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PMC:549712 / 7859-29674 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T2692 9-19 NN denotes Generation
T2693 20-22 IN denotes of
T2694 23-28 NN denotes Ptdsr
T2696 28-29 HYPH denotes -
T2695 29-38 JJ denotes deficient
T2697 39-43 NNS denotes mice
T2698 43-199 sentence denotes To investigate in vivo the functions of the phosphatidylserine receptor Ptdsr, we generated a null allele in the mouse by gene targeting (Figure 1a,1b,1c).
T2699 44-46 TO denotes To
T2700 47-58 VB denotes investigate
T2702 59-61 FW denotes in
T2703 62-66 FW denotes vivo
T2704 67-70 DT denotes the
T2705 71-80 NNS denotes functions
T2706 81-83 IN denotes of
T2707 84-87 DT denotes the
T2709 88-106 NN denotes phosphatidylserine
T2708 107-115 NN denotes receptor
T2710 116-121 NN denotes Ptdsr
T2711 121-123 , denotes ,
T2712 123-125 PRP denotes we
T2701 126-135 VBD denotes generated
T2713 136-137 DT denotes a
T2715 138-142 JJ denotes null
T2714 143-149 NN denotes allele
T2716 150-152 IN denotes in
T2717 153-156 DT denotes the
T2718 157-162 NN denotes mouse
T2719 163-165 IN denotes by
T2720 166-170 NN denotes gene
T2721 171-180 NN denotes targeting
T2722 181-182 -LRB- denotes (
T2724 182-188 NN denotes Figure
T2725 189-191 NN denotes 1a
T2726 191-192 , denotes ,
T2727 192-194 NN denotes 1b
T2728 194-195 , denotes ,
T2723 195-197 NN denotes 1c
T2729 197-198 -RRB- denotes )
T2730 198-199 . denotes .
T2731 199-415 sentence denotes In contrast to previously described Ptdsr-knockout mice [31,32], we used Bruce4 embryonic stem (ES) cells for gene targeting [33], thus generating a Ptdsr-null allele in a pure, isogenic C57BL/6J genetic background.
T2732 200-202 IN denotes In
T2734 203-211 NN denotes contrast
T2735 212-214 IN denotes to
T2736 215-225 RB denotes previously
T2737 226-235 VBN denotes described
T2739 236-241 NN denotes Ptdsr
T2741 241-242 HYPH denotes -
T2740 242-250 NN denotes knockout
T2738 251-255 NNS denotes mice
T2742 256-257 -LRB- denotes [
T2744 257-259 CD denotes 31
T2745 259-260 , denotes ,
T2743 260-262 CD denotes 32
T2746 262-263 -RRB- denotes ]
T2747 263-265 , denotes ,
T2748 265-267 PRP denotes we
T2733 268-272 VBD denotes used
T2749 273-279 NN denotes Bruce4
T2751 280-289 JJ denotes embryonic
T2752 290-294 NN denotes stem
T2753 295-296 -LRB- denotes (
T2754 296-298 NN denotes ES
T2755 298-299 -RRB- denotes )
T2750 300-305 NNS denotes cells
T2756 306-309 IN denotes for
T2757 310-314 NN denotes gene
T2758 315-324 NN denotes targeting
T2759 325-326 -LRB- denotes [
T2760 326-328 CD denotes 33
T2761 328-329 -RRB- denotes ]
T2762 329-331 , denotes ,
T2763 331-335 RB denotes thus
T2764 336-346 VBG denotes generating
T2765 347-348 DT denotes a
T2767 349-354 NN denotes Ptdsr
T2769 354-355 HYPH denotes -
T2768 355-359 JJ denotes null
T2766 360-366 NN denotes allele
T2770 367-369 IN denotes in
T2771 370-371 DT denotes a
T2773 372-376 JJ denotes pure
T2774 376-378 , denotes ,
T2775 378-386 JJ denotes isogenic
T2776 387-392 NN denotes C57BL
T2778 392-393 HYPH denotes /
T2777 393-395 NN denotes 6J
T2779 396-403 JJ denotes genetic
T2772 404-414 NN denotes background
T2780 414-415 . denotes .
T2781 415-517 sentence denotes The newly established knockout mouse line was named Ptdsrtm1Gbf (hereafter referred to as Ptdsr -/-).
T2782 416-419 DT denotes The
T2784 420-425 RB denotes newly
T2785 426-437 VBN denotes established
T2786 438-446 NN denotes knockout
T2787 447-452 NN denotes mouse
T2783 453-457 NN denotes line
T2789 458-461 VBD denotes was
T2788 462-467 VBN denotes named
T2790 468-479 NN denotes Ptdsrtm1Gbf
T2791 480-481 -LRB- denotes (
T2793 481-490 RB denotes hereafter
T2792 491-499 VBN denotes referred
T2794 500-502 IN denotes to
T2795 503-505 IN denotes as
T2796 506-511 NN denotes Ptdsr
T2797 512-513 SYM denotes -
T2798 513-514 HYPH denotes /
T2799 514-515 SYM denotes -
T2800 515-516 -RRB- denotes )
T2801 516-517 . denotes .
T2802 517-605 sentence denotes Heterozygous Ptdsr+/- mice were viable and fertile and showed no obvious abnormalities.
T2803 518-530 JJ denotes Heterozygous
T2805 531-536 NN denotes Ptdsr
T2806 536-537 SYM denotes +
T2807 537-538 HYPH denotes /
T2808 538-539 SYM denotes -
T2804 540-544 NNS denotes mice
T2809 545-549 VBD denotes were
T2810 550-556 JJ denotes viable
T2811 557-560 CC denotes and
T2812 561-568 JJ denotes fertile
T2813 569-572 CC denotes and
T2814 573-579 VBD denotes showed
T2815 580-582 DT denotes no
T2817 583-590 JJ denotes obvious
T2816 591-604 NNS denotes abnormalities
T2818 604-605 . denotes .
T2819 605-683 sentence denotes Ptdsr +/- mice were intercrossed to generate homozygous Ptdsr-deficient mice.
T2820 606-611 NN denotes Ptdsr
T2822 612-613 SYM denotes +
T2823 613-614 HYPH denotes /
T2824 614-615 SYM denotes -
T2821 616-620 NNS denotes mice
T2826 621-625 VBD denotes were
T2825 626-638 VBN denotes intercrossed
T2827 639-641 TO denotes to
T2828 642-650 VB denotes generate
T2829 651-661 JJ denotes homozygous
T2831 662-667 NN denotes Ptdsr
T2833 667-668 HYPH denotes -
T2832 668-677 JJ denotes deficient
T2830 678-682 NNS denotes mice
T2834 682-683 . denotes .
T2835 683-839 sentence denotes The absence of Ptdsr expression in Ptdsr -/- embryos was confirmed by RT-PCR (data not shown), and by northern and western blotting analyses (Figure 1d,e).
T2836 684-687 DT denotes The
T2837 688-695 NN denotes absence
T2839 696-698 IN denotes of
T2840 699-704 NN denotes Ptdsr
T2841 705-715 NN denotes expression
T2842 716-718 IN denotes in
T2843 719-724 NN denotes Ptdsr
T2845 725-726 SYM denotes -
T2846 726-727 HYPH denotes /
T2847 727-728 SYM denotes -
T2844 729-736 NNS denotes embryos
T2848 737-740 VBD denotes was
T2838 741-750 VBN denotes confirmed
T2849 751-753 IN denotes by
T2850 754-756 NN denotes RT
T2852 756-757 HYPH denotes -
T2851 757-760 NN denotes PCR
T2853 761-762 -LRB- denotes (
T2855 762-766 NNS denotes data
T2856 767-770 RB denotes not
T2854 771-776 VBN denotes shown
T2857 776-777 -RRB- denotes )
T2858 777-779 , denotes ,
T2859 779-782 CC denotes and
T2860 783-785 IN denotes by
T2861 786-794 NNP denotes northern
T2863 795-798 CC denotes and
T2864 799-806 NNP denotes western
T2862 807-815 NN denotes blotting
T2865 816-824 NNS denotes analyses
T2866 825-826 -LRB- denotes (
T2868 826-832 NN denotes Figure
T2869 833-835 NN denotes 1d
T2870 835-836 , denotes ,
T2867 836-837 NN denotes e
T2871 837-838 -RRB- denotes )
T2872 838-839 . denotes .
T2873 839-995 sentence denotes Interbreeding of heterozygous mice showed that the mutation was lethal, since homozygous mutants were not detected in over 100 analyzed litters at weaning.
T2874 840-853 NN denotes Interbreeding
T2876 854-856 IN denotes of
T2877 857-869 JJ denotes heterozygous
T2878 870-874 NNS denotes mice
T2875 875-881 VBD denotes showed
T2879 882-886 IN denotes that
T2881 887-890 DT denotes the
T2882 891-899 NN denotes mutation
T2880 900-903 VBD denotes was
T2883 904-910 JJ denotes lethal
T2884 910-912 , denotes ,
T2885 912-917 IN denotes since
T2887 918-928 JJ denotes homozygous
T2888 929-936 NNS denotes mutants
T2889 937-941 VBD denotes were
T2890 942-945 RB denotes not
T2886 946-954 VBN denotes detected
T2891 955-957 IN denotes in
T2892 958-962 IN denotes over
T2893 963-966 CD denotes 100
T2895 967-975 VBN denotes analyzed
T2894 976-983 NNS denotes litters
T2896 984-986 IN denotes at
T2897 987-994 NN denotes weaning
T2898 994-995 . denotes .
T2899 995-1154 sentence denotes To determine the stages of embryonic development affected by the Ptdsrtm1Gbf mutation, timed breedings were followed by PCR genotyping (Figure 1c) of embryos.
T2900 996-998 TO denotes To
T2901 999-1008 VB denotes determine
T2903 1009-1012 DT denotes the
T2904 1013-1019 NNS denotes stages
T2905 1020-1022 IN denotes of
T2906 1023-1032 JJ denotes embryonic
T2907 1033-1044 NN denotes development
T2908 1045-1053 VBN denotes affected
T2909 1054-1056 IN denotes by
T2910 1057-1060 DT denotes the
T2912 1061-1072 NN denotes Ptdsrtm1Gbf
T2911 1073-1081 NN denotes mutation
T2913 1081-1083 , denotes ,
T2914 1083-1088 VBN denotes timed
T2915 1089-1098 NNS denotes breedings
T2916 1099-1103 VBD denotes were
T2902 1104-1112 VBN denotes followed
T2917 1113-1115 IN denotes by
T2918 1116-1119 NN denotes PCR
T2919 1120-1130 NN denotes genotyping
T2920 1131-1132 -LRB- denotes (
T2922 1132-1138 NN denotes Figure
T2921 1139-1141 NN denotes 1c
T2923 1141-1142 -RRB- denotes )
T2924 1143-1145 IN denotes of
T2925 1146-1153 NNS denotes embryos
T2926 1153-1154 . denotes .
T2927 1154-1255 sentence denotes We recovered fewer than the expected number of homozygous embryos from intercrosses of Ptdsr+/-mice.
T2928 1155-1157 PRP denotes We
T2929 1158-1167 VBD denotes recovered
T2930 1168-1173 JJR denotes fewer
T2931 1174-1178 IN denotes than
T2932 1179-1182 DT denotes the
T2934 1183-1191 VBN denotes expected
T2933 1192-1198 NN denotes number
T2935 1199-1201 IN denotes of
T2936 1202-1212 JJ denotes homozygous
T2937 1213-1220 NNS denotes embryos
T2938 1221-1225 IN denotes from
T2939 1226-1238 NNS denotes intercrosses
T2940 1239-1241 IN denotes of
T2941 1242-1247 NN denotes Ptdsr
T2943 1247-1248 SYM denotes +
T2944 1248-1249 HYPH denotes /
T2945 1249-1250 SYM denotes -
T2942 1250-1254 NNS denotes mice
T2946 1254-1255 . denotes .
T2947 1255-1503 sentence denotes From a total of 1,031 embryos analyzed between gestational day (E) 9.5 and E18.5, 198 (19.2%) Ptdsr-deficient homozygous embryos were harvested, indicating that the introduced mutation is associated with a low rate of embryonic lethality in utero.
T2948 1256-1260 IN denotes From
T2950 1261-1262 DT denotes a
T2951 1263-1268 NN denotes total
T2952 1269-1271 IN denotes of
T2953 1272-1277 CD denotes 1,031
T2954 1278-1285 NNS denotes embryos
T2955 1286-1294 VBN denotes analyzed
T2956 1295-1302 IN denotes between
T2957 1303-1314 JJ denotes gestational
T2959 1315-1318 NN denotes day
T2960 1319-1320 -LRB- denotes (
T2958 1320-1321 NN denotes E
T2961 1321-1322 -RRB- denotes )
T2962 1323-1326 CD denotes 9.5
T2963 1327-1330 CC denotes and
T2964 1331-1336 NN denotes E18.5
T2965 1336-1338 , denotes ,
T2966 1338-1341 CD denotes 198
T2968 1342-1343 -LRB- denotes (
T2970 1343-1347 CD denotes 19.2
T2969 1347-1348 NN denotes %
T2971 1348-1349 -RRB- denotes )
T2972 1350-1355 NN denotes Ptdsr
T2974 1355-1356 HYPH denotes -
T2973 1356-1365 JJ denotes deficient
T2975 1366-1376 JJ denotes homozygous
T2967 1377-1384 NNS denotes embryos
T2976 1385-1389 VBD denotes were
T2949 1390-1399 VBN denotes harvested
T2977 1399-1401 , denotes ,
T2978 1401-1411 VBG denotes indicating
T2979 1412-1416 IN denotes that
T2981 1417-1420 DT denotes the
T2983 1421-1431 VBN denotes introduced
T2982 1432-1440 NN denotes mutation
T2984 1441-1443 VBZ denotes is
T2980 1444-1454 VBN denotes associated
T2985 1455-1459 IN denotes with
T2986 1460-1461 DT denotes a
T2988 1462-1465 JJ denotes low
T2987 1466-1470 NN denotes rate
T2989 1471-1473 IN denotes of
T2990 1474-1483 JJ denotes embryonic
T2991 1484-1493 NN denotes lethality
T2992 1494-1496 FW denotes in
T2993 1497-1502 FW denotes utero
T2994 1502-1503 . denotes .
T2995 1503-1574 sentence denotes From E9.5 to E12.5, Ptdsr -/- embryos were viable and of normal size.
T2996 1504-1508 IN denotes From
T2998 1509-1513 NN denotes E9.5
T2999 1514-1516 IN denotes to
T3000 1517-1522 NN denotes E12.5
T3001 1522-1524 , denotes ,
T3002 1524-1529 NN denotes Ptdsr
T3004 1530-1531 SYM denotes -
T3005 1531-1532 HYPH denotes /
T3006 1532-1533 SYM denotes -
T3003 1535-1542 NNS denotes embryos
T2997 1543-1547 VBD denotes were
T3007 1548-1554 JJ denotes viable
T3008 1555-1558 CC denotes and
T3009 1559-1561 IN denotes of
T3010 1562-1568 JJ denotes normal
T3011 1569-1573 NN denotes size
T3012 1573-1574 . denotes .
T3013 1574-1676 sentence denotes At E13.5 and thereafter, however, most Ptdsr -/-embryos showed morphological abnormalities (Table 1).
T3014 1575-1577 IN denotes At
T3016 1578-1583 NN denotes E13.5
T3017 1584-1587 CC denotes and
T3018 1588-1598 RB denotes thereafter
T3019 1598-1600 , denotes ,
T3020 1600-1607 RB denotes however
T3021 1607-1609 , denotes ,
T3022 1609-1613 JJS denotes most
T3024 1614-1619 NN denotes Ptdsr
T3025 1620-1621 SYM denotes -
T3026 1621-1622 HYPH denotes /
T3027 1622-1623 SYM denotes -
T3023 1623-1630 NNS denotes embryos
T3015 1631-1637 VBD denotes showed
T3028 1638-1651 JJ denotes morphological
T3029 1652-1665 NNS denotes abnormalities
T3030 1666-1667 -LRB- denotes (
T3031 1667-1672 NN denotes Table
T3032 1673-1674 CD denotes 1
T3033 1674-1675 -RRB- denotes )
T3034 1675-1676 . denotes .
T3035 1676-1827 sentence denotes All homozygous embryos harvested were growth-retarded from E13.5 onwards, had a pale appearance, and displayed multiple developmental dysmorphologies.
T3036 1677-1680 DT denotes All
T3038 1681-1691 JJ denotes homozygous
T3037 1692-1699 NNS denotes embryos
T3040 1700-1709 VBN denotes harvested
T3039 1710-1714 VBD denotes were
T3041 1715-1721 NN denotes growth
T3043 1721-1722 HYPH denotes -
T3042 1722-1730 VBN denotes retarded
T3044 1731-1735 IN denotes from
T3045 1736-1741 NN denotes E13.5
T3046 1742-1749 RB denotes onwards
T3047 1749-1751 , denotes ,
T3048 1751-1754 VBD denotes had
T3049 1755-1756 DT denotes a
T3051 1757-1761 JJ denotes pale
T3050 1762-1772 NN denotes appearance
T3052 1772-1774 , denotes ,
T3053 1774-1777 CC denotes and
T3054 1778-1787 VBD denotes displayed
T3055 1788-1796 JJ denotes multiple
T3057 1797-1810 JJ denotes developmental
T3056 1811-1826 NNS denotes dysmorphologies
T3058 1826-1827 . denotes .
T3059 1827-1960 sentence denotes These included various head and craniofacial malformations, such as exencephaly, cleft palate and abnormal head shape (Figure 1f,g).
T3060 1828-1833 DT denotes These
T3061 1834-1842 VBD denotes included
T3062 1843-1850 JJ denotes various
T3064 1851-1855 NN denotes head
T3065 1856-1859 CC denotes and
T3066 1860-1872 JJ denotes craniofacial
T3063 1873-1886 NNS denotes malformations
T3067 1886-1888 , denotes ,
T3068 1888-1892 JJ denotes such
T3069 1893-1895 IN denotes as
T3070 1896-1907 NN denotes exencephaly
T3071 1907-1909 , denotes ,
T3072 1909-1914 JJ denotes cleft
T3073 1915-1921 NN denotes palate
T3074 1922-1925 CC denotes and
T3075 1926-1934 JJ denotes abnormal
T3077 1935-1939 NN denotes head
T3076 1940-1945 NN denotes shape
T3078 1946-1947 -LRB- denotes (
T3080 1947-1953 NN denotes Figure
T3081 1954-1956 NN denotes 1f
T3082 1956-1957 , denotes ,
T3079 1957-1958 NN denotes g
T3083 1958-1959 -RRB- denotes )
T3084 1959-1960 . denotes .
T3085 1960-2061 sentence denotes Gross inspection revealed that eye development was severely affected in 14.1% of homozygous embryos.
T3086 1961-1966 JJ denotes Gross
T3087 1967-1977 NN denotes inspection
T3088 1978-1986 VBD denotes revealed
T3089 1987-1991 IN denotes that
T3091 1992-1995 NN denotes eye
T3092 1996-2007 NN denotes development
T3093 2008-2011 VBD denotes was
T3094 2012-2020 RB denotes severely
T3090 2021-2029 VBN denotes affected
T3095 2030-2032 IN denotes in
T3096 2033-2037 CD denotes 14.1
T3097 2037-2038 NN denotes %
T3098 2039-2041 IN denotes of
T3099 2042-2052 JJ denotes homozygous
T3100 2053-2060 NNS denotes embryos
T3101 2060-2061 . denotes .
T3102 2061-2220 sentence denotes The affected animals displayed a complete unilateral or bilateral absence of the eyes (Table 1) that was never detected in Ptdsr +/+ or Ptdsr +/- littermates.
T3103 2062-2065 DT denotes The
T3105 2066-2074 VBN denotes affected
T3104 2075-2082 NNS denotes animals
T3106 2083-2092 VBD denotes displayed
T3107 2093-2094 DT denotes a
T3109 2095-2103 JJ denotes complete
T3110 2104-2114 JJ denotes unilateral
T3111 2115-2117 CC denotes or
T3112 2118-2127 JJ denotes bilateral
T3108 2128-2135 NN denotes absence
T3113 2136-2138 IN denotes of
T3114 2139-2142 DT denotes the
T3115 2143-2147 NNS denotes eyes
T3116 2148-2149 -LRB- denotes (
T3117 2149-2154 NN denotes Table
T3118 2155-2156 CD denotes 1
T3119 2156-2157 -RRB- denotes )
T3120 2158-2162 WDT denotes that
T3122 2163-2166 VBD denotes was
T3123 2167-2172 RB denotes never
T3121 2173-2181 VBN denotes detected
T3124 2182-2184 IN denotes in
T3125 2185-2190 NN denotes Ptdsr
T3127 2191-2192 SYM denotes +
T3128 2192-2193 HYPH denotes /
T3129 2193-2194 SYM denotes +
T3130 2195-2197 CC denotes or
T3131 2198-2203 NN denotes Ptdsr
T3132 2204-2205 SYM denotes +
T3133 2205-2206 HYPH denotes /
T3134 2206-2207 SYM denotes -
T3126 2208-2219 NNS denotes littermates
T3135 2219-2220 . denotes .
T3136 2220-2324 sentence denotes Furthermore, homozygous embryos harvested between E12.5 and E15.5 had subcutaneous edema (Figure 1f,g).
T3137 2221-2232 RB denotes Furthermore
T3139 2232-2234 , denotes ,
T3140 2234-2244 JJ denotes homozygous
T3141 2245-2252 NNS denotes embryos
T3142 2253-2262 VBN denotes harvested
T3143 2263-2270 IN denotes between
T3144 2271-2276 NN denotes E12.5
T3145 2277-2280 CC denotes and
T3146 2281-2286 NN denotes E15.5
T3138 2287-2290 VBD denotes had
T3147 2291-2303 JJ denotes subcutaneous
T3148 2304-2309 NN denotes edema
T3149 2310-2311 -LRB- denotes (
T3151 2311-2317 NN denotes Figure
T3152 2318-2320 NN denotes 1f
T3153 2320-2321 , denotes ,
T3150 2321-2322 NN denotes g
T3154 2322-2323 -RRB- denotes )
T3155 2323-2324 . denotes .
T3156 2324-2452 sentence denotes Because we were able to recover Ptdsr -/- embryos until E18.5, we investigated whether Ptdsr-knockout mice could be born alive.
T3157 2325-2332 IN denotes Because
T3159 2333-2335 PRP denotes we
T3158 2336-2340 VBD denotes were
T3161 2341-2345 JJ denotes able
T3162 2346-2348 TO denotes to
T3163 2349-2356 VB denotes recover
T3164 2357-2362 NN denotes Ptdsr
T3166 2363-2364 SYM denotes -
T3167 2364-2365 HYPH denotes /
T3168 2365-2366 SYM denotes -
T3165 2367-2374 NNS denotes embryos
T3169 2375-2380 IN denotes until
T3170 2381-2386 NN denotes E18.5
T3171 2386-2388 , denotes ,
T3172 2388-2390 PRP denotes we
T3160 2391-2403 VBD denotes investigated
T3173 2404-2411 IN denotes whether
T3175 2412-2417 NN denotes Ptdsr
T3177 2417-2418 HYPH denotes -
T3176 2418-2426 NN denotes knockout
T3178 2427-2431 NNS denotes mice
T3179 2432-2437 MD denotes could
T3180 2438-2440 VB denotes be
T3174 2441-2445 VBN denotes born
T3181 2446-2451 JJ denotes alive
T3182 2451-2452 . denotes .
T3183 2452-2603 sentence denotes Careful observation of timed matings allowed us to recover Ptdsr -/- neonates, but homozygous pups died during delivery or within minutes after birth.
T3184 2453-2460 JJ denotes Careful
T3185 2461-2472 NN denotes observation
T3187 2473-2475 IN denotes of
T3188 2476-2481 VBN denotes timed
T3189 2482-2489 NNS denotes matings
T3186 2490-2497 VBD denotes allowed
T3190 2498-2500 PRP denotes us
T3192 2501-2503 TO denotes to
T3191 2504-2511 VB denotes recover
T3193 2512-2517 NN denotes Ptdsr
T3195 2518-2519 SYM denotes -
T3196 2519-2520 HYPH denotes /
T3197 2520-2521 SYM denotes -
T3194 2522-2530 NNS denotes neonates
T3198 2530-2532 , denotes ,
T3199 2532-2535 CC denotes but
T3200 2536-2546 JJ denotes homozygous
T3201 2547-2551 NNS denotes pups
T3202 2552-2556 VBD denotes died
T3203 2557-2563 IN denotes during
T3204 2564-2572 NN denotes delivery
T3205 2573-2575 CC denotes or
T3206 2576-2582 IN denotes within
T3207 2583-2590 NNS denotes minutes
T3208 2591-2596 IN denotes after
T3209 2597-2602 NN denotes birth
T3210 2602-2603 . denotes .
T3211 2603-2714 sentence denotes Ptdsr-deficient neonates were also growth-retarded, had a pale appearance and displayed various malformations.
T3212 2604-2609 NN denotes Ptdsr
T3214 2609-2610 HYPH denotes -
T3213 2610-2619 JJ denotes deficient
T3215 2620-2628 NNS denotes neonates
T3216 2629-2633 VBD denotes were
T3217 2634-2638 RB denotes also
T3218 2639-2645 NN denotes growth
T3220 2645-2646 HYPH denotes -
T3219 2646-2654 VBN denotes retarded
T3221 2654-2656 , denotes ,
T3222 2656-2659 VBD denotes had
T3223 2660-2661 DT denotes a
T3225 2662-2666 JJ denotes pale
T3224 2667-2677 NN denotes appearance
T3226 2678-2681 CC denotes and
T3227 2682-2691 VBD denotes displayed
T3228 2692-2699 JJ denotes various
T3229 2700-2713 NNS denotes malformations
T3230 2713-2714 . denotes .
T3231 2714-2812 sentence denotes These included cleft palate, abnormal head shape, absence of eyes and edematous skin (Figure 1h).
T3232 2715-2720 DT denotes These
T3233 2721-2729 VBD denotes included
T3234 2730-2735 JJ denotes cleft
T3235 2736-2742 NN denotes palate
T3236 2742-2744 , denotes ,
T3237 2744-2752 JJ denotes abnormal
T3239 2753-2757 NN denotes head
T3238 2758-2763 NN denotes shape
T3240 2763-2765 , denotes ,
T3241 2765-2772 NN denotes absence
T3242 2773-2775 IN denotes of
T3243 2776-2780 NNS denotes eyes
T3244 2781-2784 CC denotes and
T3245 2785-2794 JJ denotes edematous
T3246 2795-2799 NN denotes skin
T3247 2800-2801 -LRB- denotes (
T3249 2801-2807 NN denotes Figure
T3248 2808-2810 NN denotes 1h
T3250 2810-2811 -RRB- denotes )
T3251 2811-2812 . denotes .
T3252 2812-2930 sentence denotes Thus, deletion of the Ptdsr gene resulted in perinatal lethality with variable severity and penetrance of phenotypes.
T3253 2813-2817 RB denotes Thus
T3255 2817-2819 , denotes ,
T3256 2819-2827 NN denotes deletion
T3257 2828-2830 IN denotes of
T3258 2831-2834 DT denotes the
T3260 2835-2840 NN denotes Ptdsr
T3259 2841-2845 NN denotes gene
T3254 2846-2854 VBD denotes resulted
T3261 2855-2857 IN denotes in
T3262 2858-2867 JJ denotes perinatal
T3263 2868-2877 NN denotes lethality
T3264 2878-2882 IN denotes with
T3265 2883-2891 JJ denotes variable
T3266 2892-2900 NN denotes severity
T3267 2901-2904 CC denotes and
T3268 2905-2915 NN denotes penetrance
T3269 2916-2918 IN denotes of
T3270 2919-2929 NNS denotes phenotypes
T3271 2929-2930 . denotes .
T3608 2932-2942 NN denotes Expression
T3609 2943-2945 IN denotes of
T3610 2946-2951 NN denotes Ptdsr
T3611 2952-2958 IN denotes during
T3612 2959-2972 NN denotes embryogenesis
T3613 2973-2976 CC denotes and
T3614 2977-2979 IN denotes in
T3615 2980-2985 JJ denotes adult
T3616 2986-2993 NNS denotes tissues
T3617 2993-3091 sentence denotes The observed perinatal lethality indicates that Ptdsr plays an important role during development.
T3618 2994-2997 DT denotes The
T3620 2998-3006 VBN denotes observed
T3621 3007-3016 JJ denotes perinatal
T3619 3017-3026 NN denotes lethality
T3622 3027-3036 VBZ denotes indicates
T3623 3037-3041 IN denotes that
T3625 3042-3047 NN denotes Ptdsr
T3624 3048-3053 VBZ denotes plays
T3626 3054-3056 DT denotes an
T3628 3057-3066 JJ denotes important
T3627 3067-3071 NN denotes role
T3629 3072-3078 IN denotes during
T3630 3079-3090 NN denotes development
T3631 3090-3091 . denotes .
T3632 3091-3282 sentence denotes Analysis by RT-PCR (data not shown) showed that Ptdsr is expressed early in development, because we were able to detect Ptdsr transcripts in ES cells and embryos at all developmental stages.
T3633 3092-3100 NN denotes Analysis
T3635 3101-3103 IN denotes by
T3636 3104-3106 NN denotes RT
T3638 3106-3107 HYPH denotes -
T3637 3107-3110 NN denotes PCR
T3639 3111-3112 -LRB- denotes (
T3641 3112-3116 NNS denotes data
T3642 3117-3120 RB denotes not
T3640 3121-3126 VBN denotes shown
T3643 3126-3127 -RRB- denotes )
T3634 3128-3134 VBD denotes showed
T3644 3135-3139 IN denotes that
T3646 3140-3145 NN denotes Ptdsr
T3647 3146-3148 VBZ denotes is
T3645 3149-3158 VBN denotes expressed
T3648 3159-3164 RB denotes early
T3649 3165-3167 IN denotes in
T3650 3168-3179 NN denotes development
T3651 3179-3181 , denotes ,
T3652 3181-3188 IN denotes because
T3654 3189-3191 PRP denotes we
T3653 3192-3196 VBD denotes were
T3655 3197-3201 JJ denotes able
T3656 3202-3204 TO denotes to
T3657 3205-3211 VB denotes detect
T3658 3212-3217 NN denotes Ptdsr
T3659 3218-3229 NNS denotes transcripts
T3660 3230-3232 IN denotes in
T3661 3233-3235 NN denotes ES
T3662 3236-3241 NNS denotes cells
T3663 3242-3245 CC denotes and
T3664 3246-3253 NNS denotes embryos
T3665 3254-3256 IN denotes at
T3666 3257-3260 DT denotes all
T3668 3261-3274 JJ denotes developmental
T3667 3275-3281 NNS denotes stages
T3669 3281-3282 . denotes .
T3670 3282-3549 sentence denotes To analyze in more detail the temporal and spatial expression patterns of Ptdsr, and to correlate expression patterns with observed pathological malformations, we made use of a Ptdsr-β-geo gene-trap reporter mouse line generated from a Ptdsr gene-trap ES cell clone.
T3671 3283-3285 TO denotes To
T3672 3286-3293 VB denotes analyze
T3674 3294-3296 IN denotes in
T3675 3297-3301 JJR denotes more
T3676 3302-3308 NN denotes detail
T3677 3309-3312 DT denotes the
T3679 3313-3321 JJ denotes temporal
T3680 3322-3325 CC denotes and
T3681 3326-3333 JJ denotes spatial
T3682 3334-3344 NN denotes expression
T3678 3345-3353 NNS denotes patterns
T3683 3354-3356 IN denotes of
T3684 3357-3362 NN denotes Ptdsr
T3685 3362-3364 , denotes ,
T3686 3364-3367 CC denotes and
T3687 3368-3370 TO denotes to
T3688 3371-3380 VB denotes correlate
T3689 3381-3391 NN denotes expression
T3690 3392-3400 NNS denotes patterns
T3691 3401-3405 IN denotes with
T3692 3406-3414 VBN denotes observed
T3694 3415-3427 JJ denotes pathological
T3693 3428-3441 NNS denotes malformations
T3695 3441-3443 , denotes ,
T3696 3443-3445 PRP denotes we
T3673 3446-3450 VBD denotes made
T3697 3451-3454 NN denotes use
T3698 3455-3457 IN denotes of
T3699 3458-3459 DT denotes a
T3701 3460-3465 NN denotes Ptdsr
T3703 3465-3466 HYPH denotes -
T3704 3466-3467 NN denotes β
T3705 3467-3468 HYPH denotes -
T3702 3468-3471 NN denotes geo
T3706 3472-3476 NN denotes gene
T3708 3476-3477 HYPH denotes -
T3707 3477-3481 NN denotes trap
T3709 3482-3490 NN denotes reporter
T3710 3491-3496 NN denotes mouse
T3700 3497-3501 NN denotes line
T3711 3502-3511 VBN denotes generated
T3712 3512-3516 IN denotes from
T3713 3517-3518 DT denotes a
T3715 3519-3524 NN denotes Ptdsr
T3716 3525-3529 NN denotes gene
T3718 3529-3530 HYPH denotes -
T3717 3530-3534 NN denotes trap
T3719 3535-3537 NN denotes ES
T3720 3538-3542 NN denotes cell
T3714 3543-3548 NN denotes clone
T3721 3548-3549 . denotes .
T3722 3549-3637 sentence denotes This line has an insertion of β-galactosidase in the 3' region of the gene (Figure 2a).
T3723 3550-3554 DT denotes This
T3724 3555-3559 NN denotes line
T3725 3560-3563 VBZ denotes has
T3726 3564-3566 DT denotes an
T3727 3567-3576 NN denotes insertion
T3728 3577-3579 IN denotes of
T3729 3580-3581 NN denotes β
T3731 3581-3582 HYPH denotes -
T3730 3582-3595 NN denotes galactosidase
T3732 3596-3598 IN denotes in
T3733 3599-3602 DT denotes the
T3735 3603-3604 CD denotes 3
T3736 3604-3605 SYM denotes '
T3734 3606-3612 NN denotes region
T3737 3613-3615 IN denotes of
T3738 3616-3619 DT denotes the
T3739 3620-3624 NN denotes gene
T3740 3625-3626 -LRB- denotes (
T3742 3626-3632 NN denotes Figure
T3741 3633-3635 NN denotes 2a
T3743 3635-3636 -RRB- denotes )
T3744 3636-3637 . denotes .
T3745 3637-3741 sentence denotes We first examined Ptdsr expression by X-Gal staining in heterozygous embryos staged from E9.5 to E12.5.
T3746 3638-3640 PRP denotes We
T3748 3641-3646 RB denotes first
T3747 3647-3655 VBD denotes examined
T3749 3656-3661 NN denotes Ptdsr
T3750 3662-3672 NN denotes expression
T3751 3673-3675 IN denotes by
T3752 3676-3677 NN denotes X
T3754 3677-3678 HYPH denotes -
T3753 3678-3681 NN denotes Gal
T3755 3682-3690 NN denotes staining
T3756 3691-3693 IN denotes in
T3757 3694-3706 JJ denotes heterozygous
T3758 3707-3714 NNS denotes embryos
T3759 3715-3721 VBN denotes staged
T3760 3722-3726 IN denotes from
T3761 3727-3731 NN denotes E9.5
T3762 3732-3734 IN denotes to
T3763 3735-3740 NN denotes E12.5
T3764 3740-3741 . denotes .
T3765 3741-3908 sentence denotes These developmental stages were chosen so as to investigate Ptdsr expression in affected organs prior to the onset of pathological malformations in Ptdsr -/- embryos.
T3766 3742-3747 DT denotes These
T3768 3748-3761 JJ denotes developmental
T3767 3762-3768 NNS denotes stages
T3770 3769-3773 VBD denotes were
T3769 3774-3780 VBN denotes chosen
T3771 3781-3783 RB denotes so
T3773 3784-3786 IN denotes as
T3774 3787-3789 TO denotes to
T3772 3790-3801 VB denotes investigate
T3775 3802-3807 NN denotes Ptdsr
T3776 3808-3818 NN denotes expression
T3777 3819-3821 IN denotes in
T3778 3822-3830 VBN denotes affected
T3779 3831-3837 NNS denotes organs
T3780 3838-3843 JJ denotes prior
T3781 3844-3846 IN denotes to
T3782 3847-3850 DT denotes the
T3783 3851-3856 NN denotes onset
T3784 3857-3859 IN denotes of
T3785 3860-3872 JJ denotes pathological
T3786 3873-3886 NNS denotes malformations
T3787 3887-3889 IN denotes in
T3788 3890-3895 NN denotes Ptdsr
T3790 3896-3897 SYM denotes -
T3791 3897-3898 HYPH denotes /
T3792 3898-3899 SYM denotes -
T3789 3900-3907 NNS denotes embryos
T3793 3907-3908 . denotes .
T3794 3908-4027 sentence denotes At E9.5 we found Ptdsr expression in the developing neural tube, somites, heart, gut and branchial arches (Figure 2b).
T3795 3909-3911 IN denotes At
T3797 3912-3916 NN denotes E9.5
T3798 3917-3919 PRP denotes we
T3796 3920-3925 VBD denotes found
T3799 3926-3931 NN denotes Ptdsr
T3800 3932-3942 NN denotes expression
T3801 3943-3945 IN denotes in
T3802 3946-3949 DT denotes the
T3804 3950-3960 VBG denotes developing
T3805 3961-3967 JJ denotes neural
T3803 3968-3972 NN denotes tube
T3806 3972-3974 , denotes ,
T3807 3974-3981 NNS denotes somites
T3808 3981-3983 , denotes ,
T3809 3983-3988 NN denotes heart
T3810 3988-3990 , denotes ,
T3811 3990-3993 NN denotes gut
T3812 3994-3997 CC denotes and
T3813 3998-4007 JJ denotes branchial
T3814 4008-4014 NNS denotes arches
T3815 4015-4016 -LRB- denotes (
T3817 4016-4022 NN denotes Figure
T3816 4023-4025 NN denotes 2b
T3818 4025-4026 -RRB- denotes )
T3819 4026-4027 . denotes .
T3820 4027-4174 sentence denotes At E10.5, Ptdsr expression remained high in the developing nervous system, with most intense staining in the forebrain, hindbrain and neural tube.
T3821 4028-4030 IN denotes At
T3823 4031-4036 NN denotes E10.5
T3824 4036-4038 , denotes ,
T3825 4038-4043 NN denotes Ptdsr
T3826 4044-4054 NN denotes expression
T3822 4055-4063 VBD denotes remained
T3827 4064-4068 JJ denotes high
T3828 4069-4071 IN denotes in
T3829 4072-4075 DT denotes the
T3831 4076-4086 VBG denotes developing
T3832 4087-4094 JJ denotes nervous
T3830 4095-4101 NN denotes system
T3833 4101-4103 , denotes ,
T3834 4103-4107 IN denotes with
T3835 4108-4112 RBS denotes most
T3837 4113-4120 JJ denotes intense
T3836 4121-4129 NN denotes staining
T3838 4130-4132 IN denotes in
T3839 4133-4136 DT denotes the
T3840 4137-4146 NN denotes forebrain
T3841 4146-4148 , denotes ,
T3842 4148-4157 NN denotes hindbrain
T3843 4158-4161 CC denotes and
T3844 4162-4168 JJ denotes neural
T3845 4169-4173 NN denotes tube
T3846 4173-4174 . denotes .
T3847 4174-4311 sentence denotes At this stage of embryogenesis, high levels of Ptdsr expression could also be detected in the developing limb buds and eyes (Figure 2b).
T3848 4175-4177 IN denotes At
T3850 4178-4182 DT denotes this
T3851 4183-4188 NN denotes stage
T3852 4189-4191 IN denotes of
T3853 4192-4205 NN denotes embryogenesis
T3854 4205-4207 , denotes ,
T3855 4207-4211 JJ denotes high
T3856 4212-4218 NNS denotes levels
T3857 4219-4221 IN denotes of
T3858 4222-4227 NN denotes Ptdsr
T3859 4228-4238 NN denotes expression
T3860 4239-4244 MD denotes could
T3861 4245-4249 RB denotes also
T3862 4250-4252 VB denotes be
T3849 4253-4261 VBN denotes detected
T3863 4262-4264 IN denotes in
T3864 4265-4268 DT denotes the
T3866 4269-4279 VBG denotes developing
T3867 4280-4284 NN denotes limb
T3865 4285-4289 NNS denotes buds
T3868 4290-4293 CC denotes and
T3869 4294-4298 NNS denotes eyes
T3870 4299-4300 -LRB- denotes (
T3872 4300-4306 NN denotes Figure
T3871 4307-4309 NN denotes 2b
T3873 4309-4310 -RRB- denotes )
T3874 4310-4311 . denotes .
T3875 4311-4486 sentence denotes Ptdsr expression was altered at E12.5, with most intensive β-galactosidase staining in the eyes, developing condensations of the limb buds, neural tube and brain (Figure 2b).
T3876 4312-4317 NN denotes Ptdsr
T3877 4318-4328 NN denotes expression
T3879 4329-4332 VBD denotes was
T3878 4333-4340 VBN denotes altered
T3880 4341-4343 IN denotes at
T3881 4344-4349 NN denotes E12.5
T3882 4349-4351 , denotes ,
T3883 4351-4355 IN denotes with
T3884 4356-4360 JJS denotes most
T3886 4361-4370 JJ denotes intensive
T3887 4371-4372 NN denotes β
T3889 4372-4373 HYPH denotes -
T3888 4373-4386 NN denotes galactosidase
T3885 4387-4395 NN denotes staining
T3890 4396-4398 IN denotes in
T3891 4399-4402 DT denotes the
T3892 4403-4407 NNS denotes eyes
T3893 4407-4409 , denotes ,
T3894 4409-4419 VBG denotes developing
T3895 4420-4433 NNS denotes condensations
T3896 4434-4436 IN denotes of
T3897 4437-4440 DT denotes the
T3899 4441-4445 NN denotes limb
T3898 4446-4450 NNS denotes buds
T3900 4450-4452 , denotes ,
T3901 4452-4458 JJ denotes neural
T3902 4459-4463 NN denotes tube
T3903 4464-4467 CC denotes and
T3904 4468-4473 NN denotes brain
T3905 4474-4475 -LRB- denotes (
T3907 4475-4481 NN denotes Figure
T3906 4482-4484 NN denotes 2b
T3908 4484-4485 -RRB- denotes )
T3909 4485-4486 . denotes .
T3910 4486-4747 sentence denotes Transverse sections of X-Gal-stained embryos at E12.5 showed an asymmetric expression pattern in the neural tube with intense staining of the central mantle layer but no expression in the dorsal part of the neural tube (for example, the roof plate; Figure 2c).
T3911 4487-4497 JJ denotes Transverse
T3912 4498-4506 NNS denotes sections
T3914 4507-4509 IN denotes of
T3915 4510-4511 NN denotes X
T3917 4511-4512 HYPH denotes -
T3916 4512-4515 NN denotes Gal
T3919 4515-4516 HYPH denotes -
T3918 4516-4523 VBN denotes stained
T3920 4524-4531 NNS denotes embryos
T3921 4532-4534 IN denotes at
T3922 4535-4540 NN denotes E12.5
T3913 4541-4547 VBD denotes showed
T3923 4548-4550 DT denotes an
T3925 4551-4561 JJ denotes asymmetric
T3926 4562-4572 NN denotes expression
T3924 4573-4580 NN denotes pattern
T3927 4581-4583 IN denotes in
T3928 4584-4587 DT denotes the
T3930 4588-4594 JJ denotes neural
T3929 4595-4599 NN denotes tube
T3931 4600-4604 IN denotes with
T3932 4605-4612 JJ denotes intense
T3933 4613-4621 NN denotes staining
T3934 4622-4624 IN denotes of
T3935 4625-4628 DT denotes the
T3937 4629-4636 JJ denotes central
T3938 4637-4643 NN denotes mantle
T3936 4644-4649 NN denotes layer
T3939 4650-4653 CC denotes but
T3940 4654-4656 DT denotes no
T3941 4657-4667 NN denotes expression
T3942 4668-4670 IN denotes in
T3943 4671-4674 DT denotes the
T3945 4675-4681 JJ denotes dorsal
T3944 4682-4686 NN denotes part
T3946 4687-4689 IN denotes of
T3947 4690-4693 DT denotes the
T3949 4694-4700 JJ denotes neural
T3948 4701-4705 NN denotes tube
T3950 4706-4707 -LRB- denotes (
T3952 4707-4710 IN denotes for
T3953 4711-4718 NN denotes example
T3954 4718-4720 , denotes ,
T3955 4720-4723 DT denotes the
T3957 4724-4728 NN denotes roof
T3956 4729-4734 NN denotes plate
T3958 4734-4735 : denotes ;
T3959 4736-4742 NN denotes Figure
T3951 4743-4745 NN denotes 2c
T3960 4745-4746 -RRB- denotes )
T3961 4746-4747 . denotes .
T3962 4747-4941 sentence denotes Expression in dorsal root ganglia lateral to the neural tube and in the somites was observed; Ptdsr was expressed throughout the somite structure (myotome, dermatome and sclerotome; Figure 2d).
T3963 4748-4758 NN denotes Expression
T3965 4759-4761 IN denotes in
T3966 4762-4768 JJ denotes dorsal
T3968 4769-4773 NN denotes root
T3967 4774-4781 NNS denotes ganglia
T3969 4782-4789 JJ denotes lateral
T3970 4790-4792 IN denotes to
T3971 4793-4796 DT denotes the
T3973 4797-4803 JJ denotes neural
T3972 4804-4808 NN denotes tube
T3974 4809-4812 CC denotes and
T3975 4813-4815 IN denotes in
T3976 4816-4819 DT denotes the
T3977 4820-4827 NNS denotes somites
T3978 4828-4831 VBD denotes was
T3964 4832-4840 VBN denotes observed
T3980 4840-4841 : denotes ;
T3981 4842-4847 NN denotes Ptdsr
T3982 4848-4851 VBD denotes was
T3979 4852-4861 VBN denotes expressed
T3983 4862-4872 IN denotes throughout
T3984 4873-4876 DT denotes the
T3986 4877-4883 NN denotes somite
T3985 4884-4893 NN denotes structure
T3987 4894-4895 -LRB- denotes (
T3989 4895-4902 NN denotes myotome
T3990 4902-4904 , denotes ,
T3991 4904-4913 NN denotes dermatome
T3992 4914-4917 CC denotes and
T3993 4918-4928 NN denotes sclerotome
T3994 4928-4929 : denotes ;
T3995 4930-4936 NN denotes Figure
T3988 4937-4939 NN denotes 2d
T3996 4939-4940 -RRB- denotes )
T3997 4940-4941 . denotes .
T3998 4941-5111 sentence denotes Expression boundaries between somites were evident, with no expression in the segmental interzones, which correspond to the prospective intervertebral discs (Figure 2d).
T3999 4942-4952 NN denotes Expression
T4000 4953-4963 NNS denotes boundaries
T4002 4964-4971 IN denotes between
T4003 4972-4979 NNS denotes somites
T4001 4980-4984 VBD denotes were
T4004 4985-4992 JJ denotes evident
T4005 4992-4994 , denotes ,
T4006 4994-4998 IN denotes with
T4007 4999-5001 DT denotes no
T4008 5002-5012 NN denotes expression
T4009 5013-5015 IN denotes in
T4010 5016-5019 DT denotes the
T4012 5020-5029 JJ denotes segmental
T4011 5030-5040 NNS denotes interzones
T4013 5040-5042 , denotes ,
T4014 5042-5047 WDT denotes which
T4015 5048-5058 VBP denotes correspond
T4016 5059-5061 IN denotes to
T4017 5062-5065 DT denotes the
T4019 5066-5077 JJ denotes prospective
T4020 5078-5092 JJ denotes intervertebral
T4018 5093-5098 NNS denotes discs
T4021 5099-5100 -LRB- denotes (
T4023 5100-5106 NN denotes Figure
T4022 5107-5109 NN denotes 2d
T4024 5109-5110 -RRB- denotes )
T4025 5110-5111 . denotes .
T4026 5111-5282 sentence denotes Transverse sections of the developing eye at E12.5 revealed strong Ptdsr expression in the inner layer of the neural cup, which will later develop into the neural retina.
T4027 5112-5122 JJ denotes Transverse
T4028 5123-5131 NNS denotes sections
T4030 5132-5134 IN denotes of
T4031 5135-5138 DT denotes the
T4033 5139-5149 VBG denotes developing
T4032 5150-5153 NN denotes eye
T4034 5154-5156 IN denotes at
T4035 5157-5162 NN denotes E12.5
T4029 5163-5171 VBD denotes revealed
T4036 5172-5178 JJ denotes strong
T4038 5179-5184 NN denotes Ptdsr
T4037 5185-5195 NN denotes expression
T4039 5196-5198 IN denotes in
T4040 5199-5202 DT denotes the
T4042 5203-5208 JJ denotes inner
T4041 5209-5214 NN denotes layer
T4043 5215-5217 IN denotes of
T4044 5218-5221 DT denotes the
T4046 5222-5228 JJ denotes neural
T4045 5229-5232 NN denotes cup
T4047 5232-5234 , denotes ,
T4048 5234-5239 WDT denotes which
T4050 5240-5244 MD denotes will
T4051 5245-5250 RB denotes later
T4049 5251-5258 VB denotes develop
T4052 5259-5263 IN denotes into
T4053 5264-5267 DT denotes the
T4055 5268-5274 JJ denotes neural
T4054 5275-5281 NN denotes retina
T4056 5281-5282 . denotes .
T4057 5282-5393 sentence denotes Furthermore, Ptdsr expression was detected in the primary lens fiber cells of the developing lens (Figure 2e).
T4058 5283-5294 RB denotes Furthermore
T4060 5294-5296 , denotes ,
T4061 5296-5301 NN denotes Ptdsr
T4062 5302-5312 NN denotes expression
T4063 5313-5316 VBD denotes was
T4059 5317-5325 VBN denotes detected
T4064 5326-5328 IN denotes in
T4065 5329-5332 DT denotes the
T4067 5333-5340 JJ denotes primary
T4068 5341-5345 NN denotes lens
T4069 5346-5351 NN denotes fiber
T4066 5352-5357 NNS denotes cells
T4070 5358-5360 IN denotes of
T4071 5361-5364 DT denotes the
T4073 5365-5375 VBG denotes developing
T4072 5376-5380 NN denotes lens
T4074 5381-5382 -LRB- denotes (
T4076 5382-5388 NN denotes Figure
T4075 5389-5391 NN denotes 2e
T4077 5391-5392 -RRB- denotes )
T4078 5392-5393 . denotes .
T4079 5393-5652 sentence denotes We carefully investigated whether Ptdsr is expressed from E10.5 to E12.5 in the developing kidney and lungs, but no expression could be detected indicating that Ptdsr expression is required only at later stages in the development of these organs (see below).
T4080 5394-5396 PRP denotes We
T4082 5397-5406 RB denotes carefully
T4081 5407-5419 VBD denotes investigated
T4083 5420-5427 IN denotes whether
T4085 5428-5433 NN denotes Ptdsr
T4086 5434-5436 VBZ denotes is
T4084 5437-5446 VBN denotes expressed
T4087 5447-5451 IN denotes from
T4088 5452-5457 NN denotes E10.5
T4089 5458-5460 IN denotes to
T4090 5461-5466 NN denotes E12.5
T4091 5467-5469 IN denotes in
T4092 5470-5473 DT denotes the
T4094 5474-5484 VBG denotes developing
T4093 5485-5491 NN denotes kidney
T4095 5492-5495 CC denotes and
T4096 5496-5501 NNS denotes lungs
T4097 5501-5503 , denotes ,
T4098 5503-5506 CC denotes but
T4099 5507-5509 DT denotes no
T4100 5510-5520 NN denotes expression
T4102 5521-5526 MD denotes could
T4103 5527-5529 VB denotes be
T4101 5530-5538 VBN denotes detected
T4104 5539-5549 VBG denotes indicating
T4105 5550-5554 IN denotes that
T4107 5555-5560 NN denotes Ptdsr
T4108 5561-5571 NN denotes expression
T4109 5572-5574 VBZ denotes is
T4106 5575-5583 VBN denotes required
T4110 5584-5588 RB denotes only
T4111 5589-5591 IN denotes at
T4112 5592-5597 JJR denotes later
T4113 5598-5604 NNS denotes stages
T4114 5605-5607 IN denotes in
T4115 5608-5611 DT denotes the
T4116 5612-5623 NN denotes development
T4117 5624-5626 IN denotes of
T4118 5627-5632 DT denotes these
T4119 5633-5639 NNS denotes organs
T4120 5640-5641 -LRB- denotes (
T4121 5641-5644 VB denotes see
T4122 5645-5650 RB denotes below
T4123 5650-5651 -RRB- denotes )
T4124 5651-5652 . denotes .
T4125 5652-5805 sentence denotes Hybridization of a multiple-tissue northern blot revealed a single transcript of about 1.8 kb in almost every tissue analyzed in adult mice (Figure 2f).
T4126 5653-5666 NN denotes Hybridization
T4128 5667-5669 IN denotes of
T4129 5670-5671 DT denotes a
T4131 5672-5680 JJ denotes multiple
T4133 5680-5681 HYPH denotes -
T4132 5681-5687 NN denotes tissue
T4134 5688-5696 NNP denotes northern
T4130 5697-5701 NN denotes blot
T4127 5702-5710 VBD denotes revealed
T4135 5711-5712 DT denotes a
T4137 5713-5719 JJ denotes single
T4136 5720-5730 NN denotes transcript
T4138 5731-5733 IN denotes of
T4139 5734-5739 IN denotes about
T4140 5740-5743 CD denotes 1.8
T4141 5744-5746 NNS denotes kb
T4142 5747-5749 IN denotes in
T4143 5750-5756 RB denotes almost
T4145 5757-5762 DT denotes every
T4144 5763-5769 NN denotes tissue
T4146 5770-5778 VBN denotes analyzed
T4147 5779-5781 IN denotes in
T4148 5782-5787 JJ denotes adult
T4149 5788-5792 NNS denotes mice
T4150 5793-5794 -LRB- denotes (
T4152 5794-5800 NN denotes Figure
T4151 5801-5803 NN denotes 2f
T4153 5803-5804 -RRB- denotes )
T4154 5804-5805 . denotes .
T4155 5805-5986 sentence denotes The most prominent expression was observed in testis, thymus, kidney, liver and skin, with moderate to low expression in lung, small intestine, spleen, stomach and skeletal muscle.
T4156 5806-5809 DT denotes The
T4158 5810-5814 RBS denotes most
T4159 5815-5824 JJ denotes prominent
T4157 5825-5835 NN denotes expression
T4161 5836-5839 VBD denotes was
T4160 5840-5848 VBN denotes observed
T4162 5849-5851 IN denotes in
T4163 5852-5858 NN denotes testis
T4164 5858-5860 , denotes ,
T4165 5860-5866 NN denotes thymus
T4166 5866-5868 , denotes ,
T4167 5868-5874 NN denotes kidney
T4168 5874-5876 , denotes ,
T4169 5876-5881 NN denotes liver
T4170 5882-5885 CC denotes and
T4171 5886-5890 NN denotes skin
T4172 5890-5892 , denotes ,
T4173 5892-5896 IN denotes with
T4174 5897-5905 JJ denotes moderate
T4176 5906-5908 IN denotes to
T4177 5909-5912 JJ denotes low
T4175 5913-5923 NN denotes expression
T4178 5924-5926 IN denotes in
T4179 5927-5931 NN denotes lung
T4180 5931-5933 , denotes ,
T4181 5933-5938 JJ denotes small
T4182 5939-5948 NN denotes intestine
T4183 5948-5950 , denotes ,
T4184 5950-5956 NN denotes spleen
T4185 5956-5958 , denotes ,
T4186 5958-5965 NN denotes stomach
T4187 5966-5969 CC denotes and
T4188 5970-5978 JJ denotes skeletal
T4189 5979-5985 NN denotes muscle
T4190 5985-5986 . denotes .
T4191 5986-6101 sentence denotes Thus, Ptdsr is ubiquitously expressed throughout embryogenesis and in adult tissues, although at different levels.
T4192 5987-5991 RB denotes Thus
T4194 5991-5993 , denotes ,
T4195 5993-5998 NN denotes Ptdsr
T4196 5999-6001 VBZ denotes is
T4197 6002-6014 RB denotes ubiquitously
T4193 6015-6024 VBN denotes expressed
T4198 6025-6035 IN denotes throughout
T4199 6036-6049 NN denotes embryogenesis
T4200 6050-6053 CC denotes and
T4201 6054-6056 IN denotes in
T4202 6057-6062 JJ denotes adult
T4203 6063-6070 NNS denotes tissues
T4204 6070-6072 , denotes ,
T4205 6072-6080 IN denotes although
T4206 6081-6083 IN denotes at
T4207 6084-6093 JJ denotes different
T4208 6094-6100 NNS denotes levels
T4209 6100-6101 . denotes .
T4518 6103-6108 NN denotes Ptdsr
T4520 6109-6111 VBZ denotes is
T4519 6112-6120 VBN denotes required
T4521 6121-6124 IN denotes for
T4522 6125-6131 JJ denotes normal
T4524 6132-6138 NN denotes tissue
T4525 6139-6142 CC denotes and
T4526 6143-6148 NN denotes organ
T4523 6149-6164 NN denotes differentiation
T4527 6164-6221 sentence denotes We next examined the role of Ptdsr in organ development.
T4528 6165-6167 PRP denotes We
T4530 6168-6172 RB denotes next
T4529 6173-6181 VBD denotes examined
T4531 6182-6185 DT denotes the
T4532 6186-6190 NN denotes role
T4533 6191-6193 IN denotes of
T4534 6194-6199 NN denotes Ptdsr
T4535 6200-6202 IN denotes in
T4536 6203-6208 NN denotes organ
T4537 6209-6220 NN denotes development
T4538 6220-6221 . denotes .
T4539 6221-6380 sentence denotes Serial histological sections of Ptdsr -/- and control embryos were taken to perform a detailed morphological analysis of all organ systems during development.
T4540 6222-6228 JJ denotes Serial
T4542 6229-6241 JJ denotes histological
T4541 6242-6250 NNS denotes sections
T4544 6251-6253 IN denotes of
T4545 6254-6259 NN denotes Ptdsr
T4547 6260-6261 SYM denotes -
T4548 6261-6262 HYPH denotes /
T4549 6262-6263 SYM denotes -
T4550 6264-6267 CC denotes and
T4551 6268-6275 NN denotes control
T4546 6276-6283 NNS denotes embryos
T4552 6284-6288 VBD denotes were
T4543 6289-6294 VBN denotes taken
T4553 6295-6297 TO denotes to
T4554 6298-6305 VB denotes perform
T4555 6306-6307 DT denotes a
T4557 6308-6316 JJ denotes detailed
T4558 6317-6330 JJ denotes morphological
T4556 6331-6339 NN denotes analysis
T4559 6340-6342 IN denotes of
T4560 6343-6346 DT denotes all
T4562 6347-6352 NN denotes organ
T4561 6353-6360 NNS denotes systems
T4563 6361-6367 IN denotes during
T4564 6368-6379 NN denotes development
T4565 6379-6380 . denotes .
T4566 6380-6491 sentence denotes A significant delay in organ and tissue differentiation was observed at E16.5 in lungs, kidneys and intestine.
T4567 6381-6382 DT denotes A
T4569 6383-6394 JJ denotes significant
T4568 6395-6400 NN denotes delay
T4571 6401-6403 IN denotes in
T4572 6404-6409 NN denotes organ
T4574 6410-6413 CC denotes and
T4575 6414-6420 NN denotes tissue
T4573 6421-6436 NN denotes differentiation
T4576 6437-6440 VBD denotes was
T4570 6441-6449 VBN denotes observed
T4577 6450-6452 IN denotes at
T4578 6453-6458 NN denotes E16.5
T4579 6459-6461 IN denotes in
T4580 6462-6467 NNS denotes lungs
T4581 6467-6469 , denotes ,
T4582 6469-6476 NNS denotes kidneys
T4583 6477-6480 CC denotes and
T4584 6481-6490 NN denotes intestine
T4585 6490-6491 . denotes .
T4586 6491-6580 sentence denotes Lungs of control littermates were properly developed with expanding alveoli (Figure 3a).
T4587 6492-6497 NNS denotes Lungs
T4589 6498-6500 IN denotes of
T4590 6501-6508 NN denotes control
T4591 6509-6520 NNS denotes littermates
T4588 6521-6525 VBD denotes were
T4592 6526-6534 RB denotes properly
T4593 6535-6544 JJ denotes developed
T4594 6545-6549 IN denotes with
T4595 6550-6559 VBG denotes expanding
T4596 6560-6567 NNS denotes alveoli
T4597 6568-6569 -LRB- denotes (
T4599 6569-6575 NN denotes Figure
T4598 6576-6578 NN denotes 3a
T4600 6578-6579 -RRB- denotes )
T4601 6579-6580 . denotes .
T4602 6580-6742 sentence denotes Terminal bronchi and bronchioles were already well developed, and terminally differentiated epithelial cells with cilia on the luminal cell surface were present.
T4603 6581-6589 JJ denotes Terminal
T4604 6590-6597 NNS denotes bronchi
T4606 6598-6601 CC denotes and
T4607 6602-6613 NNS denotes bronchioles
T4605 6614-6618 VBD denotes were
T4608 6619-6626 RB denotes already
T4609 6627-6631 RB denotes well
T4610 6632-6641 JJ denotes developed
T4611 6641-6643 , denotes ,
T4612 6643-6646 CC denotes and
T4613 6647-6657 RB denotes terminally
T4614 6658-6672 VBN denotes differentiated
T4616 6673-6683 JJ denotes epithelial
T4615 6684-6689 NNS denotes cells
T4618 6690-6694 IN denotes with
T4619 6695-6700 NNS denotes cilia
T4620 6701-6703 IN denotes on
T4621 6704-6707 DT denotes the
T4623 6708-6715 JJ denotes luminal
T4624 6716-6720 NN denotes cell
T4622 6721-6728 NN denotes surface
T4617 6729-6733 VBD denotes were
T4625 6734-6741 JJ denotes present
T4626 6741-6742 . denotes .
T4627 6742-6886 sentence denotes In contrast, almost no alveoli or bronchioles were present in Ptdsr -/- lungs, indicating a delay or arrest in lung sacculation and expansion.
T4628 6743-6745 IN denotes In
T4630 6746-6754 NN denotes contrast
T4631 6754-6756 , denotes ,
T4632 6756-6762 RB denotes almost
T4634 6763-6765 DT denotes no
T4633 6766-6773 NNS denotes alveoli
T4635 6774-6776 CC denotes or
T4636 6777-6788 NNS denotes bronchioles
T4629 6789-6793 VBD denotes were
T4637 6794-6801 JJ denotes present
T4638 6802-6804 IN denotes in
T4639 6805-6810 NN denotes Ptdsr
T4641 6811-6812 SYM denotes -
T4642 6812-6813 HYPH denotes /
T4643 6813-6814 SYM denotes -
T4640 6816-6821 NNS denotes lungs
T4644 6821-6823 , denotes ,
T4645 6823-6833 VBG denotes indicating
T4646 6834-6835 DT denotes a
T4647 6836-6841 NN denotes delay
T4648 6842-6844 CC denotes or
T4649 6845-6851 NN denotes arrest
T4650 6852-6854 IN denotes in
T4651 6855-6859 NN denotes lung
T4652 6860-6871 NN denotes sacculation
T4653 6872-6875 CC denotes and
T4654 6876-6885 NN denotes expansion
T4655 6885-6886 . denotes .
T4656 6886-6977 sentence denotes Instead, we observed an abundance of mesenchyme that appeared highly immature (Figure 3g).
T4657 6887-6894 RB denotes Instead
T4659 6894-6896 , denotes ,
T4660 6896-6898 PRP denotes we
T4658 6899-6907 VBD denotes observed
T4661 6908-6910 DT denotes an
T4662 6911-6920 NN denotes abundance
T4663 6921-6923 IN denotes of
T4664 6924-6934 NN denotes mesenchyme
T4665 6935-6939 WDT denotes that
T4666 6940-6948 VBD denotes appeared
T4667 6949-6955 RB denotes highly
T4668 6956-6964 JJ denotes immature
T4669 6965-6966 -LRB- denotes (
T4671 6966-6972 NN denotes Figure
T4670 6973-6975 NN denotes 3g
T4672 6975-6976 -RRB- denotes )
T4673 6976-6977 . denotes .
T4674 6977-7078 sentence denotes A similar delay in tissue differentiation of Ptdsr -/- embryos was found in the kidneys (Figure 3h).
T4675 6978-6979 DT denotes A
T4677 6980-6987 JJ denotes similar
T4676 6988-6993 NN denotes delay
T4679 6994-6996 IN denotes in
T4680 6997-7003 NN denotes tissue
T4681 7004-7019 NN denotes differentiation
T4682 7020-7022 IN denotes of
T4683 7023-7028 NN denotes Ptdsr
T4685 7029-7030 SYM denotes -
T4686 7030-7031 HYPH denotes /
T4687 7031-7032 SYM denotes -
T4684 7033-7040 NNS denotes embryos
T4688 7041-7044 VBD denotes was
T4678 7045-7050 VBN denotes found
T4689 7051-7053 IN denotes in
T4690 7054-7057 DT denotes the
T4691 7058-7065 NNS denotes kidneys
T4692 7066-7067 -LRB- denotes (
T4694 7067-7073 NN denotes Figure
T4693 7074-7076 NN denotes 3h
T4695 7076-7077 -RRB- denotes )
T4696 7077-7078 . denotes .
T4697 7078-7278 sentence denotes Kidneys from Ptdsr+/+ embryos were well developed at E16.5, showing terminally differentiated glomeruli with Bowman's capsule and collecting tubules lined with cuboidal epithelial cells (Figure 3b).
T4698 7079-7086 NNS denotes Kidneys
T4700 7087-7091 IN denotes from
T4701 7092-7097 NN denotes Ptdsr
T4703 7097-7098 SYM denotes +
T4704 7098-7099 HYPH denotes /
T4705 7099-7100 SYM denotes +
T4702 7102-7109 NNS denotes embryos
T4699 7110-7114 VBD denotes were
T4706 7115-7119 RB denotes well
T4707 7120-7129 JJ denotes developed
T4708 7130-7132 IN denotes at
T4709 7133-7138 NN denotes E16.5
T4710 7138-7140 , denotes ,
T4711 7140-7147 VBG denotes showing
T4712 7148-7158 RB denotes terminally
T4713 7159-7173 VBN denotes differentiated
T4714 7174-7183 NNS denotes glomeruli
T4715 7184-7188 IN denotes with
T4716 7189-7195 NNP denotes Bowman
T4718 7195-7197 POS denotes 's
T4717 7198-7205 NN denotes capsule
T4719 7206-7209 CC denotes and
T4720 7210-7220 VBG denotes collecting
T4721 7221-7228 NNS denotes tubules
T4722 7229-7234 VBN denotes lined
T4723 7235-7239 IN denotes with
T4724 7240-7248 JJ denotes cuboidal
T4726 7249-7259 JJ denotes epithelial
T4725 7260-7265 NNS denotes cells
T4727 7266-7267 -LRB- denotes (
T4729 7267-7273 NN denotes Figure
T4728 7274-7276 NN denotes 3b
T4730 7276-7277 -RRB- denotes )
T4731 7277-7278 . denotes .
T4732 7278-7403 sentence denotes In contrast, Ptdsr-deficient kidneys had only primitive glomeruli at E16.5, and collecting tubules were less well-developed.
T4733 7279-7281 IN denotes In
T4735 7282-7290 NN denotes contrast
T4736 7290-7292 , denotes ,
T4737 7292-7297 NN denotes Ptdsr
T4739 7297-7298 SYM denotes -
T4738 7298-7307 JJ denotes deficient
T4740 7308-7315 NNS denotes kidneys
T4734 7316-7319 VBD denotes had
T4741 7320-7324 RB denotes only
T4743 7325-7334 JJ denotes primitive
T4742 7335-7344 NNS denotes glomeruli
T4744 7345-7347 IN denotes at
T4745 7348-7353 NN denotes E16.5
T4746 7353-7355 , denotes ,
T4747 7355-7358 CC denotes and
T4748 7359-7369 VBG denotes collecting
T4749 7370-7377 NNS denotes tubules
T4750 7378-7382 VBD denotes were
T4751 7383-7387 RBR denotes less
T4753 7388-7392 RB denotes well
T4754 7392-7393 HYPH denotes -
T4752 7393-7402 JJ denotes developed
T4755 7402-7403 . denotes .
T4756 7403-7504 sentence denotes Instead, a large amount of undifferentiated mesenchyme was present in Ptdsr -/- kidneys (Figure 3h).
T4757 7404-7411 RB denotes Instead
T4759 7411-7413 , denotes ,
T4760 7413-7414 DT denotes a
T4762 7415-7420 JJ denotes large
T4761 7421-7427 NN denotes amount
T4763 7428-7430 IN denotes of
T4764 7431-7447 JJ denotes undifferentiated
T4765 7448-7458 NN denotes mesenchyme
T4758 7459-7462 VBD denotes was
T4766 7463-7470 JJ denotes present
T4767 7471-7473 IN denotes in
T4768 7474-7479 NN denotes Ptdsr
T4770 7480-7481 SYM denotes -
T4771 7481-7482 HYPH denotes /
T4772 7482-7483 SYM denotes -
T4769 7484-7491 NNS denotes kidneys
T4773 7492-7493 -LRB- denotes (
T4775 7493-7499 NN denotes Figure
T4774 7500-7502 NN denotes 3h
T4776 7502-7503 -RRB- denotes )
T4777 7503-7504 . denotes .
T4778 7504-7600 sentence denotes A delay in tissue differentiation was also found in the intestine at this stage of development.
T4779 7505-7506 DT denotes A
T4780 7507-7512 NN denotes delay
T4782 7513-7515 IN denotes in
T4783 7516-7522 NN denotes tissue
T4784 7523-7538 NN denotes differentiation
T4785 7539-7542 VBD denotes was
T4786 7543-7547 RB denotes also
T4781 7548-7553 VBN denotes found
T4787 7554-7556 IN denotes in
T4788 7557-7560 DT denotes the
T4789 7561-7570 NN denotes intestine
T4790 7571-7573 IN denotes at
T4791 7574-7578 DT denotes this
T4792 7579-7584 NN denotes stage
T4793 7585-7587 IN denotes of
T4794 7588-7599 NN denotes development
T4795 7599-7600 . denotes .
T4796 7600-7710 sentence denotes Ptdsr -/- embryos displayed improperly developed villi and an underdeveloped or absent submucosa (Figure 3i).
T4797 7601-7606 NN denotes Ptdsr
T4799 7607-7608 SYM denotes -
T4800 7608-7609 HYPH denotes /
T4801 7609-7610 SYM denotes -
T4798 7611-7618 NNS denotes embryos
T4802 7619-7628 VBD denotes displayed
T4803 7629-7639 RB denotes improperly
T4804 7640-7649 VBN denotes developed
T4805 7650-7655 NNS denotes villi
T4806 7656-7659 CC denotes and
T4807 7660-7662 DT denotes an
T4809 7663-7677 JJ denotes underdeveloped
T4810 7678-7680 CC denotes or
T4811 7681-7687 JJ denotes absent
T4808 7688-7697 NN denotes submucosa
T4812 7698-7699 -LRB- denotes (
T4814 7699-7705 NN denotes Figure
T4813 7706-7708 NN denotes 3i
T4815 7708-7709 -RRB- denotes )
T4816 7709-7710 . denotes .
T4817 7710-7892 sentence denotes In wild-type embryos (Figure 3c), intestinal cellular differentiation was already highly organized, with intramural ganglion cells between the external and internal muscular layers.
T4818 7711-7713 IN denotes In
T4820 7714-7718 JJ denotes wild
T4822 7718-7719 HYPH denotes -
T4821 7719-7723 NN denotes type
T4823 7724-7731 NNS denotes embryos
T4824 7732-7733 -LRB- denotes (
T4826 7733-7739 NN denotes Figure
T4825 7740-7742 NN denotes 3c
T4827 7742-7743 -RRB- denotes )
T4828 7743-7745 , denotes ,
T4829 7745-7755 JJ denotes intestinal
T4831 7756-7764 JJ denotes cellular
T4830 7765-7780 NN denotes differentiation
T4819 7781-7784 VBD denotes was
T4832 7785-7792 RB denotes already
T4833 7793-7799 RB denotes highly
T4834 7800-7809 JJ denotes organized
T4835 7809-7811 , denotes ,
T4836 7811-7815 IN denotes with
T4837 7816-7826 JJ denotes intramural
T4839 7827-7835 NN denotes ganglion
T4838 7836-7841 NNS denotes cells
T4840 7842-7849 IN denotes between
T4841 7850-7853 DT denotes the
T4843 7854-7862 JJ denotes external
T4844 7863-7866 CC denotes and
T4845 7867-7875 JJ denotes internal
T4846 7876-7884 JJ denotes muscular
T4842 7885-7891 NNS denotes layers
T4847 7891-7892 . denotes .
T4848 7892-7986 sentence denotes Such neuronal cells were absent from the intestine of Ptdsr -/- embryos (Figure 3i), however.
T4849 7893-7897 JJ denotes Such
T4851 7898-7906 JJ denotes neuronal
T4850 7907-7912 NNS denotes cells
T4852 7913-7917 VBD denotes were
T4853 7918-7924 JJ denotes absent
T4854 7925-7929 IN denotes from
T4855 7930-7933 DT denotes the
T4856 7934-7943 NN denotes intestine
T4857 7944-7946 IN denotes of
T4858 7947-7952 NN denotes Ptdsr
T4860 7953-7954 SYM denotes -
T4861 7954-7955 HYPH denotes /
T4862 7955-7956 SYM denotes -
T4859 7957-7964 NNS denotes embryos
T4863 7965-7966 -LRB- denotes (
T4865 7966-7972 NN denotes Figure
T4864 7973-7975 NN denotes 3i
T4866 7975-7976 -RRB- denotes )
T4867 7976-7978 , denotes ,
T4868 7978-7985 RB denotes however
T4869 7985-7986 . denotes .
T4870 7986-8194 sentence denotes Some Ptdsr -/- mice (4.5 %) also displayed extensive brain malformations that resulted in externally visible head abnormalities, with occasional ectopic tissue outside the skull or exencephaly (Figure 1f,h).
T4871 7987-7991 DT denotes Some
T4873 7992-7997 NN denotes Ptdsr
T4874 7998-7999 SYM denotes -
T4875 7999-8000 HYPH denotes /
T4876 8000-8001 SYM denotes -
T4872 8002-8006 NNS denotes mice
T4878 8007-8008 -LRB- denotes (
T4880 8008-8011 CD denotes 4.5
T4879 8012-8013 NN denotes %
T4881 8013-8014 -RRB- denotes )
T4882 8015-8019 RB denotes also
T4877 8020-8029 VBD denotes displayed
T4883 8030-8039 JJ denotes extensive
T4885 8040-8045 NN denotes brain
T4884 8046-8059 NNS denotes malformations
T4886 8060-8064 WDT denotes that
T4887 8065-8073 VBD denotes resulted
T4888 8074-8076 IN denotes in
T4889 8077-8087 RB denotes externally
T4890 8088-8095 JJ denotes visible
T4892 8096-8100 NN denotes head
T4891 8101-8114 NNS denotes abnormalities
T4893 8114-8116 , denotes ,
T4894 8116-8120 IN denotes with
T4895 8121-8131 JJ denotes occasional
T4897 8132-8139 JJ denotes ectopic
T4896 8140-8146 NN denotes tissue
T4898 8147-8154 IN denotes outside
T4899 8155-8158 DT denotes the
T4900 8159-8164 NN denotes skull
T4901 8165-8167 CC denotes or
T4902 8168-8179 NN denotes exencephaly
T4903 8180-8181 -LRB- denotes (
T4905 8181-8187 NN denotes Figure
T4906 8188-8190 NN denotes 1f
T4907 8190-8191 , denotes ,
T4904 8191-8192 NN denotes h
T4908 8192-8193 -RRB- denotes )
T4909 8193-8194 . denotes .
T4910 8194-8371 sentence denotes Histological analysis revealed an extensive hyperplasia of brain tissue with herniation of brain tissue either through the skull-cap or through the ventral skull (Figure 3d,j).
T4911 8195-8207 JJ denotes Histological
T4912 8208-8216 NN denotes analysis
T4913 8217-8225 VBD denotes revealed
T4914 8226-8228 DT denotes an
T4916 8229-8238 JJ denotes extensive
T4915 8239-8250 NN denotes hyperplasia
T4917 8251-8253 IN denotes of
T4918 8254-8259 NN denotes brain
T4919 8260-8266 NN denotes tissue
T4920 8267-8271 IN denotes with
T4921 8272-8282 NN denotes herniation
T4922 8283-8285 IN denotes of
T4923 8286-8291 NN denotes brain
T4924 8292-8298 NN denotes tissue
T4925 8299-8305 CC denotes either
T4926 8306-8313 IN denotes through
T4927 8314-8317 DT denotes the
T4929 8318-8323 NN denotes skull
T4930 8323-8324 HYPH denotes -
T4928 8324-8327 NN denotes cap
T4931 8328-8330 CC denotes or
T4932 8331-8338 IN denotes through
T4933 8339-8342 DT denotes the
T4935 8343-8350 JJ denotes ventral
T4934 8351-8356 NN denotes skull
T4936 8357-8358 -LRB- denotes (
T4938 8358-8364 NN denotes Figure
T4939 8365-8367 NN denotes 3d
T4940 8367-8368 , denotes ,
T4937 8368-8369 NN denotes j
T4941 8369-8370 -RRB- denotes )
T4942 8370-8371 . denotes .
T4943 8371-8510 sentence denotes In the most severe cases, expansion of brain tissue in mutant mice resulted in further perturbations of cortical structures (Figure 3d,j).
T4944 8372-8374 IN denotes In
T4946 8375-8378 DT denotes the
T4948 8379-8383 RBS denotes most
T4949 8384-8390 JJ denotes severe
T4947 8391-8396 NNS denotes cases
T4950 8396-8398 , denotes ,
T4951 8398-8407 NN denotes expansion
T4952 8408-8410 IN denotes of
T4953 8411-8416 NN denotes brain
T4954 8417-8423 NN denotes tissue
T4955 8424-8426 IN denotes in
T4956 8427-8433 JJ denotes mutant
T4957 8434-8438 NNS denotes mice
T4945 8439-8447 VBD denotes resulted
T4958 8448-8450 IN denotes in
T4959 8451-8458 JJ denotes further
T4960 8459-8472 NNS denotes perturbations
T4961 8473-8475 IN denotes of
T4962 8476-8484 JJ denotes cortical
T4963 8485-8495 NNS denotes structures
T4964 8496-8497 -LRB- denotes (
T4966 8497-8503 NN denotes Figure
T4967 8504-8506 NN denotes 3d
T4968 8506-8507 , denotes ,
T4965 8507-8508 NN denotes j
T4969 8508-8509 -RRB- denotes )
T4970 8509-8510 . denotes .
T4971 8510-8629 sentence denotes Of note, a similar brain phenotype was observed in the Ptdsr-deficient mouse line generated by Li and colleagues [31].
T4972 8511-8513 IN denotes Of
T4974 8514-8518 NN denotes note
T4975 8518-8520 , denotes ,
T4976 8520-8521 DT denotes a
T4978 8522-8529 JJ denotes similar
T4979 8530-8535 NN denotes brain
T4977 8536-8545 NN denotes phenotype
T4980 8546-8549 VBD denotes was
T4973 8550-8558 VBN denotes observed
T4981 8559-8561 IN denotes in
T4982 8562-8565 DT denotes the
T4984 8566-8571 NN denotes Ptdsr
T4986 8571-8572 HYPH denotes -
T4985 8572-8581 JJ denotes deficient
T4987 8582-8587 NN denotes mouse
T4983 8588-8592 NN denotes line
T4988 8593-8602 VBN denotes generated
T4989 8603-8605 IN denotes by
T4990 8606-8608 NNP denotes Li
T4991 8609-8612 CC denotes and
T4992 8613-8623 NNS denotes colleagues
T4993 8624-8625 -LRB- denotes [
T4994 8625-8627 CD denotes 31
T4995 8627-8628 -RRB- denotes ]
T4996 8628-8629 . denotes .
T4997 8629-8733 sentence denotes In contrast to the study of Li et al. [31], however, we found almost normally developed lungs at birth.
T4998 8630-8632 IN denotes In
T5000 8633-8641 NN denotes contrast
T5001 8642-8644 IN denotes to
T5002 8645-8648 DT denotes the
T5003 8649-8654 NN denotes study
T5004 8655-8657 IN denotes of
T5005 8658-8660 NNP denotes Li
T5006 8661-8663 FW denotes et
T5007 8664-8667 FW denotes al.
T5008 8668-8669 -LRB- denotes [
T5009 8669-8671 CD denotes 31
T5010 8671-8672 -RRB- denotes ]
T5011 8672-8674 , denotes ,
T5012 8674-8681 RB denotes however
T5013 8681-8683 , denotes ,
T5014 8683-8685 PRP denotes we
T4999 8686-8691 VBD denotes found
T5015 8692-8698 RB denotes almost
T5017 8699-8707 RB denotes normally
T5016 8708-8717 VBN denotes developed
T5018 8718-8723 NNS denotes lungs
T5019 8724-8726 IN denotes at
T5020 8727-8732 NN denotes birth
T5021 8732-8733 . denotes .
T5022 8733-8885 sentence denotes Ptdsr -/-lungs showed, in comparison to wild-type, only a slight delay in maturation and were fully ventilated in neonates in most cases (Figure 3e,k).
T5023 8734-8739 NN denotes Ptdsr
T5025 8740-8741 SYM denotes -
T5026 8741-8742 HYPH denotes /
T5027 8742-8743 SYM denotes -
T5024 8743-8748 NNS denotes lungs
T5028 8749-8755 VBD denotes showed
T5029 8755-8757 , denotes ,
T5030 8757-8759 IN denotes in
T5031 8760-8770 NN denotes comparison
T5032 8771-8773 IN denotes to
T5033 8774-8778 JJ denotes wild
T5035 8778-8779 HYPH denotes -
T5034 8779-8783 NN denotes type
T5036 8783-8785 , denotes ,
T5037 8785-8789 RB denotes only
T5039 8790-8791 DT denotes a
T5040 8792-8798 JJ denotes slight
T5038 8799-8804 NN denotes delay
T5041 8805-8807 IN denotes in
T5042 8808-8818 NN denotes maturation
T5043 8819-8822 CC denotes and
T5044 8823-8827 VBD denotes were
T5045 8828-8833 RB denotes fully
T5046 8834-8844 JJ denotes ventilated
T5047 8845-8847 IN denotes in
T5048 8848-8856 NNS denotes neonates
T5049 8857-8859 IN denotes in
T5050 8860-8864 JJS denotes most
T5051 8865-8870 NNS denotes cases
T5052 8871-8872 -LRB- denotes (
T5054 8872-8878 NN denotes Figure
T5055 8879-8881 NN denotes 3e
T5056 8881-8882 , denotes ,
T5053 8882-8883 NN denotes k
T5057 8883-8884 -RRB- denotes )
T5058 8884-8885 . denotes .
T5059 8885-9031 sentence denotes This demonstrates that Ptdsr-deficient mice can overcome the delay in embryonic lung differentiation and display normal lung morphology at birth.
T5060 8886-8890 DT denotes This
T5061 8891-8903 VBZ denotes demonstrates
T5062 8904-8908 IN denotes that
T5064 8909-8914 NN denotes Ptdsr
T5066 8914-8915 HYPH denotes -
T5065 8915-8924 JJ denotes deficient
T5067 8925-8929 NNS denotes mice
T5068 8930-8933 MD denotes can
T5063 8934-8942 VB denotes overcome
T5069 8943-8946 DT denotes the
T5070 8947-8952 NN denotes delay
T5071 8953-8955 IN denotes in
T5072 8956-8965 JJ denotes embryonic
T5074 8966-8970 NN denotes lung
T5073 8971-8986 NN denotes differentiation
T5075 8987-8990 CC denotes and
T5076 8991-8998 VB denotes display
T5077 8999-9005 JJ denotes normal
T5079 9006-9010 NN denotes lung
T5078 9011-9021 NN denotes morphology
T5080 9022-9024 IN denotes at
T5081 9025-9030 NN denotes birth
T5082 9030-9031 . denotes .
T5083 9031-9119 sentence denotes Thus, it would appear highly unlikely that Ptdsr -/- mice die from respiratory failure.
T5084 9032-9036 RB denotes Thus
T5086 9036-9038 , denotes ,
T5087 9038-9040 PRP denotes it
T5088 9041-9046 MD denotes would
T5085 9047-9053 VB denotes appear
T5089 9054-9060 RB denotes highly
T5090 9061-9069 JJ denotes unlikely
T5091 9070-9074 IN denotes that
T5093 9075-9080 NN denotes Ptdsr
T5095 9081-9082 SYM denotes -
T5096 9082-9083 HYPH denotes /
T5097 9083-9084 SYM denotes -
T5094 9085-9089 NNS denotes mice
T5092 9090-9093 VBP denotes die
T5098 9094-9098 IN denotes from
T5099 9099-9110 JJ denotes respiratory
T5100 9111-9118 NN denotes failure
T5101 9118-9119 . denotes .
T5102 9119-9402 sentence denotes Consistent with the observations of Kunisaki and colleagues [32], we found severely blocked erythropoietic differentiation at an early erythroblast stage in the liver (Figure 3f,3l), suggesting an explanation for the grossly anemic appearance that we observed in our Ptdsr -/- mice.
T5103 9120-9130 JJ denotes Consistent
T5105 9131-9135 IN denotes with
T5106 9136-9139 DT denotes the
T5107 9140-9152 NNS denotes observations
T5108 9153-9155 IN denotes of
T5109 9156-9164 NNP denotes Kunisaki
T5110 9165-9168 CC denotes and
T5111 9169-9179 NNS denotes colleagues
T5112 9180-9181 -LRB- denotes [
T5113 9181-9183 CD denotes 32
T5114 9183-9184 -RRB- denotes ]
T5115 9184-9186 , denotes ,
T5116 9186-9188 PRP denotes we
T5104 9189-9194 VBD denotes found
T5117 9195-9203 RB denotes severely
T5118 9204-9211 VBN denotes blocked
T5120 9212-9226 JJ denotes erythropoietic
T5119 9227-9242 NN denotes differentiation
T5121 9243-9245 IN denotes at
T5122 9246-9248 DT denotes an
T5124 9249-9254 JJ denotes early
T5125 9255-9267 NN denotes erythroblast
T5123 9268-9273 NN denotes stage
T5126 9274-9276 IN denotes in
T5127 9277-9280 DT denotes the
T5128 9281-9286 NN denotes liver
T5129 9287-9288 -LRB- denotes (
T5131 9288-9294 NN denotes Figure
T5132 9295-9297 NN denotes 3f
T5133 9297-9298 , denotes ,
T5130 9298-9300 NN denotes 3l
T5134 9300-9301 -RRB- denotes )
T5135 9301-9303 , denotes ,
T5136 9303-9313 VBG denotes suggesting
T5137 9314-9316 DT denotes an
T5138 9317-9328 NN denotes explanation
T5139 9329-9332 IN denotes for
T5140 9333-9336 DT denotes the
T5142 9337-9344 RB denotes grossly
T5143 9345-9351 JJ denotes anemic
T5141 9352-9362 NN denotes appearance
T5144 9363-9367 WDT denotes that
T5146 9368-9370 PRP denotes we
T5145 9371-9379 VBD denotes observed
T5147 9380-9382 IN denotes in
T5148 9383-9386 PRP$ denotes our
T5150 9387-9392 NN denotes Ptdsr
T5151 9393-9394 SYM denotes -
T5152 9394-9395 HYPH denotes /
T5153 9395-9396 SYM denotes -
T5149 9397-9401 NNS denotes mice
T5154 9401-9402 . denotes .
T5402 9404-9408 NN denotes Loss
T5404 9409-9411 IN denotes of
T5405 9412-9417 NN denotes Ptdsr
T5406 9418-9426 NN denotes activity
T5407 9427-9429 VBZ denotes is
T5403 9430-9440 VBN denotes associated
T5408 9441-9445 IN denotes with
T5409 9446-9453 NNS denotes defects
T5410 9454-9456 IN denotes in
T5411 9457-9463 JJ denotes ocular
T5412 9464-9475 NN denotes development
T5413 9476-9479 CC denotes and
T5414 9480-9483 MD denotes can
T5415 9484-9488 VB denotes lead
T5416 9489-9491 IN denotes to
T5417 9492-9501 NN denotes formation
T5418 9502-9504 IN denotes of
T5419 9505-9512 JJ denotes ectopic
T5421 9513-9516 NN denotes eye
T5420 9517-9527 NNS denotes structures
T5422 9527-9756 sentence denotes By gross morphology we could differentiate two classes of Ptdsr mutants: those that appeared normal with both eyes present (Figure 4) and those that were severely affected and displayed uni- or bilateral anophthalmia (Figure 5).
T5423 9528-9530 IN denotes By
T5425 9531-9536 JJ denotes gross
T5426 9537-9547 NN denotes morphology
T5427 9548-9550 PRP denotes we
T5428 9551-9556 MD denotes could
T5424 9557-9570 VB denotes differentiate
T5429 9571-9574 CD denotes two
T5430 9575-9582 NNS denotes classes
T5431 9583-9585 IN denotes of
T5432 9586-9591 NN denotes Ptdsr
T5433 9592-9599 NNS denotes mutants
T5434 9599-9601 : denotes :
T5435 9601-9606 DT denotes those
T5436 9607-9611 WDT denotes that
T5437 9612-9620 VBD denotes appeared
T5438 9621-9627 JJ denotes normal
T5439 9628-9632 IN denotes with
T5440 9633-9637 DT denotes both
T5441 9638-9642 NNS denotes eyes
T5442 9643-9650 JJ denotes present
T5443 9651-9652 -LRB- denotes (
T5444 9652-9658 NN denotes Figure
T5445 9659-9660 CD denotes 4
T5446 9660-9661 -RRB- denotes )
T5447 9662-9665 CC denotes and
T5448 9666-9671 DT denotes those
T5449 9672-9676 WDT denotes that
T5451 9677-9681 VBD denotes were
T5452 9682-9690 RB denotes severely
T5450 9691-9699 VBN denotes affected
T5453 9700-9703 CC denotes and
T5454 9704-9713 VBN denotes displayed
T5455 9714-9717 JJ denotes uni
T5457 9717-9718 HYPH denotes -
T5458 9719-9721 CC denotes or
T5459 9722-9731 JJ denotes bilateral
T5456 9732-9744 NN denotes anophthalmia
T5460 9745-9746 -LRB- denotes (
T5461 9746-9752 NN denotes Figure
T5462 9753-9754 CD denotes 5
T5463 9754-9755 -RRB- denotes )
T5464 9755-9756 . denotes .
T5465 9756-9921 sentence denotes Analysis of normal or mildly affected embryos revealed no differences between mutant and wild-type embryos in the differentiation of the developing eye until E16.5.
T5466 9757-9765 NN denotes Analysis
T5468 9766-9768 IN denotes of
T5469 9769-9775 JJ denotes normal
T5471 9776-9778 CC denotes or
T5472 9779-9785 RB denotes mildly
T5473 9786-9794 VBN denotes affected
T5470 9795-9802 NNS denotes embryos
T5467 9803-9811 VBD denotes revealed
T5474 9812-9814 DT denotes no
T5475 9815-9826 NNS denotes differences
T5476 9827-9834 IN denotes between
T5477 9835-9841 JJ denotes mutant
T5479 9842-9845 CC denotes and
T5480 9846-9850 JJ denotes wild
T5482 9850-9851 HYPH denotes -
T5481 9851-9855 NN denotes type
T5478 9856-9863 NNS denotes embryos
T5483 9864-9866 IN denotes in
T5484 9867-9870 DT denotes the
T5485 9871-9886 NN denotes differentiation
T5486 9887-9889 IN denotes of
T5487 9890-9893 DT denotes the
T5489 9894-9904 VBG denotes developing
T5488 9905-9908 NN denotes eye
T5490 9909-9914 IN denotes until
T5491 9915-9920 NN denotes E16.5
T5492 9920-9921 . denotes .
T5493 9921-10071 sentence denotes In both genotypes, inner and outer layers of the retina displayed a comparable differentiation status, as shown, for example, at E12.5 (Figure 4a,e).
T5494 9922-9924 IN denotes In
T5496 9925-9929 DT denotes both
T5497 9930-9939 NNS denotes genotypes
T5498 9939-9941 , denotes ,
T5499 9941-9946 JJ denotes inner
T5501 9947-9950 CC denotes and
T5502 9951-9956 JJ denotes outer
T5500 9957-9963 NNS denotes layers
T5503 9964-9966 IN denotes of
T5504 9967-9970 DT denotes the
T5505 9971-9977 NN denotes retina
T5495 9978-9987 VBD denotes displayed
T5506 9988-9989 DT denotes a
T5508 9990-10000 JJ denotes comparable
T5509 10001-10016 NN denotes differentiation
T5507 10017-10023 NN denotes status
T5510 10023-10025 , denotes ,
T5511 10025-10027 IN denotes as
T5512 10028-10033 VBN denotes shown
T5513 10033-10035 , denotes ,
T5514 10035-10038 IN denotes for
T5515 10039-10046 NN denotes example
T5516 10046-10048 , denotes ,
T5517 10048-10050 IN denotes at
T5518 10051-10056 NN denotes E12.5
T5519 10057-10058 -LRB- denotes (
T5521 10058-10064 NN denotes Figure
T5522 10065-10067 NN denotes 4a
T5523 10067-10068 , denotes ,
T5520 10068-10069 NN denotes e
T5524 10069-10070 -RRB- denotes )
T5525 10070-10071 . denotes .
T5526 10071-10245 sentence denotes At day E16.5, however, retinal layers in Ptdsr -/- embryos were much thinner than in wild-type embryos, contained fewer cells and were greatly reduced in size (Figure 4b,f).
T5527 10072-10074 IN denotes At
T5529 10075-10078 NN denotes day
T5530 10079-10084 NN denotes E16.5
T5531 10084-10086 , denotes ,
T5532 10086-10093 RB denotes however
T5533 10093-10095 , denotes ,
T5534 10095-10102 JJ denotes retinal
T5535 10103-10109 NNS denotes layers
T5536 10110-10112 IN denotes in
T5537 10113-10118 NN denotes Ptdsr
T5539 10119-10120 SYM denotes -
T5540 10120-10121 HYPH denotes /
T5541 10121-10122 SYM denotes -
T5538 10123-10130 NNS denotes embryos
T5528 10131-10135 VBD denotes were
T5542 10136-10140 RB denotes much
T5543 10141-10148 JJR denotes thinner
T5544 10149-10153 IN denotes than
T5545 10154-10156 IN denotes in
T5546 10157-10161 JJ denotes wild
T5548 10161-10162 HYPH denotes -
T5547 10162-10166 NN denotes type
T5549 10167-10174 NNS denotes embryos
T5550 10174-10176 , denotes ,
T5551 10176-10185 VBD denotes contained
T5552 10186-10191 JJR denotes fewer
T5553 10192-10197 NNS denotes cells
T5554 10198-10201 CC denotes and
T5555 10202-10206 VBD denotes were
T5557 10207-10214 RB denotes greatly
T5556 10215-10222 VBN denotes reduced
T5558 10223-10225 IN denotes in
T5559 10226-10230 NN denotes size
T5560 10231-10232 -LRB- denotes (
T5562 10232-10238 NN denotes Figure
T5563 10239-10241 NN denotes 4b
T5564 10241-10242 , denotes ,
T5561 10242-10243 NN denotes f
T5565 10243-10244 -RRB- denotes )
T5566 10244-10245 . denotes .
T5567 10245-10415 sentence denotes Comparison of the retinal structures of Ptdsr +/+ and Ptdsr -/- embryos revealed that all four retinal layers were present in Ptdsr-knockout mice at E16.5 (Figure 4b,f).
T5568 10246-10256 NN denotes Comparison
T5570 10257-10259 IN denotes of
T5571 10260-10263 DT denotes the
T5573 10264-10271 JJ denotes retinal
T5572 10272-10282 NNS denotes structures
T5574 10283-10285 IN denotes of
T5575 10286-10291 NN denotes Ptdsr
T5577 10292-10293 SYM denotes +
T5578 10293-10294 HYPH denotes /
T5579 10294-10295 SYM denotes +
T5580 10296-10299 CC denotes and
T5581 10300-10305 NN denotes Ptdsr
T5582 10306-10307 SYM denotes -
T5583 10307-10308 HYPH denotes /
T5584 10308-10309 SYM denotes -
T5576 10310-10317 NNS denotes embryos
T5569 10318-10326 VBD denotes revealed
T5585 10327-10331 IN denotes that
T5587 10332-10335 DT denotes all
T5589 10336-10340 CD denotes four
T5590 10341-10348 JJ denotes retinal
T5588 10349-10355 NNS denotes layers
T5586 10356-10360 VBD denotes were
T5591 10361-10368 JJ denotes present
T5592 10369-10371 IN denotes in
T5593 10372-10377 NN denotes Ptdsr
T5595 10377-10378 HYPH denotes -
T5594 10378-10386 NN denotes knockout
T5596 10387-10391 NNS denotes mice
T5597 10392-10394 IN denotes at
T5598 10395-10400 NN denotes E16.5
T5599 10401-10402 -LRB- denotes (
T5601 10402-10408 NN denotes Figure
T5602 10409-10411 NN denotes 4b
T5603 10411-10412 , denotes ,
T5600 10412-10413 NN denotes f
T5604 10413-10414 -RRB- denotes )
T5605 10414-10415 . denotes .
T5606 10415-10681 sentence denotes At E18.5 (Figure 4c,g) and in neonatal animals (postnatal day P0; Figure 4d,h), the differences in retinal differentiation between Ptdsr+/+ and Ptdsr -/- mice were still evident, but the size reduction of the retinal layers was less pronounced in the knockout mice.
T5607 10416-10418 IN denotes At
T5609 10419-10424 NN denotes E18.5
T5610 10425-10426 -LRB- denotes (
T5612 10426-10432 NN denotes Figure
T5613 10433-10435 NN denotes 4c
T5614 10435-10436 , denotes ,
T5611 10436-10437 NN denotes g
T5615 10437-10438 -RRB- denotes )
T5616 10439-10442 CC denotes and
T5617 10443-10445 IN denotes in
T5618 10446-10454 JJ denotes neonatal
T5619 10455-10462 NNS denotes animals
T5620 10463-10464 -LRB- denotes (
T5622 10464-10473 JJ denotes postnatal
T5624 10474-10477 NN denotes day
T5623 10478-10480 NN denotes P0
T5625 10480-10481 : denotes ;
T5626 10482-10488 NN denotes Figure
T5627 10489-10491 NN denotes 4d
T5628 10491-10492 , denotes ,
T5621 10492-10493 NN denotes h
T5629 10493-10494 -RRB- denotes )
T5630 10494-10496 , denotes ,
T5631 10496-10499 DT denotes the
T5632 10500-10511 NNS denotes differences
T5633 10512-10514 IN denotes in
T5634 10515-10522 JJ denotes retinal
T5635 10523-10538 NN denotes differentiation
T5636 10539-10546 IN denotes between
T5637 10547-10552 NN denotes Ptdsr
T5639 10552-10553 SYM denotes +
T5640 10553-10554 HYPH denotes /
T5641 10554-10555 SYM denotes +
T5642 10556-10559 CC denotes and
T5643 10560-10565 NN denotes Ptdsr
T5644 10566-10567 SYM denotes -
T5645 10567-10568 HYPH denotes /
T5646 10568-10569 SYM denotes -
T5638 10570-10574 NNS denotes mice
T5608 10575-10579 VBD denotes were
T5647 10580-10585 RB denotes still
T5648 10586-10593 JJ denotes evident
T5649 10593-10595 , denotes ,
T5650 10595-10598 CC denotes but
T5651 10599-10602 DT denotes the
T5653 10603-10607 NN denotes size
T5652 10608-10617 NN denotes reduction
T5655 10618-10620 IN denotes of
T5656 10621-10624 DT denotes the
T5658 10625-10632 JJ denotes retinal
T5657 10633-10639 NNS denotes layers
T5654 10640-10643 VBD denotes was
T5659 10644-10648 RBR denotes less
T5660 10649-10659 JJ denotes pronounced
T5661 10660-10662 IN denotes in
T5662 10663-10666 DT denotes the
T5664 10667-10675 NN denotes knockout
T5663 10676-10680 NNS denotes mice
T5665 10680-10681 . denotes .
T5666 10681-10812 sentence denotes Ptdsr-deficient animals seem to have compensated for the marked delay in cellular differentiation and expansion of retinal layers.
T5667 10682-10687 NN denotes Ptdsr
T5669 10687-10688 HYPH denotes -
T5668 10688-10697 JJ denotes deficient
T5670 10698-10705 NNS denotes animals
T5671 10706-10710 VBP denotes seem
T5672 10711-10713 TO denotes to
T5674 10714-10718 VB denotes have
T5673 10719-10730 VBN denotes compensated
T5675 10731-10734 IN denotes for
T5676 10735-10738 DT denotes the
T5678 10739-10745 JJ denotes marked
T5677 10746-10751 NN denotes delay
T5679 10752-10754 IN denotes in
T5680 10755-10763 JJ denotes cellular
T5681 10764-10779 NN denotes differentiation
T5682 10780-10783 CC denotes and
T5683 10784-10793 NN denotes expansion
T5684 10794-10796 IN denotes of
T5685 10797-10804 JJ denotes retinal
T5686 10805-10811 NNS denotes layers
T5687 10811-10812 . denotes .
T5688 10812-11120 sentence denotes Close examination of retinal structures revealed that the inner granular layer was still less expanded in Ptdsr-deficient animals, however, and that it contained fewer cells and was still severely underdeveloped in comparison with the corresponding retinal layer in control animals (Figure 4c,4g and 4d,4h).
T5689 10813-10818 NN denotes Close
T5690 10819-10830 NN denotes examination
T5692 10831-10833 IN denotes of
T5693 10834-10841 JJ denotes retinal
T5694 10842-10852 NNS denotes structures
T5691 10853-10861 VBD denotes revealed
T5695 10862-10866 IN denotes that
T5697 10867-10870 DT denotes the
T5699 10871-10876 JJ denotes inner
T5700 10877-10885 JJ denotes granular
T5698 10886-10891 NN denotes layer
T5701 10892-10895 VBD denotes was
T5702 10896-10901 RB denotes still
T5703 10902-10906 RBR denotes less
T5696 10907-10915 VBN denotes expanded
T5704 10916-10918 IN denotes in
T5705 10919-10924 NN denotes Ptdsr
T5707 10924-10925 HYPH denotes -
T5706 10925-10934 JJ denotes deficient
T5708 10935-10942 NNS denotes animals
T5709 10942-10944 , denotes ,
T5710 10944-10951 RB denotes however
T5711 10951-10953 , denotes ,
T5712 10953-10956 CC denotes and
T5713 10957-10961 IN denotes that
T5715 10962-10964 PRP denotes it
T5714 10965-10974 VBD denotes contained
T5716 10975-10980 JJR denotes fewer
T5717 10981-10986 NNS denotes cells
T5718 10987-10990 CC denotes and
T5719 10991-10994 VBD denotes was
T5720 10995-11000 RB denotes still
T5721 11001-11009 RB denotes severely
T5722 11010-11024 JJ denotes underdeveloped
T5723 11025-11027 IN denotes in
T5724 11028-11038 NN denotes comparison
T5725 11039-11043 IN denotes with
T5726 11044-11047 DT denotes the
T5728 11048-11061 VBG denotes corresponding
T5729 11062-11069 JJ denotes retinal
T5727 11070-11075 NN denotes layer
T5730 11076-11078 IN denotes in
T5731 11079-11086 NN denotes control
T5732 11087-11094 NNS denotes animals
T5733 11095-11096 -LRB- denotes (
T5735 11096-11102 NNP denotes Figure
T5734 11103-11105 NN denotes 4c
T5736 11105-11106 , denotes ,
T5737 11106-11108 NN denotes 4g
T5738 11109-11112 CC denotes and
T5739 11113-11115 NN denotes 4d
T5740 11115-11116 , denotes ,
T5741 11116-11118 NN denotes 4h
T5742 11118-11119 -RRB- denotes )
T5743 11119-11120 . denotes .
T5744 11120-11245 sentence denotes Thus, even mildly affected Ptdsr -/- mutants had ocular malformations with defects in differentiation of retinal structures.
T5745 11121-11125 RB denotes Thus
T5747 11125-11127 , denotes ,
T5748 11127-11131 RB denotes even
T5749 11132-11138 RB denotes mildly
T5750 11139-11147 VBN denotes affected
T5752 11148-11153 NN denotes Ptdsr
T5753 11154-11155 SYM denotes -
T5754 11155-11156 HYPH denotes /
T5755 11156-11157 SYM denotes -
T5751 11158-11165 NNS denotes mutants
T5746 11166-11169 VBD denotes had
T5756 11170-11176 JJ denotes ocular
T5757 11177-11190 NNS denotes malformations
T5758 11191-11195 IN denotes with
T5759 11196-11203 NNS denotes defects
T5760 11204-11206 IN denotes in
T5761 11207-11222 NN denotes differentiation
T5762 11223-11225 IN denotes of
T5763 11226-11233 JJ denotes retinal
T5764 11234-11244 NNS denotes structures
T5765 11244-11245 . denotes .
T5766 11245-11386 sentence denotes We next examined Ptdsr -/- embryos that displayed unilateral or bilateral absence of eyes (Figure 5a) by serial sectioning of whole embryos.
T5767 11246-11248 PRP denotes We
T5769 11249-11253 RB denotes next
T5768 11254-11262 VBD denotes examined
T5770 11263-11268 NN denotes Ptdsr
T5772 11269-11270 SYM denotes -
T5773 11270-11271 HYPH denotes /
T5774 11271-11272 SYM denotes -
T5771 11273-11280 NNS denotes embryos
T5775 11281-11285 WDT denotes that
T5776 11286-11295 VBD denotes displayed
T5777 11296-11306 JJ denotes unilateral
T5779 11307-11309 CC denotes or
T5780 11310-11319 JJ denotes bilateral
T5778 11320-11327 NN denotes absence
T5781 11328-11330 IN denotes of
T5782 11331-11335 NNS denotes eyes
T5783 11336-11337 -LRB- denotes (
T5785 11337-11343 NN denotes Figure
T5784 11344-11346 NN denotes 5a
T5786 11346-11347 -RRB- denotes )
T5787 11348-11350 IN denotes by
T5788 11351-11357 JJ denotes serial
T5789 11358-11368 NN denotes sectioning
T5790 11369-11371 IN denotes of
T5791 11372-11377 JJ denotes whole
T5792 11378-11385 NNS denotes embryos
T5793 11385-11386 . denotes .
T5794 11386-11492 sentence denotes These embryos showed complex malformations of the optical cup, including absence of the lens (Figure 5b).
T5795 11387-11392 DT denotes These
T5796 11393-11400 NNS denotes embryos
T5797 11401-11407 VBD denotes showed
T5798 11408-11415 JJ denotes complex
T5799 11416-11429 NNS denotes malformations
T5800 11430-11432 IN denotes of
T5801 11433-11436 DT denotes the
T5803 11437-11444 JJ denotes optical
T5802 11445-11448 NN denotes cup
T5804 11448-11450 , denotes ,
T5805 11450-11459 VBG denotes including
T5806 11460-11467 NN denotes absence
T5807 11468-11470 IN denotes of
T5808 11471-11474 DT denotes the
T5809 11475-11479 NN denotes lens
T5810 11480-11481 -LRB- denotes (
T5812 11481-11487 NN denotes Figure
T5811 11488-11490 NN denotes 5b
T5813 11490-11491 -RRB- denotes )
T5814 11491-11492 . denotes .
T5815 11492-11652 sentence denotes Most surprisingly, we found pigmented epithelial cells in the nasal cavity of all Ptdsr-knockout mice with anophthalmia that were analyzed histopathologically.
T5816 11493-11497 RBS denotes Most
T5817 11498-11510 RB denotes surprisingly
T5819 11510-11512 , denotes ,
T5820 11512-11514 PRP denotes we
T5818 11515-11520 VBD denotes found
T5821 11521-11530 VBN denotes pigmented
T5823 11531-11541 JJ denotes epithelial
T5822 11542-11547 NNS denotes cells
T5824 11548-11550 IN denotes in
T5825 11551-11554 DT denotes the
T5827 11555-11560 JJ denotes nasal
T5826 11561-11567 NN denotes cavity
T5828 11568-11570 IN denotes of
T5829 11571-11574 DT denotes all
T5831 11575-11580 NN denotes Ptdsr
T5833 11580-11581 HYPH denotes -
T5832 11581-11589 NN denotes knockout
T5830 11590-11594 NNS denotes mice
T5834 11595-11599 IN denotes with
T5835 11600-11612 NN denotes anophthalmia
T5836 11613-11617 WDT denotes that
T5838 11618-11622 VBD denotes were
T5837 11623-11631 VBN denotes analyzed
T5839 11632-11651 RB denotes histopathologically
T5840 11651-11652 . denotes .
T5841 11652-11821 sentence denotes We could identify black-colored pigmented cells embedded in the epithelium of the maxillary sinus that resembled presumptive retinal-pigmented epithelium (Figure 5b,c).
T5842 11653-11655 PRP denotes We
T5844 11656-11661 MD denotes could
T5843 11662-11670 VB denotes identify
T5845 11671-11676 JJ denotes black
T5847 11676-11677 HYPH denotes -
T5846 11677-11684 VBN denotes colored
T5849 11685-11694 VBN denotes pigmented
T5848 11695-11700 NNS denotes cells
T5850 11701-11709 VBN denotes embedded
T5851 11710-11712 IN denotes in
T5852 11713-11716 DT denotes the
T5853 11717-11727 NN denotes epithelium
T5854 11728-11730 IN denotes of
T5855 11731-11734 DT denotes the
T5857 11735-11744 JJ denotes maxillary
T5856 11745-11750 NN denotes sinus
T5858 11751-11755 WDT denotes that
T5859 11756-11765 VBD denotes resembled
T5860 11766-11777 JJ denotes presumptive
T5862 11778-11785 JJ denotes retinal
T5864 11785-11786 HYPH denotes -
T5863 11786-11795 VBN denotes pigmented
T5861 11796-11806 NN denotes epithelium
T5865 11807-11808 -LRB- denotes (
T5867 11808-11814 NN denotes Figure
T5868 11815-11817 NN denotes 5b
T5869 11817-11818 , denotes ,
T5866 11818-11819 NN denotes c
T5870 11819-11820 -RRB- denotes )
T5871 11820-11821 . denotes .
T5872 11821-12063 sentence denotes Examination of consecutive serial sections revealed the formation of a primitive eye structure, with induction and subsequent proliferation of ectopic mesenchymal tissue immediately adjacent to the displaced pigmented epithelium (Figure 5d).
T5873 11822-11833 NN denotes Examination
T5875 11834-11836 IN denotes of
T5876 11837-11848 JJ denotes consecutive
T5878 11849-11855 JJ denotes serial
T5877 11856-11864 NNS denotes sections
T5874 11865-11873 VBD denotes revealed
T5879 11874-11877 DT denotes the
T5880 11878-11887 NN denotes formation
T5881 11888-11890 IN denotes of
T5882 11891-11892 DT denotes a
T5884 11893-11902 JJ denotes primitive
T5885 11903-11906 NN denotes eye
T5883 11907-11916 NN denotes structure
T5886 11916-11918 , denotes ,
T5887 11918-11922 IN denotes with
T5888 11923-11932 NN denotes induction
T5889 11933-11936 CC denotes and
T5890 11937-11947 JJ denotes subsequent
T5891 11948-11961 NN denotes proliferation
T5892 11962-11964 IN denotes of
T5893 11965-11972 JJ denotes ectopic
T5895 11973-11984 JJ denotes mesenchymal
T5894 11985-11991 NN denotes tissue
T5896 11992-12003 RB denotes immediately
T5897 12004-12012 JJ denotes adjacent
T5898 12013-12015 IN denotes to
T5899 12016-12019 DT denotes the
T5901 12020-12029 VBN denotes displaced
T5902 12030-12039 VBN denotes pigmented
T5900 12040-12050 NN denotes epithelium
T5903 12051-12052 -LRB- denotes (
T5905 12052-12058 NN denotes Figure
T5904 12059-12061 NN denotes 5d
T5906 12061-12062 -RRB- denotes )
T5907 12062-12063 . denotes .
T5908 12063-12186 sentence denotes This structure was clearly induced ectopically, and we failed to identify similar changes in any of the wild-type embryos.
T5909 12064-12068 DT denotes This
T5910 12069-12078 NN denotes structure
T5912 12079-12082 VBD denotes was
T5913 12083-12090 RB denotes clearly
T5911 12091-12098 VBN denotes induced
T5914 12099-12110 RB denotes ectopically
T5915 12110-12112 , denotes ,
T5916 12112-12115 CC denotes and
T5917 12116-12118 PRP denotes we
T5918 12119-12125 VBD denotes failed
T5919 12126-12128 TO denotes to
T5920 12129-12137 VB denotes identify
T5921 12138-12145 JJ denotes similar
T5922 12146-12153 NNS denotes changes
T5923 12154-12156 IN denotes in
T5924 12157-12160 DT denotes any
T5925 12161-12163 IN denotes of
T5926 12164-12167 DT denotes the
T5928 12168-12172 JJ denotes wild
T5930 12172-12173 HYPH denotes -
T5929 12173-12177 NN denotes type
T5927 12178-12185 NNS denotes embryos
T5931 12185-12186 . denotes .
T5932 12186-12538 sentence denotes In summary, we observed a wide range of ocular malformations in Ptdsr-deficient mice that ranged from differentiation defects in retinal cell layers (for example, the inner granular layer) in mildly affected homozygotes to anophthalmia in severely affected Ptdsr -/- mice that was associated with induction of ectopic eye structures in nasal cavities.
T5933 12187-12189 IN denotes In
T5935 12190-12197 NN denotes summary
T5936 12197-12199 , denotes ,
T5937 12199-12201 PRP denotes we
T5934 12202-12210 VBD denotes observed
T5938 12211-12212 DT denotes a
T5940 12213-12217 JJ denotes wide
T5939 12218-12223 NN denotes range
T5941 12224-12226 IN denotes of
T5942 12227-12233 JJ denotes ocular
T5943 12234-12247 NNS denotes malformations
T5944 12248-12250 IN denotes in
T5945 12251-12256 NN denotes Ptdsr
T5947 12256-12257 HYPH denotes -
T5946 12257-12266 JJ denotes deficient
T5948 12267-12271 NNS denotes mice
T5949 12272-12276 WDT denotes that
T5950 12277-12283 VBD denotes ranged
T5951 12284-12288 IN denotes from
T5952 12289-12304 NN denotes differentiation
T5953 12305-12312 NNS denotes defects
T5954 12313-12315 IN denotes in
T5955 12316-12323 JJ denotes retinal
T5957 12324-12328 NN denotes cell
T5956 12329-12335 NNS denotes layers
T5958 12336-12337 -LRB- denotes (
T5960 12337-12340 IN denotes for
T5961 12341-12348 NN denotes example
T5962 12348-12350 , denotes ,
T5963 12350-12353 DT denotes the
T5964 12354-12359 JJ denotes inner
T5965 12360-12368 JJ denotes granular
T5959 12369-12374 NN denotes layer
T5966 12374-12375 -RRB- denotes )
T5967 12376-12378 IN denotes in
T5968 12379-12385 RB denotes mildly
T5969 12386-12394 VBN denotes affected
T5970 12395-12406 NNS denotes homozygotes
T5971 12407-12409 IN denotes to
T5972 12410-12422 NN denotes anophthalmia
T5973 12423-12425 IN denotes in
T5974 12426-12434 RB denotes severely
T5975 12435-12443 VBN denotes affected
T5977 12444-12449 NN denotes Ptdsr
T5978 12450-12451 SYM denotes -
T5979 12451-12452 HYPH denotes /
T5980 12452-12453 SYM denotes -
T5976 12454-12458 NNS denotes mice
T5981 12459-12463 WDT denotes that
T5983 12464-12467 VBD denotes was
T5982 12468-12478 VBN denotes associated
T5984 12479-12483 IN denotes with
T5985 12484-12493 NN denotes induction
T5986 12494-12496 IN denotes of
T5987 12497-12504 JJ denotes ectopic
T5989 12505-12508 NN denotes eye
T5988 12509-12519 NNS denotes structures
T5990 12520-12522 IN denotes in
T5991 12523-12528 JJ denotes nasal
T5992 12529-12537 NNS denotes cavities
T5993 12537-12538 . denotes .
T6746 12540-12552 NN denotes Phagocytosis
T6748 12553-12556 CC denotes and
T6749 12557-12566 NN denotes clearance
T6750 12567-12569 IN denotes of
T6751 12570-12579 JJ denotes apoptotic
T6752 12580-12585 NNS denotes cells
T6747 12586-12588 VBZ denotes is
T6753 12589-12595 JJ denotes normal
T6754 12596-12598 IN denotes in
T6755 12599-12604 NN denotes Ptdsr
T6757 12604-12605 HYPH denotes -
T6756 12605-12614 JJ denotes deficient
T6758 12615-12619 NNS denotes mice
T6759 12619-12711 sentence denotes We next tested whether Ptdsr is functionally required for the clearance of apoptotic cells.
T6760 12620-12622 PRP denotes We
T6762 12623-12627 RB denotes next
T6761 12628-12634 VBD denotes tested
T6763 12635-12642 IN denotes whether
T6765 12643-12648 NN denotes Ptdsr
T6766 12649-12651 VBZ denotes is
T6767 12652-12664 RB denotes functionally
T6764 12665-12673 VBN denotes required
T6768 12674-12677 IN denotes for
T6769 12678-12681 DT denotes the
T6770 12682-12691 NN denotes clearance
T6771 12692-12694 IN denotes of
T6772 12695-12704 JJ denotes apoptotic
T6773 12705-12710 NNS denotes cells
T6774 12710-12711 . denotes .
T6775 12711-12817 sentence denotes We started with an investigation of cell death in vivo in the interdigital areas of the developing limbs.
T6776 12712-12714 PRP denotes We
T6777 12715-12722 VBD denotes started
T6778 12723-12727 IN denotes with
T6779 12728-12730 DT denotes an
T6780 12731-12744 NN denotes investigation
T6781 12745-12747 IN denotes of
T6782 12748-12752 NN denotes cell
T6783 12753-12758 NN denotes death
T6784 12759-12761 FW denotes in
T6785 12762-12766 FW denotes vivo
T6786 12767-12769 IN denotes in
T6787 12770-12773 DT denotes the
T6789 12774-12786 JJ denotes interdigital
T6788 12787-12792 NNS denotes areas
T6790 12793-12795 IN denotes of
T6791 12796-12799 DT denotes the
T6793 12800-12810 VBG denotes developing
T6792 12811-12816 NNS denotes limbs
T6794 12816-12817 . denotes .
T6795 12817-13074 sentence denotes Apoptosis of interdigital cells in the distal mesenchyme of limb buds occurs most prominently from developmental stages E12.0 to E13.5 and can be easily examined in situ by whole-mount terminal deoxynucleotide transferase-mediated UTP end-labeling (TUNEL).
T6796 12818-12827 NN denotes Apoptosis
T6798 12828-12830 IN denotes of
T6799 12831-12843 JJ denotes interdigital
T6800 12844-12849 NNS denotes cells
T6801 12850-12852 IN denotes in
T6802 12853-12856 DT denotes the
T6804 12857-12863 JJ denotes distal
T6803 12864-12874 NN denotes mesenchyme
T6805 12875-12877 IN denotes of
T6806 12878-12882 NN denotes limb
T6807 12883-12887 NNS denotes buds
T6797 12888-12894 VBZ denotes occurs
T6808 12895-12899 RBS denotes most
T6809 12900-12911 RB denotes prominently
T6810 12912-12916 IN denotes from
T6811 12917-12930 JJ denotes developmental
T6813 12931-12937 NNS denotes stages
T6812 12938-12943 NN denotes E12.0
T6814 12944-12946 IN denotes to
T6815 12947-12952 NN denotes E13.5
T6816 12953-12956 CC denotes and
T6817 12957-12960 MD denotes can
T6819 12961-12963 VB denotes be
T6820 12964-12970 RB denotes easily
T6818 12971-12979 VBN denotes examined
T6821 12980-12982 FW denotes in
T6822 12983-12987 FW denotes situ
T6823 12988-12990 IN denotes by
T6824 12991-12996 JJ denotes whole
T6826 12996-12997 HYPH denotes -
T6825 12997-13002 NN denotes mount
T6828 13003-13011 JJ denotes terminal
T6829 13012-13027 NN denotes deoxynucleotide
T6830 13028-13039 NN denotes transferase
T6832 13039-13040 HYPH denotes -
T6831 13040-13048 VBN denotes mediated
T6833 13049-13052 NN denotes UTP
T6827 13053-13056 NN denotes end
T6834 13056-13057 HYPH denotes -
T6835 13057-13065 VBG denotes labeling
T6836 13066-13067 -LRB- denotes (
T6837 13067-13072 NN denotes TUNEL
T6838 13072-13073 -RRB- denotes )
T6839 13073-13074 . denotes .
T6840 13074-13222 sentence denotes We compared the pattern of interdigital cell death in fore and hind limb buds from Ptdsr -/- (n = 3) and Ptdsr +/+ (n = 3) mice at E12.5 and E13.5.
T6841 13075-13077 PRP denotes We
T6842 13078-13086 VBD denotes compared
T6843 13087-13090 DT denotes the
T6844 13091-13098 NN denotes pattern
T6845 13099-13101 IN denotes of
T6846 13102-13114 JJ denotes interdigital
T6848 13115-13119 NN denotes cell
T6847 13120-13125 NN denotes death
T6849 13126-13128 IN denotes in
T6850 13129-13133 JJ denotes fore
T6852 13134-13137 CC denotes and
T6853 13138-13142 JJ denotes hind
T6854 13143-13147 NN denotes limb
T6851 13148-13152 NNS denotes buds
T6855 13153-13157 IN denotes from
T6856 13158-13163 NN denotes Ptdsr
T6858 13164-13165 SYM denotes -
T6859 13165-13166 HYPH denotes /
T6860 13166-13167 SYM denotes -
T6861 13168-13169 -LRB- denotes (
T6863 13169-13170 NN denotes n
T6864 13171-13172 SYM denotes =
T6862 13173-13174 CD denotes 3
T6865 13174-13175 -RRB- denotes )
T6866 13176-13179 CC denotes and
T6867 13180-13185 NN denotes Ptdsr
T6868 13186-13187 SYM denotes +
T6869 13187-13188 HYPH denotes /
T6870 13188-13189 SYM denotes +
T6871 13190-13191 -LRB- denotes (
T6873 13191-13192 NN denotes n
T6874 13193-13194 SYM denotes =
T6872 13195-13196 CD denotes 3
T6875 13196-13197 -RRB- denotes )
T6857 13198-13202 NNS denotes mice
T6876 13203-13205 IN denotes at
T6877 13206-13211 NN denotes E12.5
T6878 13212-13215 CC denotes and
T6879 13216-13221 NN denotes E13.5
T6880 13221-13222 . denotes .
T6881 13222-13337 sentence denotes No differences in accumulation of TUNEL-positive cell corpses were observed between the two genotypes (Figure 6a).
T6882 13223-13225 DT denotes No
T6883 13226-13237 NNS denotes differences
T6885 13238-13240 IN denotes in
T6886 13241-13253 NN denotes accumulation
T6887 13254-13256 IN denotes of
T6888 13257-13262 NN denotes TUNEL
T6890 13262-13263 HYPH denotes -
T6889 13263-13271 JJ denotes positive
T6892 13272-13276 NN denotes cell
T6891 13277-13284 NNS denotes corpses
T6893 13285-13289 VBD denotes were
T6884 13290-13298 VBN denotes observed
T6894 13299-13306 IN denotes between
T6895 13307-13310 DT denotes the
T6897 13311-13314 CD denotes two
T6896 13315-13324 NNS denotes genotypes
T6898 13325-13326 -LRB- denotes (
T6900 13326-13332 NN denotes Figure
T6899 13333-13335 NN denotes 6a
T6901 13335-13336 -RRB- denotes )
T6902 13336-13337 . denotes .
T6903 13337-13602 sentence denotes The kinetics of cell death occurrence and regression of the interdigital web was similar in wild-type and mutant littermates, providing no evidence that Ptdsr-deficiency is associated with impaired clearance of apoptotic interdigital cells during limb development.
T6904 13338-13341 DT denotes The
T6905 13342-13350 NN denotes kinetics
T6907 13351-13353 IN denotes of
T6908 13354-13358 NN denotes cell
T6909 13359-13364 NN denotes death
T6910 13365-13375 NN denotes occurrence
T6911 13376-13379 CC denotes and
T6912 13380-13390 NN denotes regression
T6913 13391-13393 IN denotes of
T6914 13394-13397 DT denotes the
T6916 13398-13410 JJ denotes interdigital
T6915 13411-13414 NN denotes web
T6906 13415-13418 VBD denotes was
T6917 13419-13426 JJ denotes similar
T6918 13427-13429 IN denotes in
T6919 13430-13434 JJ denotes wild
T6921 13434-13435 HYPH denotes -
T6920 13435-13439 NN denotes type
T6923 13440-13443 CC denotes and
T6924 13444-13450 JJ denotes mutant
T6922 13451-13462 NNS denotes littermates
T6925 13462-13464 , denotes ,
T6926 13464-13473 VBG denotes providing
T6927 13474-13476 DT denotes no
T6928 13477-13485 NN denotes evidence
T6929 13486-13490 IN denotes that
T6931 13491-13496 NN denotes Ptdsr
T6933 13496-13497 HYPH denotes -
T6932 13497-13507 NN denotes deficiency
T6934 13508-13510 VBZ denotes is
T6930 13511-13521 VBN denotes associated
T6935 13522-13526 IN denotes with
T6936 13527-13535 JJ denotes impaired
T6937 13536-13545 NN denotes clearance
T6938 13546-13548 IN denotes of
T6939 13549-13558 JJ denotes apoptotic
T6941 13559-13571 JJ denotes interdigital
T6940 13572-13577 NNS denotes cells
T6942 13578-13584 IN denotes during
T6943 13585-13589 NN denotes limb
T6944 13590-13601 NN denotes development
T6945 13601-13602 . denotes .
T6946 13602-13878 sentence denotes To investigate further whether removal of apoptotic cells is impaired in Ptdsr -/- mice, we stained immunohistochemically for activated caspase 3 (aCasp3) and analyzed additional organs and tissues where apoptosis plays a crucial role in tissue remodeling during development.
T6947 13603-13605 TO denotes To
T6948 13606-13617 VB denotes investigate
T6950 13618-13625 RBR denotes further
T6951 13626-13633 IN denotes whether
T6953 13634-13641 NN denotes removal
T6954 13642-13644 IN denotes of
T6955 13645-13654 JJ denotes apoptotic
T6956 13655-13660 NNS denotes cells
T6957 13661-13663 VBZ denotes is
T6952 13664-13672 VBN denotes impaired
T6958 13673-13675 IN denotes in
T6959 13676-13681 NN denotes Ptdsr
T6961 13682-13683 SYM denotes -
T6962 13683-13684 HYPH denotes /
T6963 13684-13685 SYM denotes -
T6960 13686-13690 NNS denotes mice
T6964 13690-13692 , denotes ,
T6965 13692-13694 PRP denotes we
T6949 13695-13702 VBD denotes stained
T6966 13703-13724 RB denotes immunohistochemically
T6967 13725-13728 IN denotes for
T6968 13729-13738 VBN denotes activated
T6969 13739-13746 NN denotes caspase
T6970 13747-13748 CD denotes 3
T6971 13749-13750 -LRB- denotes (
T6972 13750-13756 NN denotes aCasp3
T6973 13756-13757 -RRB- denotes )
T6974 13758-13761 CC denotes and
T6975 13762-13770 VBN denotes analyzed
T6976 13771-13781 JJ denotes additional
T6977 13782-13788 NNS denotes organs
T6978 13789-13792 CC denotes and
T6979 13793-13800 NNS denotes tissues
T6980 13801-13806 WRB denotes where
T6982 13807-13816 NN denotes apoptosis
T6981 13817-13822 VBZ denotes plays
T6983 13823-13824 DT denotes a
T6985 13825-13832 JJ denotes crucial
T6984 13833-13837 NN denotes role
T6986 13838-13840 IN denotes in
T6987 13841-13847 NN denotes tissue
T6988 13848-13858 NN denotes remodeling
T6989 13859-13865 IN denotes during
T6990 13866-13877 NN denotes development
T6991 13877-13878 . denotes .
T6992 13878-14091 sentence denotes Starting at E12.5, we analyzed and compared the number and distribution of aCasp3-positive cells in over 140 serial sections of three wild-type and six Ptdsr -/- embryos in consecutive and corresponding sections.
T6993 13879-13887 VBG denotes Starting
T6995 13888-13890 IN denotes at
T6996 13891-13896 NN denotes E12.5
T6997 13896-13898 , denotes ,
T6998 13898-13900 PRP denotes we
T6994 13901-13909 VBD denotes analyzed
T6999 13910-13913 CC denotes and
T7000 13914-13922 VBD denotes compared
T7001 13923-13926 DT denotes the
T7002 13927-13933 NN denotes number
T7003 13934-13937 CC denotes and
T7004 13938-13950 NN denotes distribution
T7005 13951-13953 IN denotes of
T7006 13954-13960 NN denotes aCasp3
T7008 13960-13961 HYPH denotes -
T7007 13961-13969 JJ denotes positive
T7009 13970-13975 NNS denotes cells
T7010 13976-13978 IN denotes in
T7011 13979-13983 IN denotes over
T7012 13984-13987 CD denotes 140
T7014 13988-13994 JJ denotes serial
T7013 13995-14003 NNS denotes sections
T7015 14004-14006 IN denotes of
T7016 14007-14012 CD denotes three
T7018 14013-14017 JJ denotes wild
T7019 14017-14018 HYPH denotes -
T7017 14018-14022 NN denotes type
T7020 14023-14026 CC denotes and
T7021 14027-14030 CD denotes six
T7022 14031-14036 NN denotes Ptdsr
T7024 14037-14038 SYM denotes -
T7025 14038-14039 HYPH denotes /
T7026 14039-14040 SYM denotes -
T7023 14041-14048 NNS denotes embryos
T7027 14049-14051 IN denotes in
T7028 14052-14063 JJ denotes consecutive
T7030 14064-14067 CC denotes and
T7031 14068-14081 JJ denotes corresponding
T7029 14082-14090 NNS denotes sections
T7032 14090-14091 . denotes .
T7033 14091-14210 sentence denotes The sagittal sections were separated by 5 μm, allowing a detailed analysis of apoptosis in several organs and tissues.
T7034 14092-14095 DT denotes The
T7036 14096-14104 JJ denotes sagittal
T7035 14105-14113 NNS denotes sections
T7038 14114-14118 VBD denotes were
T7037 14119-14128 VBN denotes separated
T7039 14129-14131 IN denotes by
T7040 14132-14133 CD denotes 5
T7041 14134-14136 NNS denotes μm
T7042 14136-14138 , denotes ,
T7043 14138-14146 VBG denotes allowing
T7044 14147-14148 DT denotes a
T7046 14149-14157 JJ denotes detailed
T7045 14158-14166 NN denotes analysis
T7047 14167-14169 IN denotes of
T7048 14170-14179 NN denotes apoptosis
T7049 14180-14182 IN denotes in
T7050 14183-14190 JJ denotes several
T7051 14191-14197 NNS denotes organs
T7052 14198-14201 CC denotes and
T7053 14202-14209 NNS denotes tissues
T7054 14209-14210 . denotes .
T7055 14210-14434 sentence denotes Tissue restructuring by programmed cell death occurred most notably within the ventral part of the neural tube (Figure 6b,f) and in the developing paravertebral ganglia (Figure 6d,h) with many apoptotic cells being present.
T7056 14211-14217 NN denotes Tissue
T7057 14218-14231 NN denotes restructuring
T7059 14232-14234 IN denotes by
T7060 14235-14245 VBN denotes programmed
T7062 14246-14250 NN denotes cell
T7061 14251-14256 NN denotes death
T7058 14257-14265 VBD denotes occurred
T7063 14266-14270 RBS denotes most
T7064 14271-14278 RB denotes notably
T7065 14279-14285 IN denotes within
T7066 14286-14289 DT denotes the
T7068 14290-14297 JJ denotes ventral
T7067 14298-14302 NN denotes part
T7069 14303-14305 IN denotes of
T7070 14306-14309 DT denotes the
T7072 14310-14316 JJ denotes neural
T7071 14317-14321 NN denotes tube
T7073 14322-14323 -LRB- denotes (
T7075 14323-14329 NN denotes Figure
T7076 14330-14332 NN denotes 6b
T7077 14332-14333 , denotes ,
T7074 14333-14334 NN denotes f
T7078 14334-14335 -RRB- denotes )
T7079 14336-14339 CC denotes and
T7080 14340-14342 IN denotes in
T7081 14343-14346 DT denotes the
T7083 14347-14357 VBG denotes developing
T7084 14358-14371 JJ denotes paravertebral
T7082 14372-14379 NNS denotes ganglia
T7085 14380-14381 -LRB- denotes (
T7087 14381-14387 NN denotes Figure
T7088 14388-14390 NN denotes 6d
T7089 14390-14391 , denotes ,
T7086 14391-14392 NN denotes h
T7090 14392-14393 -RRB- denotes )
T7091 14394-14398 IN denotes with
T7092 14399-14403 JJ denotes many
T7094 14404-14413 JJ denotes apoptotic
T7093 14414-14419 NNS denotes cells
T7095 14420-14425 VBG denotes being
T7096 14426-14433 JJ denotes present
T7097 14433-14434 . denotes .
T7098 14434-14612 sentence denotes In these tissues Ptdsr is highly expressed at E12.5 (Figure 2c) but we observed no difference in the number or distribution of apoptotic cells in Ptdsr+/+ and Ptdsr -/- embryos.
T7099 14435-14437 IN denotes In
T7101 14438-14443 DT denotes these
T7102 14444-14451 NNS denotes tissues
T7103 14452-14457 NN denotes Ptdsr
T7100 14458-14460 VBZ denotes is
T7104 14461-14467 RB denotes highly
T7105 14468-14477 VBN denotes expressed
T7106 14478-14480 IN denotes at
T7107 14481-14486 NN denotes E12.5
T7108 14487-14488 -LRB- denotes (
T7110 14488-14494 NN denotes Figure
T7109 14495-14497 NN denotes 2c
T7111 14497-14498 -RRB- denotes )
T7112 14499-14502 CC denotes but
T7113 14503-14505 PRP denotes we
T7114 14506-14514 VBD denotes observed
T7115 14515-14517 DT denotes no
T7116 14518-14528 NN denotes difference
T7117 14529-14531 IN denotes in
T7118 14532-14535 DT denotes the
T7119 14536-14542 NN denotes number
T7120 14543-14545 CC denotes or
T7121 14546-14558 NN denotes distribution
T7122 14559-14561 IN denotes of
T7123 14562-14571 JJ denotes apoptotic
T7124 14572-14577 NNS denotes cells
T7125 14578-14580 IN denotes in
T7126 14581-14586 NN denotes Ptdsr
T7128 14586-14587 SYM denotes +
T7129 14587-14588 HYPH denotes /
T7130 14588-14589 SYM denotes +
T7131 14590-14593 CC denotes and
T7132 14594-14599 NN denotes Ptdsr
T7133 14600-14601 SYM denotes -
T7134 14601-14602 HYPH denotes /
T7135 14602-14603 SYM denotes -
T7127 14604-14611 NNS denotes embryos
T7136 14611-14612 . denotes .
T7137 14612-14852 sentence denotes The same was true for the developing kidney: apoptotic cells were present in Ptdsr+/+ and Ptdsr -/- embryos, in limited numbers, but we failed to detect any differences in the number of apoptotic cells between the genotypes (Figure 6c,6g).
T7138 14613-14616 DT denotes The
T7139 14617-14621 JJ denotes same
T7140 14622-14625 VBD denotes was
T7142 14626-14630 JJ denotes true
T7143 14631-14634 IN denotes for
T7144 14635-14638 DT denotes the
T7146 14639-14649 VBG denotes developing
T7145 14650-14656 NN denotes kidney
T7147 14656-14658 : denotes :
T7148 14658-14667 JJ denotes apoptotic
T7149 14668-14673 NNS denotes cells
T7141 14674-14678 VBD denotes were
T7150 14679-14686 JJ denotes present
T7151 14687-14689 IN denotes in
T7152 14690-14695 NN denotes Ptdsr
T7154 14695-14696 SYM denotes +
T7155 14696-14697 HYPH denotes /
T7156 14697-14698 SYM denotes +
T7157 14699-14702 CC denotes and
T7158 14703-14708 NN denotes Ptdsr
T7159 14709-14710 SYM denotes -
T7160 14710-14711 HYPH denotes /
T7161 14711-14712 SYM denotes -
T7153 14713-14720 NNS denotes embryos
T7162 14720-14722 , denotes ,
T7163 14722-14724 IN denotes in
T7164 14725-14732 JJ denotes limited
T7165 14733-14740 NNS denotes numbers
T7166 14740-14742 , denotes ,
T7167 14742-14745 CC denotes but
T7168 14746-14748 PRP denotes we
T7169 14749-14755 VBD denotes failed
T7170 14756-14758 TO denotes to
T7171 14759-14765 VB denotes detect
T7172 14766-14769 DT denotes any
T7173 14770-14781 NNS denotes differences
T7174 14782-14784 IN denotes in
T7175 14785-14788 DT denotes the
T7176 14789-14795 NN denotes number
T7177 14796-14798 IN denotes of
T7178 14799-14808 JJ denotes apoptotic
T7179 14809-14814 NNS denotes cells
T7180 14815-14822 IN denotes between
T7181 14823-14826 DT denotes the
T7182 14827-14836 NNS denotes genotypes
T7183 14837-14838 -LRB- denotes (
T7185 14838-14844 NN denotes Figure
T7186 14845-14847 NN denotes 6c
T7187 14847-14848 , denotes ,
T7184 14848-14850 NN denotes 6g
T7188 14850-14851 -RRB- denotes )
T7189 14851-14852 . denotes .
T7190 14852-15090 sentence denotes Furthermore, when we continued our analysis of apoptotic cell clearance in vivo at E16.5, E17.5 and E18.5 of embryonic development as well as in neonatal mice, the number and distribution of apoptotic cells was similar in both genotypes.
T7191 14853-14864 RB denotes Furthermore
T7193 14864-14866 , denotes ,
T7194 14866-14870 WRB denotes when
T7196 14871-14873 PRP denotes we
T7195 14874-14883 VBD denotes continued
T7197 14884-14887 PRP$ denotes our
T7198 14888-14896 NN denotes analysis
T7199 14897-14899 IN denotes of
T7200 14900-14909 JJ denotes apoptotic
T7201 14910-14914 NN denotes cell
T7202 14915-14924 NN denotes clearance
T7203 14925-14927 FW denotes in
T7204 14928-14932 FW denotes vivo
T7205 14933-14935 IN denotes at
T7206 14936-14941 NN denotes E16.5
T7207 14941-14943 , denotes ,
T7208 14943-14948 NN denotes E17.5
T7209 14949-14952 CC denotes and
T7210 14953-14958 NN denotes E18.5
T7211 14959-14961 IN denotes of
T7212 14962-14971 JJ denotes embryonic
T7213 14972-14983 NN denotes development
T7214 14984-14986 RB denotes as
T7216 14987-14991 RB denotes well
T7215 14992-14994 IN denotes as
T7217 14995-14997 IN denotes in
T7218 14998-15006 JJ denotes neonatal
T7219 15007-15011 NNS denotes mice
T7220 15011-15013 , denotes ,
T7221 15013-15016 DT denotes the
T7222 15017-15023 NN denotes number
T7223 15024-15027 CC denotes and
T7224 15028-15040 NN denotes distribution
T7225 15041-15043 IN denotes of
T7226 15044-15053 JJ denotes apoptotic
T7227 15054-15059 NNS denotes cells
T7192 15060-15063 VBD denotes was
T7228 15064-15071 JJ denotes similar
T7229 15072-15074 IN denotes in
T7230 15075-15079 DT denotes both
T7231 15080-15089 NNS denotes genotypes
T7232 15089-15090 . denotes .
T7233 15090-15325 sentence denotes As already observed at E12.5, analysis of aCasp3-stained sections of the developing thymus, heart, diaphragm, genital ridge, eyes and retina convincingly showed that there was no impairment in apoptotic cell removal in Ptdsr -/- mice.
T7234 15091-15093 IN denotes As
T7236 15094-15101 RB denotes already
T7235 15102-15110 VBN denotes observed
T7238 15111-15113 IN denotes at
T7239 15114-15119 NN denotes E12.5
T7240 15119-15121 , denotes ,
T7241 15121-15129 NN denotes analysis
T7242 15130-15132 IN denotes of
T7243 15133-15139 NN denotes aCasp3
T7245 15139-15140 HYPH denotes -
T7244 15140-15147 VBN denotes stained
T7246 15148-15156 NNS denotes sections
T7247 15157-15159 IN denotes of
T7248 15160-15163 DT denotes the
T7250 15164-15174 VBG denotes developing
T7249 15175-15181 NN denotes thymus
T7251 15181-15183 , denotes ,
T7252 15183-15188 NN denotes heart
T7253 15188-15190 , denotes ,
T7254 15190-15199 NN denotes diaphragm
T7255 15199-15201 , denotes ,
T7256 15201-15208 JJ denotes genital
T7257 15209-15214 NN denotes ridge
T7258 15214-15216 , denotes ,
T7259 15216-15220 NNS denotes eyes
T7260 15221-15224 CC denotes and
T7261 15225-15231 NN denotes retina
T7262 15232-15244 RB denotes convincingly
T7237 15245-15251 VBD denotes showed
T7263 15252-15256 IN denotes that
T7265 15257-15262 EX denotes there
T7264 15263-15266 VBD denotes was
T7266 15267-15269 DT denotes no
T7267 15270-15280 NN denotes impairment
T7268 15281-15283 IN denotes in
T7269 15284-15293 JJ denotes apoptotic
T7271 15294-15298 NN denotes cell
T7270 15299-15306 NN denotes removal
T7272 15307-15309 IN denotes in
T7273 15310-15315 NN denotes Ptdsr
T7275 15316-15317 SYM denotes -
T7276 15317-15318 HYPH denotes /
T7277 15318-15319 SYM denotes -
T7274 15320-15324 NNS denotes mice
T7278 15324-15325 . denotes .
T7279 15325-15560 sentence denotes Moreover, because Li and colleagues [31] reported impaired clearance of dead cells during lung development in Ptdsr-deficient mice, we examined the rate of apoptosis induction and cell clearance in our Ptdsr-knockout mice in the lung.
T7280 15326-15334 RB denotes Moreover
T7282 15334-15336 , denotes ,
T7283 15336-15343 IN denotes because
T7285 15344-15346 NNP denotes Li
T7286 15347-15350 CC denotes and
T7287 15351-15361 NNS denotes colleagues
T7288 15362-15363 -LRB- denotes [
T7289 15363-15365 CD denotes 31
T7290 15365-15366 -RRB- denotes ]
T7284 15367-15375 VBD denotes reported
T7291 15376-15384 JJ denotes impaired
T7292 15385-15394 NN denotes clearance
T7293 15395-15397 IN denotes of
T7294 15398-15402 JJ denotes dead
T7295 15403-15408 NNS denotes cells
T7296 15409-15415 IN denotes during
T7297 15416-15420 NN denotes lung
T7298 15421-15432 NN denotes development
T7299 15433-15435 IN denotes in
T7300 15436-15441 NN denotes Ptdsr
T7302 15441-15442 HYPH denotes -
T7301 15442-15451 JJ denotes deficient
T7303 15452-15456 NNS denotes mice
T7304 15456-15458 , denotes ,
T7305 15458-15460 PRP denotes we
T7281 15461-15469 VBD denotes examined
T7306 15470-15473 DT denotes the
T7307 15474-15478 NN denotes rate
T7308 15479-15481 IN denotes of
T7309 15482-15491 NN denotes apoptosis
T7310 15492-15501 NN denotes induction
T7311 15502-15505 CC denotes and
T7312 15506-15510 NN denotes cell
T7313 15511-15520 NN denotes clearance
T7314 15521-15523 IN denotes in
T7315 15524-15527 PRP$ denotes our
T7317 15528-15533 NN denotes Ptdsr
T7319 15533-15534 HYPH denotes -
T7318 15534-15542 NN denotes knockout
T7316 15543-15547 NNS denotes mice
T7320 15548-15550 IN denotes in
T7321 15551-15554 DT denotes the
T7322 15555-15559 NN denotes lung
T7323 15559-15560 . denotes .
T7324 15560-15745 sentence denotes Analysis of aCasp3-stained lung tissue from Ptdsr+/+ and Ptdsr -/- mice at E17.5 and P0 demonstrated that apoptosis was an extremely rare event during lung morphogenesis at this stage.
T7325 15561-15569 NN denotes Analysis
T7327 15570-15572 IN denotes of
T7328 15573-15579 NN denotes aCasp3
T7330 15579-15580 HYPH denotes -
T7329 15580-15587 VBN denotes stained
T7332 15588-15592 NN denotes lung
T7331 15593-15599 NN denotes tissue
T7333 15600-15604 IN denotes from
T7334 15605-15610 NN denotes Ptdsr
T7336 15610-15611 SYM denotes +
T7337 15611-15612 HYPH denotes /
T7338 15612-15613 SYM denotes +
T7339 15614-15617 CC denotes and
T7340 15618-15623 NN denotes Ptdsr
T7341 15624-15625 SYM denotes -
T7342 15625-15626 HYPH denotes /
T7343 15626-15627 SYM denotes -
T7335 15628-15632 NNS denotes mice
T7344 15633-15635 IN denotes at
T7345 15636-15641 NN denotes E17.5
T7346 15642-15645 CC denotes and
T7347 15646-15648 NN denotes P0
T7326 15649-15661 VBD denotes demonstrated
T7348 15662-15666 IN denotes that
T7350 15667-15676 NN denotes apoptosis
T7349 15677-15680 VBD denotes was
T7351 15681-15683 DT denotes an
T7353 15684-15693 RB denotes extremely
T7354 15694-15698 JJ denotes rare
T7352 15699-15704 NN denotes event
T7355 15705-15711 IN denotes during
T7356 15712-15716 NN denotes lung
T7357 15717-15730 NN denotes morphogenesis
T7358 15731-15733 IN denotes at
T7359 15734-15738 DT denotes this
T7360 15739-15744 NN denotes stage
T7361 15744-15745 . denotes .
T7362 15745-15866 sentence denotes In addition, there were no differences in the number or distribution of apoptotic cells in Ptdsr -/- and Ptdsr +/+ mice.
T7363 15746-15748 IN denotes In
T7365 15749-15757 NN denotes addition
T7366 15757-15759 , denotes ,
T7367 15759-15764 EX denotes there
T7364 15765-15769 VBD denotes were
T7368 15770-15772 DT denotes no
T7369 15773-15784 NNS denotes differences
T7370 15785-15787 IN denotes in
T7371 15788-15791 DT denotes the
T7372 15792-15798 NN denotes number
T7373 15799-15801 CC denotes or
T7374 15802-15814 NN denotes distribution
T7375 15815-15817 IN denotes of
T7376 15818-15827 JJ denotes apoptotic
T7377 15828-15833 NNS denotes cells
T7378 15834-15836 IN denotes in
T7379 15837-15842 NN denotes Ptdsr
T7381 15843-15844 SYM denotes -
T7382 15844-15845 HYPH denotes /
T7383 15845-15846 SYM denotes -
T7384 15847-15850 CC denotes and
T7385 15851-15856 NN denotes Ptdsr
T7386 15857-15858 SYM denotes +
T7387 15858-15859 HYPH denotes /
T7388 15859-15860 SYM denotes +
T7380 15861-15865 NNS denotes mice
T7389 15865-15866 . denotes .
T7390 15866-15972 sentence denotes Furthermore, we were unable to detect any evidence of tissue necrosis in lungs from Ptdsr-deficient mice.
T7391 15867-15878 RB denotes Furthermore
T7393 15878-15880 , denotes ,
T7394 15880-15882 PRP denotes we
T7392 15883-15887 VBD denotes were
T7395 15888-15894 JJ denotes unable
T7396 15895-15897 TO denotes to
T7397 15898-15904 VB denotes detect
T7398 15905-15908 DT denotes any
T7399 15909-15917 NN denotes evidence
T7400 15918-15920 IN denotes of
T7401 15921-15927 NN denotes tissue
T7402 15928-15936 NN denotes necrosis
T7403 15937-15939 IN denotes in
T7404 15940-15945 NNS denotes lungs
T7405 15946-15950 IN denotes from
T7406 15951-15956 NN denotes Ptdsr
T7408 15956-15957 HYPH denotes -
T7407 15957-15966 JJ denotes deficient
T7409 15967-15971 NNS denotes mice
T7410 15971-15972 . denotes .
T7411 15972-16160 sentence denotes In contrast to the report of Li et al. [31], we never observed recruitment of neutrophils or other signs of pulmonary inflammation at any stage of development in our Ptdsr-deficient mice.
T7412 15973-15975 IN denotes In
T7414 15976-15984 NN denotes contrast
T7415 15985-15987 IN denotes to
T7416 15988-15991 DT denotes the
T7417 15992-15998 NN denotes report
T7418 15999-16001 IN denotes of
T7419 16002-16004 NNP denotes Li
T7420 16005-16007 FW denotes et
T7421 16008-16011 FW denotes al.
T7422 16012-16013 -LRB- denotes [
T7423 16013-16015 CD denotes 31
T7424 16015-16016 -RRB- denotes ]
T7425 16016-16018 , denotes ,
T7426 16018-16020 PRP denotes we
T7427 16021-16026 RB denotes never
T7413 16027-16035 VBD denotes observed
T7428 16036-16047 NN denotes recruitment
T7429 16048-16050 IN denotes of
T7430 16051-16062 NNS denotes neutrophils
T7431 16063-16065 CC denotes or
T7432 16066-16071 JJ denotes other
T7433 16072-16077 NNS denotes signs
T7434 16078-16080 IN denotes of
T7435 16081-16090 JJ denotes pulmonary
T7436 16091-16103 NN denotes inflammation
T7437 16104-16106 IN denotes at
T7438 16107-16110 DT denotes any
T7439 16111-16116 NN denotes stage
T7440 16117-16119 IN denotes of
T7441 16120-16131 NN denotes development
T7442 16132-16134 IN denotes in
T7443 16135-16138 PRP$ denotes our
T7445 16139-16144 NN denotes Ptdsr
T7447 16144-16145 HYPH denotes -
T7446 16145-16154 JJ denotes deficient
T7444 16155-16159 NNS denotes mice
T7448 16159-16160 . denotes .
T7449 16160-16370 sentence denotes To analyze whether macrophages are recruited into areas where apoptosis is prominent during embryogenesis, we stained consecutive serial sections either with the macrophage surface marker F4/80 or with aCasp3.
T7450 16161-16163 TO denotes To
T7451 16164-16171 VB denotes analyze
T7453 16172-16179 IN denotes whether
T7455 16180-16191 NNS denotes macrophages
T7456 16192-16195 VBP denotes are
T7454 16196-16205 VBN denotes recruited
T7457 16206-16210 IN denotes into
T7458 16211-16216 NNS denotes areas
T7459 16217-16222 WRB denotes where
T7461 16223-16232 NN denotes apoptosis
T7460 16233-16235 VBZ denotes is
T7462 16236-16245 JJ denotes prominent
T7463 16246-16252 IN denotes during
T7464 16253-16266 NN denotes embryogenesis
T7465 16266-16268 , denotes ,
T7466 16268-16270 PRP denotes we
T7452 16271-16278 VBD denotes stained
T7467 16279-16290 JJ denotes consecutive
T7469 16291-16297 JJ denotes serial
T7468 16298-16306 NNS denotes sections
T7470 16307-16313 CC denotes either
T7471 16314-16318 IN denotes with
T7472 16319-16322 DT denotes the
T7474 16323-16333 NN denotes macrophage
T7475 16334-16341 NN denotes surface
T7473 16342-16348 NN denotes marker
T7476 16349-16351 NN denotes F4
T7477 16351-16352 HYPH denotes /
T7478 16352-16354 CD denotes 80
T7479 16355-16357 CC denotes or
T7480 16358-16362 IN denotes with
T7481 16363-16369 NN denotes aCasp3
T7482 16369-16370 . denotes .
T7483 16370-16450 sentence denotes Surprisingly, there was no co-localization of macrophages with apoptotic cells.
T7484 16371-16383 RB denotes Surprisingly
T7486 16383-16385 , denotes ,
T7487 16385-16390 EX denotes there
T7485 16391-16394 VBD denotes was
T7488 16395-16397 DT denotes no
T7489 16398-16413 NN denotes co-localization
T7490 16414-16416 IN denotes of
T7491 16417-16428 NNS denotes macrophages
T7492 16429-16433 IN denotes with
T7493 16434-16443 JJ denotes apoptotic
T7494 16444-16449 NNS denotes cells
T7495 16449-16450 . denotes .
T7496 16450-16623 sentence denotes In virtually all embryonic tissues, apoptotic cells and macrophages were localized in different compartments (Figure 6e,6i; and see also Additional data file 1, Figure S1).
T7497 16451-16453 IN denotes In
T7499 16454-16463 RB denotes virtually
T7501 16464-16467 DT denotes all
T7502 16468-16477 JJ denotes embryonic
T7500 16478-16485 NNS denotes tissues
T7503 16485-16487 , denotes ,
T7504 16487-16496 JJ denotes apoptotic
T7505 16497-16502 NNS denotes cells
T7506 16503-16506 CC denotes and
T7507 16507-16518 NNS denotes macrophages
T7508 16519-16523 VBD denotes were
T7498 16524-16533 VBN denotes localized
T7509 16534-16536 IN denotes in
T7510 16537-16546 JJ denotes different
T7511 16547-16559 NNS denotes compartments
T7512 16560-16561 -LRB- denotes (
T7514 16561-16567 NN denotes Figure
T7515 16568-16570 NN denotes 6e
T7516 16570-16571 , denotes ,
T7513 16571-16573 NN denotes 6i
T7517 16573-16574 : denotes ;
T7518 16575-16578 CC denotes and
T7519 16579-16582 VB denotes see
T7520 16583-16587 RB denotes also
T7521 16588-16598 JJ denotes Additional
T7522 16599-16603 NNS denotes data
T7523 16604-16608 NN denotes file
T7524 16609-16610 CD denotes 1
T7525 16610-16612 , denotes ,
T7526 16612-16618 NN denotes Figure
T7527 16619-16621 NN denotes S1
T7528 16621-16622 -RRB- denotes )
T7529 16622-16623 . denotes .
T7530 16623-16789 sentence denotes This suggests that at this stage of development it is mainly neighboring cells that are involved in removal of apoptotic cells, rather than professional macrophages.
T7531 16624-16628 DT denotes This
T7532 16629-16637 VBZ denotes suggests
T7533 16638-16642 IN denotes that
T7535 16643-16645 IN denotes at
T7536 16646-16650 DT denotes this
T7537 16651-16656 NN denotes stage
T7538 16657-16659 IN denotes of
T7539 16660-16671 NN denotes development
T7540 16672-16674 PRP denotes it
T7534 16675-16677 VBZ denotes is
T7541 16678-16684 RB denotes mainly
T7542 16685-16696 VBG denotes neighboring
T7543 16697-16702 NNS denotes cells
T7544 16703-16707 WDT denotes that
T7546 16708-16711 VBP denotes are
T7545 16712-16720 VBN denotes involved
T7547 16721-16723 IN denotes in
T7548 16724-16731 NN denotes removal
T7549 16732-16734 IN denotes of
T7550 16735-16744 JJ denotes apoptotic
T7551 16745-16750 NNS denotes cells
T7552 16750-16752 , denotes ,
T7553 16752-16758 RB denotes rather
T7554 16759-16763 IN denotes than
T7555 16764-16776 JJ denotes professional
T7556 16777-16788 NNS denotes macrophages
T7557 16788-16789 . denotes .
T7558 16789-17042 sentence denotes In summary, our analysis in vivo did not reveal any impairment in apoptotic cell clearance in Ptdsr-deficient embryos during development and further suggests that phagocytosis of apoptotic cells is mainly mediated by non-professional 'bystander' cells.
T7559 16790-16792 IN denotes In
T7561 16793-16800 NN denotes summary
T7562 16800-16802 , denotes ,
T7563 16802-16805 PRP$ denotes our
T7564 16806-16814 NN denotes analysis
T7565 16815-16817 FW denotes in
T7566 16818-16822 FW denotes vivo
T7567 16823-16826 VBD denotes did
T7568 16827-16830 RB denotes not
T7560 16831-16837 VB denotes reveal
T7569 16838-16841 DT denotes any
T7570 16842-16852 NN denotes impairment
T7571 16853-16855 IN denotes in
T7572 16856-16865 JJ denotes apoptotic
T7574 16866-16870 NN denotes cell
T7573 16871-16880 NN denotes clearance
T7575 16881-16883 IN denotes in
T7576 16884-16889 NN denotes Ptdsr
T7578 16889-16890 HYPH denotes -
T7577 16890-16899 JJ denotes deficient
T7579 16900-16907 NNS denotes embryos
T7580 16908-16914 IN denotes during
T7581 16915-16926 NN denotes development
T7582 16927-16930 CC denotes and
T7583 16931-16938 RBR denotes further
T7584 16939-16947 VBZ denotes suggests
T7585 16948-16952 IN denotes that
T7587 16953-16965 NN denotes phagocytosis
T7588 16966-16968 IN denotes of
T7589 16969-16978 JJ denotes apoptotic
T7590 16979-16984 NNS denotes cells
T7591 16985-16987 VBZ denotes is
T7592 16988-16994 RB denotes mainly
T7586 16995-17003 VBN denotes mediated
T7593 17004-17006 IN denotes by
T7594 17007-17023 JJ denotes non-professional
T7596 17024-17025 `` denotes '
T7597 17025-17034 NN denotes bystander
T7598 17034-17035 '' denotes '
T7595 17036-17041 NNS denotes cells
T7599 17041-17042 . denotes .
T7600 17042-17284 sentence denotes To determine whether macrophages from Ptdsr-knockout mice were impaired in the efficacy of apoptotic cell uptake in vitro, we performed phagocytosis assays with fetal-liver-derived macrophages (FLDMs) and quantified their phagocytosis rates.
T7601 17043-17045 TO denotes To
T7602 17046-17055 VB denotes determine
T7604 17056-17063 IN denotes whether
T7606 17064-17075 NNS denotes macrophages
T7607 17076-17080 IN denotes from
T7608 17081-17086 NN denotes Ptdsr
T7610 17086-17087 HYPH denotes -
T7609 17087-17095 NN denotes knockout
T7611 17096-17100 NNS denotes mice
T7612 17101-17105 VBD denotes were
T7605 17106-17114 VBN denotes impaired
T7613 17115-17117 IN denotes in
T7614 17118-17121 DT denotes the
T7615 17122-17130 NN denotes efficacy
T7616 17131-17133 IN denotes of
T7617 17134-17143 JJ denotes apoptotic
T7618 17144-17148 NN denotes cell
T7619 17149-17155 NN denotes uptake
T7620 17156-17158 FW denotes in
T7621 17159-17164 FW denotes vitro
T7622 17164-17166 , denotes ,
T7623 17166-17168 PRP denotes we
T7603 17169-17178 VBD denotes performed
T7624 17179-17191 NN denotes phagocytosis
T7625 17192-17198 NNS denotes assays
T7626 17199-17203 IN denotes with
T7627 17204-17209 JJ denotes fetal
T7629 17209-17210 HYPH denotes -
T7628 17210-17215 NN denotes liver
T7631 17215-17216 HYPH denotes -
T7630 17216-17223 VBN denotes derived
T7632 17224-17235 NNS denotes macrophages
T7633 17236-17237 -LRB- denotes (
T7634 17237-17242 NNS denotes FLDMs
T7635 17242-17243 -RRB- denotes )
T7636 17244-17247 CC denotes and
T7637 17248-17258 VBD denotes quantified
T7638 17259-17264 PRP$ denotes their
T7640 17265-17277 NN denotes phagocytosis
T7639 17278-17283 NNS denotes rates
T7641 17283-17284 . denotes .
T7642 17284-17420 sentence denotes Phagocytosis of apoptotic thymocytes was investigated at 60, 90 and 120 minutes after addition of target cells in the absence of serum.
T7643 17285-17297 NN denotes Phagocytosis
T7645 17298-17300 IN denotes of
T7646 17301-17310 JJ denotes apoptotic
T7647 17311-17321 NNS denotes thymocytes
T7648 17322-17325 VBD denotes was
T7644 17326-17338 VBN denotes investigated
T7649 17339-17341 IN denotes at
T7650 17342-17344 CD denotes 60
T7652 17344-17346 , denotes ,
T7653 17346-17348 CD denotes 90
T7654 17349-17352 CC denotes and
T7655 17353-17356 CD denotes 120
T7651 17357-17364 NNS denotes minutes
T7656 17365-17370 IN denotes after
T7657 17371-17379 NN denotes addition
T7658 17380-17382 IN denotes of
T7659 17383-17389 NN denotes target
T7660 17390-17395 NNS denotes cells
T7661 17396-17398 IN denotes in
T7662 17399-17402 DT denotes the
T7663 17403-17410 NN denotes absence
T7664 17411-17413 IN denotes of
T7665 17414-17419 NN denotes serum
T7666 17419-17420 . denotes .
T7667 17420-17702 sentence denotes Analysis of phagocytosis rates by flow cytometric analysis (FACS) revealed no differences in the efficacy of apoptotic cell uptake between Ptdsr -/- and Ptdsr+/+ macrophages and demonstrated no differences in apoptotic cell engulfment between selected time points (data not shown).
T7668 17421-17429 NN denotes Analysis
T7670 17430-17432 IN denotes of
T7671 17433-17445 NN denotes phagocytosis
T7672 17446-17451 NNS denotes rates
T7673 17452-17454 IN denotes by
T7674 17455-17459 NN denotes flow
T7676 17460-17470 JJ denotes cytometric
T7675 17471-17479 NN denotes analysis
T7677 17480-17481 -LRB- denotes (
T7678 17481-17485 NN denotes FACS
T7679 17485-17486 -RRB- denotes )
T7669 17487-17495 VBD denotes revealed
T7680 17496-17498 DT denotes no
T7681 17499-17510 NNS denotes differences
T7682 17511-17513 IN denotes in
T7683 17514-17517 DT denotes the
T7684 17518-17526 NN denotes efficacy
T7685 17527-17529 IN denotes of
T7686 17530-17539 JJ denotes apoptotic
T7688 17540-17544 NN denotes cell
T7687 17545-17551 NN denotes uptake
T7689 17552-17559 IN denotes between
T7690 17560-17565 NN denotes Ptdsr
T7692 17566-17567 SYM denotes -
T7693 17567-17568 HYPH denotes /
T7694 17568-17569 SYM denotes -
T7695 17570-17573 CC denotes and
T7696 17574-17579 NN denotes Ptdsr
T7697 17579-17580 SYM denotes +
T7698 17580-17581 HYPH denotes /
T7699 17581-17582 SYM denotes +
T7691 17583-17594 NNS denotes macrophages
T7700 17595-17598 CC denotes and
T7701 17599-17611 VBD denotes demonstrated
T7702 17612-17614 DT denotes no
T7703 17615-17626 NNS denotes differences
T7704 17627-17629 IN denotes in
T7705 17630-17639 JJ denotes apoptotic
T7707 17640-17644 NN denotes cell
T7706 17645-17655 NN denotes engulfment
T7708 17656-17663 IN denotes between
T7709 17664-17672 VBN denotes selected
T7711 17673-17677 NN denotes time
T7710 17678-17684 NNS denotes points
T7712 17685-17686 -LRB- denotes (
T7714 17686-17690 NNS denotes data
T7715 17691-17694 RB denotes not
T7713 17695-17700 VBN denotes shown
T7716 17700-17701 -RRB- denotes )
T7717 17701-17702 . denotes .
T7718 17702-18019 sentence denotes To re-examine and further independently validate the result of normal apoptotic cell uptake by Ptdsr -/- macrophages, we performed phagocytosis assays for 60 min and determined the percentage of macrophages that had engulfed apoptotic cells, in a total of at least 300 macrophages counted by fluorescence microscopy.
T7719 17703-17705 TO denotes To
T7720 17706-17716 VB denotes re-examine
T7722 17717-17720 CC denotes and
T7723 17721-17728 RBR denotes further
T7725 17729-17742 RB denotes independently
T7724 17743-17751 VB denotes validate
T7726 17752-17755 DT denotes the
T7727 17756-17762 NN denotes result
T7728 17763-17765 IN denotes of
T7729 17766-17772 JJ denotes normal
T7731 17773-17782 JJ denotes apoptotic
T7732 17783-17787 NN denotes cell
T7730 17788-17794 NN denotes uptake
T7733 17795-17797 IN denotes by
T7734 17798-17803 NN denotes Ptdsr
T7736 17804-17805 SYM denotes -
T7737 17805-17806 HYPH denotes /
T7738 17806-17807 SYM denotes -
T7735 17808-17819 NNS denotes macrophages
T7739 17819-17821 , denotes ,
T7740 17821-17823 PRP denotes we
T7721 17824-17833 VBD denotes performed
T7741 17834-17846 NN denotes phagocytosis
T7742 17847-17853 NNS denotes assays
T7743 17854-17857 IN denotes for
T7744 17858-17860 CD denotes 60
T7745 17861-17864 NNS denotes min
T7746 17865-17868 CC denotes and
T7747 17869-17879 VBD denotes determined
T7748 17880-17883 DT denotes the
T7749 17884-17894 NN denotes percentage
T7750 17895-17897 IN denotes of
T7751 17898-17909 NNS denotes macrophages
T7752 17910-17914 WDT denotes that
T7754 17915-17918 VBD denotes had
T7753 17919-17927 VBN denotes engulfed
T7755 17928-17937 JJ denotes apoptotic
T7756 17938-17943 NNS denotes cells
T7757 17943-17945 , denotes ,
T7758 17945-17947 IN denotes in
T7759 17948-17949 DT denotes a
T7760 17950-17955 NN denotes total
T7761 17956-17958 IN denotes of
T7762 17959-17961 RB denotes at
T7764 17962-17967 RBS denotes least
T7763 17968-17971 CD denotes 300
T7765 17972-17983 NNS denotes macrophages
T7766 17984-17991 VBN denotes counted
T7767 17992-17994 IN denotes by
T7768 17995-18007 NN denotes fluorescence
T7769 18008-18018 NN denotes microscopy
T7770 18018-18019 . denotes .
T7771 18019-18174 sentence denotes Phagocytosed, 5-carboxytetramethylrhodamine- (TAMRA-) labeled apoptotic cells were identified as being engulfed by inclusion in F4/80-labeled macrophages.
T7772 18020-18032 VBN denotes Phagocytosed
T7774 18032-18034 , denotes ,
T7775 18034-18035 CD denotes 5
T7777 18035-18036 HYPH denotes -
T7776 18036-18063 NN denotes carboxytetramethylrhodamine
T7779 18063-18064 HYPH denotes -
T7780 18065-18066 -LRB- denotes (
T7781 18066-18071 NN denotes TAMRA
T7782 18071-18072 HYPH denotes -
T7783 18072-18073 -RRB- denotes )
T7778 18074-18081 VBN denotes labeled
T7784 18082-18091 JJ denotes apoptotic
T7773 18092-18097 NNS denotes cells
T7786 18098-18102 VBD denotes were
T7785 18103-18113 VBN denotes identified
T7787 18114-18116 IN denotes as
T7789 18117-18122 VBG denotes being
T7788 18123-18131 VBN denotes engulfed
T7790 18132-18134 IN denotes by
T7791 18135-18144 NN denotes inclusion
T7792 18145-18147 IN denotes in
T7793 18148-18150 NN denotes F4
T7795 18150-18151 HYPH denotes /
T7796 18151-18153 CD denotes 80
T7797 18153-18154 HYPH denotes -
T7794 18154-18161 VBN denotes labeled
T7798 18162-18173 NNS denotes macrophages
T7799 18173-18174 . denotes .
T7800 18174-18297 sentence denotes Analysis was done independently by three investigators who were not aware of macrophage genotypes (Ptdsr -/- or Ptdsr+/+).
T7801 18175-18183 NN denotes Analysis
T7803 18184-18187 VBD denotes was
T7802 18188-18192 VBN denotes done
T7804 18193-18206 RB denotes independently
T7805 18207-18209 IN denotes by
T7806 18210-18215 CD denotes three
T7807 18216-18229 NNS denotes investigators
T7808 18230-18233 WP denotes who
T7809 18234-18238 VBD denotes were
T7810 18239-18242 RB denotes not
T7811 18243-18248 JJ denotes aware
T7812 18249-18251 IN denotes of
T7813 18252-18262 NN denotes macrophage
T7814 18263-18272 NNS denotes genotypes
T7815 18273-18274 -LRB- denotes (
T7816 18274-18279 NN denotes Ptdsr
T7817 18280-18281 SYM denotes -
T7818 18281-18282 HYPH denotes /
T7819 18282-18283 SYM denotes -
T7820 18284-18286 CC denotes or
T7821 18287-18292 NN denotes Ptdsr
T7822 18292-18293 SYM denotes +
T7823 18293-18294 HYPH denotes /
T7824 18294-18295 SYM denotes +
T7825 18295-18296 -RRB- denotes )
T7826 18296-18297 . denotes .
T7827 18297-18519 sentence denotes Again, no differences were found in the percentage of macrophages that had engulfed apoptotic cells (Figure 7a,c,e) or in the relative number of phagocytosed apoptotic cells per macrophage (phagocytotic index; Figure 7f).
T7828 18298-18303 RB denotes Again
T7830 18303-18305 , denotes ,
T7831 18305-18307 DT denotes no
T7832 18308-18319 NNS denotes differences
T7833 18320-18324 VBD denotes were
T7829 18325-18330 VBN denotes found
T7834 18331-18333 IN denotes in
T7835 18334-18337 DT denotes the
T7836 18338-18348 NN denotes percentage
T7837 18349-18351 IN denotes of
T7838 18352-18363 NNS denotes macrophages
T7839 18364-18368 WDT denotes that
T7841 18369-18372 VBD denotes had
T7840 18373-18381 VBN denotes engulfed
T7842 18382-18391 JJ denotes apoptotic
T7843 18392-18397 NNS denotes cells
T7844 18398-18399 -LRB- denotes (
T7846 18399-18405 NN denotes Figure
T7847 18406-18408 NN denotes 7a
T7848 18408-18409 , denotes ,
T7849 18409-18410 NN denotes c
T7850 18410-18411 , denotes ,
T7845 18411-18412 NN denotes e
T7851 18412-18413 -RRB- denotes )
T7852 18414-18416 CC denotes or
T7853 18417-18419 IN denotes in
T7854 18420-18423 DT denotes the
T7856 18424-18432 JJ denotes relative
T7855 18433-18439 NN denotes number
T7857 18440-18442 IN denotes of
T7858 18443-18455 VBN denotes phagocytosed
T7860 18456-18465 JJ denotes apoptotic
T7859 18466-18471 NNS denotes cells
T7861 18472-18475 IN denotes per
T7862 18476-18486 NN denotes macrophage
T7863 18487-18488 -LRB- denotes (
T7865 18488-18500 JJ denotes phagocytotic
T7866 18501-18506 NN denotes index
T7867 18506-18507 : denotes ;
T7868 18508-18514 NN denotes Figure
T7864 18515-18517 NN denotes 7f
T7869 18517-18518 -RRB- denotes )
T7870 18518-18519 . denotes .
T7871 18519-18674 sentence denotes Moreover, single Ptdsr -/-macrophages could be identified that had engulfed even more apoptotic target cells than had wild-type macrophages (Figure 7b,d).
T7872 18520-18528 RB denotes Moreover
T7874 18528-18530 , denotes ,
T7875 18530-18536 JJ denotes single
T7877 18537-18542 NN denotes Ptdsr
T7878 18543-18544 SYM denotes -
T7879 18544-18545 HYPH denotes /
T7880 18545-18546 SYM denotes -
T7876 18546-18557 NNS denotes macrophages
T7881 18558-18563 MD denotes could
T7882 18564-18566 VB denotes be
T7873 18567-18577 VBN denotes identified
T7883 18578-18582 WDT denotes that
T7885 18583-18586 VBD denotes had
T7884 18587-18595 VBN denotes engulfed
T7886 18596-18600 RB denotes even
T7887 18601-18605 JJR denotes more
T7889 18606-18615 JJ denotes apoptotic
T7890 18616-18622 NN denotes target
T7888 18623-18628 NNS denotes cells
T7891 18629-18633 IN denotes than
T7892 18634-18637 VBD denotes had
T7893 18638-18642 JJ denotes wild
T7895 18642-18643 HYPH denotes -
T7894 18643-18647 NN denotes type
T7896 18648-18659 NNS denotes macrophages
T7897 18660-18661 -LRB- denotes (
T7899 18661-18667 NN denotes Figure
T7900 18668-18670 NN denotes 7b
T7901 18670-18671 , denotes ,
T7898 18671-18672 NN denotes d
T7902 18672-18673 -RRB- denotes )
T7903 18673-18674 . denotes .
T7904 18674-18859 sentence denotes Thus, Ptdsr-deficient macrophages had a normal ability to ingest apoptotic cells and were not impaired in recognition or phagocytosis of cells that had undergone programmed cell death.
T7905 18675-18679 RB denotes Thus
T7907 18679-18681 , denotes ,
T7908 18681-18686 NN denotes Ptdsr
T7910 18686-18687 HYPH denotes -
T7909 18687-18696 JJ denotes deficient
T7911 18697-18708 NNS denotes macrophages
T7906 18709-18712 VBD denotes had
T7912 18713-18714 DT denotes a
T7914 18715-18721 JJ denotes normal
T7913 18722-18729 NN denotes ability
T7915 18730-18732 TO denotes to
T7916 18733-18739 VB denotes ingest
T7917 18740-18749 JJ denotes apoptotic
T7918 18750-18755 NNS denotes cells
T7919 18756-18759 CC denotes and
T7920 18760-18764 VBD denotes were
T7922 18765-18768 RB denotes not
T7921 18769-18777 VBN denotes impaired
T7923 18778-18780 IN denotes in
T7924 18781-18792 NN denotes recognition
T7925 18793-18795 CC denotes or
T7926 18796-18808 NN denotes phagocytosis
T7927 18809-18811 IN denotes of
T7928 18812-18817 NNS denotes cells
T7929 18818-18822 WDT denotes that
T7931 18823-18826 VBD denotes had
T7930 18827-18836 VBN denotes undergone
T7932 18837-18847 VBN denotes programmed
T7934 18848-18852 NN denotes cell
T7933 18853-18858 NN denotes death
T7935 18858-18859 . denotes .
T8339 18861-18866 NN denotes Ptdsr
T8341 18866-18867 HYPH denotes -
T8340 18867-18877 NN denotes deficiency
T8342 18878-18885 VBZ denotes results
T8343 18886-18888 IN denotes in
T8344 18889-18896 VBN denotes reduced
T8345 18897-18907 NN denotes production
T8346 18908-18910 IN denotes of
T8347 18911-18914 JJ denotes pro
T8349 18914-18915 HYPH denotes -
T8350 18916-18919 CC denotes and
T8351 18920-18937 JJ denotes anti-inflammatory
T8348 18938-18947 NNS denotes cytokines
T8352 18948-18953 IN denotes after
T8353 18954-18964 NN denotes macrophage
T8354 18965-18976 NN denotes stimulation
T8355 18976-19237 sentence denotes In addition to its suggested importance for phagocytosis of apoptotic cells, it has been proposed that Ptdsr fulfils a second crucial role in regulating and maintaining a non-inflammatory environment upon the recognition of apoptotic cells by macrophages [26].
T8356 18977-18979 IN denotes In
T8358 18980-18988 NN denotes addition
T8359 18989-18991 IN denotes to
T8360 18992-18995 PRP$ denotes its
T8362 18996-19005 JJ denotes suggested
T8361 19006-19016 NN denotes importance
T8363 19017-19020 IN denotes for
T8364 19021-19033 NN denotes phagocytosis
T8365 19034-19036 IN denotes of
T8366 19037-19046 JJ denotes apoptotic
T8367 19047-19052 NNS denotes cells
T8368 19052-19054 , denotes ,
T8369 19054-19056 PRP denotes it
T8370 19057-19060 VBZ denotes has
T8371 19061-19065 VBN denotes been
T8357 19066-19074 VBN denotes proposed
T8372 19075-19079 IN denotes that
T8374 19080-19085 NN denotes Ptdsr
T8373 19086-19093 VBZ denotes fulfils
T8375 19094-19095 DT denotes a
T8377 19096-19102 JJ denotes second
T8378 19103-19110 JJ denotes crucial
T8376 19111-19115 NN denotes role
T8379 19116-19118 IN denotes in
T8380 19119-19129 VBG denotes regulating
T8381 19130-19133 CC denotes and
T8382 19134-19145 VBG denotes maintaining
T8383 19146-19147 DT denotes a
T8385 19148-19164 JJ denotes non-inflammatory
T8384 19165-19176 NN denotes environment
T8386 19177-19181 IN denotes upon
T8387 19182-19185 DT denotes the
T8388 19186-19197 NN denotes recognition
T8389 19198-19200 IN denotes of
T8390 19201-19210 JJ denotes apoptotic
T8391 19211-19216 NNS denotes cells
T8392 19217-19219 IN denotes by
T8393 19220-19231 NNS denotes macrophages
T8394 19232-19233 -LRB- denotes [
T8395 19233-19235 CD denotes 26
T8396 19235-19236 -RRB- denotes ]
T8397 19236-19237 . denotes .
T8398 19237-19372 sentence denotes We therefore tested whether Ptdsr -/- macrophages were able to release anti-inflammatory cytokines after ingestion of apoptotic cells.
T8399 19238-19240 PRP denotes We
T8401 19241-19250 RB denotes therefore
T8400 19251-19257 VBD denotes tested
T8402 19258-19265 IN denotes whether
T8404 19266-19271 NN denotes Ptdsr
T8406 19272-19273 SYM denotes -
T8407 19273-19274 HYPH denotes /
T8408 19274-19275 SYM denotes -
T8405 19276-19287 NNS denotes macrophages
T8403 19288-19292 VBD denotes were
T8409 19293-19297 JJ denotes able
T8410 19298-19300 TO denotes to
T8411 19301-19308 VB denotes release
T8412 19309-19326 JJ denotes anti-inflammatory
T8413 19327-19336 NNS denotes cytokines
T8414 19337-19342 IN denotes after
T8415 19343-19352 NN denotes ingestion
T8416 19353-19355 IN denotes of
T8417 19356-19365 JJ denotes apoptotic
T8418 19366-19371 NNS denotes cells
T8419 19371-19372 . denotes .
T8420 19372-19534 sentence denotes We examined levels of TGF-β1 and interleukin-10 (IL-10) after stimulation of FLDMs with lipopolysaccharide (LPS), with and without co-culture of apoptotic cells.
T8421 19373-19375 PRP denotes We
T8422 19376-19384 VBD denotes examined
T8423 19385-19391 NNS denotes levels
T8424 19392-19394 IN denotes of
T8425 19395-19398 NN denotes TGF
T8427 19398-19399 HYPH denotes -
T8426 19399-19401 NN denotes β1
T8428 19402-19405 CC denotes and
T8429 19406-19417 NN denotes interleukin
T8430 19417-19418 HYPH denotes -
T8431 19418-19420 CD denotes 10
T8432 19421-19422 -LRB- denotes (
T8433 19422-19424 NN denotes IL
T8434 19424-19425 HYPH denotes -
T8435 19425-19427 CD denotes 10
T8436 19427-19428 -RRB- denotes )
T8437 19429-19434 IN denotes after
T8438 19435-19446 NN denotes stimulation
T8439 19447-19449 IN denotes of
T8440 19450-19455 NNS denotes FLDMs
T8441 19456-19460 IN denotes with
T8442 19461-19479 NN denotes lipopolysaccharide
T8443 19480-19481 -LRB- denotes (
T8444 19481-19484 NN denotes LPS
T8445 19484-19485 -RRB- denotes )
T8446 19485-19487 , denotes ,
T8447 19487-19491 IN denotes with
T8448 19492-19495 CC denotes and
T8449 19496-19503 IN denotes without
T8450 19504-19514 NN denotes co-culture
T8451 19515-19517 IN denotes of
T8452 19518-19527 JJ denotes apoptotic
T8453 19528-19533 NNS denotes cells
T8454 19533-19534 . denotes .
T8455 19534-19797 sentence denotes Quantification of TGF-β1 and IL-10 levels after 22 hours of culture demonstrated that Ptdsr -/- macrophages were able to secrete these anti-inflammatory cytokines upon ingestion of apoptotic cells, although at a slightly lower level than wild-type (Figure 8a,b).
T8456 19535-19549 NN denotes Quantification
T8458 19550-19552 IN denotes of
T8459 19553-19556 NN denotes TGF
T8461 19556-19557 HYPH denotes -
T8460 19557-19559 NN denotes β1
T8463 19560-19563 CC denotes and
T8464 19564-19566 NN denotes IL
T8465 19566-19567 HYPH denotes -
T8466 19567-19569 CD denotes 10
T8462 19570-19576 NNS denotes levels
T8467 19577-19582 IN denotes after
T8468 19583-19585 CD denotes 22
T8469 19586-19591 NNS denotes hours
T8470 19592-19594 IN denotes of
T8471 19595-19602 NN denotes culture
T8457 19603-19615 VBD denotes demonstrated
T8472 19616-19620 IN denotes that
T8474 19621-19626 NN denotes Ptdsr
T8476 19627-19628 SYM denotes -
T8477 19628-19629 HYPH denotes /
T8478 19629-19630 SYM denotes -
T8475 19631-19642 NNS denotes macrophages
T8473 19643-19647 VBD denotes were
T8479 19648-19652 JJ denotes able
T8480 19653-19655 TO denotes to
T8481 19656-19663 VB denotes secrete
T8482 19664-19669 DT denotes these
T8484 19670-19687 JJ denotes anti-inflammatory
T8483 19688-19697 NNS denotes cytokines
T8485 19698-19702 IN denotes upon
T8486 19703-19712 NN denotes ingestion
T8487 19713-19715 IN denotes of
T8488 19716-19725 JJ denotes apoptotic
T8489 19726-19731 NNS denotes cells
T8490 19731-19733 , denotes ,
T8491 19733-19741 IN denotes although
T8492 19742-19744 IN denotes at
T8493 19745-19746 DT denotes a
T8495 19747-19755 RB denotes slightly
T8496 19756-19761 JJR denotes lower
T8494 19762-19767 NN denotes level
T8497 19768-19772 IN denotes than
T8498 19773-19777 JJ denotes wild
T8500 19777-19778 HYPH denotes -
T8499 19778-19782 NN denotes type
T8501 19783-19784 -LRB- denotes (
T8503 19784-19790 NN denotes Figure
T8504 19791-19793 NN denotes 8a
T8505 19793-19794 , denotes ,
T8502 19794-19795 NN denotes b
T8506 19795-19796 -RRB- denotes )
T8507 19796-19797 . denotes .
T8508 19797-19995 sentence denotes This indicates that ablation of Ptdsr function does not compromise in general the ability of macrophages to release immune-suppressive cytokines after recognition and engulfment of apoptotic cells.
T8509 19798-19802 DT denotes This
T8510 19803-19812 VBZ denotes indicates
T8511 19813-19817 IN denotes that
T8513 19818-19826 NN denotes ablation
T8514 19827-19829 IN denotes of
T8515 19830-19835 NN denotes Ptdsr
T8516 19836-19844 NN denotes function
T8517 19845-19849 VBZ denotes does
T8518 19850-19853 RB denotes not
T8512 19854-19864 VB denotes compromise
T8519 19865-19867 IN denotes in
T8520 19868-19875 JJ denotes general
T8521 19876-19879 DT denotes the
T8522 19880-19887 NN denotes ability
T8523 19888-19890 IN denotes of
T8524 19891-19902 NNS denotes macrophages
T8525 19903-19905 TO denotes to
T8526 19906-19913 VB denotes release
T8527 19914-19920 NN denotes immune
T8529 19920-19921 HYPH denotes -
T8528 19921-19932 JJ denotes suppressive
T8530 19933-19942 NNS denotes cytokines
T8531 19943-19948 IN denotes after
T8532 19949-19960 NN denotes recognition
T8533 19961-19964 CC denotes and
T8534 19965-19975 NN denotes engulfment
T8535 19976-19978 IN denotes of
T8536 19979-19988 JJ denotes apoptotic
T8537 19989-19994 NNS denotes cells
T8538 19994-19995 . denotes .
T8539 19995-20252 sentence denotes To analyze whether pro-inflammatory signaling is affected in Ptdsr -/- macrophages, we stimulated FLDMs from Ptdsr+/+ and Ptdsr -/- mice with LPS and measured levels of tumor necrosis factor-α (TNF-α) at different time points after stimulation (Figure 8c).
T8540 19996-19998 TO denotes To
T8541 19999-20006 VB denotes analyze
T8543 20007-20014 IN denotes whether
T8545 20015-20031 JJ denotes pro-inflammatory
T8546 20032-20041 NN denotes signaling
T8547 20042-20044 VBZ denotes is
T8544 20045-20053 VBN denotes affected
T8548 20054-20056 IN denotes in
T8549 20057-20062 NN denotes Ptdsr
T8551 20063-20064 SYM denotes -
T8552 20064-20065 HYPH denotes /
T8553 20065-20066 SYM denotes -
T8550 20067-20078 NNS denotes macrophages
T8554 20078-20080 , denotes ,
T8555 20080-20082 PRP denotes we
T8542 20083-20093 VBD denotes stimulated
T8556 20094-20099 NNS denotes FLDMs
T8557 20100-20104 IN denotes from
T8558 20105-20110 NN denotes Ptdsr
T8560 20110-20111 SYM denotes +
T8561 20111-20112 HYPH denotes /
T8562 20112-20113 SYM denotes +
T8563 20114-20117 CC denotes and
T8564 20118-20123 NN denotes Ptdsr
T8565 20124-20125 SYM denotes -
T8566 20125-20126 HYPH denotes /
T8567 20126-20127 SYM denotes -
T8559 20128-20132 NNS denotes mice
T8568 20133-20137 IN denotes with
T8569 20138-20141 NN denotes LPS
T8570 20142-20145 CC denotes and
T8571 20146-20154 VBD denotes measured
T8572 20155-20161 NNS denotes levels
T8573 20162-20164 IN denotes of
T8574 20165-20170 NN denotes tumor
T8576 20171-20179 NN denotes necrosis
T8577 20180-20186 NN denotes factor
T8578 20186-20187 HYPH denotes -
T8575 20187-20188 NN denotes α
T8579 20189-20190 -LRB- denotes (
T8581 20190-20193 NN denotes TNF
T8582 20193-20194 HYPH denotes -
T8580 20194-20195 NN denotes α
T8583 20195-20196 -RRB- denotes )
T8584 20197-20199 IN denotes at
T8585 20200-20209 JJ denotes different
T8587 20210-20214 NN denotes time
T8586 20215-20221 NNS denotes points
T8588 20222-20227 IN denotes after
T8589 20228-20239 NN denotes stimulation
T8590 20240-20241 -LRB- denotes (
T8592 20241-20247 NN denotes Figure
T8591 20248-20250 NN denotes 8c
T8593 20250-20251 -RRB- denotes )
T8594 20251-20252 . denotes .
T8595 20252-20340 sentence denotes Ptdsr -/- macrophages produced significantly less TNF-α than did wild-type macrophages.
T8596 20253-20258 NN denotes Ptdsr
T8598 20259-20260 SYM denotes -
T8599 20260-20261 HYPH denotes /
T8600 20261-20262 SYM denotes -
T8597 20263-20274 NNS denotes macrophages
T8601 20275-20283 VBD denotes produced
T8602 20284-20297 RB denotes significantly
T8603 20298-20302 JJR denotes less
T8605 20303-20306 NN denotes TNF
T8606 20306-20307 HYPH denotes -
T8604 20307-20308 NN denotes α
T8607 20309-20313 IN denotes than
T8608 20314-20317 VBD denotes did
T8609 20318-20322 JJ denotes wild
T8611 20322-20323 HYPH denotes -
T8610 20323-20327 NN denotes type
T8612 20328-20339 NNS denotes macrophages
T8613 20339-20340 . denotes .
T8614 20340-20548 sentence denotes The difference in TNF-α secretion was first visible after 3 h of LPS stimulation and became more prominent during the course of the experiment (for example, after 9 h and 12 h of LPS stimulation; Figure 8c).
T8615 20341-20344 DT denotes The
T8616 20345-20355 NN denotes difference
T8618 20356-20358 IN denotes in
T8619 20359-20362 NN denotes TNF
T8621 20362-20363 HYPH denotes -
T8620 20363-20364 NN denotes α
T8622 20365-20374 NN denotes secretion
T8617 20375-20378 VBD denotes was
T8623 20379-20384 RB denotes first
T8624 20385-20392 JJ denotes visible
T8625 20393-20398 IN denotes after
T8626 20399-20400 CD denotes 3
T8627 20401-20402 NNS denotes h
T8628 20403-20405 IN denotes of
T8629 20406-20409 NN denotes LPS
T8630 20410-20421 NN denotes stimulation
T8631 20422-20425 CC denotes and
T8632 20426-20432 VBD denotes became
T8633 20433-20437 RBR denotes more
T8634 20438-20447 JJ denotes prominent
T8635 20448-20454 IN denotes during
T8636 20455-20458 DT denotes the
T8637 20459-20465 NN denotes course
T8638 20466-20468 IN denotes of
T8639 20469-20472 DT denotes the
T8640 20473-20483 NN denotes experiment
T8641 20484-20485 -LRB- denotes (
T8643 20485-20488 IN denotes for
T8644 20489-20496 NN denotes example
T8645 20496-20498 , denotes ,
T8646 20498-20503 IN denotes after
T8647 20504-20505 CD denotes 9
T8648 20506-20507 NNS denotes h
T8649 20508-20511 CC denotes and
T8650 20512-20514 CD denotes 12
T8651 20515-20516 NNS denotes h
T8652 20517-20519 IN denotes of
T8653 20520-20523 NN denotes LPS
T8654 20524-20535 NN denotes stimulation
T8655 20535-20536 : denotes ;
T8656 20537-20543 NN denotes Figure
T8642 20544-20546 NN denotes 8c
T8657 20546-20547 -RRB- denotes )
T8658 20547-20548 . denotes .
T8659 20548-20711 sentence denotes To analyze whether TNF-α release by Ptdsr -/- macrophages can be affected by engulfment of apoptotic cells, we stimulated FLDMs with LPS, apoptotic cells or both.
T8660 20549-20551 TO denotes To
T8661 20552-20559 VB denotes analyze
T8663 20560-20567 IN denotes whether
T8665 20568-20571 NN denotes TNF
T8667 20571-20572 HYPH denotes -
T8666 20572-20573 NN denotes α
T8668 20574-20581 NN denotes release
T8669 20582-20584 IN denotes by
T8670 20585-20590 NN denotes Ptdsr
T8672 20591-20592 SYM denotes -
T8673 20592-20593 HYPH denotes /
T8674 20593-20594 SYM denotes -
T8671 20595-20606 NNS denotes macrophages
T8675 20607-20610 MD denotes can
T8676 20611-20613 VB denotes be
T8664 20614-20622 VBN denotes affected
T8677 20623-20625 IN denotes by
T8678 20626-20636 NN denotes engulfment
T8679 20637-20639 IN denotes of
T8680 20640-20649 JJ denotes apoptotic
T8681 20650-20655 NNS denotes cells
T8682 20655-20657 , denotes ,
T8683 20657-20659 PRP denotes we
T8662 20660-20670 VBD denotes stimulated
T8684 20671-20676 NNS denotes FLDMs
T8685 20677-20681 IN denotes with
T8686 20682-20685 NN denotes LPS
T8687 20685-20687 , denotes ,
T8688 20687-20696 JJ denotes apoptotic
T8689 20697-20702 NNS denotes cells
T8690 20703-20705 CC denotes or
T8691 20706-20710 DT denotes both
T8692 20710-20711 . denotes .
T8693 20711-20946 sentence denotes Quantification of TNF-α levels by ELISA after 22 h showed that Ptdsr-deficient macrophages release less TNF-α after stimulation with LPS alone, and also after double stimulation of macrophages with LPS and apoptotic cells (Figure 8d).
T8694 20712-20726 NN denotes Quantification
T8696 20727-20729 IN denotes of
T8697 20730-20733 NN denotes TNF
T8699 20733-20734 HYPH denotes -
T8698 20734-20735 NN denotes α
T8700 20736-20742 NNS denotes levels
T8701 20743-20745 IN denotes by
T8702 20746-20751 NN denotes ELISA
T8703 20752-20757 IN denotes after
T8704 20758-20760 CD denotes 22
T8705 20761-20762 NN denotes h
T8695 20763-20769 VBD denotes showed
T8706 20770-20774 IN denotes that
T8708 20775-20780 NN denotes Ptdsr
T8710 20780-20781 HYPH denotes -
T8709 20781-20790 JJ denotes deficient
T8711 20791-20802 NNS denotes macrophages
T8707 20803-20810 VBP denotes release
T8712 20811-20815 JJR denotes less
T8714 20816-20819 NN denotes TNF
T8715 20819-20820 HYPH denotes -
T8713 20820-20821 NN denotes α
T8716 20822-20827 IN denotes after
T8717 20828-20839 NN denotes stimulation
T8718 20840-20844 IN denotes with
T8719 20845-20848 NN denotes LPS
T8720 20849-20854 RB denotes alone
T8721 20854-20856 , denotes ,
T8722 20856-20859 CC denotes and
T8723 20860-20864 RB denotes also
T8724 20865-20870 IN denotes after
T8725 20871-20877 JJ denotes double
T8726 20878-20889 NN denotes stimulation
T8727 20890-20892 IN denotes of
T8728 20893-20904 NNS denotes macrophages
T8729 20905-20909 IN denotes with
T8730 20910-20913 NN denotes LPS
T8731 20914-20917 CC denotes and
T8732 20918-20927 JJ denotes apoptotic
T8733 20928-20933 NNS denotes cells
T8734 20934-20935 -LRB- denotes (
T8736 20935-20941 NN denotes Figure
T8735 20942-20944 NN denotes 8d
T8737 20944-20945 -RRB- denotes )
T8738 20945-20946 . denotes .
T8739 20946-21174 sentence denotes Moreover, the double stimulation demonstrated that the LPS-induced TNF-α release by Ptdsr -/- macrophages could be inhibited by co-administration of apoptotic cells to an extent comparable to that seen in wild-type macrophages.
T8740 20947-20955 RB denotes Moreover
T8742 20955-20957 , denotes ,
T8743 20957-20960 DT denotes the
T8745 20961-20967 JJ denotes double
T8744 20968-20979 NN denotes stimulation
T8741 20980-20992 VBD denotes demonstrated
T8746 20993-20997 IN denotes that
T8748 20998-21001 DT denotes the
T8750 21002-21005 NN denotes LPS
T8752 21005-21006 HYPH denotes -
T8751 21006-21013 VBN denotes induced
T8753 21014-21017 NN denotes TNF
T8755 21017-21018 HYPH denotes -
T8754 21018-21019 NN denotes α
T8749 21020-21027 NN denotes release
T8756 21028-21030 IN denotes by
T8757 21031-21036 NN denotes Ptdsr
T8759 21037-21038 SYM denotes -
T8760 21038-21039 HYPH denotes /
T8761 21039-21040 SYM denotes -
T8758 21041-21052 NNS denotes macrophages
T8762 21053-21058 MD denotes could
T8763 21059-21061 VB denotes be
T8747 21062-21071 VBN denotes inhibited
T8764 21072-21074 IN denotes by
T8765 21075-21092 NN denotes co-administration
T8766 21093-21095 IN denotes of
T8767 21096-21105 JJ denotes apoptotic
T8768 21106-21111 NNS denotes cells
T8769 21112-21114 IN denotes to
T8770 21115-21117 DT denotes an
T8771 21118-21124 NN denotes extent
T8772 21125-21135 JJ denotes comparable
T8773 21136-21138 IN denotes to
T8774 21139-21143 DT denotes that
T8775 21144-21148 VBN denotes seen
T8776 21149-21151 IN denotes in
T8777 21152-21156 JJ denotes wild
T8779 21156-21157 HYPH denotes -
T8778 21157-21161 NN denotes type
T8780 21162-21173 NNS denotes macrophages
T8781 21173-21174 . denotes .
T8782 21174-21337 sentence denotes Similar results were obtained when other pro-inflammatory cytokines, such as interleukin-6 and monocyte chemoattractant protein-1, were analyzed (data not shown).
T8783 21175-21182 JJ denotes Similar
T8784 21183-21190 NNS denotes results
T8786 21191-21195 VBD denotes were
T8785 21196-21204 VBN denotes obtained
T8787 21205-21209 WRB denotes when
T8789 21210-21215 JJ denotes other
T8791 21216-21232 JJ denotes pro-inflammatory
T8790 21233-21242 NNS denotes cytokines
T8792 21242-21244 , denotes ,
T8793 21244-21248 JJ denotes such
T8794 21249-21251 IN denotes as
T8795 21252-21263 NN denotes interleukin
T8796 21263-21264 HYPH denotes -
T8797 21264-21265 CD denotes 6
T8798 21266-21269 CC denotes and
T8799 21270-21278 NN denotes monocyte
T8801 21279-21294 NN denotes chemoattractant
T8800 21295-21302 NN denotes protein
T8802 21302-21303 HYPH denotes -
T8803 21303-21304 CD denotes 1
T8804 21304-21306 , denotes ,
T8805 21306-21310 VBD denotes were
T8788 21311-21319 VBN denotes analyzed
T8806 21320-21321 -LRB- denotes (
T8808 21321-21325 NNS denotes data
T8809 21326-21329 RB denotes not
T8807 21330-21335 VBN denotes shown
T8810 21335-21336 -RRB- denotes )
T8811 21336-21337 . denotes .
T8812 21337-21504 sentence denotes These results indicate that Ptdsr is not required in macrophages for the inhibition of pro-inflammatory signaling after recognition and engulfment of apoptotic cells.
T8813 21338-21343 DT denotes These
T8814 21344-21351 NNS denotes results
T8815 21352-21360 VBP denotes indicate
T8816 21361-21365 IN denotes that
T8818 21366-21371 NN denotes Ptdsr
T8819 21372-21374 VBZ denotes is
T8820 21375-21378 RB denotes not
T8817 21379-21387 VBN denotes required
T8821 21388-21390 IN denotes in
T8822 21391-21402 NNS denotes macrophages
T8823 21403-21406 IN denotes for
T8824 21407-21410 DT denotes the
T8825 21411-21421 NN denotes inhibition
T8826 21422-21424 IN denotes of
T8827 21425-21441 JJ denotes pro-inflammatory
T8828 21442-21451 NN denotes signaling
T8829 21452-21457 IN denotes after
T8830 21458-21469 NN denotes recognition
T8831 21470-21473 CC denotes and
T8832 21474-21484 NN denotes engulfment
T8833 21485-21487 IN denotes of
T8834 21488-21497 JJ denotes apoptotic
T8835 21498-21503 NNS denotes cells
T8836 21503-21504 . denotes .
T8837 21504-21815 sentence denotes Ptdsr-deficiency does, however, affect the overall release of pro- and anti-inflammatory cytokines after stimulation with LPS and after double treatment with LPS and apoptotic cells, indicating that Ptdsr-deficient macrophages have a reduced capacity to produce or secrete pro- and anti-inflammatory cytokines.
T8838 21505-21510 NN denotes Ptdsr
T8840 21510-21511 HYPH denotes -
T8839 21511-21521 NN denotes deficiency
T8842 21522-21526 VBZ denotes does
T8843 21526-21528 , denotes ,
T8844 21528-21535 RB denotes however
T8845 21535-21537 , denotes ,
T8841 21537-21543 VB denotes affect
T8846 21544-21547 DT denotes the
T8848 21548-21555 JJ denotes overall
T8847 21556-21563 NN denotes release
T8849 21564-21566 IN denotes of
T8850 21567-21570 JJ denotes pro
T8852 21570-21571 HYPH denotes -
T8853 21572-21575 CC denotes and
T8854 21576-21593 JJ denotes anti-inflammatory
T8851 21594-21603 NNS denotes cytokines
T8855 21604-21609 IN denotes after
T8856 21610-21621 NN denotes stimulation
T8857 21622-21626 IN denotes with
T8858 21627-21630 NN denotes LPS
T8859 21631-21634 CC denotes and
T8860 21635-21640 IN denotes after
T8861 21641-21647 JJ denotes double
T8862 21648-21657 NN denotes treatment
T8863 21658-21662 IN denotes with
T8864 21663-21666 NN denotes LPS
T8865 21667-21670 CC denotes and
T8866 21671-21680 JJ denotes apoptotic
T8867 21681-21686 NNS denotes cells
T8868 21686-21688 , denotes ,
T8869 21688-21698 VBG denotes indicating
T8870 21699-21703 IN denotes that
T8872 21704-21709 NN denotes Ptdsr
T8874 21709-21710 HYPH denotes -
T8873 21710-21719 JJ denotes deficient
T8875 21720-21731 NNS denotes macrophages
T8871 21732-21736 VBP denotes have
T8876 21737-21738 DT denotes a
T8878 21739-21746 VBN denotes reduced
T8877 21747-21755 NN denotes capacity
T8879 21756-21758 TO denotes to
T8880 21759-21766 VB denotes produce
T8881 21767-21769 CC denotes or
T8882 21770-21777 VB denotes secrete
T8883 21778-21781 JJ denotes pro
T8885 21781-21782 HYPH denotes -
T8886 21783-21786 CC denotes and
T8887 21787-21804 JJ denotes anti-inflammatory
T8884 21805-21814 NNS denotes cytokines
T8888 21814-21815 . denotes .
R1344 T2693 T2692 prep of,Generation
R1345 T2694 T2695 npadvmod Ptdsr,deficient
R1346 T2695 T2697 amod deficient,mice
R1347 T2696 T2695 punct -,deficient
R1348 T2697 T2693 pobj mice,of
R1349 T2699 T2700 aux To,investigate
R1350 T2700 T2701 advcl investigate,generated
R1351 T2702 T2703 advmod in,vivo
R1352 T2703 T2700 advmod vivo,investigate
R1353 T2704 T2705 det the,functions
R1354 T2705 T2700 dobj functions,investigate
R1355 T2706 T2705 prep of,functions
R1356 T2707 T2708 det the,receptor
R1357 T2708 T2706 pobj receptor,of
R1358 T2709 T2708 compound phosphatidylserine,receptor
R1359 T2710 T2708 appos Ptdsr,receptor
R1360 T2711 T2701 punct ", ",generated
R1361 T2712 T2701 nsubj we,generated
R1362 T2713 T2714 det a,allele
R1363 T2714 T2701 dobj allele,generated
R1364 T2715 T2714 amod null,allele
R1365 T2716 T2701 prep in,generated
R1366 T2717 T2718 det the,mouse
R1367 T2718 T2716 pobj mouse,in
R1368 T2719 T2701 prep by,generated
R1369 T2720 T2721 compound gene,targeting
R1370 T2721 T2719 pobj targeting,by
R1371 T2722 T2723 punct (,1c
R1372 T2723 T2701 parataxis 1c,generated
R1373 T2724 T2723 nmod Figure,1c
R1374 T2725 T2723 nmod 1a,1c
R1375 T2726 T2723 punct ",",1c
R1376 T2727 T2723 nmod 1b,1c
R1377 T2728 T2723 punct ",",1c
R1378 T2729 T2723 punct ),1c
R1379 T2730 T2701 punct .,generated
R1380 T2732 T2733 prep In,used
R1381 T2734 T2732 pobj contrast,In
R1382 T2735 T2734 prep to,contrast
R1383 T2736 T2737 advmod previously,described
R1384 T2737 T2738 amod described,mice
R1385 T2738 T2735 pobj mice,to
R1386 T2739 T2740 compound Ptdsr,knockout
R1387 T2740 T2738 compound knockout,mice
R1388 T2741 T2740 punct -,knockout
R1389 T2742 T2743 punct [,32
R1390 T2743 T2732 parataxis 32,In
R1391 T2744 T2743 nummod 31,32
R1392 T2745 T2743 punct ",",32
R1393 T2746 T2743 punct ],32
R1394 T2747 T2733 punct ", ",used
R1395 T2748 T2733 nsubj we,used
R1396 T2749 T2750 nmod Bruce4,cells
R1397 T2750 T2733 dobj cells,used
R1398 T2751 T2752 amod embryonic,stem
R1399 T2752 T2750 nmod stem,cells
R1400 T2753 T2752 punct (,stem
R1401 T2754 T2752 appos ES,stem
R1402 T2755 T2752 punct ),stem
R1403 T2756 T2733 prep for,used
R1404 T2757 T2758 compound gene,targeting
R1405 T2758 T2756 pobj targeting,for
R1406 T2759 T2760 punct [,33
R1407 T2760 T2733 parataxis 33,used
R1408 T2761 T2760 punct ],33
R1409 T2762 T2733 punct ", ",used
R1410 T2763 T2764 advmod thus,generating
R1411 T2764 T2733 advcl generating,used
R1412 T2765 T2766 det a,allele
R1413 T2766 T2764 dobj allele,generating
R1414 T2767 T2768 npadvmod Ptdsr,null
R1415 T2768 T2766 amod null,allele
R1416 T2769 T2768 punct -,null
R1417 T2770 T2764 prep in,generating
R1418 T2771 T2772 det a,background
R1419 T2772 T2770 pobj background,in
R1420 T2773 T2772 amod pure,background
R1421 T2774 T2772 punct ", ",background
R1422 T2775 T2772 amod isogenic,background
R1423 T2776 T2777 nmod C57BL,6J
R1424 T2777 T2772 nmod 6J,background
R1425 T2778 T2777 punct /,6J
R1426 T2779 T2772 amod genetic,background
R1427 T2780 T2733 punct .,used
R1428 T2782 T2783 det The,line
R1429 T2783 T2788 nsubjpass line,named
R1430 T2784 T2785 advmod newly,established
R1431 T2785 T2783 amod established,line
R1432 T2786 T2783 compound knockout,line
R1433 T2787 T2783 compound mouse,line
R1434 T2789 T2788 auxpass was,named
R1435 T2790 T2788 oprd Ptdsrtm1Gbf,named
R1436 T2791 T2792 punct (,referred
R1437 T2792 T2790 parataxis referred,Ptdsrtm1Gbf
R1438 T2793 T2792 advmod hereafter,referred
R1439 T2794 T2792 prep to,referred
R1440 T2795 T2792 prep as,referred
R1441 T2796 T2795 pobj Ptdsr,as
R1442 T2797 T2796 punct -,Ptdsr
R1443 T2798 T2796 punct /,Ptdsr
R1444 T2799 T2796 punct -,Ptdsr
R1445 T2800 T2792 punct ),referred
R1446 T2801 T2788 punct .,named
R1447 T2803 T2804 amod Heterozygous,mice
R1448 T2804 T2809 nsubj mice,were
R1449 T2805 T2804 nmod Ptdsr,mice
R1450 T2806 T2805 punct +,Ptdsr
R1451 T2807 T2805 punct /,Ptdsr
R1452 T2808 T2805 punct -,Ptdsr
R1453 T2810 T2809 acomp viable,were
R1454 T2811 T2810 cc and,viable
R1455 T2812 T2810 conj fertile,viable
R1456 T2813 T2809 cc and,were
R1457 T2814 T2809 conj showed,were
R1458 T2815 T2816 det no,abnormalities
R1459 T2816 T2814 dobj abnormalities,showed
R1460 T2817 T2816 amod obvious,abnormalities
R1461 T2818 T2809 punct .,were
R1462 T2820 T2821 nmod Ptdsr,mice
R1463 T2821 T2825 nsubjpass mice,intercrossed
R1464 T2822 T2820 punct +,Ptdsr
R1465 T2823 T2820 punct /,Ptdsr
R1466 T2824 T2820 punct -,Ptdsr
R1467 T2826 T2825 auxpass were,intercrossed
R1468 T2827 T2828 aux to,generate
R1469 T2828 T2825 advcl generate,intercrossed
R1470 T2829 T2830 amod homozygous,mice
R1471 T2830 T2828 dobj mice,generate
R1472 T2831 T2832 npadvmod Ptdsr,deficient
R1473 T2832 T2830 amod deficient,mice
R1474 T2833 T2832 punct -,deficient
R1475 T2834 T2825 punct .,intercrossed
R1476 T2836 T2837 det The,absence
R1477 T2837 T2838 nsubjpass absence,confirmed
R1478 T2839 T2837 prep of,absence
R1479 T2840 T2841 compound Ptdsr,expression
R1480 T2841 T2839 pobj expression,of
R1481 T2842 T2837 prep in,absence
R1482 T2843 T2844 nmod Ptdsr,embryos
R1483 T2844 T2842 pobj embryos,in
R1484 T2845 T2843 punct -,Ptdsr
R1485 T2846 T2843 punct /,Ptdsr
R1486 T2847 T2843 punct -,Ptdsr
R1487 T2848 T2838 auxpass was,confirmed
R1488 T2849 T2838 prep by,confirmed
R1489 T2850 T2851 compound RT,PCR
R1490 T2851 T2849 pobj PCR,by
R1491 T2852 T2851 punct -,PCR
R1492 T2853 T2854 punct (,shown
R1493 T2854 T2849 parataxis shown,by
R1494 T2855 T2854 nsubj data,shown
R1495 T2856 T2854 neg not,shown
R1496 T2857 T2854 punct ),shown
R1497 T2858 T2849 punct ", ",by
R1498 T2859 T2849 cc and,by
R1499 T2860 T2849 conj by,by
R1500 T2861 T2862 nmod northern,blotting
R1501 T2862 T2865 compound blotting,analyses
R1502 T2863 T2861 cc and,northern
R1503 T2864 T2861 conj western,northern
R1504 T2865 T2860 pobj analyses,by
R1505 T2866 T2867 punct (,e
R1506 T2867 T2838 parataxis e,confirmed
R1507 T2868 T2867 nmod Figure,e
R1508 T2869 T2867 nmod 1d,e
R1509 T2870 T2867 punct ",",e
R1510 T2871 T2867 punct ),e
R1511 T2872 T2838 punct .,confirmed
R1512 T2874 T2875 nsubj Interbreeding,showed
R1513 T2876 T2874 prep of,Interbreeding
R1514 T2877 T2878 amod heterozygous,mice
R1515 T2878 T2876 pobj mice,of
R1516 T2879 T2880 mark that,was
R1517 T2880 T2875 ccomp was,showed
R1518 T2881 T2882 det the,mutation
R1519 T2882 T2880 nsubj mutation,was
R1520 T2883 T2880 acomp lethal,was
R1521 T2884 T2880 punct ", ",was
R1522 T2885 T2886 mark since,detected
R1523 T2886 T2880 advcl detected,was
R1524 T2887 T2888 amod homozygous,mutants
R1525 T2888 T2886 nsubjpass mutants,detected
R1526 T2889 T2886 auxpass were,detected
R1527 T2890 T2886 neg not,detected
R1528 T2891 T2886 prep in,detected
R1529 T2892 T2893 quantmod over,100
R1530 T2893 T2894 nummod 100,litters
R1531 T2894 T2891 pobj litters,in
R1532 T2895 T2894 amod analyzed,litters
R1533 T2896 T2886 prep at,detected
R1534 T2897 T2896 pobj weaning,at
R1535 T2898 T2875 punct .,showed
R1536 T2900 T2901 aux To,determine
R1537 T2901 T2902 advcl determine,followed
R1538 T2903 T2904 det the,stages
R1539 T2904 T2901 dobj stages,determine
R1540 T2905 T2904 prep of,stages
R1541 T2906 T2907 amod embryonic,development
R1542 T2907 T2905 pobj development,of
R1543 T2908 T2907 acl affected,development
R1544 T2909 T2908 agent by,affected
R1545 T2910 T2911 det the,mutation
R1546 T2911 T2909 pobj mutation,by
R1547 T2912 T2911 compound Ptdsrtm1Gbf,mutation
R1548 T2913 T2902 punct ", ",followed
R1549 T2914 T2915 amod timed,breedings
R1550 T2915 T2902 nsubjpass breedings,followed
R1551 T2916 T2902 auxpass were,followed
R1552 T2917 T2902 prep by,followed
R1553 T2918 T2919 compound PCR,genotyping
R1554 T2919 T2917 pobj genotyping,by
R1555 T2920 T2921 punct (,1c
R1556 T2921 T2919 parataxis 1c,genotyping
R1557 T2922 T2921 compound Figure,1c
R1558 T2923 T2921 punct ),1c
R1559 T2924 T2919 prep of,genotyping
R1560 T2925 T2924 pobj embryos,of
R1561 T2926 T2902 punct .,followed
R1562 T2928 T2929 nsubj We,recovered
R1563 T2930 T2929 dobj fewer,recovered
R1564 T2931 T2930 prep than,fewer
R1565 T2932 T2933 det the,number
R1566 T2933 T2931 pobj number,than
R1567 T2934 T2933 amod expected,number
R1568 T2935 T2933 prep of,number
R1569 T2936 T2937 amod homozygous,embryos
R1570 T2937 T2935 pobj embryos,of
R1571 T2938 T2929 prep from,recovered
R1572 T2939 T2938 pobj intercrosses,from
R1573 T2940 T2939 prep of,intercrosses
R1574 T2941 T2942 nmod Ptdsr,mice
R1575 T2942 T2940 pobj mice,of
R1576 T2943 T2941 punct +,Ptdsr
R1577 T2944 T2941 punct /,Ptdsr
R1578 T2945 T2941 punct -,Ptdsr
R1579 T2946 T2929 punct .,recovered
R1580 T2948 T2949 prep From,harvested
R1581 T2950 T2951 det a,total
R1582 T2951 T2948 pobj total,From
R1583 T2952 T2951 prep of,total
R1584 T2953 T2954 nummod "1,031",embryos
R1585 T2954 T2952 pobj embryos,of
R1586 T2955 T2954 acl analyzed,embryos
R1587 T2956 T2955 prep between,analyzed
R1588 T2957 T2958 amod gestational,E
R1589 T2958 T2956 pobj E,between
R1590 T2959 T2958 nmod day,E
R1591 T2960 T2958 punct (,E
R1592 T2961 T2958 punct ),E
R1593 T2962 T2958 nummod 9.5,E
R1594 T2963 T2958 cc and,E
R1595 T2964 T2958 conj E18.5,E
R1596 T2965 T2949 punct ", ",harvested
R1597 T2966 T2967 nummod 198,embryos
R1598 T2967 T2949 nsubjpass embryos,harvested
R1599 T2968 T2969 punct (,%
R1600 T2969 T2966 parataxis %,198
R1601 T2970 T2969 nummod 19.2,%
R1602 T2971 T2969 punct ),%
R1603 T2972 T2973 npadvmod Ptdsr,deficient
R1604 T2973 T2967 amod deficient,embryos
R1605 T2974 T2973 punct -,deficient
R1606 T2975 T2967 amod homozygous,embryos
R1607 T2976 T2949 auxpass were,harvested
R1608 T2977 T2949 punct ", ",harvested
R1609 T2978 T2949 advcl indicating,harvested
R1610 T2979 T2980 mark that,associated
R1611 T2980 T2978 ccomp associated,indicating
R1612 T2981 T2982 det the,mutation
R1613 T2982 T2980 nsubjpass mutation,associated
R1614 T2983 T2982 amod introduced,mutation
R1615 T2984 T2980 auxpass is,associated
R1616 T2985 T2980 prep with,associated
R1617 T2986 T2987 det a,rate
R1618 T2987 T2985 pobj rate,with
R1619 T2988 T2987 amod low,rate
R1620 T2989 T2987 prep of,rate
R1621 T2990 T2991 amod embryonic,lethality
R1622 T2991 T2989 pobj lethality,of
R1623 T2992 T2993 advmod in,utero
R1624 T2993 T2991 advmod utero,lethality
R1625 T2994 T2949 punct .,harvested
R1626 T2996 T2997 prep From,were
R1627 T2998 T2996 pobj E9.5,From
R1628 T2999 T2996 prep to,From
R1629 T3000 T2999 pobj E12.5,to
R1630 T3001 T2997 punct ", ",were
R1631 T3002 T3003 nmod Ptdsr,embryos
R1632 T3003 T2997 nsubj embryos,were
R1633 T3004 T3002 punct -,Ptdsr
R1634 T3005 T3002 punct /,Ptdsr
R1635 T3006 T3002 punct -,Ptdsr
R1636 T3007 T2997 acomp viable,were
R1637 T3008 T3007 cc and,viable
R1638 T3009 T3007 conj of,viable
R1639 T3010 T3011 amod normal,size
R1640 T3011 T3009 pobj size,of
R1641 T3012 T2997 punct .,were
R1642 T3014 T3015 prep At,showed
R1643 T3016 T3014 pobj E13.5,At
R1644 T3017 T3014 cc and,At
R1645 T3018 T3014 conj thereafter,At
R1646 T3019 T3015 punct ", ",showed
R1647 T3020 T3015 advmod however,showed
R1648 T3021 T3015 punct ", ",showed
R1649 T3022 T3023 amod most,embryos
R1650 T3023 T3015 nsubj embryos,showed
R1651 T3024 T3023 nmod Ptdsr,embryos
R1652 T3025 T3024 punct -,Ptdsr
R1653 T3026 T3024 punct /,Ptdsr
R1654 T3027 T3024 punct -,Ptdsr
R1655 T3028 T3029 amod morphological,abnormalities
R1656 T3029 T3015 dobj abnormalities,showed
R1657 T3030 T3031 punct (,Table
R1658 T3031 T3015 parataxis Table,showed
R1659 T3032 T3031 nummod 1,Table
R1660 T3033 T3031 punct ),Table
R1661 T3034 T3015 punct .,showed
R1662 T3036 T3037 det All,embryos
R1663 T3037 T3039 nsubj embryos,were
R1664 T3038 T3037 amod homozygous,embryos
R1665 T3040 T3037 acl harvested,embryos
R1666 T3041 T3042 npadvmod growth,retarded
R1667 T3042 T3039 acomp retarded,were
R1668 T3043 T3042 punct -,retarded
R1669 T3044 T3039 prep from,were
R1670 T3045 T3044 pobj E13.5,from
R1671 T3046 T3045 advmod onwards,E13.5
R1672 T3047 T3039 punct ", ",were
R1673 T3048 T3039 conj had,were
R1674 T3049 T3050 det a,appearance
R1675 T3050 T3048 dobj appearance,had
R1676 T3051 T3050 amod pale,appearance
R1677 T3052 T3048 punct ", ",had
R1678 T3053 T3048 cc and,had
R1679 T3054 T3048 conj displayed,had
R1680 T3055 T3056 amod multiple,dysmorphologies
R1681 T3056 T3054 dobj dysmorphologies,displayed
R1682 T3057 T3056 amod developmental,dysmorphologies
R1683 T3058 T3039 punct .,were
R1684 T3060 T3061 nsubj These,included
R1685 T3062 T3063 amod various,malformations
R1686 T3063 T3061 dobj malformations,included
R1687 T3064 T3063 nmod head,malformations
R1688 T3065 T3064 cc and,head
R1689 T3066 T3064 conj craniofacial,head
R1690 T3067 T3063 punct ", ",malformations
R1691 T3068 T3069 amod such,as
R1692 T3069 T3063 prep as,malformations
R1693 T3070 T3069 pobj exencephaly,as
R1694 T3071 T3070 punct ", ",exencephaly
R1695 T3072 T3073 amod cleft,palate
R1696 T3073 T3070 conj palate,exencephaly
R1697 T3074 T3073 cc and,palate
R1698 T3075 T3076 amod abnormal,shape
R1699 T3076 T3073 conj shape,palate
R1700 T3077 T3076 compound head,shape
R1701 T3078 T3079 punct (,g
R1702 T3079 T3061 parataxis g,included
R1703 T3080 T3079 nmod Figure,g
R1704 T3081 T3079 nmod 1f,g
R1705 T3082 T3079 punct ",",g
R1706 T3083 T3079 punct ),g
R1707 T3084 T3061 punct .,included
R1708 T3086 T3087 amod Gross,inspection
R1709 T3087 T3088 nsubj inspection,revealed
R1710 T3089 T3090 mark that,affected
R1711 T3090 T3088 ccomp affected,revealed
R1712 T3091 T3092 compound eye,development
R1713 T3092 T3090 nsubjpass development,affected
R1714 T3093 T3090 auxpass was,affected
R1715 T3094 T3090 advmod severely,affected
R1716 T3095 T3090 prep in,affected
R1717 T3096 T3097 nummod 14.1,%
R1718 T3097 T3095 pobj %,in
R1719 T3098 T3097 prep of,%
R1720 T3099 T3100 amod homozygous,embryos
R1721 T3100 T3098 pobj embryos,of
R1722 T3101 T3088 punct .,revealed
R1723 T3103 T3104 det The,animals
R1724 T3104 T3106 nsubj animals,displayed
R1725 T3105 T3104 amod affected,animals
R1726 T3107 T3108 det a,absence
R1727 T3108 T3106 dobj absence,displayed
R1728 T3109 T3108 amod complete,absence
R1729 T3110 T3108 amod unilateral,absence
R1730 T3111 T3110 cc or,unilateral
R1731 T3112 T3110 conj bilateral,unilateral
R1732 T3113 T3108 prep of,absence
R1733 T3114 T3115 det the,eyes
R1734 T3115 T3113 pobj eyes,of
R1735 T3116 T3117 punct (,Table
R1736 T3117 T3108 parataxis Table,absence
R1737 T3118 T3117 nummod 1,Table
R1738 T3119 T3117 punct ),Table
R1739 T3120 T3121 dep that,detected
R1740 T3121 T3108 relcl detected,absence
R1741 T3122 T3121 auxpass was,detected
R1742 T3123 T3121 neg never,detected
R1743 T3124 T3121 prep in,detected
R1744 T3125 T3126 nmod Ptdsr,littermates
R1745 T3126 T3124 pobj littermates,in
R1746 T3127 T3125 punct +,Ptdsr
R1747 T3128 T3125 punct /,Ptdsr
R1748 T3129 T3125 punct +,Ptdsr
R1749 T3130 T3125 cc or,Ptdsr
R1750 T3131 T3125 conj Ptdsr,Ptdsr
R1751 T3132 T3131 punct +,Ptdsr
R1752 T3133 T3131 punct /,Ptdsr
R1753 T3134 T3131 punct -,Ptdsr
R1754 T3135 T3106 punct .,displayed
R1755 T3137 T3138 advmod Furthermore,had
R1756 T3139 T3138 punct ", ",had
R1757 T3140 T3141 amod homozygous,embryos
R1758 T3141 T3138 nsubj embryos,had
R1759 T3142 T3141 acl harvested,embryos
R1760 T3143 T3142 prep between,harvested
R1761 T3144 T3143 pobj E12.5,between
R1762 T3145 T3144 cc and,E12.5
R1763 T3146 T3144 conj E15.5,E12.5
R1764 T3147 T3148 amod subcutaneous,edema
R1765 T3148 T3138 dobj edema,had
R1766 T3149 T3150 punct (,g
R1767 T3150 T3138 parataxis g,had
R1768 T3151 T3150 nmod Figure,g
R1769 T3152 T3150 nmod 1f,g
R1770 T3153 T3150 punct ",",g
R1771 T3154 T3150 punct ),g
R1772 T3155 T3138 punct .,had
R1773 T3157 T3158 mark Because,were
R1774 T3158 T3160 advcl were,investigated
R1775 T3159 T3158 nsubj we,were
R1776 T3161 T3158 acomp able,were
R1777 T3162 T3163 aux to,recover
R1778 T3163 T3161 xcomp recover,able
R1779 T3164 T3165 nmod Ptdsr,embryos
R1780 T3165 T3163 dobj embryos,recover
R1781 T3166 T3164 punct -,Ptdsr
R1782 T3167 T3164 punct /,Ptdsr
R1783 T3168 T3164 punct -,Ptdsr
R1784 T3169 T3163 prep until,recover
R1785 T3170 T3169 pobj E18.5,until
R1786 T3171 T3160 punct ", ",investigated
R1787 T3172 T3160 nsubj we,investigated
R1788 T3173 T3174 mark whether,born
R1789 T3174 T3160 ccomp born,investigated
R1790 T3175 T3176 compound Ptdsr,knockout
R1791 T3176 T3178 compound knockout,mice
R1792 T3177 T3176 punct -,knockout
R1793 T3178 T3174 nsubjpass mice,born
R1794 T3179 T3174 aux could,born
R1795 T3180 T3174 auxpass be,born
R1796 T3181 T3174 advcl alive,born
R1797 T3182 T3160 punct .,investigated
R1798 T3184 T3185 amod Careful,observation
R1799 T3185 T3186 nsubj observation,allowed
R1800 T3187 T3185 prep of,observation
R1801 T3188 T3189 amod timed,matings
R1802 T3189 T3187 pobj matings,of
R1803 T3190 T3191 nsubj us,recover
R1804 T3191 T3186 ccomp recover,allowed
R1805 T3192 T3191 aux to,recover
R1806 T3193 T3194 nmod Ptdsr,neonates
R1807 T3194 T3191 dobj neonates,recover
R1808 T3195 T3193 punct -,Ptdsr
R1809 T3196 T3193 punct /,Ptdsr
R1810 T3197 T3193 punct -,Ptdsr
R1811 T3198 T3186 punct ", ",allowed
R1812 T3199 T3186 cc but,allowed
R1813 T3200 T3201 amod homozygous,pups
R1814 T3201 T3202 nsubj pups,died
R1815 T3202 T3186 conj died,allowed
R1816 T3203 T3202 prep during,died
R1817 T3204 T3203 pobj delivery,during
R1818 T3205 T3203 cc or,during
R1819 T3206 T3203 conj within,during
R1820 T3207 T3206 pobj minutes,within
R1821 T3208 T3207 prep after,minutes
R1822 T3209 T3208 pobj birth,after
R1823 T3210 T3202 punct .,died
R1824 T3212 T3213 npadvmod Ptdsr,deficient
R1825 T3213 T3215 amod deficient,neonates
R1826 T3214 T3213 punct -,deficient
R1827 T3215 T3216 nsubj neonates,were
R1828 T3217 T3216 advmod also,were
R1829 T3218 T3219 npadvmod growth,retarded
R1830 T3219 T3216 acomp retarded,were
R1831 T3220 T3219 punct -,retarded
R1832 T3221 T3216 punct ", ",were
R1833 T3222 T3216 conj had,were
R1834 T3223 T3224 det a,appearance
R1835 T3224 T3222 dobj appearance,had
R1836 T3225 T3224 amod pale,appearance
R1837 T3226 T3222 cc and,had
R1838 T3227 T3222 conj displayed,had
R1839 T3228 T3229 amod various,malformations
R1840 T3229 T3227 dobj malformations,displayed
R1841 T3230 T3216 punct .,were
R1842 T3232 T3233 nsubj These,included
R1843 T3234 T3235 amod cleft,palate
R1844 T3235 T3233 dobj palate,included
R1845 T3236 T3235 punct ", ",palate
R1846 T3237 T3238 amod abnormal,shape
R1847 T3238 T3235 conj shape,palate
R1848 T3239 T3238 compound head,shape
R1849 T3240 T3238 punct ", ",shape
R1850 T3241 T3238 conj absence,shape
R1851 T3242 T3241 prep of,absence
R1852 T3243 T3242 pobj eyes,of
R1853 T3244 T3241 cc and,absence
R1854 T3245 T3246 amod edematous,skin
R1855 T3246 T3241 conj skin,absence
R1856 T3247 T3248 punct (,1h
R1857 T3248 T3233 parataxis 1h,included
R1858 T3249 T3248 compound Figure,1h
R1859 T3250 T3248 punct ),1h
R1860 T3251 T3233 punct .,included
R1861 T3253 T3254 advmod Thus,resulted
R1862 T3255 T3254 punct ", ",resulted
R1863 T3256 T3254 nsubj deletion,resulted
R1864 T3257 T3256 prep of,deletion
R1865 T3258 T3259 det the,gene
R1866 T3259 T3257 pobj gene,of
R1867 T3260 T3259 compound Ptdsr,gene
R1868 T3261 T3254 prep in,resulted
R1869 T3262 T3263 amod perinatal,lethality
R1870 T3263 T3261 pobj lethality,in
R1871 T3264 T3254 prep with,resulted
R1872 T3265 T3266 amod variable,severity
R1873 T3266 T3264 pobj severity,with
R1874 T3267 T3266 cc and,severity
R1875 T3268 T3266 conj penetrance,severity
R1876 T3269 T3266 prep of,severity
R1877 T3270 T3269 pobj phenotypes,of
R1878 T3271 T3254 punct .,resulted
R1885 T3609 T3608 prep of,Expression
R1886 T3610 T3609 pobj Ptdsr,of
R1887 T3611 T3608 prep during,Expression
R1888 T3612 T3611 pobj embryogenesis,during
R1889 T3613 T3611 cc and,during
R1890 T3614 T3611 conj in,during
R1891 T3615 T3616 amod adult,tissues
R1892 T3616 T3614 pobj tissues,in
R1893 T3618 T3619 det The,lethality
R1894 T3619 T3622 nsubj lethality,indicates
R1895 T3620 T3619 amod observed,lethality
R1896 T3621 T3619 amod perinatal,lethality
R1897 T3623 T3624 mark that,plays
R1898 T3624 T3622 ccomp plays,indicates
R1899 T3625 T3624 nsubj Ptdsr,plays
R1900 T3626 T3627 det an,role
R1901 T3627 T3624 dobj role,plays
R1902 T3628 T3627 amod important,role
R1903 T3629 T3624 prep during,plays
R1904 T3630 T3629 pobj development,during
R1905 T3631 T3622 punct .,indicates
R1906 T3633 T3634 nsubj Analysis,showed
R1907 T3635 T3633 prep by,Analysis
R1908 T3636 T3637 compound RT,PCR
R1909 T3637 T3635 pobj PCR,by
R1910 T3638 T3637 punct -,PCR
R1911 T3639 T3640 punct (,shown
R1912 T3640 T3637 parataxis shown,PCR
R1913 T3641 T3640 nsubj data,shown
R1914 T3642 T3640 neg not,shown
R1915 T3643 T3640 punct ),shown
R1916 T3644 T3645 mark that,expressed
R1917 T3645 T3634 ccomp expressed,showed
R1918 T3646 T3645 nsubjpass Ptdsr,expressed
R1919 T3647 T3645 auxpass is,expressed
R1920 T3648 T3649 advmod early,in
R1921 T3649 T3645 prep in,expressed
R1922 T3650 T3649 pobj development,in
R1923 T3651 T3634 punct ", ",showed
R1924 T3652 T3653 mark because,were
R1925 T3653 T3634 advcl were,showed
R1926 T3654 T3653 nsubj we,were
R1927 T3655 T3653 acomp able,were
R1928 T3656 T3657 aux to,detect
R1929 T3657 T3655 xcomp detect,able
R1930 T3658 T3659 compound Ptdsr,transcripts
R1931 T3659 T3657 dobj transcripts,detect
R1932 T3660 T3657 prep in,detect
R1933 T3661 T3662 compound ES,cells
R1934 T3662 T3660 pobj cells,in
R1935 T3663 T3662 cc and,cells
R1936 T3664 T3662 conj embryos,cells
R1937 T3665 T3657 prep at,detect
R1938 T3666 T3667 det all,stages
R1939 T3667 T3665 pobj stages,at
R1940 T3668 T3667 amod developmental,stages
R1941 T3669 T3634 punct .,showed
R1942 T3671 T3672 aux To,analyze
R1943 T3672 T3673 advcl analyze,made
R1944 T3674 T3672 prep in,analyze
R1945 T3675 T3676 amod more,detail
R1946 T3676 T3674 pobj detail,in
R1947 T3677 T3678 det the,patterns
R1948 T3678 T3672 dobj patterns,analyze
R1949 T3679 T3678 amod temporal,patterns
R1950 T3680 T3679 cc and,temporal
R1951 T3681 T3679 conj spatial,temporal
R1952 T3682 T3678 compound expression,patterns
R1953 T3683 T3678 prep of,patterns
R1954 T3684 T3683 pobj Ptdsr,of
R1955 T3685 T3672 punct ", ",analyze
R1956 T3686 T3672 cc and,analyze
R1957 T3687 T3688 aux to,correlate
R1958 T3688 T3672 conj correlate,analyze
R1959 T3689 T3690 compound expression,patterns
R1960 T3690 T3688 dobj patterns,correlate
R1961 T3691 T3688 prep with,correlate
R1962 T3692 T3693 amod observed,malformations
R1963 T3693 T3691 pobj malformations,with
R1964 T3694 T3693 amod pathological,malformations
R1965 T3695 T3673 punct ", ",made
R1966 T3696 T3673 nsubj we,made
R1967 T3697 T3673 dobj use,made
R1968 T3698 T3673 prep of,made
R1969 T3699 T3700 det a,line
R1970 T3700 T3698 pobj line,of
R1971 T3701 T3702 compound Ptdsr,geo
R1972 T3702 T3700 compound geo,line
R1973 T3703 T3702 punct -,geo
R1974 T3704 T3702 compound β,geo
R1975 T3705 T3702 punct -,geo
R1976 T3706 T3707 compound gene,trap
R1977 T3707 T3700 compound trap,line
R1978 T3708 T3707 punct -,trap
R1979 T3709 T3700 compound reporter,line
R1980 T3710 T3700 compound mouse,line
R1981 T3711 T3700 acl generated,line
R1982 T3712 T3711 prep from,generated
R1983 T3713 T3714 det a,clone
R1984 T3714 T3712 pobj clone,from
R1985 T3715 T3714 compound Ptdsr,clone
R1986 T3716 T3717 compound gene,trap
R1987 T3717 T3714 compound trap,clone
R1988 T3718 T3717 punct -,trap
R1989 T3719 T3720 compound ES,cell
R1990 T3720 T3714 compound cell,clone
R1991 T3721 T3673 punct .,made
R1992 T3723 T3724 det This,line
R1993 T3724 T3725 nsubj line,has
R1994 T3726 T3727 det an,insertion
R1995 T3727 T3725 dobj insertion,has
R1996 T3728 T3727 prep of,insertion
R1997 T3729 T3730 compound β,galactosidase
R1998 T3730 T3728 pobj galactosidase,of
R1999 T3731 T3730 punct -,galactosidase
R2000 T3732 T3725 prep in,has
R2001 T3733 T3734 det the,region
R2002 T3734 T3732 pobj region,in
R2003 T3735 T3734 nummod 3,region
R2004 T3736 T3735 punct ',3
R2005 T3737 T3734 prep of,region
R2006 T3738 T3739 det the,gene
R2007 T3739 T3737 pobj gene,of
R2008 T3740 T3741 punct (,2a
R2009 T3741 T3725 parataxis 2a,has
R2010 T3742 T3741 compound Figure,2a
R2011 T3743 T3741 punct ),2a
R2012 T3744 T3725 punct .,has
R2013 T3746 T3747 nsubj We,examined
R2014 T3748 T3747 advmod first,examined
R2015 T3749 T3750 compound Ptdsr,expression
R2016 T3750 T3747 dobj expression,examined
R2017 T3751 T3747 prep by,examined
R2018 T3752 T3753 compound X,Gal
R2019 T3753 T3755 compound Gal,staining
R2020 T3754 T3753 punct -,Gal
R2021 T3755 T3751 pobj staining,by
R2022 T3756 T3747 prep in,examined
R2023 T3757 T3758 amod heterozygous,embryos
R2024 T3758 T3756 pobj embryos,in
R2025 T3759 T3758 acl staged,embryos
R2026 T3760 T3759 prep from,staged
R2027 T3761 T3760 pobj E9.5,from
R2028 T3762 T3760 prep to,from
R2029 T3763 T3762 pobj E12.5,to
R2030 T3764 T3747 punct .,examined
R2031 T3766 T3767 det These,stages
R2032 T3767 T3769 nsubjpass stages,chosen
R2033 T3768 T3767 amod developmental,stages
R2034 T3770 T3769 auxpass were,chosen
R2035 T3771 T3772 advmod so,investigate
R2036 T3772 T3769 advcl investigate,chosen
R2037 T3773 T3772 mark as,investigate
R2038 T3774 T3772 aux to,investigate
R2039 T3775 T3776 compound Ptdsr,expression
R2040 T3776 T3772 dobj expression,investigate
R2041 T3777 T3772 prep in,investigate
R2042 T3778 T3779 amod affected,organs
R2043 T3779 T3777 pobj organs,in
R2044 T3780 T3781 amod prior,to
R2045 T3781 T3772 prep to,investigate
R2046 T3782 T3783 det the,onset
R2047 T3783 T3781 pobj onset,to
R2048 T3784 T3783 prep of,onset
R2049 T3785 T3786 amod pathological,malformations
R2050 T3786 T3784 pobj malformations,of
R2051 T3787 T3783 prep in,onset
R2052 T3788 T3789 nmod Ptdsr,embryos
R2053 T3789 T3787 pobj embryos,in
R2054 T3790 T3788 punct -,Ptdsr
R2055 T3791 T3788 punct /,Ptdsr
R2056 T3792 T3788 punct -,Ptdsr
R2057 T3793 T3769 punct .,chosen
R2058 T3795 T3796 prep At,found
R2059 T3797 T3795 pobj E9.5,At
R2060 T3798 T3796 nsubj we,found
R2061 T3799 T3800 compound Ptdsr,expression
R2062 T3800 T3796 dobj expression,found
R2063 T3801 T3796 prep in,found
R2064 T3802 T3803 det the,tube
R2065 T3803 T3801 pobj tube,in
R2066 T3804 T3803 amod developing,tube
R2067 T3805 T3803 amod neural,tube
R2068 T3806 T3803 punct ", ",tube
R2069 T3807 T3803 conj somites,tube
R2070 T3808 T3807 punct ", ",somites
R2071 T3809 T3807 conj heart,somites
R2072 T3810 T3809 punct ", ",heart
R2073 T3811 T3809 conj gut,heart
R2074 T3812 T3811 cc and,gut
R2075 T3813 T3814 amod branchial,arches
R2076 T3814 T3811 conj arches,gut
R2077 T3815 T3816 punct (,2b
R2078 T3816 T3796 parataxis 2b,found
R2079 T3817 T3816 compound Figure,2b
R2080 T3818 T3816 punct ),2b
R2081 T3819 T3796 punct .,found
R2082 T3821 T3822 prep At,remained
R2083 T3823 T3821 pobj E10.5,At
R2084 T3824 T3822 punct ", ",remained
R2085 T3825 T3826 compound Ptdsr,expression
R2086 T3826 T3822 nsubj expression,remained
R2087 T3827 T3822 acomp high,remained
R2088 T3828 T3822 prep in,remained
R2089 T3829 T3830 det the,system
R2090 T3830 T3828 pobj system,in
R2091 T3831 T3830 amod developing,system
R2092 T3832 T3830 amod nervous,system
R2093 T3833 T3822 punct ", ",remained
R2094 T3834 T3822 prep with,remained
R2095 T3835 T3836 advmod most,staining
R2096 T3836 T3834 pobj staining,with
R2097 T3837 T3836 amod intense,staining
R2098 T3838 T3836 prep in,staining
R2099 T3839 T3840 det the,forebrain
R2100 T3840 T3838 pobj forebrain,in
R2101 T3841 T3840 punct ", ",forebrain
R2102 T3842 T3840 conj hindbrain,forebrain
R2103 T3843 T3842 cc and,hindbrain
R2104 T3844 T3845 amod neural,tube
R2105 T3845 T3842 conj tube,hindbrain
R2106 T3846 T3822 punct .,remained
R2107 T3848 T3849 prep At,detected
R2108 T3850 T3851 det this,stage
R2109 T3851 T3848 pobj stage,At
R2110 T3852 T3851 prep of,stage
R2111 T3853 T3852 pobj embryogenesis,of
R2112 T3854 T3849 punct ", ",detected
R2113 T3855 T3856 amod high,levels
R2114 T3856 T3849 nsubjpass levels,detected
R2115 T3857 T3856 prep of,levels
R2116 T3858 T3859 compound Ptdsr,expression
R2117 T3859 T3857 pobj expression,of
R2118 T3860 T3849 aux could,detected
R2119 T3861 T3849 advmod also,detected
R2120 T3862 T3849 auxpass be,detected
R2121 T3863 T3849 prep in,detected
R2122 T3864 T3865 det the,buds
R2123 T3865 T3863 pobj buds,in
R2124 T3866 T3865 amod developing,buds
R2125 T3867 T3865 compound limb,buds
R2126 T3868 T3865 cc and,buds
R2127 T3869 T3865 conj eyes,buds
R2128 T3870 T3871 punct (,2b
R2129 T3871 T3849 parataxis 2b,detected
R2130 T3872 T3871 compound Figure,2b
R2131 T3873 T3871 punct ),2b
R2132 T3874 T3849 punct .,detected
R2133 T3876 T3877 compound Ptdsr,expression
R2134 T3877 T3878 nsubjpass expression,altered
R2135 T3879 T3878 auxpass was,altered
R2136 T3880 T3878 prep at,altered
R2137 T3881 T3880 pobj E12.5,at
R2138 T3882 T3878 punct ", ",altered
R2139 T3883 T3878 prep with,altered
R2140 T3884 T3885 amod most,staining
R2141 T3885 T3883 pobj staining,with
R2142 T3886 T3885 amod intensive,staining
R2143 T3887 T3888 compound β,galactosidase
R2144 T3888 T3885 compound galactosidase,staining
R2145 T3889 T3888 punct -,galactosidase
R2146 T3890 T3885 prep in,staining
R2147 T3891 T3892 det the,eyes
R2148 T3892 T3890 pobj eyes,in
R2149 T3893 T3892 punct ", ",eyes
R2150 T3894 T3895 amod developing,condensations
R2151 T3895 T3892 conj condensations,eyes
R2152 T3896 T3895 prep of,condensations
R2153 T3897 T3898 det the,buds
R2154 T3898 T3896 pobj buds,of
R2155 T3899 T3898 compound limb,buds
R2156 T3900 T3895 punct ", ",condensations
R2157 T3901 T3902 amod neural,tube
R2158 T3902 T3895 conj tube,condensations
R2159 T3903 T3902 cc and,tube
R2160 T3904 T3902 conj brain,tube
R2161 T3905 T3906 punct (,2b
R2162 T3906 T3878 parataxis 2b,altered
R2163 T3907 T3906 compound Figure,2b
R2164 T3908 T3906 punct ),2b
R2165 T3909 T3878 punct .,altered
R2166 T3911 T3912 amod Transverse,sections
R2167 T3912 T3913 nsubj sections,showed
R2168 T3914 T3912 prep of,sections
R2169 T3915 T3916 compound X,Gal
R2170 T3916 T3918 npadvmod Gal,stained
R2171 T3917 T3916 punct -,Gal
R2172 T3918 T3920 amod stained,embryos
R2173 T3919 T3918 punct -,stained
R2174 T3920 T3914 pobj embryos,of
R2175 T3921 T3912 prep at,sections
R2176 T3922 T3921 pobj E12.5,at
R2177 T3923 T3924 det an,pattern
R2178 T3924 T3913 dobj pattern,showed
R2179 T3925 T3924 amod asymmetric,pattern
R2180 T3926 T3924 compound expression,pattern
R2181 T3927 T3924 prep in,pattern
R2182 T3928 T3929 det the,tube
R2183 T3929 T3927 pobj tube,in
R2184 T3930 T3929 amod neural,tube
R2185 T3931 T3924 prep with,pattern
R2186 T3932 T3933 amod intense,staining
R2187 T3933 T3931 pobj staining,with
R2188 T3934 T3933 prep of,staining
R2189 T3935 T3936 det the,layer
R2190 T3936 T3934 pobj layer,of
R2191 T3937 T3936 amod central,layer
R2192 T3938 T3936 compound mantle,layer
R2193 T3939 T3924 cc but,pattern
R2194 T3940 T3941 det no,expression
R2195 T3941 T3924 conj expression,pattern
R2196 T3942 T3941 prep in,expression
R2197 T3943 T3944 det the,part
R2198 T3944 T3942 pobj part,in
R2199 T3945 T3944 amod dorsal,part
R2200 T3946 T3944 prep of,part
R2201 T3947 T3948 det the,tube
R2202 T3948 T3946 pobj tube,of
R2203 T3949 T3948 amod neural,tube
R2204 T3950 T3951 punct (,2c
R2205 T3951 T3944 parataxis 2c,part
R2206 T3952 T3951 prep for,2c
R2207 T3953 T3952 pobj example,for
R2208 T3954 T3951 punct ", ",2c
R2209 T3955 T3956 det the,plate
R2210 T3956 T3951 dep plate,2c
R2211 T3957 T3956 compound roof,plate
R2212 T3958 T3951 punct ;,2c
R2213 T3959 T3951 compound Figure,2c
R2214 T3960 T3951 punct ),2c
R2215 T3961 T3913 punct .,showed
R2216 T3963 T3964 nsubjpass Expression,observed
R2217 T3964 T3979 ccomp observed,expressed
R2218 T3965 T3963 prep in,Expression
R2219 T3966 T3967 amod dorsal,ganglia
R2220 T3967 T3965 pobj ganglia,in
R2221 T3968 T3967 compound root,ganglia
R2222 T3969 T3967 amod lateral,ganglia
R2223 T3970 T3969 prep to,lateral
R2224 T3971 T3972 det the,tube
R2225 T3972 T3970 pobj tube,to
R2226 T3973 T3972 amod neural,tube
R2227 T3974 T3969 cc and,lateral
R2228 T3975 T3969 conj in,lateral
R2229 T3976 T3977 det the,somites
R2230 T3977 T3975 pobj somites,in
R2231 T3978 T3964 auxpass was,observed
R2232 T3980 T3979 punct ;,expressed
R2233 T3981 T3979 nsubjpass Ptdsr,expressed
R2234 T3982 T3979 auxpass was,expressed
R2235 T3983 T3979 prep throughout,expressed
R2236 T3984 T3985 det the,structure
R2237 T3985 T3983 pobj structure,throughout
R2238 T3986 T3985 compound somite,structure
R2239 T3987 T3988 punct (,2d
R2240 T3988 T3985 parataxis 2d,structure
R2241 T3989 T3988 dep myotome,2d
R2242 T3990 T3989 punct ", ",myotome
R2243 T3991 T3989 conj dermatome,myotome
R2244 T3992 T3991 cc and,dermatome
R2245 T3993 T3991 conj sclerotome,dermatome
R2246 T3994 T3988 punct ;,2d
R2247 T3995 T3988 compound Figure,2d
R2248 T3996 T3988 punct ),2d
R2249 T3997 T3979 punct .,expressed
R2250 T3999 T4000 compound Expression,boundaries
R2251 T4000 T4001 nsubj boundaries,were
R2252 T4002 T4000 prep between,boundaries
R2253 T4003 T4002 pobj somites,between
R2254 T4004 T4001 acomp evident,were
R2255 T4005 T4001 punct ", ",were
R2256 T4006 T4001 prep with,were
R2257 T4007 T4008 det no,expression
R2258 T4008 T4006 pobj expression,with
R2259 T4009 T4008 prep in,expression
R2260 T4010 T4011 det the,interzones
R2261 T4011 T4009 pobj interzones,in
R2262 T4012 T4011 amod segmental,interzones
R2263 T4013 T4011 punct ", ",interzones
R2264 T4014 T4015 dep which,correspond
R2265 T4015 T4011 relcl correspond,interzones
R2266 T4016 T4015 prep to,correspond
R2267 T4017 T4018 det the,discs
R2268 T4018 T4016 pobj discs,to
R2269 T4019 T4018 amod prospective,discs
R2270 T4020 T4018 amod intervertebral,discs
R2271 T4021 T4022 punct (,2d
R2272 T4022 T4001 parataxis 2d,were
R2273 T4023 T4022 compound Figure,2d
R2274 T4024 T4022 punct ),2d
R2275 T4025 T4001 punct .,were
R2276 T4027 T4028 amod Transverse,sections
R2277 T4028 T4029 nsubj sections,revealed
R2278 T4030 T4028 prep of,sections
R2279 T4031 T4032 det the,eye
R2280 T4032 T4030 pobj eye,of
R2281 T4033 T4032 amod developing,eye
R2282 T4034 T4028 prep at,sections
R2283 T4035 T4034 pobj E12.5,at
R2284 T4036 T4037 amod strong,expression
R2285 T4037 T4029 dobj expression,revealed
R2286 T4038 T4037 compound Ptdsr,expression
R2287 T4039 T4029 prep in,revealed
R2288 T4040 T4041 det the,layer
R2289 T4041 T4039 pobj layer,in
R2290 T4042 T4041 amod inner,layer
R2291 T4043 T4041 prep of,layer
R2292 T4044 T4045 det the,cup
R2293 T4045 T4043 pobj cup,of
R2294 T4046 T4045 amod neural,cup
R2295 T4047 T4045 punct ", ",cup
R2296 T4048 T4049 dep which,develop
R2297 T4049 T4045 relcl develop,cup
R2298 T4050 T4049 aux will,develop
R2299 T4051 T4049 advmod later,develop
R2300 T4052 T4049 prep into,develop
R2301 T4053 T4054 det the,retina
R2302 T4054 T4052 pobj retina,into
R2303 T4055 T4054 amod neural,retina
R2304 T4056 T4029 punct .,revealed
R2305 T4058 T4059 advmod Furthermore,detected
R2306 T4060 T4059 punct ", ",detected
R2307 T4061 T4062 compound Ptdsr,expression
R2308 T4062 T4059 nsubjpass expression,detected
R2309 T4063 T4059 auxpass was,detected
R2310 T4064 T4059 prep in,detected
R2311 T4065 T4066 det the,cells
R2312 T4066 T4064 pobj cells,in
R2313 T4067 T4068 amod primary,lens
R2314 T4068 T4066 compound lens,cells
R2315 T4069 T4066 compound fiber,cells
R2316 T4070 T4066 prep of,cells
R2317 T4071 T4072 det the,lens
R2318 T4072 T4070 pobj lens,of
R2319 T4073 T4072 amod developing,lens
R2320 T4074 T4075 punct (,2e
R2321 T4075 T4059 parataxis 2e,detected
R2322 T4076 T4075 compound Figure,2e
R2323 T4077 T4075 punct ),2e
R2324 T4078 T4059 punct .,detected
R2325 T4080 T4081 nsubj We,investigated
R2326 T4082 T4081 advmod carefully,investigated
R2327 T4083 T4084 mark whether,expressed
R2328 T4084 T4081 ccomp expressed,investigated
R2329 T4085 T4084 nsubjpass Ptdsr,expressed
R2330 T4086 T4084 auxpass is,expressed
R2331 T4087 T4084 prep from,expressed
R2332 T4088 T4087 pobj E10.5,from
R2333 T4089 T4087 prep to,from
R2334 T4090 T4089 pobj E12.5,to
R2335 T4091 T4084 prep in,expressed
R2336 T4092 T4093 det the,kidney
R2337 T4093 T4091 pobj kidney,in
R2338 T4094 T4093 amod developing,kidney
R2339 T4095 T4093 cc and,kidney
R2340 T4096 T4093 conj lungs,kidney
R2341 T4097 T4081 punct ", ",investigated
R2342 T4098 T4081 cc but,investigated
R2343 T4099 T4100 det no,expression
R2344 T4100 T4101 nsubjpass expression,detected
R2345 T4101 T4081 conj detected,investigated
R2346 T4102 T4101 aux could,detected
R2347 T4103 T4101 auxpass be,detected
R2348 T4104 T4101 advcl indicating,detected
R2349 T4105 T4106 mark that,required
R2350 T4106 T4104 ccomp required,indicating
R2351 T4107 T4108 compound Ptdsr,expression
R2352 T4108 T4106 nsubjpass expression,required
R2353 T4109 T4106 auxpass is,required
R2354 T4110 T4106 advmod only,required
R2355 T4111 T4106 prep at,required
R2356 T4112 T4113 amod later,stages
R2357 T4113 T4111 pobj stages,at
R2358 T4114 T4113 prep in,stages
R2359 T4115 T4116 det the,development
R2360 T4116 T4114 pobj development,in
R2361 T4117 T4116 prep of,development
R2362 T4118 T4119 det these,organs
R2363 T4119 T4117 pobj organs,of
R2364 T4120 T4121 punct (,see
R2365 T4121 T4101 parataxis see,detected
R2366 T4122 T4121 advmod below,see
R2367 T4123 T4121 punct ),see
R2368 T4124 T4101 punct .,detected
R2369 T4126 T4127 nsubj Hybridization,revealed
R2370 T4128 T4126 prep of,Hybridization
R2371 T4129 T4130 det a,blot
R2372 T4130 T4128 pobj blot,of
R2373 T4131 T4132 amod multiple,tissue
R2374 T4132 T4130 compound tissue,blot
R2375 T4133 T4132 punct -,tissue
R2376 T4134 T4130 compound northern,blot
R2377 T4135 T4136 det a,transcript
R2378 T4136 T4127 dobj transcript,revealed
R2379 T4137 T4136 amod single,transcript
R2380 T4138 T4136 prep of,transcript
R2381 T4139 T4140 quantmod about,1.8
R2382 T4140 T4141 nummod 1.8,kb
R2383 T4141 T4138 pobj kb,of
R2384 T4142 T4127 prep in,revealed
R2385 T4143 T4144 advmod almost,tissue
R2386 T4144 T4142 pobj tissue,in
R2387 T4145 T4144 det every,tissue
R2388 T4146 T4144 acl analyzed,tissue
R2389 T4147 T4146 prep in,analyzed
R2390 T4148 T4149 amod adult,mice
R2391 T4149 T4147 pobj mice,in
R2392 T4150 T4151 punct (,2f
R2393 T4151 T4127 parataxis 2f,revealed
R2394 T4152 T4151 compound Figure,2f
R2395 T4153 T4151 punct ),2f
R2396 T4154 T4127 punct .,revealed
R2397 T4156 T4157 det The,expression
R2398 T4157 T4160 nsubjpass expression,observed
R2399 T4158 T4159 advmod most,prominent
R2400 T4159 T4157 amod prominent,expression
R2401 T4161 T4160 auxpass was,observed
R2402 T4162 T4160 prep in,observed
R2403 T4163 T4162 pobj testis,in
R2404 T4164 T4163 punct ", ",testis
R2405 T4165 T4163 conj thymus,testis
R2406 T4166 T4165 punct ", ",thymus
R2407 T4167 T4165 conj kidney,thymus
R2408 T4168 T4167 punct ", ",kidney
R2409 T4169 T4167 conj liver,kidney
R2410 T4170 T4169 cc and,liver
R2411 T4171 T4169 conj skin,liver
R2412 T4172 T4160 punct ", ",observed
R2413 T4173 T4160 prep with,observed
R2414 T4174 T4175 amod moderate,expression
R2415 T4175 T4173 pobj expression,with
R2416 T4176 T4174 prep to,moderate
R2417 T4177 T4176 amod low,to
R2418 T4178 T4175 prep in,expression
R2419 T4179 T4178 pobj lung,in
R2420 T4180 T4179 punct ", ",lung
R2421 T4181 T4182 amod small,intestine
R2422 T4182 T4179 conj intestine,lung
R2423 T4183 T4182 punct ", ",intestine
R2424 T4184 T4182 conj spleen,intestine
R2425 T4185 T4184 punct ", ",spleen
R2426 T4186 T4184 conj stomach,spleen
R2427 T4187 T4186 cc and,stomach
R2428 T4188 T4189 amod skeletal,muscle
R2429 T4189 T4186 conj muscle,stomach
R2430 T4190 T4160 punct .,observed
R2431 T4192 T4193 advmod Thus,expressed
R2432 T4194 T4193 punct ", ",expressed
R2433 T4195 T4193 nsubjpass Ptdsr,expressed
R2434 T4196 T4193 auxpass is,expressed
R2435 T4197 T4193 advmod ubiquitously,expressed
R2436 T4198 T4193 prep throughout,expressed
R2437 T4199 T4198 pobj embryogenesis,throughout
R2438 T4200 T4198 cc and,throughout
R2439 T4201 T4198 conj in,throughout
R2440 T4202 T4203 amod adult,tissues
R2441 T4203 T4201 pobj tissues,in
R2442 T4204 T4193 punct ", ",expressed
R2443 T4205 T4206 mark although,at
R2444 T4206 T4193 advcl at,expressed
R2445 T4207 T4208 amod different,levels
R2446 T4208 T4206 pobj levels,at
R2447 T4209 T4193 punct .,expressed
R2450 T4518 T4519 nsubjpass Ptdsr,required
R2451 T4520 T4519 auxpass is,required
R2452 T4521 T4519 prep for,required
R2453 T4522 T4523 amod normal,differentiation
R2454 T4523 T4521 pobj differentiation,for
R2455 T4524 T4523 nmod tissue,differentiation
R2456 T4525 T4524 cc and,tissue
R2457 T4526 T4524 conj organ,tissue
R2458 T4528 T4529 nsubj We,examined
R2459 T4530 T4529 advmod next,examined
R2460 T4531 T4532 det the,role
R2461 T4532 T4529 dobj role,examined
R2462 T4533 T4532 prep of,role
R2463 T4534 T4533 pobj Ptdsr,of
R2464 T4535 T4532 prep in,role
R2465 T4536 T4537 compound organ,development
R2466 T4537 T4535 pobj development,in
R2467 T4538 T4529 punct .,examined
R2468 T4540 T4541 amod Serial,sections
R2469 T4541 T4543 nsubjpass sections,taken
R2470 T4542 T4541 amod histological,sections
R2471 T4544 T4541 prep of,sections
R2472 T4545 T4546 nmod Ptdsr,embryos
R2473 T4546 T4544 pobj embryos,of
R2474 T4547 T4545 punct -,Ptdsr
R2475 T4548 T4545 punct /,Ptdsr
R2476 T4549 T4545 punct -,Ptdsr
R2477 T4550 T4545 cc and,Ptdsr
R2478 T4551 T4545 conj control,Ptdsr
R2479 T4552 T4543 auxpass were,taken
R2480 T4553 T4554 aux to,perform
R2481 T4554 T4543 advcl perform,taken
R2482 T4555 T4556 det a,analysis
R2483 T4556 T4554 dobj analysis,perform
R2484 T4557 T4556 amod detailed,analysis
R2485 T4558 T4556 amod morphological,analysis
R2486 T4559 T4556 prep of,analysis
R2487 T4560 T4561 det all,systems
R2488 T4561 T4559 pobj systems,of
R2489 T4562 T4561 compound organ,systems
R2490 T4563 T4554 prep during,perform
R2491 T4564 T4563 pobj development,during
R2492 T4565 T4543 punct .,taken
R2493 T4567 T4568 det A,delay
R2494 T4568 T4570 nsubjpass delay,observed
R2495 T4569 T4568 amod significant,delay
R2496 T4571 T4568 prep in,delay
R2497 T4572 T4573 nmod organ,differentiation
R2498 T4573 T4571 pobj differentiation,in
R2499 T4574 T4572 cc and,organ
R2500 T4575 T4572 conj tissue,organ
R2501 T4576 T4570 auxpass was,observed
R2502 T4577 T4570 prep at,observed
R2503 T4578 T4577 pobj E16.5,at
R2504 T4579 T4570 prep in,observed
R2505 T4580 T4579 pobj lungs,in
R2506 T4581 T4580 punct ", ",lungs
R2507 T4582 T4580 conj kidneys,lungs
R2508 T4583 T4582 cc and,kidneys
R2509 T4584 T4582 conj intestine,kidneys
R2510 T4585 T4570 punct .,observed
R2511 T4587 T4588 nsubj Lungs,were
R2512 T4589 T4587 prep of,Lungs
R2513 T4590 T4591 compound control,littermates
R2514 T4591 T4589 pobj littermates,of
R2515 T4592 T4593 advmod properly,developed
R2516 T4593 T4588 acomp developed,were
R2517 T4594 T4588 prep with,were
R2518 T4595 T4596 amod expanding,alveoli
R2519 T4596 T4594 pobj alveoli,with
R2520 T4597 T4598 punct (,3a
R2521 T4598 T4588 parataxis 3a,were
R2522 T4599 T4598 compound Figure,3a
R2523 T4600 T4598 punct ),3a
R2524 T4601 T4588 punct .,were
R2525 T4603 T4604 amod Terminal,bronchi
R2526 T4604 T4605 nsubj bronchi,were
R2527 T4606 T4604 cc and,bronchi
R2528 T4607 T4604 conj bronchioles,bronchi
R2529 T4608 T4605 advmod already,were
R2530 T4609 T4610 advmod well,developed
R2531 T4610 T4605 acomp developed,were
R2532 T4611 T4605 punct ", ",were
R2533 T4612 T4605 cc and,were
R2534 T4613 T4614 advmod terminally,differentiated
R2535 T4614 T4615 amod differentiated,cells
R2536 T4615 T4617 nsubj cells,were
R2537 T4616 T4615 amod epithelial,cells
R2538 T4617 T4605 conj were,were
R2539 T4618 T4615 prep with,cells
R2540 T4619 T4618 pobj cilia,with
R2541 T4620 T4619 prep on,cilia
R2542 T4621 T4622 det the,surface
R2543 T4622 T4620 pobj surface,on
R2544 T4623 T4622 amod luminal,surface
R2545 T4624 T4622 compound cell,surface
R2546 T4625 T4617 acomp present,were
R2547 T4626 T4617 punct .,were
R2548 T4628 T4629 prep In,were
R2549 T4630 T4628 pobj contrast,In
R2550 T4631 T4629 punct ", ",were
R2551 T4632 T4633 advmod almost,alveoli
R2552 T4633 T4629 nsubj alveoli,were
R2553 T4634 T4633 det no,alveoli
R2554 T4635 T4633 cc or,alveoli
R2555 T4636 T4633 conj bronchioles,alveoli
R2556 T4637 T4629 acomp present,were
R2557 T4638 T4629 prep in,were
R2558 T4639 T4640 nmod Ptdsr,lungs
R2559 T4640 T4638 pobj lungs,in
R2560 T4641 T4639 punct -,Ptdsr
R2561 T4642 T4639 punct /,Ptdsr
R2562 T4643 T4639 punct -,Ptdsr
R2563 T4644 T4629 punct ", ",were
R2564 T4645 T4629 advcl indicating,were
R2565 T4646 T4647 det a,delay
R2566 T4647 T4645 dobj delay,indicating
R2567 T4648 T4647 cc or,delay
R2568 T4649 T4647 conj arrest,delay
R2569 T4650 T4647 prep in,delay
R2570 T4651 T4652 compound lung,sacculation
R2571 T4652 T4650 pobj sacculation,in
R2572 T4653 T4652 cc and,sacculation
R2573 T4654 T4652 conj expansion,sacculation
R2574 T4655 T4629 punct .,were
R2575 T4657 T4658 advmod Instead,observed
R2576 T4659 T4658 punct ", ",observed
R2577 T4660 T4658 nsubj we,observed
R2578 T4661 T4662 det an,abundance
R2579 T4662 T4658 dobj abundance,observed
R2580 T4663 T4662 prep of,abundance
R2581 T4664 T4663 pobj mesenchyme,of
R2582 T4665 T4666 dep that,appeared
R2583 T4666 T4662 relcl appeared,abundance
R2584 T4667 T4668 advmod highly,immature
R2585 T4668 T4666 oprd immature,appeared
R2586 T4669 T4670 punct (,3g
R2587 T4670 T4658 parataxis 3g,observed
R2588 T4671 T4670 compound Figure,3g
R2589 T4672 T4670 punct ),3g
R2590 T4673 T4658 punct .,observed
R2591 T4675 T4676 det A,delay
R2592 T4676 T4678 nsubjpass delay,found
R2593 T4677 T4676 amod similar,delay
R2594 T4679 T4676 prep in,delay
R2595 T4680 T4681 compound tissue,differentiation
R2596 T4681 T4679 pobj differentiation,in
R2597 T4682 T4681 prep of,differentiation
R2598 T4683 T4684 nmod Ptdsr,embryos
R2599 T4684 T4682 pobj embryos,of
R2600 T4685 T4683 punct -,Ptdsr
R2601 T4686 T4683 punct /,Ptdsr
R2602 T4687 T4683 punct -,Ptdsr
R2603 T4688 T4678 auxpass was,found
R2604 T4689 T4678 prep in,found
R2605 T4690 T4691 det the,kidneys
R2606 T4691 T4689 pobj kidneys,in
R2607 T4692 T4693 punct (,3h
R2608 T4693 T4678 parataxis 3h,found
R2609 T4694 T4693 compound Figure,3h
R2610 T4695 T4693 punct ),3h
R2611 T4696 T4678 punct .,found
R2612 T4698 T4699 nsubj Kidneys,were
R2613 T4700 T4698 prep from,Kidneys
R2614 T4701 T4702 nmod Ptdsr,embryos
R2615 T4702 T4700 pobj embryos,from
R2616 T4703 T4701 punct +,Ptdsr
R2617 T4704 T4701 punct /,Ptdsr
R2618 T4705 T4701 punct +,Ptdsr
R2619 T4706 T4707 advmod well,developed
R2620 T4707 T4699 acomp developed,were
R2621 T4708 T4699 prep at,were
R2622 T4709 T4708 pobj E16.5,at
R2623 T4710 T4699 punct ", ",were
R2624 T4711 T4699 advcl showing,were
R2625 T4712 T4713 advmod terminally,differentiated
R2626 T4713 T4714 amod differentiated,glomeruli
R2627 T4714 T4711 dobj glomeruli,showing
R2628 T4715 T4714 prep with,glomeruli
R2629 T4716 T4717 poss Bowman,capsule
R2630 T4717 T4715 pobj capsule,with
R2631 T4718 T4716 case 's,Bowman
R2632 T4719 T4717 cc and,capsule
R2633 T4720 T4721 amod collecting,tubules
R2634 T4721 T4717 conj tubules,capsule
R2635 T4722 T4714 acl lined,glomeruli
R2636 T4723 T4722 prep with,lined
R2637 T4724 T4725 amod cuboidal,cells
R2638 T4725 T4723 pobj cells,with
R2639 T4726 T4725 amod epithelial,cells
R2640 T4727 T4728 punct (,3b
R2641 T4728 T4699 parataxis 3b,were
R2642 T4729 T4728 compound Figure,3b
R2643 T4730 T4728 punct ),3b
R2644 T4731 T4699 punct .,were
R2645 T4733 T4734 prep In,had
R2646 T4735 T4733 pobj contrast,In
R2647 T4736 T4734 punct ", ",had
R2648 T4737 T4738 npadvmod Ptdsr,deficient
R2649 T4738 T4740 amod deficient,kidneys
R2650 T4739 T4738 punct -,deficient
R2651 T4740 T4734 nsubj kidneys,had
R2652 T4741 T4742 advmod only,glomeruli
R2653 T4742 T4734 dobj glomeruli,had
R2654 T4743 T4742 amod primitive,glomeruli
R2655 T4744 T4734 prep at,had
R2656 T4745 T4744 pobj E16.5,at
R2657 T4746 T4734 punct ", ",had
R2658 T4747 T4734 cc and,had
R2659 T4748 T4749 amod collecting,tubules
R2660 T4749 T4750 nsubj tubules,were
R2661 T4750 T4734 conj were,had
R2662 T4751 T4752 advmod less,developed
R2663 T4752 T4750 acomp developed,were
R2664 T4753 T4752 advmod well,developed
R2665 T4754 T4752 punct -,developed
R2666 T4755 T4734 punct .,had
R2667 T4757 T4758 advmod Instead,was
R2668 T4759 T4758 punct ", ",was
R2669 T4760 T4761 det a,amount
R2670 T4761 T4758 nsubj amount,was
R2671 T4762 T4761 amod large,amount
R2672 T4763 T4761 prep of,amount
R2673 T4764 T4765 amod undifferentiated,mesenchyme
R2674 T4765 T4763 pobj mesenchyme,of
R2675 T4766 T4758 acomp present,was
R2676 T4767 T4758 prep in,was
R2677 T4768 T4769 nmod Ptdsr,kidneys
R2678 T4769 T4767 pobj kidneys,in
R2679 T4770 T4768 punct -,Ptdsr
R2680 T4771 T4768 punct /,Ptdsr
R2681 T4772 T4768 punct -,Ptdsr
R2682 T4773 T4774 punct (,3h
R2683 T4774 T4758 parataxis 3h,was
R2684 T4775 T4774 compound Figure,3h
R2685 T4776 T4774 punct ),3h
R2686 T4777 T4758 punct .,was
R2687 T4779 T4780 det A,delay
R2688 T4780 T4781 nsubjpass delay,found
R2689 T4782 T4780 prep in,delay
R2690 T4783 T4784 compound tissue,differentiation
R2691 T4784 T4782 pobj differentiation,in
R2692 T4785 T4781 auxpass was,found
R2693 T4786 T4781 advmod also,found
R2694 T4787 T4781 prep in,found
R2695 T4788 T4789 det the,intestine
R2696 T4789 T4787 pobj intestine,in
R2697 T4790 T4781 prep at,found
R2698 T4791 T4792 det this,stage
R2699 T4792 T4790 pobj stage,at
R2700 T4793 T4792 prep of,stage
R2701 T4794 T4793 pobj development,of
R2702 T4795 T4781 punct .,found
R2703 T4797 T4798 nmod Ptdsr,embryos
R2704 T4798 T4802 nsubj embryos,displayed
R2705 T4799 T4797 punct -,Ptdsr
R2706 T4800 T4797 punct /,Ptdsr
R2707 T4801 T4797 punct -,Ptdsr
R2708 T4803 T4804 advmod improperly,developed
R2709 T4804 T4805 amod developed,villi
R2710 T4805 T4802 dobj villi,displayed
R2711 T4806 T4805 cc and,villi
R2712 T4807 T4808 det an,submucosa
R2713 T4808 T4805 conj submucosa,villi
R2714 T4809 T4808 amod underdeveloped,submucosa
R2715 T4810 T4809 cc or,underdeveloped
R2716 T4811 T4809 conj absent,underdeveloped
R2717 T4812 T4813 punct (,3i
R2718 T4813 T4802 parataxis 3i,displayed
R2719 T4814 T4813 compound Figure,3i
R2720 T4815 T4813 punct ),3i
R2721 T4816 T4802 punct .,displayed
R2722 T4818 T4819 prep In,was
R2723 T4820 T4821 amod wild,type
R2724 T4821 T4823 compound type,embryos
R2725 T4822 T4821 punct -,type
R2726 T4823 T4818 pobj embryos,In
R2727 T4824 T4825 punct (,3c
R2728 T4825 T4819 parataxis 3c,was
R2729 T4826 T4825 compound Figure,3c
R2730 T4827 T4825 punct ),3c
R2731 T4828 T4819 punct ", ",was
R2732 T4829 T4830 amod intestinal,differentiation
R2733 T4830 T4819 nsubj differentiation,was
R2734 T4831 T4830 amod cellular,differentiation
R2735 T4832 T4819 advmod already,was
R2736 T4833 T4834 advmod highly,organized
R2737 T4834 T4819 acomp organized,was
R2738 T4835 T4819 punct ", ",was
R2739 T4836 T4819 prep with,was
R2740 T4837 T4838 amod intramural,cells
R2741 T4838 T4836 pobj cells,with
R2742 T4839 T4838 compound ganglion,cells
R2743 T4840 T4838 prep between,cells
R2744 T4841 T4842 det the,layers
R2745 T4842 T4840 pobj layers,between
R2746 T4843 T4842 amod external,layers
R2747 T4844 T4843 cc and,external
R2748 T4845 T4843 conj internal,external
R2749 T4846 T4842 amod muscular,layers
R2750 T4847 T4819 punct .,was
R2751 T4849 T4850 amod Such,cells
R2752 T4850 T4852 nsubj cells,were
R2753 T4851 T4850 amod neuronal,cells
R2754 T4853 T4852 acomp absent,were
R2755 T4854 T4853 prep from,absent
R2756 T4855 T4856 det the,intestine
R2757 T4856 T4854 pobj intestine,from
R2758 T4857 T4856 prep of,intestine
R2759 T4858 T4859 nmod Ptdsr,embryos
R2760 T4859 T4857 pobj embryos,of
R2761 T4860 T4858 punct -,Ptdsr
R2762 T4861 T4858 punct /,Ptdsr
R2763 T4862 T4858 punct -,Ptdsr
R2764 T4863 T4864 punct (,3i
R2765 T4864 T4852 parataxis 3i,were
R2766 T4865 T4864 compound Figure,3i
R2767 T4866 T4864 punct ),3i
R2768 T4867 T4852 punct ", ",were
R2769 T4868 T4852 advmod however,were
R2770 T4869 T4852 punct .,were
R2771 T4871 T4872 det Some,mice
R2772 T4872 T4877 nsubj mice,displayed
R2773 T4873 T4872 nmod Ptdsr,mice
R2774 T4874 T4873 punct -,Ptdsr
R2775 T4875 T4873 punct /,Ptdsr
R2776 T4876 T4873 punct -,Ptdsr
R2777 T4878 T4879 punct (,%
R2778 T4879 T4872 parataxis %,mice
R2779 T4880 T4879 nummod 4.5,%
R2780 T4881 T4879 punct ),%
R2781 T4882 T4877 advmod also,displayed
R2782 T4883 T4884 amod extensive,malformations
R2783 T4884 T4877 dobj malformations,displayed
R2784 T4885 T4884 compound brain,malformations
R2785 T4886 T4887 dep that,resulted
R2786 T4887 T4884 relcl resulted,malformations
R2787 T4888 T4887 prep in,resulted
R2788 T4889 T4890 advmod externally,visible
R2789 T4890 T4891 amod visible,abnormalities
R2790 T4891 T4888 pobj abnormalities,in
R2791 T4892 T4891 compound head,abnormalities
R2792 T4893 T4891 punct ", ",abnormalities
R2793 T4894 T4891 prep with,abnormalities
R2794 T4895 T4896 amod occasional,tissue
R2795 T4896 T4894 pobj tissue,with
R2796 T4897 T4896 amod ectopic,tissue
R2797 T4898 T4896 prep outside,tissue
R2798 T4899 T4900 det the,skull
R2799 T4900 T4898 pobj skull,outside
R2800 T4901 T4900 cc or,skull
R2801 T4902 T4900 conj exencephaly,skull
R2802 T4903 T4904 punct (,h
R2803 T4904 T4877 parataxis h,displayed
R2804 T4905 T4904 nmod Figure,h
R2805 T4906 T4904 nmod 1f,h
R2806 T4907 T4904 punct ",",h
R2807 T4908 T4904 punct ),h
R2808 T4909 T4877 punct .,displayed
R2809 T4911 T4912 amod Histological,analysis
R2810 T4912 T4913 nsubj analysis,revealed
R2811 T4914 T4915 det an,hyperplasia
R2812 T4915 T4913 dobj hyperplasia,revealed
R2813 T4916 T4915 amod extensive,hyperplasia
R2814 T4917 T4915 prep of,hyperplasia
R2815 T4918 T4919 compound brain,tissue
R2816 T4919 T4917 pobj tissue,of
R2817 T4920 T4913 prep with,revealed
R2818 T4921 T4920 pobj herniation,with
R2819 T4922 T4921 prep of,herniation
R2820 T4923 T4924 compound brain,tissue
R2821 T4924 T4922 pobj tissue,of
R2822 T4925 T4926 preconj either,through
R2823 T4926 T4921 prep through,herniation
R2824 T4927 T4928 det the,cap
R2825 T4928 T4926 pobj cap,through
R2826 T4929 T4928 compound skull,cap
R2827 T4930 T4928 punct -,cap
R2828 T4931 T4926 cc or,through
R2829 T4932 T4926 conj through,through
R2830 T4933 T4934 det the,skull
R2831 T4934 T4932 pobj skull,through
R2832 T4935 T4934 amod ventral,skull
R2833 T4936 T4937 punct (,j
R2834 T4937 T4913 parataxis j,revealed
R2835 T4938 T4937 nmod Figure,j
R2836 T4939 T4937 nmod 3d,j
R2837 T4940 T4937 punct ",",j
R2838 T4941 T4937 punct ),j
R2839 T4942 T4913 punct .,revealed
R2840 T4944 T4945 prep In,resulted
R2841 T4946 T4947 det the,cases
R2842 T4947 T4944 pobj cases,In
R2843 T4948 T4949 advmod most,severe
R2844 T4949 T4947 amod severe,cases
R2845 T4950 T4945 punct ", ",resulted
R2846 T4951 T4945 nsubj expansion,resulted
R2847 T4952 T4951 prep of,expansion
R2848 T4953 T4954 compound brain,tissue
R2849 T4954 T4952 pobj tissue,of
R2850 T4955 T4951 prep in,expansion
R2851 T4956 T4957 amod mutant,mice
R2852 T4957 T4955 pobj mice,in
R2853 T4958 T4945 prep in,resulted
R2854 T4959 T4960 amod further,perturbations
R2855 T4960 T4958 pobj perturbations,in
R2856 T4961 T4960 prep of,perturbations
R2857 T4962 T4963 amod cortical,structures
R2858 T4963 T4961 pobj structures,of
R2859 T4964 T4965 punct (,j
R2860 T4965 T4945 parataxis j,resulted
R2861 T4966 T4965 nmod Figure,j
R2862 T4967 T4965 nmod 3d,j
R2863 T4968 T4965 punct ",",j
R2864 T4969 T4965 punct ),j
R2865 T4970 T4945 punct .,resulted
R2866 T4972 T4973 prep Of,observed
R2867 T4974 T4972 pobj note,Of
R2868 T4975 T4973 punct ", ",observed
R2869 T4976 T4977 det a,phenotype
R2870 T4977 T4973 nsubjpass phenotype,observed
R2871 T4978 T4977 amod similar,phenotype
R2872 T4979 T4977 compound brain,phenotype
R2873 T4980 T4973 auxpass was,observed
R2874 T4981 T4973 prep in,observed
R2875 T4982 T4983 det the,line
R2876 T4983 T4981 pobj line,in
R2877 T4984 T4985 npadvmod Ptdsr,deficient
R2878 T4985 T4983 amod deficient,line
R2879 T4986 T4985 punct -,deficient
R2880 T4987 T4983 compound mouse,line
R2881 T4988 T4983 acl generated,line
R2882 T4989 T4988 agent by,generated
R2883 T4990 T4989 pobj Li,by
R2884 T4991 T4990 cc and,Li
R2885 T4992 T4990 conj colleagues,Li
R2886 T4993 T4994 punct [,31
R2887 T4994 T4973 parataxis 31,observed
R2888 T4995 T4994 punct ],31
R2889 T4996 T4973 punct .,observed
R2890 T4998 T4999 prep In,found
R2891 T5000 T4998 pobj contrast,In
R2892 T5001 T5000 prep to,contrast
R2893 T5002 T5003 det the,study
R2894 T5003 T5001 pobj study,to
R2895 T5004 T5003 prep of,study
R2896 T5005 T5004 pobj Li,of
R2897 T5006 T5007 advmod et,al.
R2898 T5007 T5005 advmod al.,Li
R2899 T5008 T5009 punct [,31
R2900 T5009 T5000 parataxis 31,contrast
R2901 T5010 T5009 punct ],31
R2902 T5011 T4999 punct ", ",found
R2903 T5012 T4999 advmod however,found
R2904 T5013 T4999 punct ", ",found
R2905 T5014 T4999 nsubj we,found
R2906 T5015 T5016 advmod almost,developed
R2907 T5016 T5018 amod developed,lungs
R2908 T5017 T5016 advmod normally,developed
R2909 T5018 T4999 dobj lungs,found
R2910 T5019 T4999 prep at,found
R2911 T5020 T5019 pobj birth,at
R2912 T5021 T4999 punct .,found
R2913 T5023 T5024 nmod Ptdsr,lungs
R2914 T5024 T5028 nsubj lungs,showed
R2915 T5025 T5023 punct -,Ptdsr
R2916 T5026 T5023 punct /,Ptdsr
R2917 T5027 T5023 punct -,Ptdsr
R2918 T5029 T5028 punct ", ",showed
R2919 T5030 T5028 prep in,showed
R2920 T5031 T5030 pobj comparison,in
R2921 T5032 T5031 prep to,comparison
R2922 T5033 T5034 amod wild,type
R2923 T5034 T5032 pobj type,to
R2924 T5035 T5034 punct -,type
R2925 T5036 T5028 punct ", ",showed
R2926 T5037 T5038 advmod only,delay
R2927 T5038 T5028 dobj delay,showed
R2928 T5039 T5038 det a,delay
R2929 T5040 T5038 amod slight,delay
R2930 T5041 T5038 prep in,delay
R2931 T5042 T5041 pobj maturation,in
R2932 T5043 T5028 cc and,showed
R2933 T5044 T5028 conj were,showed
R2934 T5045 T5046 advmod fully,ventilated
R2935 T5046 T5044 acomp ventilated,were
R2936 T5047 T5044 prep in,were
R2937 T5048 T5047 pobj neonates,in
R2938 T5049 T5044 prep in,were
R2939 T5050 T5051 amod most,cases
R2940 T5051 T5049 pobj cases,in
R2941 T5052 T5053 punct (,k
R2942 T5053 T5044 parataxis k,were
R2943 T5054 T5053 nmod Figure,k
R2944 T5055 T5053 nmod 3e,k
R2945 T5056 T5053 punct ",",k
R2946 T5057 T5053 punct ),k
R2947 T5058 T5028 punct .,showed
R2948 T5060 T5061 nsubj This,demonstrates
R2949 T5062 T5063 mark that,overcome
R2950 T5063 T5061 ccomp overcome,demonstrates
R2951 T5064 T5065 npadvmod Ptdsr,deficient
R2952 T5065 T5067 amod deficient,mice
R2953 T5066 T5065 punct -,deficient
R2954 T5067 T5063 nsubj mice,overcome
R2955 T5068 T5063 aux can,overcome
R2956 T5069 T5070 det the,delay
R2957 T5070 T5063 dobj delay,overcome
R2958 T5071 T5070 prep in,delay
R2959 T5072 T5073 amod embryonic,differentiation
R2960 T5073 T5071 pobj differentiation,in
R2961 T5074 T5073 compound lung,differentiation
R2962 T5075 T5063 cc and,overcome
R2963 T5076 T5063 conj display,overcome
R2964 T5077 T5078 amod normal,morphology
R2965 T5078 T5076 dobj morphology,display
R2966 T5079 T5078 compound lung,morphology
R2967 T5080 T5076 prep at,display
R2968 T5081 T5080 pobj birth,at
R2969 T5082 T5061 punct .,demonstrates
R2970 T5084 T5085 advmod Thus,appear
R2971 T5086 T5085 punct ", ",appear
R2972 T5087 T5085 nsubj it,appear
R2973 T5088 T5085 aux would,appear
R2974 T5089 T5090 advmod highly,unlikely
R2975 T5090 T5085 oprd unlikely,appear
R2976 T5091 T5092 mark that,die
R2977 T5092 T5085 ccomp die,appear
R2978 T5093 T5094 nmod Ptdsr,mice
R2979 T5094 T5092 nsubj mice,die
R2980 T5095 T5093 punct -,Ptdsr
R2981 T5096 T5093 punct /,Ptdsr
R2982 T5097 T5093 punct -,Ptdsr
R2983 T5098 T5092 prep from,die
R2984 T5099 T5100 amod respiratory,failure
R2985 T5100 T5098 pobj failure,from
R2986 T5101 T5085 punct .,appear
R2987 T5103 T5104 advcl Consistent,found
R2988 T5105 T5103 prep with,Consistent
R2989 T5106 T5107 det the,observations
R2990 T5107 T5105 pobj observations,with
R2991 T5108 T5107 prep of,observations
R2992 T5109 T5108 pobj Kunisaki,of
R2993 T5110 T5109 cc and,Kunisaki
R2994 T5111 T5109 conj colleagues,Kunisaki
R2995 T5112 T5113 punct [,32
R2996 T5113 T5103 parataxis 32,Consistent
R2997 T5114 T5113 punct ],32
R2998 T5115 T5104 punct ", ",found
R2999 T5116 T5104 nsubj we,found
R3000 T5117 T5118 advmod severely,blocked
R3001 T5118 T5119 amod blocked,differentiation
R3002 T5119 T5104 dobj differentiation,found
R3003 T5120 T5119 amod erythropoietic,differentiation
R3004 T5121 T5104 prep at,found
R3005 T5122 T5123 det an,stage
R3006 T5123 T5121 pobj stage,at
R3007 T5124 T5123 amod early,stage
R3008 T5125 T5123 compound erythroblast,stage
R3009 T5126 T5104 prep in,found
R3010 T5127 T5128 det the,liver
R3011 T5128 T5126 pobj liver,in
R3012 T5129 T5130 punct (,3l
R3013 T5130 T5104 parataxis 3l,found
R3014 T5131 T5130 nmod Figure,3l
R3015 T5132 T5130 nmod 3f,3l
R3016 T5133 T5130 punct ",",3l
R3017 T5134 T5130 punct ),3l
R3018 T5135 T5104 punct ", ",found
R3019 T5136 T5104 advcl suggesting,found
R3020 T5137 T5138 det an,explanation
R3021 T5138 T5136 dobj explanation,suggesting
R3022 T5139 T5138 prep for,explanation
R3023 T5140 T5141 det the,appearance
R3024 T5141 T5139 pobj appearance,for
R3025 T5142 T5143 advmod grossly,anemic
R3026 T5143 T5141 amod anemic,appearance
R3027 T5144 T5145 dep that,observed
R3028 T5145 T5141 relcl observed,appearance
R3029 T5146 T5145 nsubj we,observed
R3030 T5147 T5145 prep in,observed
R3031 T5148 T5149 poss our,mice
R3032 T5149 T5147 pobj mice,in
R3033 T5150 T5149 nmod Ptdsr,mice
R3034 T5151 T5150 punct -,Ptdsr
R3035 T5152 T5150 punct /,Ptdsr
R3036 T5153 T5150 punct -,Ptdsr
R3037 T5154 T5104 punct .,found
R3042 T5402 T5403 nsubjpass Loss,associated
R3043 T5404 T5402 prep of,Loss
R3044 T5405 T5406 compound Ptdsr,activity
R3045 T5406 T5404 pobj activity,of
R3046 T5407 T5403 auxpass is,associated
R3047 T5408 T5403 prep with,associated
R3048 T5409 T5408 pobj defects,with
R3049 T5410 T5409 prep in,defects
R3050 T5411 T5412 amod ocular,development
R3051 T5412 T5410 pobj development,in
R3052 T5413 T5403 cc and,associated
R3053 T5414 T5415 aux can,lead
R3054 T5415 T5403 conj lead,associated
R3055 T5416 T5415 prep to,lead
R3056 T5417 T5416 pobj formation,to
R3057 T5418 T5417 prep of,formation
R3058 T5419 T5420 amod ectopic,structures
R3059 T5420 T5418 pobj structures,of
R3060 T5421 T5420 compound eye,structures
R3061 T5423 T5424 prep By,differentiate
R3062 T5425 T5426 amod gross,morphology
R3063 T5426 T5423 pobj morphology,By
R3064 T5427 T5424 nsubj we,differentiate
R3065 T5428 T5424 aux could,differentiate
R3066 T5429 T5430 nummod two,classes
R3067 T5430 T5424 dobj classes,differentiate
R3068 T5431 T5430 prep of,classes
R3069 T5432 T5433 compound Ptdsr,mutants
R3070 T5433 T5431 pobj mutants,of
R3071 T5434 T5430 punct : ,classes
R3072 T5435 T5430 appos those,classes
R3073 T5436 T5437 dep that,appeared
R3074 T5437 T5435 relcl appeared,those
R3075 T5438 T5437 oprd normal,appeared
R3076 T5439 T5437 prep with,appeared
R3077 T5440 T5441 det both,eyes
R3078 T5441 T5439 pobj eyes,with
R3079 T5442 T5441 amod present,eyes
R3080 T5443 T5444 punct (,Figure
R3081 T5444 T5437 parataxis Figure,appeared
R3082 T5445 T5444 nummod 4,Figure
R3083 T5446 T5444 punct ),Figure
R3084 T5447 T5435 cc and,those
R3085 T5448 T5435 conj those,those
R3086 T5449 T5450 dep that,affected
R3087 T5450 T5448 relcl affected,those
R3088 T5451 T5450 auxpass were,affected
R3089 T5452 T5450 advmod severely,affected
R3090 T5453 T5450 cc and,affected
R3091 T5454 T5450 conj displayed,affected
R3092 T5455 T5456 amod uni,anophthalmia
R3093 T5456 T5454 dobj anophthalmia,displayed
R3094 T5457 T5455 punct -,uni
R3095 T5458 T5455 cc or,uni
R3096 T5459 T5455 conj bilateral,uni
R3097 T5460 T5461 punct (,Figure
R3098 T5461 T5454 parataxis Figure,displayed
R3099 T5462 T5461 nummod 5,Figure
R3100 T5463 T5461 punct ),Figure
R3101 T5464 T5424 punct .,differentiate
R3102 T5466 T5467 nsubj Analysis,revealed
R3103 T5468 T5466 prep of,Analysis
R3104 T5469 T5470 amod normal,embryos
R3105 T5470 T5468 pobj embryos,of
R3106 T5471 T5469 cc or,normal
R3107 T5472 T5473 advmod mildly,affected
R3108 T5473 T5469 conj affected,normal
R3109 T5474 T5475 det no,differences
R3110 T5475 T5467 dobj differences,revealed
R3111 T5476 T5475 prep between,differences
R3112 T5477 T5478 amod mutant,embryos
R3113 T5478 T5476 pobj embryos,between
R3114 T5479 T5477 cc and,mutant
R3115 T5480 T5481 amod wild,type
R3116 T5481 T5477 conj type,mutant
R3117 T5482 T5481 punct -,type
R3118 T5483 T5467 prep in,revealed
R3119 T5484 T5485 det the,differentiation
R3120 T5485 T5483 pobj differentiation,in
R3121 T5486 T5485 prep of,differentiation
R3122 T5487 T5488 det the,eye
R3123 T5488 T5486 pobj eye,of
R3124 T5489 T5488 amod developing,eye
R3125 T5490 T5467 prep until,revealed
R3126 T5491 T5490 pobj E16.5,until
R3127 T5492 T5467 punct .,revealed
R3128 T5494 T5495 prep In,displayed
R3129 T5496 T5497 det both,genotypes
R3130 T5497 T5494 pobj genotypes,In
R3131 T5498 T5495 punct ", ",displayed
R3132 T5499 T5500 amod inner,layers
R3133 T5500 T5495 nsubj layers,displayed
R3134 T5501 T5499 cc and,inner
R3135 T5502 T5499 conj outer,inner
R3136 T5503 T5500 prep of,layers
R3137 T5504 T5505 det the,retina
R3138 T5505 T5503 pobj retina,of
R3139 T5506 T5507 det a,status
R3140 T5507 T5495 dobj status,displayed
R3141 T5508 T5507 amod comparable,status
R3142 T5509 T5507 compound differentiation,status
R3143 T5510 T5495 punct ", ",displayed
R3144 T5511 T5512 mark as,shown
R3145 T5512 T5495 advcl shown,displayed
R3146 T5513 T5512 punct ", ",shown
R3147 T5514 T5512 prep for,shown
R3148 T5515 T5514 pobj example,for
R3149 T5516 T5512 punct ", ",shown
R3150 T5517 T5512 prep at,shown
R3151 T5518 T5517 pobj E12.5,at
R3152 T5519 T5520 punct (,e
R3153 T5520 T5495 parataxis e,displayed
R3154 T5521 T5520 nmod Figure,e
R3155 T5522 T5520 nmod 4a,e
R3156 T5523 T5520 punct ",",e
R3157 T5524 T5520 punct ),e
R3158 T5525 T5495 punct .,displayed
R3159 T5527 T5528 prep At,were
R3160 T5529 T5530 compound day,E16.5
R3161 T5530 T5527 pobj E16.5,At
R3162 T5531 T5528 punct ", ",were
R3163 T5532 T5528 advmod however,were
R3164 T5533 T5528 punct ", ",were
R3165 T5534 T5535 amod retinal,layers
R3166 T5535 T5528 nsubj layers,were
R3167 T5536 T5535 prep in,layers
R3168 T5537 T5538 nmod Ptdsr,embryos
R3169 T5538 T5536 pobj embryos,in
R3170 T5539 T5537 punct -,Ptdsr
R3171 T5540 T5537 punct /,Ptdsr
R3172 T5541 T5537 punct -,Ptdsr
R3173 T5542 T5543 advmod much,thinner
R3174 T5543 T5528 acomp thinner,were
R3175 T5544 T5543 prep than,thinner
R3176 T5545 T5544 prep in,than
R3177 T5546 T5547 amod wild,type
R3178 T5547 T5549 compound type,embryos
R3179 T5548 T5547 punct -,type
R3180 T5549 T5545 pobj embryos,in
R3181 T5550 T5528 punct ", ",were
R3182 T5551 T5528 conj contained,were
R3183 T5552 T5553 amod fewer,cells
R3184 T5553 T5551 dobj cells,contained
R3185 T5554 T5551 cc and,contained
R3186 T5555 T5556 auxpass were,reduced
R3187 T5556 T5551 conj reduced,contained
R3188 T5557 T5556 advmod greatly,reduced
R3189 T5558 T5556 prep in,reduced
R3190 T5559 T5558 pobj size,in
R3191 T5560 T5561 punct (,f
R3192 T5561 T5556 parataxis f,reduced
R3193 T5562 T5561 nmod Figure,f
R3194 T5563 T5561 nmod 4b,f
R3195 T5564 T5561 punct ",",f
R3196 T5565 T5561 punct ),f
R3197 T5566 T5528 punct .,were
R3198 T5568 T5569 nsubj Comparison,revealed
R3199 T5570 T5568 prep of,Comparison
R3200 T5571 T5572 det the,structures
R3201 T5572 T5570 pobj structures,of
R3202 T5573 T5572 amod retinal,structures
R3203 T5574 T5572 prep of,structures
R3204 T5575 T5576 nmod Ptdsr,embryos
R3205 T5576 T5574 pobj embryos,of
R3206 T5577 T5575 punct +,Ptdsr
R3207 T5578 T5575 punct /,Ptdsr
R3208 T5579 T5575 punct +,Ptdsr
R3209 T5580 T5575 cc and,Ptdsr
R3210 T5581 T5575 conj Ptdsr,Ptdsr
R3211 T5582 T5581 punct -,Ptdsr
R3212 T5583 T5581 punct /,Ptdsr
R3213 T5584 T5581 punct -,Ptdsr
R3214 T5585 T5586 mark that,were
R3215 T5586 T5569 ccomp were,revealed
R3216 T5587 T5588 det all,layers
R3217 T5588 T5586 nsubj layers,were
R3218 T5589 T5588 nummod four,layers
R3219 T5590 T5588 amod retinal,layers
R3220 T5591 T5586 acomp present,were
R3221 T5592 T5586 prep in,were
R3222 T5593 T5594 compound Ptdsr,knockout
R3223 T5594 T5596 compound knockout,mice
R3224 T5595 T5594 punct -,knockout
R3225 T5596 T5592 pobj mice,in
R3226 T5597 T5596 prep at,mice
R3227 T5598 T5597 pobj E16.5,at
R3228 T5599 T5600 punct (,f
R3229 T5600 T5569 parataxis f,revealed
R3230 T5601 T5600 nmod Figure,f
R3231 T5602 T5600 nmod 4b,f
R3232 T5603 T5600 punct ",",f
R3233 T5604 T5600 punct ),f
R3234 T5605 T5569 punct .,revealed
R3235 T5607 T5608 prep At,were
R3236 T5609 T5607 pobj E18.5,At
R3237 T5610 T5611 punct (,g
R3238 T5611 T5609 parataxis g,E18.5
R3239 T5612 T5611 nmod Figure,g
R3240 T5613 T5611 nmod 4c,g
R3241 T5614 T5611 punct ",",g
R3242 T5615 T5611 punct ),g
R3243 T5616 T5607 cc and,At
R3244 T5617 T5607 conj in,At
R3245 T5618 T5619 amod neonatal,animals
R3246 T5619 T5617 pobj animals,in
R3247 T5620 T5621 punct (,h
R3248 T5621 T5608 parataxis h,were
R3249 T5622 T5623 amod postnatal,P0
R3250 T5623 T5621 dep P0,h
R3251 T5624 T5623 compound day,P0
R3252 T5625 T5621 punct ;,h
R3253 T5626 T5621 nmod Figure,h
R3254 T5627 T5621 nmod 4d,h
R3255 T5628 T5621 punct ",",h
R3256 T5629 T5621 punct ),h
R3257 T5630 T5608 punct ", ",were
R3258 T5631 T5632 det the,differences
R3259 T5632 T5608 nsubj differences,were
R3260 T5633 T5632 prep in,differences
R3261 T5634 T5635 amod retinal,differentiation
R3262 T5635 T5633 pobj differentiation,in
R3263 T5636 T5632 prep between,differences
R3264 T5637 T5638 nmod Ptdsr,mice
R3265 T5638 T5636 pobj mice,between
R3266 T5639 T5637 punct +,Ptdsr
R3267 T5640 T5637 punct /,Ptdsr
R3268 T5641 T5637 punct +,Ptdsr
R3269 T5642 T5637 cc and,Ptdsr
R3270 T5643 T5637 conj Ptdsr,Ptdsr
R3271 T5644 T5643 punct -,Ptdsr
R3272 T5645 T5643 punct /,Ptdsr
R3273 T5646 T5643 punct -,Ptdsr
R3274 T5647 T5608 advmod still,were
R3275 T5648 T5608 acomp evident,were
R3276 T5649 T5608 punct ", ",were
R3277 T5650 T5608 cc but,were
R3278 T5651 T5652 det the,reduction
R3279 T5652 T5654 nsubj reduction,was
R3280 T5653 T5652 compound size,reduction
R3281 T5654 T5608 conj was,were
R3282 T5655 T5652 prep of,reduction
R3283 T5656 T5657 det the,layers
R3284 T5657 T5655 pobj layers,of
R3285 T5658 T5657 amod retinal,layers
R3286 T5659 T5660 advmod less,pronounced
R3287 T5660 T5654 acomp pronounced,was
R3288 T5661 T5654 prep in,was
R3289 T5662 T5663 det the,mice
R3290 T5663 T5661 pobj mice,in
R3291 T5664 T5663 compound knockout,mice
R3292 T5665 T5608 punct .,were
R3293 T5667 T5668 npadvmod Ptdsr,deficient
R3294 T5668 T5670 amod deficient,animals
R3295 T5669 T5668 punct -,deficient
R3296 T5670 T5671 nsubj animals,seem
R3297 T5672 T5673 aux to,compensated
R3298 T5673 T5671 xcomp compensated,seem
R3299 T5674 T5673 aux have,compensated
R3300 T5675 T5673 prep for,compensated
R3301 T5676 T5677 det the,delay
R3302 T5677 T5675 pobj delay,for
R3303 T5678 T5677 amod marked,delay
R3304 T5679 T5677 prep in,delay
R3305 T5680 T5681 amod cellular,differentiation
R3306 T5681 T5679 pobj differentiation,in
R3307 T5682 T5681 cc and,differentiation
R3308 T5683 T5681 conj expansion,differentiation
R3309 T5684 T5683 prep of,expansion
R3310 T5685 T5686 amod retinal,layers
R3311 T5686 T5684 pobj layers,of
R3312 T5687 T5671 punct .,seem
R3313 T5689 T5690 compound Close,examination
R3314 T5690 T5691 nsubj examination,revealed
R3315 T5692 T5690 prep of,examination
R3316 T5693 T5694 amod retinal,structures
R3317 T5694 T5692 pobj structures,of
R3318 T5695 T5696 mark that,expanded
R3319 T5696 T5691 advcl expanded,revealed
R3320 T5697 T5698 det the,layer
R3321 T5698 T5696 nsubjpass layer,expanded
R3322 T5699 T5698 amod inner,layer
R3323 T5700 T5698 amod granular,layer
R3324 T5701 T5696 auxpass was,expanded
R3325 T5702 T5696 advmod still,expanded
R3326 T5703 T5696 advmod less,expanded
R3327 T5704 T5696 prep in,expanded
R3328 T5705 T5706 npadvmod Ptdsr,deficient
R3329 T5706 T5708 amod deficient,animals
R3330 T5707 T5706 punct -,deficient
R3331 T5708 T5704 pobj animals,in
R3332 T5709 T5696 punct ", ",expanded
R3333 T5710 T5696 advmod however,expanded
R3334 T5711 T5696 punct ", ",expanded
R3335 T5712 T5696 cc and,expanded
R3336 T5713 T5714 mark that,contained
R3337 T5714 T5696 conj contained,expanded
R3338 T5715 T5714 nsubj it,contained
R3339 T5716 T5717 amod fewer,cells
R3340 T5717 T5714 dobj cells,contained
R3341 T5718 T5714 cc and,contained
R3342 T5719 T5714 conj was,contained
R3343 T5720 T5719 advmod still,was
R3344 T5721 T5722 advmod severely,underdeveloped
R3345 T5722 T5719 acomp underdeveloped,was
R3346 T5723 T5719 prep in,was
R3347 T5724 T5723 pobj comparison,in
R3348 T5725 T5724 prep with,comparison
R3349 T5726 T5727 det the,layer
R3350 T5727 T5725 pobj layer,with
R3351 T5728 T5727 amod corresponding,layer
R3352 T5729 T5727 amod retinal,layer
R3353 T5730 T5727 prep in,layer
R3354 T5731 T5732 compound control,animals
R3355 T5732 T5730 pobj animals,in
R3356 T5733 T5734 punct (,4c
R3357 T5734 T5691 parataxis 4c,revealed
R3358 T5735 T5734 compound Figure,4c
R3359 T5736 T5734 punct ",",4c
R3360 T5737 T5734 conj 4g,4c
R3361 T5738 T5737 cc and,4g
R3362 T5739 T5737 conj 4d,4g
R3363 T5740 T5739 punct ",",4d
R3364 T5741 T5739 conj 4h,4d
R3365 T5742 T5734 punct ),4c
R3366 T5743 T5691 punct .,revealed
R3367 T5745 T5746 advmod Thus,had
R3368 T5747 T5746 punct ", ",had
R3369 T5748 T5749 advmod even,mildly
R3370 T5749 T5750 advmod mildly,affected
R3371 T5750 T5751 amod affected,mutants
R3372 T5751 T5746 nsubj mutants,had
R3373 T5752 T5751 nmod Ptdsr,mutants
R3374 T5753 T5752 punct -,Ptdsr
R3375 T5754 T5752 punct /,Ptdsr
R3376 T5755 T5752 punct -,Ptdsr
R3377 T5756 T5757 amod ocular,malformations
R3378 T5757 T5746 dobj malformations,had
R3379 T5758 T5757 prep with,malformations
R3380 T5759 T5758 pobj defects,with
R3381 T5760 T5759 prep in,defects
R3382 T5761 T5760 pobj differentiation,in
R3383 T5762 T5761 prep of,differentiation
R3384 T5763 T5764 amod retinal,structures
R3385 T5764 T5762 pobj structures,of
R3386 T5765 T5746 punct .,had
R3387 T5767 T5768 nsubj We,examined
R3388 T5769 T5768 advmod next,examined
R3389 T5770 T5771 nmod Ptdsr,embryos
R3390 T5771 T5768 dobj embryos,examined
R3391 T5772 T5770 punct -,Ptdsr
R3392 T5773 T5770 punct /,Ptdsr
R3393 T5774 T5770 punct -,Ptdsr
R3394 T5775 T5776 dep that,displayed
R3395 T5776 T5771 relcl displayed,embryos
R3396 T5777 T5778 amod unilateral,absence
R3397 T5778 T5776 dobj absence,displayed
R3398 T5779 T5777 cc or,unilateral
R3399 T5780 T5777 conj bilateral,unilateral
R3400 T5781 T5778 prep of,absence
R3401 T5782 T5781 pobj eyes,of
R3402 T5783 T5784 punct (,5a
R3403 T5784 T5776 parataxis 5a,displayed
R3404 T5785 T5784 compound Figure,5a
R3405 T5786 T5784 punct ),5a
R3406 T5787 T5768 prep by,examined
R3407 T5788 T5789 amod serial,sectioning
R3408 T5789 T5787 pobj sectioning,by
R3409 T5790 T5789 prep of,sectioning
R3410 T5791 T5792 amod whole,embryos
R3411 T5792 T5790 pobj embryos,of
R3412 T5793 T5768 punct .,examined
R3413 T5795 T5796 det These,embryos
R3414 T5796 T5797 nsubj embryos,showed
R3415 T5798 T5799 amod complex,malformations
R3416 T5799 T5797 dobj malformations,showed
R3417 T5800 T5799 prep of,malformations
R3418 T5801 T5802 det the,cup
R3419 T5802 T5800 pobj cup,of
R3420 T5803 T5802 amod optical,cup
R3421 T5804 T5799 punct ", ",malformations
R3422 T5805 T5799 prep including,malformations
R3423 T5806 T5805 pobj absence,including
R3424 T5807 T5806 prep of,absence
R3425 T5808 T5809 det the,lens
R3426 T5809 T5807 pobj lens,of
R3427 T5810 T5811 punct (,5b
R3428 T5811 T5797 parataxis 5b,showed
R3429 T5812 T5811 compound Figure,5b
R3430 T5813 T5811 punct ),5b
R3431 T5814 T5797 punct .,showed
R3432 T5816 T5817 advmod Most,surprisingly
R3433 T5817 T5818 advmod surprisingly,found
R3434 T5819 T5818 punct ", ",found
R3435 T5820 T5818 nsubj we,found
R3436 T5821 T5822 amod pigmented,cells
R3437 T5822 T5818 dobj cells,found
R3438 T5823 T5822 amod epithelial,cells
R3439 T5824 T5822 prep in,cells
R3440 T5825 T5826 det the,cavity
R3441 T5826 T5824 pobj cavity,in
R3442 T5827 T5826 amod nasal,cavity
R3443 T5828 T5826 prep of,cavity
R3444 T5829 T5830 det all,mice
R3445 T5830 T5828 pobj mice,of
R3446 T5831 T5832 compound Ptdsr,knockout
R3447 T5832 T5830 compound knockout,mice
R3448 T5833 T5832 punct -,knockout
R3449 T5834 T5830 prep with,mice
R3450 T5835 T5834 pobj anophthalmia,with
R3451 T5836 T5837 dep that,analyzed
R3452 T5837 T5822 relcl analyzed,cells
R3453 T5838 T5837 auxpass were,analyzed
R3454 T5839 T5837 advmod histopathologically,analyzed
R3455 T5840 T5818 punct .,found
R3456 T5842 T5843 nsubj We,identify
R3457 T5844 T5843 aux could,identify
R3458 T5845 T5846 amod black,colored
R3459 T5846 T5848 amod colored,cells
R3460 T5847 T5846 punct -,colored
R3461 T5848 T5843 dobj cells,identify
R3462 T5849 T5848 amod pigmented,cells
R3463 T5850 T5848 acl embedded,cells
R3464 T5851 T5850 prep in,embedded
R3465 T5852 T5853 det the,epithelium
R3466 T5853 T5851 pobj epithelium,in
R3467 T5854 T5853 prep of,epithelium
R3468 T5855 T5856 det the,sinus
R3469 T5856 T5854 pobj sinus,of
R3470 T5857 T5856 amod maxillary,sinus
R3471 T5858 T5859 dep that,resembled
R3472 T5859 T5848 relcl resembled,cells
R3473 T5860 T5861 amod presumptive,epithelium
R3474 T5861 T5859 dobj epithelium,resembled
R3475 T5862 T5863 amod retinal,pigmented
R3476 T5863 T5861 amod pigmented,epithelium
R3477 T5864 T5863 punct -,pigmented
R3478 T5865 T5866 punct (,c
R3479 T5866 T5843 parataxis c,identify
R3480 T5867 T5866 nmod Figure,c
R3481 T5868 T5866 nmod 5b,c
R3482 T5869 T5866 punct ",",c
R3483 T5870 T5866 punct ),c
R3484 T5871 T5843 punct .,identify
R3485 T5873 T5874 nsubj Examination,revealed
R3486 T5875 T5873 prep of,Examination
R3487 T5876 T5877 amod consecutive,sections
R3488 T5877 T5875 pobj sections,of
R3489 T5878 T5877 amod serial,sections
R3490 T5879 T5880 det the,formation
R3491 T5880 T5874 dobj formation,revealed
R3492 T5881 T5880 prep of,formation
R3493 T5882 T5883 det a,structure
R3494 T5883 T5881 pobj structure,of
R3495 T5884 T5883 amod primitive,structure
R3496 T5885 T5883 compound eye,structure
R3497 T5886 T5880 punct ", ",formation
R3498 T5887 T5880 prep with,formation
R3499 T5888 T5887 pobj induction,with
R3500 T5889 T5888 cc and,induction
R3501 T5890 T5891 amod subsequent,proliferation
R3502 T5891 T5888 conj proliferation,induction
R3503 T5892 T5891 prep of,proliferation
R3504 T5893 T5894 amod ectopic,tissue
R3505 T5894 T5892 pobj tissue,of
R3506 T5895 T5894 amod mesenchymal,tissue
R3507 T5896 T5897 advmod immediately,adjacent
R3508 T5897 T5894 amod adjacent,tissue
R3509 T5898 T5897 prep to,adjacent
R3510 T5899 T5900 det the,epithelium
R3511 T5900 T5898 pobj epithelium,to
R3512 T5901 T5900 amod displaced,epithelium
R3513 T5902 T5900 amod pigmented,epithelium
R3514 T5903 T5904 punct (,5d
R3515 T5904 T5874 parataxis 5d,revealed
R3516 T5905 T5904 compound Figure,5d
R3517 T5906 T5904 punct ),5d
R3518 T5907 T5874 punct .,revealed
R3519 T5909 T5910 det This,structure
R3520 T5910 T5911 nsubjpass structure,induced
R3521 T5912 T5911 auxpass was,induced
R3522 T5913 T5911 advmod clearly,induced
R3523 T5914 T5911 advmod ectopically,induced
R3524 T5915 T5911 punct ", ",induced
R3525 T5916 T5911 cc and,induced
R3526 T5917 T5918 nsubj we,failed
R3527 T5918 T5911 conj failed,induced
R3528 T5919 T5920 aux to,identify
R3529 T5920 T5918 xcomp identify,failed
R3530 T5921 T5922 amod similar,changes
R3531 T5922 T5920 dobj changes,identify
R3532 T5923 T5920 prep in,identify
R3533 T5924 T5923 pobj any,in
R3534 T5925 T5924 prep of,any
R3535 T5926 T5927 det the,embryos
R3536 T5927 T5925 pobj embryos,of
R3537 T5928 T5929 amod wild,type
R3538 T5929 T5927 compound type,embryos
R3539 T5930 T5929 punct -,type
R3540 T5931 T5918 punct .,failed
R3541 T5933 T5934 prep In,observed
R3542 T5935 T5933 pobj summary,In
R3543 T5936 T5934 punct ", ",observed
R3544 T5937 T5934 nsubj we,observed
R3545 T5938 T5939 det a,range
R3546 T5939 T5934 dobj range,observed
R3547 T5940 T5939 amod wide,range
R3548 T5941 T5939 prep of,range
R3549 T5942 T5943 amod ocular,malformations
R3550 T5943 T5941 pobj malformations,of
R3551 T5944 T5939 prep in,range
R3552 T5945 T5946 npadvmod Ptdsr,deficient
R3553 T5946 T5948 amod deficient,mice
R3554 T5947 T5946 punct -,deficient
R3555 T5948 T5944 pobj mice,in
R3556 T5949 T5950 dep that,ranged
R3557 T5950 T5939 relcl ranged,range
R3558 T5951 T5950 prep from,ranged
R3559 T5952 T5953 compound differentiation,defects
R3560 T5953 T5951 pobj defects,from
R3561 T5954 T5953 prep in,defects
R3562 T5955 T5956 amod retinal,layers
R3563 T5956 T5954 pobj layers,in
R3564 T5957 T5956 compound cell,layers
R3565 T5958 T5959 punct (,layer
R3566 T5959 T5953 parataxis layer,defects
R3567 T5960 T5959 prep for,layer
R3568 T5961 T5960 pobj example,for
R3569 T5962 T5959 punct ", ",layer
R3570 T5963 T5959 det the,layer
R3571 T5964 T5959 amod inner,layer
R3572 T5965 T5959 amod granular,layer
R3573 T5966 T5959 punct ),layer
R3574 T5967 T5953 prep in,defects
R3575 T5968 T5969 advmod mildly,affected
R3576 T5969 T5970 amod affected,homozygotes
R3577 T5970 T5967 pobj homozygotes,in
R3578 T5971 T5951 prep to,from
R3579 T5972 T5971 pobj anophthalmia,to
R3580 T5973 T5972 prep in,anophthalmia
R3581 T5974 T5975 advmod severely,affected
R3582 T5975 T5976 amod affected,mice
R3583 T5976 T5973 pobj mice,in
R3584 T5977 T5976 nmod Ptdsr,mice
R3585 T5978 T5977 punct -,Ptdsr
R3586 T5979 T5977 punct /,Ptdsr
R3587 T5980 T5977 punct -,Ptdsr
R3588 T5981 T5982 dep that,associated
R3589 T5982 T5972 relcl associated,anophthalmia
R3590 T5983 T5982 auxpass was,associated
R3591 T5984 T5982 prep with,associated
R3592 T5985 T5984 pobj induction,with
R3593 T5986 T5985 prep of,induction
R3594 T5987 T5988 amod ectopic,structures
R3595 T5988 T5986 pobj structures,of
R3596 T5989 T5988 compound eye,structures
R3597 T5990 T5985 prep in,induction
R3598 T5991 T5992 amod nasal,cavities
R3599 T5992 T5990 pobj cavities,in
R3600 T5993 T5934 punct .,observed
R3613 T6746 T6747 nsubj Phagocytosis,is
R3614 T6748 T6746 cc and,Phagocytosis
R3615 T6749 T6746 conj clearance,Phagocytosis
R3616 T6750 T6746 prep of,Phagocytosis
R3617 T6751 T6752 amod apoptotic,cells
R3618 T6752 T6750 pobj cells,of
R3619 T6753 T6747 acomp normal,is
R3620 T6754 T6747 prep in,is
R3621 T6755 T6756 npadvmod Ptdsr,deficient
R3622 T6756 T6758 amod deficient,mice
R3623 T6757 T6756 punct -,deficient
R3624 T6758 T6754 pobj mice,in
R3625 T6760 T6761 nsubj We,tested
R3626 T6762 T6761 advmod next,tested
R3627 T6763 T6764 mark whether,required
R3628 T6764 T6761 ccomp required,tested
R3629 T6765 T6764 nsubjpass Ptdsr,required
R3630 T6766 T6764 auxpass is,required
R3631 T6767 T6764 advmod functionally,required
R3632 T6768 T6764 prep for,required
R3633 T6769 T6770 det the,clearance
R3634 T6770 T6768 pobj clearance,for
R3635 T6771 T6770 prep of,clearance
R3636 T6772 T6773 amod apoptotic,cells
R3637 T6773 T6771 pobj cells,of
R3638 T6774 T6761 punct .,tested
R3639 T6776 T6777 nsubj We,started
R3640 T6778 T6777 prep with,started
R3641 T6779 T6780 det an,investigation
R3642 T6780 T6778 pobj investigation,with
R3643 T6781 T6780 prep of,investigation
R3644 T6782 T6783 compound cell,death
R3645 T6783 T6781 pobj death,of
R3646 T6784 T6785 advmod in,vivo
R3647 T6785 T6783 advmod vivo,death
R3648 T6786 T6783 prep in,death
R3649 T6787 T6788 det the,areas
R3650 T6788 T6786 pobj areas,in
R3651 T6789 T6788 amod interdigital,areas
R3652 T6790 T6788 prep of,areas
R3653 T6791 T6792 det the,limbs
R3654 T6792 T6790 pobj limbs,of
R3655 T6793 T6792 amod developing,limbs
R3656 T6794 T6777 punct .,started
R3657 T6796 T6797 nsubj Apoptosis,occurs
R3658 T6798 T6796 prep of,Apoptosis
R3659 T6799 T6800 amod interdigital,cells
R3660 T6800 T6798 pobj cells,of
R3661 T6801 T6796 prep in,Apoptosis
R3662 T6802 T6803 det the,mesenchyme
R3663 T6803 T6801 pobj mesenchyme,in
R3664 T6804 T6803 amod distal,mesenchyme
R3665 T6805 T6803 prep of,mesenchyme
R3666 T6806 T6807 compound limb,buds
R3667 T6807 T6805 pobj buds,of
R3668 T6808 T6809 advmod most,prominently
R3669 T6809 T6797 advmod prominently,occurs
R3670 T6810 T6797 prep from,occurs
R3671 T6811 T6812 amod developmental,E12.0
R3672 T6812 T6810 pobj E12.0,from
R3673 T6813 T6812 compound stages,E12.0
R3674 T6814 T6812 prep to,E12.0
R3675 T6815 T6814 pobj E13.5,to
R3676 T6816 T6797 cc and,occurs
R3677 T6817 T6818 aux can,examined
R3678 T6818 T6797 conj examined,occurs
R3679 T6819 T6818 auxpass be,examined
R3680 T6820 T6818 advmod easily,examined
R3681 T6821 T6822 advmod in,situ
R3682 T6822 T6818 advmod situ,examined
R3683 T6823 T6818 prep by,examined
R3684 T6824 T6825 amod whole,mount
R3685 T6825 T6827 nmod mount,end
R3686 T6826 T6825 punct -,mount
R3687 T6827 T6823 pobj end,by
R3688 T6828 T6827 amod terminal,end
R3689 T6829 T6827 nmod deoxynucleotide,end
R3690 T6830 T6831 npadvmod transferase,mediated
R3691 T6831 T6827 amod mediated,end
R3692 T6832 T6831 punct -,mediated
R3693 T6833 T6827 compound UTP,end
R3694 T6834 T6827 punct -,end
R3695 T6835 T6827 amod labeling,end
R3696 T6836 T6827 punct (,end
R3697 T6837 T6827 appos TUNEL,end
R3698 T6838 T6797 punct ),occurs
R3699 T6839 T6797 punct .,occurs
R3700 T6841 T6842 nsubj We,compared
R3701 T6843 T6844 det the,pattern
R3702 T6844 T6842 dobj pattern,compared
R3703 T6845 T6844 prep of,pattern
R3704 T6846 T6847 amod interdigital,death
R3705 T6847 T6845 pobj death,of
R3706 T6848 T6847 compound cell,death
R3707 T6849 T6842 prep in,compared
R3708 T6850 T6851 amod fore,buds
R3709 T6851 T6849 pobj buds,in
R3710 T6852 T6850 cc and,fore
R3711 T6853 T6850 conj hind,fore
R3712 T6854 T6851 compound limb,buds
R3713 T6855 T6851 prep from,buds
R3714 T6856 T6857 nmod Ptdsr,mice
R3715 T6857 T6855 pobj mice,from
R3716 T6858 T6856 punct -,Ptdsr
R3717 T6859 T6856 punct /,Ptdsr
R3718 T6860 T6856 punct -,Ptdsr
R3719 T6861 T6862 punct (,3
R3720 T6862 T6856 parataxis 3,Ptdsr
R3721 T6863 T6862 nsubj n,3
R3722 T6864 T6862 punct =,3
R3723 T6865 T6862 punct ),3
R3724 T6866 T6856 cc and,Ptdsr
R3725 T6867 T6856 conj Ptdsr,Ptdsr
R3726 T6868 T6867 punct +,Ptdsr
R3727 T6869 T6867 punct /,Ptdsr
R3728 T6870 T6867 punct +,Ptdsr
R3729 T6871 T6872 punct (,3
R3730 T6872 T6867 parataxis 3,Ptdsr
R3731 T6873 T6872 nsubj n,3
R3732 T6874 T6872 punct =,3
R3733 T6875 T6872 punct ),3
R3734 T6876 T6857 prep at,mice
R3735 T6877 T6876 pobj E12.5,at
R3736 T6878 T6877 cc and,E12.5
R3737 T6879 T6877 conj E13.5,E12.5
R3738 T6880 T6842 punct .,compared
R3739 T6882 T6883 det No,differences
R3740 T6883 T6884 nsubjpass differences,observed
R3741 T6885 T6883 prep in,differences
R3742 T6886 T6885 pobj accumulation,in
R3743 T6887 T6886 prep of,accumulation
R3744 T6888 T6889 npadvmod TUNEL,positive
R3745 T6889 T6891 amod positive,corpses
R3746 T6890 T6889 punct -,positive
R3747 T6891 T6887 pobj corpses,of
R3748 T6892 T6891 compound cell,corpses
R3749 T6893 T6884 auxpass were,observed
R3750 T6894 T6884 prep between,observed
R3751 T6895 T6896 det the,genotypes
R3752 T6896 T6894 pobj genotypes,between
R3753 T6897 T6896 nummod two,genotypes
R3754 T6898 T6899 punct (,6a
R3755 T6899 T6884 parataxis 6a,observed
R3756 T6900 T6899 compound Figure,6a
R3757 T6901 T6899 punct ),6a
R3758 T6902 T6884 punct .,observed
R3759 T6904 T6905 det The,kinetics
R3760 T6905 T6906 nsubj kinetics,was
R3761 T6907 T6905 prep of,kinetics
R3762 T6908 T6909 compound cell,death
R3763 T6909 T6910 compound death,occurrence
R3764 T6910 T6907 pobj occurrence,of
R3765 T6911 T6910 cc and,occurrence
R3766 T6912 T6910 conj regression,occurrence
R3767 T6913 T6912 prep of,regression
R3768 T6914 T6915 det the,web
R3769 T6915 T6913 pobj web,of
R3770 T6916 T6915 amod interdigital,web
R3771 T6917 T6906 acomp similar,was
R3772 T6918 T6906 prep in,was
R3773 T6919 T6920 amod wild,type
R3774 T6920 T6922 nmod type,littermates
R3775 T6921 T6920 punct -,type
R3776 T6922 T6918 pobj littermates,in
R3777 T6923 T6920 cc and,type
R3778 T6924 T6920 conj mutant,type
R3779 T6925 T6906 punct ", ",was
R3780 T6926 T6906 advcl providing,was
R3781 T6927 T6928 det no,evidence
R3782 T6928 T6926 dobj evidence,providing
R3783 T6929 T6930 mark that,associated
R3784 T6930 T6928 acl associated,evidence
R3785 T6931 T6932 compound Ptdsr,deficiency
R3786 T6932 T6930 nsubjpass deficiency,associated
R3787 T6933 T6932 punct -,deficiency
R3788 T6934 T6930 auxpass is,associated
R3789 T6935 T6930 prep with,associated
R3790 T6936 T6937 amod impaired,clearance
R3791 T6937 T6935 pobj clearance,with
R3792 T6938 T6937 prep of,clearance
R3793 T6939 T6940 amod apoptotic,cells
R3794 T6940 T6938 pobj cells,of
R3795 T6941 T6940 amod interdigital,cells
R3796 T6942 T6937 prep during,clearance
R3797 T6943 T6944 compound limb,development
R3798 T6944 T6942 pobj development,during
R3799 T6945 T6906 punct .,was
R3800 T6947 T6948 aux To,investigate
R3801 T6948 T6949 advcl investigate,stained
R3802 T6950 T6948 advmod further,investigate
R3803 T6951 T6952 mark whether,impaired
R3804 T6952 T6948 ccomp impaired,investigate
R3805 T6953 T6952 nsubjpass removal,impaired
R3806 T6954 T6953 prep of,removal
R3807 T6955 T6956 amod apoptotic,cells
R3808 T6956 T6954 pobj cells,of
R3809 T6957 T6952 auxpass is,impaired
R3810 T6958 T6952 prep in,impaired
R3811 T6959 T6960 nmod Ptdsr,mice
R3812 T6960 T6958 pobj mice,in
R3813 T6961 T6959 punct -,Ptdsr
R3814 T6962 T6959 punct /,Ptdsr
R3815 T6963 T6959 punct -,Ptdsr
R3816 T6964 T6949 punct ", ",stained
R3817 T6965 T6949 nsubj we,stained
R3818 T6966 T6949 advmod immunohistochemically,stained
R3819 T6967 T6949 prep for,stained
R3820 T6968 T6969 amod activated,caspase
R3821 T6969 T6967 pobj caspase,for
R3822 T6970 T6969 nummod 3,caspase
R3823 T6971 T6969 punct (,caspase
R3824 T6972 T6969 appos aCasp3,caspase
R3825 T6973 T6949 punct ),stained
R3826 T6974 T6949 cc and,stained
R3827 T6975 T6949 conj analyzed,stained
R3828 T6976 T6977 amod additional,organs
R3829 T6977 T6975 dobj organs,analyzed
R3830 T6978 T6977 cc and,organs
R3831 T6979 T6977 conj tissues,organs
R3832 T6980 T6981 advmod where,plays
R3833 T6981 T6977 relcl plays,organs
R3834 T6982 T6981 nsubj apoptosis,plays
R3835 T6983 T6984 det a,role
R3836 T6984 T6981 dobj role,plays
R3837 T6985 T6984 amod crucial,role
R3838 T6986 T6981 prep in,plays
R3839 T6987 T6988 compound tissue,remodeling
R3840 T6988 T6986 pobj remodeling,in
R3841 T6989 T6981 prep during,plays
R3842 T6990 T6989 pobj development,during
R3843 T6991 T6949 punct .,stained
R3844 T6993 T6994 advcl Starting,analyzed
R3845 T6995 T6993 prep at,Starting
R3846 T6996 T6995 pobj E12.5,at
R3847 T6997 T6994 punct ", ",analyzed
R3848 T6998 T6994 nsubj we,analyzed
R3849 T6999 T6994 cc and,analyzed
R3850 T7000 T6994 conj compared,analyzed
R3851 T7001 T7002 det the,number
R3852 T7002 T7000 dobj number,compared
R3853 T7003 T7002 cc and,number
R3854 T7004 T7002 conj distribution,number
R3855 T7005 T7002 prep of,number
R3856 T7006 T7007 npadvmod aCasp3,positive
R3857 T7007 T7009 amod positive,cells
R3858 T7008 T7007 punct -,positive
R3859 T7009 T7005 pobj cells,of
R3860 T7010 T7002 prep in,number
R3861 T7011 T7012 quantmod over,140
R3862 T7012 T7013 nummod 140,sections
R3863 T7013 T7010 pobj sections,in
R3864 T7014 T7013 amod serial,sections
R3865 T7015 T7013 prep of,sections
R3866 T7016 T7017 nummod three,type
R3867 T7017 T7015 pobj type,of
R3868 T7018 T7017 amod wild,type
R3869 T7019 T7017 punct -,type
R3870 T7020 T7017 cc and,type
R3871 T7021 T7022 nummod six,Ptdsr
R3872 T7022 T7023 nmod Ptdsr,embryos
R3873 T7023 T7017 conj embryos,type
R3874 T7024 T7022 punct -,Ptdsr
R3875 T7025 T7022 punct /,Ptdsr
R3876 T7026 T7022 punct -,Ptdsr
R3877 T7027 T7013 prep in,sections
R3878 T7028 T7029 amod consecutive,sections
R3879 T7029 T7027 pobj sections,in
R3880 T7030 T7028 cc and,consecutive
R3881 T7031 T7028 conj corresponding,consecutive
R3882 T7032 T6994 punct .,analyzed
R3883 T7034 T7035 det The,sections
R3884 T7035 T7037 nsubjpass sections,separated
R3885 T7036 T7035 amod sagittal,sections
R3886 T7038 T7037 auxpass were,separated
R3887 T7039 T7037 agent by,separated
R3888 T7040 T7041 nummod 5,μm
R3889 T7041 T7039 pobj μm,by
R3890 T7042 T7037 punct ", ",separated
R3891 T7043 T7037 advcl allowing,separated
R3892 T7044 T7045 det a,analysis
R3893 T7045 T7043 dobj analysis,allowing
R3894 T7046 T7045 amod detailed,analysis
R3895 T7047 T7045 prep of,analysis
R3896 T7048 T7047 pobj apoptosis,of
R3897 T7049 T7045 prep in,analysis
R3898 T7050 T7051 amod several,organs
R3899 T7051 T7049 pobj organs,in
R3900 T7052 T7051 cc and,organs
R3901 T7053 T7051 conj tissues,organs
R3902 T7054 T7037 punct .,separated
R3903 T7056 T7057 compound Tissue,restructuring
R3904 T7057 T7058 nsubj restructuring,occurred
R3905 T7059 T7057 prep by,restructuring
R3906 T7060 T7061 amod programmed,death
R3907 T7061 T7059 pobj death,by
R3908 T7062 T7061 compound cell,death
R3909 T7063 T7064 advmod most,notably
R3910 T7064 T7065 advmod notably,within
R3911 T7065 T7058 prep within,occurred
R3912 T7066 T7067 det the,part
R3913 T7067 T7065 pobj part,within
R3914 T7068 T7067 amod ventral,part
R3915 T7069 T7067 prep of,part
R3916 T7070 T7071 det the,tube
R3917 T7071 T7069 pobj tube,of
R3918 T7072 T7071 amod neural,tube
R3919 T7073 T7074 punct (,f
R3920 T7074 T7071 parataxis f,tube
R3921 T7075 T7074 nmod Figure,f
R3922 T7076 T7074 nmod 6b,f
R3923 T7077 T7074 punct ",",f
R3924 T7078 T7074 punct ),f
R3925 T7079 T7065 cc and,within
R3926 T7080 T7065 conj in,within
R3927 T7081 T7082 det the,ganglia
R3928 T7082 T7080 pobj ganglia,in
R3929 T7083 T7082 amod developing,ganglia
R3930 T7084 T7082 amod paravertebral,ganglia
R3931 T7085 T7086 punct (,h
R3932 T7086 T7082 parataxis h,ganglia
R3933 T7087 T7086 nmod Figure,h
R3934 T7088 T7086 nmod 6d,h
R3935 T7089 T7086 punct ",",h
R3936 T7090 T7086 punct ),h
R3937 T7091 T7058 prep with,occurred
R3938 T7092 T7093 amod many,cells
R3939 T7093 T7095 nsubj cells,being
R3940 T7094 T7093 amod apoptotic,cells
R3941 T7095 T7091 pcomp being,with
R3942 T7096 T7095 acomp present,being
R3943 T7097 T7058 punct .,occurred
R3944 T7099 T7100 prep In,is
R3945 T7101 T7102 det these,tissues
R3946 T7102 T7099 pobj tissues,In
R3947 T7103 T7100 nsubj Ptdsr,is
R3948 T7104 T7105 advmod highly,expressed
R3949 T7105 T7100 acomp expressed,is
R3950 T7106 T7100 prep at,is
R3951 T7107 T7106 pobj E12.5,at
R3952 T7108 T7109 punct (,2c
R3953 T7109 T7107 parataxis 2c,E12.5
R3954 T7110 T7109 compound Figure,2c
R3955 T7111 T7109 punct ),2c
R3956 T7112 T7100 cc but,is
R3957 T7113 T7114 nsubj we,observed
R3958 T7114 T7100 conj observed,is
R3959 T7115 T7116 det no,difference
R3960 T7116 T7114 dobj difference,observed
R3961 T7117 T7116 prep in,difference
R3962 T7118 T7119 det the,number
R3963 T7119 T7117 pobj number,in
R3964 T7120 T7119 cc or,number
R3965 T7121 T7119 conj distribution,number
R3966 T7122 T7119 prep of,number
R3967 T7123 T7124 amod apoptotic,cells
R3968 T7124 T7122 pobj cells,of
R3969 T7125 T7116 prep in,difference
R3970 T7126 T7127 nmod Ptdsr,embryos
R3971 T7127 T7125 pobj embryos,in
R3972 T7128 T7126 punct +,Ptdsr
R3973 T7129 T7126 punct /,Ptdsr
R3974 T7130 T7126 punct +,Ptdsr
R3975 T7131 T7126 cc and,Ptdsr
R3976 T7132 T7126 conj Ptdsr,Ptdsr
R3977 T7133 T7132 punct -,Ptdsr
R3978 T7134 T7132 punct /,Ptdsr
R3979 T7135 T7132 punct -,Ptdsr
R3980 T7136 T7114 punct .,observed
R3981 T7138 T7139 det The,same
R3982 T7139 T7140 nsubj same,was
R3983 T7140 T7141 ccomp was,were
R3984 T7142 T7140 acomp true,was
R3985 T7143 T7140 prep for,was
R3986 T7144 T7145 det the,kidney
R3987 T7145 T7143 pobj kidney,for
R3988 T7146 T7145 amod developing,kidney
R3989 T7147 T7141 punct : ,were
R3990 T7148 T7149 amod apoptotic,cells
R3991 T7149 T7141 nsubj cells,were
R3992 T7150 T7141 acomp present,were
R3993 T7151 T7141 prep in,were
R3994 T7152 T7153 nmod Ptdsr,embryos
R3995 T7153 T7151 pobj embryos,in
R3996 T7154 T7152 punct +,Ptdsr
R3997 T7155 T7152 punct /,Ptdsr
R3998 T7156 T7152 punct +,Ptdsr
R3999 T7157 T7152 cc and,Ptdsr
R4000 T7158 T7152 conj Ptdsr,Ptdsr
R4001 T7159 T7158 punct -,Ptdsr
R4002 T7160 T7158 punct /,Ptdsr
R4003 T7161 T7158 punct -,Ptdsr
R4004 T7162 T7141 punct ", ",were
R4005 T7163 T7141 prep in,were
R4006 T7164 T7165 amod limited,numbers
R4007 T7165 T7163 pobj numbers,in
R4008 T7166 T7141 punct ", ",were
R4009 T7167 T7141 cc but,were
R4010 T7168 T7169 nsubj we,failed
R4011 T7169 T7141 conj failed,were
R4012 T7170 T7171 aux to,detect
R4013 T7171 T7169 xcomp detect,failed
R4014 T7172 T7173 det any,differences
R4015 T7173 T7171 dobj differences,detect
R4016 T7174 T7173 prep in,differences
R4017 T7175 T7176 det the,number
R4018 T7176 T7174 pobj number,in
R4019 T7177 T7176 prep of,number
R4020 T7178 T7179 amod apoptotic,cells
R4021 T7179 T7177 pobj cells,of
R4022 T7180 T7176 prep between,number
R4023 T7181 T7182 det the,genotypes
R4024 T7182 T7180 pobj genotypes,between
R4025 T7183 T7184 punct (,6g
R4026 T7184 T7169 parataxis 6g,failed
R4027 T7185 T7184 nmod Figure,6g
R4028 T7186 T7184 nmod 6c,6g
R4029 T7187 T7184 punct ",",6g
R4030 T7188 T7184 punct ),6g
R4031 T7189 T7141 punct .,were
R4032 T7191 T7192 advmod Furthermore,was
R4033 T7193 T7192 punct ", ",was
R4034 T7194 T7195 advmod when,continued
R4035 T7195 T7192 advcl continued,was
R4036 T7196 T7195 nsubj we,continued
R4037 T7197 T7198 poss our,analysis
R4038 T7198 T7195 dobj analysis,continued
R4039 T7199 T7198 prep of,analysis
R4040 T7200 T7201 amod apoptotic,cell
R4041 T7201 T7202 compound cell,clearance
R4042 T7202 T7199 pobj clearance,of
R4043 T7203 T7204 advmod in,vivo
R4044 T7204 T7198 advmod vivo,analysis
R4045 T7205 T7195 prep at,continued
R4046 T7206 T7205 pobj E16.5,at
R4047 T7207 T7206 punct ", ",E16.5
R4048 T7208 T7206 conj E17.5,E16.5
R4049 T7209 T7208 cc and,E17.5
R4050 T7210 T7208 conj E18.5,E17.5
R4051 T7211 T7206 prep of,E16.5
R4052 T7212 T7213 amod embryonic,development
R4053 T7213 T7211 pobj development,of
R4054 T7214 T7215 advmod as,as
R4055 T7215 T7205 cc as,at
R4056 T7216 T7215 advmod well,as
R4057 T7217 T7205 conj in,at
R4058 T7218 T7219 amod neonatal,mice
R4059 T7219 T7217 pobj mice,in
R4060 T7220 T7192 punct ", ",was
R4061 T7221 T7222 det the,number
R4062 T7222 T7192 nsubj number,was
R4063 T7223 T7222 cc and,number
R4064 T7224 T7222 conj distribution,number
R4065 T7225 T7222 prep of,number
R4066 T7226 T7227 amod apoptotic,cells
R4067 T7227 T7225 pobj cells,of
R4068 T7228 T7192 acomp similar,was
R4069 T7229 T7192 prep in,was
R4070 T7230 T7231 det both,genotypes
R4071 T7231 T7229 pobj genotypes,in
R4072 T7232 T7192 punct .,was
R4073 T7234 T7235 mark As,observed
R4074 T7235 T7237 advcl observed,showed
R4075 T7236 T7235 advmod already,observed
R4076 T7238 T7235 prep at,observed
R4077 T7239 T7238 pobj E12.5,at
R4078 T7240 T7237 punct ", ",showed
R4079 T7241 T7237 nsubj analysis,showed
R4080 T7242 T7241 prep of,analysis
R4081 T7243 T7244 npadvmod aCasp3,stained
R4082 T7244 T7246 amod stained,sections
R4083 T7245 T7244 punct -,stained
R4084 T7246 T7242 pobj sections,of
R4085 T7247 T7246 prep of,sections
R4086 T7248 T7249 det the,thymus
R4087 T7249 T7247 pobj thymus,of
R4088 T7250 T7249 amod developing,thymus
R4089 T7251 T7249 punct ", ",thymus
R4090 T7252 T7249 conj heart,thymus
R4091 T7253 T7252 punct ", ",heart
R4092 T7254 T7252 conj diaphragm,heart
R4093 T7255 T7254 punct ", ",diaphragm
R4094 T7256 T7257 amod genital,ridge
R4095 T7257 T7254 conj ridge,diaphragm
R4096 T7258 T7257 punct ", ",ridge
R4097 T7259 T7257 conj eyes,ridge
R4098 T7260 T7259 cc and,eyes
R4099 T7261 T7259 conj retina,eyes
R4100 T7262 T7237 advmod convincingly,showed
R4101 T7263 T7264 mark that,was
R4102 T7264 T7237 ccomp was,showed
R4103 T7265 T7264 expl there,was
R4104 T7266 T7267 det no,impairment
R4105 T7267 T7264 attr impairment,was
R4106 T7268 T7267 prep in,impairment
R4107 T7269 T7270 amod apoptotic,removal
R4108 T7270 T7268 pobj removal,in
R4109 T7271 T7270 compound cell,removal
R4110 T7272 T7264 prep in,was
R4111 T7273 T7274 nmod Ptdsr,mice
R4112 T7274 T7272 pobj mice,in
R4113 T7275 T7273 punct -,Ptdsr
R4114 T7276 T7273 punct /,Ptdsr
R4115 T7277 T7273 punct -,Ptdsr
R4116 T7278 T7237 punct .,showed
R4117 T7280 T7281 advmod Moreover,examined
R4118 T7282 T7281 punct ", ",examined
R4119 T7283 T7284 mark because,reported
R4120 T7284 T7281 advcl reported,examined
R4121 T7285 T7284 nsubj Li,reported
R4122 T7286 T7285 cc and,Li
R4123 T7287 T7285 conj colleagues,Li
R4124 T7288 T7289 punct [,31
R4125 T7289 T7287 parataxis 31,colleagues
R4126 T7290 T7289 punct ],31
R4127 T7291 T7292 amod impaired,clearance
R4128 T7292 T7284 dobj clearance,reported
R4129 T7293 T7292 prep of,clearance
R4130 T7294 T7295 amod dead,cells
R4131 T7295 T7293 pobj cells,of
R4132 T7296 T7292 prep during,clearance
R4133 T7297 T7298 compound lung,development
R4134 T7298 T7296 pobj development,during
R4135 T7299 T7292 prep in,clearance
R4136 T7300 T7301 npadvmod Ptdsr,deficient
R4137 T7301 T7303 amod deficient,mice
R4138 T7302 T7301 punct -,deficient
R4139 T7303 T7299 pobj mice,in
R4140 T7304 T7281 punct ", ",examined
R4141 T7305 T7281 nsubj we,examined
R4142 T7306 T7307 det the,rate
R4143 T7307 T7281 dobj rate,examined
R4144 T7308 T7307 prep of,rate
R4145 T7309 T7310 compound apoptosis,induction
R4146 T7310 T7308 pobj induction,of
R4147 T7311 T7310 cc and,induction
R4148 T7312 T7313 compound cell,clearance
R4149 T7313 T7310 conj clearance,induction
R4150 T7314 T7307 prep in,rate
R4151 T7315 T7316 poss our,mice
R4152 T7316 T7314 pobj mice,in
R4153 T7317 T7318 compound Ptdsr,knockout
R4154 T7318 T7316 compound knockout,mice
R4155 T7319 T7318 punct -,knockout
R4156 T7320 T7307 prep in,rate
R4157 T7321 T7322 det the,lung
R4158 T7322 T7320 pobj lung,in
R4159 T7323 T7281 punct .,examined
R4160 T7325 T7326 nsubj Analysis,demonstrated
R4161 T7327 T7325 prep of,Analysis
R4162 T7328 T7329 npadvmod aCasp3,stained
R4163 T7329 T7331 amod stained,tissue
R4164 T7330 T7329 punct -,stained
R4165 T7331 T7327 pobj tissue,of
R4166 T7332 T7331 compound lung,tissue
R4167 T7333 T7325 prep from,Analysis
R4168 T7334 T7335 nmod Ptdsr,mice
R4169 T7335 T7333 pobj mice,from
R4170 T7336 T7334 punct +,Ptdsr
R4171 T7337 T7334 punct /,Ptdsr
R4172 T7338 T7334 punct +,Ptdsr
R4173 T7339 T7334 cc and,Ptdsr
R4174 T7340 T7334 conj Ptdsr,Ptdsr
R4175 T7341 T7340 punct -,Ptdsr
R4176 T7342 T7340 punct /,Ptdsr
R4177 T7343 T7340 punct -,Ptdsr
R4178 T7344 T7335 prep at,mice
R4179 T7345 T7344 pobj E17.5,at
R4180 T7346 T7345 cc and,E17.5
R4181 T7347 T7345 conj P0,E17.5
R4182 T7348 T7349 mark that,was
R4183 T7349 T7326 ccomp was,demonstrated
R4184 T7350 T7349 nsubj apoptosis,was
R4185 T7351 T7352 det an,event
R4186 T7352 T7349 attr event,was
R4187 T7353 T7354 advmod extremely,rare
R4188 T7354 T7352 amod rare,event
R4189 T7355 T7349 prep during,was
R4190 T7356 T7357 compound lung,morphogenesis
R4191 T7357 T7355 pobj morphogenesis,during
R4192 T7358 T7349 prep at,was
R4193 T7359 T7360 det this,stage
R4194 T7360 T7358 pobj stage,at
R4195 T7361 T7326 punct .,demonstrated
R4196 T7363 T7364 prep In,were
R4197 T7365 T7363 pobj addition,In
R4198 T7366 T7364 punct ", ",were
R4199 T7367 T7364 expl there,were
R4200 T7368 T7369 det no,differences
R4201 T7369 T7364 attr differences,were
R4202 T7370 T7369 prep in,differences
R4203 T7371 T7372 det the,number
R4204 T7372 T7370 pobj number,in
R4205 T7373 T7372 cc or,number
R4206 T7374 T7372 conj distribution,number
R4207 T7375 T7372 prep of,number
R4208 T7376 T7377 amod apoptotic,cells
R4209 T7377 T7375 pobj cells,of
R4210 T7378 T7369 prep in,differences
R4211 T7379 T7380 nmod Ptdsr,mice
R4212 T7380 T7378 pobj mice,in
R4213 T7381 T7379 punct -,Ptdsr
R4214 T7382 T7379 punct /,Ptdsr
R4215 T7383 T7379 punct -,Ptdsr
R4216 T7384 T7379 cc and,Ptdsr
R4217 T7385 T7379 conj Ptdsr,Ptdsr
R4218 T7386 T7385 punct +,Ptdsr
R4219 T7387 T7385 punct /,Ptdsr
R4220 T7388 T7385 punct +,Ptdsr
R4221 T7389 T7364 punct .,were
R4222 T7391 T7392 advmod Furthermore,were
R4223 T7393 T7392 punct ", ",were
R4224 T7394 T7392 nsubj we,were
R4225 T7395 T7392 acomp unable,were
R4226 T7396 T7397 aux to,detect
R4227 T7397 T7395 xcomp detect,unable
R4228 T7398 T7399 det any,evidence
R4229 T7399 T7397 dobj evidence,detect
R4230 T7400 T7399 prep of,evidence
R4231 T7401 T7402 compound tissue,necrosis
R4232 T7402 T7400 pobj necrosis,of
R4233 T7403 T7397 prep in,detect
R4234 T7404 T7403 pobj lungs,in
R4235 T7405 T7404 prep from,lungs
R4236 T7406 T7407 npadvmod Ptdsr,deficient
R4237 T7407 T7409 amod deficient,mice
R4238 T7408 T7407 punct -,deficient
R4239 T7409 T7405 pobj mice,from
R4240 T7410 T7392 punct .,were
R4241 T7412 T7413 prep In,observed
R4242 T7414 T7412 pobj contrast,In
R4243 T7415 T7414 prep to,contrast
R4244 T7416 T7417 det the,report
R4245 T7417 T7415 pobj report,to
R4246 T7418 T7417 prep of,report
R4247 T7419 T7418 pobj Li,of
R4248 T7420 T7421 advmod et,al.
R4249 T7421 T7419 advmod al.,Li
R4250 T7422 T7423 punct [,31
R4251 T7423 T7414 parataxis 31,contrast
R4252 T7424 T7423 punct ],31
R4253 T7425 T7413 punct ", ",observed
R4254 T7426 T7413 nsubj we,observed
R4255 T7427 T7413 neg never,observed
R4256 T7428 T7413 dobj recruitment,observed
R4257 T7429 T7428 prep of,recruitment
R4258 T7430 T7429 pobj neutrophils,of
R4259 T7431 T7428 cc or,recruitment
R4260 T7432 T7433 amod other,signs
R4261 T7433 T7428 conj signs,recruitment
R4262 T7434 T7433 prep of,signs
R4263 T7435 T7436 amod pulmonary,inflammation
R4264 T7436 T7434 pobj inflammation,of
R4265 T7437 T7413 prep at,observed
R4266 T7438 T7439 det any,stage
R4267 T7439 T7437 pobj stage,at
R4268 T7440 T7439 prep of,stage
R4269 T7441 T7440 pobj development,of
R4270 T7442 T7413 prep in,observed
R4271 T7443 T7444 poss our,mice
R4272 T7444 T7442 pobj mice,in
R4273 T7445 T7446 npadvmod Ptdsr,deficient
R4274 T7446 T7444 amod deficient,mice
R4275 T7447 T7446 punct -,deficient
R4276 T7448 T7413 punct .,observed
R4277 T7450 T7451 aux To,analyze
R4278 T7451 T7452 advcl analyze,stained
R4279 T7453 T7454 mark whether,recruited
R4280 T7454 T7451 ccomp recruited,analyze
R4281 T7455 T7454 nsubjpass macrophages,recruited
R4282 T7456 T7454 auxpass are,recruited
R4283 T7457 T7454 prep into,recruited
R4284 T7458 T7457 pobj areas,into
R4285 T7459 T7460 advmod where,is
R4286 T7460 T7458 relcl is,areas
R4287 T7461 T7460 nsubj apoptosis,is
R4288 T7462 T7460 acomp prominent,is
R4289 T7463 T7460 prep during,is
R4290 T7464 T7463 pobj embryogenesis,during
R4291 T7465 T7452 punct ", ",stained
R4292 T7466 T7452 nsubj we,stained
R4293 T7467 T7468 amod consecutive,sections
R4294 T7468 T7452 dobj sections,stained
R4295 T7469 T7468 amod serial,sections
R4296 T7470 T7471 preconj either,with
R4297 T7471 T7452 prep with,stained
R4298 T7472 T7473 det the,marker
R4299 T7473 T7471 pobj marker,with
R4300 T7474 T7473 compound macrophage,marker
R4301 T7475 T7473 compound surface,marker
R4302 T7476 T7473 appos F4,marker
R4303 T7477 T7476 punct /,F4
R4304 T7478 T7476 nummod 80,F4
R4305 T7479 T7471 cc or,with
R4306 T7480 T7471 conj with,with
R4307 T7481 T7480 pobj aCasp3,with
R4308 T7482 T7452 punct .,stained
R4309 T7484 T7485 advmod Surprisingly,was
R4310 T7486 T7485 punct ", ",was
R4311 T7487 T7485 expl there,was
R4312 T7488 T7489 det no,co-localization
R4313 T7489 T7485 attr co-localization,was
R4314 T7490 T7489 prep of,co-localization
R4315 T7491 T7490 pobj macrophages,of
R4316 T7492 T7489 prep with,co-localization
R4317 T7493 T7494 amod apoptotic,cells
R4318 T7494 T7492 pobj cells,with
R4319 T7495 T7485 punct .,was
R4320 T7497 T7498 prep In,localized
R4321 T7499 T7500 advmod virtually,tissues
R4322 T7500 T7497 pobj tissues,In
R4323 T7501 T7500 det all,tissues
R4324 T7502 T7500 amod embryonic,tissues
R4325 T7503 T7498 punct ", ",localized
R4326 T7504 T7505 amod apoptotic,cells
R4327 T7505 T7498 nsubjpass cells,localized
R4328 T7506 T7505 cc and,cells
R4329 T7507 T7505 conj macrophages,cells
R4330 T7508 T7498 auxpass were,localized
R4331 T7509 T7498 prep in,localized
R4332 T7510 T7511 amod different,compartments
R4333 T7511 T7509 pobj compartments,in
R4334 T7512 T7513 punct (,6i
R4335 T7513 T7498 parataxis 6i,localized
R4336 T7514 T7513 nmod Figure,6i
R4337 T7515 T7513 nmod 6e,6i
R4338 T7516 T7513 punct ",",6i
R4339 T7517 T7513 punct ;,6i
R4340 T7518 T7513 cc and,6i
R4341 T7519 T7513 conj see,6i
R4342 T7520 T7519 advmod also,see
R4343 T7521 T7522 amod Additional,data
R4344 T7522 T7523 compound data,file
R4345 T7523 T7519 dobj file,see
R4346 T7524 T7523 nummod 1,file
R4347 T7525 T7523 punct ", ",file
R4348 T7526 T7527 compound Figure,S1
R4349 T7527 T7523 npadvmod S1,file
R4350 T7528 T7513 punct ),6i
R4351 T7529 T7498 punct .,localized
R4352 T7531 T7532 nsubj This,suggests
R4353 T7533 T7534 mark that,is
R4354 T7534 T7532 ccomp is,suggests
R4355 T7535 T7534 prep at,is
R4356 T7536 T7537 det this,stage
R4357 T7537 T7535 pobj stage,at
R4358 T7538 T7537 prep of,stage
R4359 T7539 T7538 pobj development,of
R4360 T7540 T7534 nsubj it,is
R4361 T7541 T7534 advmod mainly,is
R4362 T7542 T7543 amod neighboring,cells
R4363 T7543 T7534 attr cells,is
R4364 T7544 T7545 dep that,involved
R4365 T7545 T7543 relcl involved,cells
R4366 T7546 T7545 auxpass are,involved
R4367 T7547 T7545 prep in,involved
R4368 T7548 T7547 pobj removal,in
R4369 T7549 T7548 prep of,removal
R4370 T7550 T7551 amod apoptotic,cells
R4371 T7551 T7549 pobj cells,of
R4372 T7552 T7543 punct ", ",cells
R4373 T7553 T7554 advmod rather,than
R4374 T7554 T7543 cc than,cells
R4375 T7555 T7556 amod professional,macrophages
R4376 T7556 T7543 conj macrophages,cells
R4377 T7557 T7532 punct .,suggests
R4378 T7559 T7560 prep In,reveal
R4379 T7561 T7559 pobj summary,In
R4380 T7562 T7560 punct ", ",reveal
R4381 T7563 T7564 poss our,analysis
R4382 T7564 T7560 nsubj analysis,reveal
R4383 T7565 T7566 advmod in,vivo
R4384 T7566 T7564 advmod vivo,analysis
R4385 T7567 T7560 aux did,reveal
R4386 T7568 T7560 neg not,reveal
R4387 T7569 T7570 det any,impairment
R4388 T7570 T7560 dobj impairment,reveal
R4389 T7571 T7560 prep in,reveal
R4390 T7572 T7573 amod apoptotic,clearance
R4391 T7573 T7571 pobj clearance,in
R4392 T7574 T7573 compound cell,clearance
R4393 T7575 T7560 prep in,reveal
R4394 T7576 T7577 npadvmod Ptdsr,deficient
R4395 T7577 T7579 amod deficient,embryos
R4396 T7578 T7577 punct -,deficient
R4397 T7579 T7575 pobj embryos,in
R4398 T7580 T7560 prep during,reveal
R4399 T7581 T7580 pobj development,during
R4400 T7582 T7560 cc and,reveal
R4401 T7583 T7584 advmod further,suggests
R4402 T7584 T7560 conj suggests,reveal
R4403 T7585 T7586 mark that,mediated
R4404 T7586 T7584 ccomp mediated,suggests
R4405 T7587 T7586 nsubjpass phagocytosis,mediated
R4406 T7588 T7587 prep of,phagocytosis
R4407 T7589 T7590 amod apoptotic,cells
R4408 T7590 T7588 pobj cells,of
R4409 T7591 T7586 auxpass is,mediated
R4410 T7592 T7586 advmod mainly,mediated
R4411 T7593 T7586 prep by,mediated
R4412 T7594 T7595 amod non-professional,cells
R4413 T7595 T7593 pobj cells,by
R4414 T7596 T7595 punct ',cells
R4415 T7597 T7595 nmod bystander,cells
R4416 T7598 T7595 punct ',cells
R4417 T7599 T7560 punct .,reveal
R4418 T7601 T7602 aux To,determine
R4419 T7602 T7603 advcl determine,performed
R4420 T7604 T7605 mark whether,impaired
R4421 T7605 T7602 ccomp impaired,determine
R4422 T7606 T7605 nsubjpass macrophages,impaired
R4423 T7607 T7606 prep from,macrophages
R4424 T7608 T7609 compound Ptdsr,knockout
R4425 T7609 T7611 compound knockout,mice
R4426 T7610 T7609 punct -,knockout
R4427 T7611 T7607 pobj mice,from
R4428 T7612 T7605 auxpass were,impaired
R4429 T7613 T7605 prep in,impaired
R4430 T7614 T7615 det the,efficacy
R4431 T7615 T7613 pobj efficacy,in
R4432 T7616 T7615 prep of,efficacy
R4433 T7617 T7618 amod apoptotic,cell
R4434 T7618 T7619 compound cell,uptake
R4435 T7619 T7616 pobj uptake,of
R4436 T7620 T7621 advmod in,vitro
R4437 T7621 T7615 advmod vitro,efficacy
R4438 T7622 T7603 punct ", ",performed
R4439 T7623 T7603 nsubj we,performed
R4440 T7624 T7625 compound phagocytosis,assays
R4441 T7625 T7603 dobj assays,performed
R4442 T7626 T7603 prep with,performed
R4443 T7627 T7628 amod fetal,liver
R4444 T7628 T7630 npadvmod liver,derived
R4445 T7629 T7628 punct -,liver
R4446 T7630 T7632 amod derived,macrophages
R4447 T7631 T7630 punct -,derived
R4448 T7632 T7626 pobj macrophages,with
R4449 T7633 T7632 punct (,macrophages
R4450 T7634 T7632 appos FLDMs,macrophages
R4451 T7635 T7603 punct ),performed
R4452 T7636 T7603 cc and,performed
R4453 T7637 T7603 conj quantified,performed
R4454 T7638 T7639 poss their,rates
R4455 T7639 T7637 dobj rates,quantified
R4456 T7640 T7639 compound phagocytosis,rates
R4457 T7641 T7603 punct .,performed
R4458 T7643 T7644 nsubjpass Phagocytosis,investigated
R4459 T7645 T7643 prep of,Phagocytosis
R4460 T7646 T7647 amod apoptotic,thymocytes
R4461 T7647 T7645 pobj thymocytes,of
R4462 T7648 T7644 auxpass was,investigated
R4463 T7649 T7644 prep at,investigated
R4464 T7650 T7651 nummod 60,minutes
R4465 T7651 T7649 pobj minutes,at
R4466 T7652 T7650 punct ", ",60
R4467 T7653 T7650 conj 90,60
R4468 T7654 T7653 cc and,90
R4469 T7655 T7653 conj 120,90
R4470 T7656 T7644 prep after,investigated
R4471 T7657 T7656 pobj addition,after
R4472 T7658 T7657 prep of,addition
R4473 T7659 T7660 compound target,cells
R4474 T7660 T7658 pobj cells,of
R4475 T7661 T7644 prep in,investigated
R4476 T7662 T7663 det the,absence
R4477 T7663 T7661 pobj absence,in
R4478 T7664 T7663 prep of,absence
R4479 T7665 T7664 pobj serum,of
R4480 T7666 T7644 punct .,investigated
R4481 T7668 T7669 nsubj Analysis,revealed
R4482 T7670 T7668 prep of,Analysis
R4483 T7671 T7672 compound phagocytosis,rates
R4484 T7672 T7670 pobj rates,of
R4485 T7673 T7668 prep by,Analysis
R4486 T7674 T7675 nmod flow,analysis
R4487 T7675 T7673 pobj analysis,by
R4488 T7676 T7675 amod cytometric,analysis
R4489 T7677 T7675 punct (,analysis
R4490 T7678 T7675 appos FACS,analysis
R4491 T7679 T7669 punct ),revealed
R4492 T7680 T7681 det no,differences
R4493 T7681 T7669 dobj differences,revealed
R4494 T7682 T7681 prep in,differences
R4495 T7683 T7684 det the,efficacy
R4496 T7684 T7682 pobj efficacy,in
R4497 T7685 T7684 prep of,efficacy
R4498 T7686 T7687 amod apoptotic,uptake
R4499 T7687 T7685 pobj uptake,of
R4500 T7688 T7687 compound cell,uptake
R4501 T7689 T7681 prep between,differences
R4502 T7690 T7691 nmod Ptdsr,macrophages
R4503 T7691 T7689 pobj macrophages,between
R4504 T7692 T7690 punct -,Ptdsr
R4505 T7693 T7690 punct /,Ptdsr
R4506 T7694 T7690 punct -,Ptdsr
R4507 T7695 T7690 cc and,Ptdsr
R4508 T7696 T7690 conj Ptdsr,Ptdsr
R4509 T7697 T7696 punct +,Ptdsr
R4510 T7698 T7696 punct /,Ptdsr
R4511 T7699 T7696 punct +,Ptdsr
R4512 T7700 T7669 cc and,revealed
R4513 T7701 T7669 conj demonstrated,revealed
R4514 T7702 T7703 det no,differences
R4515 T7703 T7701 dobj differences,demonstrated
R4516 T7704 T7703 prep in,differences
R4517 T7705 T7706 amod apoptotic,engulfment
R4518 T7706 T7704 pobj engulfment,in
R4519 T7707 T7706 compound cell,engulfment
R4520 T7708 T7703 prep between,differences
R4521 T7709 T7710 amod selected,points
R4522 T7710 T7708 pobj points,between
R4523 T7711 T7710 compound time,points
R4524 T7712 T7713 punct (,shown
R4525 T7713 T7701 parataxis shown,demonstrated
R4526 T7714 T7713 nsubj data,shown
R4527 T7715 T7713 neg not,shown
R4528 T7716 T7713 punct ),shown
R4529 T7717 T7669 punct .,revealed
R4530 T7719 T7720 aux To,re-examine
R4531 T7720 T7721 advcl re-examine,performed
R4532 T7722 T7720 cc and,re-examine
R4533 T7723 T7724 advmod further,validate
R4534 T7724 T7720 conj validate,re-examine
R4535 T7725 T7724 advmod independently,validate
R4536 T7726 T7727 det the,result
R4537 T7727 T7724 dobj result,validate
R4538 T7728 T7727 prep of,result
R4539 T7729 T7730 amod normal,uptake
R4540 T7730 T7728 pobj uptake,of
R4541 T7731 T7732 amod apoptotic,cell
R4542 T7732 T7730 compound cell,uptake
R4543 T7733 T7730 prep by,uptake
R4544 T7734 T7735 nmod Ptdsr,macrophages
R4545 T7735 T7733 pobj macrophages,by
R4546 T7736 T7734 punct -,Ptdsr
R4547 T7737 T7734 punct /,Ptdsr
R4548 T7738 T7734 punct -,Ptdsr
R4549 T7739 T7721 punct ", ",performed
R4550 T7740 T7721 nsubj we,performed
R4551 T7741 T7742 compound phagocytosis,assays
R4552 T7742 T7721 dobj assays,performed
R4553 T7743 T7721 prep for,performed
R4554 T7744 T7745 nummod 60,min
R4555 T7745 T7743 pobj min,for
R4556 T7746 T7721 cc and,performed
R4557 T7747 T7721 conj determined,performed
R4558 T7748 T7749 det the,percentage
R4559 T7749 T7747 dobj percentage,determined
R4560 T7750 T7749 prep of,percentage
R4561 T7751 T7750 pobj macrophages,of
R4562 T7752 T7753 dep that,engulfed
R4563 T7753 T7751 relcl engulfed,macrophages
R4564 T7754 T7753 aux had,engulfed
R4565 T7755 T7756 amod apoptotic,cells
R4566 T7756 T7753 dobj cells,engulfed
R4567 T7757 T7747 punct ", ",determined
R4568 T7758 T7747 prep in,determined
R4569 T7759 T7760 det a,total
R4570 T7760 T7758 pobj total,in
R4571 T7761 T7760 prep of,total
R4572 T7762 T7763 advmod at,300
R4573 T7763 T7765 nummod 300,macrophages
R4574 T7764 T7763 advmod least,300
R4575 T7765 T7761 pobj macrophages,of
R4576 T7766 T7765 acl counted,macrophages
R4577 T7767 T7766 prep by,counted
R4578 T7768 T7769 compound fluorescence,microscopy
R4579 T7769 T7767 pobj microscopy,by
R4580 T7770 T7721 punct .,performed
R4581 T7772 T7773 amod Phagocytosed,cells
R4582 T7773 T7785 nsubjpass cells,identified
R4583 T7774 T7773 punct ", ",cells
R4584 T7775 T7776 nummod 5,carboxytetramethylrhodamine
R4585 T7776 T7778 npadvmod carboxytetramethylrhodamine,labeled
R4586 T7777 T7776 punct -,carboxytetramethylrhodamine
R4587 T7778 T7773 amod labeled,cells
R4588 T7779 T7776 punct -,carboxytetramethylrhodamine
R4589 T7780 T7776 punct (,carboxytetramethylrhodamine
R4590 T7781 T7776 appos TAMRA,carboxytetramethylrhodamine
R4591 T7782 T7776 punct -,carboxytetramethylrhodamine
R4592 T7783 T7776 punct ),carboxytetramethylrhodamine
R4593 T7784 T7773 amod apoptotic,cells
R4594 T7786 T7785 auxpass were,identified
R4595 T7787 T7788 mark as,engulfed
R4596 T7788 T7785 advcl engulfed,identified
R4597 T7789 T7788 auxpass being,engulfed
R4598 T7790 T7788 prep by,engulfed
R4599 T7791 T7790 pobj inclusion,by
R4600 T7856 T7855 amod relative,number
R4601 T7792 T7791 prep in,inclusion
R4602 T7857 T7855 prep of,number
R4603 T7793 T7794 npadvmod F4,labeled
R4604 T7858 T7859 amod phagocytosed,cells
R4605 T7859 T7857 pobj cells,of
R4606 T7860 T7859 amod apoptotic,cells
R4607 T7794 T7798 amod labeled,macrophages
R4608 T7861 T7855 prep per,number
R4609 T7862 T7861 pobj macrophage,per
R4610 T7795 T7793 punct /,F4
R4611 T7863 T7864 punct (,7f
R4612 T7864 T7829 parataxis 7f,found
R4613 T7865 T7866 amod phagocytotic,index
R4614 T7796 T7793 nummod 80,F4
R4615 T7866 T7864 dep index,7f
R4616 T7867 T7864 punct ;,7f
R4617 T7797 T7794 punct -,labeled
R4618 T7868 T7864 compound Figure,7f
R4619 T7869 T7864 punct ),7f
R4620 T7870 T7829 punct .,found
R4621 T7798 T7792 pobj macrophages,in
R4622 T7872 T7873 advmod Moreover,identified
R4623 T7799 T7785 punct .,identified
R4624 T7874 T7873 punct ", ",identified
R4625 T7875 T7876 amod single,macrophages
R4626 T7801 T7802 nsubjpass Analysis,done
R4627 T7876 T7873 nsubjpass macrophages,identified
R4628 T7877 T7876 nmod Ptdsr,macrophages
R4629 T7878 T7877 punct -,Ptdsr
R4630 T7879 T7877 punct /,Ptdsr
R4631 T7803 T7802 auxpass was,done
R4632 T7880 T7877 punct -,Ptdsr
R4633 T7881 T7873 aux could,identified
R4634 T7882 T7873 auxpass be,identified
R4635 T7883 T7884 dep that,engulfed
R4636 T7804 T7802 advmod independently,done
R4637 T7805 T7802 agent by,done
R4638 T7884 T7873 ccomp engulfed,identified
R4639 T7885 T7884 aux had,engulfed
R4640 T7886 T7887 advmod even,more
R4641 T7887 T7888 amod more,cells
R4642 T7806 T7807 nummod three,investigators
R4643 T7888 T7884 dobj cells,engulfed
R4644 T7889 T7888 amod apoptotic,cells
R4645 T7890 T7888 compound target,cells
R4646 T7891 T7892 mark than,had
R4647 T7892 T7888 advcl had,cells
R4648 T7807 T7805 pobj investigators,by
R4649 T7893 T7894 amod wild,type
R4650 T7894 T7896 compound type,macrophages
R4651 T7808 T7809 dep who,were
R4652 T7895 T7894 punct -,type
R4653 T7896 T7892 nsubj macrophages,had
R4654 T7809 T7807 relcl were,investigators
R4655 T7897 T7898 punct (,d
R4656 T7898 T7873 parataxis d,identified
R4657 T7899 T7898 nmod Figure,d
R4658 T7810 T7809 neg not,were
R4659 T7900 T7898 nmod 7b,d
R4660 T7901 T7898 punct ",",d
R4661 T7902 T7898 punct ),d
R4662 T7811 T7809 acomp aware,were
R4663 T7903 T7873 punct .,identified
R4664 T7812 T7811 prep of,aware
R4665 T7905 T7906 advmod Thus,had
R4666 T7907 T7906 punct ", ",had
R4667 T7813 T7814 compound macrophage,genotypes
R4668 T7908 T7909 npadvmod Ptdsr,deficient
R4669 T7909 T7911 amod deficient,macrophages
R4670 T7910 T7909 punct -,deficient
R4671 T7911 T7906 nsubj macrophages,had
R4672 T7814 T7812 pobj genotypes,of
R4673 T7912 T7913 det a,ability
R4674 T7913 T7906 dobj ability,had
R4675 T7815 T7816 punct (,Ptdsr
R4676 T7914 T7913 amod normal,ability
R4677 T7915 T7916 aux to,ingest
R4678 T7916 T7913 acl ingest,ability
R4679 T7917 T7918 amod apoptotic,cells
R4680 T7816 T7814 parataxis Ptdsr,genotypes
R4681 T7918 T7916 dobj cells,ingest
R4682 T7919 T7906 cc and,had
R4683 T7817 T7816 punct -,Ptdsr
R4684 T7920 T7921 auxpass were,impaired
R4685 T7921 T7906 conj impaired,had
R4686 T7922 T7921 neg not,impaired
R4687 T7818 T7816 punct /,Ptdsr
R4688 T7923 T7921 prep in,impaired
R4689 T7924 T7923 pobj recognition,in
R4690 T7925 T7924 cc or,recognition
R4691 T7926 T7924 conj phagocytosis,recognition
R4692 T7927 T7924 prep of,recognition
R4693 T7928 T7927 pobj cells,of
R4694 T7819 T7816 punct -,Ptdsr
R4695 T7929 T7930 dep that,undergone
R4696 T7930 T7928 relcl undergone,cells
R4697 T7820 T7816 cc or,Ptdsr
R4698 T7931 T7930 aux had,undergone
R4699 T7932 T7933 amod programmed,death
R4700 T7933 T7930 dobj death,undergone
R4701 T7821 T7816 conj Ptdsr,Ptdsr
R4702 T7934 T7933 compound cell,death
R4703 T7935 T7906 punct .,had
R4704 T7822 T7821 punct +,Ptdsr
R4705 T7823 T7821 punct /,Ptdsr
R4706 T7824 T7821 punct +,Ptdsr
R4707 T7825 T7816 punct ),Ptdsr
R4708 T7826 T7802 punct .,done
R4709 T7828 T7829 advmod Again,found
R4710 T7830 T7829 punct ", ",found
R4711 T7831 T7832 det no,differences
R4712 T7832 T7829 nsubjpass differences,found
R4713 T7833 T7829 auxpass were,found
R4714 T7834 T7829 prep in,found
R4715 T7835 T7836 det the,percentage
R4716 T7836 T7834 pobj percentage,in
R4717 T7837 T7836 prep of,percentage
R4718 T7838 T7837 pobj macrophages,of
R4719 T7839 T7840 dep that,engulfed
R4720 T7840 T7838 relcl engulfed,macrophages
R4721 T7841 T7840 aux had,engulfed
R4722 T7842 T7843 amod apoptotic,cells
R4723 T7843 T7840 dobj cells,engulfed
R4724 T7844 T7845 punct (,e
R4725 T7845 T7840 parataxis e,engulfed
R4726 T7846 T7845 nmod Figure,e
R4727 T7847 T7845 nmod 7a,e
R4728 T7848 T7845 punct ",",e
R4729 T7849 T7845 nmod c,e
R4730 T7850 T7845 punct ",",e
R4731 T7851 T7845 punct ),e
R4732 T7852 T7834 cc or,in
R4733 T7853 T7834 conj in,in
R4734 T7854 T7855 det the,number
R4735 T7855 T7853 pobj number,in
R4739 T8339 T8340 compound Ptdsr,deficiency
R4740 T8340 T8342 nsubj deficiency,results
R4741 T8341 T8340 punct -,deficiency
R4742 T8343 T8342 prep in,results
R4743 T8344 T8345 amod reduced,production
R4744 T8345 T8343 pobj production,in
R4745 T8346 T8345 prep of,production
R4746 T8347 T8348 amod pro,cytokines
R4747 T8348 T8346 pobj cytokines,of
R4748 T8349 T8347 punct -,pro
R4749 T8350 T8347 cc and,pro
R4750 T8351 T8347 conj anti-inflammatory,pro
R4751 T8352 T8342 prep after,results
R4752 T8353 T8354 compound macrophage,stimulation
R4753 T8354 T8352 pobj stimulation,after
R4754 T8356 T8357 prep In,proposed
R4755 T8358 T8356 pobj addition,In
R4756 T8359 T8358 prep to,addition
R4757 T8360 T8361 poss its,importance
R4758 T8361 T8359 pobj importance,to
R4759 T8362 T8361 amod suggested,importance
R4760 T8363 T8361 prep for,importance
R4761 T8364 T8363 pobj phagocytosis,for
R4762 T8365 T8364 prep of,phagocytosis
R4763 T8366 T8367 amod apoptotic,cells
R4764 T8367 T8365 pobj cells,of
R4765 T8368 T8357 punct ", ",proposed
R4766 T8369 T8357 nsubjpass it,proposed
R4767 T8370 T8357 aux has,proposed
R4768 T8371 T8357 auxpass been,proposed
R4769 T8372 T8373 mark that,fulfils
R4770 T8373 T8357 ccomp fulfils,proposed
R4771 T8374 T8373 nsubj Ptdsr,fulfils
R4772 T8375 T8376 det a,role
R4773 T8376 T8373 dobj role,fulfils
R4774 T8377 T8376 amod second,role
R4775 T8378 T8376 amod crucial,role
R4776 T8379 T8376 prep in,role
R4777 T8380 T8379 pcomp regulating,in
R4778 T8381 T8380 cc and,regulating
R4779 T8382 T8380 conj maintaining,regulating
R4780 T8383 T8384 det a,environment
R4781 T8384 T8382 dobj environment,maintaining
R4782 T8385 T8384 amod non-inflammatory,environment
R4783 T8386 T8382 prep upon,maintaining
R4784 T8387 T8388 det the,recognition
R4785 T8388 T8386 pobj recognition,upon
R4786 T8389 T8388 prep of,recognition
R4787 T8390 T8391 amod apoptotic,cells
R4788 T8391 T8389 pobj cells,of
R4789 T8392 T8388 prep by,recognition
R4790 T8393 T8392 pobj macrophages,by
R4791 T8394 T8395 punct [,26
R4792 T8395 T8357 parataxis 26,proposed
R4793 T8396 T8395 punct ],26
R4794 T8397 T8357 punct .,proposed
R4795 T8399 T8400 nsubj We,tested
R4796 T8401 T8400 advmod therefore,tested
R4797 T8402 T8403 mark whether,were
R4798 T8403 T8400 ccomp were,tested
R4799 T8404 T8405 nmod Ptdsr,macrophages
R4800 T8405 T8403 nsubj macrophages,were
R4801 T8406 T8404 punct -,Ptdsr
R4802 T8407 T8404 punct /,Ptdsr
R4803 T8408 T8404 punct -,Ptdsr
R4804 T8409 T8403 acomp able,were
R4805 T8410 T8411 aux to,release
R4806 T8411 T8409 xcomp release,able
R4807 T8412 T8413 amod anti-inflammatory,cytokines
R4808 T8413 T8411 dobj cytokines,release
R4809 T8414 T8411 prep after,release
R4810 T8415 T8414 pobj ingestion,after
R4811 T8416 T8415 prep of,ingestion
R4812 T8417 T8418 amod apoptotic,cells
R4813 T8418 T8416 pobj cells,of
R4814 T8419 T8400 punct .,tested
R4815 T8421 T8422 nsubj We,examined
R4816 T8423 T8422 dobj levels,examined
R4817 T8424 T8423 prep of,levels
R4818 T8425 T8426 compound TGF,β1
R4819 T8426 T8424 pobj β1,of
R4820 T8427 T8426 punct -,β1
R4821 T8428 T8426 cc and,β1
R4822 T8429 T8426 conj interleukin,β1
R4823 T8430 T8429 punct -,interleukin
R4824 T8431 T8429 nummod 10,interleukin
R4825 T8432 T8433 punct (,IL
R4826 T8433 T8429 parataxis IL,interleukin
R4827 T8434 T8433 punct -,IL
R4828 T8435 T8433 nummod 10,IL
R4829 T8436 T8433 punct ),IL
R4830 T8437 T8422 prep after,examined
R4831 T8438 T8437 pobj stimulation,after
R4832 T8439 T8438 prep of,stimulation
R4833 T8440 T8439 pobj FLDMs,of
R4834 T8441 T8438 prep with,stimulation
R4835 T8442 T8441 pobj lipopolysaccharide,with
R4836 T8443 T8442 punct (,lipopolysaccharide
R4837 T8444 T8442 appos LPS,lipopolysaccharide
R4838 T8445 T8422 punct ),examined
R4839 T8446 T8422 punct ", ",examined
R4840 T8447 T8422 prep with,examined
R4841 T8448 T8447 cc and,with
R4842 T8449 T8447 conj without,with
R4843 T8450 T8449 pobj co-culture,without
R4844 T8451 T8450 prep of,co-culture
R4845 T8452 T8453 amod apoptotic,cells
R4846 T8453 T8451 pobj cells,of
R4847 T8454 T8422 punct .,examined
R4848 T8456 T8457 nsubj Quantification,demonstrated
R4849 T8458 T8456 prep of,Quantification
R4850 T8459 T8460 nmod TGF,β1
R4851 T8460 T8462 nmod β1,levels
R4852 T8461 T8460 punct -,β1
R4853 T8462 T8458 pobj levels,of
R4854 T8463 T8460 cc and,β1
R4855 T8464 T8460 conj IL,β1
R4856 T8465 T8464 punct -,IL
R4857 T8466 T8464 nummod 10,IL
R4858 T8467 T8456 prep after,Quantification
R4859 T8468 T8469 nummod 22,hours
R4860 T8469 T8467 pobj hours,after
R4861 T8470 T8469 prep of,hours
R4862 T8471 T8470 pobj culture,of
R4863 T8472 T8473 mark that,were
R4864 T8473 T8457 ccomp were,demonstrated
R4865 T8474 T8475 nmod Ptdsr,macrophages
R4866 T8475 T8473 nsubj macrophages,were
R4867 T8476 T8474 punct -,Ptdsr
R4868 T8477 T8474 punct /,Ptdsr
R4869 T8478 T8474 punct -,Ptdsr
R4870 T8479 T8473 acomp able,were
R4871 T8480 T8481 aux to,secrete
R4872 T8481 T8479 xcomp secrete,able
R4873 T8482 T8483 det these,cytokines
R4874 T8483 T8481 dobj cytokines,secrete
R4875 T8484 T8483 amod anti-inflammatory,cytokines
R4876 T8485 T8481 prep upon,secrete
R4877 T8486 T8485 pobj ingestion,upon
R4878 T8487 T8486 prep of,ingestion
R4879 T8488 T8489 amod apoptotic,cells
R4880 T8489 T8487 pobj cells,of
R4881 T8490 T8481 punct ", ",secrete
R4882 T8491 T8492 mark although,at
R4883 T8492 T8481 advcl at,secrete
R4884 T8493 T8494 det a,level
R4885 T8494 T8492 pobj level,at
R4886 T8495 T8496 advmod slightly,lower
R4887 T8496 T8494 amod lower,level
R4888 T8497 T8494 prep than,level
R4889 T8498 T8499 amod wild,type
R4890 T8499 T8497 pobj type,than
R4891 T8500 T8499 punct -,type
R4892 T8501 T8502 punct (,b
R4893 T8502 T8457 parataxis b,demonstrated
R4894 T8503 T8502 nmod Figure,b
R4895 T8504 T8502 nmod 8a,b
R4896 T8505 T8502 punct ",",b
R4897 T8506 T8502 punct ),b
R4898 T8507 T8457 punct .,demonstrated
R4899 T8509 T8510 nsubj This,indicates
R4900 T8511 T8512 mark that,compromise
R4901 T8512 T8510 ccomp compromise,indicates
R4902 T8513 T8512 nsubj ablation,compromise
R4903 T8514 T8513 prep of,ablation
R4904 T8515 T8516 compound Ptdsr,function
R4905 T8516 T8514 pobj function,of
R4906 T8517 T8512 aux does,compromise
R4907 T8518 T8512 neg not,compromise
R4908 T8519 T8512 prep in,compromise
R4909 T8520 T8519 amod general,in
R4910 T8521 T8522 det the,ability
R4911 T8522 T8512 dobj ability,compromise
R4912 T8523 T8522 prep of,ability
R4913 T8524 T8523 pobj macrophages,of
R4914 T8525 T8526 aux to,release
R4915 T8526 T8522 acl release,ability
R4916 T8527 T8528 npadvmod immune,suppressive
R4917 T8528 T8530 amod suppressive,cytokines
R4918 T8529 T8528 punct -,suppressive
R4919 T8530 T8526 dobj cytokines,release
R4920 T8531 T8526 prep after,release
R4921 T8532 T8531 pobj recognition,after
R4922 T8533 T8532 cc and,recognition
R4923 T8534 T8532 conj engulfment,recognition
R4924 T8535 T8532 prep of,recognition
R4925 T8536 T8537 amod apoptotic,cells
R4926 T8537 T8535 pobj cells,of
R4927 T8538 T8510 punct .,indicates
R4928 T8540 T8541 aux To,analyze
R4929 T8541 T8542 advcl analyze,stimulated
R4930 T8543 T8544 mark whether,affected
R4931 T8544 T8541 ccomp affected,analyze
R4932 T8545 T8546 amod pro-inflammatory,signaling
R4933 T8546 T8544 nsubjpass signaling,affected
R4934 T8547 T8544 auxpass is,affected
R4935 T8548 T8544 prep in,affected
R4936 T8549 T8550 nmod Ptdsr,macrophages
R4937 T8550 T8548 pobj macrophages,in
R4938 T8551 T8549 punct -,Ptdsr
R4939 T8552 T8549 punct /,Ptdsr
R4940 T8553 T8549 punct -,Ptdsr
R4941 T8554 T8542 punct ", ",stimulated
R4942 T8555 T8542 nsubj we,stimulated
R4943 T8556 T8542 dobj FLDMs,stimulated
R4944 T8557 T8556 prep from,FLDMs
R4945 T8558 T8559 nmod Ptdsr,mice
R4946 T8559 T8557 pobj mice,from
R4947 T8560 T8558 punct +,Ptdsr
R4948 T8561 T8558 punct /,Ptdsr
R4949 T8562 T8558 punct +,Ptdsr
R4950 T8563 T8558 cc and,Ptdsr
R4951 T8564 T8558 conj Ptdsr,Ptdsr
R4952 T8565 T8564 punct -,Ptdsr
R4953 T8566 T8564 punct /,Ptdsr
R4954 T8567 T8564 punct -,Ptdsr
R4955 T8568 T8542 prep with,stimulated
R4956 T8569 T8568 pobj LPS,with
R4957 T8570 T8542 cc and,stimulated
R4958 T8571 T8542 conj measured,stimulated
R4959 T8572 T8571 dobj levels,measured
R4960 T8573 T8572 prep of,levels
R4961 T8574 T8575 compound tumor,α
R4962 T8575 T8573 pobj α,of
R4963 T8576 T8575 compound necrosis,α
R4964 T8577 T8575 compound factor,α
R4965 T8578 T8575 punct -,α
R4966 T8579 T8580 punct (,α
R4967 T8580 T8575 appos α,α
R4968 T8581 T8580 compound TNF,α
R4969 T8582 T8580 punct -,α
R4970 T8583 T8571 punct ),measured
R4971 T8584 T8571 prep at,measured
R4972 T8585 T8586 amod different,points
R4973 T8586 T8584 pobj points,at
R4974 T8587 T8586 compound time,points
R4975 T8588 T8586 prep after,points
R4976 T8589 T8588 pobj stimulation,after
R4977 T8590 T8591 punct (,8c
R4978 T8591 T8571 parataxis 8c,measured
R4979 T8592 T8591 compound Figure,8c
R4980 T8593 T8591 punct ),8c
R4981 T8594 T8542 punct .,stimulated
R4982 T8596 T8597 nmod Ptdsr,macrophages
R4983 T8597 T8601 nsubj macrophages,produced
R4984 T8598 T8596 punct -,Ptdsr
R4985 T8599 T8596 punct /,Ptdsr
R4986 T8600 T8596 punct -,Ptdsr
R4987 T8602 T8603 advmod significantly,less
R4988 T8603 T8604 amod less,α
R4989 T8604 T8601 dobj α,produced
R4990 T8605 T8604 compound TNF,α
R4991 T8606 T8604 punct -,α
R4992 T8607 T8608 mark than,did
R4993 T8608 T8604 advcl did,α
R4994 T8609 T8610 amod wild,type
R4995 T8610 T8612 compound type,macrophages
R4996 T8611 T8610 punct -,type
R4997 T8612 T8608 nsubj macrophages,did
R4998 T8613 T8601 punct .,produced
R4999 T8615 T8616 det The,difference
R5000 T8616 T8617 nsubj difference,was
R5001 T8618 T8616 prep in,difference
R5002 T8619 T8620 compound TNF,α
R5003 T8620 T8622 compound α,secretion
R5004 T8621 T8620 punct -,α
R5005 T8622 T8618 pobj secretion,in
R5006 T8623 T8617 advmod first,was
R5007 T8624 T8617 acomp visible,was
R5008 T8625 T8617 prep after,was
R5009 T8626 T8627 nummod 3,h
R5010 T8627 T8625 pobj h,after
R5011 T8628 T8627 prep of,h
R5012 T8629 T8630 compound LPS,stimulation
R5013 T8630 T8628 pobj stimulation,of
R5014 T8631 T8617 cc and,was
R5015 T8632 T8617 conj became,was
R5016 T8633 T8634 advmod more,prominent
R5017 T8634 T8632 acomp prominent,became
R5018 T8635 T8632 prep during,became
R5019 T8636 T8637 det the,course
R5020 T8637 T8635 pobj course,during
R5021 T8638 T8637 prep of,course
R5022 T8639 T8640 det the,experiment
R5023 T8640 T8638 pobj experiment,of
R5024 T8641 T8642 punct (,8c
R5025 T8642 T8632 parataxis 8c,became
R5026 T8643 T8642 prep for,8c
R5027 T8644 T8643 pobj example,for
R5028 T8645 T8642 punct ", ",8c
R5029 T8646 T8642 prep after,8c
R5030 T8647 T8648 nummod 9,h
R5031 T8648 T8646 pobj h,after
R5032 T8649 T8648 cc and,h
R5033 T8650 T8651 nummod 12,h
R5034 T8651 T8648 conj h,h
R5035 T8652 T8648 prep of,h
R5036 T8653 T8654 compound LPS,stimulation
R5037 T8654 T8652 pobj stimulation,of
R5038 T8655 T8642 punct ;,8c
R5039 T8656 T8642 compound Figure,8c
R5040 T8657 T8642 punct ),8c
R5041 T8658 T8617 punct .,was
R5042 T8660 T8661 aux To,analyze
R5043 T8661 T8662 advcl analyze,stimulated
R5044 T8663 T8664 mark whether,affected
R5045 T8664 T8661 ccomp affected,analyze
R5046 T8665 T8666 compound TNF,α
R5047 T8666 T8668 compound α,release
R5048 T8667 T8666 punct -,α
R5049 T8668 T8664 nsubjpass release,affected
R5050 T8669 T8668 prep by,release
R5051 T8670 T8671 nmod Ptdsr,macrophages
R5052 T8671 T8669 pobj macrophages,by
R5053 T8672 T8670 punct -,Ptdsr
R5054 T8673 T8670 punct /,Ptdsr
R5055 T8674 T8670 punct -,Ptdsr
R5056 T8675 T8664 aux can,affected
R5057 T8676 T8664 auxpass be,affected
R5058 T8677 T8664 prep by,affected
R5059 T8678 T8677 pobj engulfment,by
R5060 T8679 T8678 prep of,engulfment
R5061 T8680 T8681 amod apoptotic,cells
R5062 T8681 T8679 pobj cells,of
R5063 T8682 T8662 punct ", ",stimulated
R5064 T8683 T8662 nsubj we,stimulated
R5065 T8684 T8662 dobj FLDMs,stimulated
R5066 T8685 T8662 prep with,stimulated
R5067 T8686 T8685 pobj LPS,with
R5068 T8687 T8686 punct ", ",LPS
R5069 T8688 T8689 amod apoptotic,cells
R5070 T8689 T8686 conj cells,LPS
R5071 T8690 T8689 cc or,cells
R5072 T8691 T8689 conj both,cells
R5073 T8692 T8662 punct .,stimulated
R5074 T8694 T8695 nsubj Quantification,showed
R5075 T8696 T8694 prep of,Quantification
R5076 T8697 T8698 compound TNF,α
R5077 T8698 T8700 compound α,levels
R5078 T8699 T8698 punct -,α
R5079 T8700 T8696 pobj levels,of
R5080 T8701 T8694 prep by,Quantification
R5081 T8702 T8701 pobj ELISA,by
R5082 T8703 T8694 prep after,Quantification
R5083 T8704 T8705 nummod 22,h
R5084 T8705 T8703 pobj h,after
R5085 T8706 T8707 mark that,release
R5086 T8707 T8695 ccomp release,showed
R5087 T8708 T8709 npadvmod Ptdsr,deficient
R5088 T8709 T8711 amod deficient,macrophages
R5089 T8710 T8709 punct -,deficient
R5090 T8711 T8707 nsubj macrophages,release
R5091 T8712 T8713 amod less,α
R5092 T8713 T8707 dobj α,release
R5093 T8714 T8713 compound TNF,α
R5094 T8715 T8713 punct -,α
R5095 T8716 T8707 prep after,release
R5096 T8717 T8716 pobj stimulation,after
R5097 T8718 T8717 prep with,stimulation
R5098 T8719 T8718 pobj LPS,with
R5099 T8720 T8719 advmod alone,LPS
R5100 T8721 T8716 punct ", ",after
R5101 T8722 T8716 cc and,after
R5102 T8723 T8722 advmod also,and
R5103 T8724 T8716 conj after,after
R5104 T8725 T8726 amod double,stimulation
R5105 T8726 T8724 pobj stimulation,after
R5106 T8727 T8726 prep of,stimulation
R5107 T8728 T8727 pobj macrophages,of
R5108 T8729 T8726 prep with,stimulation
R5109 T8730 T8729 pobj LPS,with
R5110 T8731 T8730 cc and,LPS
R5111 T8732 T8733 amod apoptotic,cells
R5112 T8733 T8730 conj cells,LPS
R5113 T8734 T8735 punct (,8d
R5114 T8735 T8695 parataxis 8d,showed
R5115 T8736 T8735 compound Figure,8d
R5116 T8737 T8735 punct ),8d
R5117 T8738 T8695 punct .,showed
R5118 T8740 T8741 advmod Moreover,demonstrated
R5119 T8742 T8741 punct ", ",demonstrated
R5120 T8743 T8744 det the,stimulation
R5121 T8744 T8741 nsubj stimulation,demonstrated
R5122 T8745 T8744 amod double,stimulation
R5123 T8746 T8747 mark that,inhibited
R5124 T8747 T8741 ccomp inhibited,demonstrated
R5125 T8748 T8749 det the,release
R5126 T8749 T8747 nsubjpass release,inhibited
R5127 T8750 T8751 npadvmod LPS,induced
R5128 T8751 T8749 amod induced,release
R5129 T8752 T8751 punct -,induced
R5130 T8753 T8754 compound TNF,α
R5131 T8754 T8749 compound α,release
R5132 T8755 T8754 punct -,α
R5133 T8756 T8749 prep by,release
R5134 T8757 T8758 nmod Ptdsr,macrophages
R5135 T8758 T8756 pobj macrophages,by
R5136 T8759 T8757 punct -,Ptdsr
R5137 T8760 T8757 punct /,Ptdsr
R5138 T8761 T8757 punct -,Ptdsr
R5139 T8762 T8747 aux could,inhibited
R5140 T8763 T8747 auxpass be,inhibited
R5141 T8764 T8747 prep by,inhibited
R5142 T8765 T8764 pobj co-administration,by
R5143 T8766 T8765 prep of,co-administration
R5144 T8767 T8768 amod apoptotic,cells
R5145 T8768 T8766 pobj cells,of
R5146 T8769 T8747 prep to,inhibited
R5147 T8770 T8771 det an,extent
R5148 T8771 T8769 pobj extent,to
R5149 T8772 T8771 amod comparable,extent
R5150 T8773 T8772 prep to,comparable
R5151 T8774 T8773 pobj that,to
R5152 T8775 T8774 acl seen,that
R5153 T8776 T8775 prep in,seen
R5154 T8777 T8778 amod wild,type
R5155 T8778 T8780 compound type,macrophages
R5156 T8779 T8778 punct -,type
R5157 T8780 T8776 pobj macrophages,in
R5158 T8781 T8741 punct .,demonstrated
R5159 T8783 T8784 amod Similar,results
R5160 T8784 T8785 nsubjpass results,obtained
R5161 T8786 T8785 auxpass were,obtained
R5162 T8787 T8788 advmod when,analyzed
R5163 T8788 T8785 advcl analyzed,obtained
R5164 T8789 T8790 amod other,cytokines
R5165 T8790 T8788 nsubjpass cytokines,analyzed
R5166 T8791 T8790 amod pro-inflammatory,cytokines
R5167 T8792 T8790 punct ", ",cytokines
R5168 T8793 T8794 amod such,as
R5169 T8794 T8790 prep as,cytokines
R5170 T8795 T8794 pobj interleukin,as
R5171 T8796 T8795 punct -,interleukin
R5172 T8797 T8795 nummod 6,interleukin
R5173 T8798 T8795 cc and,interleukin
R5174 T8799 T8800 compound monocyte,protein
R5175 T8800 T8795 conj protein,interleukin
R5176 T8801 T8800 compound chemoattractant,protein
R5177 T8802 T8800 punct -,protein
R5178 T8803 T8800 nummod 1,protein
R5179 T8804 T8788 punct ", ",analyzed
R5180 T8805 T8788 auxpass were,analyzed
R5181 T8806 T8807 punct (,shown
R5182 T8807 T8785 parataxis shown,obtained
R5183 T8808 T8807 nsubj data,shown
R5184 T8809 T8807 neg not,shown
R5185 T8810 T8807 punct ),shown
R5186 T8811 T8785 punct .,obtained
R5187 T8813 T8814 det These,results
R5188 T8814 T8815 nsubj results,indicate
R5189 T8816 T8817 mark that,required
R5190 T8817 T8815 ccomp required,indicate
R5191 T8818 T8817 nsubjpass Ptdsr,required
R5192 T8819 T8817 auxpass is,required
R5193 T8820 T8817 neg not,required
R5194 T8821 T8817 prep in,required
R5195 T8822 T8821 pobj macrophages,in
R5196 T8823 T8817 prep for,required
R5197 T8824 T8825 det the,inhibition
R5198 T8825 T8823 pobj inhibition,for
R5199 T8826 T8825 prep of,inhibition
R5200 T8827 T8828 amod pro-inflammatory,signaling
R5201 T8828 T8826 pobj signaling,of
R5202 T8829 T8817 prep after,required
R5203 T8830 T8829 pobj recognition,after
R5204 T8831 T8830 cc and,recognition
R5205 T8832 T8830 conj engulfment,recognition
R5206 T8833 T8830 prep of,recognition
R5207 T8834 T8835 amod apoptotic,cells
R5208 T8835 T8833 pobj cells,of
R5209 T8836 T8815 punct .,indicate
R5210 T8838 T8839 compound Ptdsr,deficiency
R5211 T8839 T8841 nsubj deficiency,affect
R5212 T8840 T8839 punct -,deficiency
R5213 T8842 T8841 aux does,affect
R5214 T8843 T8841 punct ", ",affect
R5215 T8844 T8841 advmod however,affect
R5216 T8845 T8841 punct ", ",affect
R5217 T8846 T8847 det the,release
R5218 T8847 T8841 dobj release,affect
R5219 T8848 T8847 amod overall,release
R5220 T8849 T8847 prep of,release
R5221 T8850 T8851 amod pro,cytokines
R5222 T8851 T8849 pobj cytokines,of
R5223 T8852 T8850 punct -,pro
R5224 T8853 T8850 cc and,pro
R5225 T8854 T8850 conj anti-inflammatory,pro
R5226 T8855 T8841 prep after,affect
R5227 T8856 T8855 pobj stimulation,after
R5228 T8857 T8856 prep with,stimulation
R5229 T8858 T8857 pobj LPS,with
R5230 T8859 T8855 cc and,after
R5231 T8860 T8855 conj after,after
R5232 T8861 T8862 amod double,treatment
R5233 T8862 T8860 pobj treatment,after
R5234 T8863 T8862 prep with,treatment
R5235 T8864 T8863 pobj LPS,with
R5236 T8865 T8864 cc and,LPS
R5237 T8866 T8867 amod apoptotic,cells
R5238 T8867 T8864 conj cells,LPS
R5239 T8868 T8841 punct ", ",affect
R5240 T8869 T8841 advcl indicating,affect
R5241 T8870 T8871 mark that,have
R5242 T8871 T8869 ccomp have,indicating
R5243 T8872 T8873 npadvmod Ptdsr,deficient
R5244 T8873 T8875 amod deficient,macrophages
R5245 T8874 T8873 punct -,deficient
R5246 T8875 T8871 nsubj macrophages,have
R5247 T8876 T8877 det a,capacity
R5248 T8877 T8871 dobj capacity,have
R5249 T8878 T8877 amod reduced,capacity
R5250 T8879 T8880 aux to,produce
R5251 T8880 T8877 acl produce,capacity
R5252 T8881 T8880 cc or,produce
R5253 T8882 T8880 conj secrete,produce
R5254 T8883 T8884 amod pro,cytokines
R5255 T8884 T8882 dobj cytokines,secrete
R5256 T8885 T8883 punct -,pro
R5257 T8886 T8883 cc and,pro
R5258 T8887 T8883 conj anti-inflammatory,pro
R5259 T8888 T8841 punct .,affect