pubmed-sentences-benchmark  

PubMed:8662666 JSONTXT 32 Projects

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Id Subject Object Predicate Lexical cue
S1 0-125 Sentence denotes DNA triplex formation selectively inhibits granulocyte-macrophage colony-stimulating factor gene expression in human T cells.
S2 126-303 Sentence denotes Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a hemopoietic growth factor that is expressed in activated T cells, fibroblasts, macrophages, and endothelial cells.
S3 304-508 Sentence denotes Although GM-CSF does not appear to be essential for normal hemopoiesis, overexpression of GM-CSF has been implicated in the pathogenesis of some diseases such as myeloid leukemia and chronic inflammation.
S4 509-714 Sentence denotes An NF-kappaB/Rel binding site within the GM-CSF promoter, termed the kappaB element appears to be important for controlling expression in reporter gene assays in response to a number of stimuli in T cells.
S5 715-868 Sentence denotes We investigated oligonucleotide-directed triple helix formation across this regulatory sequence as a potential tool to inhibit GM-CSF gene transcription.
S6 869-1005 Sentence denotes A 15-base oligonucleotide, GM3, was targeted to a purine-rich region in the GM-CSF proximal promoter, which overlaps the kappaB element.
S7 1006-1160 Sentence denotes Gel mobility shift assays and DNase I footprinting demonstrated that GM3 formed a sequence-specific collinear triplex with its double-stranded DNA target.
S8 1161-1267 Sentence denotes Triplex formation by GM3 blocked recombinant and nuclear NF-kappaB proteins binding to the GM-CSF element.
S9 1268-1468 Sentence denotes GM3 also caused selective inhibition of the human T-cell lymphotrophic virus-1 Tax transactivator-induced luciferase activity from a reporter construct driven by the GM-CSF promoter in Jurkat T cells.
S10 1469-1652 Sentence denotes Finally, GM3 greatly reduced the concentration of endogenous GM-CSF mRNA induced by different stimuli in Jurkat T cells but did not affect interleukin 3 mRNA levels in the same cells.
S11 1653-1797 Sentence denotes We conclude that the kappaB element in the GM-CSF promoter plays a central role in the transcriptional activation of the endogenous GM-CSF gene.
S12 1798-1992 Sentence denotes Colinear triplex formation acts as a selective transcriptional repressor of the GM-CSF gene and may have potential therapeutic application in cases of undesirable overexpression of this protein.