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PMC:1310901 / 19754-20782 JSONTXT 15 Projects

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Id Subject Object Predicate Lexical cue
T9690 0-83 Sentence denotes In vitro methylation of an IRF-4 promoter-reporter construct decreases its activity
T141 0-83 Sentence denotes In vitro methylation of an IRF-4 promoter-reporter construct decreases its activity
T9691 84-284 Sentence denotes To provide evidence for a direct effect of methylational status on IRF-4 promoter activity we performed reporter gene assays with IRF-4 promoter constructs before and after their in vitro methylation.
T142 84-284 Sentence denotes To provide evidence for a direct effect of methylational status on IRF-4 promoter activity we performed reporter gene assays with IRF-4 promoter constructs before and after their in vitro methylation.
T9692 285-416 Sentence denotes A complete methylation of these constructs was checked via restriction assays with methylation-sensitive endonucleases (Figure 5A).
T143 285-416 Sentence denotes A complete methylation of these constructs was checked via restriction assays with methylation-sensitive endonucleases (Figure 5A).
T9693 417-557 Sentence denotes Intriguingly, methylation of the IRF-4 promoter significantly decreased promoter activity in IRF-4-positive SD-1 cells by 85.0% (Figure 5B).
T144 417-557 Sentence denotes Intriguingly, methylation of the IRF-4 promoter significantly decreased promoter activity in IRF-4-positive SD-1 cells by 85.0% (Figure 5B).
T9694 558-759 Sentence denotes The silencing effect of CpG methylation was not restricted to IRF-4-positive cells, since in vitro methylation led to a 92.9% abrogation of promoter activity in IRF-4-negative Jurkat cells (Figure 5C).
T145 558-759 Sentence denotes The silencing effect of CpG methylation was not restricted to IRF-4-positive cells, since in vitro methylation led to a 92.9% abrogation of promoter activity in IRF-4-negative Jurkat cells (Figure 5C).
T9695 760-933 Sentence denotes In contrast, control methylation of a reporter construct with a different promoter (FasL) as well as an empty vector had no effect on the reporter activity (data not shown).
T146 760-933 Sentence denotes In contrast, control methylation of a reporter construct with a different promoter (FasL) as well as an empty vector had no effect on the reporter activity (data not shown).
T9696 934-1028 Sentence denotes These data proved a direct association between methylation and activity of the IRF-4 promoter.
T147 934-1028 Sentence denotes These data proved a direct association between methylation and activity of the IRF-4 promoter.