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PMC:2664230 / 145-2097 JSONTXT 17 Projects

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Id Subject Object Predicate Lexical cue
T112 0-9 Sentence denotes OBJECTIVE
T4 0-9 Sentence denotes OBJECTIVE
T113 10-259 Sentence denotes The objective of this study was to determine the effect of nonspecific phosphodiesterase inhibition on transcription factor activation and tumor necrosis factor-alpha (TNF-α) production in lipopolysaccharide (LPS)-stimulated human mononuclear cells.
T5 10-259 Sentence denotes The objective of this study was to determine the effect of nonspecific phosphodiesterase inhibition on transcription factor activation and tumor necrosis factor-alpha (TNF-α) production in lipopolysaccharide (LPS)-stimulated human mononuclear cells.
T114 261-273 Sentence denotes INTRODUCTION
T6 261-273 Sentence denotes INTRODUCTION
T115 274-385 Sentence denotes The production of TNF-α following LPS stimulation is one of the key steps in bacterial sepsis and inflammation.
T7 274-385 Sentence denotes The production of TNF-α following LPS stimulation is one of the key steps in bacterial sepsis and inflammation.
T116 386-501 Sentence denotes The mechanism by which phosphodiesterase inhibition alters TNF-α production in the presence of LPS remains unclear.
T8 386-501 Sentence denotes The mechanism by which phosphodiesterase inhibition alters TNF-α production in the presence of LPS remains unclear.
T117 503-510 Sentence denotes METHODS
T9 503-510 Sentence denotes METHODS
T118 511-673 Sentence denotes Human mononuclear cells were stimulated with LPS (1 μg/mL), in the presence and absence of Pentoxifylline (PTX; 20 mM), a nonspecific phosphodiesterase inhibitor.
T10 511-673 Sentence denotes Human mononuclear cells were stimulated with LPS (1 μg/mL), in the presence and absence of Pentoxifylline (PTX; 20 mM), a nonspecific phosphodiesterase inhibitor.
T119 674-830 Sentence denotes Western blotting of phosphorylated cytoplasmic I-κBα, nuclear factor-κB p65 (NF-κB), and nuclear cAMP-response element binding protein (CREB) was performed.
T11 674-830 Sentence denotes Western blotting of phosphorylated cytoplasmic I-κBα, nuclear factor-κB p65 (NF-κB), and nuclear cAMP-response element binding protein (CREB) was performed.
T120 831-914 Sentence denotes DNA binding of NF-κB and CREB was verified by electrophoretic mobility shift assay.
T12 831-914 Sentence denotes DNA binding of NF-κB and CREB was verified by electrophoretic mobility shift assay.
T121 915-1087 Sentence denotes TNF-α levels were determined in the supernatant of stimulated cells in the presence and absence Protein kinase A inhibition by an enzyme-linked immunosorbent assay (ELISA).
T13 915-1087 Sentence denotes TNF-α levels were determined in the supernatant of stimulated cells in the presence and absence Protein kinase A inhibition by an enzyme-linked immunosorbent assay (ELISA).
T122 1089-1096 Sentence denotes RESULTS
T14 1089-1096 Sentence denotes RESULTS
T123 1097-1244 Sentence denotes PTX was demonstrated to significantly reduce cytoplasmic I-κBα phosphorylation, nuclear p65 phosphorylation, and the DNA binding activity of NF-κB.
T15 1097-1244 Sentence denotes PTX was demonstrated to significantly reduce cytoplasmic I-κBα phosphorylation, nuclear p65 phosphorylation, and the DNA binding activity of NF-κB.
T124 1245-1333 Sentence denotes In contrast, PTX markedly enhanced the phosphorylation and DNA binding activity of CREB.
T16 1245-1333 Sentence denotes In contrast, PTX markedly enhanced the phosphorylation and DNA binding activity of CREB.
T125 1334-1468 Sentence denotes Cells concomitantly treated with PTX and LPS secreted similar levels of TNF-α in the presence and absence Protein kinase A inhibition.
T17 1334-1468 Sentence denotes Cells concomitantly treated with PTX and LPS secreted similar levels of TNF-α in the presence and absence Protein kinase A inhibition.
T126 1470-1480 Sentence denotes DISCUSSION
T18 1470-1480 Sentence denotes DISCUSSION
T127 1481-1754 Sentence denotes The increased level of cAMP that results from phosphodiesterase inhibition affects cytoplasmic and nuclear events, resulting in the attenuation of NF-κB and the activation of CREB transcriptional DNA binding through pathways that are partially Protein kinase A-independent.
T19 1481-1754 Sentence denotes The increased level of cAMP that results from phosphodiesterase inhibition affects cytoplasmic and nuclear events, resulting in the attenuation of NF-κB and the activation of CREB transcriptional DNA binding through pathways that are partially Protein kinase A-independent.
T128 1756-1766 Sentence denotes CONCLUSION
T20 1756-1766 Sentence denotes CONCLUSION
T129 1767-1951 Sentence denotes PTX-mediated phosphodiesterase inhibition occurs partially through a Protein kinase A-independent pathway and may serve as a useful tool in the attenuation of LPS-induced inflammation.
T21 1767-1951 Sentence denotes PTX-mediated phosphodiesterase inhibition occurs partially through a Protein kinase A-independent pathway and may serve as a useful tool in the attenuation of LPS-induced inflammation.