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PMC:1626108 / 43760-44582 JSONTXT 5 Projects

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Id Subject Object Predicate Lexical cue
T8488 0-10 NN denotes Hexokinase
T8490 11-12 CD denotes I
T8489 13-21 NN denotes activity
T8492 22-25 VBD denotes was
T8491 26-36 VBN denotes determined
T8493 37-59 RB denotes spectrophotometrically
T8494 60-62 IN denotes at
T8495 63-65 CD denotes 25
T8496 66-68 NN denotes °C
T8497 69-71 IN denotes by
T8498 72-75 DT denotes the
T8499 76-82 NN denotes method
T8500 83-85 IN denotes of
T8501 86-94 VBG denotes coupling
T8502 95-98 DT denotes the
T8504 99-106 NN denotes glucose
T8506 106-107 HYPH denotes -
T8507 107-108 CD denotes 6
T8508 108-109 HYPH denotes -
T8505 109-118 NN denotes phosphate
T8503 119-129 NN denotes production
T8509 130-133 IN denotes via
T8510 134-141 NN denotes glucose
T8512 141-142 HYPH denotes -
T8513 142-143 CD denotes 6
T8514 143-144 HYPH denotes -
T8511 144-153 NN denotes phosphate
T8515 154-167 NN denotes dehydrogenase
T8516 168-172 IN denotes with
T8517 173-176 DT denotes the
T8518 177-183 NN denotes change
T8519 184-186 IN denotes in
T8520 187-190 DT denotes the
T8521 191-201 NN denotes absorbance
T8522 202-204 IN denotes of
T8523 205-210 NN denotes NADPH
T8524 211-213 IN denotes at
T8525 214-217 CD denotes 340
T8526 218-220 NN denotes nm
T8527 221-224 CC denotes and
T8528 225-227 IN denotes as
T8529 228-237 VBN denotes described
T8530 238-240 IN denotes by
T8531 241-242 -LRB- denotes [
T8532 242-244 CD denotes 66
T8533 244-245 -RRB- denotes ]
T8534 245-246 . denotes .
T8535 246-532 sentence denotes The reaction was started by the addition of purified brain hexokinase I (0.24 μg) (gift from J. Wilson) to 1.0 ml of reaction mixture containing 0.05 M Tris-HCl (pH 8.5), 7.4 mM MgCl2, 6.6 mM ATP, 0.65 mM NADP, 11.1 mM monothioglycerol, and 1 unit of glucose 6-phosphate dehydrogenase.
T8536 247-250 DT denotes The
T8537 251-259 NN denotes reaction
T8539 260-263 VBD denotes was
T8538 264-271 VBN denotes started
T8540 272-274 IN denotes by
T8541 275-278 DT denotes the
T8542 279-287 NN denotes addition
T8543 288-290 IN denotes of
T8544 291-299 VBN denotes purified
T8546 300-305 NN denotes brain
T8545 306-316 NN denotes hexokinase
T8547 317-318 CD denotes I
T8548 319-320 -LRB- denotes (
T8550 320-324 CD denotes 0.24
T8549 325-327 NNS denotes μg
T8551 327-328 -RRB- denotes )
T8552 329-330 -LRB- denotes (
T8553 330-334 NN denotes gift
T8554 335-339 IN denotes from
T8555 340-342 NNP denotes J.
T8556 343-349 NNP denotes Wilson
T8557 349-350 -RRB- denotes )
T8558 351-353 IN denotes to
T8559 354-357 CD denotes 1.0
T8560 358-360 NN denotes ml
T8561 361-363 IN denotes of
T8562 364-372 NN denotes reaction
T8563 373-380 NN denotes mixture
T8564 381-391 VBG denotes containing
T8565 392-396 CD denotes 0.05
T8566 397-398 NNS denotes M
T8568 399-403 NN denotes Tris
T8569 403-404 HYPH denotes -
T8567 404-407 NN denotes HCl
T8570 408-409 -LRB- denotes (
T8571 409-411 NN denotes pH
T8572 412-415 CD denotes 8.5
T8573 415-416 -RRB- denotes )
T8574 416-418 , denotes ,
T8575 418-421 CD denotes 7.4
T8576 422-424 NNS denotes mM
T8577 425-430 NN denotes MgCl2
T8578 430-432 , denotes ,
T8579 432-435 CD denotes 6.6
T8580 436-438 NNS denotes mM
T8581 439-442 NN denotes ATP
T8582 442-444 , denotes ,
T8583 444-448 CD denotes 0.65
T8584 449-451 NNS denotes mM
T8585 452-456 NN denotes NADP
T8586 456-458 , denotes ,
T8587 458-462 CD denotes 11.1
T8588 463-465 NNS denotes mM
T8589 466-482 NN denotes monothioglycerol
T8590 482-484 , denotes ,
T8591 484-487 CC denotes and
T8592 488-489 CD denotes 1
T8593 490-494 NN denotes unit
T8594 495-497 IN denotes of
T8595 498-505 NN denotes glucose
T8597 506-507 CD denotes 6
T8598 507-508 HYPH denotes -
T8596 508-517 NN denotes phosphate
T8599 518-531 NN denotes dehydrogenase
T8600 531-532 . denotes .
T8601 532-725 sentence denotes In the case of hexokinase activity measured in the presence of Cox11 and LD, the purified HKI was incubated with the recombinant proteins for 15 min at 4 °C before measurement of the activity.
T8602 533-535 IN denotes In
T8604 536-539 DT denotes the
T8605 540-544 NN denotes case
T8606 545-547 IN denotes of
T8607 548-558 NN denotes hexokinase
T8608 559-567 NN denotes activity
T8609 568-576 VBN denotes measured
T8610 577-579 IN denotes in
T8611 580-583 DT denotes the
T8612 584-592 NN denotes presence
T8613 593-595 IN denotes of
T8614 596-601 NN denotes Cox11
T8615 602-605 CC denotes and
T8616 606-608 NN denotes LD
T8617 608-610 , denotes ,
T8618 610-613 DT denotes the
T8620 614-622 VBN denotes purified
T8619 623-626 NN denotes HKI
T8621 627-630 VBD denotes was
T8603 631-640 VBN denotes incubated
T8622 641-645 IN denotes with
T8623 646-649 DT denotes the
T8625 650-661 JJ denotes recombinant
T8624 662-670 NN denotes proteins
T8626 671-674 IN denotes for
T8627 675-677 CD denotes 15
T8628 678-681 NNS denotes min
T8629 682-684 IN denotes at
T8630 685-686 CD denotes 4
T8631 687-689 NN denotes °C
T8632 690-696 IN denotes before
T8633 697-708 NN denotes measurement
T8634 709-711 IN denotes of
T8635 712-715 DT denotes the
T8636 716-724 NN denotes activity
T8637 724-725 . denotes .
T8638 725-822 sentence denotes The data were fitted directly into the Michaelis-Menten equation using SIGMAPLOT (SPSS Science).
T8639 726-729 DT denotes The
T8640 730-734 NNS denotes data
T8642 735-739 VBD denotes were
T8641 740-746 VBN denotes fitted
T8643 747-755 RB denotes directly
T8644 756-760 IN denotes into
T8645 761-764 DT denotes the
T8647 765-774 NNP denotes Michaelis
T8649 774-775 HYPH denotes -
T8648 775-781 NNP denotes Menten
T8646 782-790 NN denotes equation
T8650 791-796 VBG denotes using
T8651 797-806 NNP denotes SIGMAPLOT
T8652 807-808 -LRB- denotes (
T8654 808-812 NNP denotes SPSS
T8653 813-820 NNP denotes Science
T8655 820-821 -RRB- denotes )
T8656 821-822 . denotes .
R5565 T8488 T8489 nmod Hexokinase,activity
R5566 T8489 T8491 nsubjpass activity,determined
R5567 T8490 T8488 nummod I,Hexokinase
R5568 T8492 T8491 auxpass was,determined
R5569 T8493 T8491 advmod spectrophotometrically,determined
R5570 T8494 T8491 prep at,determined
R5571 T8495 T8496 nummod 25,°C
R5572 T8496 T8494 pobj °C,at
R5573 T8497 T8491 prep by,determined
R5574 T8498 T8499 det the,method
R5575 T8499 T8497 pobj method,by
R5576 T8500 T8499 prep of,method
R5577 T8501 T8500 pcomp coupling,of
R5578 T8502 T8503 det the,production
R5579 T8503 T8501 dobj production,coupling
R5580 T8504 T8505 nmod glucose,phosphate
R5581 T8505 T8503 compound phosphate,production
R5582 T8506 T8505 punct -,phosphate
R5583 T8507 T8505 nummod 6,phosphate
R5584 T8508 T8505 punct -,phosphate
R5585 T8509 T8503 prep via,production
R5586 T8510 T8511 nmod glucose,phosphate
R5587 T8511 T8515 compound phosphate,dehydrogenase
R5588 T8512 T8511 punct -,phosphate
R5589 T8513 T8511 nummod 6,phosphate
R5590 T8514 T8511 punct -,phosphate
R5591 T8515 T8509 pobj dehydrogenase,via
R5592 T8516 T8501 prep with,coupling
R5593 T8517 T8518 det the,change
R5594 T8518 T8516 pobj change,with
R5595 T8519 T8518 prep in,change
R5596 T8520 T8521 det the,absorbance
R5597 T8521 T8519 pobj absorbance,in
R5598 T8522 T8521 prep of,absorbance
R5599 T8523 T8522 pobj NADPH,of
R5600 T8524 T8518 prep at,change
R5601 T8525 T8526 nummod 340,nm
R5602 T8526 T8524 pobj nm,at
R5603 T8527 T8497 cc and,by
R5604 T8528 T8529 mark as,described
R5605 T8529 T8497 conj described,by
R5606 T8530 T8529 agent by,described
R5607 T8531 T8532 punct [,66
R5608 T8532 T8530 pobj 66,by
R5609 T8533 T8491 punct ],determined
R5610 T8534 T8491 punct .,determined
R5611 T8536 T8537 det The,reaction
R5612 T8537 T8538 nsubjpass reaction,started
R5613 T8539 T8538 auxpass was,started
R5614 T8540 T8538 agent by,started
R5615 T8541 T8542 det the,addition
R5616 T8542 T8540 pobj addition,by
R5617 T8543 T8542 prep of,addition
R5618 T8544 T8545 amod purified,hexokinase
R5619 T8545 T8543 pobj hexokinase,of
R5620 T8546 T8545 compound brain,hexokinase
R5621 T8547 T8545 nummod I,hexokinase
R5622 T8548 T8549 punct (,μg
R5623 T8549 T8545 parataxis μg,hexokinase
R5624 T8550 T8549 nummod 0.24,μg
R5625 T8551 T8549 punct ),μg
R5626 T8552 T8553 punct (,gift
R5627 T8553 T8545 parataxis gift,hexokinase
R5628 T8554 T8553 prep from,gift
R5629 T8555 T8556 compound J.,Wilson
R5630 T8556 T8554 pobj Wilson,from
R5631 T8557 T8553 punct ),gift
R5632 T8558 T8542 prep to,addition
R5633 T8559 T8560 nummod 1.0,ml
R5634 T8560 T8558 pobj ml,to
R5635 T8561 T8560 prep of,ml
R5636 T8562 T8563 compound reaction,mixture
R5637 T8563 T8561 pobj mixture,of
R5638 T8564 T8560 acl containing,ml
R5639 T8565 T8566 nummod 0.05,M
R5640 T8566 T8567 compound M,HCl
R5641 T8567 T8564 dobj HCl,containing
R5642 T8568 T8567 compound Tris,HCl
R5643 T8569 T8567 punct -,HCl
R5644 T8570 T8571 punct (,pH
R5645 T8571 T8567 parataxis pH,HCl
R5646 T8572 T8571 nummod 8.5,pH
R5647 T8573 T8571 punct ),pH
R5648 T8574 T8567 punct ", ",HCl
R5649 T8575 T8576 nummod 7.4,mM
R5650 T8576 T8577 compound mM,MgCl2
R5651 T8577 T8567 conj MgCl2,HCl
R5652 T8578 T8577 punct ", ",MgCl2
R5653 T8579 T8580 nummod 6.6,mM
R5654 T8580 T8581 compound mM,ATP
R5655 T8581 T8577 conj ATP,MgCl2
R5656 T8582 T8581 punct ", ",ATP
R5657 T8583 T8584 nummod 0.65,mM
R5658 T8584 T8585 compound mM,NADP
R5659 T8585 T8581 conj NADP,ATP
R5660 T8586 T8585 punct ", ",NADP
R5661 T8587 T8588 nummod 11.1,mM
R5662 T8588 T8589 compound mM,monothioglycerol
R5663 T8589 T8585 conj monothioglycerol,NADP
R5664 T8590 T8589 punct ", ",monothioglycerol
R5665 T8591 T8589 cc and,monothioglycerol
R5666 T8592 T8593 nummod 1,unit
R5667 T8593 T8589 conj unit,monothioglycerol
R5668 T8594 T8593 prep of,unit
R5669 T8595 T8596 nmod glucose,phosphate
R5670 T8596 T8599 compound phosphate,dehydrogenase
R5671 T8597 T8596 nummod 6,phosphate
R5672 T8598 T8596 punct -,phosphate
R5673 T8599 T8594 pobj dehydrogenase,of
R5674 T8600 T8538 punct .,started
R5675 T8602 T8603 prep In,incubated
R5676 T8604 T8605 det the,case
R5677 T8605 T8602 pobj case,In
R5678 T8606 T8605 prep of,case
R5679 T8607 T8608 compound hexokinase,activity
R5680 T8608 T8606 pobj activity,of
R5681 T8609 T8608 acl measured,activity
R5682 T8610 T8609 prep in,measured
R5683 T8611 T8612 det the,presence
R5684 T8612 T8610 pobj presence,in
R5685 T8613 T8612 prep of,presence
R5686 T8614 T8613 pobj Cox11,of
R5687 T8615 T8614 cc and,Cox11
R5688 T8616 T8614 conj LD,Cox11
R5689 T8617 T8603 punct ", ",incubated
R5690 T8618 T8619 det the,HKI
R5691 T8619 T8603 nsubjpass HKI,incubated
R5692 T8620 T8619 amod purified,HKI
R5693 T8621 T8603 auxpass was,incubated
R5694 T8622 T8603 prep with,incubated
R5695 T8623 T8624 det the,proteins
R5696 T8624 T8622 pobj proteins,with
R5697 T8625 T8624 amod recombinant,proteins
R5698 T8626 T8603 prep for,incubated
R5699 T8627 T8628 nummod 15,min
R5700 T8628 T8626 pobj min,for
R5701 T8629 T8603 prep at,incubated
R5702 T8630 T8631 nummod 4,°C
R5703 T8631 T8629 pobj °C,at
R5704 T8632 T8603 prep before,incubated
R5705 T8633 T8632 pobj measurement,before
R5706 T8634 T8633 prep of,measurement
R5707 T8635 T8636 det the,activity
R5708 T8636 T8634 pobj activity,of
R5709 T8637 T8603 punct .,incubated
R5710 T8639 T8640 det The,data
R5711 T8640 T8641 nsubjpass data,fitted
R5712 T8642 T8641 auxpass were,fitted
R5713 T8643 T8641 advmod directly,fitted
R5714 T8644 T8641 prep into,fitted
R5715 T8645 T8646 det the,equation
R5716 T8646 T8644 pobj equation,into
R5717 T8647 T8648 compound Michaelis,Menten
R5718 T8648 T8646 compound Menten,equation
R5719 T8649 T8648 punct -,Menten
R5720 T8650 T8641 advcl using,fitted
R5721 T8651 T8650 dobj SIGMAPLOT,using
R5722 T8652 T8653 punct (,Science
R5723 T8653 T8651 parataxis Science,SIGMAPLOT
R5724 T8654 T8653 compound SPSS,Science
R5725 T8655 T8653 punct ),Science
R5726 T8656 T8641 punct .,fitted