were stimulated with 162 nM PMA and 5 × 107 CFU/ml HK E. coli for 24 h.
T78 5193-5248 Sentence denotes Cytokine levels were determined using multiplex assays.
T79 5249-5323 Sentence denotes Concentrations are given as pg/ml for each group (mean value ± SD, n = 3).
T80 5324-5408 Sentence denotes Statistical significance from the control (C) was determined using Student's t-test.
T4753 5410-5462 Sentence denotes Cooperative induction of cytokines by AP-1 and NF-κB
T81 5410-5462 Sentence denotes Cooperative induction of cytokines by AP-1 and NF-κB
T4754 5463-5594 Sentence denotes To further characterize the involvement of NF-κB in cytokine regulation, we treated cells with an NF-κB activation inhibitor (NAI).
T82 5463-5594 Sentence denotes To further characterize the involvement of NF-κB in cytokine regulation, we treated cells with an NF-κB activation inhibitor (NAI).
T4755 5595-5739 Sentence denotes The results showed that NAI selectively down-regulated NF-κB activation (figures 4a and 4b) and did not alter AP-1 activity (figures 4c and 4d).
T83 5595-5739 Sentence denotes The results showed that NAI selectively down-regulated NF-κB activation (figures 4a and 4b) and did not alter AP-1 activity (figures 4c and 4d).
T4756 5740-5921 Sentence denotes Exposure of Jurkat T-cells to NAI resulted in a modest reduction of CXCL8 following PMA exposure, while it did not alter the CXCL8 release following HK E. coli exposure (figure 5a).
T84 5740-5921 Sentence denotes Exposure of Jurkat T-cells to NAI resulted in a modest reduction of CXCL8 following PMA exposure, while it did not alter the CXCL8 release following HK E. coli exposure (figure 5a).
T4757 5922-6094 Sentence denotes NAI did not affect TNF expression (figure 5b) indicating that NF-κB is not the main regulator of CXCL8 or TNF following either PMA or HK E. coli exposure in Jurkat T-cells.
T85 5922-6094 Sentence denotes NAI did not affect TNF expression (figure 5b) indicating that NF-κB is not the main regulator of CXCL8 or TNF following either PMA or HK E. coli exposure in Jurkat T-cells.
T4758 6095-6295 Sentence denotes In contrast, NAI resulted in a complete inhibition of IL-6 following PMA exposure and a 45% inhibition following HK E. coli exposure (figure 5c), suggesting an involvement of NF-κB in IL-6 regulation.
T86 6095-6295 Sentence denotes In contrast, NAI resulted in a complete inhibition of IL-6 following PMA exposure and a 45% inhibition following HK E. coli exposure (figure 5c), suggesting an involvement of NF-κB in IL-6 regulation.
T87 6296-6342 Sentence denotes Figure 4 Inhibition of NF-κB activity by NAI.
T15871 6306-6342 Sentence denotes Inhibition of NF-κB activity by NAI.
T15872 6343-6461 Sentence denotes Jurkat T-cells were transfected with luciferase reporter plasmids containing either NF-κB or AP-1 cis-acting elements.
T88 6343-6461 Sentence denotes Jurkat T-cells were transfected with luciferase reporter plasmids containing either NF-κB or AP-1 cis-acting elements.
T15873 6462-6682 Sentence denotes The cells were incubated with NF-κB activation inhibitor (NAI) for 1 h followed by stimulation with PMA (162 nM, A and C) or HK E. coli (5 × 107 CFU/ml, B and D) for 24 h. (A, B) NF-κB, but not (C, D) AP-1 was inhibited.
T89 6462-6682 Sentence denotes The cells were incubated with NF-κB activation inhibitor (NAI) for 1 h followed by stimulation with PMA (162 nM, A and C) or HK E. coli (5 × 107 CFU/ml, B and D) for 24 h. (A, B) NF-κB, but not (C, D) AP-1 was inhibited.
T15874 6683-6798 Sentence denotes Statistical significance from the positive control (PMA/HK E. coli) was determined using Student's t-test. (n = 4).
T90 6683-6798 Sentence denotes Statistical significance from the positive control (PMA/HK E. coli) was determined using Student's t-test. (n = 4).
T15875 6799-6822 Sentence denotes Controls were set to 1.
T91 6799-6822 Sentence denotes Controls were set to 1.
T92 6823-6877 Sentence denotes Figure 5 Involvement of NF-κB in cytokine regulation.
T16165 6833-6877 Sentence denotes Involvement of NF-κB in cytokine regulation.
T16166 6878-7474 Sentence denotes Cytokine/chemokine levels were determined using ELISA following incubation of Jurkat T-cells with NAI (1 h) and stimulation (24 h). (A) CXCL8 expression was partially inhibited following PMA stimulation (grey bars), whereas the levels were not altered following stimulation with HK E. coli (black bars), this indicates an induction mainly regulated by AP-1. (B) TNF expression was not affected by NAI. (C) IL-6 release was completely inhibited by NAI following PMA exposure, indicating regulation through NF-κB since IL-6 expression was significantly increased in response to HK E. coli than PMA.
T93 6878-7474 Sentence denotes Cytokine/chemokine levels were determined using ELISA following incubation of Jurkat T-cells with NAI (1 h) and stimulation (24 h). (A) CXCL8 expression was partially inhibited following PMA stimulation (grey bars), whereas the levels were not altered following stimulation with HK E. coli (black bars), this indicates an induction mainly regulated by AP-1. (B) TNF expression was not affected by NAI. (C) IL-6 release was completely inhibited by NAI following PMA exposure, indicating regulation through NF-κB since IL-6 expression was significantly increased in response to HK E. coli than PMA.
T16167 7475-7590 Sentence denotes Statistical significance from the positive control (PMA/HK E. coli) was determined using Student's t-test. (n = 3).
T94 7475-7590 Sentence denotes Statistical significance from the positive control (PMA/HK E. coli) was determined using Student's t-test. (n = 3).
T4759 7593-7802 Sentence denotes Ca2+ was observed to increase AP-1 activity (figure 1c) and reduce NF-κB activity (figure 2e); therefore, we exposed T-cells to a PKC inhibitor together with PMA to determine its effect on cytokine expression.
T95 7593-7802 Sentence denotes Ca2+ was observed to increase AP-1 activity (figure 1c) and reduce NF-κB activity (figure 2e); therefore, we exposed T-cells to a PKC inhibitor together with PMA to determine its effect on cytokine expression.
T4760 7803-7926 Sentence denotes Inhibition of PKC reduced CXCL8 release from 7 ng/ml to 3 ng/ml while it had a modest effect on IL-6 and TNF (figure 6a-c).
T96 7803-7926 Sentence denotes Inhibition of PKC reduced CXCL8 release from 7 ng/ml to 3 ng/ml while it had a modest effect on IL-6 and TNF (figure 6a-c).
T4761 7927-8016 Sentence denotes This prompted us to test the effect of JNK inhibition on PMA-induced cytokine expression.
T97 7927-8016 Sentence denotes This prompted us to test the effect of JNK inhibition on PMA-induced cytokine expression.
T4762 8017-8207 Sentence denotes JNK is involved in the regulation of a multitude of different transcription factors, including the phosphorylation and activation of c-Jun, c-Fos and p53, leading to cellular apoptosis [26].
T98 8017-8207 Sentence denotes JNK is involved in the regulation of a multitude of different transcription factors, including the phosphorylation and activation of c-Jun, c-Fos and p53, leading to cellular apoptosis [26].
T4763 8208-8360 Sentence denotes Inhibition of the JNK pathways resulted in a down-regulation of both CXCL8 and IL-6, while no clear effect was observed on TNF expression (figure 6a-c).
T99 8208-8360 Sentence denotes Inhibition of the JNK pathways resulted in a down-regulation of both CXCL8 and IL-6, while no clear effect was observed on TNF expression (figure 6a-c).
T4764 8361-8497 Sentence denotes Analysis of mRNA levels using RT-qPCR (table 2) showed that PMA induced both il-6 and cxcl8 mRNA (5.1-fold and 111.8 fold respectively).
T100 8361-8497 Sentence denotes Analysis of mRNA levels using RT-qPCR (table 2) showed that PMA induced both il-6 and cxcl8 mRNA (5.1-fold and 111.8 fold respectively).
T4765 8498-8603 Sentence denotes Addition of the NF-κB inhibitor NAI and the JNK inhibitor reduced the il-6 expression below basal levels.
T101 8498-8603 Sentence denotes Addition of the NF-κB inhibitor NAI and the JNK inhibitor reduced the il-6 expression below basal levels.
T4766 8604-8726 Sentence denotes In contrast, while the cxcl8 levels were suppressed by the same treatments the levels remained elevated above basal level.
T102 8604-8726 Sentence denotes In contrast, while the cxcl8 levels were suppressed by the same treatments the levels remained elevated above basal level.
T103 8727-8787 Sentence denotes Figure 6 Involvement of PKC and JNK in cytokine expression.
T16436 8737-8787 Sentence denotes Involvement of PKC and JNK in cytokine expression.
T16437 8788-9128 Sentence denotes Jurkat T-cells were incubated with PKC- and JNK inhibitors for 1 h followed by stimulation with PMA (162 nM) for 24 h. (A) CXCL8 expression was inhibited in a dose-dependent manner by both inhibitors. (B) JNK-I revealed a stronger inhibitory effect on IL-6 expression than the PKC-I. (C) TNF expression was not affected by either inhibitor.
T104 8788-9128 Sentence denotes Jurkat T-cells were incubated with PKC- and JNK inhibitors for 1 h followed by stimulation with PMA (162 nM) for 24 h. (A) CXCL8 expression was inhibited in a dose-dependent manner by both inhibitors. (B) JNK-I revealed a stronger inhibitory effect on IL-6 expression than the PKC-I. (C) TNF expression was not affected by either inhibitor.
T16438 9129-9233 Sentence denotes Statistical significance from the positive control (PMA) was determined using Student's t-test. (n = 3).
T105 9129-9233 Sentence denotes Statistical significance from the positive control (PMA) was determined using Student's t-test. (n = 3).
T106 9234-9368 Sentence denotes Table 2 Jurkat T-cells were treated with 10 nM NAI, 10 μM JNK-I or10 nM PKC-I for 2 h followed by induction with 162 nM PMA for 24 h.
T17046 9243-9368 Sentence denotes Jurkat T-cells were treated with 10 nM NAI, 10 μM JNK-I or10 nM PKC-I for 2 h followed by induction with 162 nM PMA for 24 h.
T107 9370-9415 Sentence denotes Gene expression was determined using RT-qPCR.
T108 9416-9458 Sentence denotes The Ct values were normalized against 18S.
T109 9459-9554 Sentence denotes Statistical significance from the positive control (PMA) was determined using Student's t-test.
T110 9555-9632 Sentence denotes Concentrations are given as fg/ml, values are presented as mean ± SEM, n = 3.
T5994 9634-9689 Sentence denotes NF-κB inhibition due to PKC-dependent Bcl10 degradation
T111 9634-9689 Sentence denotes NF-κB inhibition due to PKC-dependent Bcl10 degradation
T5995 9690-9885 Sentence denotes Western blot analysis revealed an up-regulation of phosphorylated-PKC after 24 h treatment of Jurkat T-cells with PMA or HK E. coli (figure 7a), while IκBβ levels remained unaffected (figure 7b).
T112 9690-9885 Sentence denotes Western blot analysis revealed an up-regulation of phosphorylated-PKC after 24 h treatment of Jurkat T-cells with PMA or HK E. coli (figure 7a), while IκBβ levels remained unaffected (figure 7b).
T5996 9886-10052 Sentence denotes Bcl10 is a signalling protein that acts upstream of NF-κB in concert with CARMA1 and MALT1 and has been suggested to directly regulate NF-κB activity in T-cells [27].
T113 9886-10052 Sentence denotes Bcl10 is a signalling protein that acts upstream of NF-κB in concert with CARMA1 and MALT1 and has been suggested to directly regulate NF-κB activity in T-cells [27].
T5997 10053-10197 Sentence denotes Therefore, Bcl10 activation was evaluated in both control and PMA stimulated cells after 10 min, 1 h, 6 h, and 24 h using western blot analysis.
T114 10053-10197 Sentence denotes Therefore, Bcl10 activation was evaluated in both control and PMA stimulated cells after 10 min, 1 h, 6 h, and 24 h using western blot analysis.
T5998 10198-10331 Sentence denotes The Bcl10 levels decreased following treatment with PMA, while in control cells, Bcl10 returned to higher levels by 24 h (figure 7c).
T115 10198-10331 Sentence denotes The Bcl10 levels decreased following treatment with PMA, while in control cells, Bcl10 returned to higher levels by 24 h (figure 7c).
T5999 10332-10421 Sentence denotes This suggests that Bcl10 is involved in the PMA dependent inhibition of NF-κB activation.
T116 10332-10421 Sentence denotes This suggests that Bcl10 is involved in the PMA dependent inhibition of NF-κB activation.
T117 10422-10502 Sentence denotes Figure 7 NF-κB inhibition by PMA correlated to PKC-dependent Bcl10 degradation.
T16732 10432-10502 Sentence denotes NF-κB inhibition by PMA correlated to PKC-dependent Bcl10 degradation.
T16733 10503-10973 Sentence denotes Levels of intracellular protein were assessed following 24 h stimulation with PMA (162 nM) or HK E. coli (5 × 107 CFU/ml). (A) Phospho-PKC increased in response to PMA and HK E. coli stimulation. (B) IκB decreased following stimulation with HK E. coli indicating NF-κB activation. (C) Bcl10 activation was inhibited following long-term stimulation with PMA, which explains the inhibitory effect of PMA on NF-κB activation. β-actin was used as a loading control. (n = 3).
T118 10503-10973 Sentence denotes Levels of intracellular protein were assessed following 24 h stimulation with PMA (162 nM) or HK E. coli (5 × 107 CFU/ml). (A) Phospho-PKC increased in response to PMA and HK E. coli stimulation. (B) IκB decreased following stimulation with HK E. coli indicating NF-κB activation. (C) Bcl10 activation was inhibited following long-term stimulation with PMA, which explains the inhibitory effect of PMA on NF-κB activation. β-actin was used as a loading control. (n = 3).