PubMed:3427591 JSONTXT 23 Projects

F.t.-i.r. spectra of oligo- and poly-nucleotides. Fourier-transform infrared (F.t.-i.r.) spectra of some synthetic mono-, oligo-, and poly-nucleotides and a natural DNA extracted from crab gonad have been recorded. Assignments of the observed frequencies are proposed by reference to our previous interpretation of laser-Raman and F.t.-i.r. spectra of constituents of nucleic acids. The spectra reflected structural differences between poly[d(A-T).d(A-T)] and crab gonad DNA (which is extremely rich in d(A-T) base-pairing). Such differences are mainly due to the effect of hydration on the conformational properties of natural DNA. Although the degree of complexity of the molecules investigated is higher than that of mononucleotides, most of the characteristic i.r. bands remain recognizable and assignable by comparison with the spectra of nucleic acid constituents previously studied.