| Id |
Subject |
Object |
Predicate |
Lexical cue |
| S1 |
0-91 |
Sentence |
denotes |
T-cell expression of the human GATA-3 gene is regulated by a non-lineage-specific silencer. |
| S2 |
92-222 |
Sentence |
denotes |
The GATA-3 transcription factor is required for development of the T-cell lineage and Th2 cytokine gene expression in CD4 T-cells. |
| S3 |
223-375 |
Sentence |
denotes |
We have mapped the DNase-I-hypersensitive (HS) regions of the human GATA-3 gene in T-cells and non-T-cells and studied their transcriptional activities. |
| S4 |
376-600 |
Sentence |
denotes |
HS I-III, located 5' from the transcriptional initiation site, were found in hematopoietic and non-hematopoietic cells, whereas HS IV-VII, located 3' from the transcriptional start site, were exclusively observed in T-cells. |
| S5 |
601-822 |
Sentence |
denotes |
Among these hypersensitive sites, two transcriptional control elements were found, one in the first intron of the GATA-3 gene and the other between 8.3 and 5.9 kilobases 5' from the GATA-3 transcriptional initiation site. |
| S6 |
823-949 |
Sentence |
denotes |
The first intron acted as a strong transcriptional activator in a position-dependent manner and with no cell-type specificity. |
| S7 |
950-1176 |
Sentence |
denotes |
The upstream regulatory element could confer T-cell specificity to the GATA-3 promoter activity, and analysis of this region revealed a 707-base pair silencer that drastically inhibited GATA-3 promoter activity in non-T-cells. |
| S8 |
1177-1287 |
Sentence |
denotes |
Two CAGGTG E-boxes, located at the 5'- and 3'-ends of the silencer, were necessary for this silencer activity. |
| S9 |
1288-1518 |
Sentence |
denotes |
The 3'-CAGGTG E-box could bind USF proteins, the ubiquitous repressor ZEB, or the basic helix-loop-helix proteins E2A and HEB, and we showed that a competition between ZEB and E2A/HEB proteins is involved in the silencer activity. |
