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PMC:1635039 / 12567-13656 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T6842 0-4 RB denotes Next
T6844 5-7 PRP denotes we
T6843 8-12 VBD denotes used
T6845 13-16 DT denotes the
T6847 17-21 NN denotes R26R
T6848 22-29 NN denotes lineage
T6849 29-30 HYPH denotes -
T6850 30-37 VBG denotes tracing
T6846 38-43 NN denotes assay
T6851 44-46 TO denotes to
T6852 47-56 VB denotes determine
T6853 57-64 IN denotes whether
T6855 65-70 NNS denotes CNCCs
T6856 71-76 MD denotes could
T6857 77-90 RB denotes appropriately
T6854 91-99 VB denotes populate
T6858 100-103 DT denotes the
T6860 104-111 NN denotes outflow
T6861 112-117 NN denotes tract
T6859 118-124 NN denotes region
T6862 124-125 . denotes .
T6863 125-294 sentence denotes Briefly, Alk5Flox/Flox mice were crossed with the ROSA26 Cre reporter mice, and subsequently Alk5Flox/Flox;R26R(+/+) females were crossed with Alk5KO/WT;Wnt1-Cre males.
T6864 126-133 RB denotes Briefly
T6866 133-135 , denotes ,
T6867 135-143 NN denotes Alk5Flox
T6869 143-144 HYPH denotes /
T6868 144-148 NN denotes Flox
T6870 149-153 NNS denotes mice
T6871 154-158 VBD denotes were
T6865 159-166 VBN denotes crossed
T6872 167-171 IN denotes with
T6873 172-175 DT denotes the
T6875 176-182 NN denotes ROSA26
T6877 183-186 NN denotes Cre
T6876 187-195 NN denotes reporter
T6874 196-200 NNS denotes mice
T6878 200-202 , denotes ,
T6879 202-205 CC denotes and
T6880 206-218 RB denotes subsequently
T6882 219-227 NN denotes Alk5Flox
T6884 227-228 HYPH denotes /
T6883 228-232 NN denotes Flox
T6886 232-233 : denotes ;
T6887 233-237 NN denotes R26R
T6888 237-238 -LRB- denotes (
T6889 238-239 SYM denotes +
T6890 239-240 HYPH denotes /
T6885 240-241 SYM denotes +
T6892 241-242 -RRB- denotes )
T6891 243-250 NNS denotes females
T6893 251-255 VBD denotes were
T6881 256-263 VBN denotes crossed
T6894 264-268 IN denotes with
T6895 269-275 NN denotes Alk5KO
T6897 275-276 HYPH denotes /
T6898 276-278 NN denotes WT
T6899 278-279 : denotes ;
T6900 279-283 NN denotes Wnt1
T6901 283-284 HYPH denotes -
T6896 284-287 NN denotes Cre
T6902 288-293 NNS denotes males
T6903 293-294 . denotes .
T6904 294-468 sentence denotes The resulting embryos had the NC-lineage permanently labeled with β-galactosidase expression, and displayed identical phenotypes to those obtained without the R26R reporter.
T6905 295-298 DT denotes The
T6907 299-308 VBG denotes resulting
T6906 309-316 NNS denotes embryos
T6908 317-320 VBD denotes had
T6909 321-324 DT denotes the
T6911 325-327 NN denotes NC
T6912 327-328 HYPH denotes -
T6910 328-335 NN denotes lineage
T6914 336-347 RB denotes permanently
T6913 348-355 VBN denotes labeled
T6915 356-360 IN denotes with
T6916 361-362 NN denotes β
T6918 362-363 HYPH denotes -
T6919 363-376 NN denotes galactosidase
T6917 377-387 NN denotes expression
T6920 387-389 , denotes ,
T6921 389-392 CC denotes and
T6922 393-402 VBD denotes displayed
T6923 403-412 JJ denotes identical
T6924 413-423 NNS denotes phenotypes
T6925 424-426 IN denotes to
T6926 427-432 DT denotes those
T6927 433-441 VBN denotes obtained
T6928 442-449 IN denotes without
T6929 450-453 DT denotes the
T6931 454-458 NN denotes R26R
T6930 459-467 NN denotes reporter
T6932 467-468 . denotes .
T6933 468-620 sentence denotes Staining of embryos for β-galactosidase at E8-E11 did not reveal detectable differences in NCC migration between mutants and controls (data not shown).
T6934 469-477 NN denotes Staining
T6936 478-480 IN denotes of
T6937 481-488 NNS denotes embryos
T6938 489-492 IN denotes for
T6939 493-494 NN denotes β
T6941 494-495 HYPH denotes -
T6940 495-508 NN denotes galactosidase
T6942 509-511 IN denotes at
T6943 512-514 NN denotes E8
T6944 514-515 SYM denotes -
T6945 515-518 NN denotes E11
T6946 519-522 VBD denotes did
T6947 523-526 RB denotes not
T6935 527-533 VB denotes reveal
T6948 534-544 JJ denotes detectable
T6949 545-556 NNS denotes differences
T6950 557-559 IN denotes in
T6951 560-563 NN denotes NCC
T6952 564-573 NN denotes migration
T6953 574-581 IN denotes between
T6954 582-589 NNS denotes mutants
T6955 590-593 CC denotes and
T6956 594-602 NNS denotes controls
T6957 603-604 -LRB- denotes (
T6959 604-608 NNS denotes data
T6960 609-612 RB denotes not
T6958 613-618 VBN denotes shown
T6961 618-619 -RRB- denotes )
T6962 619-620 . denotes .
T6963 620-927 sentence denotes Similarly, serial transverse sectioning of whole mount embryos (E10-E12) and subsequent analysis of positively stained cells in the OFT region demonstrated that CNCCs deficient in Alk5 were capable of populating the PAAs, aortic sac and conotruncal ridges at a level comparable to that of controls (Fig 4).
T6964 621-630 RB denotes Similarly
T6966 630-632 , denotes ,
T6967 632-638 JJ denotes serial
T6969 639-649 JJ denotes transverse
T6968 650-660 NN denotes sectioning
T6970 661-663 IN denotes of
T6971 664-669 JJ denotes whole
T6972 670-675 NN denotes mount
T6973 676-683 NNS denotes embryos
T6974 684-685 -LRB- denotes (
T6975 685-688 NN denotes E10
T6976 688-689 SYM denotes -
T6977 689-692 NN denotes E12
T6978 692-693 -RRB- denotes )
T6979 694-697 CC denotes and
T6980 698-708 JJ denotes subsequent
T6981 709-717 NN denotes analysis
T6982 718-720 IN denotes of
T6983 721-731 RB denotes positively
T6984 732-739 VBN denotes stained
T6985 740-745 NNS denotes cells
T6986 746-748 IN denotes in
T6987 749-752 DT denotes the
T6989 753-756 NN denotes OFT
T6988 757-763 NN denotes region
T6965 764-776 VBD denotes demonstrated
T6990 777-781 IN denotes that
T6992 782-787 NNS denotes CNCCs
T6993 788-797 JJ denotes deficient
T6994 798-800 IN denotes in
T6995 801-805 NN denotes Alk5
T6991 806-810 VBD denotes were
T6996 811-818 JJ denotes capable
T6997 819-821 IN denotes of
T6998 822-832 VBG denotes populating
T6999 833-836 DT denotes the
T7000 837-841 NNS denotes PAAs
T7001 841-843 , denotes ,
T7002 843-849 JJ denotes aortic
T7003 850-853 NN denotes sac
T7004 854-857 CC denotes and
T7005 858-869 JJ denotes conotruncal
T7006 870-876 NNS denotes ridges
T7007 877-879 IN denotes at
T7008 880-881 DT denotes a
T7009 882-887 NN denotes level
T7010 888-898 JJ denotes comparable
T7011 899-901 IN denotes to
T7012 902-906 DT denotes that
T7013 907-909 IN denotes of
T7014 910-918 NNS denotes controls
T7015 919-920 -LRB- denotes (
T7016 920-923 NN denotes Fig
T7017 924-925 CD denotes 4
T7018 925-926 -RRB- denotes )
T7019 926-927 . denotes .
T7020 927-1089 sentence denotes To conclude, the observed phenotypes in Alk5/Wnt1-Cre mutants were certainly not due to defective migration of CNCCs to the pharyngeal and outflow tract regions.
T7021 928-930 TO denotes To
T7022 931-939 VB denotes conclude
T7024 939-941 , denotes ,
T7025 941-944 DT denotes the
T7027 945-953 VBN denotes observed
T7026 954-964 NNS denotes phenotypes
T7028 965-967 IN denotes in
T7029 968-972 NN denotes Alk5
T7031 972-973 HYPH denotes /
T7032 973-977 NN denotes Wnt1
T7033 977-978 HYPH denotes -
T7030 978-981 NN denotes Cre
T7034 982-989 NNS denotes mutants
T7023 990-994 VBD denotes were
T7035 995-1004 RB denotes certainly
T7036 1005-1008 RB denotes not
T7037 1009-1012 IN denotes due
T7038 1013-1015 IN denotes to
T7039 1016-1025 JJ denotes defective
T7040 1026-1035 NN denotes migration
T7041 1036-1038 IN denotes of
T7042 1039-1044 NNS denotes CNCCs
T7043 1045-1047 IN denotes to
T7044 1048-1051 DT denotes the
T7046 1052-1062 JJ denotes pharyngeal
T7047 1063-1066 CC denotes and
T7048 1067-1074 NN denotes outflow
T7049 1075-1080 NN denotes tract
T7045 1081-1088 NNS denotes regions
T7050 1088-1089 . denotes .
R1978 T6842 T6843 advmod Next,used
R1979 T6844 T6843 nsubj we,used
R1980 T6845 T6846 det the,assay
R1981 T6846 T6843 dobj assay,used
R1982 T6847 T6846 nmod R26R,assay
R1983 T6848 T6846 nmod lineage,assay
R1984 T6849 T6848 punct -,lineage
R1985 T6850 T6848 amod tracing,lineage
R1986 T6851 T6852 aux to,determine
R1987 T6852 T6843 advcl determine,used
R1988 T6853 T6854 mark whether,populate
R1989 T6854 T6852 ccomp populate,determine
R1990 T6855 T6854 nsubj CNCCs,populate
R1991 T6856 T6854 aux could,populate
R1992 T6857 T6854 advmod appropriately,populate
R1993 T6858 T6859 det the,region
R1994 T6859 T6854 dobj region,populate
R1995 T6860 T6861 compound outflow,tract
R1996 T6861 T6859 compound tract,region
R1997 T6862 T6843 punct .,used
R1998 T6864 T6865 advmod Briefly,crossed
R1999 T6866 T6865 punct ", ",crossed
R2000 T6867 T6868 compound Alk5Flox,Flox
R2001 T6868 T6870 compound Flox,mice
R2002 T6869 T6868 punct /,Flox
R2003 T6870 T6865 nsubjpass mice,crossed
R2004 T6871 T6865 auxpass were,crossed
R2005 T6872 T6865 prep with,crossed
R2006 T6873 T6874 det the,mice
R2007 T6874 T6872 pobj mice,with
R2008 T6875 T6876 compound ROSA26,reporter
R2009 T6876 T6874 compound reporter,mice
R2010 T6877 T6876 compound Cre,reporter
R2011 T6878 T6865 punct ", ",crossed
R2012 T6879 T6865 cc and,crossed
R2013 T6880 T6881 advmod subsequently,crossed
R2014 T6881 T6865 conj crossed,crossed
R2015 T6882 T6883 nmod Alk5Flox,Flox
R2016 T6883 T6885 nmod Flox,+
R2017 T6884 T6883 punct /,Flox
R2018 T6885 T6891 punct +,females
R2019 T6886 T6885 punct ;,+
R2020 T6887 T6885 nmod R26R,+
R2021 T6888 T6885 punct (,+
R2022 T6889 T6885 punct +,+
R2023 T6890 T6885 punct /,+
R2024 T6891 T6881 nsubjpass females,crossed
R2025 T6892 T6885 punct ),+
R2026 T6893 T6881 auxpass were,crossed
R2027 T6894 T6881 prep with,crossed
R2028 T6895 T6896 nmod Alk5KO,Cre
R2029 T6896 T6902 compound Cre,males
R2030 T6897 T6896 punct /,Cre
R2031 T6898 T6896 nmod WT,Cre
R2032 T6899 T6896 punct ;,Cre
R2033 T6900 T6896 compound Wnt1,Cre
R2034 T6901 T6896 punct -,Cre
R2035 T6902 T6894 pobj males,with
R2036 T6903 T6881 punct .,crossed
R2037 T6905 T6906 det The,embryos
R2038 T6906 T6908 nsubj embryos,had
R2039 T6907 T6906 amod resulting,embryos
R2040 T6909 T6910 det the,lineage
R2041 T6910 T6913 nsubj lineage,labeled
R2042 T6911 T6910 compound NC,lineage
R2043 T6912 T6910 punct -,lineage
R2044 T6913 T6908 ccomp labeled,had
R2045 T6914 T6913 advmod permanently,labeled
R2046 T6915 T6913 prep with,labeled
R2047 T6916 T6917 compound β,expression
R2048 T6917 T6915 pobj expression,with
R2049 T6918 T6917 punct -,expression
R2050 T6919 T6917 compound galactosidase,expression
R2051 T6920 T6908 punct ", ",had
R2052 T6921 T6908 cc and,had
R2053 T6922 T6908 conj displayed,had
R2054 T6923 T6924 amod identical,phenotypes
R2055 T6924 T6922 dobj phenotypes,displayed
R2056 T6925 T6924 prep to,phenotypes
R2057 T6926 T6925 pobj those,to
R2058 T6927 T6926 acl obtained,those
R2059 T6928 T6927 prep without,obtained
R2060 T6929 T6930 det the,reporter
R2061 T6930 T6928 pobj reporter,without
R2062 T6931 T6930 compound R26R,reporter
R2063 T6932 T6908 punct .,had
R2064 T6934 T6935 nsubj Staining,reveal
R2065 T6936 T6934 prep of,Staining
R2066 T6937 T6936 pobj embryos,of
R2067 T6938 T6934 prep for,Staining
R2068 T6939 T6940 compound β,galactosidase
R2069 T6940 T6938 pobj galactosidase,for
R2070 T6941 T6940 punct -,galactosidase
R2071 T6942 T6934 prep at,Staining
R2072 T6943 T6942 pobj E8,at
R2073 T6944 T6945 punct -,E11
R2074 T6945 T6943 prep E11,E8
R2075 T6946 T6935 aux did,reveal
R2076 T6947 T6935 neg not,reveal
R2077 T6948 T6949 amod detectable,differences
R2078 T6949 T6935 dobj differences,reveal
R2079 T6950 T6949 prep in,differences
R2080 T6951 T6952 compound NCC,migration
R2081 T6952 T6950 pobj migration,in
R2082 T6953 T6949 prep between,differences
R2083 T6954 T6953 pobj mutants,between
R2084 T6955 T6954 cc and,mutants
R2085 T6956 T6954 conj controls,mutants
R2086 T6957 T6958 punct (,shown
R2087 T6958 T6935 parataxis shown,reveal
R2088 T6959 T6958 nsubj data,shown
R2089 T6960 T6958 neg not,shown
R2090 T6961 T6958 punct ),shown
R2091 T6962 T6935 punct .,reveal
R2092 T6964 T6965 advmod Similarly,demonstrated
R2093 T6966 T6965 punct ", ",demonstrated
R2094 T6967 T6968 amod serial,sectioning
R2095 T6968 T6965 nsubj sectioning,demonstrated
R2096 T6969 T6968 amod transverse,sectioning
R2097 T6970 T6968 prep of,sectioning
R2098 T6971 T6972 amod whole,mount
R2099 T6972 T6973 compound mount,embryos
R2100 T6973 T6970 pobj embryos,of
R2101 T6974 T6975 punct (,E10
R2102 T6975 T6968 parataxis E10,sectioning
R2103 T6976 T6977 punct -,E12
R2104 T6977 T6975 prep E12,E10
R2105 T6978 T6975 punct ),E10
R2106 T6979 T6968 cc and,sectioning
R2107 T6980 T6981 amod subsequent,analysis
R2108 T6981 T6968 conj analysis,sectioning
R2109 T6982 T6981 prep of,analysis
R2110 T6983 T6984 advmod positively,stained
R2111 T6984 T6985 amod stained,cells
R2112 T6985 T6982 pobj cells,of
R2113 T6986 T6985 prep in,cells
R2114 T6987 T6988 det the,region
R2115 T6988 T6986 pobj region,in
R2116 T6989 T6988 compound OFT,region
R2117 T6990 T6991 mark that,were
R2118 T6991 T6965 ccomp were,demonstrated
R2119 T6992 T6991 nsubj CNCCs,were
R2120 T6993 T6992 amod deficient,CNCCs
R2121 T6994 T6993 prep in,deficient
R2122 T6995 T6994 pobj Alk5,in
R2123 T6996 T6991 acomp capable,were
R2124 T6997 T6996 prep of,capable
R2125 T6998 T6997 pcomp populating,of
R2126 T6999 T7000 det the,PAAs
R2127 T7000 T6998 dobj PAAs,populating
R2128 T7001 T7000 punct ", ",PAAs
R2129 T7002 T7003 amod aortic,sac
R2130 T7003 T7000 conj sac,PAAs
R2131 T7004 T7003 cc and,sac
R2132 T7005 T7006 amod conotruncal,ridges
R2133 T7006 T7003 conj ridges,sac
R2134 T7007 T6998 prep at,populating
R2135 T7008 T7009 det a,level
R2136 T7009 T7007 pobj level,at
R2137 T7010 T7009 amod comparable,level
R2138 T7011 T7010 prep to,comparable
R2139 T7012 T7011 pobj that,to
R2140 T7013 T7012 prep of,that
R2141 T7014 T7013 pobj controls,of
R2142 T7015 T7016 punct (,Fig
R2143 T7016 T6998 parataxis Fig,populating
R2144 T7017 T7016 nummod 4,Fig
R2145 T7018 T7016 punct ),Fig
R2146 T7019 T6965 punct .,demonstrated
R2147 T7021 T7022 aux To,conclude
R2148 T7022 T7023 advcl conclude,were
R2149 T7024 T7023 punct ", ",were
R2150 T7025 T7026 det the,phenotypes
R2151 T7026 T7023 nsubj phenotypes,were
R2152 T7027 T7026 amod observed,phenotypes
R2153 T7028 T7026 prep in,phenotypes
R2154 T7029 T7030 compound Alk5,Cre
R2155 T7030 T7034 compound Cre,mutants
R2156 T7031 T7030 punct /,Cre
R2157 T7032 T7030 compound Wnt1,Cre
R2158 T7033 T7030 punct -,Cre
R2159 T7034 T7028 pobj mutants,in
R2160 T7035 T7023 advmod certainly,were
R2161 T7036 T7023 neg not,were
R2162 T7037 T7023 prep due,were
R2163 T7038 T7037 pcomp to,due
R2164 T7039 T7040 amod defective,migration
R2165 T7040 T7037 pobj migration,due
R2166 T7041 T7040 prep of,migration
R2167 T7042 T7041 pobj CNCCs,of
R2168 T7043 T7040 prep to,migration
R2169 T7044 T7045 det the,regions
R2170 T7045 T7043 pobj regions,to
R2171 T7046 T7045 amod pharyngeal,regions
R2172 T7047 T7046 cc and,pharyngeal
R2173 T7048 T7049 compound outflow,tract
R2174 T7049 T7046 conj tract,pharyngeal
R2175 T7050 T7023 punct .,were