Id |
Subject |
Object |
Predicate |
Lexical cue |
T3389 |
0-3 |
CD |
denotes |
2.6 |
T3390 |
5-12 |
NNP |
denotes |
Western |
T3391 |
13-17 |
NNP |
denotes |
Blot |
T3392 |
18-20 |
TO |
denotes |
To |
T3393 |
21-27 |
VB |
denotes |
obtain |
T3394 |
28-33 |
JJ |
denotes |
total |
T3395 |
34-41 |
NN |
denotes |
protein |
T3396 |
42-49 |
NN |
denotes |
lysates |
T3397 |
49-50 |
-COMMA- |
denotes |
, |
T3398 |
51-56 |
NN |
denotes |
cells |
T3399 |
57-61 |
VB |
denotes |
were |
T3400 |
62-73 |
VB |
denotes |
homogenized |
T3401 |
74-76 |
IN |
denotes |
in |
T3402 |
77-81 |
NN |
denotes |
RIPA |
T3403 |
82-88 |
NN |
denotes |
buffer |
T3404 |
89-90 |
-LRB- |
denotes |
( |
T3405 |
90-91 |
CD |
denotes |
1 |
T3406 |
91-92 |
NN |
denotes |
% |
T3407 |
93-97 |
NN |
denotes |
NP40 |
T3408 |
97-98 |
-COMMA- |
denotes |
, |
T3409 |
99-102 |
CD |
denotes |
0.5 |
T3410 |
102-103 |
NN |
denotes |
% |
T3411 |
104-110 |
NN |
denotes |
sodium |
T3412 |
111-123 |
NN |
denotes |
deoxycholate |
T3413 |
123-124 |
-COMMA- |
denotes |
, |
T3414 |
125-128 |
CD |
denotes |
0.1 |
T3415 |
128-129 |
NN |
denotes |
% |
T3416 |
130-133 |
NN |
denotes |
SDS |
T3417 |
133-134 |
-COMMA- |
denotes |
, |
T3418 |
135-136 |
CD |
denotes |
1 |
T3419 |
137-139 |
NN |
denotes |
mM |
T3420 |
140-144 |
NN |
denotes |
EDTA |
T3421 |
144-145 |
-COMMA- |
denotes |
, |
T3422 |
146-147 |
CD |
denotes |
1 |
T3423 |
148-150 |
NN |
denotes |
mM |
T3424 |
151-155 |
NN |
denotes |
EGTA |
T3425 |
155-156 |
-COMMA- |
denotes |
, |
T3426 |
157-158 |
CD |
denotes |
1 |
T3427 |
159-161 |
NN |
denotes |
mM |
T3428 |
162-168 |
NN |
denotes |
Na3VO4 |
T3429 |
168-169 |
-COMMA- |
denotes |
, |
T3430 |
170-172 |
CD |
denotes |
20 |
T3431 |
173-175 |
NN |
denotes |
mM |
T3432 |
176-179 |
NN |
denotes |
NaF |
T3433 |
179-180 |
-COMMA- |
denotes |
, |
T3434 |
181-184 |
CD |
denotes |
0.5 |
T3435 |
185-187 |
NN |
denotes |
mM |
T3436 |
188-191 |
NN |
denotes |
DTT |
T3437 |
191-192 |
-COMMA- |
denotes |
, |
T3438 |
193-194 |
CD |
denotes |
1 |
T3439 |
195-197 |
NN |
denotes |
mM |
T3440 |
198-202 |
NN |
denotes |
PMSF |
T3441 |
202-203 |
-COMMA- |
denotes |
, |
T3442 |
204-207 |
CC |
denotes |
and |
T3443 |
208-216 |
NN |
denotes |
protease |
T3444 |
217-226 |
NN |
denotes |
inhibitor |
T3445 |
227-235 |
NN |
denotes |
cocktail |
T3446 |
236-238 |
IN |
denotes |
in |
T3447 |
239-242 |
NNP |
denotes |
PBS |
T3448 |
243-245 |
NN |
denotes |
pH |
T3449 |
246-249 |
CD |
denotes |
7.4 |
T3450 |
249-250 |
-RRB- |
denotes |
) |
T3451 |
251-254 |
CC |
denotes |
and |
T3452 |
255-266 |
VB |
denotes |
centrifuged |
T3453 |
267-269 |
IN |
denotes |
at |
T3454 |
270-276 |
CD |
denotes |
12,000 |
T3455 |
277-278 |
NN |
denotes |
g |
T3456 |
279-282 |
IN |
denotes |
for |
T3457 |
283-285 |
CD |
denotes |
15 |
T3458 |
286-289 |
NN |
denotes |
min |
T3459 |
290-292 |
IN |
denotes |
at |
T3460 |
293-296 |
NN |
denotes |
4°C |
T3461 |
298-305 |
NN |
denotes |
Protein |
T3462 |
306-320 |
NN |
denotes |
concentrations |
T3463 |
321-325 |
VB |
denotes |
were |
T3464 |
326-335 |
VB |
denotes |
estimated |
T3465 |
336-338 |
IN |
denotes |
by |
T3466 |
339-348 |
NNP |
denotes |
Coomassie |
T3467 |
349-353 |
NNP |
denotes |
Plus |
T3468 |
354-361 |
NNP |
denotes |
Protein |
T3469 |
362-367 |
NNP |
denotes |
Assay |
T3470 |
368-375 |
NNP |
denotes |
Reagent |
T3471 |
376-377 |
-LRB- |
denotes |
( |
T3472 |
377-383 |
NNP |
denotes |
Pierce |
T3473 |
383-384 |
-COMMA- |
denotes |
, |
T3474 |
385-387 |
NNP |
denotes |
IL |
T3475 |
387-388 |
-COMMA- |
denotes |
, |
T3476 |
389-392 |
NNP |
denotes |
USA |
T3477 |
392-393 |
-RRB- |
denotes |
) |
T3478 |
395-400 |
CD |
denotes |
Fifty |
T3479 |
401-411 |
NN |
denotes |
micrograms |
T3480 |
412-414 |
IN |
denotes |
of |
T3481 |
415-418 |
DT |
denotes |
the |
T3482 |
419-428 |
VB |
denotes |
resulting |
T3483 |
429-438 |
JJ |
denotes |
cytosolic |
T3484 |
439-446 |
NN |
denotes |
protein |
T3485 |
447-455 |
NN |
denotes |
extracts |
T3486 |
456-460 |
VB |
denotes |
were |
T3487 |
461-475 |
JJ |
denotes |
heat-denatured |
T3488 |
476-478 |
IN |
denotes |
in |
T3489 |
479-486 |
NNP |
denotes |
Laemmli |
T3490 |
487-493 |
NN |
denotes |
sample |
T3491 |
494-501 |
NN |
denotes |
loading |
T3492 |
502-508 |
NN |
denotes |
buffer |
T3493 |
508-509 |
-COMMA- |
denotes |
, |
T3494 |
510-519 |
VB |
denotes |
separated |
T3495 |
520-522 |
IN |
denotes |
by |
T3496 |
523-525 |
CD |
denotes |
10 |
T3497 |
525-526 |
NN |
denotes |
% |
T3498 |
527-533 |
NN |
denotes |
sodium |
T3499 |
534-541 |
NN |
denotes |
dodecyl |
T3500 |
542-549 |
NN |
denotes |
sulfate |
T3501 |
550-564 |
NN |
denotes |
polyacrylamide |
T3502 |
565-568 |
NN |
denotes |
gel |
T3503 |
569-584 |
NN |
denotes |
electrophoresis |
T3504 |
584-585 |
-COMMA- |
denotes |
, |
T3505 |
586-589 |
CC |
denotes |
and |
T3506 |
590-604 |
VB |
denotes |
electroblotted |
T3507 |
605-609 |
IN |
denotes |
onto |
T3508 |
610-611 |
DT |
denotes |
a |
T3509 |
612-626 |
NN |
denotes |
nitrocellulose |
T3510 |
627-635 |
NN |
denotes |
membrane |
T3511 |
637-640 |
IN |
denotes |
For |
T3512 |
641-655 |
NN |
denotes |
immunoblotting |
T3513 |
655-656 |
-COMMA- |
denotes |
, |
T3514 |
657-666 |
NN |
denotes |
membranes |
T3515 |
667-671 |
VB |
denotes |
were |
T3516 |
672-679 |
VB |
denotes |
blocked |
T3517 |
680-684 |
IN |
denotes |
with |
T3518 |
685-686 |
CD |
denotes |
5 |
T3519 |
686-687 |
NN |
denotes |
% |
T3520 |
688-694 |
JJ |
denotes |
nonfat |
T3521 |
695-698 |
JJ |
denotes |
dry |
T3522 |
699-703 |
NN |
denotes |
milk |
T3523 |
704-706 |
IN |
denotes |
in |
T3524 |
707-713 |
NN |
denotes |
saline |
T3525 |
714-720 |
NN |
denotes |
buffer |
T3526 |
721-730 |
RB |
denotes |
overnight |
T3527 |
731-733 |
IN |
denotes |
at |
T3528 |
734-737 |
NN |
denotes |
4°C |
T3529 |
737-738 |
-COMMA- |
denotes |
, |
T3530 |
739-742 |
CC |
denotes |
and |
T3531 |
743-746 |
DT |
denotes |
the |
T3532 |
747-756 |
VB |
denotes |
following |
T3533 |
757-767 |
NN |
denotes |
antibodies |
T3534 |
768-772 |
VB |
denotes |
were |
T3535 |
773-777 |
VB |
denotes |
used |
T3536 |
777-778 |
-COLON- |
denotes |
: |
T3537 |
779-791 |
JJ |
denotes |
anti-β-actin |
T3538 |
792-793 |
-LRB- |
denotes |
( |
T3539 |
793-802 |
NN |
denotes |
sc-130657 |
T3540 |
802-803 |
-COMMA- |
denotes |
, |
T3541 |
804-809 |
NNP |
denotes |
Santa |
T3542 |
810-814 |
NNP |
denotes |
Cruz |
T3543 |
815-828 |
NNP |
denotes |
Biotechnology |
T3544 |
828-829 |
-COMMA- |
denotes |
, |
T3545 |
830-832 |
NNP |
denotes |
CA |
T3546 |
832-833 |
-COMMA- |
denotes |
, |
T3547 |
834-837 |
NNP |
denotes |
USA |
T3548 |
837-838 |
-COMMA- |
denotes |
, |
T3549 |
839-841 |
CD |
denotes |
43 |
T3550 |
842-845 |
NN |
denotes |
kDa |
T3551 |
845-846 |
-RRB- |
denotes |
) |
T3552 |
847-850 |
CC |
denotes |
and |
T3553 |
851-862 |
JJ |
denotes |
anti-matrix |
T3554 |
863-879 |
NN |
denotes |
metallopeptidase |
T3555 |
880-881 |
CD |
denotes |
9 |
T3556 |
882-883 |
-LRB- |
denotes |
( |
T3557 |
883-887 |
NN |
denotes |
MMP9 |
T3558 |
887-888 |
-RRB- |
denotes |
) |
T3559 |
889-890 |
-LRB- |
denotes |
( |
T3560 |
890-897 |
NN |
denotes |
sc-6841 |
T3561 |
897-898 |
-COMMA- |
denotes |
, |
T3562 |
899-904 |
NNP |
denotes |
Santa |
T3563 |
905-909 |
NNP |
denotes |
Cruz |
T3564 |
910-923 |
NNP |
denotes |
Biotechnology |
T3565 |
923-924 |
-COMMA- |
denotes |
, |
T3566 |
925-927 |
NNP |
denotes |
CA |
T3567 |
927-928 |
-COMMA- |
denotes |
, |
T3568 |
929-932 |
NNP |
denotes |
USA |
T3569 |
932-933 |
-COMMA- |
denotes |
, |
T3570 |
934-936 |
CD |
denotes |
92 |
T3571 |
937-940 |
NN |
denotes |
kDa |
T3572 |
940-941 |
-RRB- |
denotes |
) |
T3573 |
943-947 |
DT |
denotes |
Each |
T3574 |
948-955 |
JJ |
denotes |
primary |
T3575 |
956-964 |
NN |
denotes |
antibody |
T3576 |
965-968 |
VB |
denotes |
was |
T3577 |
969-976 |
VB |
denotes |
diluted |
T3578 |
977-990 |
RB |
denotes |
appropriately |
T3579 |
991-993 |
IN |
denotes |
in |
T3580 |
994-1002 |
VB |
denotes |
blocking |
T3581 |
1003-1009 |
NN |
denotes |
buffer |
T3582 |
1010-1013 |
CC |
denotes |
and |
T3583 |
1014-1018 |
RB |
denotes |
then |
T3584 |
1019-1024 |
VB |
denotes |
added |
T3585 |
1025-1027 |
TO |
denotes |
to |
T3586 |
1028-1031 |
DT |
denotes |
the |
T3587 |
1032-1037 |
NN |
denotes |
blots |
T3588 |
1038-1041 |
IN |
denotes |
for |
T3589 |
1042-1043 |
CD |
denotes |
1 |
T3590 |
1044-1045 |
NN |
denotes |
h |
T3591 |
1046-1048 |
IN |
denotes |
at |
T3592 |
1049-1053 |
NN |
denotes |
room |
T3593 |
1054-1065 |
NN |
denotes |
temperature |
T3594 |
1067-1070 |
DT |
denotes |
The |
T3595 |
1071-1076 |
NN |
denotes |
blots |
T3596 |
1077-1081 |
VB |
denotes |
were |
T3597 |
1082-1088 |
VB |
denotes |
washed |
T3598 |
1089-1094 |
CD |
denotes |
three |
T3599 |
1095-1100 |
NN |
denotes |
times |
T3600 |
1101-1103 |
IN |
denotes |
in |
T3601 |
1104-1107 |
DT |
denotes |
the |
T3602 |
1108-1115 |
VB |
denotes |
washing |
T3603 |
1116-1122 |
NN |
denotes |
buffer |
T3604 |
1123-1126 |
CC |
denotes |
and |
T3605 |
1127-1134 |
VB |
denotes |
covered |
T3606 |
1135-1139 |
IN |
denotes |
with |
T3607 |
1140-1143 |
DT |
denotes |
the |
T3608 |
1144-1155 |
JJ |
denotes |
horseradish |
T3609 |
1156-1173 |
JJ |
denotes |
peroxidase-linked |
T3610 |
1174-1183 |
JJ |
denotes |
secondary |
T3611 |
1184-1192 |
NN |
denotes |
antibody |
T3612 |
1193-1195 |
IN |
denotes |
at |
T3613 |
1196-1197 |
DT |
denotes |
a |
T3614 |
1198-1199 |
CD |
denotes |
1 |
T3615 |
1200-1201 |
-COLON- |
denotes |
: |
T3616 |
1202-1206 |
CD |
denotes |
2000 |
T3617 |
1207-1215 |
NN |
denotes |
dilution |
T3618 |
1216-1219 |
IN |
denotes |
for |
T3619 |
1220-1221 |
CD |
denotes |
1 |
T3620 |
1222-1223 |
NN |
denotes |
h |
T3621 |
1225-1230 |
NN |
denotes |
Blots |
T3622 |
1231-1235 |
VB |
denotes |
were |
T3623 |
1236-1245 |
VB |
denotes |
incubated |
T3624 |
1246-1250 |
IN |
denotes |
with |
T3625 |
1251-1259 |
VB |
denotes |
enhanced |
T3626 |
1260-1277 |
NN |
denotes |
chemiluminescence |
T3627 |
1278-1279 |
-LRB- |
denotes |
( |
T3628 |
1279-1282 |
NN |
denotes |
ECL |
T3629 |
1282-1283 |
-RRB- |
denotes |
) |
T3630 |
1284-1293 |
NN |
denotes |
detection |
T3631 |
1294-1300 |
NN |
denotes |
system |
T3632 |
1301-1302 |
-LRB- |
denotes |
( |
T3633 |
1302-1310 |
NNP |
denotes |
Amersham |
T3634 |
1311-1322 |
NNP |
denotes |
Biosciences |
T3635 |
1322-1323 |
-COMMA- |
denotes |
, |
T3636 |
1324-1329 |
NNP |
denotes |
Bucks |
T3637 |
1329-1330 |
-COMMA- |
denotes |
, |
T3638 |
1331-1333 |
NNP |
denotes |
UK |
T3639 |
1333-1334 |
-RRB- |
denotes |
) |
T3640 |
1335-1338 |
CC |
denotes |
and |
T3641 |
1339-1346 |
VB |
denotes |
exposed |
T3642 |
1347-1349 |
TO |
denotes |
to |
T3643 |
1350-1362 |
JJ |
denotes |
radiographic |
T3644 |
1363-1367 |
NN |
denotes |
film |
T3645 |
1368-1369 |
-LRB- |
denotes |
( |
T3646 |
1369-1373 |
NNP |
denotes |
Fuji |
T3647 |
1374-1383 |
NNP |
denotes |
Hyperfilm |
T3648 |
1383-1384 |
-COMMA- |
denotes |
, |
T3649 |
1385-1390 |
NNP |
denotes |
Tokyo |
T3650 |
1390-1391 |
-COMMA- |
denotes |
, |
T3651 |
1392-1397 |
NNP |
denotes |
Japan |
T3652 |
1397-1398 |
-RRB- |
denotes |
) |
T3653 |
1400-1406 |
NN |
denotes |
ImageJ |
T3654 |
1407-1410 |
VB |
denotes |
was |
T3655 |
1411-1415 |
VB |
denotes |
used |
T3656 |
1416-1418 |
TO |
denotes |
to |
T3657 |
1419-1426 |
VB |
denotes |
analyze |
T3658 |
1427-1430 |
DT |
denotes |
the |
T3659 |
1431-1440 |
NN |
denotes |
intensity |
T3660 |
1441-1443 |
IN |
denotes |
of |
T3661 |
1444-1447 |
DT |
denotes |
the |
T3662 |
1448-1453 |
NN |
denotes |
blots |
T3663 |
1455-1458 |
DT |
denotes |
The |
T3664 |
1459-1464 |
NN |
denotes |
level |
T3665 |
1465-1467 |
IN |
denotes |
of |
T3666 |
1468-1475 |
NN |
denotes |
β-actin |
T3667 |
1476-1479 |
VB |
denotes |
was |
T3668 |
1480-1484 |
VB |
denotes |
used |
T3669 |
1485-1487 |
IN |
denotes |
as |
T3670 |
1488-1496 |
JJ |
denotes |
internal |
T3671 |
1497-1505 |
NN |
denotes |
standard |
R2899 |
T3391 |
T3390 |
arg1Of |
Blot,Western |
R2900 |
T3393 |
T3392 |
arg1Of |
obtain,To |
R2901 |
T3396 |
T3393 |
arg2Of |
lysates,obtain |
R2902 |
T3396 |
T3394 |
arg1Of |
lysates,total |
R2903 |
T3396 |
T3395 |
arg1Of |
lysates,protein |
R2904 |
T3398 |
T3393 |
arg1Of |
cells,obtain |
R2905 |
T3398 |
T3399 |
arg1Of |
cells,were |
R2906 |
T3398 |
T3400 |
arg2Of |
cells,homogenized |
R2907 |
T3398 |
T3452 |
arg2Of |
cells,centrifuged |
R2908 |
T3400 |
T3401 |
arg1Of |
homogenized,in |
R2909 |
T3400 |
T3451 |
arg1Of |
homogenized,and |
R2910 |
T3403 |
T3401 |
arg2Of |
buffer,in |
R2911 |
T3403 |
T3402 |
arg1Of |
buffer,RIPA |
R2912 |
T3403 |
T3404 |
arg1Of |
buffer,( |
R2913 |
T3405 |
T3406 |
arg1Of |
1,% |
R2914 |
T3407 |
T3405 |
arg1Of |
NP40,1 |
R2915 |
T3407 |
T3408 |
arg1Of |
NP40,"," |
R2916 |
T3408 |
T3413 |
arg1Of |
",","," |
R2917 |
T3409 |
T3410 |
arg1Of |
0.5,% |
R2918 |
T3412 |
T3408 |
arg2Of |
deoxycholate,"," |
R2919 |
T3412 |
T3409 |
arg1Of |
deoxycholate,0.5 |
R2920 |
T3412 |
T3411 |
arg1Of |
deoxycholate,sodium |
R2921 |
T3413 |
T3417 |
arg1Of |
",","," |
R2922 |
T3414 |
T3415 |
arg1Of |
0.1,% |
R2923 |
T3416 |
T3413 |
arg2Of |
SDS,"," |
R2924 |
T3416 |
T3414 |
arg1Of |
SDS,0.1 |
R2925 |
T3417 |
T3421 |
arg1Of |
",","," |
R2926 |
T3418 |
T3419 |
arg1Of |
1,mM |
R2927 |
T3420 |
T3417 |
arg2Of |
EDTA,"," |
R2928 |
T3420 |
T3418 |
arg1Of |
EDTA,1 |
R2929 |
T3421 |
T3404 |
arg2Of |
",",( |
R2930 |
T3421 |
T3425 |
arg1Of |
",","," |
R2931 |
T3422 |
T3423 |
arg1Of |
1,mM |
R2932 |
T3424 |
T3421 |
arg2Of |
EGTA,"," |
R2933 |
T3424 |
T3422 |
arg1Of |
EGTA,1 |
R2934 |
T3426 |
T3427 |
arg1Of |
1,mM |
R2935 |
T3428 |
T3426 |
arg1Of |
Na3VO4,1 |
R2936 |
T3428 |
T3429 |
arg1Of |
Na3VO4,"," |
R2937 |
T3429 |
T3433 |
arg1Of |
",","," |
R2938 |
T3430 |
T3431 |
arg1Of |
20,mM |
R2939 |
T3432 |
T3429 |
arg2Of |
NaF,"," |
R2940 |
T3432 |
T3430 |
arg1Of |
NaF,20 |
R2941 |
T3433 |
T3437 |
arg1Of |
",","," |
R2942 |
T3434 |
T3435 |
arg1Of |
0.5,mM |
R2943 |
T3436 |
T3433 |
arg2Of |
DTT,"," |
R2944 |
T3436 |
T3434 |
arg1Of |
DTT,0.5 |
R2945 |
T3437 |
T3442 |
arg1Of |
",",and |
R2946 |
T3438 |
T3439 |
arg1Of |
1,mM |
R2947 |
T3440 |
T3437 |
arg2Of |
PMSF,"," |
R2948 |
T3440 |
T3438 |
arg1Of |
PMSF,1 |
R2949 |
T3442 |
T3425 |
arg2Of |
and,"," |
R2950 |
T3442 |
T3441 |
arg1Of |
and,"," |
R2951 |
T3445 |
T3442 |
arg2Of |
cocktail,and |
R2952 |
T3445 |
T3443 |
arg1Of |
cocktail,protease |
R2953 |
T3445 |
T3444 |
arg1Of |
cocktail,inhibitor |
R2954 |
T3445 |
T3446 |
arg1Of |
cocktail,in |
R2955 |
T3448 |
T3446 |
arg2Of |
pH,in |
R2956 |
T3448 |
T3447 |
arg1Of |
pH,PBS |
R2957 |
T3448 |
T3449 |
arg1Of |
pH,7.4 |
R2958 |
T3450 |
T3404 |
arg3Of |
),( |
R2959 |
T3451 |
T3392 |
modOf |
and,To |
R2960 |
T3451 |
T3397 |
arg1Of |
and,"," |
R2961 |
T3451 |
T3399 |
arg2Of |
and,were |
R2962 |
T3452 |
T3451 |
arg2Of |
centrifuged,and |
R2963 |
T3452 |
T3453 |
arg1Of |
centrifuged,at |
R2964 |
T3452 |
T3456 |
arg1Of |
centrifuged,for |
R2965 |
T3455 |
T3453 |
arg2Of |
g,at |
R2966 |
T3455 |
T3454 |
arg1Of |
g,"12,000" |
R2967 |
T3458 |
T3456 |
arg2Of |
min,for |
R2968 |
T3458 |
T3457 |
arg1Of |
min,15 |
R2969 |
T3458 |
T3459 |
arg1Of |
min,at |
R2970 |
T3460 |
T3459 |
arg2Of |
4°C,at |
R2971 |
T3462 |
T3461 |
arg1Of |
concentrations,Protein |
R2972 |
T3462 |
T3463 |
arg1Of |
concentrations,were |
R2973 |
T3462 |
T3464 |
arg2Of |
concentrations,estimated |
R2974 |
T3464 |
T3463 |
arg2Of |
estimated,were |
R2975 |
T3470 |
T3464 |
arg1Of |
Reagent,estimated |
R2976 |
T3470 |
T3465 |
arg2Of |
Reagent,by |
R2977 |
T3470 |
T3466 |
arg1Of |
Reagent,Coomassie |
R2978 |
T3470 |
T3467 |
arg1Of |
Reagent,Plus |
R2979 |
T3470 |
T3468 |
arg1Of |
Reagent,Protein |
R2980 |
T3470 |
T3469 |
arg1Of |
Reagent,Assay |
R2981 |
T3470 |
T3471 |
arg1Of |
Reagent,( |
R2982 |
T3472 |
T3473 |
arg1Of |
Pierce,"," |
R2983 |
T3473 |
T3471 |
arg2Of |
",",( |
R2984 |
T3473 |
T3475 |
arg1Of |
",","," |
R2985 |
T3474 |
T3473 |
arg2Of |
IL,"," |
R2986 |
T3476 |
T3475 |
arg2Of |
USA,"," |
R2987 |
T3477 |
T3471 |
arg3Of |
),( |
R2988 |
T3479 |
T3478 |
arg1Of |
micrograms,Fifty |
R2989 |
T3479 |
T3480 |
arg1Of |
micrograms,of |
R2990 |
T3479 |
T3486 |
arg1Of |
micrograms,were |
R2991 |
T3479 |
T3487 |
arg1Of |
micrograms,heat-denatured |
R2992 |
T3479 |
T3506 |
arg1Of |
micrograms,electroblotted |
R2993 |
T3485 |
T3480 |
arg2Of |
extracts,of |
R2994 |
T3485 |
T3481 |
arg1Of |
extracts,the |
R2995 |
T3485 |
T3482 |
arg1Of |
extracts,resulting |
R2996 |
T3485 |
T3483 |
arg1Of |
extracts,cytosolic |
R2997 |
T3485 |
T3484 |
arg1Of |
extracts,protein |
R2998 |
T3486 |
T3488 |
arg1Of |
were,in |
R2999 |
T3486 |
T3505 |
arg1Of |
were,and |
R3000 |
T3487 |
T3486 |
arg2Of |
heat-denatured,were |
R3001 |
T3492 |
T3488 |
arg2Of |
buffer,in |
R3002 |
T3492 |
T3489 |
arg1Of |
buffer,Laemmli |
R3003 |
T3492 |
T3490 |
arg1Of |
buffer,sample |
R3004 |
T3492 |
T3491 |
arg1Of |
buffer,loading |
R3005 |
T3492 |
T3493 |
arg1Of |
buffer,"," |
R3006 |
T3492 |
T3494 |
arg2Of |
buffer,separated |
R3007 |
T3496 |
T3497 |
arg1Of |
10,% |
R3008 |
T3503 |
T3494 |
arg1Of |
electrophoresis,separated |
R3009 |
T3503 |
T3495 |
arg2Of |
electrophoresis,by |
R3010 |
T3503 |
T3496 |
arg1Of |
electrophoresis,10 |
R3011 |
T3503 |
T3498 |
arg1Of |
electrophoresis,sodium |
R3012 |
T3503 |
T3499 |
arg1Of |
electrophoresis,dodecyl |
R3013 |
T3503 |
T3500 |
arg1Of |
electrophoresis,sulfate |
R3014 |
T3503 |
T3501 |
arg1Of |
electrophoresis,polyacrylamide |
R3015 |
T3503 |
T3502 |
arg1Of |
electrophoresis,gel |
R3016 |
T3505 |
T3504 |
arg1Of |
and,"," |
R3017 |
T3506 |
T3505 |
arg2Of |
electroblotted,and |
R3018 |
T3506 |
T3507 |
arg1Of |
electroblotted,onto |
R3019 |
T3510 |
T3507 |
arg2Of |
membrane,onto |
R3020 |
T3510 |
T3508 |
arg1Of |
membrane,a |
R3021 |
T3510 |
T3509 |
arg1Of |
membrane,nitrocellulose |
R3022 |
T3512 |
T3511 |
arg2Of |
immunoblotting,For |
R3023 |
T3514 |
T3515 |
arg1Of |
membranes,were |
R3024 |
T3514 |
T3516 |
arg2Of |
membranes,blocked |
R3025 |
T3516 |
T3511 |
arg1Of |
blocked,For |
R3026 |
T3516 |
T3513 |
arg1Of |
blocked,"," |
R3027 |
T3516 |
T3515 |
arg2Of |
blocked,were |
R3028 |
T3516 |
T3517 |
arg1Of |
blocked,with |
R3029 |
T3516 |
T3526 |
arg1Of |
blocked,overnight |
R3030 |
T3516 |
T3527 |
arg1Of |
blocked,at |
R3031 |
T3516 |
T3530 |
arg1Of |
blocked,and |
R3032 |
T3518 |
T3519 |
arg1Of |
5,% |
R3033 |
T3522 |
T3517 |
arg2Of |
milk,with |
R3034 |
T3522 |
T3518 |
arg1Of |
milk,5 |
R3035 |
T3522 |
T3520 |
arg1Of |
milk,nonfat |
R3036 |
T3522 |
T3521 |
arg1Of |
milk,dry |
R3037 |
T3522 |
T3523 |
arg1Of |
milk,in |
R3038 |
T3525 |
T3523 |
arg2Of |
buffer,in |
R3039 |
T3525 |
T3524 |
arg1Of |
buffer,saline |
R3040 |
T3528 |
T3527 |
arg2Of |
4°C,at |
R3041 |
T3530 |
T3529 |
arg1Of |
and,"," |
R3042 |
T3530 |
T3536 |
arg1Of |
and,: |
R3043 |
T3530 |
T3537 |
arg1Of |
and,anti-β-actin |
R3044 |
T3530 |
T3554 |
arg1Of |
and,metallopeptidase |
R3045 |
T3533 |
T3531 |
arg1Of |
antibodies,the |
R3046 |
T3533 |
T3532 |
arg1Of |
antibodies,following |
R3047 |
T3533 |
T3534 |
arg1Of |
antibodies,were |
R3048 |
T3533 |
T3535 |
arg2Of |
antibodies,used |
R3049 |
T3535 |
T3530 |
arg2Of |
used,and |
R3050 |
T3535 |
T3534 |
arg2Of |
used,were |
R3051 |
T3537 |
T3538 |
arg1Of |
anti-β-actin,( |
R3052 |
T3537 |
T3552 |
arg1Of |
anti-β-actin,and |
R3053 |
T3539 |
T3540 |
arg1Of |
sc-130657,"," |
R3054 |
T3539 |
T3544 |
arg1Of |
sc-130657,"," |
R3055 |
T3543 |
T3540 |
arg2Of |
Biotechnology,"," |
R3056 |
T3543 |
T3541 |
arg1Of |
Biotechnology,Santa |
R3057 |
T3543 |
T3542 |
arg1Of |
Biotechnology,Cruz |
R3058 |
T3544 |
T3538 |
arg2Of |
",",( |
R3059 |
T3545 |
T3546 |
arg1Of |
CA,"," |
R3060 |
T3546 |
T3548 |
arg1Of |
",","," |
R3061 |
T3547 |
T3546 |
arg2Of |
USA,"," |
R3062 |
T3549 |
T3548 |
arg2Of |
43,"," |
R3063 |
T3550 |
T3544 |
arg2Of |
kDa,"," |
R3064 |
T3550 |
T3545 |
arg1Of |
kDa,CA |
R3065 |
T3550 |
T3547 |
arg1Of |
kDa,USA |
R3066 |
T3550 |
T3549 |
arg1Of |
kDa,43 |
R3067 |
T3551 |
T3538 |
arg3Of |
),( |
R3068 |
T3552 |
T3559 |
arg1Of |
and,( |
R3069 |
T3554 |
T3552 |
arg2Of |
metallopeptidase,and |
R3070 |
T3554 |
T3553 |
arg1Of |
metallopeptidase,anti-matrix |
R3071 |
T3554 |
T3555 |
arg1Of |
metallopeptidase,9 |
R3072 |
T3554 |
T3556 |
arg1Of |
metallopeptidase,( |
R3073 |
T3557 |
T3556 |
arg2Of |
MMP9,( |
R3074 |
T3558 |
T3556 |
arg3Of |
),( |
R3075 |
T3560 |
T3561 |
arg1Of |
sc-6841,"," |
R3076 |
T3560 |
T3565 |
arg1Of |
sc-6841,"," |
R3077 |
T3564 |
T3561 |
arg2Of |
Biotechnology,"," |
R3078 |
T3564 |
T3562 |
arg1Of |
Biotechnology,Santa |
R3079 |
T3564 |
T3563 |
arg1Of |
Biotechnology,Cruz |
R3080 |
T3565 |
T3559 |
arg2Of |
",",( |
R3081 |
T3566 |
T3567 |
arg1Of |
CA,"," |
R3082 |
T3567 |
T3569 |
arg1Of |
",","," |
R3083 |
T3568 |
T3567 |
arg2Of |
USA,"," |
R3084 |
T3570 |
T3569 |
arg2Of |
92,"," |
R3085 |
T3571 |
T3565 |
arg2Of |
kDa,"," |
R3086 |
T3571 |
T3566 |
arg1Of |
kDa,CA |
R3087 |
T3571 |
T3568 |
arg1Of |
kDa,USA |
R3088 |
T3571 |
T3570 |
arg1Of |
kDa,92 |
R3089 |
T3572 |
T3559 |
arg3Of |
),( |
R3090 |
T3575 |
T3573 |
arg1Of |
antibody,Each |
R3091 |
T3575 |
T3574 |
arg1Of |
antibody,primary |
R3092 |
T3575 |
T3576 |
arg1Of |
antibody,was |
R3093 |
T3575 |
T3577 |
arg2Of |
antibody,diluted |
R3094 |
T3575 |
T3584 |
arg1Of |
antibody,added |
R3095 |
T3577 |
T3576 |
arg2Of |
diluted,was |
R3096 |
T3577 |
T3578 |
arg1Of |
diluted,appropriately |
R3097 |
T3577 |
T3579 |
arg1Of |
diluted,in |
R3098 |
T3577 |
T3582 |
arg1Of |
diluted,and |
R3099 |
T3580 |
T3579 |
arg2Of |
blocking,in |
R3100 |
T3581 |
T3580 |
arg2Of |
buffer,blocking |
R3101 |
T3584 |
T3582 |
arg2Of |
added,and |
R3102 |
T3584 |
T3583 |
arg1Of |
added,then |
R3103 |
T3584 |
T3585 |
arg1Of |
added,to |
R3104 |
T3584 |
T3588 |
arg1Of |
added,for |
R3105 |
T3587 |
T3585 |
arg2Of |
blots,to |
R3106 |
T3587 |
T3586 |
arg1Of |
blots,the |
R3107 |
T3590 |
T3588 |
arg2Of |
h,for |
R3108 |
T3590 |
T3589 |
arg1Of |
h,1 |
R3109 |
T3590 |
T3591 |
arg1Of |
h,at |
R3110 |
T3593 |
T3591 |
arg2Of |
temperature,at |
R3111 |
T3593 |
T3592 |
arg1Of |
temperature,room |
R3112 |
T3595 |
T3594 |
arg1Of |
blots,The |
R3113 |
T3595 |
T3596 |
arg1Of |
blots,were |
R3114 |
T3595 |
T3597 |
arg2Of |
blots,washed |
R3115 |
T3595 |
T3605 |
arg2Of |
blots,covered |
R3116 |
T3597 |
T3599 |
arg1Of |
washed,times |
R3117 |
T3597 |
T3600 |
arg1Of |
washed,in |
R3118 |
T3597 |
T3604 |
arg1Of |
washed,and |
R3119 |
T3599 |
T3598 |
arg1Of |
times,three |
R3120 |
T3603 |
T3600 |
arg2Of |
buffer,in |
R3121 |
T3603 |
T3601 |
arg1Of |
buffer,the |
R3122 |
T3603 |
T3602 |
arg1Of |
buffer,washing |
R3123 |
T3604 |
T3596 |
arg2Of |
and,were |
R3124 |
T3605 |
T3604 |
arg2Of |
covered,and |
R3125 |
T3605 |
T3606 |
arg1Of |
covered,with |
R3126 |
T3605 |
T3612 |
arg1Of |
covered,at |
R3127 |
T3611 |
T3606 |
arg2Of |
antibody,with |
R3128 |
T3611 |
T3607 |
arg1Of |
antibody,the |
R3129 |
T3611 |
T3608 |
arg1Of |
antibody,horseradish |
R3130 |
T3611 |
T3609 |
arg1Of |
antibody,peroxidase-linked |
R3131 |
T3611 |
T3610 |
arg1Of |
antibody,secondary |
R3132 |
T3614 |
T3612 |
arg2Of |
1,at |
R3133 |
T3614 |
T3613 |
arg1Of |
1,a |
R3134 |
T3614 |
T3615 |
arg1Of |
1,: |
R3135 |
T3617 |
T3616 |
arg1Of |
dilution,2000 |
R3136 |
T3617 |
T3618 |
arg1Of |
dilution,for |
R3137 |
T3620 |
T3618 |
arg2Of |
h,for |
R3138 |
T3620 |
T3619 |
arg1Of |
h,1 |
R3139 |
T3621 |
T3622 |
arg1Of |
Blots,were |
R3140 |
T3621 |
T3623 |
arg2Of |
Blots,incubated |
R3141 |
T3621 |
T3641 |
arg2Of |
Blots,exposed |
R3142 |
T3623 |
T3624 |
arg1Of |
incubated,with |
R3143 |
T3623 |
T3640 |
arg1Of |
incubated,and |
R3144 |
T3628 |
T3627 |
arg2Of |
ECL,( |
R3145 |
T3629 |
T3627 |
arg3Of |
),( |
R3146 |
T3631 |
T3624 |
arg2Of |
system,with |
R3147 |
T3631 |
T3625 |
arg2Of |
system,enhanced |
R3148 |
T3631 |
T3626 |
arg1Of |
system,chemiluminescence |
R3149 |
T3631 |
T3627 |
arg1Of |
system,( |
R3150 |
T3631 |
T3630 |
arg1Of |
system,detection |
R3151 |
T3631 |
T3632 |
arg1Of |
system,( |
R3152 |
T3634 |
T3632 |
arg2Of |
Biosciences,( |
R3153 |
T3634 |
T3633 |
arg1Of |
Biosciences,Amersham |
R3154 |
T3634 |
T3635 |
arg1Of |
Biosciences,"," |
R3155 |
T3636 |
T3635 |
arg2Of |
Bucks,"," |
R3156 |
T3636 |
T3637 |
arg1Of |
Bucks,"," |
R3157 |
T3638 |
T3637 |
arg2Of |
UK,"," |
R3158 |
T3639 |
T3632 |
arg3Of |
),( |
R3159 |
T3640 |
T3622 |
arg2Of |
and,were |
R3160 |
T3641 |
T3640 |
arg2Of |
exposed,and |
R3161 |
T3641 |
T3642 |
arg1Of |
exposed,to |
R3162 |
T3644 |
T3642 |
arg2Of |
film,to |
R3163 |
T3644 |
T3643 |
arg1Of |
film,radiographic |
R3164 |
T3644 |
T3645 |
arg1Of |
film,( |
R3165 |
T3647 |
T3645 |
arg2Of |
Hyperfilm,( |
R3166 |
T3647 |
T3646 |
arg1Of |
Hyperfilm,Fuji |
R3167 |
T3647 |
T3648 |
arg1Of |
Hyperfilm,"," |
R3168 |
T3649 |
T3648 |
arg2Of |
Tokyo,"," |
R3169 |
T3649 |
T3650 |
arg1Of |
Tokyo,"," |
R3170 |
T3651 |
T3650 |
arg2Of |
Japan,"," |
R3171 |
T3652 |
T3645 |
arg3Of |
),( |
R3172 |
T3653 |
T3654 |
arg1Of |
ImageJ,was |
R3173 |
T3653 |
T3655 |
arg2Of |
ImageJ,used |
R3174 |
T3655 |
T3654 |
arg2Of |
used,was |
R3175 |
T3657 |
T3655 |
arg3Of |
analyze,used |
R3176 |
T3657 |
T3656 |
arg1Of |
analyze,to |
R3177 |
T3659 |
T3657 |
arg2Of |
intensity,analyze |
R3178 |
T3659 |
T3658 |
arg1Of |
intensity,the |
R3179 |
T3659 |
T3660 |
arg1Of |
intensity,of |
R3180 |
T3662 |
T3660 |
arg2Of |
blots,of |
R3181 |
T3662 |
T3661 |
arg1Of |
blots,the |
R3182 |
T3664 |
T3663 |
arg1Of |
level,The |
R3183 |
T3664 |
T3665 |
arg1Of |
level,of |
R3184 |
T3664 |
T3667 |
arg1Of |
level,was |
R3185 |
T3664 |
T3668 |
arg2Of |
level,used |
R3186 |
T3666 |
T3665 |
arg2Of |
β-actin,of |
R3187 |
T3668 |
T3667 |
arg2Of |
used,was |
R3188 |
T3668 |
T3669 |
arg1Of |
used,as |
R3189 |
T3671 |
T3669 |
arg2Of |
standard,as |
R3190 |
T3671 |
T3670 |
arg1Of |
standard,internal |