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PMC:1310901 / 6810-8716 JSONTXT 17 Projects

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Id Subject Object Predicate Lexical cue
T3450 0-10 NN denotes Expression
T3451 11-19 NN denotes analysis
T3452 20-22 TO denotes To
T3453 23-30 VB denotes analyze
T3454 31-34 DT denotes the
T3455 35-40 NN denotes IRF-4
T3456 41-56 JJ denotes transcriptional
T3457 57-62 NN denotes level
T3458 62-63 -COMMA- denotes ,
T3459 64-67 NN denotes RNA
T3460 68-71 VB denotes was
T3461 72-81 VB denotes extracted
T3462 82-86 IN denotes from
T3463 87-92 NN denotes cells
T3464 93-98 VB denotes using
T3465 99-102 DT denotes the
T3466 103-113 JJ denotes commercial
T3467 114-124 NN denotes RNAzol-kit
T3468 125-126 -LRB- denotes (
T3469 126-132 NNP denotes Paesel
T3470 132-133 -COMMA- denotes ,
T3471 134-143 NNP denotes Frankfurt
T3472 143-144 -COMMA- denotes ,
T3473 145-152 NNP denotes Germany
T3474 152-153 -RRB- denotes )
T3475 155-157 DT denotes An
T3476 158-165 NN denotes aliquot
T3477 166-168 IN denotes of
T3478 169-170 CD denotes 1
T3479 171-173 NN denotes μg
T3480 174-179 JJ denotes total
T3481 180-183 NN denotes RNA
T3482 184-187 VB denotes was
T3483 188-192 VB denotes used
T3484 193-196 IN denotes for
T3485 197-201 NN denotes cDNA
T3486 202-211 NN denotes synthesis
T3487 212-214 IN denotes as
T3488 215-224 VB denotes described
T3489 225-235 RB denotes previously
T3490 236-237 -LRB- denotes (
T3491 237-239 CD denotes 27
T3492 239-240 -RRB- denotes )
T3493 242-245 NN denotes RNA
T3494 246-256 NN denotes expression
T3495 257-265 NN denotes analysis
T3496 266-269 IN denotes for
T3497 270-275 NN denotes IRF-4
T3498 276-279 CC denotes and
T3499 280-283 DT denotes the
T3500 284-293 NN denotes reference
T3501 294-298 NN denotes gene
T3502 299-306 NN denotes β-actin
T3503 307-310 VB denotes was
T3504 311-318 VB denotes carried
T3505 319-322 RP denotes out
T3506 323-325 IN denotes by
T3507 326-343 JJ denotes semi-quantitative
T3508 344-347 NN denotes PCR
T3509 348-350 IN denotes as
T3510 351-360 VB denotes described
T3511 361-371 RB denotes previously
T3512 372-373 -LRB- denotes (
T3513 373-377 CD denotes 3,27
T3514 377-378 -RRB- denotes )
T3515 380-383 NN denotes PCR
T3516 384-392 NN denotes products
T3517 393-397 VB denotes were
T3518 398-406 VB denotes verified
T3519 407-409 IN denotes by
T3520 410-419 VB denotes automated
T3521 420-430 NN denotes sequencing
T3522 432-435 NN denotes PCR
T3523 436-443 NN denotes primers
T3524 444-447 CC denotes and
T3525 448-458 NN denotes conditions
T3526 459-462 IN denotes for
T3527 463-473 NN denotes expression
T3528 474-482 NN denotes analysis
T3529 483-485 IN denotes of
T3530 486-490 NN denotes DNMT
T3531 491-493 CC denotes or
T3532 494-497 NN denotes MBP
T3533 498-499 -LRB- denotes (
T3534 499-504 NN denotes DNMT1
T3535 505-511 NN denotes DNMT3A
T3536 511-512 -COMMA- denotes ,
T3537 513-519 NN denotes DNMT3B
T3538 519-520 -COMMA- denotes ,
T3539 521-525 NN denotes MeCP
T3540 525-526 -COMMA- denotes ,
T3541 527-531 NN denotes MBD1
T3542 531-532 -COMMA- denotes ,
T3543 533-537 NN denotes MBD2
T3544 538-541 CC denotes and
T3545 542-546 NN denotes MBD4
T3546 546-547 -RRB- denotes )
T3547 548-552 VB denotes were
T3548 553-562 VB denotes published
T3549 563-572 RB denotes elsewhere
T3550 573-574 -LRB- denotes (
T3551 574-576 CD denotes 28
T3552 576-577 -RRB- denotes )
T3553 579-582 IN denotes For
T3554 583-591 NN denotes analysis
T3555 592-594 IN denotes of
T3556 595-600 NN denotes IRF-4
T3557 601-608 NN denotes protein
T3558 609-619 NN denotes expression
T3559 619-620 -COMMA- denotes ,
T3560 621-622 DT denotes a
T3561 623-631 JJ denotes standard
T3562 632-646 NN denotes immunoblotting
T3563 647-652 NN denotes assay
T3564 653-656 VB denotes was
T3565 657-666 VB denotes performed
T3566 667-669 IN denotes as
T3567 670-679 VB denotes described
T3568 680-690 RB denotes previously
T3569 691-692 -LRB- denotes (
T3570 692-694 CD denotes 29
T3571 694-695 -RRB- denotes )
T3572 697-704 RB denotes Briefly
T3573 704-705 -COMMA- denotes ,
T3574 706-713 NN denotes protein
T3575 714-721 NN denotes lysates
T3576 722-726 VB denotes were
T3577 727-736 VB denotes generated
T3578 737-739 IN denotes by
T3579 740-750 VB denotes incubating
T3580 751-752 CD denotes 1
T3581 753-754 NN denotes ×
T3582 755-758 CD denotes 106
T3583 759-764 NN denotes cells
T3584 765-767 IN denotes in
T3585 768-771 CD denotes 100
T3586 772-774 NN denotes µl
T3587 775-779 NN denotes RIPA
T3588 780-786 NN denotes buffer
T3589 787-788 -LRB- denotes (
T3590 788-789 CD denotes 1
T3591 789-790 NN denotes %
T3592 791-796 NN denotes NP-40
T3593 796-797 -COMMA- denotes ,
T3594 798-801 CD denotes 0.5
T3595 801-802 NN denotes %
T3596 803-822 NN denotes sodiumdesoxycholate
T3597 822-823 -COMMA- denotes ,
T3598 824-827 CD denotes 0.1
T3599 827-828 NN denotes %
T3600 829-832 NN denotes SDS
T3601 832-833 -COMMA- denotes ,
T3602 834-837 CD denotes 100
T3603 838-843 NN denotes µg/ml
T3604 844-864 NN denotes phenylmethylsulfonyl
T3605 865-873 NN denotes fluoride
T3606 873-874 -COMMA- denotes ,
T3607 875-877 CD denotes 10
T3608 878-883 NN denotes µl/ml
T3609 884-907 NN denotes protease-inhibitory-mix
T3610 907-908 -COMMA- denotes ,
T3611 909-910 CD denotes 1
T3612 911-918 NN denotes µmol/ml
T3613 919-938 NN denotes sodiumorthovanadate
T3614 939-941 IN denotes in
T3615 942-960 JJ denotes phosphate-buffered
T3616 961-967 NN denotes saline
T3617 967-968 -RRB- denotes )
T3618 969-972 IN denotes for
T3619 973-975 CD denotes 30
T3620 976-979 NN denotes min
T3621 980-982 IN denotes on
T3622 983-986 NN denotes ice
T3623 988-993 IN denotes After
T3624 994-1008 NN denotes centrifugation
T3625 1008-1009 -COMMA- denotes ,
T3626 1010-1017 NN denotes protein
T3627 1018-1031 NN denotes concentration
T3628 1032-1034 IN denotes of
T3629 1035-1038 DT denotes the
T3630 1039-1050 NN denotes supernatant
T3631 1051-1054 VB denotes was
T3632 1055-1065 VB denotes determined
T3633 1066-1068 IN denotes by
T3634 1069-1079 NN denotes BCA-method
T3635 1080-1081 -LRB- denotes (
T3636 1081-1087 NNP denotes Pierce
T3637 1087-1088 -COMMA- denotes ,
T3638 1089-1097 NNP denotes Rockford
T3639 1097-1098 -COMMA- denotes ,
T3640 1099-1101 NN denotes IL
T3641 1101-1102 -RRB- denotes )
T3642 1103-1105 IN denotes as
T3643 1106-1117 VB denotes recommended
T3644 1119-1126 NN denotes Protein
T3645 1127-1134 NN denotes lysates
T3646 1135-1136 -LRB- denotes (
T3647 1136-1142 CD denotes 70–100
T3648 1143-1145 NN denotes µg
T3649 1145-1146 -RRB- denotes )
T3650 1147-1151 VB denotes were
T3651 1152-1167 VB denotes electrophoresed
T3652 1168-1170 IN denotes on
T3653 1171-1185 NN denotes polyacrylamide
T3654 1186-1190 NN denotes gels
T3655 1191-1194 CC denotes and
T3656 1195-1206 VB denotes transferred
T3657 1207-1209 TO denotes to
T3658 1210-1211 DT denotes a
T3659 1212-1225 NN denotes PVDF-membrane
T3660 1226-1227 -LRB- denotes (
T3661 1227-1236 NNP denotes Immobilon
T3662 1237-1238 NNP denotes P
T3663 1238-1239 -COMMA- denotes ,
T3664 1240-1244 CD denotes 0.45
T3665 1245-1247 NN denotes µm
T3666 1247-1248 -COLON- denotes ;
T3667 1249-1258 NNP denotes Millipore
T3668 1258-1259 -COMMA- denotes ,
T3669 1260-1268 NNP denotes Eschborn
T3670 1268-1269 -COMMA- denotes ,
T3671 1270-1277 NNP denotes Germany
T3672 1277-1278 -RRB- denotes )
T3673 1280-1289 NN denotes Membranes
T3674 1290-1294 VB denotes were
T3675 1295-1302 VB denotes blocked
T3676 1303-1307 IN denotes with
T3677 1308-1311 CD denotes 2.5
T3678 1311-1312 NN denotes %
T3679 1313-1321 VB denotes blocking
T3680 1322-1329 NN denotes reagent
T3681 1330-1331 -LRB- denotes (
T3682 1331-1341 NNP denotes Boehringer
T3683 1342-1350 NNP denotes Mannheim
T3684 1350-1351 -COMMA- denotes ,
T3685 1352-1359 NNP denotes Germany
T3686 1359-1360 -RRB- denotes )
T3687 1361-1363 IN denotes in
T3688 1364-1368 NN denotes TBST
T3689 1369-1375 NN denotes buffer
T3690 1376-1377 -LRB- denotes (
T3691 1377-1381 CD denotes 4.44
T3692 1382-1385 NN denotes g/l
T3693 1386-1394 NN denotes Tris–HCL
T3694 1394-1395 -COMMA- denotes ,
T3695 1396-1400 CD denotes 2.65
T3696 1401-1404 NN denotes g/l
T3697 1405-1411 NN denotes TrisOH
T3698 1411-1412 -COMMA- denotes ,
T3699 1413-1417 CD denotes 8.07
T3700 1418-1421 NN denotes g/l
T3701 1422-1426 NN denotes NaCl
T3702 1426-1427 -COMMA- denotes ,
T3703 1428-1431 CD denotes 0.2
T3704 1432-1435 NN denotes g/l
T3705 1436-1439 NN denotes KCl
T3706 1440-1443 CC denotes and
T3707 1444-1447 CD denotes 500
T3708 1448-1452 VB denotes µl/l
T3709 1453-1461 CD denotes Tween-20
T3710 1462-1464 IN denotes in
T3711 1465-1468 NN denotes H2O
T3712 1468-1469 -RRB- denotes )
T3713 1470-1473 CC denotes and
T3714 1474-1486 RB denotes subsequently
T3715 1487-1496 VB denotes incubated
T3716 1497-1501 IN denotes with
T3717 1502-1509 JJ denotes primary
T3718 1510-1518 NN denotes antibody
T3719 1519-1521 IN denotes as
T3720 1522-1531 VB denotes indicated
T3721 1532-1535 CC denotes and
T3722 1536-1547 JJ denotes horseradish
T3723 1548-1569 JJ denotes peroxidase-conjugated
T3724 1570-1579 JJ denotes secondary
T3725 1580-1588 NN denotes antibody
T3726 1588-1589 -COMMA- denotes ,
T3727 1590-1600 JJ denotes anti-mouse
T3728 1601-1603 CC denotes or
T3729 1604-1613 JJ denotes anti-goat
T3730 1614-1617 NN denotes IgG
T3731 1618-1619 -LRB- denotes (
T3732 1619-1623 NNP denotes DAKO
T3733 1623-1624 -COMMA- denotes ,
T3734 1625-1632 NNP denotes Hamburg
T3735 1632-1633 -COMMA- denotes ,
T3736 1634-1641 NNP denotes Germany
T3737 1641-1642 -RRB- denotes )
T3738 1642-1643 -COMMA- denotes ,
T3739 1644-1656 RB denotes respectively
T3740 1658-1661 DT denotes The
T3741 1662-1671 NN denotes membranes
T3742 1672-1676 VB denotes were
T3743 1677-1681 RB denotes then
T3744 1682-1691 VB denotes developed
T3745 1692-1696 IN denotes with
T3746 1697-1699 DT denotes an
T3747 1700-1703 NN denotes ECL
T3748 1704-1713 NN denotes detection
T3749 1714-1717 NN denotes kit
T3750 1718-1719 -LRB- denotes (
T3751 1719-1727 NNP denotes Amersham
T3752 1728-1737 NNP denotes Pharmacia
T3753 1738-1745 NNP denotes Biotech
T3754 1745-1746 -COMMA- denotes ,
T3755 1747-1755 NNP denotes Freiburg
T3756 1755-1756 -COMMA- denotes ,
T3757 1757-1764 NNP denotes Germany
T3758 1764-1765 -RRB- denotes )
T3759 1767-1770 DT denotes The
T3760 1771-1778 JJ denotes primary
T3761 1779-1789 NN denotes antibodies
T3762 1790-1794 VB denotes were
T3763 1795-1799 NN denotes goat
T3764 1800-1816 NN denotes anti-IRF-4/ICSAT
T3765 1817-1818 -LRB- denotes (
T3766 1818-1822 NN denotes M-17
T3767 1822-1823 -RRB- denotes )
T3768 1824-1825 -LRB- denotes (
T3769 1825-1830 NNP denotes Santa
T3770 1831-1835 NNP denotes Cruz
T3771 1836-1849 NNP denotes Biotechnology
T3772 1849-1850 -COMMA- denotes ,
T3773 1851-1856 NNP denotes Santa
T3774 1857-1861 NNP denotes Cruz
T3775 1861-1862 -COMMA- denotes ,
T3776 1863-1865 NN denotes CA
T3777 1865-1866 -RRB- denotes )
T3778 1867-1870 CC denotes and
T3779 1871-1876 NN denotes mouse
T3780 1877-1889 JJ denotes anti-β-actin
T3781 1890-1891 -LRB- denotes (
T3782 1891-1896 NN denotes AC-74
T3783 1896-1897 -RRB- denotes )
T3784 1898-1899 -LRB- denotes (
T3785 1899-1904 NN denotes Sigma
T3786 1904-1905 -RRB- denotes )
R2749 T3451 T3450 arg1Of analysis,Expression
R2750 T3453 T3452 arg1Of analyze,To
R2751 T3457 T3453 arg2Of level,analyze
R2752 T3457 T3454 arg1Of level,the
R2753 T3457 T3455 arg1Of level,IRF-4
R2754 T3457 T3456 arg1Of level,transcriptional
R2755 T3459 T3453 arg1Of RNA,analyze
R2756 T3459 T3460 arg1Of RNA,was
R2757 T3459 T3461 arg2Of RNA,extracted
R2758 T3461 T3452 modOf extracted,To
R2759 T3461 T3458 arg1Of extracted,","
R2760 T3461 T3460 arg2Of extracted,was
R2761 T3461 T3462 arg1Of extracted,from
R2762 T3463 T3462 arg2Of cells,from
R2763 T3463 T3464 arg1Of cells,using
R2764 T3467 T3464 arg2Of RNAzol-kit,using
R2765 T3467 T3465 arg1Of RNAzol-kit,the
R2766 T3467 T3466 arg1Of RNAzol-kit,commercial
R2767 T3467 T3468 arg1Of RNAzol-kit,(
R2768 T3469 T3470 arg1Of Paesel,","
R2769 T3470 T3468 arg2Of ",",(
R2770 T3470 T3472 arg1Of ",",","
R2771 T3471 T3470 arg2Of Frankfurt,","
R2772 T3473 T3472 arg2Of Germany,","
R2773 T3474 T3468 arg3Of ),(
R2774 T3476 T3475 arg1Of aliquot,An
R2775 T3476 T3477 arg1Of aliquot,of
R2776 T3476 T3482 arg1Of aliquot,was
R2777 T3476 T3483 arg2Of aliquot,used
R2778 T3481 T3477 arg2Of RNA,of
R2779 T3481 T3478 arg1Of RNA,1
R2780 T3481 T3479 arg1Of RNA,μg
R2781 T3481 T3480 arg1Of RNA,total
R2782 T3483 T3482 arg2Of used,was
R2783 T3483 T3484 arg1Of used,for
R2784 T3486 T3484 arg2Of synthesis,for
R2785 T3486 T3485 arg1Of synthesis,cDNA
R2786 T3487 T3483 arg3Of as,used
R2787 T3488 T3487 arg2Of described,as
R2788 T3488 T3489 arg1Of described,previously
R2789 T3489 T3490 arg1Of previously,(
R2790 T3491 T3490 arg2Of 27,(
R2791 T3492 T3490 arg3Of ),(
R2792 T3495 T3493 arg1Of analysis,RNA
R2793 T3495 T3494 arg1Of analysis,expression
R2794 T3495 T3496 arg1Of analysis,for
R2795 T3495 T3498 arg1Of analysis,and
R2796 T3497 T3496 arg2Of IRF-4,for
R2797 T3498 T3503 arg1Of and,was
R2798 T3498 T3504 arg2Of and,carried
R2799 T3502 T3498 arg2Of β-actin,and
R2800 T3502 T3499 arg1Of β-actin,the
R2801 T3502 T3500 arg1Of β-actin,reference
R2802 T3502 T3501 arg1Of β-actin,gene
R2803 T3504 T3503 arg2Of carried,was
R2804 T3504 T3505 arg1Of carried,out
R2805 T3504 T3509 arg1Of carried,as
R2806 T3508 T3504 arg1Of PCR,carried
R2807 T3508 T3506 arg2Of PCR,by
R2808 T3508 T3507 arg1Of PCR,semi-quantitative
R2809 T3510 T3509 arg2Of described,as
R2810 T3510 T3511 arg1Of described,previously
R2811 T3511 T3512 arg1Of previously,(
R2812 T3513 T3512 arg2Of "3,27",(
R2813 T3514 T3512 arg3Of ),(
R2814 T3516 T3515 arg1Of products,PCR
R2815 T3516 T3517 arg1Of products,were
R2816 T3516 T3518 arg2Of products,verified
R2817 T3518 T3517 arg2Of verified,were
R2818 T3521 T3518 arg1Of sequencing,verified
R2819 T3521 T3519 arg2Of sequencing,by
R2820 T3521 T3520 arg2Of sequencing,automated
R2821 T3523 T3522 arg1Of primers,PCR
R2822 T3523 T3524 arg1Of primers,and
R2823 T3524 T3526 arg1Of and,for
R2824 T3524 T3547 arg1Of and,were
R2825 T3524 T3548 arg2Of and,published
R2826 T3525 T3524 arg2Of conditions,and
R2827 T3528 T3526 arg2Of analysis,for
R2828 T3528 T3527 arg1Of analysis,expression
R2829 T3528 T3529 arg1Of analysis,of
R2830 T3530 T3531 arg1Of DNMT,or
R2831 T3531 T3529 arg2Of or,of
R2832 T3531 T3533 arg1Of or,(
R2833 T3532 T3531 arg2Of MBP,or
R2834 T3535 T3534 arg1Of DNMT3A,DNMT1
R2835 T3535 T3536 arg1Of DNMT3A,","
R2836 T3536 T3538 arg1Of ",",","
R2837 T3537 T3536 arg2Of DNMT3B,","
R2838 T3538 T3540 arg1Of ",",","
R2839 T3539 T3538 arg2Of MeCP,","
R2840 T3540 T3542 arg1Of ",",","
R2841 T3541 T3540 arg2Of MBD1,","
R2842 T3542 T3544 arg1Of ",",and
R2843 T3543 T3542 arg2Of MBD2,","
R2844 T3544 T3533 arg2Of and,(
R2845 T3545 T3544 arg2Of MBD4,and
R2846 T3546 T3533 arg3Of ),(
R2847 T3548 T3547 arg2Of published,were
R2848 T3548 T3549 arg1Of published,elsewhere
R2849 T3549 T3550 arg1Of elsewhere,(
R2850 T3551 T3550 arg2Of 28,(
R2851 T3552 T3550 arg3Of ),(
R2852 T3554 T3553 arg2Of analysis,For
R2853 T3554 T3555 arg1Of analysis,of
R2854 T3558 T3555 arg2Of expression,of
R2855 T3558 T3556 arg1Of expression,IRF-4
R2856 T3558 T3557 arg1Of expression,protein
R2857 T3563 T3560 arg1Of assay,a
R2858 T3563 T3561 arg1Of assay,standard
R2859 T3563 T3562 arg1Of assay,immunoblotting
R2860 T3563 T3564 arg1Of assay,was
R2861 T3563 T3565 arg2Of assay,performed
R2862 T3565 T3553 arg1Of performed,For
R2863 T3565 T3559 arg1Of performed,","
R2864 T3565 T3564 arg2Of performed,was
R2865 T3565 T3566 arg1Of performed,as
R2866 T3567 T3566 arg2Of described,as
R2867 T3567 T3568 arg1Of described,previously
R2868 T3568 T3569 arg1Of previously,(
R2869 T3570 T3569 arg2Of 29,(
R2870 T3571 T3569 arg3Of ),(
R2871 T3575 T3574 arg1Of lysates,protein
R2872 T3575 T3576 arg1Of lysates,were
R2873 T3575 T3577 arg2Of lysates,generated
R2874 T3577 T3572 arg1Of generated,Briefly
R2875 T3577 T3573 arg1Of generated,","
R2876 T3577 T3576 arg2Of generated,were
R2877 T3577 T3578 arg1Of generated,by
R2878 T3579 T3578 arg2Of incubating,by
R2879 T3579 T3618 arg1Of incubating,for
R2880 T3583 T3579 arg2Of cells,incubating
R2881 T3583 T3580 arg1Of cells,1
R2882 T3583 T3581 arg1Of cells,×
R2883 T3583 T3582 arg1Of cells,106
R2884 T3583 T3584 arg1Of cells,in
R2885 T3583 T3589 arg1Of cells,(
R2886 T3588 T3584 arg2Of buffer,in
R2887 T3588 T3585 arg1Of buffer,100
R2888 T3588 T3586 arg1Of buffer,µl
R2889 T3588 T3587 arg1Of buffer,RIPA
R2890 T3590 T3591 arg1Of 1,%
R2891 T3592 T3590 arg1Of NP-40,1
R2892 T3592 T3593 arg1Of NP-40,","
R2893 T3593 T3597 arg1Of ",",","
R2894 T3594 T3595 arg1Of 0.5,%
R2895 T3596 T3593 arg2Of sodiumdesoxycholate,","
R2896 T3596 T3594 arg1Of sodiumdesoxycholate,0.5
R2897 T3597 T3601 arg1Of ",",","
R2898 T3598 T3599 arg1Of 0.1,%
R2899 T3600 T3597 arg2Of SDS,","
R2900 T3600 T3598 arg1Of SDS,0.1
R2901 T3601 T3606 arg1Of ",",","
R2902 T3605 T3601 arg2Of fluoride,","
R2903 T3605 T3602 arg1Of fluoride,100
R2904 T3605 T3603 arg1Of fluoride,µg/ml
R2905 T3605 T3604 arg1Of fluoride,phenylmethylsulfonyl
R2906 T3606 T3610 arg1Of ",",","
R2907 T3609 T3606 arg2Of protease-inhibitory-mix,","
R2908 T3609 T3607 arg1Of protease-inhibitory-mix,10
R2909 T3609 T3608 arg1Of protease-inhibitory-mix,µl/ml
R2910 T3610 T3589 arg2Of ",",(
R2911 T3613 T3610 arg2Of sodiumorthovanadate,","
R2912 T3613 T3611 arg1Of sodiumorthovanadate,1
R2913 T3613 T3612 arg1Of sodiumorthovanadate,µmol/ml
R2914 T3613 T3614 arg1Of sodiumorthovanadate,in
R2915 T3616 T3614 arg2Of saline,in
R2916 T3616 T3615 arg1Of saline,phosphate-buffered
R2917 T3617 T3589 arg3Of ),(
R2918 T3620 T3618 arg2Of min,for
R2919 T3620 T3619 arg1Of min,30
R2920 T3620 T3621 arg1Of min,on
R2921 T3622 T3621 arg2Of ice,on
R2922 T3624 T3623 arg2Of centrifugation,After
R2923 T3627 T3626 arg1Of concentration,protein
R2924 T3627 T3628 arg1Of concentration,of
R2925 T3627 T3631 arg1Of concentration,was
R2926 T3627 T3632 arg2Of concentration,determined
R2927 T3630 T3628 arg2Of supernatant,of
R2928 T3630 T3629 arg1Of supernatant,the
R2929 T3632 T3623 arg1Of determined,After
R2930 T3632 T3625 arg1Of determined,","
R2931 T3632 T3631 arg2Of determined,was
R2932 T3632 T3635 arg1Of determined,(
R2933 T3632 T3642 arg1Of determined,as
R2934 T3634 T3632 arg1Of BCA-method,determined
R2935 T3634 T3633 arg2Of BCA-method,by
R2936 T3636 T3637 arg1Of Pierce,","
R2937 T3637 T3635 arg2Of ",",(
R2938 T3637 T3639 arg1Of ",",","
R2939 T3638 T3637 arg2Of Rockford,","
R2940 T3640 T3639 arg2Of IL,","
R2941 T3641 T3635 arg3Of ),(
R2942 T3643 T3642 arg2Of recommended,as
R2943 T3645 T3644 arg1Of lysates,Protein
R2944 T3645 T3646 arg1Of lysates,(
R2945 T3645 T3650 arg1Of lysates,were
R2946 T3645 T3651 arg2Of lysates,electrophoresed
R2947 T3645 T3656 arg2Of lysates,transferred
R2948 T3648 T3646 arg2Of µg,(
R2949 T3648 T3647 arg1Of µg,70–100
R2950 T3649 T3646 arg3Of ),(
R2951 T3651 T3652 arg1Of electrophoresed,on
R2952 T3651 T3655 arg1Of electrophoresed,and
R2953 T3654 T3652 arg2Of gels,on
R2954 T3654 T3653 arg1Of gels,polyacrylamide
R2955 T3655 T3650 arg2Of and,were
R2956 T3656 T3655 arg2Of transferred,and
R2957 T3656 T3657 arg1Of transferred,to
R2958 T3659 T3657 arg2Of PVDF-membrane,to
R2959 T3659 T3658 arg1Of PVDF-membrane,a
R2960 T3659 T3660 arg1Of PVDF-membrane,(
R2961 T3662 T3660 arg2Of P,(
R2962 T3662 T3661 arg1Of P,Immobilon
R2963 T3662 T3663 arg1Of P,","
R2964 T3662 T3666 arg1Of P,;
R2965 T3665 T3663 arg2Of µm,","
R2966 T3665 T3664 arg1Of µm,0.45
R2967 T3667 T3666 arg2Of Millipore,;
R2968 T3667 T3668 arg1Of Millipore,","
R2969 T3669 T3668 arg2Of Eschborn,","
R2970 T3669 T3670 arg1Of Eschborn,","
R2971 T3671 T3670 arg2Of Germany,","
R2972 T3672 T3660 arg3Of ),(
R2973 T3673 T3674 arg1Of Membranes,were
R2974 T3673 T3675 arg2Of Membranes,blocked
R2975 T3673 T3715 arg2Of Membranes,incubated
R2976 T3675 T3676 arg1Of blocked,with
R2977 T3675 T3713 arg1Of blocked,and
R2978 T3678 T3676 arg2Of %,with
R2979 T3678 T3677 arg1Of %,2.5
R2980 T3678 T3679 arg1Of %,blocking
R2981 T3678 T3690 arg1Of %,(
R2982 T3680 T3679 arg2Of reagent,blocking
R2983 T3680 T3681 arg1Of reagent,(
R2984 T3680 T3687 arg1Of reagent,in
R2985 T3683 T3681 arg2Of Mannheim,(
R2986 T3683 T3682 arg1Of Mannheim,Boehringer
R2987 T3683 T3684 arg1Of Mannheim,","
R2988 T3685 T3684 arg2Of Germany,","
R2989 T3686 T3681 arg3Of ),(
R2990 T3689 T3687 arg2Of buffer,in
R2991 T3689 T3688 arg1Of buffer,TBST
R2992 T3693 T3691 arg1Of Tris–HCL,4.44
R2993 T3693 T3692 arg1Of Tris–HCL,g/l
R2994 T3693 T3694 arg1Of Tris–HCL,","
R2995 T3694 T3698 arg1Of ",",","
R2996 T3697 T3694 arg2Of TrisOH,","
R2997 T3697 T3695 arg1Of TrisOH,2.65
R2998 T3697 T3696 arg1Of TrisOH,g/l
R2999 T3698 T3702 arg1Of ",",","
R3000 T3701 T3698 arg2Of NaCl,","
R3001 T3701 T3699 arg1Of NaCl,8.07
R3002 T3701 T3700 arg1Of NaCl,g/l
R3003 T3702 T3706 arg1Of ",",and
R3004 T3703 T3704 arg1Of 0.2,g/l
R3005 T3705 T3702 arg2Of KCl,","
R3006 T3705 T3703 arg1Of KCl,0.2
R3007 T3706 T3708 arg1Of and,µl/l
R3008 T3707 T3706 arg2Of 500,and
R3009 T3708 T3690 arg2Of µl/l,(
R3010 T3708 T3710 arg1Of µl/l,in
R3011 T3709 T3708 arg2Of Tween-20,µl/l
R3012 T3711 T3710 arg2Of H2O,in
R3013 T3712 T3690 arg3Of ),(
R3014 T3713 T3674 arg2Of and,were
R3015 T3713 T3739 arg1Of and,respectively
R3016 T3715 T3713 arg2Of incubated,and
R3017 T3715 T3714 arg1Of incubated,subsequently
R3018 T3715 T3716 arg1Of incubated,with
R3019 T3715 T3738 arg1Of incubated,","
R3020 T3718 T3716 arg2Of antibody,with
R3021 T3718 T3717 arg1Of antibody,primary
R3022 T3718 T3719 arg1Of antibody,as
R3023 T3723 T3721 arg1Of peroxidase-conjugated,and
R3024 T3723 T3722 arg1Of peroxidase-conjugated,horseradish
R3025 T3725 T3720 arg2Of antibody,indicated
R3026 T3725 T3723 arg1Of antibody,peroxidase-conjugated
R3027 T3725 T3724 arg1Of antibody,secondary
R3028 T3725 T3726 arg1Of antibody,","
R3029 T3726 T3719 arg2Of ",",as
R3030 T3726 T3731 arg1Of ",",(
R3031 T3727 T3728 arg1Of anti-mouse,or
R3032 T3729 T3728 arg2Of anti-goat,or
R3033 T3730 T3726 arg2Of IgG,","
R3034 T3730 T3727 arg1Of IgG,anti-mouse
R3035 T3730 T3729 arg1Of IgG,anti-goat
R3036 T3732 T3733 arg1Of DAKO,","
R3037 T3733 T3735 arg1Of ",",","
R3038 T3734 T3733 arg2Of Hamburg,","
R3039 T3735 T3731 arg2Of ",",(
R3040 T3736 T3735 arg2Of Germany,","
R3041 T3737 T3731 arg3Of ),(
R3042 T3741 T3740 arg1Of membranes,The
R3043 T3741 T3742 arg1Of membranes,were
R3044 T3741 T3744 arg2Of membranes,developed
R3045 T3744 T3742 arg2Of developed,were
R3046 T3744 T3743 arg1Of developed,then
R3047 T3744 T3745 arg1Of developed,with
R3048 T3749 T3745 arg2Of kit,with
R3049 T3749 T3746 arg1Of kit,an
R3050 T3749 T3747 arg1Of kit,ECL
R3051 T3749 T3748 arg1Of kit,detection
R3052 T3749 T3750 arg1Of kit,(
R3053 T3753 T3750 arg2Of Biotech,(
R3054 T3753 T3751 arg1Of Biotech,Amersham
R3055 T3753 T3752 arg1Of Biotech,Pharmacia
R3056 T3753 T3754 arg1Of Biotech,","
R3057 T3755 T3754 arg2Of Freiburg,","
R3058 T3755 T3756 arg1Of Freiburg,","
R3059 T3757 T3756 arg2Of Germany,","
R3060 T3758 T3750 arg3Of ),(
R3061 T3761 T3759 arg1Of antibodies,The
R3062 T3761 T3760 arg1Of antibodies,primary
R3063 T3761 T3762 arg1Of antibodies,were
R3064 T3764 T3763 arg1Of anti-IRF-4/ICSAT,goat
R3065 T3764 T3765 arg1Of anti-IRF-4/ICSAT,(
R3066 T3764 T3768 arg1Of anti-IRF-4/ICSAT,(
R3067 T3764 T3778 arg1Of anti-IRF-4/ICSAT,and
R3068 T3766 T3765 arg2Of M-17,(
R3069 T3767 T3765 arg3Of ),(
R3070 T3771 T3768 arg2Of Biotechnology,(
R3071 T3771 T3769 arg1Of Biotechnology,Santa
R3072 T3771 T3770 arg1Of Biotechnology,Cruz
R3073 T3771 T3772 arg1Of Biotechnology,","
R3074 T3774 T3772 arg2Of Cruz,","
R3075 T3774 T3773 arg1Of Cruz,Santa
R3076 T3774 T3775 arg1Of Cruz,","
R3077 T3776 T3775 arg2Of CA,","
R3078 T3777 T3768 arg3Of ),(
R3079 T3778 T3762 arg2Of and,were
R3080 T3778 T3784 arg1Of and,(
R3081 T3782 T3778 arg2Of AC-74,and
R3082 T3782 T3779 arg1Of AC-74,mouse
R3083 T3782 T3780 arg1Of AC-74,anti-β-actin
R3084 T3782 T3781 arg2Of AC-74,(
R3085 T3783 T3781 arg3Of ),(
R3086 T3785 T3784 arg2Of Sigma,(
R3087 T3786 T3784 arg3Of ),(