Id |
Subject |
Object |
Predicate |
Lexical cue |
T7787 |
23-28 |
NN |
denotes |
Yeast |
T7789 |
29-32 |
CD |
denotes |
two |
T7791 |
32-33 |
HYPH |
denotes |
- |
T7790 |
33-39 |
NN |
denotes |
hybrid |
T7788 |
40-49 |
NN |
denotes |
screening |
T7792 |
50-53 |
CC |
denotes |
and |
T7793 |
54-60 |
NNS |
denotes |
assays |
T7794 |
60-61 |
. |
denotes |
. |
T7795 |
61-325 |
sentence |
denotes |
The LD of human RanBP2 (residues 62–711) was subcloned in-frame into the GAL4 DNA-binding domain of bait vector, pBUTE (a kanamycin-resistant version of GAL4 bait vector pGBDUC1) [62] and HybriZAP pBD-GAL4 vector (Stratagene, La Jolla, California, United States). |
T7796 |
62-65 |
DT |
denotes |
The |
T7797 |
66-68 |
NN |
denotes |
LD |
T7799 |
69-71 |
IN |
denotes |
of |
T7800 |
72-77 |
JJ |
denotes |
human |
T7801 |
78-84 |
NN |
denotes |
RanBP2 |
T7802 |
85-86 |
-LRB- |
denotes |
( |
T7804 |
86-94 |
NNS |
denotes |
residues |
T7803 |
95-97 |
CD |
denotes |
62 |
T7805 |
97-98 |
SYM |
denotes |
– |
T7806 |
98-101 |
CD |
denotes |
711 |
T7807 |
101-102 |
-RRB- |
denotes |
) |
T7808 |
103-106 |
VBD |
denotes |
was |
T7798 |
107-116 |
VBN |
denotes |
subcloned |
T7809 |
117-119 |
IN |
denotes |
in |
T7810 |
119-120 |
HYPH |
denotes |
- |
T7811 |
120-125 |
NN |
denotes |
frame |
T7812 |
126-130 |
IN |
denotes |
into |
T7813 |
131-134 |
DT |
denotes |
the |
T7815 |
135-139 |
NN |
denotes |
GAL4 |
T7816 |
140-143 |
NN |
denotes |
DNA |
T7818 |
143-144 |
HYPH |
denotes |
- |
T7817 |
144-151 |
NN |
denotes |
binding |
T7814 |
152-158 |
NN |
denotes |
domain |
T7819 |
159-161 |
IN |
denotes |
of |
T7820 |
162-166 |
NN |
denotes |
bait |
T7821 |
167-173 |
NN |
denotes |
vector |
T7822 |
173-175 |
, |
denotes |
, |
T7823 |
175-180 |
NN |
denotes |
pBUTE |
T7824 |
181-182 |
-LRB- |
denotes |
( |
T7825 |
182-183 |
DT |
denotes |
a |
T7827 |
184-193 |
NN |
denotes |
kanamycin |
T7829 |
193-194 |
HYPH |
denotes |
- |
T7828 |
194-203 |
JJ |
denotes |
resistant |
T7826 |
204-211 |
NN |
denotes |
version |
T7830 |
212-214 |
IN |
denotes |
of |
T7831 |
215-219 |
NN |
denotes |
GAL4 |
T7833 |
220-224 |
NN |
denotes |
bait |
T7832 |
225-231 |
NN |
denotes |
vector |
T7834 |
232-239 |
NN |
denotes |
pGBDUC1 |
T7835 |
239-240 |
-RRB- |
denotes |
) |
T7836 |
241-242 |
-LRB- |
denotes |
[ |
T7837 |
242-244 |
CD |
denotes |
62 |
T7838 |
244-245 |
-RRB- |
denotes |
] |
T7839 |
246-249 |
CC |
denotes |
and |
T7840 |
250-258 |
NN |
denotes |
HybriZAP |
T7842 |
259-262 |
NN |
denotes |
pBD |
T7844 |
262-263 |
HYPH |
denotes |
- |
T7843 |
263-267 |
NN |
denotes |
GAL4 |
T7841 |
268-274 |
NN |
denotes |
vector |
T7845 |
275-276 |
-LRB- |
denotes |
( |
T7846 |
276-286 |
NNP |
denotes |
Stratagene |
T7847 |
286-288 |
, |
denotes |
, |
T7848 |
288-290 |
NNP |
denotes |
La |
T7849 |
291-296 |
NNP |
denotes |
Jolla |
T7850 |
296-298 |
, |
denotes |
, |
T7851 |
298-308 |
NNP |
denotes |
California |
T7852 |
308-310 |
, |
denotes |
, |
T7853 |
310-316 |
NNP |
denotes |
United |
T7854 |
317-323 |
NNP |
denotes |
States |
T7855 |
323-324 |
-RRB- |
denotes |
) |
T7856 |
324-325 |
. |
denotes |
. |
T7857 |
325-534 |
sentence |
denotes |
The former was used to screen ~18 million clones via mating from murine 9- to 10-d-old embryo and brain cDNA libraries at the Molecular Interaction Facility (MIF), University of Wisconsin, Madison, Wisconsin. |
T7858 |
326-329 |
DT |
denotes |
The |
T7859 |
330-336 |
JJ |
denotes |
former |
T7861 |
337-340 |
VBD |
denotes |
was |
T7860 |
341-345 |
VBN |
denotes |
used |
T7862 |
346-348 |
TO |
denotes |
to |
T7863 |
349-355 |
VB |
denotes |
screen |
T7864 |
356-357 |
SYM |
denotes |
~ |
T7866 |
357-359 |
CD |
denotes |
18 |
T7865 |
360-367 |
CD |
denotes |
million |
T7867 |
368-374 |
NNS |
denotes |
clones |
T7868 |
375-378 |
IN |
denotes |
via |
T7869 |
379-385 |
VBG |
denotes |
mating |
T7870 |
386-390 |
IN |
denotes |
from |
T7871 |
391-397 |
NN |
denotes |
murine |
T7873 |
398-399 |
CD |
denotes |
9 |
T7875 |
399-400 |
HYPH |
denotes |
- |
T7876 |
401-403 |
IN |
denotes |
to |
T7874 |
404-406 |
CD |
denotes |
10 |
T7878 |
406-407 |
HYPH |
denotes |
- |
T7877 |
407-408 |
NN |
denotes |
d |
T7880 |
408-409 |
HYPH |
denotes |
- |
T7879 |
409-412 |
JJ |
denotes |
old |
T7881 |
413-419 |
NN |
denotes |
embryo |
T7882 |
420-423 |
CC |
denotes |
and |
T7883 |
424-429 |
NN |
denotes |
brain |
T7884 |
430-434 |
NN |
denotes |
cDNA |
T7872 |
435-444 |
NNS |
denotes |
libraries |
T7885 |
445-447 |
IN |
denotes |
at |
T7886 |
448-451 |
DT |
denotes |
the |
T7888 |
452-461 |
NNP |
denotes |
Molecular |
T7889 |
462-473 |
NNP |
denotes |
Interaction |
T7887 |
474-482 |
NNP |
denotes |
Facility |
T7890 |
483-484 |
-LRB- |
denotes |
( |
T7891 |
484-487 |
NNP |
denotes |
MIF |
T7892 |
487-488 |
-RRB- |
denotes |
) |
T7893 |
488-490 |
, |
denotes |
, |
T7894 |
490-500 |
NNP |
denotes |
University |
T7895 |
501-503 |
IN |
denotes |
of |
T7896 |
504-513 |
NNP |
denotes |
Wisconsin |
T7897 |
513-515 |
, |
denotes |
, |
T7898 |
515-522 |
NNP |
denotes |
Madison |
T7899 |
522-524 |
, |
denotes |
, |
T7900 |
524-533 |
NNP |
denotes |
Wisconsin |
T7901 |
533-534 |
. |
denotes |
. |
T7902 |
534-645 |
sentence |
denotes |
The latter was used to screen via transformation of ~5 million clones from bovine retinal cDNA libraries [32]. |
T7903 |
535-538 |
DT |
denotes |
The |
T7904 |
539-545 |
JJ |
denotes |
latter |
T7906 |
546-549 |
VBD |
denotes |
was |
T7905 |
550-554 |
VBN |
denotes |
used |
T7907 |
555-557 |
TO |
denotes |
to |
T7908 |
558-564 |
VB |
denotes |
screen |
T7909 |
565-568 |
IN |
denotes |
via |
T7910 |
569-583 |
NN |
denotes |
transformation |
T7911 |
584-586 |
IN |
denotes |
of |
T7912 |
587-588 |
SYM |
denotes |
~ |
T7914 |
588-589 |
CD |
denotes |
5 |
T7913 |
590-597 |
CD |
denotes |
million |
T7915 |
598-604 |
NNS |
denotes |
clones |
T7916 |
605-609 |
IN |
denotes |
from |
T7917 |
610-616 |
JJ |
denotes |
bovine |
T7919 |
617-624 |
JJ |
denotes |
retinal |
T7920 |
625-629 |
NN |
denotes |
cDNA |
T7918 |
630-639 |
NNS |
denotes |
libraries |
T7921 |
640-641 |
-LRB- |
denotes |
[ |
T7922 |
641-643 |
CD |
denotes |
32 |
T7923 |
643-644 |
-RRB- |
denotes |
] |
T7924 |
644-645 |
. |
denotes |
. |
T7925 |
645-679 |
sentence |
denotes |
The screens generated six clones. |
T7926 |
646-649 |
DT |
denotes |
The |
T7927 |
650-657 |
NNS |
denotes |
screens |
T7928 |
658-667 |
VBD |
denotes |
generated |
T7929 |
668-671 |
CD |
denotes |
six |
T7930 |
672-678 |
NNS |
denotes |
clones |
T7931 |
678-679 |
. |
denotes |
. |
T7932 |
679-832 |
sentence |
denotes |
One and two in-frame clones were independently isolated from the embryonic and adult brain libraries, respectively, and the interactions were validated. |
T7933 |
680-683 |
CD |
denotes |
One |
T7935 |
684-687 |
CC |
denotes |
and |
T7936 |
688-691 |
CD |
denotes |
two |
T7937 |
692-694 |
IN |
denotes |
in |
T7938 |
694-695 |
HYPH |
denotes |
- |
T7939 |
695-700 |
NN |
denotes |
frame |
T7934 |
701-707 |
NNS |
denotes |
clones |
T7941 |
708-712 |
VBD |
denotes |
were |
T7942 |
713-726 |
RB |
denotes |
independently |
T7940 |
727-735 |
VBN |
denotes |
isolated |
T7943 |
736-740 |
IN |
denotes |
from |
T7944 |
741-744 |
DT |
denotes |
the |
T7946 |
745-754 |
JJ |
denotes |
embryonic |
T7947 |
755-758 |
CC |
denotes |
and |
T7948 |
759-764 |
JJ |
denotes |
adult |
T7949 |
765-770 |
NN |
denotes |
brain |
T7945 |
771-780 |
NNS |
denotes |
libraries |
T7950 |
780-782 |
, |
denotes |
, |
T7951 |
782-794 |
RB |
denotes |
respectively |
T7952 |
794-796 |
, |
denotes |
, |
T7953 |
796-799 |
CC |
denotes |
and |
T7954 |
800-803 |
DT |
denotes |
the |
T7955 |
804-816 |
NNS |
denotes |
interactions |
T7957 |
817-821 |
VBD |
denotes |
were |
T7956 |
822-831 |
VBN |
denotes |
validated |
T7958 |
831-832 |
. |
denotes |
. |
T7959 |
832-864 |
sentence |
denotes |
The three clones encoded Cox11. |
T7960 |
833-836 |
DT |
denotes |
The |
T7962 |
837-842 |
CD |
denotes |
three |
T7961 |
843-849 |
NNS |
denotes |
clones |
T7963 |
850-857 |
VBD |
denotes |
encoded |
T7964 |
858-863 |
NN |
denotes |
Cox11 |
T7965 |
863-864 |
. |
denotes |
. |
T7966 |
864-1046 |
sentence |
denotes |
Interactions between Cox11, LD, and subdomains thereof were quantified by liquid β-galactosidase (Applied Biosystems, Foster City, California, United States) and growth assays [63]. |
T7967 |
865-877 |
NNS |
denotes |
Interactions |
T7969 |
878-885 |
IN |
denotes |
between |
T7970 |
886-891 |
NN |
denotes |
Cox11 |
T7971 |
891-893 |
, |
denotes |
, |
T7972 |
893-895 |
NN |
denotes |
LD |
T7973 |
895-897 |
, |
denotes |
, |
T7974 |
897-900 |
CC |
denotes |
and |
T7975 |
901-911 |
NNS |
denotes |
subdomains |
T7976 |
912-919 |
RB |
denotes |
thereof |
T7977 |
920-924 |
VBD |
denotes |
were |
T7968 |
925-935 |
VBN |
denotes |
quantified |
T7978 |
936-938 |
IN |
denotes |
by |
T7979 |
939-945 |
JJ |
denotes |
liquid |
T7981 |
946-947 |
NN |
denotes |
β |
T7982 |
947-948 |
HYPH |
denotes |
- |
T7980 |
948-961 |
NN |
denotes |
galactosidase |
T7983 |
962-963 |
-LRB- |
denotes |
( |
T7985 |
963-970 |
NNP |
denotes |
Applied |
T7984 |
971-981 |
NNP |
denotes |
Biosystems |
T7986 |
981-983 |
, |
denotes |
, |
T7987 |
983-989 |
NNP |
denotes |
Foster |
T7988 |
990-994 |
NNP |
denotes |
City |
T7989 |
994-996 |
, |
denotes |
, |
T7990 |
996-1006 |
NNP |
denotes |
California |
T7991 |
1006-1008 |
, |
denotes |
, |
T7992 |
1008-1014 |
NNP |
denotes |
United |
T7993 |
1015-1021 |
NNP |
denotes |
States |
T7994 |
1021-1022 |
-RRB- |
denotes |
) |
T7995 |
1023-1026 |
CC |
denotes |
and |
T7996 |
1027-1033 |
NN |
denotes |
growth |
T7997 |
1034-1040 |
NNS |
denotes |
assays |
T7998 |
1041-1042 |
-LRB- |
denotes |
[ |
T7999 |
1042-1044 |
CD |
denotes |
63 |
T8000 |
1044-1045 |
-RRB- |
denotes |
] |
T8001 |
1045-1046 |
. |
denotes |
. |
T8002 |
1046-1260 |
sentence |
denotes |
The maximum specific growth speed (μmax) was determined by calculating μmax = (ln(x t) − ln(x 0))/t, where x t is the OD600 of the culture at t = t, x 0 is the OD600 at t = 0 and t is the time between x 0 and x t. |
T8003 |
1047-1050 |
DT |
denotes |
The |
T8005 |
1051-1058 |
NN |
denotes |
maximum |
T8006 |
1059-1067 |
JJ |
denotes |
specific |
T8007 |
1068-1074 |
NN |
denotes |
growth |
T8004 |
1075-1080 |
NN |
denotes |
speed |
T8009 |
1081-1082 |
-LRB- |
denotes |
( |
T8010 |
1082-1086 |
NN |
denotes |
μmax |
T8011 |
1086-1087 |
-RRB- |
denotes |
) |
T8012 |
1088-1091 |
VBD |
denotes |
was |
T8008 |
1092-1102 |
VBN |
denotes |
determined |
T8013 |
1103-1105 |
IN |
denotes |
by |
T8014 |
1106-1117 |
VBG |
denotes |
calculating |
T8015 |
1118-1122 |
NN |
denotes |
μmax |
T8017 |
1123-1124 |
SYM |
denotes |
= |
T8018 |
1125-1126 |
-LRB- |
denotes |
( |
T8019 |
1126-1128 |
NN |
denotes |
ln |
T8020 |
1128-1129 |
-LRB- |
denotes |
( |
T8016 |
1129-1132 |
NN |
denotes |
x t |
T8021 |
1132-1133 |
-RRB- |
denotes |
) |
T8022 |
1134-1135 |
SYM |
denotes |
− |
T8023 |
1136-1138 |
NN |
denotes |
ln |
T8025 |
1138-1139 |
-LRB- |
denotes |
( |
T8024 |
1139-1142 |
NN |
denotes |
x 0 |
T8026 |
1142-1143 |
-RRB- |
denotes |
) |
T8027 |
1143-1144 |
-RRB- |
denotes |
) |
T8028 |
1144-1145 |
SYM |
denotes |
/ |
T8029 |
1145-1146 |
NN |
denotes |
t |
T8030 |
1146-1148 |
, |
denotes |
, |
T8031 |
1148-1153 |
WRB |
denotes |
where |
T8033 |
1154-1157 |
NN |
denotes |
x t |
T8032 |
1158-1160 |
VBZ |
denotes |
is |
T8034 |
1161-1164 |
DT |
denotes |
the |
T8035 |
1165-1170 |
NN |
denotes |
OD600 |
T8036 |
1171-1173 |
IN |
denotes |
of |
T8037 |
1174-1177 |
DT |
denotes |
the |
T8038 |
1178-1185 |
NN |
denotes |
culture |
T8039 |
1186-1188 |
IN |
denotes |
at |
T8040 |
1189-1190 |
NN |
denotes |
t |
T8042 |
1191-1192 |
SYM |
denotes |
= |
T8041 |
1193-1194 |
NN |
denotes |
t |
T8043 |
1194-1196 |
, |
denotes |
, |
T8044 |
1196-1199 |
NN |
denotes |
x 0 |
T8045 |
1200-1202 |
VBZ |
denotes |
is |
T8046 |
1203-1206 |
DT |
denotes |
the |
T8047 |
1207-1212 |
NN |
denotes |
OD600 |
T8048 |
1213-1215 |
IN |
denotes |
at |
T8049 |
1216-1217 |
NN |
denotes |
t |
T8051 |
1218-1219 |
SYM |
denotes |
= |
T8050 |
1220-1221 |
CD |
denotes |
0 |
T8052 |
1222-1225 |
CC |
denotes |
and |
T8053 |
1226-1227 |
NN |
denotes |
t |
T8054 |
1228-1230 |
VBZ |
denotes |
is |
T8055 |
1231-1234 |
DT |
denotes |
the |
T8056 |
1235-1239 |
NN |
denotes |
time |
T8057 |
1240-1247 |
IN |
denotes |
between |
T8058 |
1248-1251 |
NN |
denotes |
x 0 |
T8059 |
1252-1255 |
CC |
denotes |
and |
T8060 |
1256-1259 |
NN |
denotes |
x t |
T8061 |
1259-1260 |
. |
denotes |
. |
T8062 |
1260-1412 |
sentence |
denotes |
Assays were performed with three independent clones and three samples of each clone, the results were averaged, and the standard deviations calculated. |
T8063 |
1261-1267 |
NNS |
denotes |
Assays |
T8065 |
1268-1272 |
VBD |
denotes |
were |
T8064 |
1273-1282 |
VBN |
denotes |
performed |
T8066 |
1283-1287 |
IN |
denotes |
with |
T8067 |
1288-1293 |
CD |
denotes |
three |
T8069 |
1294-1305 |
JJ |
denotes |
independent |
T8068 |
1306-1312 |
NNS |
denotes |
clones |
T8070 |
1313-1316 |
CC |
denotes |
and |
T8071 |
1317-1322 |
CD |
denotes |
three |
T8072 |
1323-1330 |
NNS |
denotes |
samples |
T8073 |
1331-1333 |
IN |
denotes |
of |
T8074 |
1334-1338 |
DT |
denotes |
each |
T8075 |
1339-1344 |
NN |
denotes |
clone |
T8076 |
1344-1346 |
, |
denotes |
, |
T8077 |
1346-1349 |
DT |
denotes |
the |
T8078 |
1350-1357 |
NNS |
denotes |
results |
T8080 |
1358-1362 |
VBD |
denotes |
were |
T8079 |
1363-1371 |
VBN |
denotes |
averaged |
T8081 |
1371-1373 |
, |
denotes |
, |
T8082 |
1373-1376 |
CC |
denotes |
and |
T8083 |
1377-1380 |
DT |
denotes |
the |
T8085 |
1381-1389 |
JJ |
denotes |
standard |
T8084 |
1390-1400 |
NNS |
denotes |
deviations |
T8086 |
1401-1411 |
VBN |
denotes |
calculated |
T8087 |
1411-1412 |
. |
denotes |
. |
T8119 |
1414-1418 |
NN |
denotes |
Site |
T8121 |
1418-1419 |
HYPH |
denotes |
- |
T8120 |
1419-1427 |
VBN |
denotes |
directed |
T8123 |
1428-1431 |
CC |
denotes |
and |
T8124 |
1432-1440 |
NN |
denotes |
deletion |
T8122 |
1441-1452 |
NN |
denotes |
mutagenesis |
T8125 |
1453-1456 |
CC |
denotes |
and |
T8126 |
1457-1464 |
NN |
denotes |
plasmid |
T8127 |
1465-1477 |
NN |
denotes |
construction |
T8128 |
1477-1478 |
. |
denotes |
. |
T8129 |
1478-1598 |
sentence |
denotes |
Deletion mutagenesis was carried out with pairs of primers against domains of interest described in the figure legends. |
T8130 |
1479-1487 |
NN |
denotes |
Deletion |
T8131 |
1488-1499 |
NN |
denotes |
mutagenesis |
T8133 |
1500-1503 |
VBD |
denotes |
was |
T8132 |
1504-1511 |
VBN |
denotes |
carried |
T8134 |
1512-1515 |
RP |
denotes |
out |
T8135 |
1516-1520 |
IN |
denotes |
with |
T8136 |
1521-1526 |
NNS |
denotes |
pairs |
T8137 |
1527-1529 |
IN |
denotes |
of |
T8138 |
1530-1537 |
NNS |
denotes |
primers |
T8139 |
1538-1545 |
IN |
denotes |
against |
T8140 |
1546-1553 |
NNS |
denotes |
domains |
T8141 |
1554-1556 |
IN |
denotes |
of |
T8142 |
1557-1565 |
NN |
denotes |
interest |
T8143 |
1566-1575 |
VBN |
denotes |
described |
T8144 |
1576-1578 |
IN |
denotes |
in |
T8145 |
1579-1582 |
DT |
denotes |
the |
T8147 |
1583-1589 |
NN |
denotes |
figure |
T8146 |
1590-1597 |
NNS |
denotes |
legends |
T8148 |
1597-1598 |
. |
denotes |
. |
T8149 |
1598-1708 |
sentence |
denotes |
PCR products were subcloned into pGEX-KG [64], HybriZAP pBD-GAL4 vector (Stratagene), and pBUTE [62] vectors. |
T8150 |
1599-1602 |
NN |
denotes |
PCR |
T8151 |
1603-1611 |
NNS |
denotes |
products |
T8153 |
1612-1616 |
VBD |
denotes |
were |
T8152 |
1617-1626 |
VBN |
denotes |
subcloned |
T8154 |
1627-1631 |
IN |
denotes |
into |
T8155 |
1632-1636 |
NN |
denotes |
pGEX |
T8157 |
1636-1637 |
HYPH |
denotes |
- |
T8156 |
1637-1639 |
NN |
denotes |
KG |
T8158 |
1640-1641 |
-LRB- |
denotes |
[ |
T8159 |
1641-1643 |
CD |
denotes |
64 |
T8160 |
1643-1644 |
-RRB- |
denotes |
] |
T8161 |
1644-1646 |
, |
denotes |
, |
T8162 |
1646-1654 |
NN |
denotes |
HybriZAP |
T8164 |
1655-1658 |
NN |
denotes |
pBD |
T8166 |
1658-1659 |
HYPH |
denotes |
- |
T8165 |
1659-1663 |
NN |
denotes |
GAL4 |
T8163 |
1664-1670 |
NN |
denotes |
vector |
T8167 |
1671-1672 |
-LRB- |
denotes |
( |
T8168 |
1672-1682 |
NNP |
denotes |
Stratagene |
T8169 |
1682-1683 |
-RRB- |
denotes |
) |
T8170 |
1683-1685 |
, |
denotes |
, |
T8171 |
1685-1688 |
CC |
denotes |
and |
T8172 |
1689-1694 |
NN |
denotes |
pBUTE |
T8174 |
1695-1696 |
-LRB- |
denotes |
[ |
T8175 |
1696-1698 |
CD |
denotes |
62 |
T8176 |
1698-1699 |
-RRB- |
denotes |
] |
T8173 |
1700-1707 |
NNS |
denotes |
vectors |
T8177 |
1707-1708 |
. |
denotes |
. |
T8178 |
1708-1768 |
sentence |
denotes |
GST-LDZIP alone comprised residues 447–483 of human RanBP2. |
T8179 |
1709-1712 |
NN |
denotes |
GST |
T8181 |
1712-1713 |
HYPH |
denotes |
- |
T8180 |
1713-1718 |
NN |
denotes |
LDZIP |
T8183 |
1719-1724 |
RB |
denotes |
alone |
T8182 |
1725-1734 |
VBN |
denotes |
comprised |
T8184 |
1735-1743 |
NNS |
denotes |
residues |
T8185 |
1744-1747 |
CD |
denotes |
447 |
T8186 |
1747-1748 |
SYM |
denotes |
– |
T8187 |
1748-1751 |
CD |
denotes |
483 |
T8188 |
1752-1754 |
IN |
denotes |
of |
T8189 |
1755-1760 |
JJ |
denotes |
human |
T8190 |
1761-1767 |
NN |
denotes |
RanBP2 |
T8191 |
1767-1768 |
. |
denotes |
. |
T8257 |
1770-1773 |
NN |
denotes |
GST |
T8259 |
1774-1778 |
VB |
denotes |
pull |
T8260 |
1778-1779 |
HYPH |
denotes |
- |
T8261 |
1779-1783 |
RP |
denotes |
down |
T8262 |
1784-1787 |
CC |
denotes |
and |
T8263 |
1788-1807 |
NN |
denotes |
immunoprecipitation |
T8258 |
1808-1814 |
NNS |
denotes |
assays |
T8264 |
1814-1815 |
. |
denotes |
. |
T8265 |
1815-1948 |
sentence |
denotes |
CHAPS-solubilized retinal extracts, expression, and purification of GST-fused constructs were prepared as previously described [65]. |
T8266 |
1816-1821 |
NN |
denotes |
CHAPS |
T8268 |
1821-1822 |
HYPH |
denotes |
- |
T8267 |
1822-1833 |
VBN |
denotes |
solubilized |
T8270 |
1834-1841 |
JJ |
denotes |
retinal |
T8269 |
1842-1850 |
NNS |
denotes |
extracts |
T8272 |
1850-1852 |
, |
denotes |
, |
T8273 |
1852-1862 |
NN |
denotes |
expression |
T8274 |
1862-1864 |
, |
denotes |
, |
T8275 |
1864-1867 |
CC |
denotes |
and |
T8276 |
1868-1880 |
NN |
denotes |
purification |
T8277 |
1881-1883 |
IN |
denotes |
of |
T8278 |
1884-1887 |
NN |
denotes |
GST |
T8280 |
1887-1888 |
HYPH |
denotes |
- |
T8279 |
1888-1893 |
VBN |
denotes |
fused |
T8281 |
1894-1904 |
NNS |
denotes |
constructs |
T8282 |
1905-1909 |
VBD |
denotes |
were |
T8271 |
1910-1918 |
VBN |
denotes |
prepared |
T8283 |
1919-1921 |
IN |
denotes |
as |
T8285 |
1922-1932 |
RB |
denotes |
previously |
T8284 |
1933-1942 |
VBN |
denotes |
described |
T8286 |
1943-1944 |
-LRB- |
denotes |
[ |
T8287 |
1944-1946 |
CD |
denotes |
65 |
T8288 |
1946-1947 |
-RRB- |
denotes |
] |
T8289 |
1947-1948 |
. |
denotes |
. |
T8290 |
1948-2030 |
sentence |
denotes |
GST pull-down assays were carried out with 0.5–2.2 μM of GST-fused proteins [65]. |
T8291 |
1949-1952 |
NN |
denotes |
GST |
T8293 |
1953-1957 |
VB |
denotes |
pull |
T8294 |
1957-1958 |
HYPH |
denotes |
- |
T8295 |
1958-1962 |
RP |
denotes |
down |
T8292 |
1963-1969 |
NNS |
denotes |
assays |
T8297 |
1970-1974 |
VBD |
denotes |
were |
T8296 |
1975-1982 |
VBN |
denotes |
carried |
T8298 |
1983-1986 |
RP |
denotes |
out |
T8299 |
1987-1991 |
IN |
denotes |
with |
T8300 |
1992-1995 |
CD |
denotes |
0.5 |
T8302 |
1995-1996 |
SYM |
denotes |
– |
T8301 |
1996-1999 |
CD |
denotes |
2.2 |
T8303 |
2000-2002 |
NNS |
denotes |
μM |
T8304 |
2003-2005 |
IN |
denotes |
of |
T8305 |
2006-2009 |
NN |
denotes |
GST |
T8307 |
2009-2010 |
HYPH |
denotes |
- |
T8306 |
2010-2015 |
VBN |
denotes |
fused |
T8308 |
2016-2024 |
NN |
denotes |
proteins |
T8309 |
2025-2026 |
-LRB- |
denotes |
[ |
T8310 |
2026-2028 |
CD |
denotes |
65 |
T8311 |
2028-2029 |
-RRB- |
denotes |
] |
T8312 |
2029-2030 |
. |
denotes |
. |
T8313 |
2030-2151 |
sentence |
denotes |
Co-precipitates were resolved on SDS-PAGE and analyzed by Western blot with antibodies described in the Results section. |
T8314 |
2031-2046 |
NNS |
denotes |
Co-precipitates |
T8316 |
2047-2051 |
VBD |
denotes |
were |
T8315 |
2052-2060 |
VBN |
denotes |
resolved |
T8317 |
2061-2063 |
IN |
denotes |
on |
T8318 |
2064-2067 |
NN |
denotes |
SDS |
T8320 |
2067-2068 |
HYPH |
denotes |
- |
T8319 |
2068-2072 |
NN |
denotes |
PAGE |
T8321 |
2073-2076 |
CC |
denotes |
and |
T8322 |
2077-2085 |
VBN |
denotes |
analyzed |
T8323 |
2086-2088 |
IN |
denotes |
by |
T8324 |
2089-2096 |
NNP |
denotes |
Western |
T8325 |
2097-2101 |
NN |
denotes |
blot |
T8326 |
2102-2106 |
IN |
denotes |
with |
T8327 |
2107-2117 |
NNS |
denotes |
antibodies |
T8328 |
2118-2127 |
VBN |
denotes |
described |
T8329 |
2128-2130 |
IN |
denotes |
in |
T8330 |
2131-2134 |
DT |
denotes |
the |
T8332 |
2135-2142 |
NNS |
denotes |
Results |
T8331 |
2143-2150 |
NN |
denotes |
section |
T8333 |
2150-2151 |
. |
denotes |
. |
T8334 |
2151-2323 |
sentence |
denotes |
Unfolded and partially denatured Cox11 were generated by incubating recombinant and native Cox11 overnight with 5.6 and 7 M guanidine hydrochloride and urea, respectively. |
T8335 |
2152-2160 |
VBN |
denotes |
Unfolded |
T8337 |
2161-2164 |
CC |
denotes |
and |
T8338 |
2165-2174 |
RB |
denotes |
partially |
T8339 |
2175-2184 |
VBN |
denotes |
denatured |
T8336 |
2185-2190 |
NN |
denotes |
Cox11 |
T8341 |
2191-2195 |
VBD |
denotes |
were |
T8340 |
2196-2205 |
VBN |
denotes |
generated |
T8342 |
2206-2208 |
IN |
denotes |
by |
T8343 |
2209-2219 |
VBG |
denotes |
incubating |
T8344 |
2220-2231 |
JJ |
denotes |
recombinant |
T8346 |
2232-2235 |
CC |
denotes |
and |
T8347 |
2236-2242 |
JJ |
denotes |
native |
T8345 |
2243-2248 |
NN |
denotes |
Cox11 |
T8348 |
2249-2258 |
RB |
denotes |
overnight |
T8349 |
2259-2263 |
IN |
denotes |
with |
T8350 |
2264-2267 |
CD |
denotes |
5.6 |
T8352 |
2268-2271 |
CC |
denotes |
and |
T8353 |
2272-2273 |
CD |
denotes |
7 |
T8351 |
2274-2275 |
NNS |
denotes |
M |
T8354 |
2276-2285 |
NN |
denotes |
guanidine |
T8355 |
2286-2299 |
NN |
denotes |
hydrochloride |
T8356 |
2300-2303 |
CC |
denotes |
and |
T8357 |
2304-2308 |
NN |
denotes |
urea |
T8358 |
2308-2310 |
, |
denotes |
, |
T8359 |
2310-2322 |
RB |
denotes |
respectively |
T8360 |
2322-2323 |
. |
denotes |
. |
T8361 |
2323-2414 |
sentence |
denotes |
The Cox11 conformers were then diluted ~20-fold in CHAPS-binding buffer containing GST-LD. |
T8362 |
2324-2327 |
DT |
denotes |
The |
T8364 |
2328-2333 |
NN |
denotes |
Cox11 |
T8363 |
2334-2344 |
NNS |
denotes |
conformers |
T8366 |
2345-2349 |
VBD |
denotes |
were |
T8367 |
2350-2354 |
RB |
denotes |
then |
T8365 |
2355-2362 |
VBN |
denotes |
diluted |
T8368 |
2363-2364 |
SYM |
denotes |
~ |
T8369 |
2364-2371 |
RB |
denotes |
20-fold |
T8370 |
2372-2374 |
IN |
denotes |
in |
T8371 |
2375-2380 |
NN |
denotes |
CHAPS |
T8373 |
2380-2381 |
HYPH |
denotes |
- |
T8372 |
2381-2388 |
NN |
denotes |
binding |
T8374 |
2389-2395 |
NN |
denotes |
buffer |
T8375 |
2396-2406 |
VBG |
denotes |
containing |
T8376 |
2407-2410 |
NN |
denotes |
GST |
T8378 |
2410-2411 |
HYPH |
denotes |
- |
T8377 |
2411-2413 |
NN |
denotes |
LD |
T8379 |
2413-2414 |
. |
denotes |
. |
T8380 |
2414-2564 |
sentence |
denotes |
Immunoprecipitation assays with 5 μg of antibody and Western blots (~ 200–400 ng/ml of antibody) were performed exactly as described previously [13]. |
T8381 |
2415-2434 |
NN |
denotes |
Immunoprecipitation |
T8382 |
2435-2441 |
NNS |
denotes |
assays |
T8384 |
2442-2446 |
IN |
denotes |
with |
T8385 |
2447-2448 |
CD |
denotes |
5 |
T8386 |
2449-2451 |
NNS |
denotes |
μg |
T8387 |
2452-2454 |
IN |
denotes |
of |
T8388 |
2455-2463 |
NN |
denotes |
antibody |
T8389 |
2464-2467 |
CC |
denotes |
and |
T8390 |
2468-2475 |
NNP |
denotes |
Western |
T8391 |
2476-2481 |
NNS |
denotes |
blots |
T8392 |
2482-2483 |
-LRB- |
denotes |
( |
T8394 |
2483-2484 |
SYM |
denotes |
~ |
T8396 |
2485-2488 |
CD |
denotes |
200 |
T8397 |
2488-2489 |
SYM |
denotes |
– |
T8395 |
2489-2492 |
CD |
denotes |
400 |
T8393 |
2493-2495 |
NN |
denotes |
ng |
T8398 |
2495-2496 |
SYM |
denotes |
/ |
T8399 |
2496-2498 |
NN |
denotes |
ml |
T8400 |
2499-2501 |
IN |
denotes |
of |
T8401 |
2502-2510 |
NN |
denotes |
antibody |
T8402 |
2510-2511 |
-RRB- |
denotes |
) |
T8403 |
2512-2516 |
VBD |
denotes |
were |
T8383 |
2517-2526 |
VBN |
denotes |
performed |
T8404 |
2527-2534 |
RB |
denotes |
exactly |
T8406 |
2535-2537 |
IN |
denotes |
as |
T8405 |
2538-2547 |
VBN |
denotes |
described |
T8407 |
2548-2558 |
RB |
denotes |
previously |
T8408 |
2559-2560 |
-LRB- |
denotes |
[ |
T8409 |
2560-2562 |
CD |
denotes |
13 |
T8410 |
2562-2563 |
-RRB- |
denotes |
] |
T8411 |
2563-2564 |
. |
denotes |
. |
T8483 |
2566-2576 |
NN |
denotes |
Hexokinase |
T8485 |
2577-2578 |
CD |
denotes |
I |
T8484 |
2579-2584 |
NN |
denotes |
assay |
T8486 |
2584-2585 |
. |
denotes |
. |
T8487 |
2585-2832 |
sentence |
denotes |
Hexokinase I activity was determined spectrophotometrically at 25 °C by the method of coupling the glucose-6-phosphate production via glucose-6-phosphate dehydrogenase with the change in the absorbance of NADPH at 340 nm and as described by [66]. |
T8488 |
2586-2596 |
NN |
denotes |
Hexokinase |
T8490 |
2597-2598 |
CD |
denotes |
I |
T8489 |
2599-2607 |
NN |
denotes |
activity |
T8492 |
2608-2611 |
VBD |
denotes |
was |
T8491 |
2612-2622 |
VBN |
denotes |
determined |
T8493 |
2623-2645 |
RB |
denotes |
spectrophotometrically |
T8494 |
2646-2648 |
IN |
denotes |
at |
T8495 |
2649-2651 |
CD |
denotes |
25 |
T8496 |
2652-2654 |
NN |
denotes |
°C |
T8497 |
2655-2657 |
IN |
denotes |
by |
T8498 |
2658-2661 |
DT |
denotes |
the |
T8499 |
2662-2668 |
NN |
denotes |
method |
T8500 |
2669-2671 |
IN |
denotes |
of |
T8501 |
2672-2680 |
VBG |
denotes |
coupling |
T8502 |
2681-2684 |
DT |
denotes |
the |
T8504 |
2685-2692 |
NN |
denotes |
glucose |
T8506 |
2692-2693 |
HYPH |
denotes |
- |
T8507 |
2693-2694 |
CD |
denotes |
6 |
T8508 |
2694-2695 |
HYPH |
denotes |
- |
T8505 |
2695-2704 |
NN |
denotes |
phosphate |
T8503 |
2705-2715 |
NN |
denotes |
production |
T8509 |
2716-2719 |
IN |
denotes |
via |
T8510 |
2720-2727 |
NN |
denotes |
glucose |
T8512 |
2727-2728 |
HYPH |
denotes |
- |
T8513 |
2728-2729 |
CD |
denotes |
6 |
T8514 |
2729-2730 |
HYPH |
denotes |
- |
T8511 |
2730-2739 |
NN |
denotes |
phosphate |
T8515 |
2740-2753 |
NN |
denotes |
dehydrogenase |
T8516 |
2754-2758 |
IN |
denotes |
with |
T8517 |
2759-2762 |
DT |
denotes |
the |
T8518 |
2763-2769 |
NN |
denotes |
change |
T8519 |
2770-2772 |
IN |
denotes |
in |
T8520 |
2773-2776 |
DT |
denotes |
the |
T8521 |
2777-2787 |
NN |
denotes |
absorbance |
T8522 |
2788-2790 |
IN |
denotes |
of |
T8523 |
2791-2796 |
NN |
denotes |
NADPH |
T8524 |
2797-2799 |
IN |
denotes |
at |
T8525 |
2800-2803 |
CD |
denotes |
340 |
T8526 |
2804-2806 |
NN |
denotes |
nm |
T8527 |
2807-2810 |
CC |
denotes |
and |
T8528 |
2811-2813 |
IN |
denotes |
as |
T8529 |
2814-2823 |
VBN |
denotes |
described |
T8530 |
2824-2826 |
IN |
denotes |
by |
T8531 |
2827-2828 |
-LRB- |
denotes |
[ |
T8532 |
2828-2830 |
CD |
denotes |
66 |
T8533 |
2830-2831 |
-RRB- |
denotes |
] |
T8534 |
2831-2832 |
. |
denotes |
. |
T8535 |
2832-3118 |
sentence |
denotes |
The reaction was started by the addition of purified brain hexokinase I (0.24 μg) (gift from J. Wilson) to 1.0 ml of reaction mixture containing 0.05 M Tris-HCl (pH 8.5), 7.4 mM MgCl2, 6.6 mM ATP, 0.65 mM NADP, 11.1 mM monothioglycerol, and 1 unit of glucose 6-phosphate dehydrogenase. |
T8536 |
2833-2836 |
DT |
denotes |
The |
T8537 |
2837-2845 |
NN |
denotes |
reaction |
T8539 |
2846-2849 |
VBD |
denotes |
was |
T8538 |
2850-2857 |
VBN |
denotes |
started |
T8540 |
2858-2860 |
IN |
denotes |
by |
T8541 |
2861-2864 |
DT |
denotes |
the |
T8542 |
2865-2873 |
NN |
denotes |
addition |
T8543 |
2874-2876 |
IN |
denotes |
of |
T8544 |
2877-2885 |
VBN |
denotes |
purified |
T8546 |
2886-2891 |
NN |
denotes |
brain |
T8545 |
2892-2902 |
NN |
denotes |
hexokinase |
T8547 |
2903-2904 |
CD |
denotes |
I |
T8548 |
2905-2906 |
-LRB- |
denotes |
( |
T8550 |
2906-2910 |
CD |
denotes |
0.24 |
T8549 |
2911-2913 |
NNS |
denotes |
μg |
T8551 |
2913-2914 |
-RRB- |
denotes |
) |
T8552 |
2915-2916 |
-LRB- |
denotes |
( |
T8553 |
2916-2920 |
NN |
denotes |
gift |
T8554 |
2921-2925 |
IN |
denotes |
from |
T8555 |
2926-2928 |
NNP |
denotes |
J. |
T8556 |
2929-2935 |
NNP |
denotes |
Wilson |
T8557 |
2935-2936 |
-RRB- |
denotes |
) |
T8558 |
2937-2939 |
IN |
denotes |
to |
T8559 |
2940-2943 |
CD |
denotes |
1.0 |
T8560 |
2944-2946 |
NN |
denotes |
ml |
T8561 |
2947-2949 |
IN |
denotes |
of |
T8562 |
2950-2958 |
NN |
denotes |
reaction |
T8563 |
2959-2966 |
NN |
denotes |
mixture |
T8564 |
2967-2977 |
VBG |
denotes |
containing |
T8565 |
2978-2982 |
CD |
denotes |
0.05 |
T8566 |
2983-2984 |
NNS |
denotes |
M |
T8568 |
2985-2989 |
NN |
denotes |
Tris |
T8569 |
2989-2990 |
HYPH |
denotes |
- |
T8567 |
2990-2993 |
NN |
denotes |
HCl |
T8570 |
2994-2995 |
-LRB- |
denotes |
( |
T8571 |
2995-2997 |
NN |
denotes |
pH |
T8572 |
2998-3001 |
CD |
denotes |
8.5 |
T8573 |
3001-3002 |
-RRB- |
denotes |
) |
T8574 |
3002-3004 |
, |
denotes |
, |
T8575 |
3004-3007 |
CD |
denotes |
7.4 |
T8576 |
3008-3010 |
NNS |
denotes |
mM |
T8577 |
3011-3016 |
NN |
denotes |
MgCl2 |
T8578 |
3016-3018 |
, |
denotes |
, |
T8579 |
3018-3021 |
CD |
denotes |
6.6 |
T8580 |
3022-3024 |
NNS |
denotes |
mM |
T8581 |
3025-3028 |
NN |
denotes |
ATP |
T8582 |
3028-3030 |
, |
denotes |
, |
T8583 |
3030-3034 |
CD |
denotes |
0.65 |
T8584 |
3035-3037 |
NNS |
denotes |
mM |
T8585 |
3038-3042 |
NN |
denotes |
NADP |
T8586 |
3042-3044 |
, |
denotes |
, |
T8587 |
3044-3048 |
CD |
denotes |
11.1 |
T8588 |
3049-3051 |
NNS |
denotes |
mM |
T8589 |
3052-3068 |
NN |
denotes |
monothioglycerol |
T8590 |
3068-3070 |
, |
denotes |
, |
T8591 |
3070-3073 |
CC |
denotes |
and |
T8592 |
3074-3075 |
CD |
denotes |
1 |
T8593 |
3076-3080 |
NN |
denotes |
unit |
T8594 |
3081-3083 |
IN |
denotes |
of |
T8595 |
3084-3091 |
NN |
denotes |
glucose |
T8597 |
3092-3093 |
CD |
denotes |
6 |
T8598 |
3093-3094 |
HYPH |
denotes |
- |
T8596 |
3094-3103 |
NN |
denotes |
phosphate |
T8599 |
3104-3117 |
NN |
denotes |
dehydrogenase |
T8600 |
3117-3118 |
. |
denotes |
. |
T8601 |
3118-3311 |
sentence |
denotes |
In the case of hexokinase activity measured in the presence of Cox11 and LD, the purified HKI was incubated with the recombinant proteins for 15 min at 4 °C before measurement of the activity. |
T8602 |
3119-3121 |
IN |
denotes |
In |
T8604 |
3122-3125 |
DT |
denotes |
the |
T8605 |
3126-3130 |
NN |
denotes |
case |
T8606 |
3131-3133 |
IN |
denotes |
of |
T8607 |
3134-3144 |
NN |
denotes |
hexokinase |
T8608 |
3145-3153 |
NN |
denotes |
activity |
T8609 |
3154-3162 |
VBN |
denotes |
measured |
T8610 |
3163-3165 |
IN |
denotes |
in |
T8611 |
3166-3169 |
DT |
denotes |
the |
T8612 |
3170-3178 |
NN |
denotes |
presence |
T8613 |
3179-3181 |
IN |
denotes |
of |
T8614 |
3182-3187 |
NN |
denotes |
Cox11 |
T8615 |
3188-3191 |
CC |
denotes |
and |
T8616 |
3192-3194 |
NN |
denotes |
LD |
T8617 |
3194-3196 |
, |
denotes |
, |
T8618 |
3196-3199 |
DT |
denotes |
the |
T8620 |
3200-3208 |
VBN |
denotes |
purified |
T8619 |
3209-3212 |
NN |
denotes |
HKI |
T8621 |
3213-3216 |
VBD |
denotes |
was |
T8603 |
3217-3226 |
VBN |
denotes |
incubated |
T8622 |
3227-3231 |
IN |
denotes |
with |
T8623 |
3232-3235 |
DT |
denotes |
the |
T8625 |
3236-3247 |
JJ |
denotes |
recombinant |
T8624 |
3248-3256 |
NN |
denotes |
proteins |
T8626 |
3257-3260 |
IN |
denotes |
for |
T8627 |
3261-3263 |
CD |
denotes |
15 |
T8628 |
3264-3267 |
NNS |
denotes |
min |
T8629 |
3268-3270 |
IN |
denotes |
at |
T8630 |
3271-3272 |
CD |
denotes |
4 |
T8631 |
3273-3275 |
NN |
denotes |
°C |
T8632 |
3276-3282 |
IN |
denotes |
before |
T8633 |
3283-3294 |
NN |
denotes |
measurement |
T8634 |
3295-3297 |
IN |
denotes |
of |
T8635 |
3298-3301 |
DT |
denotes |
the |
T8636 |
3302-3310 |
NN |
denotes |
activity |
T8637 |
3310-3311 |
. |
denotes |
. |
T8638 |
3311-3408 |
sentence |
denotes |
The data were fitted directly into the Michaelis-Menten equation using SIGMAPLOT (SPSS Science). |
T8639 |
3312-3315 |
DT |
denotes |
The |
T8640 |
3316-3320 |
NNS |
denotes |
data |
T8642 |
3321-3325 |
VBD |
denotes |
were |
T8641 |
3326-3332 |
VBN |
denotes |
fitted |
T8643 |
3333-3341 |
RB |
denotes |
directly |
T8644 |
3342-3346 |
IN |
denotes |
into |
T8645 |
3347-3350 |
DT |
denotes |
the |
T8647 |
3351-3360 |
NNP |
denotes |
Michaelis |
T8649 |
3360-3361 |
HYPH |
denotes |
- |
T8648 |
3361-3367 |
NNP |
denotes |
Menten |
T8646 |
3368-3376 |
NN |
denotes |
equation |
T8650 |
3377-3382 |
VBG |
denotes |
using |
T8651 |
3383-3392 |
NNP |
denotes |
SIGMAPLOT |
T8652 |
3393-3394 |
-LRB- |
denotes |
( |
T8654 |
3394-3398 |
NNP |
denotes |
SPSS |
T8653 |
3399-3406 |
NNP |
denotes |
Science |
T8655 |
3406-3407 |
-RRB- |
denotes |
) |
T8656 |
3407-3408 |
. |
denotes |
. |
T8700 |
3410-3420 |
NNS |
denotes |
Antibodies |
T8701 |
3420-3421 |
. |
denotes |
. |
T8702 |
3421-3601 |
sentence |
denotes |
Rabbit antisera were raised against the recombinant murine Cox11 (residues 40–275) and affinity-purified under non-denaturing conditions (Stereogene) as previously described [13]. |
T8703 |
3422-3428 |
NN |
denotes |
Rabbit |
T8704 |
3429-3437 |
NNS |
denotes |
antisera |
T8706 |
3438-3442 |
VBD |
denotes |
were |
T8705 |
3443-3449 |
VBN |
denotes |
raised |
T8707 |
3450-3457 |
IN |
denotes |
against |
T8708 |
3458-3461 |
DT |
denotes |
the |
T8710 |
3462-3473 |
JJ |
denotes |
recombinant |
T8711 |
3474-3480 |
JJ |
denotes |
murine |
T8709 |
3481-3486 |
NN |
denotes |
Cox11 |
T8712 |
3487-3488 |
-LRB- |
denotes |
( |
T8714 |
3488-3496 |
NNS |
denotes |
residues |
T8713 |
3497-3499 |
CD |
denotes |
40 |
T8715 |
3499-3500 |
SYM |
denotes |
– |
T8716 |
3500-3503 |
CD |
denotes |
275 |
T8717 |
3503-3504 |
-RRB- |
denotes |
) |
T8718 |
3505-3508 |
CC |
denotes |
and |
T8719 |
3509-3517 |
NN |
denotes |
affinity |
T8721 |
3517-3518 |
HYPH |
denotes |
- |
T8720 |
3518-3526 |
VBN |
denotes |
purified |
T8722 |
3527-3532 |
IN |
denotes |
under |
T8723 |
3533-3547 |
NN |
denotes |
non-denaturing |
T8724 |
3548-3558 |
NNS |
denotes |
conditions |
T8725 |
3559-3560 |
-LRB- |
denotes |
( |
T8726 |
3560-3570 |
NNP |
denotes |
Stereogene |
T8727 |
3570-3571 |
-RRB- |
denotes |
) |
T8728 |
3572-3574 |
IN |
denotes |
as |
T8730 |
3575-3585 |
RB |
denotes |
previously |
T8729 |
3586-3595 |
VBN |
denotes |
described |
T8731 |
3596-3597 |
-LRB- |
denotes |
[ |
T8732 |
3597-3599 |
CD |
denotes |
13 |
T8733 |
3599-3600 |
-RRB- |
denotes |
] |
T8734 |
3600-3601 |
. |
denotes |
. |
T8735 |
3601-3663 |
sentence |
denotes |
Cox11 antibodies were used at 200 ng/ml for Western analysis. |
T8736 |
3602-3607 |
NN |
denotes |
Cox11 |
T8737 |
3608-3618 |
NNS |
denotes |
antibodies |
T8739 |
3619-3623 |
VBD |
denotes |
were |
T8738 |
3624-3628 |
VBN |
denotes |
used |
T8740 |
3629-3631 |
IN |
denotes |
at |
T8741 |
3632-3635 |
CD |
denotes |
200 |
T8742 |
3636-3638 |
NNS |
denotes |
ng |
T8743 |
3638-3639 |
SYM |
denotes |
/ |
T8744 |
3639-3641 |
NN |
denotes |
ml |
T8745 |
3642-3645 |
IN |
denotes |
for |
T8746 |
3646-3653 |
NNP |
denotes |
Western |
T8747 |
3654-3662 |
NN |
denotes |
analysis |
T8748 |
3662-3663 |
. |
denotes |
. |
T8749 |
3663-3849 |
sentence |
denotes |
The monoclonal antibody Hsp70 was from Affinity Bioreagents (Golden, Colorado, United States) and antibodies against the KBD (JX2) and ZnF domains of RanBP2 have been described [13,16]. |
T8750 |
3664-3667 |
DT |
denotes |
The |
T8752 |
3668-3678 |
JJ |
denotes |
monoclonal |
T8753 |
3679-3687 |
NN |
denotes |
antibody |
T8751 |
3688-3693 |
NN |
denotes |
Hsp70 |
T8754 |
3694-3697 |
VBD |
denotes |
was |
T8755 |
3698-3702 |
IN |
denotes |
from |
T8756 |
3703-3711 |
NNP |
denotes |
Affinity |
T8757 |
3712-3723 |
NNPS |
denotes |
Bioreagents |
T8758 |
3724-3725 |
-LRB- |
denotes |
( |
T8759 |
3725-3731 |
NNP |
denotes |
Golden |
T8760 |
3731-3733 |
, |
denotes |
, |
T8761 |
3733-3741 |
NNP |
denotes |
Colorado |
T8762 |
3741-3743 |
, |
denotes |
, |
T8763 |
3743-3749 |
NNP |
denotes |
United |
T8764 |
3750-3756 |
NNP |
denotes |
States |
T8765 |
3756-3757 |
-RRB- |
denotes |
) |
T8766 |
3758-3761 |
CC |
denotes |
and |
T8767 |
3762-3772 |
NNS |
denotes |
antibodies |
T8769 |
3773-3780 |
IN |
denotes |
against |
T8770 |
3781-3784 |
DT |
denotes |
the |
T8772 |
3785-3788 |
NN |
denotes |
KBD |
T8773 |
3789-3790 |
-LRB- |
denotes |
( |
T8774 |
3790-3793 |
NN |
denotes |
JX2 |
T8775 |
3793-3794 |
-RRB- |
denotes |
) |
T8776 |
3795-3798 |
CC |
denotes |
and |
T8777 |
3799-3802 |
NN |
denotes |
ZnF |
T8771 |
3803-3810 |
NNS |
denotes |
domains |
T8778 |
3811-3813 |
IN |
denotes |
of |
T8779 |
3814-3820 |
NN |
denotes |
RanBP2 |
T8780 |
3821-3825 |
VBP |
denotes |
have |
T8781 |
3826-3830 |
VBN |
denotes |
been |
T8768 |
3831-3840 |
VBN |
denotes |
described |
T8782 |
3841-3842 |
-LRB- |
denotes |
[ |
T8784 |
3842-3844 |
CD |
denotes |
13 |
T8785 |
3844-3845 |
, |
denotes |
, |
T8783 |
3845-3847 |
CD |
denotes |
16 |
T8786 |
3847-3848 |
-RRB- |
denotes |
] |
T8787 |
3848-3849 |
. |
denotes |
. |
T8788 |
3849-3935 |
sentence |
denotes |
Polyclonal and monoclonal antibodies against Hexokinase I were provided by J. Wilson. |
T8789 |
3850-3860 |
JJ |
denotes |
Polyclonal |
T8791 |
3861-3864 |
CC |
denotes |
and |
T8792 |
3865-3875 |
JJ |
denotes |
monoclonal |
T8790 |
3876-3886 |
NNS |
denotes |
antibodies |
T8794 |
3887-3894 |
IN |
denotes |
against |
T8795 |
3895-3905 |
NN |
denotes |
Hexokinase |
T8796 |
3906-3907 |
PRP |
denotes |
I |
T8797 |
3908-3912 |
VBD |
denotes |
were |
T8793 |
3913-3921 |
VBN |
denotes |
provided |
T8798 |
3922-3924 |
IN |
denotes |
by |
T8799 |
3925-3927 |
NNP |
denotes |
J. |
T8800 |
3928-3934 |
NNP |
denotes |
Wilson |
T8801 |
3934-3935 |
. |
denotes |
. |
T8802 |
3935-4014 |
sentence |
denotes |
The mAb414 was purchased from Abcam (Cambridge, Massachusetts, United States). |
T8803 |
3936-3939 |
DT |
denotes |
The |
T8804 |
3940-3946 |
NN |
denotes |
mAb414 |
T8806 |
3947-3950 |
VBD |
denotes |
was |
T8805 |
3951-3960 |
VBN |
denotes |
purchased |
T8807 |
3961-3965 |
IN |
denotes |
from |
T8808 |
3966-3971 |
NNP |
denotes |
Abcam |
T8809 |
3972-3973 |
-LRB- |
denotes |
( |
T8810 |
3973-3982 |
NNP |
denotes |
Cambridge |
T8811 |
3982-3984 |
, |
denotes |
, |
T8812 |
3984-3997 |
NNP |
denotes |
Massachusetts |
T8813 |
3997-3999 |
, |
denotes |
, |
T8814 |
3999-4005 |
NNP |
denotes |
United |
T8815 |
4006-4012 |
NNP |
denotes |
States |
T8816 |
4012-4013 |
-RRB- |
denotes |
) |
T8817 |
4013-4014 |
. |
denotes |
. |
T8883 |
4016-4035 |
NN |
denotes |
Immunocytochemistry |
T8884 |
4036-4039 |
CC |
denotes |
and |
T8885 |
4040-4050 |
NN |
denotes |
microscopy |
T8886 |
4050-4051 |
. |
denotes |
. |
T8887 |
4051-4182 |
sentence |
denotes |
Retina dissections, radial cryosections (~6 μm), and immunohistochemistry procedures were carried out as described elsewhere [19]. |
T8888 |
4052-4058 |
NN |
denotes |
Retina |
T8889 |
4059-4070 |
NNS |
denotes |
dissections |
T8891 |
4070-4072 |
, |
denotes |
, |
T8892 |
4072-4078 |
JJ |
denotes |
radial |
T8893 |
4079-4091 |
NNS |
denotes |
cryosections |
T8894 |
4092-4093 |
-LRB- |
denotes |
( |
T8896 |
4093-4094 |
SYM |
denotes |
~ |
T8897 |
4094-4095 |
CD |
denotes |
6 |
T8895 |
4096-4098 |
NNS |
denotes |
μm |
T8898 |
4098-4099 |
-RRB- |
denotes |
) |
T8899 |
4099-4101 |
, |
denotes |
, |
T8900 |
4101-4104 |
CC |
denotes |
and |
T8901 |
4105-4125 |
NN |
denotes |
immunohistochemistry |
T8902 |
4126-4136 |
NNS |
denotes |
procedures |
T8903 |
4137-4141 |
VBD |
denotes |
were |
T8890 |
4142-4149 |
VBN |
denotes |
carried |
T8904 |
4150-4153 |
RP |
denotes |
out |
T8905 |
4154-4156 |
IN |
denotes |
as |
T8906 |
4157-4166 |
VBN |
denotes |
described |
T8907 |
4167-4176 |
RB |
denotes |
elsewhere |
T8908 |
4177-4178 |
-LRB- |
denotes |
[ |
T8909 |
4178-4180 |
CD |
denotes |
19 |
T8910 |
4180-4181 |
-RRB- |
denotes |
] |
T8911 |
4181-4182 |
. |
denotes |
. |
T8912 |
4182-4327 |
sentence |
denotes |
Brains from 3- to 5-mo-old C57Bl/6 mice were fixed overnight in 2% paraformaldehyde, infused with 30% sucrose, and processed for cryosectioning. |
T8913 |
4183-4189 |
NNS |
denotes |
Brains |
T8915 |
4190-4194 |
IN |
denotes |
from |
T8916 |
4195-4196 |
CD |
denotes |
3 |
T8918 |
4196-4197 |
HYPH |
denotes |
- |
T8919 |
4198-4200 |
IN |
denotes |
to |
T8917 |
4201-4202 |
CD |
denotes |
5 |
T8921 |
4202-4203 |
HYPH |
denotes |
- |
T8920 |
4203-4205 |
NN |
denotes |
mo |
T8923 |
4205-4206 |
HYPH |
denotes |
- |
T8922 |
4206-4209 |
JJ |
denotes |
old |
T8925 |
4210-4215 |
NN |
denotes |
C57Bl |
T8926 |
4215-4216 |
HYPH |
denotes |
/ |
T8927 |
4216-4217 |
CD |
denotes |
6 |
T8924 |
4218-4222 |
NNS |
denotes |
mice |
T8928 |
4223-4227 |
VBD |
denotes |
were |
T8914 |
4228-4233 |
VBN |
denotes |
fixed |
T8929 |
4234-4243 |
RB |
denotes |
overnight |
T8930 |
4244-4246 |
IN |
denotes |
in |
T8931 |
4247-4248 |
CD |
denotes |
2 |
T8932 |
4248-4249 |
NN |
denotes |
% |
T8933 |
4250-4266 |
NN |
denotes |
paraformaldehyde |
T8934 |
4266-4268 |
, |
denotes |
, |
T8935 |
4268-4275 |
VBN |
denotes |
infused |
T8936 |
4276-4280 |
IN |
denotes |
with |
T8937 |
4281-4283 |
CD |
denotes |
30 |
T8938 |
4283-4284 |
NN |
denotes |
% |
T8939 |
4285-4292 |
NN |
denotes |
sucrose |
T8940 |
4292-4294 |
, |
denotes |
, |
T8941 |
4294-4297 |
CC |
denotes |
and |
T8942 |
4298-4307 |
VBN |
denotes |
processed |
T8943 |
4308-4311 |
IN |
denotes |
for |
T8944 |
4312-4326 |
VBG |
denotes |
cryosectioning |
T8945 |
4326-4327 |
. |
denotes |
. |
T8946 |
4327-4405 |
sentence |
denotes |
Primary brain neurons and glial cells were prepared from the cerebral cortex. |
T8947 |
4328-4335 |
JJ |
denotes |
Primary |
T8949 |
4336-4341 |
NN |
denotes |
brain |
T8948 |
4342-4349 |
NNS |
denotes |
neurons |
T8951 |
4350-4353 |
CC |
denotes |
and |
T8952 |
4354-4359 |
JJ |
denotes |
glial |
T8953 |
4360-4365 |
NNS |
denotes |
cells |
T8954 |
4366-4370 |
VBD |
denotes |
were |
T8950 |
4371-4379 |
VBN |
denotes |
prepared |
T8955 |
4380-4384 |
IN |
denotes |
from |
T8956 |
4385-4388 |
DT |
denotes |
the |
T8958 |
4389-4397 |
JJ |
denotes |
cerebral |
T8957 |
4398-4404 |
NN |
denotes |
cortex |
T8959 |
4404-4405 |
. |
denotes |
. |
T8960 |
4405-4477 |
sentence |
denotes |
This was macerated in the Hank's Balanced Salt solution and triturated. |
T8961 |
4406-4410 |
DT |
denotes |
This |
T8963 |
4411-4414 |
VBD |
denotes |
was |
T8962 |
4415-4424 |
VBN |
denotes |
macerated |
T8964 |
4425-4427 |
IN |
denotes |
in |
T8965 |
4428-4431 |
DT |
denotes |
the |
T8967 |
4432-4436 |
NNP |
denotes |
Hank |
T8969 |
4436-4438 |
POS |
denotes |
's |
T8970 |
4439-4447 |
NNP |
denotes |
Balanced |
T8968 |
4448-4452 |
NNP |
denotes |
Salt |
T8966 |
4453-4461 |
NN |
denotes |
solution |
T8971 |
4462-4465 |
CC |
denotes |
and |
T8972 |
4466-4476 |
VBN |
denotes |
triturated |
T8973 |
4476-4477 |
. |
denotes |
. |
T8974 |
4477-4714 |
sentence |
denotes |
Primary cells were cultured in DMEM (GIBCO, San Diego, California, United States) and collagen-coated 35-mm glass bottom culture dishes (MatTek Corporation, Ashland, Maine) at 5% CO2/37 °C for ~2 d and processed for immunocytochemistry. |
T8975 |
4478-4485 |
JJ |
denotes |
Primary |
T8976 |
4486-4491 |
NNS |
denotes |
cells |
T8978 |
4492-4496 |
VBD |
denotes |
were |
T8977 |
4497-4505 |
VBN |
denotes |
cultured |
T8979 |
4506-4508 |
IN |
denotes |
in |
T8980 |
4509-4513 |
NN |
denotes |
DMEM |
T8981 |
4514-4515 |
-LRB- |
denotes |
( |
T8982 |
4515-4520 |
NNP |
denotes |
GIBCO |
T8983 |
4520-4522 |
, |
denotes |
, |
T8984 |
4522-4525 |
NNP |
denotes |
San |
T8985 |
4526-4531 |
NNP |
denotes |
Diego |
T8986 |
4531-4533 |
, |
denotes |
, |
T8987 |
4533-4543 |
NNP |
denotes |
California |
T8988 |
4543-4545 |
, |
denotes |
, |
T8989 |
4545-4551 |
NNP |
denotes |
United |
T8990 |
4552-4558 |
NNP |
denotes |
States |
T8991 |
4558-4559 |
-RRB- |
denotes |
) |
T8992 |
4560-4563 |
CC |
denotes |
and |
T8993 |
4564-4572 |
NN |
denotes |
collagen |
T8995 |
4572-4573 |
HYPH |
denotes |
- |
T8994 |
4573-4579 |
VBN |
denotes |
coated |
T8997 |
4580-4582 |
CD |
denotes |
35 |
T8999 |
4582-4583 |
HYPH |
denotes |
- |
T8998 |
4583-4585 |
NN |
denotes |
mm |
T9000 |
4586-4591 |
NN |
denotes |
glass |
T9001 |
4592-4598 |
NN |
denotes |
bottom |
T9002 |
4599-4606 |
NN |
denotes |
culture |
T8996 |
4607-4613 |
NNS |
denotes |
dishes |
T9003 |
4614-4615 |
-LRB- |
denotes |
( |
T9005 |
4615-4621 |
NNP |
denotes |
MatTek |
T9004 |
4622-4633 |
NNP |
denotes |
Corporation |
T9006 |
4633-4635 |
, |
denotes |
, |
T9007 |
4635-4642 |
NNP |
denotes |
Ashland |
T9008 |
4642-4644 |
, |
denotes |
, |
T9009 |
4644-4649 |
NNP |
denotes |
Maine |
T9010 |
4649-4650 |
-RRB- |
denotes |
) |
T9011 |
4651-4653 |
IN |
denotes |
at |
T9012 |
4654-4655 |
CD |
denotes |
5 |
T9013 |
4655-4656 |
NN |
denotes |
% |
T9014 |
4657-4660 |
NN |
denotes |
CO2 |
T9015 |
4660-4661 |
HYPH |
denotes |
/ |
T9016 |
4661-4663 |
CD |
denotes |
37 |
T9017 |
4664-4666 |
NN |
denotes |
°C |
T9018 |
4667-4670 |
IN |
denotes |
for |
T9019 |
4671-4672 |
SYM |
denotes |
~ |
T9020 |
4672-4673 |
CD |
denotes |
2 |
T9021 |
4674-4675 |
NNS |
denotes |
d |
T9022 |
4676-4679 |
CC |
denotes |
and |
T9023 |
4680-4689 |
VBN |
denotes |
processed |
T9024 |
4690-4693 |
IN |
denotes |
for |
T9025 |
4694-4713 |
NN |
denotes |
immunocytochemistry |
T9026 |
4713-4714 |
. |
denotes |
. |
T9027 |
4714-4776 |
sentence |
denotes |
Primary antibodies were used at concentrations ~2.5–10 μg/ml. |
T9028 |
4715-4722 |
JJ |
denotes |
Primary |
T9029 |
4723-4733 |
NNS |
denotes |
antibodies |
T9031 |
4734-4738 |
VBD |
denotes |
were |
T9030 |
4739-4743 |
VBN |
denotes |
used |
T9032 |
4744-4746 |
IN |
denotes |
at |
T9033 |
4747-4761 |
NNS |
denotes |
concentrations |
T9034 |
4762-4763 |
SYM |
denotes |
~ |
T9036 |
4763-4766 |
CD |
denotes |
2.5 |
T9037 |
4766-4767 |
SYM |
denotes |
– |
T9035 |
4767-4769 |
CD |
denotes |
10 |
T9038 |
4770-4772 |
NN |
denotes |
μg |
T9039 |
4772-4773 |
SYM |
denotes |
/ |
T9040 |
4773-4775 |
NN |
denotes |
ml |
T9041 |
4775-4776 |
. |
denotes |
. |
T9042 |
4776-4905 |
sentence |
denotes |
Alexa 488- and Alexa 594-conjugated secondary antibodies (2.5 μg/ml) (Molecular Probes) were used for visualization of proteins. |
T9043 |
4777-4782 |
NNP |
denotes |
Alexa |
T9045 |
4783-4786 |
CD |
denotes |
488 |
T9046 |
4786-4787 |
HYPH |
denotes |
- |
T9047 |
4788-4791 |
CC |
denotes |
and |
T9048 |
4792-4797 |
NNP |
denotes |
Alexa |
T9049 |
4798-4801 |
JJ |
denotes |
594 |
T9050 |
4801-4802 |
HYPH |
denotes |
- |
T9044 |
4802-4812 |
JJ |
denotes |
conjugated |
T9052 |
4813-4822 |
JJ |
denotes |
secondary |
T9051 |
4823-4833 |
NNS |
denotes |
antibodies |
T9054 |
4834-4835 |
-LRB- |
denotes |
( |
T9056 |
4835-4838 |
CD |
denotes |
2.5 |
T9055 |
4839-4841 |
NNS |
denotes |
μg |
T9057 |
4841-4842 |
SYM |
denotes |
/ |
T9058 |
4842-4844 |
NN |
denotes |
ml |
T9059 |
4844-4845 |
-RRB- |
denotes |
) |
T9060 |
4846-4847 |
-LRB- |
denotes |
( |
T9062 |
4847-4856 |
NNP |
denotes |
Molecular |
T9061 |
4857-4863 |
NNP |
denotes |
Probes |
T9063 |
4863-4864 |
-RRB- |
denotes |
) |
T9064 |
4865-4869 |
VBD |
denotes |
were |
T9053 |
4870-4874 |
VBN |
denotes |
used |
T9065 |
4875-4878 |
IN |
denotes |
for |
T9066 |
4879-4892 |
NN |
denotes |
visualization |
T9067 |
4893-4895 |
IN |
denotes |
of |
T9068 |
4896-4904 |
NN |
denotes |
proteins |
T9069 |
4904-4905 |
. |
denotes |
. |
T9070 |
4905-5000 |
sentence |
denotes |
Crossover of fluorescent probes, background, and autofluorescence were found to be negligible. |
T9071 |
4906-4915 |
NN |
denotes |
Crossover |
T9073 |
4916-4918 |
IN |
denotes |
of |
T9074 |
4919-4930 |
JJ |
denotes |
fluorescent |
T9075 |
4931-4937 |
NNS |
denotes |
probes |
T9076 |
4937-4939 |
, |
denotes |
, |
T9077 |
4939-4949 |
NN |
denotes |
background |
T9078 |
4949-4951 |
, |
denotes |
, |
T9079 |
4951-4954 |
CC |
denotes |
and |
T9080 |
4955-4971 |
NN |
denotes |
autofluorescence |
T9081 |
4972-4976 |
VBD |
denotes |
were |
T9072 |
4977-4982 |
VBN |
denotes |
found |
T9082 |
4983-4985 |
TO |
denotes |
to |
T9083 |
4986-4988 |
VB |
denotes |
be |
T9084 |
4989-4999 |
JJ |
denotes |
negligible |
T9085 |
4999-5000 |
. |
denotes |
. |
T9086 |
5000-5238 |
sentence |
denotes |
Visualization of specimens and localization of proteins were carried out by wide-field epifluorescence microscopy on Nikon (Tokyo, Japan) E600 upright and TE2000U inverted research microscopes equipped with similar Apochromat objectives. |
T9087 |
5001-5014 |
NN |
denotes |
Visualization |
T9089 |
5015-5017 |
IN |
denotes |
of |
T9090 |
5018-5027 |
NNS |
denotes |
specimens |
T9091 |
5028-5031 |
CC |
denotes |
and |
T9092 |
5032-5044 |
NN |
denotes |
localization |
T9093 |
5045-5047 |
IN |
denotes |
of |
T9094 |
5048-5056 |
NN |
denotes |
proteins |
T9095 |
5057-5061 |
VBD |
denotes |
were |
T9088 |
5062-5069 |
VBN |
denotes |
carried |
T9096 |
5070-5073 |
RP |
denotes |
out |
T9097 |
5074-5076 |
IN |
denotes |
by |
T9098 |
5077-5081 |
JJ |
denotes |
wide |
T9100 |
5081-5082 |
HYPH |
denotes |
- |
T9099 |
5082-5087 |
NN |
denotes |
field |
T9102 |
5088-5103 |
NN |
denotes |
epifluorescence |
T9101 |
5104-5114 |
NN |
denotes |
microscopy |
T9103 |
5115-5117 |
IN |
denotes |
on |
T9104 |
5118-5123 |
NNP |
denotes |
Nikon |
T9106 |
5124-5125 |
-LRB- |
denotes |
( |
T9107 |
5125-5130 |
NNP |
denotes |
Tokyo |
T9108 |
5130-5132 |
, |
denotes |
, |
T9109 |
5132-5137 |
NNP |
denotes |
Japan |
T9110 |
5137-5138 |
-RRB- |
denotes |
) |
T9111 |
5139-5143 |
NN |
denotes |
E600 |
T9105 |
5144-5151 |
NN |
denotes |
upright |
T9112 |
5152-5155 |
CC |
denotes |
and |
T9113 |
5156-5163 |
NN |
denotes |
TE2000U |
T9115 |
5164-5172 |
VBN |
denotes |
inverted |
T9116 |
5173-5181 |
NN |
denotes |
research |
T9114 |
5182-5193 |
NNS |
denotes |
microscopes |
T9117 |
5194-5202 |
VBN |
denotes |
equipped |
T9118 |
5203-5207 |
IN |
denotes |
with |
T9119 |
5208-5215 |
JJ |
denotes |
similar |
T9121 |
5216-5226 |
NN |
denotes |
Apochromat |
T9120 |
5227-5237 |
NNS |
denotes |
objectives |
T9122 |
5237-5238 |
. |
denotes |
. |
T9123 |
5238-5445 |
sentence |
denotes |
Images with the Nikon E600 were acquired with a SPOT-RT digital camera coupled to the microscope and driven by SPOT Imaging v4.0 software (Diagnostic Instruments, Sterling Heights, Michigan, United States). |
T9124 |
5239-5245 |
NNS |
denotes |
Images |
T9126 |
5246-5250 |
IN |
denotes |
with |
T9127 |
5251-5254 |
DT |
denotes |
the |
T9129 |
5255-5260 |
NNP |
denotes |
Nikon |
T9128 |
5261-5265 |
NN |
denotes |
E600 |
T9130 |
5266-5270 |
VBD |
denotes |
were |
T9125 |
5271-5279 |
VBN |
denotes |
acquired |
T9131 |
5280-5284 |
IN |
denotes |
with |
T9132 |
5285-5286 |
DT |
denotes |
a |
T9134 |
5287-5291 |
NN |
denotes |
SPOT |
T9136 |
5291-5292 |
HYPH |
denotes |
- |
T9135 |
5292-5294 |
NN |
denotes |
RT |
T9137 |
5295-5302 |
JJ |
denotes |
digital |
T9133 |
5303-5309 |
NN |
denotes |
camera |
T9138 |
5310-5317 |
VBN |
denotes |
coupled |
T9139 |
5318-5320 |
IN |
denotes |
to |
T9140 |
5321-5324 |
DT |
denotes |
the |
T9141 |
5325-5335 |
NN |
denotes |
microscope |
T9142 |
5336-5339 |
CC |
denotes |
and |
T9143 |
5340-5346 |
VBN |
denotes |
driven |
T9144 |
5347-5349 |
IN |
denotes |
by |
T9145 |
5350-5354 |
NN |
denotes |
SPOT |
T9147 |
5355-5362 |
NN |
denotes |
Imaging |
T9146 |
5363-5367 |
NN |
denotes |
v4.0 |
T9148 |
5368-5376 |
NN |
denotes |
software |
T9149 |
5377-5378 |
-LRB- |
denotes |
( |
T9151 |
5378-5388 |
NNP |
denotes |
Diagnostic |
T9150 |
5389-5400 |
NNPS |
denotes |
Instruments |
T9152 |
5400-5402 |
, |
denotes |
, |
T9153 |
5402-5410 |
NNP |
denotes |
Sterling |
T9154 |
5411-5418 |
NNP |
denotes |
Heights |
T9155 |
5418-5420 |
, |
denotes |
, |
T9156 |
5420-5428 |
NNP |
denotes |
Michigan |
T9157 |
5428-5430 |
, |
denotes |
, |
T9158 |
5430-5436 |
NNP |
denotes |
United |
T9159 |
5437-5443 |
NNP |
denotes |
States |
T9160 |
5443-5444 |
-RRB- |
denotes |
) |
T9161 |
5444-5445 |
. |
denotes |
. |
T9162 |
5445-5557 |
sentence |
denotes |
All images were captured at nonsaturating integration levels, 12-bit mono black/white, and then pseudo-colored. |
T9163 |
5446-5449 |
DT |
denotes |
All |
T9164 |
5450-5456 |
NNS |
denotes |
images |
T9166 |
5457-5461 |
VBD |
denotes |
were |
T9165 |
5462-5470 |
VBN |
denotes |
captured |
T9167 |
5471-5473 |
IN |
denotes |
at |
T9168 |
5474-5487 |
NN |
denotes |
nonsaturating |
T9170 |
5488-5499 |
NN |
denotes |
integration |
T9169 |
5500-5506 |
NNS |
denotes |
levels |
T9171 |
5506-5508 |
, |
denotes |
, |
T9172 |
5508-5510 |
CD |
denotes |
12 |
T9174 |
5510-5511 |
HYPH |
denotes |
- |
T9173 |
5511-5514 |
JJ |
denotes |
bit |
T9176 |
5515-5519 |
NN |
denotes |
mono |
T9177 |
5520-5525 |
JJ |
denotes |
black |
T9178 |
5525-5526 |
HYPH |
denotes |
/ |
T9175 |
5526-5531 |
JJ |
denotes |
white |
T9179 |
5531-5533 |
, |
denotes |
, |
T9180 |
5533-5536 |
CC |
denotes |
and |
T9181 |
5537-5541 |
RB |
denotes |
then |
T9182 |
5542-5556 |
VBN |
denotes |
pseudo-colored |
T9183 |
5556-5557 |
. |
denotes |
. |
T9184 |
5557-5953 |
sentence |
denotes |
Protein colocalization analysis was carried out on the Nikon TE2000U microscope equipped with appropriate excitation and emission filter wheels, cube filters, 100 W mercury light source, Nomarski/DIC, and Plan Apochromat optics (100×, 60×, and 40× oil objectives with NA of 1.4, 40 ×LWD, and 20 ×LWD objectives and encoded motorized Z-Stage (Prior Scientific, Rockland, Maryland, United States). |
T9185 |
5558-5565 |
NN |
denotes |
Protein |
T9186 |
5566-5580 |
NN |
denotes |
colocalization |
T9187 |
5581-5589 |
NN |
denotes |
analysis |
T9189 |
5590-5593 |
VBD |
denotes |
was |
T9188 |
5594-5601 |
VBN |
denotes |
carried |
T9190 |
5602-5605 |
RP |
denotes |
out |
T9191 |
5606-5608 |
IN |
denotes |
on |
T9192 |
5609-5612 |
DT |
denotes |
the |
T9194 |
5613-5618 |
NNP |
denotes |
Nikon |
T9195 |
5619-5626 |
NN |
denotes |
TE2000U |
T9193 |
5627-5637 |
NN |
denotes |
microscope |
T9196 |
5638-5646 |
VBN |
denotes |
equipped |
T9197 |
5647-5651 |
IN |
denotes |
with |
T9198 |
5652-5663 |
JJ |
denotes |
appropriate |
T9200 |
5664-5674 |
NN |
denotes |
excitation |
T9201 |
5675-5678 |
CC |
denotes |
and |
T9202 |
5679-5687 |
NN |
denotes |
emission |
T9203 |
5688-5694 |
NN |
denotes |
filter |
T9199 |
5695-5701 |
NNS |
denotes |
wheels |
T9204 |
5701-5703 |
, |
denotes |
, |
T9205 |
5703-5707 |
NN |
denotes |
cube |
T9206 |
5708-5715 |
NNS |
denotes |
filters |
T9207 |
5715-5717 |
, |
denotes |
, |
T9208 |
5717-5720 |
CD |
denotes |
100 |
T9209 |
5721-5722 |
NNP |
denotes |
W |
T9211 |
5723-5730 |
NN |
denotes |
mercury |
T9212 |
5731-5736 |
NN |
denotes |
light |
T9210 |
5737-5743 |
NN |
denotes |
source |
T9213 |
5743-5745 |
, |
denotes |
, |
T9214 |
5745-5753 |
NNP |
denotes |
Nomarski |
T9216 |
5753-5754 |
HYPH |
denotes |
/ |
T9215 |
5754-5757 |
NNP |
denotes |
DIC |
T9218 |
5757-5759 |
, |
denotes |
, |
T9219 |
5759-5762 |
CC |
denotes |
and |
T9220 |
5763-5767 |
NNP |
denotes |
Plan |
T9221 |
5768-5778 |
NNP |
denotes |
Apochromat |
T9217 |
5779-5785 |
NNS |
denotes |
optics |
T9222 |
5786-5787 |
-LRB- |
denotes |
( |
T9223 |
5787-5790 |
CD |
denotes |
100 |
T9225 |
5790-5791 |
SYM |
denotes |
× |
T9226 |
5791-5793 |
, |
denotes |
, |
T9227 |
5793-5795 |
CD |
denotes |
60 |
T9228 |
5795-5796 |
SYM |
denotes |
× |
T9229 |
5796-5798 |
, |
denotes |
, |
T9230 |
5798-5801 |
CC |
denotes |
and |
T9231 |
5802-5804 |
CD |
denotes |
40 |
T9232 |
5804-5805 |
SYM |
denotes |
× |
T9233 |
5806-5809 |
NN |
denotes |
oil |
T9224 |
5810-5820 |
NNS |
denotes |
objectives |
T9234 |
5821-5825 |
IN |
denotes |
with |
T9235 |
5826-5828 |
NN |
denotes |
NA |
T9236 |
5829-5831 |
IN |
denotes |
of |
T9237 |
5832-5835 |
CD |
denotes |
1.4 |
T9238 |
5835-5837 |
, |
denotes |
, |
T9239 |
5837-5839 |
CD |
denotes |
40 |
T9241 |
5840-5841 |
SYM |
denotes |
× |
T9240 |
5841-5844 |
NN |
denotes |
LWD |
T9243 |
5844-5846 |
, |
denotes |
, |
T9244 |
5846-5849 |
CC |
denotes |
and |
T9245 |
5850-5852 |
CD |
denotes |
20 |
T9247 |
5853-5854 |
SYM |
denotes |
× |
T9246 |
5854-5857 |
NN |
denotes |
LWD |
T9242 |
5858-5868 |
NNS |
denotes |
objectives |
T9248 |
5869-5872 |
CC |
denotes |
and |
T9249 |
5873-5880 |
VBN |
denotes |
encoded |
T9251 |
5881-5890 |
VBN |
denotes |
motorized |
T9252 |
5891-5892 |
NNP |
denotes |
Z |
T9253 |
5892-5893 |
HYPH |
denotes |
- |
T9250 |
5893-5898 |
NNP |
denotes |
Stage |
T9254 |
5899-5900 |
-LRB- |
denotes |
( |
T9256 |
5900-5905 |
NNP |
denotes |
Prior |
T9255 |
5906-5916 |
NNP |
denotes |
Scientific |
T9257 |
5916-5918 |
, |
denotes |
, |
T9258 |
5918-5926 |
NNP |
denotes |
Rockland |
T9259 |
5926-5928 |
, |
denotes |
, |
T9260 |
5928-5936 |
NNP |
denotes |
Maryland |
T9261 |
5936-5938 |
, |
denotes |
, |
T9262 |
5938-5944 |
NNP |
denotes |
United |
T9263 |
5945-5951 |
NNP |
denotes |
States |
T9264 |
5951-5952 |
-RRB- |
denotes |
) |
T9265 |
5952-5953 |
. |
denotes |
. |
T9266 |
5953-6105 |
sentence |
denotes |
Images with the inverted Nikon TE2000U microscope were obtained using a CCD camera (CoolSNAP HQ; Roper Scientific, Trenton, New Jersey, United States). |
T9267 |
5954-5960 |
NNS |
denotes |
Images |
T9269 |
5961-5965 |
IN |
denotes |
with |
T9270 |
5966-5969 |
DT |
denotes |
the |
T9272 |
5970-5978 |
VBN |
denotes |
inverted |
T9273 |
5979-5984 |
NNP |
denotes |
Nikon |
T9274 |
5985-5992 |
NN |
denotes |
TE2000U |
T9271 |
5993-6003 |
NN |
denotes |
microscope |
T9275 |
6004-6008 |
VBD |
denotes |
were |
T9268 |
6009-6017 |
VBN |
denotes |
obtained |
T9276 |
6018-6023 |
VBG |
denotes |
using |
T9277 |
6024-6025 |
DT |
denotes |
a |
T9279 |
6026-6029 |
NN |
denotes |
CCD |
T9278 |
6030-6036 |
NN |
denotes |
camera |
T9280 |
6037-6038 |
-LRB- |
denotes |
( |
T9281 |
6038-6046 |
NNP |
denotes |
CoolSNAP |
T9282 |
6047-6049 |
NNP |
denotes |
HQ |
T9283 |
6049-6050 |
: |
denotes |
; |
T9285 |
6051-6056 |
NNP |
denotes |
Roper |
T9284 |
6057-6067 |
NNP |
denotes |
Scientific |
T9286 |
6067-6069 |
, |
denotes |
, |
T9287 |
6069-6076 |
NNP |
denotes |
Trenton |
T9288 |
6076-6078 |
, |
denotes |
, |
T9289 |
6078-6081 |
NNP |
denotes |
New |
T9290 |
6082-6088 |
NNP |
denotes |
Jersey |
T9291 |
6088-6090 |
, |
denotes |
, |
T9292 |
6090-6096 |
NNP |
denotes |
United |
T9293 |
6097-6103 |
NNP |
denotes |
States |
T9294 |
6103-6104 |
-RRB- |
denotes |
) |
T9295 |
6104-6105 |
. |
denotes |
. |
T9296 |
6105-6261 |
sentence |
denotes |
Images acquired under identical acquisition parameters were analyzed using Metamorph Software v6.2 (Universal Imaging, Glendale, Wisconsin, United States). |
T9297 |
6106-6112 |
NNS |
denotes |
Images |
T9299 |
6113-6121 |
VBN |
denotes |
acquired |
T9300 |
6122-6127 |
IN |
denotes |
under |
T9301 |
6128-6137 |
JJ |
denotes |
identical |
T9303 |
6138-6149 |
NN |
denotes |
acquisition |
T9302 |
6150-6160 |
NNS |
denotes |
parameters |
T9304 |
6161-6165 |
VBD |
denotes |
were |
T9298 |
6166-6174 |
VBN |
denotes |
analyzed |
T9305 |
6175-6180 |
VBG |
denotes |
using |
T9306 |
6181-6190 |
NNP |
denotes |
Metamorph |
T9308 |
6191-6199 |
NNP |
denotes |
Software |
T9307 |
6200-6204 |
NN |
denotes |
v6.2 |
T9309 |
6205-6206 |
-LRB- |
denotes |
( |
T9311 |
6206-6215 |
NNP |
denotes |
Universal |
T9310 |
6216-6223 |
NNP |
denotes |
Imaging |
T9312 |
6223-6225 |
, |
denotes |
, |
T9313 |
6225-6233 |
NNP |
denotes |
Glendale |
T9314 |
6233-6235 |
, |
denotes |
, |
T9315 |
6235-6244 |
NNP |
denotes |
Wisconsin |
T9316 |
6244-6246 |
, |
denotes |
, |
T9317 |
6246-6252 |
NNP |
denotes |
United |
T9318 |
6253-6259 |
NNP |
denotes |
States |
T9319 |
6259-6260 |
-RRB- |
denotes |
) |
T9320 |
6260-6261 |
. |
denotes |
. |
T9321 |
6261-6450 |
sentence |
denotes |
Whenever applicable, serial optical Z-stacks (20–30 focal planes at 100-nm intervals) were captured and computationally processed by 3-D blind deconvolution methods with the same software. |
T9322 |
6262-6270 |
RB |
denotes |
Whenever |
T9323 |
6271-6281 |
JJ |
denotes |
applicable |
T9325 |
6281-6283 |
, |
denotes |
, |
T9326 |
6283-6289 |
JJ |
denotes |
serial |
T9328 |
6290-6297 |
JJ |
denotes |
optical |
T9329 |
6298-6299 |
NN |
denotes |
Z |
T9330 |
6299-6300 |
HYPH |
denotes |
- |
T9327 |
6300-6306 |
NNS |
denotes |
stacks |
T9331 |
6307-6308 |
-LRB- |
denotes |
( |
T9332 |
6308-6310 |
CD |
denotes |
20 |
T9334 |
6310-6311 |
SYM |
denotes |
– |
T9333 |
6311-6313 |
CD |
denotes |
30 |
T9336 |
6314-6319 |
JJ |
denotes |
focal |
T9335 |
6320-6326 |
NNS |
denotes |
planes |
T9337 |
6327-6329 |
IN |
denotes |
at |
T9338 |
6330-6333 |
CD |
denotes |
100 |
T9340 |
6333-6334 |
HYPH |
denotes |
- |
T9339 |
6334-6336 |
NN |
denotes |
nm |
T9341 |
6337-6346 |
NNS |
denotes |
intervals |
T9342 |
6346-6347 |
-RRB- |
denotes |
) |
T9343 |
6348-6352 |
VBD |
denotes |
were |
T9324 |
6353-6361 |
VBN |
denotes |
captured |
T9344 |
6362-6365 |
CC |
denotes |
and |
T9345 |
6366-6381 |
RB |
denotes |
computationally |
T9346 |
6382-6391 |
VBN |
denotes |
processed |
T9347 |
6392-6394 |
IN |
denotes |
by |
T9348 |
6395-6396 |
CD |
denotes |
3 |
T9350 |
6396-6397 |
HYPH |
denotes |
- |
T9349 |
6397-6398 |
NN |
denotes |
D |
T9352 |
6399-6404 |
JJ |
denotes |
blind |
T9353 |
6405-6418 |
NN |
denotes |
deconvolution |
T9351 |
6419-6426 |
NNS |
denotes |
methods |
T9354 |
6427-6431 |
IN |
denotes |
with |
T9355 |
6432-6435 |
DT |
denotes |
the |
T9357 |
6436-6440 |
JJ |
denotes |
same |
T9356 |
6441-6449 |
NN |
denotes |
software |
T9358 |
6449-6450 |
. |
denotes |
. |
T9491 |
6452-6462 |
NN |
denotes |
Generation |
T9492 |
6463-6465 |
IN |
denotes |
of |
T9493 |
6466-6467 |
DT |
denotes |
a |
T9495 |
6468-6473 |
NN |
denotes |
mouse |
T9494 |
6474-6478 |
NN |
denotes |
line |
T9496 |
6479-6483 |
IN |
denotes |
with |
T9497 |
6484-6485 |
DT |
denotes |
a |
T9499 |
6486-6495 |
VBN |
denotes |
disrupted |
T9500 |
6496-6502 |
NN |
denotes |
RanBP2 |
T9498 |
6503-6508 |
NN |
denotes |
locus |
T9501 |
6509-6512 |
CC |
denotes |
and |
T9502 |
6513-6525 |
JJ |
denotes |
histological |
T9503 |
6526-6534 |
NN |
denotes |
analysis |
T9504 |
6535-6537 |
IN |
denotes |
of |
T9505 |
6538-6545 |
VBN |
denotes |
trapped |
T9507 |
6546-6552 |
NN |
denotes |
RanBP2 |
T9506 |
6553-6557 |
NNS |
denotes |
mice |
T9508 |
6557-6558 |
. |
denotes |
. |
T9509 |
6558-6897 |
sentence |
denotes |
A feeder-independent mouse ES cell line derived from the 129P2/OlaHsd strain and harboring a disrupted RanBP2 locus by insertion mutagenesis with the gene trap vector, pGT0pfs [67,68] (generated by W. Skarnes), was obtained from the Mutant Mouse Regional Resource Center (University of California Davis, Davis, California, United States). |
T9510 |
6559-6560 |
DT |
denotes |
A |
T9512 |
6561-6567 |
NN |
denotes |
feeder |
T9514 |
6567-6568 |
HYPH |
denotes |
- |
T9513 |
6568-6579 |
JJ |
denotes |
independent |
T9515 |
6580-6585 |
NN |
denotes |
mouse |
T9516 |
6586-6588 |
NN |
denotes |
ES |
T9517 |
6589-6593 |
NN |
denotes |
cell |
T9511 |
6594-6598 |
NN |
denotes |
line |
T9519 |
6599-6606 |
VBN |
denotes |
derived |
T9520 |
6607-6611 |
IN |
denotes |
from |
T9521 |
6612-6615 |
DT |
denotes |
the |
T9523 |
6616-6621 |
NN |
denotes |
129P2 |
T9525 |
6621-6622 |
HYPH |
denotes |
/ |
T9524 |
6622-6628 |
NN |
denotes |
OlaHsd |
T9522 |
6629-6635 |
NN |
denotes |
strain |
T9526 |
6636-6639 |
CC |
denotes |
and |
T9527 |
6640-6649 |
VBG |
denotes |
harboring |
T9528 |
6650-6651 |
DT |
denotes |
a |
T9530 |
6652-6661 |
VBN |
denotes |
disrupted |
T9531 |
6662-6668 |
NN |
denotes |
RanBP2 |
T9529 |
6669-6674 |
NN |
denotes |
locus |
T9532 |
6675-6677 |
IN |
denotes |
by |
T9533 |
6678-6687 |
NN |
denotes |
insertion |
T9534 |
6688-6699 |
NN |
denotes |
mutagenesis |
T9535 |
6700-6704 |
IN |
denotes |
with |
T9536 |
6705-6708 |
DT |
denotes |
the |
T9538 |
6709-6713 |
NN |
denotes |
gene |
T9539 |
6714-6718 |
NN |
denotes |
trap |
T9537 |
6719-6725 |
NN |
denotes |
vector |
T9540 |
6725-6727 |
, |
denotes |
, |
T9541 |
6727-6734 |
NN |
denotes |
pGT0pfs |
T9542 |
6735-6736 |
-LRB- |
denotes |
[ |
T9544 |
6736-6738 |
CD |
denotes |
67 |
T9545 |
6738-6739 |
, |
denotes |
, |
T9543 |
6739-6741 |
CD |
denotes |
68 |
T9546 |
6741-6742 |
-RRB- |
denotes |
] |
T9547 |
6743-6744 |
-LRB- |
denotes |
( |
T9548 |
6744-6753 |
VBN |
denotes |
generated |
T9549 |
6754-6756 |
IN |
denotes |
by |
T9550 |
6757-6759 |
NNP |
denotes |
W. |
T9551 |
6760-6767 |
NNP |
denotes |
Skarnes |
T9552 |
6767-6768 |
-RRB- |
denotes |
) |
T9553 |
6768-6770 |
, |
denotes |
, |
T9554 |
6770-6773 |
VBD |
denotes |
was |
T9518 |
6774-6782 |
VBN |
denotes |
obtained |
T9555 |
6783-6787 |
IN |
denotes |
from |
T9556 |
6788-6791 |
DT |
denotes |
the |
T9558 |
6792-6798 |
NNP |
denotes |
Mutant |
T9559 |
6799-6804 |
NNP |
denotes |
Mouse |
T9560 |
6805-6813 |
NNP |
denotes |
Regional |
T9561 |
6814-6822 |
NNP |
denotes |
Resource |
T9557 |
6823-6829 |
NNP |
denotes |
Center |
T9562 |
6830-6831 |
-LRB- |
denotes |
( |
T9563 |
6831-6841 |
NNP |
denotes |
University |
T9564 |
6842-6844 |
IN |
denotes |
of |
T9565 |
6845-6855 |
NNP |
denotes |
California |
T9566 |
6856-6861 |
NNP |
denotes |
Davis |
T9567 |
6861-6863 |
, |
denotes |
, |
T9568 |
6863-6868 |
NNP |
denotes |
Davis |
T9569 |
6868-6870 |
, |
denotes |
, |
T9570 |
6870-6880 |
NNP |
denotes |
California |
T9571 |
6880-6882 |
, |
denotes |
, |
T9572 |
6882-6888 |
NNP |
denotes |
United |
T9573 |
6889-6895 |
NNP |
denotes |
States |
T9574 |
6895-6896 |
-RRB- |
denotes |
) |
T9575 |
6896-6897 |
. |
denotes |
. |
T9576 |
6897-7127 |
sentence |
denotes |
The gene trap vector contains a promoterless neo-lacZ fusion gene with a splice acceptor site at the 5′ end followed by an internal ribosome entry site (IRES) and the human placental alkaline phosphatase (PLAP) reporter cassette. |
T9577 |
6898-6901 |
DT |
denotes |
The |
T9579 |
6902-6906 |
NN |
denotes |
gene |
T9580 |
6907-6911 |
NN |
denotes |
trap |
T9578 |
6912-6918 |
NN |
denotes |
vector |
T9581 |
6919-6927 |
VBZ |
denotes |
contains |
T9582 |
6928-6929 |
DT |
denotes |
a |
T9584 |
6930-6942 |
JJ |
denotes |
promoterless |
T9585 |
6943-6951 |
NN |
denotes |
neo-lacZ |
T9586 |
6952-6958 |
NN |
denotes |
fusion |
T9583 |
6959-6963 |
NN |
denotes |
gene |
T9587 |
6964-6968 |
IN |
denotes |
with |
T9588 |
6969-6970 |
DT |
denotes |
a |
T9590 |
6971-6977 |
NN |
denotes |
splice |
T9591 |
6978-6986 |
NN |
denotes |
acceptor |
T9589 |
6987-6991 |
NN |
denotes |
site |
T9592 |
6992-6994 |
IN |
denotes |
at |
T9593 |
6995-6998 |
DT |
denotes |
the |
T9595 |
6999-7000 |
CD |
denotes |
5 |
T9596 |
7000-7001 |
SYM |
denotes |
′ |
T9594 |
7002-7005 |
NN |
denotes |
end |
T9597 |
7006-7014 |
VBN |
denotes |
followed |
T9598 |
7015-7017 |
IN |
denotes |
by |
T9599 |
7018-7020 |
DT |
denotes |
an |
T9601 |
7021-7029 |
JJ |
denotes |
internal |
T9602 |
7030-7038 |
NN |
denotes |
ribosome |
T9603 |
7039-7044 |
NN |
denotes |
entry |
T9600 |
7045-7049 |
NN |
denotes |
site |
T9604 |
7050-7051 |
-LRB- |
denotes |
( |
T9605 |
7051-7055 |
NN |
denotes |
IRES |
T9606 |
7055-7056 |
-RRB- |
denotes |
) |
T9607 |
7057-7060 |
CC |
denotes |
and |
T9608 |
7061-7064 |
DT |
denotes |
the |
T9610 |
7065-7070 |
JJ |
denotes |
human |
T9611 |
7071-7080 |
JJ |
denotes |
placental |
T9613 |
7081-7089 |
NN |
denotes |
alkaline |
T9612 |
7090-7101 |
NN |
denotes |
phosphatase |
T9614 |
7102-7103 |
-LRB- |
denotes |
( |
T9615 |
7103-7107 |
NN |
denotes |
PLAP |
T9616 |
7107-7108 |
-RRB- |
denotes |
) |
T9617 |
7109-7117 |
NN |
denotes |
reporter |
T9609 |
7118-7126 |
NN |
denotes |
cassette |
T9618 |
7126-7127 |
. |
denotes |
. |
T9619 |
7127-7231 |
sentence |
denotes |
The RanBP2 129Ola ES line was injected into C57BL/6J blastocysts and four male chimeras were generated. |
T9620 |
7128-7131 |
DT |
denotes |
The |
T9622 |
7132-7138 |
NN |
denotes |
RanBP2 |
T9623 |
7139-7145 |
NN |
denotes |
129Ola |
T9624 |
7146-7148 |
NN |
denotes |
ES |
T9621 |
7149-7153 |
NN |
denotes |
line |
T9626 |
7154-7157 |
VBD |
denotes |
was |
T9625 |
7158-7166 |
VBN |
denotes |
injected |
T9627 |
7167-7171 |
IN |
denotes |
into |
T9628 |
7172-7177 |
NN |
denotes |
C57BL |
T9630 |
7177-7178 |
HYPH |
denotes |
/ |
T9629 |
7178-7180 |
NN |
denotes |
6J |
T9631 |
7181-7192 |
NNS |
denotes |
blastocysts |
T9632 |
7193-7196 |
CC |
denotes |
and |
T9633 |
7197-7201 |
CD |
denotes |
four |
T9635 |
7202-7206 |
JJ |
denotes |
male |
T9634 |
7207-7215 |
NNS |
denotes |
chimeras |
T9637 |
7216-7220 |
VBD |
denotes |
were |
T9636 |
7221-7230 |
VBN |
denotes |
generated |
T9638 |
7230-7231 |
. |
denotes |
. |
T9639 |
7231-7389 |
sentence |
denotes |
These were bred into 129P2/OlaHsd (Harlan, Indianapolis, Indiana, United States) and C57BL/6J (Jackson Laboratory, Bar Harbor, Maine, United States) females. |
T9640 |
7232-7237 |
DT |
denotes |
These |
T9642 |
7238-7242 |
VBD |
denotes |
were |
T9641 |
7243-7247 |
VBN |
denotes |
bred |
T9643 |
7248-7252 |
IN |
denotes |
into |
T9644 |
7253-7258 |
NN |
denotes |
129P2 |
T9646 |
7258-7259 |
HYPH |
denotes |
/ |
T9645 |
7259-7265 |
NN |
denotes |
OlaHsd |
T9648 |
7266-7267 |
-LRB- |
denotes |
( |
T9649 |
7267-7273 |
NNP |
denotes |
Harlan |
T9650 |
7273-7275 |
, |
denotes |
, |
T9651 |
7275-7287 |
NNP |
denotes |
Indianapolis |
T9652 |
7287-7289 |
, |
denotes |
, |
T9653 |
7289-7296 |
NNP |
denotes |
Indiana |
T9654 |
7296-7298 |
, |
denotes |
, |
T9655 |
7298-7304 |
NNP |
denotes |
United |
T9656 |
7305-7311 |
NNP |
denotes |
States |
T9657 |
7311-7312 |
-RRB- |
denotes |
) |
T9658 |
7313-7316 |
CC |
denotes |
and |
T9659 |
7317-7322 |
NN |
denotes |
C57BL |
T9661 |
7322-7323 |
HYPH |
denotes |
/ |
T9660 |
7323-7325 |
NN |
denotes |
6J |
T9662 |
7326-7327 |
-LRB- |
denotes |
( |
T9664 |
7327-7334 |
NNP |
denotes |
Jackson |
T9663 |
7335-7345 |
NNP |
denotes |
Laboratory |
T9665 |
7345-7347 |
, |
denotes |
, |
T9666 |
7347-7350 |
NNP |
denotes |
Bar |
T9667 |
7351-7357 |
NNP |
denotes |
Harbor |
T9668 |
7357-7359 |
, |
denotes |
, |
T9669 |
7359-7364 |
NNP |
denotes |
Maine |
T9670 |
7364-7366 |
, |
denotes |
, |
T9671 |
7366-7372 |
NNP |
denotes |
United |
T9672 |
7373-7379 |
NNP |
denotes |
States |
T9673 |
7379-7380 |
-RRB- |
denotes |
) |
T9647 |
7381-7388 |
NNS |
denotes |
females |
T9674 |
7388-7389 |
. |
denotes |
. |
T9675 |
7389-7605 |
sentence |
denotes |
The two resulting and independent F1s lines, with 129P2/OlaHsd inbred and 129P2/OlaHsd/C57BL/6J mixed genetic backgrounds, were tested for germline transmission by PCR and Southern blot analysis of tail genomic DNA. |
T9676 |
7390-7393 |
DT |
denotes |
The |
T9678 |
7394-7397 |
CD |
denotes |
two |
T9679 |
7398-7407 |
VBG |
denotes |
resulting |
T9680 |
7408-7411 |
CC |
denotes |
and |
T9681 |
7412-7423 |
JJ |
denotes |
independent |
T9682 |
7424-7427 |
NN |
denotes |
F1s |
T9677 |
7428-7433 |
NNS |
denotes |
lines |
T9684 |
7433-7435 |
, |
denotes |
, |
T9685 |
7435-7439 |
IN |
denotes |
with |
T9686 |
7440-7445 |
NN |
denotes |
129P2 |
T9688 |
7445-7446 |
HYPH |
denotes |
/ |
T9687 |
7446-7452 |
NN |
denotes |
OlaHsd |
T9689 |
7453-7459 |
JJ |
denotes |
inbred |
T9690 |
7460-7463 |
CC |
denotes |
and |
T9691 |
7464-7469 |
NN |
denotes |
129P2 |
T9693 |
7469-7470 |
HYPH |
denotes |
/ |
T9694 |
7470-7476 |
NN |
denotes |
OlaHsd |
T9695 |
7476-7477 |
HYPH |
denotes |
/ |
T9696 |
7477-7482 |
NN |
denotes |
C57BL |
T9697 |
7482-7483 |
HYPH |
denotes |
/ |
T9692 |
7483-7485 |
NN |
denotes |
6J |
T9699 |
7486-7491 |
JJ |
denotes |
mixed |
T9700 |
7492-7499 |
JJ |
denotes |
genetic |
T9698 |
7500-7511 |
NNS |
denotes |
backgrounds |
T9701 |
7511-7513 |
, |
denotes |
, |
T9702 |
7513-7517 |
VBD |
denotes |
were |
T9683 |
7518-7524 |
VBN |
denotes |
tested |
T9703 |
7525-7528 |
IN |
denotes |
for |
T9704 |
7529-7537 |
NN |
denotes |
germline |
T9705 |
7538-7550 |
NN |
denotes |
transmission |
T9706 |
7551-7553 |
IN |
denotes |
by |
T9707 |
7554-7557 |
NN |
denotes |
PCR |
T9709 |
7558-7561 |
CC |
denotes |
and |
T9710 |
7562-7570 |
NNP |
denotes |
Southern |
T9711 |
7571-7575 |
NN |
denotes |
blot |
T9708 |
7576-7584 |
NN |
denotes |
analysis |
T9712 |
7585-7587 |
IN |
denotes |
of |
T9713 |
7588-7592 |
NN |
denotes |
tail |
T9715 |
7593-7600 |
JJ |
denotes |
genomic |
T9714 |
7601-7604 |
NN |
denotes |
DNA |
T9716 |
7604-7605 |
. |
denotes |
. |
T9717 |
7605-7960 |
sentence |
denotes |
β-gal and PLAP activities in whole mount embryos and retinal sections were detected, respectively, by incubation with 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside and AP staining buffer (0.1 mg/ml 5-bromo-4-chloro-3-indolyl phosphate, 1 mg/ml nitroblue tetrazolium in 100 mM Tris-HCl (pH 9.5), 100 mM NaCl, 5 mM MgCl2) as described elsewhere [67,68]. |
T9718 |
7606-7607 |
NN |
denotes |
β |
T9720 |
7607-7608 |
HYPH |
denotes |
- |
T9719 |
7608-7611 |
NN |
denotes |
gal |
T9722 |
7612-7615 |
CC |
denotes |
and |
T9723 |
7616-7620 |
NN |
denotes |
PLAP |
T9721 |
7621-7631 |
NNS |
denotes |
activities |
T9725 |
7632-7634 |
IN |
denotes |
in |
T9726 |
7635-7640 |
JJ |
denotes |
whole |
T9727 |
7641-7646 |
NN |
denotes |
mount |
T9728 |
7647-7654 |
NNS |
denotes |
embryos |
T9729 |
7655-7658 |
CC |
denotes |
and |
T9730 |
7659-7666 |
JJ |
denotes |
retinal |
T9731 |
7667-7675 |
NNS |
denotes |
sections |
T9732 |
7676-7680 |
VBD |
denotes |
were |
T9724 |
7681-7689 |
VBN |
denotes |
detected |
T9733 |
7689-7691 |
, |
denotes |
, |
T9734 |
7691-7703 |
RB |
denotes |
respectively |
T9735 |
7703-7705 |
, |
denotes |
, |
T9736 |
7705-7707 |
IN |
denotes |
by |
T9737 |
7708-7718 |
NN |
denotes |
incubation |
T9738 |
7719-7723 |
IN |
denotes |
with |
T9739 |
7724-7725 |
CD |
denotes |
5 |
T9741 |
7725-7726 |
HYPH |
denotes |
- |
T9740 |
7726-7731 |
NN |
denotes |
bromo |
T9743 |
7731-7732 |
HYPH |
denotes |
- |
T9744 |
7732-7733 |
CD |
denotes |
4 |
T9745 |
7733-7734 |
HYPH |
denotes |
- |
T9746 |
7734-7740 |
NN |
denotes |
chloro |
T9747 |
7740-7741 |
HYPH |
denotes |
- |
T9748 |
7741-7742 |
CD |
denotes |
3 |
T9750 |
7742-7743 |
HYPH |
denotes |
- |
T9751 |
7743-7750 |
NN |
denotes |
indolyl |
T9749 |
7751-7752 |
NN |
denotes |
β |
T9752 |
7752-7753 |
HYPH |
denotes |
- |
T9753 |
7753-7754 |
NN |
denotes |
D |
T9754 |
7754-7755 |
HYPH |
denotes |
- |
T9742 |
7755-7772 |
NN |
denotes |
galactopyranoside |
T9756 |
7773-7776 |
CC |
denotes |
and |
T9757 |
7777-7779 |
NN |
denotes |
AP |
T9758 |
7780-7788 |
NN |
denotes |
staining |
T9755 |
7789-7795 |
NN |
denotes |
buffer |
T9759 |
7796-7797 |
-LRB- |
denotes |
( |
T9761 |
7797-7800 |
CD |
denotes |
0.1 |
T9762 |
7801-7803 |
NNS |
denotes |
mg |
T9764 |
7803-7804 |
SYM |
denotes |
/ |
T9765 |
7804-7806 |
NN |
denotes |
ml |
T9766 |
7807-7808 |
CD |
denotes |
5 |
T9768 |
7808-7809 |
HYPH |
denotes |
- |
T9767 |
7809-7814 |
NN |
denotes |
bromo |
T9769 |
7814-7815 |
HYPH |
denotes |
- |
T9770 |
7815-7816 |
CD |
denotes |
4 |
T9772 |
7816-7817 |
HYPH |
denotes |
- |
T9771 |
7817-7823 |
NN |
denotes |
chloro |
T9773 |
7823-7824 |
HYPH |
denotes |
- |
T9774 |
7824-7825 |
CD |
denotes |
3 |
T9775 |
7825-7826 |
HYPH |
denotes |
- |
T9776 |
7826-7833 |
NN |
denotes |
indolyl |
T9763 |
7834-7843 |
NN |
denotes |
phosphate |
T9777 |
7843-7845 |
, |
denotes |
, |
T9778 |
7845-7846 |
CD |
denotes |
1 |
T9779 |
7847-7849 |
NNS |
denotes |
mg |
T9780 |
7849-7850 |
SYM |
denotes |
/ |
T9781 |
7850-7852 |
NN |
denotes |
ml |
T9782 |
7853-7862 |
JJ |
denotes |
nitroblue |
T9760 |
7863-7874 |
NN |
denotes |
tetrazolium |
T9783 |
7875-7877 |
IN |
denotes |
in |
T9784 |
7878-7881 |
CD |
denotes |
100 |
T9785 |
7882-7884 |
NNS |
denotes |
mM |
T9787 |
7885-7889 |
NN |
denotes |
Tris |
T9788 |
7889-7890 |
HYPH |
denotes |
- |
T9786 |
7890-7893 |
NN |
denotes |
HCl |
T9789 |
7894-7895 |
-LRB- |
denotes |
( |
T9790 |
7895-7897 |
NN |
denotes |
pH |
T9791 |
7898-7901 |
CD |
denotes |
9.5 |
T9792 |
7901-7902 |
-RRB- |
denotes |
) |
T9793 |
7902-7904 |
, |
denotes |
, |
T9794 |
7904-7907 |
CD |
denotes |
100 |
T9795 |
7908-7910 |
NNS |
denotes |
mM |
T9796 |
7911-7915 |
NN |
denotes |
NaCl |
T9797 |
7915-7917 |
, |
denotes |
, |
T9798 |
7917-7918 |
CD |
denotes |
5 |
T9799 |
7919-7921 |
NNS |
denotes |
mM |
T9800 |
7922-7927 |
NN |
denotes |
MgCl2 |
T9801 |
7927-7928 |
-RRB- |
denotes |
) |
T9802 |
7929-7931 |
IN |
denotes |
as |
T9803 |
7932-7941 |
VBN |
denotes |
described |
T9804 |
7942-7951 |
RB |
denotes |
elsewhere |
T9805 |
7952-7953 |
-LRB- |
denotes |
[ |
T9807 |
7953-7955 |
CD |
denotes |
67 |
T9808 |
7955-7956 |
, |
denotes |
, |
T9806 |
7956-7958 |
CD |
denotes |
68 |
T9809 |
7958-7959 |
-RRB- |
denotes |
] |
T9810 |
7959-7960 |
. |
denotes |
. |
T9862 |
7962-7969 |
NNP |
denotes |
Western |
T9863 |
7970-7974 |
NN |
denotes |
blot |
T9864 |
7975-7978 |
CC |
denotes |
and |
T9865 |
7979-7991 |
NN |
denotes |
quantitation |
T9866 |
7992-8000 |
NN |
denotes |
analysis |
T9867 |
8001-8003 |
IN |
denotes |
of |
T9868 |
8004-8011 |
NN |
denotes |
protein |
T9869 |
8012-8022 |
NN |
denotes |
expression |
T9870 |
8023-8026 |
CC |
denotes |
and |
T9871 |
8027-8030 |
NN |
denotes |
ATP |
T9872 |
8031-8037 |
NNS |
denotes |
levels |
T9873 |
8037-8038 |
. |
denotes |
. |
T9874 |
8038-8155 |
sentence |
denotes |
Homogenates and detergent solubilized extracts of tissues and immunoblots were prepared as described elsewhere [69]. |
T9875 |
8039-8050 |
NNS |
denotes |
Homogenates |
T9877 |
8051-8054 |
CC |
denotes |
and |
T9878 |
8055-8064 |
NN |
denotes |
detergent |
T9879 |
8065-8076 |
VBN |
denotes |
solubilized |
T9880 |
8077-8085 |
NNS |
denotes |
extracts |
T9881 |
8086-8088 |
IN |
denotes |
of |
T9882 |
8089-8096 |
NNS |
denotes |
tissues |
T9883 |
8097-8100 |
CC |
denotes |
and |
T9884 |
8101-8112 |
NNS |
denotes |
immunoblots |
T9885 |
8113-8117 |
VBD |
denotes |
were |
T9876 |
8118-8126 |
VBN |
denotes |
prepared |
T9886 |
8127-8129 |
IN |
denotes |
as |
T9887 |
8130-8139 |
VBN |
denotes |
described |
T9888 |
8140-8149 |
RB |
denotes |
elsewhere |
T9889 |
8150-8151 |
-LRB- |
denotes |
[ |
T9890 |
8151-8153 |
CD |
denotes |
69 |
T9891 |
8153-8154 |
-RRB- |
denotes |
] |
T9892 |
8154-8155 |
. |
denotes |
. |
T9893 |
8155-8353 |
sentence |
denotes |
Quantitation of immunoblot bands calibrated against internal marker bands, such as mHsp70 and Nup153, was carried out with Gel Pro Analyzer (MediaCybernetics, San Diego, California, United States). |
T9894 |
8156-8168 |
NN |
denotes |
Quantitation |
T9896 |
8169-8171 |
IN |
denotes |
of |
T9897 |
8172-8182 |
NN |
denotes |
immunoblot |
T9898 |
8183-8188 |
NNS |
denotes |
bands |
T9899 |
8189-8199 |
VBN |
denotes |
calibrated |
T9900 |
8200-8207 |
IN |
denotes |
against |
T9901 |
8208-8216 |
JJ |
denotes |
internal |
T9903 |
8217-8223 |
NN |
denotes |
marker |
T9902 |
8224-8229 |
NNS |
denotes |
bands |
T9904 |
8229-8231 |
, |
denotes |
, |
T9905 |
8231-8235 |
JJ |
denotes |
such |
T9906 |
8236-8238 |
IN |
denotes |
as |
T9907 |
8239-8245 |
NN |
denotes |
mHsp70 |
T9908 |
8246-8249 |
CC |
denotes |
and |
T9909 |
8250-8256 |
NN |
denotes |
Nup153 |
T9910 |
8256-8258 |
, |
denotes |
, |
T9911 |
8258-8261 |
VBD |
denotes |
was |
T9895 |
8262-8269 |
VBN |
denotes |
carried |
T9912 |
8270-8273 |
RP |
denotes |
out |
T9913 |
8274-8278 |
IN |
denotes |
with |
T9914 |
8279-8282 |
NNP |
denotes |
Gel |
T9916 |
8283-8286 |
NNP |
denotes |
Pro |
T9915 |
8287-8295 |
NNP |
denotes |
Analyzer |
T9917 |
8296-8297 |
-LRB- |
denotes |
( |
T9918 |
8297-8313 |
NNPS |
denotes |
MediaCybernetics |
T9919 |
8313-8315 |
, |
denotes |
, |
T9920 |
8315-8318 |
NNP |
denotes |
San |
T9921 |
8319-8324 |
NNP |
denotes |
Diego |
T9922 |
8324-8326 |
, |
denotes |
, |
T9923 |
8326-8336 |
NNP |
denotes |
California |
T9924 |
8336-8338 |
, |
denotes |
, |
T9925 |
8338-8344 |
NNP |
denotes |
United |
T9926 |
8345-8351 |
NNP |
denotes |
States |
T9927 |
8351-8352 |
-RRB- |
denotes |
) |
T9928 |
8352-8353 |
. |
denotes |
. |
T9929 |
8353-8569 |
sentence |
denotes |
For determination of ATP levels, freshly dissected and flash-frozen tissues were homogenized in 2.5% trichloroacetic acid (TCA), neutralized, and diluted to a final concentration of 0.1% with Tris-Acetate (pH 7.75). |
T9930 |
8354-8357 |
IN |
denotes |
For |
T9932 |
8358-8371 |
NN |
denotes |
determination |
T9933 |
8372-8374 |
IN |
denotes |
of |
T9934 |
8375-8378 |
NN |
denotes |
ATP |
T9935 |
8379-8385 |
NNS |
denotes |
levels |
T9936 |
8385-8387 |
, |
denotes |
, |
T9937 |
8387-8394 |
RB |
denotes |
freshly |
T9938 |
8395-8404 |
VBN |
denotes |
dissected |
T9940 |
8405-8408 |
CC |
denotes |
and |
T9941 |
8409-8414 |
NN |
denotes |
flash |
T9943 |
8414-8415 |
HYPH |
denotes |
- |
T9942 |
8415-8421 |
VBN |
denotes |
frozen |
T9939 |
8422-8429 |
NNS |
denotes |
tissues |
T9944 |
8430-8434 |
VBD |
denotes |
were |
T9931 |
8435-8446 |
VBN |
denotes |
homogenized |
T9945 |
8447-8449 |
IN |
denotes |
in |
T9946 |
8450-8453 |
CD |
denotes |
2.5 |
T9947 |
8453-8454 |
NN |
denotes |
% |
T9948 |
8455-8470 |
JJ |
denotes |
trichloroacetic |
T9949 |
8471-8475 |
NN |
denotes |
acid |
T9950 |
8476-8477 |
-LRB- |
denotes |
( |
T9951 |
8477-8480 |
NN |
denotes |
TCA |
T9952 |
8480-8481 |
-RRB- |
denotes |
) |
T9953 |
8481-8483 |
, |
denotes |
, |
T9954 |
8483-8494 |
VBN |
denotes |
neutralized |
T9955 |
8494-8496 |
, |
denotes |
, |
T9956 |
8496-8499 |
CC |
denotes |
and |
T9957 |
8500-8507 |
VBD |
denotes |
diluted |
T9958 |
8508-8510 |
IN |
denotes |
to |
T9959 |
8511-8512 |
DT |
denotes |
a |
T9961 |
8513-8518 |
JJ |
denotes |
final |
T9960 |
8519-8532 |
NN |
denotes |
concentration |
T9962 |
8533-8535 |
IN |
denotes |
of |
T9963 |
8536-8539 |
CD |
denotes |
0.1 |
T9964 |
8539-8540 |
NN |
denotes |
% |
T9965 |
8541-8545 |
IN |
denotes |
with |
T9966 |
8546-8550 |
NN |
denotes |
Tris |
T9968 |
8550-8551 |
HYPH |
denotes |
- |
T9967 |
8551-8558 |
NN |
denotes |
Acetate |
T9969 |
8559-8560 |
-LRB- |
denotes |
( |
T9970 |
8560-8562 |
NN |
denotes |
pH |
T9971 |
8563-8567 |
CD |
denotes |
7.75 |
T9972 |
8567-8568 |
-RRB- |
denotes |
) |
T9973 |
8568-8569 |
. |
denotes |
. |
T9974 |
8569-8834 |
sentence |
denotes |
ATP measurements were carried out with the ENLITEN ATP Assay System Bioluminescence Detection Kit for ATP (Promega, Madison, Wisconsin, United States) as per the manufacturer's instruction in SpectraMax-M5 (Molecular Devices, Sunnyvale, California, United States). |
T9975 |
8570-8573 |
NN |
denotes |
ATP |
T9976 |
8574-8586 |
NNS |
denotes |
measurements |
T9978 |
8587-8591 |
VBD |
denotes |
were |
T9977 |
8592-8599 |
VBN |
denotes |
carried |
T9979 |
8600-8603 |
RP |
denotes |
out |
T9980 |
8604-8608 |
IN |
denotes |
with |
T9981 |
8609-8612 |
DT |
denotes |
the |
T9983 |
8613-8620 |
NNP |
denotes |
ENLITEN |
T9984 |
8621-8624 |
NNP |
denotes |
ATP |
T9986 |
8625-8630 |
NNP |
denotes |
Assay |
T9985 |
8631-8637 |
NNP |
denotes |
System |
T9987 |
8638-8653 |
NNP |
denotes |
Bioluminescence |
T9988 |
8654-8663 |
NNP |
denotes |
Detection |
T9982 |
8664-8667 |
NNP |
denotes |
Kit |
T9989 |
8668-8671 |
IN |
denotes |
for |
T9990 |
8672-8675 |
NN |
denotes |
ATP |
T9991 |
8676-8677 |
-LRB- |
denotes |
( |
T9992 |
8677-8684 |
NNP |
denotes |
Promega |
T9993 |
8684-8686 |
, |
denotes |
, |
T9994 |
8686-8693 |
NNP |
denotes |
Madison |
T9995 |
8693-8695 |
, |
denotes |
, |
T9996 |
8695-8704 |
NNP |
denotes |
Wisconsin |
T9997 |
8704-8706 |
, |
denotes |
, |
T9998 |
8706-8712 |
NNP |
denotes |
United |
T9999 |
8713-8719 |
NNP |
denotes |
States |
T10000 |
8719-8720 |
-RRB- |
denotes |
) |
T10001 |
8721-8723 |
IN |
denotes |
as |
T10002 |
8724-8727 |
IN |
denotes |
per |
T10003 |
8728-8731 |
DT |
denotes |
the |
T10004 |
8732-8744 |
NN |
denotes |
manufacturer |
T10006 |
8744-8746 |
POS |
denotes |
's |
T10005 |
8747-8758 |
NN |
denotes |
instruction |
T10007 |
8759-8761 |
IN |
denotes |
in |
T10008 |
8762-8772 |
NNP |
denotes |
SpectraMax |
T10010 |
8772-8773 |
HYPH |
denotes |
- |
T10009 |
8773-8775 |
NNP |
denotes |
M5 |
T10011 |
8776-8777 |
-LRB- |
denotes |
( |
T10013 |
8777-8786 |
NNP |
denotes |
Molecular |
T10012 |
8787-8794 |
NNPS |
denotes |
Devices |
T10014 |
8794-8796 |
, |
denotes |
, |
T10015 |
8796-8805 |
NNP |
denotes |
Sunnyvale |
T10016 |
8805-8807 |
, |
denotes |
, |
T10017 |
8807-8817 |
NNP |
denotes |
California |
T10018 |
8817-8819 |
, |
denotes |
, |
T10019 |
8819-8825 |
NNP |
denotes |
United |
T10020 |
8826-8832 |
NNP |
denotes |
States |
T10021 |
8832-8833 |
-RRB- |
denotes |
) |
T10022 |
8833-8834 |
. |
denotes |
. |
T10023 |
8834-8958 |
sentence |
denotes |
Protein concentration was measured by the BCA method (BioRad, Hercules, California, United States) using BSA as a standard. |
T10024 |
8835-8842 |
NN |
denotes |
Protein |
T10025 |
8843-8856 |
NN |
denotes |
concentration |
T10027 |
8857-8860 |
VBD |
denotes |
was |
T10026 |
8861-8869 |
VBN |
denotes |
measured |
T10028 |
8870-8872 |
IN |
denotes |
by |
T10029 |
8873-8876 |
DT |
denotes |
the |
T10031 |
8877-8880 |
NN |
denotes |
BCA |
T10030 |
8881-8887 |
NN |
denotes |
method |
T10032 |
8888-8889 |
-LRB- |
denotes |
( |
T10033 |
8889-8895 |
NNP |
denotes |
BioRad |
T10034 |
8895-8897 |
, |
denotes |
, |
T10035 |
8897-8905 |
NNP |
denotes |
Hercules |
T10036 |
8905-8907 |
, |
denotes |
, |
T10037 |
8907-8917 |
NNP |
denotes |
California |
T10038 |
8917-8919 |
, |
denotes |
, |
T10039 |
8919-8925 |
NNP |
denotes |
United |
T10040 |
8926-8932 |
NNP |
denotes |
States |
T10041 |
8932-8933 |
-RRB- |
denotes |
) |
T10042 |
8934-8939 |
VBG |
denotes |
using |
T10043 |
8940-8943 |
NN |
denotes |
BSA |
T10044 |
8944-8946 |
IN |
denotes |
as |
T10045 |
8947-8948 |
DT |
denotes |
a |
T10046 |
8949-8957 |
NN |
denotes |
standard |
T10047 |
8957-8958 |
. |
denotes |
. |
T10131 |
8960-8969 |
JJ |
denotes |
Metabolic |
T10132 |
8970-8976 |
NNS |
denotes |
assays |
T10133 |
8976-8977 |
. |
denotes |
. |
T10134 |
8977-9057 |
sentence |
denotes |
Two groups of male mice were kept under separate diet since gestation or birth. |
T10135 |
8978-8981 |
CD |
denotes |
Two |
T10136 |
8982-8988 |
NNS |
denotes |
groups |
T10138 |
8989-8991 |
IN |
denotes |
of |
T10139 |
8992-8996 |
JJ |
denotes |
male |
T10140 |
8997-9001 |
NNS |
denotes |
mice |
T10141 |
9002-9006 |
VBD |
denotes |
were |
T10137 |
9007-9011 |
VBN |
denotes |
kept |
T10142 |
9012-9017 |
IN |
denotes |
under |
T10143 |
9018-9026 |
JJ |
denotes |
separate |
T10144 |
9027-9031 |
NN |
denotes |
diet |
T10145 |
9032-9037 |
IN |
denotes |
since |
T10146 |
9038-9047 |
NN |
denotes |
gestation |
T10147 |
9048-9050 |
CC |
denotes |
or |
T10148 |
9051-9056 |
NN |
denotes |
birth |
T10149 |
9056-9057 |
. |
denotes |
. |
T10150 |
9057-9273 |
sentence |
denotes |
One group was fed with a normal chow diet (PicoLab Rodent Diet 20 −5053; LabDiet, Richmond, Indiana, United States), while the other was placed on a higher energy and fat diet (PicoLab Mouse Diet 20 −5058; LabDiet). |
T10151 |
9058-9061 |
CD |
denotes |
One |
T10152 |
9062-9067 |
NN |
denotes |
group |
T10154 |
9068-9071 |
VBD |
denotes |
was |
T10153 |
9072-9075 |
VBN |
denotes |
fed |
T10155 |
9076-9080 |
IN |
denotes |
with |
T10156 |
9081-9082 |
DT |
denotes |
a |
T10158 |
9083-9089 |
JJ |
denotes |
normal |
T10159 |
9090-9094 |
NN |
denotes |
chow |
T10157 |
9095-9099 |
NN |
denotes |
diet |
T10160 |
9100-9101 |
-LRB- |
denotes |
( |
T10161 |
9101-9108 |
NNP |
denotes |
PicoLab |
T10163 |
9109-9115 |
NNP |
denotes |
Rodent |
T10162 |
9116-9120 |
NNP |
denotes |
Diet |
T10164 |
9121-9123 |
CD |
denotes |
20 |
T10165 |
9124-9125 |
HYPH |
denotes |
− |
T10166 |
9125-9129 |
CD |
denotes |
5053 |
T10167 |
9129-9130 |
: |
denotes |
; |
T10168 |
9131-9138 |
NNP |
denotes |
LabDiet |
T10169 |
9138-9140 |
, |
denotes |
, |
T10170 |
9140-9148 |
NNP |
denotes |
Richmond |
T10171 |
9148-9150 |
, |
denotes |
, |
T10172 |
9150-9157 |
NNP |
denotes |
Indiana |
T10173 |
9157-9159 |
, |
denotes |
, |
T10174 |
9159-9165 |
NNP |
denotes |
United |
T10175 |
9166-9172 |
NNP |
denotes |
States |
T10176 |
9172-9173 |
-RRB- |
denotes |
) |
T10177 |
9173-9175 |
, |
denotes |
, |
T10178 |
9175-9180 |
IN |
denotes |
while |
T10180 |
9181-9184 |
DT |
denotes |
the |
T10181 |
9185-9190 |
NN |
denotes |
other |
T10182 |
9191-9194 |
VBD |
denotes |
was |
T10179 |
9195-9201 |
VBN |
denotes |
placed |
T10183 |
9202-9204 |
IN |
denotes |
on |
T10184 |
9205-9206 |
DT |
denotes |
a |
T10186 |
9207-9213 |
JJR |
denotes |
higher |
T10187 |
9214-9220 |
NN |
denotes |
energy |
T10188 |
9221-9224 |
CC |
denotes |
and |
T10189 |
9225-9228 |
NN |
denotes |
fat |
T10185 |
9229-9233 |
NN |
denotes |
diet |
T10190 |
9234-9235 |
-LRB- |
denotes |
( |
T10191 |
9235-9242 |
NNP |
denotes |
PicoLab |
T10193 |
9243-9248 |
NNP |
denotes |
Mouse |
T10192 |
9249-9253 |
NNP |
denotes |
Diet |
T10194 |
9254-9256 |
CD |
denotes |
20 |
T10195 |
9257-9258 |
HYPH |
denotes |
− |
T10196 |
9258-9262 |
CD |
denotes |
5058 |
T10197 |
9262-9263 |
: |
denotes |
; |
T10198 |
9264-9271 |
NNP |
denotes |
LabDiet |
T10199 |
9271-9272 |
-RRB- |
denotes |
) |
T10200 |
9272-9273 |
. |
denotes |
. |
T10201 |
9273-9405 |
sentence |
denotes |
Glucose tolerance test was performed on overnight-fasted mice injected intraperitoneally with 2 g of glucose per kg of body weight. |
T10202 |
9274-9281 |
NN |
denotes |
Glucose |
T10203 |
9282-9291 |
NN |
denotes |
tolerance |
T10204 |
9292-9296 |
NN |
denotes |
test |
T10206 |
9297-9300 |
VBD |
denotes |
was |
T10205 |
9301-9310 |
VBN |
denotes |
performed |
T10207 |
9311-9313 |
IN |
denotes |
on |
T10208 |
9314-9323 |
JJ |
denotes |
overnight |
T10210 |
9323-9324 |
HYPH |
denotes |
- |
T10209 |
9324-9330 |
VBN |
denotes |
fasted |
T10211 |
9331-9335 |
NNS |
denotes |
mice |
T10212 |
9336-9344 |
VBN |
denotes |
injected |
T10213 |
9345-9362 |
RB |
denotes |
intraperitoneally |
T10214 |
9363-9367 |
IN |
denotes |
with |
T10215 |
9368-9369 |
CD |
denotes |
2 |
T10216 |
9370-9371 |
NNS |
denotes |
g |
T10217 |
9372-9374 |
IN |
denotes |
of |
T10218 |
9375-9382 |
NN |
denotes |
glucose |
T10219 |
9383-9386 |
IN |
denotes |
per |
T10220 |
9387-9389 |
NN |
denotes |
kg |
T10221 |
9390-9392 |
IN |
denotes |
of |
T10222 |
9393-9397 |
NN |
denotes |
body |
T10223 |
9398-9404 |
NN |
denotes |
weight |
T10224 |
9404-9405 |
. |
denotes |
. |
T10225 |
9405-9602 |
sentence |
denotes |
Venous blood glucose values were determined at immediately before (0 min), and 15, 30, 60, and 120 min after the injection with an automatic glucose monitor instrument (One Touch Ultra, Lifescan). |
T10226 |
9406-9412 |
JJ |
denotes |
Venous |
T10228 |
9413-9418 |
NN |
denotes |
blood |
T10229 |
9419-9426 |
NN |
denotes |
glucose |
T10227 |
9427-9433 |
NNS |
denotes |
values |
T10231 |
9434-9438 |
VBD |
denotes |
were |
T10230 |
9439-9449 |
VBN |
denotes |
determined |
T10232 |
9450-9452 |
IN |
denotes |
at |
T10233 |
9453-9464 |
RB |
denotes |
immediately |
T10234 |
9465-9471 |
IN |
denotes |
before |
T10235 |
9472-9473 |
-LRB- |
denotes |
( |
T10237 |
9473-9474 |
CD |
denotes |
0 |
T10236 |
9475-9478 |
NNS |
denotes |
min |
T10238 |
9478-9479 |
-RRB- |
denotes |
) |
T10239 |
9479-9481 |
, |
denotes |
, |
T10240 |
9481-9484 |
CC |
denotes |
and |
T10241 |
9485-9487 |
CD |
denotes |
15 |
T10243 |
9487-9489 |
, |
denotes |
, |
T10244 |
9489-9491 |
CD |
denotes |
30 |
T10245 |
9491-9493 |
, |
denotes |
, |
T10246 |
9493-9495 |
CD |
denotes |
60 |
T10247 |
9495-9497 |
, |
denotes |
, |
T10248 |
9497-9500 |
CC |
denotes |
and |
T10249 |
9501-9504 |
CD |
denotes |
120 |
T10242 |
9505-9508 |
NN |
denotes |
min |
T10251 |
9509-9514 |
IN |
denotes |
after |
T10252 |
9515-9518 |
DT |
denotes |
the |
T10250 |
9519-9528 |
NN |
denotes |
injection |
T10253 |
9529-9533 |
IN |
denotes |
with |
T10254 |
9534-9536 |
DT |
denotes |
an |
T10256 |
9537-9546 |
JJ |
denotes |
automatic |
T10257 |
9547-9554 |
NN |
denotes |
glucose |
T10258 |
9555-9562 |
NN |
denotes |
monitor |
T10255 |
9563-9573 |
NN |
denotes |
instrument |
T10259 |
9574-9575 |
-LRB- |
denotes |
( |
T10260 |
9575-9578 |
CD |
denotes |
One |
T10262 |
9579-9584 |
NNP |
denotes |
Touch |
T10261 |
9585-9590 |
NNP |
denotes |
Ultra |
T10263 |
9590-9592 |
, |
denotes |
, |
T10264 |
9592-9600 |
NNP |
denotes |
Lifescan |
T10265 |
9600-9601 |
-RRB- |
denotes |
) |
T10266 |
9601-9602 |
. |
denotes |
. |
T10267 |
9602-9815 |
sentence |
denotes |
Insulin tolerance test was performed the same way as that described for the glucose tolerance test with the exception that human insulin (0.75 U/kg of body mass) was injected intraperitoneally in non-fasted mice. |
T10268 |
9603-9610 |
NN |
denotes |
Insulin |
T10269 |
9611-9620 |
NN |
denotes |
tolerance |
T10270 |
9621-9625 |
NN |
denotes |
test |
T10272 |
9626-9629 |
VBD |
denotes |
was |
T10271 |
9630-9639 |
VBN |
denotes |
performed |
T10273 |
9640-9643 |
DT |
denotes |
the |
T10275 |
9644-9648 |
JJ |
denotes |
same |
T10274 |
9649-9652 |
NN |
denotes |
way |
T10276 |
9653-9655 |
IN |
denotes |
as |
T10277 |
9656-9660 |
DT |
denotes |
that |
T10278 |
9661-9670 |
VBN |
denotes |
described |
T10279 |
9671-9674 |
IN |
denotes |
for |
T10280 |
9675-9678 |
DT |
denotes |
the |
T10282 |
9679-9686 |
NN |
denotes |
glucose |
T10283 |
9687-9696 |
NN |
denotes |
tolerance |
T10281 |
9697-9701 |
NN |
denotes |
test |
T10284 |
9702-9706 |
IN |
denotes |
with |
T10285 |
9707-9710 |
DT |
denotes |
the |
T10286 |
9711-9720 |
NN |
denotes |
exception |
T10287 |
9721-9725 |
IN |
denotes |
that |
T10289 |
9726-9731 |
JJ |
denotes |
human |
T10290 |
9732-9739 |
NN |
denotes |
insulin |
T10291 |
9740-9741 |
-LRB- |
denotes |
( |
T10293 |
9741-9745 |
CD |
denotes |
0.75 |
T10292 |
9746-9747 |
NNS |
denotes |
U |
T10294 |
9747-9748 |
SYM |
denotes |
/ |
T10295 |
9748-9750 |
NN |
denotes |
kg |
T10296 |
9751-9753 |
IN |
denotes |
of |
T10297 |
9754-9758 |
NN |
denotes |
body |
T10298 |
9759-9763 |
NN |
denotes |
mass |
T10299 |
9763-9764 |
-RRB- |
denotes |
) |
T10300 |
9765-9768 |
VBD |
denotes |
was |
T10288 |
9769-9777 |
VBN |
denotes |
injected |
T10301 |
9778-9795 |
RB |
denotes |
intraperitoneally |
T10302 |
9796-9798 |
IN |
denotes |
in |
T10303 |
9799-9809 |
JJ |
denotes |
non-fasted |
T10304 |
9810-9814 |
NNS |
denotes |
mice |
T10305 |
9814-9815 |
. |
denotes |
. |
T10306 |
9815-10047 |
sentence |
denotes |
Pyruvate challenge test was performed the same way as that described for the glucose tolerance test with the exception that pyruvate dissolved in saline (2 g/kg of body mass) was injected intraperitoneally in overnight-fasted mice. |
T10307 |
9816-9824 |
NN |
denotes |
Pyruvate |
T10308 |
9825-9834 |
NN |
denotes |
challenge |
T10309 |
9835-9839 |
NN |
denotes |
test |
T10311 |
9840-9843 |
VBD |
denotes |
was |
T10310 |
9844-9853 |
VBN |
denotes |
performed |
T10312 |
9854-9857 |
DT |
denotes |
the |
T10314 |
9858-9862 |
JJ |
denotes |
same |
T10313 |
9863-9866 |
NN |
denotes |
way |
T10315 |
9867-9869 |
IN |
denotes |
as |
T10316 |
9870-9874 |
DT |
denotes |
that |
T10317 |
9875-9884 |
VBN |
denotes |
described |
T10318 |
9885-9888 |
IN |
denotes |
for |
T10319 |
9889-9892 |
DT |
denotes |
the |
T10321 |
9893-9900 |
NN |
denotes |
glucose |
T10322 |
9901-9910 |
NN |
denotes |
tolerance |
T10320 |
9911-9915 |
NN |
denotes |
test |
T10323 |
9916-9920 |
IN |
denotes |
with |
T10324 |
9921-9924 |
DT |
denotes |
the |
T10325 |
9925-9934 |
NN |
denotes |
exception |
T10326 |
9935-9939 |
IN |
denotes |
that |
T10328 |
9940-9948 |
NN |
denotes |
pyruvate |
T10329 |
9949-9958 |
VBN |
denotes |
dissolved |
T10330 |
9959-9961 |
IN |
denotes |
in |
T10331 |
9962-9968 |
NN |
denotes |
saline |
T10332 |
9969-9970 |
-LRB- |
denotes |
( |
T10334 |
9970-9971 |
CD |
denotes |
2 |
T10333 |
9972-9973 |
NNS |
denotes |
g |
T10335 |
9973-9974 |
SYM |
denotes |
/ |
T10336 |
9974-9976 |
NN |
denotes |
kg |
T10337 |
9977-9979 |
IN |
denotes |
of |
T10338 |
9980-9984 |
NN |
denotes |
body |
T10339 |
9985-9989 |
NN |
denotes |
mass |
T10340 |
9989-9990 |
-RRB- |
denotes |
) |
T10341 |
9991-9994 |
VBD |
denotes |
was |
T10327 |
9995-10003 |
VBN |
denotes |
injected |
T10342 |
10004-10021 |
RB |
denotes |
intraperitoneally |
T10343 |
10022-10024 |
IN |
denotes |
in |
T10344 |
10025-10034 |
JJ |
denotes |
overnight |
T10346 |
10034-10035 |
HYPH |
denotes |
- |
T10345 |
10035-10041 |
VBN |
denotes |
fasted |
T10347 |
10042-10046 |
NNS |
denotes |
mice |
T10348 |
10046-10047 |
. |
denotes |
. |
T10407 |
10049-10068 |
NN |
denotes |
Electroretinography |
T10408 |
10068-10069 |
. |
denotes |
. |
T10409 |
10069-10240 |
sentence |
denotes |
Five ~6-mo-old male RanBP2+/+ and RanBP2+/− mice in 129ola background were kept in dim cyclic light (10 lux) for 2 wk before electroretinographic responses were recorded. |
T10410 |
10070-10074 |
CD |
denotes |
Five |
T10412 |
10075-10076 |
SYM |
denotes |
~ |
T10413 |
10076-10077 |
CD |
denotes |
6 |
T10415 |
10077-10078 |
HYPH |
denotes |
- |
T10414 |
10078-10080 |
NN |
denotes |
mo |
T10417 |
10080-10081 |
HYPH |
denotes |
- |
T10416 |
10081-10084 |
JJ |
denotes |
old |
T10418 |
10085-10089 |
JJ |
denotes |
male |
T10419 |
10090-10096 |
NN |
denotes |
RanBP2 |
T10420 |
10096-10097 |
SYM |
denotes |
+ |
T10421 |
10097-10098 |
HYPH |
denotes |
/ |
T10422 |
10098-10099 |
SYM |
denotes |
+ |
T10423 |
10100-10103 |
CC |
denotes |
and |
T10424 |
10104-10110 |
NN |
denotes |
RanBP2 |
T10425 |
10110-10111 |
SYM |
denotes |
+ |
T10426 |
10111-10112 |
HYPH |
denotes |
/ |
T10427 |
10112-10113 |
SYM |
denotes |
− |
T10411 |
10114-10118 |
NNS |
denotes |
mice |
T10429 |
10119-10121 |
IN |
denotes |
in |
T10430 |
10122-10128 |
NN |
denotes |
129ola |
T10431 |
10129-10139 |
NN |
denotes |
background |
T10432 |
10140-10144 |
VBD |
denotes |
were |
T10428 |
10145-10149 |
VBN |
denotes |
kept |
T10433 |
10150-10152 |
IN |
denotes |
in |
T10434 |
10153-10156 |
NN |
denotes |
dim |
T10436 |
10157-10163 |
JJ |
denotes |
cyclic |
T10435 |
10164-10169 |
NN |
denotes |
light |
T10437 |
10170-10171 |
-LRB- |
denotes |
( |
T10439 |
10171-10173 |
CD |
denotes |
10 |
T10438 |
10174-10177 |
NN |
denotes |
lux |
T10440 |
10177-10178 |
-RRB- |
denotes |
) |
T10441 |
10179-10182 |
IN |
denotes |
for |
T10442 |
10183-10184 |
CD |
denotes |
2 |
T10443 |
10185-10187 |
NNS |
denotes |
wk |
T10444 |
10188-10194 |
IN |
denotes |
before |
T10446 |
10195-10215 |
JJ |
denotes |
electroretinographic |
T10447 |
10216-10225 |
NNS |
denotes |
responses |
T10448 |
10226-10230 |
VBD |
denotes |
were |
T10445 |
10231-10239 |
VBN |
denotes |
recorded |
T10449 |
10239-10240 |
. |
denotes |
. |
T10450 |
10240-10334 |
sentence |
denotes |
The mice were anesthetized intraperitoneally with ketamine (80 mg/kg) and xylazine (4 mg/kg). |
T10451 |
10241-10244 |
DT |
denotes |
The |
T10452 |
10245-10249 |
NNS |
denotes |
mice |
T10454 |
10250-10254 |
VBD |
denotes |
were |
T10453 |
10255-10267 |
VBN |
denotes |
anesthetized |
T10455 |
10268-10285 |
RB |
denotes |
intraperitoneally |
T10456 |
10286-10290 |
IN |
denotes |
with |
T10457 |
10291-10299 |
NN |
denotes |
ketamine |
T10458 |
10300-10301 |
-LRB- |
denotes |
( |
T10460 |
10301-10303 |
CD |
denotes |
80 |
T10459 |
10304-10306 |
NNS |
denotes |
mg |
T10461 |
10306-10307 |
SYM |
denotes |
/ |
T10462 |
10307-10309 |
NN |
denotes |
kg |
T10463 |
10309-10310 |
-RRB- |
denotes |
) |
T10464 |
10311-10314 |
CC |
denotes |
and |
T10465 |
10315-10323 |
NN |
denotes |
xylazine |
T10466 |
10324-10325 |
-LRB- |
denotes |
( |
T10468 |
10325-10326 |
CD |
denotes |
4 |
T10467 |
10327-10329 |
NNS |
denotes |
mg |
T10469 |
10329-10330 |
SYM |
denotes |
/ |
T10470 |
10330-10332 |
NN |
denotes |
kg |
T10471 |
10332-10333 |
-RRB- |
denotes |
) |
T10472 |
10333-10334 |
. |
denotes |
. |
T10473 |
10334-10426 |
sentence |
denotes |
Body temperature was maintained with a heating pad at 37.5 ± 1 °C throughout the recording. |
T10474 |
10335-10339 |
NN |
denotes |
Body |
T10475 |
10340-10351 |
NN |
denotes |
temperature |
T10477 |
10352-10355 |
VBD |
denotes |
was |
T10476 |
10356-10366 |
VBN |
denotes |
maintained |
T10478 |
10367-10371 |
IN |
denotes |
with |
T10479 |
10372-10373 |
DT |
denotes |
a |
T10481 |
10374-10381 |
NN |
denotes |
heating |
T10480 |
10382-10385 |
NN |
denotes |
pad |
T10482 |
10386-10388 |
IN |
denotes |
at |
T10483 |
10389-10393 |
CD |
denotes |
37.5 |
T10485 |
10394-10395 |
SYM |
denotes |
± |
T10484 |
10396-10397 |
CD |
denotes |
1 |
T10486 |
10398-10400 |
NN |
denotes |
°C |
T10487 |
10401-10411 |
IN |
denotes |
throughout |
T10488 |
10412-10415 |
DT |
denotes |
the |
T10489 |
10416-10425 |
NN |
denotes |
recording |
T10490 |
10425-10426 |
. |
denotes |
. |
T10491 |
10426-10550 |
sentence |
denotes |
The pupils were fully dilated with 0.5% tropicamide and 2.5% phenylephrine HCl, and both eyes were recorded simultaneously. |
T10492 |
10427-10430 |
DT |
denotes |
The |
T10493 |
10431-10437 |
NNS |
denotes |
pupils |
T10495 |
10438-10442 |
VBD |
denotes |
were |
T10496 |
10443-10448 |
RB |
denotes |
fully |
T10494 |
10449-10456 |
VBN |
denotes |
dilated |
T10497 |
10457-10461 |
IN |
denotes |
with |
T10498 |
10462-10465 |
CD |
denotes |
0.5 |
T10499 |
10465-10466 |
NN |
denotes |
% |
T10500 |
10467-10478 |
NN |
denotes |
tropicamide |
T10501 |
10479-10482 |
CC |
denotes |
and |
T10502 |
10483-10486 |
CD |
denotes |
2.5 |
T10503 |
10486-10487 |
NN |
denotes |
% |
T10505 |
10488-10501 |
NN |
denotes |
phenylephrine |
T10504 |
10502-10505 |
NN |
denotes |
HCl |
T10506 |
10505-10507 |
, |
denotes |
, |
T10507 |
10507-10510 |
CC |
denotes |
and |
T10508 |
10511-10515 |
DT |
denotes |
both |
T10509 |
10516-10520 |
NNS |
denotes |
eyes |
T10511 |
10521-10525 |
VBD |
denotes |
were |
T10510 |
10526-10534 |
VBN |
denotes |
recorded |
T10512 |
10535-10549 |
RB |
denotes |
simultaneously |
T10513 |
10549-10550 |
. |
denotes |
. |
T10514 |
10550-10667 |
sentence |
denotes |
Corneal gold-wire loops were used as the active electrodes under 0.5% proparacaine hydrochloride topical anesthesia. |
T10515 |
10551-10558 |
JJ |
denotes |
Corneal |
T10517 |
10559-10563 |
JJ |
denotes |
gold |
T10519 |
10563-10564 |
HYPH |
denotes |
- |
T10518 |
10564-10568 |
NN |
denotes |
wire |
T10516 |
10569-10574 |
NNS |
denotes |
loops |
T10521 |
10575-10579 |
VBD |
denotes |
were |
T10520 |
10580-10584 |
VBN |
denotes |
used |
T10522 |
10585-10587 |
IN |
denotes |
as |
T10523 |
10588-10591 |
DT |
denotes |
the |
T10525 |
10592-10598 |
JJ |
denotes |
active |
T10524 |
10599-10609 |
NNS |
denotes |
electrodes |
T10526 |
10610-10615 |
IN |
denotes |
under |
T10527 |
10616-10619 |
CD |
denotes |
0.5 |
T10528 |
10619-10620 |
NN |
denotes |
% |
T10530 |
10621-10633 |
NN |
denotes |
proparacaine |
T10529 |
10634-10647 |
NN |
denotes |
hydrochloride |
T10532 |
10648-10655 |
JJ |
denotes |
topical |
T10531 |
10656-10666 |
NN |
denotes |
anesthesia |
T10533 |
10666-10667 |
. |
denotes |
. |
T10534 |
10667-10757 |
sentence |
denotes |
Gold-wire electrodes placed on the sclera near the limbus served as reference electrodes. |
T10535 |
10668-10672 |
JJ |
denotes |
Gold |
T10537 |
10672-10673 |
HYPH |
denotes |
- |
T10536 |
10673-10677 |
NN |
denotes |
wire |
T10538 |
10678-10688 |
NNS |
denotes |
electrodes |
T10540 |
10689-10695 |
VBN |
denotes |
placed |
T10541 |
10696-10698 |
IN |
denotes |
on |
T10542 |
10699-10702 |
DT |
denotes |
the |
T10543 |
10703-10709 |
NN |
denotes |
sclera |
T10544 |
10710-10714 |
IN |
denotes |
near |
T10545 |
10715-10718 |
DT |
denotes |
the |
T10546 |
10719-10725 |
NN |
denotes |
limbus |
T10539 |
10726-10732 |
VBD |
denotes |
served |
T10547 |
10733-10735 |
IN |
denotes |
as |
T10548 |
10736-10745 |
NN |
denotes |
reference |
T10549 |
10746-10756 |
NNS |
denotes |
electrodes |
T10550 |
10756-10757 |
. |
denotes |
. |
T10551 |
10757-10796 |
sentence |
denotes |
A ground wire was attached to the ear. |
T10552 |
10758-10759 |
DT |
denotes |
A |
T10554 |
10760-10766 |
NN |
denotes |
ground |
T10553 |
10767-10771 |
NN |
denotes |
wire |
T10556 |
10772-10775 |
VBD |
denotes |
was |
T10555 |
10776-10784 |
VBN |
denotes |
attached |
T10557 |
10785-10787 |
IN |
denotes |
to |
T10558 |
10788-10791 |
DT |
denotes |
the |
T10559 |
10792-10795 |
NN |
denotes |
ear |
T10560 |
10795-10796 |
. |
denotes |
. |
T10561 |
10796-10941 |
sentence |
denotes |
Scotopic responses were recorded from threshold to 9 log units of intensity above threshold, amplified, and filtered (5,000 gain, 0.1–1,000 Hz). |
T10562 |
10797-10805 |
JJ |
denotes |
Scotopic |
T10563 |
10806-10815 |
NNS |
denotes |
responses |
T10565 |
10816-10820 |
VBD |
denotes |
were |
T10564 |
10821-10829 |
VBN |
denotes |
recorded |
T10566 |
10830-10834 |
IN |
denotes |
from |
T10567 |
10835-10844 |
NN |
denotes |
threshold |
T10568 |
10845-10847 |
IN |
denotes |
to |
T10569 |
10848-10849 |
CD |
denotes |
9 |
T10571 |
10850-10853 |
NN |
denotes |
log |
T10570 |
10854-10859 |
NNS |
denotes |
units |
T10572 |
10860-10862 |
IN |
denotes |
of |
T10573 |
10863-10872 |
NN |
denotes |
intensity |
T10574 |
10873-10878 |
IN |
denotes |
above |
T10575 |
10879-10888 |
NN |
denotes |
threshold |
T10576 |
10888-10890 |
, |
denotes |
, |
T10577 |
10890-10899 |
VBN |
denotes |
amplified |
T10578 |
10899-10901 |
, |
denotes |
, |
T10579 |
10901-10904 |
CC |
denotes |
and |
T10580 |
10905-10913 |
VBN |
denotes |
filtered |
T10581 |
10914-10915 |
-LRB- |
denotes |
( |
T10583 |
10915-10920 |
CD |
denotes |
5,000 |
T10584 |
10921-10925 |
NN |
denotes |
gain |
T10585 |
10925-10927 |
, |
denotes |
, |
T10586 |
10927-10930 |
CD |
denotes |
0.1 |
T10588 |
10930-10931 |
SYM |
denotes |
– |
T10587 |
10931-10936 |
CD |
denotes |
1,000 |
T10582 |
10937-10939 |
NN |
denotes |
Hz |
T10589 |
10939-10940 |
-RRB- |
denotes |
) |
T10590 |
10940-10941 |
. |
denotes |
. |
T10591 |
10941-11029 |
sentence |
denotes |
For photopic recordings background light at 34 cd/m2 was used to suppress rod function. |
T10592 |
10942-10945 |
IN |
denotes |
For |
T10594 |
10946-10954 |
JJ |
denotes |
photopic |
T10595 |
10955-10965 |
NNS |
denotes |
recordings |
T10596 |
10966-10976 |
NN |
denotes |
background |
T10597 |
10977-10982 |
NN |
denotes |
light |
T10598 |
10983-10985 |
IN |
denotes |
at |
T10599 |
10986-10988 |
CD |
denotes |
34 |
T10600 |
10989-10991 |
NNS |
denotes |
cd |
T10601 |
10991-10992 |
SYM |
denotes |
/ |
T10602 |
10992-10994 |
NN |
denotes |
m2 |
T10603 |
10995-10998 |
VBD |
denotes |
was |
T10593 |
10999-11003 |
VBN |
denotes |
used |
T10604 |
11004-11006 |
TO |
denotes |
to |
T10605 |
11007-11015 |
VB |
denotes |
suppress |
T10606 |
11016-11019 |
NN |
denotes |
rod |
T10607 |
11020-11028 |
NN |
denotes |
function |
T10608 |
11028-11029 |
. |
denotes |
. |
R5072 |
T7787 |
T7788 |
nmod |
Yeast,screening |
R5073 |
T7789 |
T7790 |
nummod |
two,hybrid |
R5074 |
T7790 |
T7788 |
compound |
hybrid,screening |
R5075 |
T7791 |
T7790 |
punct |
-,hybrid |
R5076 |
T7792 |
T7788 |
cc |
and,screening |
R5077 |
T7793 |
T7788 |
conj |
assays,screening |
R5078 |
T7794 |
T7788 |
punct |
.,screening |
R5079 |
T7796 |
T7797 |
det |
The,LD |
R5080 |
T7797 |
T7798 |
nsubjpass |
LD,subcloned |
R5081 |
T7799 |
T7797 |
prep |
of,LD |
R5082 |
T7800 |
T7801 |
amod |
human,RanBP2 |
R5083 |
T7801 |
T7799 |
pobj |
RanBP2,of |
R5084 |
T7802 |
T7803 |
punct |
(,62 |
R5085 |
T7803 |
T7797 |
parataxis |
62,LD |
R5086 |
T7804 |
T7803 |
nmod |
residues,62 |
R5087 |
T7805 |
T7806 |
punct |
–,711 |
R5088 |
T7806 |
T7803 |
prep |
711,62 |
R5089 |
T7807 |
T7803 |
punct |
),62 |
R5090 |
T7808 |
T7798 |
auxpass |
was,subcloned |
R5091 |
T7809 |
T7798 |
prep |
in,subcloned |
R5092 |
T7810 |
T7809 |
punct |
-,in |
R5093 |
T7811 |
T7809 |
pobj |
frame,in |
R5094 |
T7812 |
T7798 |
prep |
into,subcloned |
R5095 |
T7813 |
T7814 |
det |
the,domain |
R5096 |
T7814 |
T7812 |
pobj |
domain,into |
R5097 |
T7815 |
T7814 |
compound |
GAL4,domain |
R5098 |
T7816 |
T7817 |
compound |
DNA,binding |
R5099 |
T7817 |
T7814 |
compound |
binding,domain |
R5100 |
T7818 |
T7817 |
punct |
-,binding |
R5101 |
T7819 |
T7814 |
prep |
of,domain |
R5102 |
T7820 |
T7821 |
compound |
bait,vector |
R5103 |
T7821 |
T7819 |
pobj |
vector,of |
R5104 |
T7822 |
T7821 |
punct |
", ",vector |
R5105 |
T7823 |
T7821 |
conj |
pBUTE,vector |
R5106 |
T7824 |
T7823 |
punct |
(,pBUTE |
R5107 |
T7825 |
T7826 |
det |
a,version |
R5108 |
T7826 |
T7823 |
appos |
version,pBUTE |
R5109 |
T7827 |
T7828 |
npadvmod |
kanamycin,resistant |
R5110 |
T7828 |
T7826 |
amod |
resistant,version |
R5111 |
T7829 |
T7828 |
punct |
-,resistant |
R5112 |
T7830 |
T7826 |
prep |
of,version |
R5113 |
T7831 |
T7832 |
compound |
GAL4,vector |
R5114 |
T7832 |
T7830 |
pobj |
vector,of |
R5115 |
T7833 |
T7832 |
compound |
bait,vector |
R5116 |
T7834 |
T7832 |
appos |
pGBDUC1,vector |
R5117 |
T7835 |
T7823 |
punct |
),pBUTE |
R5118 |
T7836 |
T7837 |
punct |
[,62 |
R5119 |
T7837 |
T7823 |
parataxis |
62,pBUTE |
R5120 |
T7838 |
T7837 |
punct |
],62 |
R5121 |
T7839 |
T7823 |
cc |
and,pBUTE |
R5122 |
T7840 |
T7841 |
compound |
HybriZAP,vector |
R5123 |
T7841 |
T7823 |
conj |
vector,pBUTE |
R5124 |
T7842 |
T7843 |
compound |
pBD,GAL4 |
R5125 |
T7843 |
T7841 |
compound |
GAL4,vector |
R5126 |
T7844 |
T7843 |
punct |
-,GAL4 |
R5127 |
T7845 |
T7846 |
punct |
(,Stratagene |
R5128 |
T7846 |
T7841 |
parataxis |
Stratagene,vector |
R5129 |
T7847 |
T7846 |
punct |
", ",Stratagene |
R5130 |
T7848 |
T7849 |
compound |
La,Jolla |
R5131 |
T7849 |
T7846 |
npadvmod |
Jolla,Stratagene |
R5132 |
T7850 |
T7846 |
punct |
", ",Stratagene |
R5133 |
T7851 |
T7846 |
npadvmod |
California,Stratagene |
R5134 |
T7852 |
T7846 |
punct |
", ",Stratagene |
R5135 |
T7853 |
T7854 |
compound |
United,States |
R5136 |
T7854 |
T7846 |
npadvmod |
States,Stratagene |
R5137 |
T7855 |
T7846 |
punct |
),Stratagene |
R5138 |
T7856 |
T7798 |
punct |
.,subcloned |
R5139 |
T7858 |
T7859 |
det |
The,former |
R5140 |
T7859 |
T7860 |
nsubjpass |
former,used |
R5141 |
T7861 |
T7860 |
auxpass |
was,used |
R5142 |
T7862 |
T7863 |
aux |
to,screen |
R5143 |
T7863 |
T7860 |
advcl |
screen,used |
R5144 |
T7864 |
T7865 |
punct |
~,million |
R5145 |
T7865 |
T7867 |
nummod |
million,clones |
R5146 |
T7866 |
T7865 |
compound |
18,million |
R5147 |
T7867 |
T7863 |
dobj |
clones,screen |
R5148 |
T7868 |
T7867 |
prep |
via,clones |
R5149 |
T7869 |
T7868 |
pobj |
mating,via |
R5150 |
T7870 |
T7867 |
prep |
from,clones |
R5151 |
T7871 |
T7872 |
nmod |
murine,libraries |
R5152 |
T7872 |
T7870 |
pobj |
libraries,from |
R5153 |
T7873 |
T7874 |
quantmod |
9,10 |
R5154 |
T7874 |
T7877 |
nummod |
10,d |
R5155 |
T7875 |
T7874 |
punct |
-,10 |
R5156 |
T7876 |
T7874 |
quantmod |
to,10 |
R5157 |
T7877 |
T7879 |
npadvmod |
d,old |
R5158 |
T7878 |
T7874 |
punct |
-,10 |
R5159 |
T7879 |
T7872 |
amod |
old,libraries |
R5160 |
T7880 |
T7879 |
punct |
-,old |
R5161 |
T7881 |
T7872 |
nmod |
embryo,libraries |
R5162 |
T7882 |
T7881 |
cc |
and,embryo |
R5163 |
T7883 |
T7881 |
conj |
brain,embryo |
R5164 |
T7884 |
T7872 |
compound |
cDNA,libraries |
R5165 |
T7885 |
T7872 |
prep |
at,libraries |
R5166 |
T7886 |
T7887 |
det |
the,Facility |
R5167 |
T7887 |
T7885 |
pobj |
Facility,at |
R5168 |
T7888 |
T7887 |
compound |
Molecular,Facility |
R5169 |
T7889 |
T7887 |
compound |
Interaction,Facility |
R5170 |
T7890 |
T7887 |
punct |
(,Facility |
R5171 |
T7891 |
T7887 |
appos |
MIF,Facility |
R5172 |
T7892 |
T7887 |
punct |
),Facility |
R5173 |
T7893 |
T7887 |
punct |
", ",Facility |
R5174 |
T7894 |
T7887 |
npadvmod |
University,Facility |
R5175 |
T7895 |
T7894 |
prep |
of,University |
R5176 |
T7896 |
T7895 |
pobj |
Wisconsin,of |
R5177 |
T7897 |
T7894 |
punct |
", ",University |
R5178 |
T7898 |
T7894 |
npadvmod |
Madison,University |
R5179 |
T7899 |
T7894 |
punct |
", ",University |
R5180 |
T7900 |
T7894 |
npadvmod |
Wisconsin,University |
R5181 |
T7901 |
T7860 |
punct |
.,used |
R5182 |
T7903 |
T7904 |
det |
The,latter |
R5183 |
T7904 |
T7905 |
nsubjpass |
latter,used |
R5184 |
T7906 |
T7905 |
auxpass |
was,used |
R5185 |
T7907 |
T7908 |
aux |
to,screen |
R5186 |
T7908 |
T7905 |
advcl |
screen,used |
R5187 |
T7909 |
T7908 |
prep |
via,screen |
R5188 |
T7910 |
T7909 |
pobj |
transformation,via |
R5189 |
T7911 |
T7910 |
prep |
of,transformation |
R5190 |
T7912 |
T7913 |
punct |
~,million |
R5191 |
T7913 |
T7915 |
nummod |
million,clones |
R5192 |
T7914 |
T7913 |
compound |
5,million |
R5193 |
T7915 |
T7911 |
pobj |
clones,of |
R5194 |
T7916 |
T7915 |
prep |
from,clones |
R5195 |
T7917 |
T7918 |
amod |
bovine,libraries |
R5196 |
T7918 |
T7916 |
pobj |
libraries,from |
R5197 |
T7919 |
T7918 |
amod |
retinal,libraries |
R5198 |
T7920 |
T7918 |
compound |
cDNA,libraries |
R5199 |
T7921 |
T7922 |
punct |
[,32 |
R5200 |
T7922 |
T7908 |
parataxis |
32,screen |
R5201 |
T7923 |
T7922 |
punct |
],32 |
R5202 |
T7924 |
T7905 |
punct |
.,used |
R5203 |
T7926 |
T7927 |
det |
The,screens |
R5204 |
T7927 |
T7928 |
nsubj |
screens,generated |
R5205 |
T7929 |
T7930 |
nummod |
six,clones |
R5206 |
T7930 |
T7928 |
dobj |
clones,generated |
R5207 |
T7931 |
T7928 |
punct |
.,generated |
R5208 |
T7933 |
T7934 |
nummod |
One,clones |
R5209 |
T7934 |
T7940 |
nsubjpass |
clones,isolated |
R5210 |
T7935 |
T7933 |
cc |
and,One |
R5211 |
T7936 |
T7933 |
conj |
two,One |
R5212 |
T7937 |
T7934 |
nmod |
in,clones |
R5213 |
T7938 |
T7937 |
punct |
-,in |
R5214 |
T7939 |
T7937 |
pobj |
frame,in |
R5215 |
T7941 |
T7940 |
auxpass |
were,isolated |
R5216 |
T7942 |
T7940 |
advmod |
independently,isolated |
R5217 |
T7943 |
T7940 |
prep |
from,isolated |
R5218 |
T7944 |
T7945 |
det |
the,libraries |
R5219 |
T7945 |
T7943 |
pobj |
libraries,from |
R5220 |
T7946 |
T7945 |
amod |
embryonic,libraries |
R5221 |
T7947 |
T7946 |
cc |
and,embryonic |
R5222 |
T7948 |
T7946 |
conj |
adult,embryonic |
R5223 |
T7949 |
T7945 |
compound |
brain,libraries |
R5224 |
T7950 |
T7940 |
punct |
", ",isolated |
R5225 |
T7951 |
T7940 |
advmod |
respectively,isolated |
R5226 |
T7952 |
T7940 |
punct |
", ",isolated |
R5227 |
T7953 |
T7940 |
cc |
and,isolated |
R5228 |
T7954 |
T7955 |
det |
the,interactions |
R5229 |
T7955 |
T7956 |
nsubjpass |
interactions,validated |
R5230 |
T7956 |
T7940 |
conj |
validated,isolated |
R5231 |
T7957 |
T7956 |
auxpass |
were,validated |
R5232 |
T7958 |
T7956 |
punct |
.,validated |
R5233 |
T7960 |
T7961 |
det |
The,clones |
R5234 |
T7961 |
T7963 |
nsubj |
clones,encoded |
R5235 |
T7962 |
T7961 |
nummod |
three,clones |
R5236 |
T7964 |
T7963 |
dobj |
Cox11,encoded |
R5237 |
T7965 |
T7963 |
punct |
.,encoded |
R5238 |
T7967 |
T7968 |
nsubjpass |
Interactions,quantified |
R5239 |
T7969 |
T7967 |
prep |
between,Interactions |
R5240 |
T7970 |
T7969 |
pobj |
Cox11,between |
R5241 |
T7971 |
T7970 |
punct |
", ",Cox11 |
R5242 |
T7972 |
T7970 |
conj |
LD,Cox11 |
R5243 |
T7973 |
T7972 |
punct |
", ",LD |
R5244 |
T7974 |
T7972 |
cc |
and,LD |
R5245 |
T7975 |
T7972 |
conj |
subdomains,LD |
R5246 |
T7976 |
T7975 |
advmod |
thereof,subdomains |
R5247 |
T7977 |
T7968 |
auxpass |
were,quantified |
R5248 |
T7978 |
T7968 |
prep |
by,quantified |
R5249 |
T7979 |
T7980 |
amod |
liquid,galactosidase |
R5250 |
T7980 |
T7978 |
pobj |
galactosidase,by |
R5251 |
T7981 |
T7980 |
compound |
β,galactosidase |
R5252 |
T7982 |
T7980 |
punct |
-,galactosidase |
R5253 |
T7983 |
T7984 |
punct |
(,Biosystems |
R5254 |
T7984 |
T7980 |
parataxis |
Biosystems,galactosidase |
R5255 |
T7985 |
T7984 |
compound |
Applied,Biosystems |
R5256 |
T7986 |
T7984 |
punct |
", ",Biosystems |
R5257 |
T7987 |
T7988 |
compound |
Foster,City |
R5258 |
T7988 |
T7984 |
npadvmod |
City,Biosystems |
R5259 |
T7989 |
T7984 |
punct |
", ",Biosystems |
R5260 |
T7990 |
T7984 |
npadvmod |
California,Biosystems |
R5261 |
T7991 |
T7984 |
punct |
", ",Biosystems |
R5262 |
T7992 |
T7993 |
compound |
United,States |
R5263 |
T7993 |
T7984 |
npadvmod |
States,Biosystems |
R5264 |
T7994 |
T7984 |
punct |
),Biosystems |
R5265 |
T7995 |
T7980 |
cc |
and,galactosidase |
R5266 |
T7996 |
T7997 |
compound |
growth,assays |
R5267 |
T7997 |
T7980 |
conj |
assays,galactosidase |
R5268 |
T7998 |
T7999 |
punct |
[,63 |
R5269 |
T7999 |
T7968 |
parataxis |
63,quantified |
R5270 |
T8000 |
T7999 |
punct |
],63 |
R5271 |
T8001 |
T7968 |
punct |
.,quantified |
R5272 |
T8003 |
T8004 |
det |
The,speed |
R5273 |
T8004 |
T8008 |
nsubjpass |
speed,determined |
R5274 |
T8005 |
T8004 |
nmod |
maximum,speed |
R5275 |
T8006 |
T8004 |
amod |
specific,speed |
R5276 |
T8007 |
T8004 |
compound |
growth,speed |
R5277 |
T8009 |
T8004 |
punct |
(,speed |
R5278 |
T8010 |
T8004 |
appos |
μmax,speed |
R5279 |
T8011 |
T8008 |
punct |
),determined |
R5280 |
T8012 |
T8008 |
auxpass |
was,determined |
R5281 |
T8013 |
T8008 |
prep |
by,determined |
R5282 |
T8014 |
T8013 |
pcomp |
calculating,by |
R5283 |
T8015 |
T8016 |
nsubj |
μmax,x t |
R5284 |
T8016 |
T8014 |
ccomp |
x t,calculating |
R5285 |
T8017 |
T8016 |
punct |
=,x t |
R5286 |
T8018 |
T8016 |
punct |
(,x t |
R5287 |
T8019 |
T8016 |
nmod |
ln,x t |
R5288 |
T8020 |
T8016 |
punct |
(,x t |
R5289 |
T8021 |
T8016 |
punct |
),x t |
R5290 |
T8022 |
T8016 |
punct |
−,x t |
R5291 |
T8023 |
T8024 |
nmod |
ln,x 0 |
R5292 |
T8024 |
T8016 |
appos |
x 0,x t |
R5293 |
T8025 |
T8024 |
punct |
(,x 0 |
R5294 |
T8026 |
T8016 |
punct |
),x t |
R5295 |
T8027 |
T8016 |
punct |
),x t |
R5296 |
T8028 |
T8016 |
punct |
/,x t |
R5297 |
T8029 |
T8016 |
appos |
t,x t |
R5298 |
T8030 |
T8016 |
punct |
", ",x t |
R5299 |
T8031 |
T8032 |
advmod |
where,is |
R5300 |
T8032 |
T8016 |
advcl |
is,x t |
R5301 |
T8033 |
T8032 |
nsubj |
x t,is |
R5302 |
T8034 |
T8035 |
det |
the,OD600 |
R5303 |
T8035 |
T8032 |
attr |
OD600,is |
R5304 |
T8036 |
T8035 |
prep |
of,OD600 |
R5305 |
T8037 |
T8038 |
det |
the,culture |
R5306 |
T8038 |
T8036 |
pobj |
culture,of |
R5307 |
T8039 |
T8035 |
prep |
at,OD600 |
R5308 |
T8040 |
T8041 |
nsubj |
t,t |
R5309 |
T8041 |
T8039 |
pcomp |
t,at |
R5310 |
T8042 |
T8041 |
punct |
=,t |
R5311 |
T8043 |
T8032 |
punct |
", ",is |
R5312 |
T8044 |
T8045 |
nsubj |
x 0,is |
R5313 |
T8045 |
T8032 |
conj |
is,is |
R5314 |
T8046 |
T8047 |
det |
the,OD600 |
R5315 |
T8047 |
T8045 |
attr |
OD600,is |
R5316 |
T8048 |
T8045 |
prep |
at,is |
R5317 |
T8049 |
T8050 |
nsubj |
t,0 |
R5318 |
T8050 |
T8048 |
pcomp |
0,at |
R5319 |
T8051 |
T8050 |
punct |
=,0 |
R5320 |
T8052 |
T8045 |
cc |
and,is |
R5321 |
T8053 |
T8054 |
nsubj |
t,is |
R5322 |
T8054 |
T8045 |
conj |
is,is |
R5323 |
T8055 |
T8056 |
det |
the,time |
R5324 |
T8056 |
T8054 |
attr |
time,is |
R5325 |
T8057 |
T8056 |
prep |
between,time |
R5326 |
T8058 |
T8057 |
pobj |
x 0,between |
R5327 |
T8059 |
T8058 |
cc |
and,x 0 |
R5328 |
T8060 |
T8058 |
conj |
x t,x 0 |
R5329 |
T8061 |
T8008 |
punct |
.,determined |
R5330 |
T8063 |
T8064 |
nsubjpass |
Assays,performed |
R5331 |
T8065 |
T8064 |
auxpass |
were,performed |
R5332 |
T8066 |
T8064 |
prep |
with,performed |
R5333 |
T8067 |
T8068 |
nummod |
three,clones |
R5334 |
T8068 |
T8066 |
pobj |
clones,with |
R5335 |
T8069 |
T8068 |
amod |
independent,clones |
R5336 |
T8070 |
T8068 |
cc |
and,clones |
R5337 |
T8071 |
T8072 |
nummod |
three,samples |
R5338 |
T8072 |
T8068 |
conj |
samples,clones |
R5339 |
T8073 |
T8072 |
prep |
of,samples |
R5340 |
T8074 |
T8075 |
det |
each,clone |
R5341 |
T8075 |
T8073 |
pobj |
clone,of |
R5342 |
T8076 |
T8064 |
punct |
", ",performed |
R5343 |
T8077 |
T8078 |
det |
the,results |
R5344 |
T8078 |
T8079 |
nsubjpass |
results,averaged |
R5345 |
T8079 |
T8064 |
conj |
averaged,performed |
R5346 |
T8080 |
T8079 |
auxpass |
were,averaged |
R5347 |
T8081 |
T8079 |
punct |
", ",averaged |
R5348 |
T8082 |
T8079 |
cc |
and,averaged |
R5349 |
T8083 |
T8084 |
det |
the,deviations |
R5350 |
T8084 |
T8086 |
nsubj |
deviations,calculated |
R5351 |
T8085 |
T8084 |
amod |
standard,deviations |
R5352 |
T8086 |
T8079 |
conj |
calculated,averaged |
R5353 |
T8087 |
T8086 |
punct |
.,calculated |
R5354 |
T8119 |
T8120 |
npadvmod |
Site,directed |
R5355 |
T8120 |
T8122 |
amod |
directed,mutagenesis |
R5356 |
T8121 |
T8120 |
punct |
-,directed |
R5357 |
T8123 |
T8120 |
cc |
and,directed |
R5358 |
T8124 |
T8120 |
conj |
deletion,directed |
R5359 |
T8125 |
T8122 |
cc |
and,mutagenesis |
R5360 |
T8126 |
T8127 |
compound |
plasmid,construction |
R5361 |
T8127 |
T8122 |
conj |
construction,mutagenesis |
R5362 |
T8128 |
T8127 |
punct |
.,construction |
R5363 |
T8130 |
T8131 |
compound |
Deletion,mutagenesis |
R5364 |
T8131 |
T8132 |
nsubjpass |
mutagenesis,carried |
R5365 |
T8133 |
T8132 |
auxpass |
was,carried |
R5366 |
T8134 |
T8132 |
prt |
out,carried |
R5367 |
T8135 |
T8132 |
prep |
with,carried |
R5368 |
T8136 |
T8135 |
pobj |
pairs,with |
R5369 |
T8137 |
T8136 |
prep |
of,pairs |
R5370 |
T8138 |
T8137 |
pobj |
primers,of |
R5371 |
T8139 |
T8138 |
prep |
against,primers |
R5372 |
T8140 |
T8139 |
pobj |
domains,against |
R5373 |
T8141 |
T8140 |
prep |
of,domains |
R5374 |
T8142 |
T8141 |
pobj |
interest,of |
R5375 |
T8143 |
T8140 |
acl |
described,domains |
R5376 |
T8144 |
T8143 |
prep |
in,described |
R5377 |
T8145 |
T8146 |
det |
the,legends |
R5378 |
T8146 |
T8144 |
pobj |
legends,in |
R5379 |
T8147 |
T8146 |
compound |
figure,legends |
R5380 |
T8148 |
T8132 |
punct |
.,carried |
R5381 |
T8150 |
T8151 |
compound |
PCR,products |
R5382 |
T8151 |
T8152 |
nsubjpass |
products,subcloned |
R5383 |
T8153 |
T8152 |
auxpass |
were,subcloned |
R5384 |
T8154 |
T8152 |
prep |
into,subcloned |
R5385 |
T8155 |
T8156 |
compound |
pGEX,KG |
R5386 |
T8156 |
T8154 |
pobj |
KG,into |
R5387 |
T8157 |
T8156 |
punct |
-,KG |
R5388 |
T8158 |
T8159 |
punct |
[,64 |
R5389 |
T8159 |
T8156 |
parataxis |
64,KG |
R5390 |
T8160 |
T8159 |
punct |
],64 |
R5391 |
T8161 |
T8156 |
punct |
", ",KG |
R5392 |
T8162 |
T8163 |
compound |
HybriZAP,vector |
R5393 |
T8163 |
T8156 |
conj |
vector,KG |
R5394 |
T8164 |
T8165 |
compound |
pBD,GAL4 |
R5395 |
T8165 |
T8163 |
compound |
GAL4,vector |
R5396 |
T8166 |
T8165 |
punct |
-,GAL4 |
R5397 |
T8167 |
T8168 |
punct |
(,Stratagene |
R5398 |
T8168 |
T8163 |
parataxis |
Stratagene,vector |
R5399 |
T8169 |
T8168 |
punct |
),Stratagene |
R5400 |
T8170 |
T8163 |
punct |
", ",vector |
R5401 |
T8171 |
T8163 |
cc |
and,vector |
R5402 |
T8172 |
T8173 |
nmod |
pBUTE,vectors |
R5403 |
T8173 |
T8163 |
conj |
vectors,vector |
R5404 |
T8174 |
T8175 |
punct |
[,62 |
R5405 |
T8175 |
T8172 |
parataxis |
62,pBUTE |
R5406 |
T8176 |
T8175 |
punct |
],62 |
R5407 |
T8177 |
T8152 |
punct |
.,subcloned |
R5408 |
T8179 |
T8180 |
compound |
GST,LDZIP |
R5409 |
T8180 |
T8182 |
nsubj |
LDZIP,comprised |
R5410 |
T8181 |
T8180 |
punct |
-,LDZIP |
R5411 |
T8183 |
T8180 |
advmod |
alone,LDZIP |
R5412 |
T8184 |
T8185 |
nmod |
residues,447 |
R5413 |
T8185 |
T8182 |
dobj |
447,comprised |
R5414 |
T8186 |
T8187 |
punct |
–,483 |
R5415 |
T8187 |
T8185 |
prep |
483,447 |
R5416 |
T8188 |
T8185 |
prep |
of,447 |
R5417 |
T8189 |
T8190 |
amod |
human,RanBP2 |
R5418 |
T8190 |
T8188 |
pobj |
RanBP2,of |
R5419 |
T8191 |
T8182 |
punct |
.,comprised |
R5420 |
T8257 |
T8258 |
nmod |
GST,assays |
R5421 |
T8259 |
T8258 |
amod |
pull,assays |
R5422 |
T8260 |
T8259 |
punct |
-,pull |
R5423 |
T8261 |
T8259 |
prt |
down,pull |
R5424 |
T8262 |
T8259 |
cc |
and,pull |
R5425 |
T8263 |
T8259 |
conj |
immunoprecipitation,pull |
R5426 |
T8264 |
T8258 |
punct |
.,assays |
R5427 |
T8266 |
T8267 |
npadvmod |
CHAPS,solubilized |
R5428 |
T8267 |
T8269 |
amod |
solubilized,extracts |
R5429 |
T8268 |
T8267 |
punct |
-,solubilized |
R5430 |
T8269 |
T8271 |
nsubjpass |
extracts,prepared |
R5431 |
T8270 |
T8269 |
amod |
retinal,extracts |
R5432 |
T8272 |
T8269 |
punct |
", ",extracts |
R5433 |
T8273 |
T8269 |
conj |
expression,extracts |
R5434 |
T8274 |
T8273 |
punct |
", ",expression |
R5435 |
T8275 |
T8273 |
cc |
and,expression |
R5436 |
T8276 |
T8273 |
conj |
purification,expression |
R5437 |
T8277 |
T8276 |
prep |
of,purification |
R5438 |
T8278 |
T8279 |
npadvmod |
GST,fused |
R5439 |
T8279 |
T8281 |
amod |
fused,constructs |
R5440 |
T8280 |
T8279 |
punct |
-,fused |
R5441 |
T8281 |
T8277 |
pobj |
constructs,of |
R5442 |
T8282 |
T8271 |
auxpass |
were,prepared |
R5443 |
T8283 |
T8284 |
mark |
as,described |
R5444 |
T8284 |
T8271 |
advcl |
described,prepared |
R5445 |
T8285 |
T8284 |
advmod |
previously,described |
R5446 |
T8286 |
T8287 |
punct |
[,65 |
R5447 |
T8287 |
T8271 |
parataxis |
65,prepared |
R5448 |
T8288 |
T8287 |
punct |
],65 |
R5449 |
T8289 |
T8271 |
punct |
.,prepared |
R5450 |
T8291 |
T8292 |
nmod |
GST,assays |
R5451 |
T8292 |
T8296 |
nsubjpass |
assays,carried |
R5452 |
T8293 |
T8292 |
amod |
pull,assays |
R5453 |
T8294 |
T8293 |
punct |
-,pull |
R5454 |
T8295 |
T8293 |
prt |
down,pull |
R5455 |
T8297 |
T8296 |
auxpass |
were,carried |
R5456 |
T8298 |
T8296 |
prt |
out,carried |
R5457 |
T8299 |
T8296 |
prep |
with,carried |
R5458 |
T8300 |
T8301 |
quantmod |
0.5,2.2 |
R5459 |
T8301 |
T8303 |
nummod |
2.2,μM |
R5460 |
T8302 |
T8301 |
punct |
–,2.2 |
R5461 |
T8303 |
T8299 |
pobj |
μM,with |
R5462 |
T8304 |
T8303 |
prep |
of,μM |
R5463 |
T8305 |
T8306 |
npadvmod |
GST,fused |
R5464 |
T8306 |
T8308 |
amod |
fused,proteins |
R5465 |
T8307 |
T8306 |
punct |
-,fused |
R5466 |
T8308 |
T8304 |
pobj |
proteins,of |
R5467 |
T8309 |
T8310 |
punct |
[,65 |
R5468 |
T8310 |
T8296 |
parataxis |
65,carried |
R5469 |
T8311 |
T8310 |
punct |
],65 |
R5470 |
T8312 |
T8296 |
punct |
.,carried |
R5471 |
T8314 |
T8315 |
nsubjpass |
Co-precipitates,resolved |
R5472 |
T8316 |
T8315 |
auxpass |
were,resolved |
R5473 |
T8317 |
T8315 |
prep |
on,resolved |
R5474 |
T8318 |
T8319 |
compound |
SDS,PAGE |
R5475 |
T8319 |
T8317 |
pobj |
PAGE,on |
R5476 |
T8320 |
T8319 |
punct |
-,PAGE |
R5477 |
T8321 |
T8315 |
cc |
and,resolved |
R5478 |
T8322 |
T8315 |
conj |
analyzed,resolved |
R5479 |
T8323 |
T8322 |
prep |
by,analyzed |
R5480 |
T8324 |
T8325 |
compound |
Western,blot |
R5481 |
T8325 |
T8323 |
pobj |
blot,by |
R5482 |
T8326 |
T8322 |
prep |
with,analyzed |
R5483 |
T8327 |
T8326 |
pobj |
antibodies,with |
R5484 |
T8328 |
T8327 |
acl |
described,antibodies |
R5485 |
T8329 |
T8328 |
prep |
in,described |
R5486 |
T8330 |
T8331 |
det |
the,section |
R5487 |
T8331 |
T8329 |
pobj |
section,in |
R5488 |
T8332 |
T8331 |
compound |
Results,section |
R5489 |
T8333 |
T8315 |
punct |
.,resolved |
R5490 |
T8335 |
T8336 |
amod |
Unfolded,Cox11 |
R5491 |
T8336 |
T8340 |
nsubjpass |
Cox11,generated |
R5492 |
T8337 |
T8335 |
cc |
and,Unfolded |
R5493 |
T8338 |
T8339 |
advmod |
partially,denatured |
R5494 |
T8339 |
T8335 |
conj |
denatured,Unfolded |
R5495 |
T8341 |
T8340 |
auxpass |
were,generated |
R5496 |
T8342 |
T8340 |
prep |
by,generated |
R5497 |
T8343 |
T8342 |
pcomp |
incubating,by |
R5498 |
T8344 |
T8345 |
amod |
recombinant,Cox11 |
R5499 |
T8345 |
T8343 |
dobj |
Cox11,incubating |
R5500 |
T8346 |
T8344 |
cc |
and,recombinant |
R5501 |
T8347 |
T8344 |
conj |
native,recombinant |
R5502 |
T8348 |
T8340 |
advmod |
overnight,generated |
R5503 |
T8349 |
T8340 |
prep |
with,generated |
R5504 |
T8350 |
T8351 |
nummod |
5.6,M |
R5505 |
T8351 |
T8349 |
pobj |
M,with |
R5506 |
T8352 |
T8350 |
cc |
and,5.6 |
R5507 |
T8353 |
T8350 |
conj |
7,5.6 |
R5508 |
T8354 |
T8355 |
compound |
guanidine,hydrochloride |
R5509 |
T8355 |
T8351 |
appos |
hydrochloride,M |
R5510 |
T8356 |
T8355 |
cc |
and,hydrochloride |
R5511 |
T8357 |
T8355 |
conj |
urea,hydrochloride |
R5512 |
T8358 |
T8340 |
punct |
", ",generated |
R5513 |
T8359 |
T8340 |
advmod |
respectively,generated |
R5514 |
T8360 |
T8340 |
punct |
.,generated |
R5515 |
T8362 |
T8363 |
det |
The,conformers |
R5516 |
T8363 |
T8365 |
nsubjpass |
conformers,diluted |
R5517 |
T8364 |
T8363 |
compound |
Cox11,conformers |
R5518 |
T8366 |
T8365 |
auxpass |
were,diluted |
R5519 |
T8367 |
T8365 |
advmod |
then,diluted |
R5520 |
T8368 |
T8369 |
punct |
~,20-fold |
R5521 |
T8369 |
T8365 |
advmod |
20-fold,diluted |
R5522 |
T8370 |
T8365 |
prep |
in,diluted |
R5523 |
T8371 |
T8372 |
compound |
CHAPS,binding |
R5524 |
T8372 |
T8374 |
compound |
binding,buffer |
R5525 |
T8373 |
T8372 |
punct |
-,binding |
R5526 |
T8374 |
T8370 |
pobj |
buffer,in |
R5527 |
T8375 |
T8374 |
acl |
containing,buffer |
R5528 |
T8376 |
T8377 |
compound |
GST,LD |
R5529 |
T8377 |
T8375 |
dobj |
LD,containing |
R5530 |
T8378 |
T8377 |
punct |
-,LD |
R5531 |
T8379 |
T8365 |
punct |
.,diluted |
R5532 |
T8381 |
T8382 |
compound |
Immunoprecipitation,assays |
R5533 |
T8382 |
T8383 |
nsubjpass |
assays,performed |
R5534 |
T8384 |
T8382 |
prep |
with,assays |
R5535 |
T8385 |
T8386 |
nummod |
5,μg |
R5536 |
T8386 |
T8384 |
pobj |
μg,with |
R5537 |
T8387 |
T8386 |
prep |
of,μg |
R5538 |
T8388 |
T8387 |
pobj |
antibody,of |
R5539 |
T8389 |
T8382 |
cc |
and,assays |
R5540 |
T8390 |
T8391 |
compound |
Western,blots |
R5541 |
T8391 |
T8382 |
conj |
blots,assays |
R5542 |
T8392 |
T8393 |
punct |
(,ng |
R5543 |
T8393 |
T8391 |
parataxis |
ng,blots |
R5544 |
T8394 |
T8395 |
punct |
~,400 |
R5545 |
T8395 |
T8393 |
nummod |
400,ng |
R5546 |
T8396 |
T8395 |
quantmod |
200,400 |
R5547 |
T8397 |
T8395 |
punct |
–,400 |
R5548 |
T8398 |
T8399 |
punct |
/,ml |
R5549 |
T8399 |
T8393 |
prep |
ml,ng |
R5550 |
T8400 |
T8399 |
prep |
of,ml |
R5551 |
T8401 |
T8400 |
pobj |
antibody,of |
R5552 |
T8402 |
T8393 |
punct |
),ng |
R5553 |
T8403 |
T8383 |
auxpass |
were,performed |
R5554 |
T8404 |
T8405 |
advmod |
exactly,described |
R5555 |
T8405 |
T8383 |
advcl |
described,performed |
R5556 |
T8406 |
T8405 |
mark |
as,described |
R5557 |
T8407 |
T8405 |
advmod |
previously,described |
R5558 |
T8408 |
T8409 |
punct |
[,13 |
R5559 |
T8409 |
T8405 |
parataxis |
13,described |
R5560 |
T8410 |
T8409 |
punct |
],13 |
R5561 |
T8411 |
T8383 |
punct |
.,performed |
R5562 |
T8483 |
T8484 |
nmod |
Hexokinase,assay |
R5563 |
T8485 |
T8483 |
nummod |
I,Hexokinase |
R5564 |
T8486 |
T8484 |
punct |
.,assay |
R5565 |
T8488 |
T8489 |
nmod |
Hexokinase,activity |
R5566 |
T8489 |
T8491 |
nsubjpass |
activity,determined |
R5567 |
T8490 |
T8488 |
nummod |
I,Hexokinase |
R5568 |
T8492 |
T8491 |
auxpass |
was,determined |
R5569 |
T8493 |
T8491 |
advmod |
spectrophotometrically,determined |
R5570 |
T8494 |
T8491 |
prep |
at,determined |
R5571 |
T8495 |
T8496 |
nummod |
25,°C |
R5572 |
T8496 |
T8494 |
pobj |
°C,at |
R5573 |
T8497 |
T8491 |
prep |
by,determined |
R5574 |
T8498 |
T8499 |
det |
the,method |
R5575 |
T8499 |
T8497 |
pobj |
method,by |
R5576 |
T8500 |
T8499 |
prep |
of,method |
R5577 |
T8501 |
T8500 |
pcomp |
coupling,of |
R5578 |
T8502 |
T8503 |
det |
the,production |
R5579 |
T8503 |
T8501 |
dobj |
production,coupling |
R5580 |
T8504 |
T8505 |
nmod |
glucose,phosphate |
R5581 |
T8505 |
T8503 |
compound |
phosphate,production |
R5582 |
T8506 |
T8505 |
punct |
-,phosphate |
R5583 |
T8507 |
T8505 |
nummod |
6,phosphate |
R5584 |
T8508 |
T8505 |
punct |
-,phosphate |
R5585 |
T8509 |
T8503 |
prep |
via,production |
R5586 |
T8510 |
T8511 |
nmod |
glucose,phosphate |
R5587 |
T8511 |
T8515 |
compound |
phosphate,dehydrogenase |
R5588 |
T8512 |
T8511 |
punct |
-,phosphate |
R5589 |
T8513 |
T8511 |
nummod |
6,phosphate |
R5590 |
T8514 |
T8511 |
punct |
-,phosphate |
R5591 |
T8515 |
T8509 |
pobj |
dehydrogenase,via |
R5592 |
T8516 |
T8501 |
prep |
with,coupling |
R5593 |
T8517 |
T8518 |
det |
the,change |
R5594 |
T8518 |
T8516 |
pobj |
change,with |
R5595 |
T8519 |
T8518 |
prep |
in,change |
R5596 |
T8520 |
T8521 |
det |
the,absorbance |
R5597 |
T8521 |
T8519 |
pobj |
absorbance,in |
R5598 |
T8522 |
T8521 |
prep |
of,absorbance |
R5599 |
T8523 |
T8522 |
pobj |
NADPH,of |
R5600 |
T8524 |
T8518 |
prep |
at,change |
R5601 |
T8525 |
T8526 |
nummod |
340,nm |
R5602 |
T8526 |
T8524 |
pobj |
nm,at |
R5603 |
T8527 |
T8497 |
cc |
and,by |
R5604 |
T8528 |
T8529 |
mark |
as,described |
R5605 |
T8529 |
T8497 |
conj |
described,by |
R5606 |
T8530 |
T8529 |
agent |
by,described |
R5607 |
T8531 |
T8532 |
punct |
[,66 |
R5608 |
T8532 |
T8530 |
pobj |
66,by |
R5609 |
T8533 |
T8491 |
punct |
],determined |
R5610 |
T8534 |
T8491 |
punct |
.,determined |
R5611 |
T8536 |
T8537 |
det |
The,reaction |
R5612 |
T8537 |
T8538 |
nsubjpass |
reaction,started |
R5613 |
T8539 |
T8538 |
auxpass |
was,started |
R5614 |
T8540 |
T8538 |
agent |
by,started |
R5615 |
T8541 |
T8542 |
det |
the,addition |
R5616 |
T8542 |
T8540 |
pobj |
addition,by |
R5617 |
T8543 |
T8542 |
prep |
of,addition |
R5618 |
T8544 |
T8545 |
amod |
purified,hexokinase |
R5619 |
T8545 |
T8543 |
pobj |
hexokinase,of |
R5620 |
T8546 |
T8545 |
compound |
brain,hexokinase |
R5621 |
T8547 |
T8545 |
nummod |
I,hexokinase |
R5622 |
T8548 |
T8549 |
punct |
(,μg |
R5623 |
T8549 |
T8545 |
parataxis |
μg,hexokinase |
R5624 |
T8550 |
T8549 |
nummod |
0.24,μg |
R5625 |
T8551 |
T8549 |
punct |
),μg |
R5626 |
T8552 |
T8553 |
punct |
(,gift |
R5627 |
T8553 |
T8545 |
parataxis |
gift,hexokinase |
R5628 |
T8554 |
T8553 |
prep |
from,gift |
R5629 |
T8555 |
T8556 |
compound |
J.,Wilson |
R5630 |
T8556 |
T8554 |
pobj |
Wilson,from |
R5631 |
T8557 |
T8553 |
punct |
),gift |
R5632 |
T8558 |
T8542 |
prep |
to,addition |
R5633 |
T8559 |
T8560 |
nummod |
1.0,ml |
R5634 |
T8560 |
T8558 |
pobj |
ml,to |
R5635 |
T8561 |
T8560 |
prep |
of,ml |
R5636 |
T8562 |
T8563 |
compound |
reaction,mixture |
R5637 |
T8563 |
T8561 |
pobj |
mixture,of |
R5638 |
T8564 |
T8560 |
acl |
containing,ml |
R5639 |
T8565 |
T8566 |
nummod |
0.05,M |
R5640 |
T8566 |
T8567 |
compound |
M,HCl |
R5641 |
T8567 |
T8564 |
dobj |
HCl,containing |
R5642 |
T8568 |
T8567 |
compound |
Tris,HCl |
R5643 |
T8569 |
T8567 |
punct |
-,HCl |
R5644 |
T8570 |
T8571 |
punct |
(,pH |
R5645 |
T8571 |
T8567 |
parataxis |
pH,HCl |
R5646 |
T8572 |
T8571 |
nummod |
8.5,pH |
R5647 |
T8573 |
T8571 |
punct |
),pH |
R5648 |
T8574 |
T8567 |
punct |
", ",HCl |
R5649 |
T8575 |
T8576 |
nummod |
7.4,mM |
R5650 |
T8576 |
T8577 |
compound |
mM,MgCl2 |
R5651 |
T8577 |
T8567 |
conj |
MgCl2,HCl |
R5652 |
T8578 |
T8577 |
punct |
", ",MgCl2 |
R5653 |
T8579 |
T8580 |
nummod |
6.6,mM |
R5654 |
T8580 |
T8581 |
compound |
mM,ATP |
R5655 |
T8581 |
T8577 |
conj |
ATP,MgCl2 |
R5656 |
T8582 |
T8581 |
punct |
", ",ATP |
R5657 |
T8583 |
T8584 |
nummod |
0.65,mM |
R5658 |
T8584 |
T8585 |
compound |
mM,NADP |
R5659 |
T8585 |
T8581 |
conj |
NADP,ATP |
R5660 |
T8586 |
T8585 |
punct |
", ",NADP |
R5661 |
T8587 |
T8588 |
nummod |
11.1,mM |
R5662 |
T8588 |
T8589 |
compound |
mM,monothioglycerol |
R5663 |
T8589 |
T8585 |
conj |
monothioglycerol,NADP |
R5664 |
T8590 |
T8589 |
punct |
", ",monothioglycerol |
R5665 |
T8591 |
T8589 |
cc |
and,monothioglycerol |
R5666 |
T8592 |
T8593 |
nummod |
1,unit |
R5667 |
T8593 |
T8589 |
conj |
unit,monothioglycerol |
R5668 |
T8594 |
T8593 |
prep |
of,unit |
R5669 |
T8595 |
T8596 |
nmod |
glucose,phosphate |
R5670 |
T8596 |
T8599 |
compound |
phosphate,dehydrogenase |
R5671 |
T8597 |
T8596 |
nummod |
6,phosphate |
R5672 |
T8598 |
T8596 |
punct |
-,phosphate |
R5673 |
T8599 |
T8594 |
pobj |
dehydrogenase,of |
R5674 |
T8600 |
T8538 |
punct |
.,started |
R5675 |
T8602 |
T8603 |
prep |
In,incubated |
R5676 |
T8604 |
T8605 |
det |
the,case |
R5677 |
T8605 |
T8602 |
pobj |
case,In |
R5678 |
T8606 |
T8605 |
prep |
of,case |
R5679 |
T8607 |
T8608 |
compound |
hexokinase,activity |
R5680 |
T8608 |
T8606 |
pobj |
activity,of |
R5681 |
T8609 |
T8608 |
acl |
measured,activity |
R5682 |
T8610 |
T8609 |
prep |
in,measured |
R5683 |
T8611 |
T8612 |
det |
the,presence |
R5684 |
T8612 |
T8610 |
pobj |
presence,in |
R5685 |
T8613 |
T8612 |
prep |
of,presence |
R5686 |
T8614 |
T8613 |
pobj |
Cox11,of |
R5687 |
T8615 |
T8614 |
cc |
and,Cox11 |
R5688 |
T8616 |
T8614 |
conj |
LD,Cox11 |
R5689 |
T8617 |
T8603 |
punct |
", ",incubated |
R5690 |
T8618 |
T8619 |
det |
the,HKI |
R5691 |
T8619 |
T8603 |
nsubjpass |
HKI,incubated |
R5692 |
T8620 |
T8619 |
amod |
purified,HKI |
R5693 |
T8621 |
T8603 |
auxpass |
was,incubated |
R5694 |
T8622 |
T8603 |
prep |
with,incubated |
R5695 |
T8623 |
T8624 |
det |
the,proteins |
R5696 |
T8624 |
T8622 |
pobj |
proteins,with |
R5697 |
T8625 |
T8624 |
amod |
recombinant,proteins |
R5698 |
T8626 |
T8603 |
prep |
for,incubated |
R5699 |
T8627 |
T8628 |
nummod |
15,min |
R5700 |
T8628 |
T8626 |
pobj |
min,for |
R5701 |
T8629 |
T8603 |
prep |
at,incubated |
R5702 |
T8630 |
T8631 |
nummod |
4,°C |
R5703 |
T8631 |
T8629 |
pobj |
°C,at |
R5704 |
T8632 |
T8603 |
prep |
before,incubated |
R5705 |
T8633 |
T8632 |
pobj |
measurement,before |
R5706 |
T8634 |
T8633 |
prep |
of,measurement |
R5707 |
T8635 |
T8636 |
det |
the,activity |
R5708 |
T8636 |
T8634 |
pobj |
activity,of |
R5709 |
T8637 |
T8603 |
punct |
.,incubated |
R5710 |
T8639 |
T8640 |
det |
The,data |
R5711 |
T8640 |
T8641 |
nsubjpass |
data,fitted |
R5712 |
T8642 |
T8641 |
auxpass |
were,fitted |
R5713 |
T8643 |
T8641 |
advmod |
directly,fitted |
R5714 |
T8644 |
T8641 |
prep |
into,fitted |
R5715 |
T8645 |
T8646 |
det |
the,equation |
R5716 |
T8646 |
T8644 |
pobj |
equation,into |
R5717 |
T8647 |
T8648 |
compound |
Michaelis,Menten |
R5718 |
T8648 |
T8646 |
compound |
Menten,equation |
R5719 |
T8649 |
T8648 |
punct |
-,Menten |
R5720 |
T8650 |
T8641 |
advcl |
using,fitted |
R5721 |
T8651 |
T8650 |
dobj |
SIGMAPLOT,using |
R5722 |
T8652 |
T8653 |
punct |
(,Science |
R5723 |
T8653 |
T8651 |
parataxis |
Science,SIGMAPLOT |
R5724 |
T8654 |
T8653 |
compound |
SPSS,Science |
R5725 |
T8655 |
T8653 |
punct |
),Science |
R5726 |
T8656 |
T8641 |
punct |
.,fitted |
R5727 |
T8701 |
T8700 |
punct |
.,Antibodies |
R5728 |
T8703 |
T8704 |
compound |
Rabbit,antisera |
R5729 |
T8704 |
T8705 |
nsubjpass |
antisera,raised |
R5730 |
T8706 |
T8705 |
auxpass |
were,raised |
R5731 |
T8707 |
T8705 |
prep |
against,raised |
R5732 |
T8708 |
T8709 |
det |
the,Cox11 |
R5733 |
T8709 |
T8707 |
pobj |
Cox11,against |
R5734 |
T8710 |
T8709 |
amod |
recombinant,Cox11 |
R5735 |
T8711 |
T8709 |
amod |
murine,Cox11 |
R5736 |
T8712 |
T8713 |
punct |
(,40 |
R5737 |
T8713 |
T8707 |
parataxis |
40,against |
R5738 |
T8714 |
T8713 |
nmod |
residues,40 |
R5739 |
T8715 |
T8716 |
punct |
–,275 |
R5740 |
T8716 |
T8713 |
prep |
275,40 |
R5741 |
T8717 |
T8713 |
punct |
),40 |
R5742 |
T8718 |
T8705 |
cc |
and,raised |
R5743 |
T8719 |
T8720 |
dep |
affinity,purified |
R5744 |
T8720 |
T8705 |
conj |
purified,raised |
R5745 |
T8721 |
T8720 |
punct |
-,purified |
R5746 |
T8722 |
T8720 |
prep |
under,purified |
R5747 |
T8723 |
T8724 |
compound |
non-denaturing,conditions |
R5748 |
T8724 |
T8722 |
pobj |
conditions,under |
R5749 |
T8725 |
T8726 |
punct |
(,Stereogene |
R5750 |
T8726 |
T8724 |
parataxis |
Stereogene,conditions |
R5751 |
T8727 |
T8726 |
punct |
),Stereogene |
R5752 |
T8728 |
T8729 |
mark |
as,described |
R5753 |
T8729 |
T8720 |
advcl |
described,purified |
R5754 |
T8730 |
T8729 |
advmod |
previously,described |
R5755 |
T8731 |
T8732 |
punct |
[,13 |
R5756 |
T8732 |
T8729 |
parataxis |
13,described |
R5757 |
T8733 |
T8732 |
punct |
],13 |
R5758 |
T8734 |
T8705 |
punct |
.,raised |
R5759 |
T8736 |
T8737 |
compound |
Cox11,antibodies |
R5760 |
T8737 |
T8738 |
nsubjpass |
antibodies,used |
R5761 |
T8739 |
T8738 |
auxpass |
were,used |
R5762 |
T8740 |
T8738 |
prep |
at,used |
R5763 |
T8741 |
T8742 |
nummod |
200,ng |
R5764 |
T8742 |
T8740 |
pobj |
ng,at |
R5765 |
T8743 |
T8744 |
punct |
/,ml |
R5766 |
T8744 |
T8742 |
prep |
ml,ng |
R5767 |
T8745 |
T8738 |
prep |
for,used |
R5768 |
T8746 |
T8747 |
compound |
Western,analysis |
R5769 |
T8747 |
T8745 |
pobj |
analysis,for |
R5770 |
T8748 |
T8738 |
punct |
.,used |
R5771 |
T8750 |
T8751 |
det |
The,Hsp70 |
R5772 |
T8751 |
T8754 |
nsubj |
Hsp70,was |
R5773 |
T8752 |
T8751 |
amod |
monoclonal,Hsp70 |
R5774 |
T8753 |
T8751 |
compound |
antibody,Hsp70 |
R5775 |
T8755 |
T8754 |
prep |
from,was |
R5776 |
T8756 |
T8757 |
compound |
Affinity,Bioreagents |
R5777 |
T8757 |
T8755 |
pobj |
Bioreagents,from |
R5778 |
T8758 |
T8759 |
punct |
(,Golden |
R5779 |
T8759 |
T8757 |
parataxis |
Golden,Bioreagents |
R5780 |
T8760 |
T8759 |
punct |
", ",Golden |
R5781 |
T8761 |
T8759 |
npadvmod |
Colorado,Golden |
R5782 |
T8762 |
T8759 |
punct |
", ",Golden |
R5783 |
T8763 |
T8764 |
compound |
United,States |
R5784 |
T8764 |
T8759 |
npadvmod |
States,Golden |
R5785 |
T8765 |
T8759 |
punct |
),Golden |
R5786 |
T8766 |
T8754 |
cc |
and,was |
R5787 |
T8767 |
T8768 |
nsubjpass |
antibodies,described |
R5788 |
T8768 |
T8754 |
conj |
described,was |
R5789 |
T8769 |
T8767 |
prep |
against,antibodies |
R5790 |
T8770 |
T8771 |
det |
the,domains |
R5791 |
T8771 |
T8769 |
pobj |
domains,against |
R5792 |
T8772 |
T8771 |
nmod |
KBD,domains |
R5793 |
T8773 |
T8774 |
punct |
(,JX2 |
R5794 |
T8774 |
T8772 |
parataxis |
JX2,KBD |
R5795 |
T8775 |
T8774 |
punct |
),JX2 |
R5796 |
T8776 |
T8772 |
cc |
and,KBD |
R5797 |
T8777 |
T8772 |
conj |
ZnF,KBD |
R5798 |
T8778 |
T8771 |
prep |
of,domains |
R5799 |
T8779 |
T8778 |
pobj |
RanBP2,of |
R5800 |
T8780 |
T8768 |
aux |
have,described |
R5801 |
T8781 |
T8768 |
auxpass |
been,described |
R5802 |
T8782 |
T8783 |
punct |
[,16 |
R5803 |
T8783 |
T8768 |
parataxis |
16,described |
R5804 |
T8784 |
T8783 |
nummod |
13,16 |
R5805 |
T8785 |
T8783 |
punct |
",",16 |
R5806 |
T8786 |
T8783 |
punct |
],16 |
R5807 |
T8787 |
T8768 |
punct |
.,described |
R5808 |
T8789 |
T8790 |
amod |
Polyclonal,antibodies |
R5809 |
T8790 |
T8793 |
nsubjpass |
antibodies,provided |
R5810 |
T8791 |
T8789 |
cc |
and,Polyclonal |
R5811 |
T8792 |
T8789 |
conj |
monoclonal,Polyclonal |
R5812 |
T8794 |
T8790 |
prep |
against,antibodies |
R5813 |
T8795 |
T8794 |
pobj |
Hexokinase,against |
R5814 |
T8796 |
T8795 |
nmod |
I,Hexokinase |
R5815 |
T8797 |
T8793 |
auxpass |
were,provided |
R5816 |
T8798 |
T8793 |
agent |
by,provided |
R5817 |
T8799 |
T8800 |
compound |
J.,Wilson |
R5818 |
T8800 |
T8798 |
pobj |
Wilson,by |
R5819 |
T8801 |
T8793 |
punct |
.,provided |
R5820 |
T8803 |
T8804 |
det |
The,mAb414 |
R5821 |
T8804 |
T8805 |
nsubjpass |
mAb414,purchased |
R5822 |
T8806 |
T8805 |
auxpass |
was,purchased |
R5823 |
T8807 |
T8805 |
prep |
from,purchased |
R5824 |
T8808 |
T8807 |
pobj |
Abcam,from |
R5825 |
T8809 |
T8810 |
punct |
(,Cambridge |
R5826 |
T8810 |
T8808 |
parataxis |
Cambridge,Abcam |
R5827 |
T8811 |
T8810 |
punct |
", ",Cambridge |
R5828 |
T8812 |
T8810 |
npadvmod |
Massachusetts,Cambridge |
R5829 |
T8813 |
T8810 |
punct |
", ",Cambridge |
R5830 |
T8814 |
T8815 |
compound |
United,States |
R5831 |
T8815 |
T8810 |
npadvmod |
States,Cambridge |
R5832 |
T8816 |
T8810 |
punct |
),Cambridge |
R5833 |
T8817 |
T8805 |
punct |
.,purchased |
R5834 |
T8884 |
T8883 |
cc |
and,Immunocytochemistry |
R5835 |
T8885 |
T8883 |
conj |
microscopy,Immunocytochemistry |
R5836 |
T8886 |
T8885 |
punct |
.,microscopy |
R5837 |
T8888 |
T8889 |
compound |
Retina,dissections |
R5838 |
T8889 |
T8890 |
nsubjpass |
dissections,carried |
R5839 |
T8891 |
T8889 |
punct |
", ",dissections |
R5840 |
T8892 |
T8893 |
amod |
radial,cryosections |
R5841 |
T8893 |
T8889 |
conj |
cryosections,dissections |
R5842 |
T8894 |
T8895 |
punct |
(,μm |
R5843 |
T8895 |
T8893 |
parataxis |
μm,cryosections |
R5844 |
T8896 |
T8897 |
punct |
~,6 |
R5845 |
T8897 |
T8895 |
nummod |
6,μm |
R5846 |
T8898 |
T8895 |
punct |
),μm |
R5847 |
T8899 |
T8893 |
punct |
", ",cryosections |
R5848 |
T8900 |
T8893 |
cc |
and,cryosections |
R5849 |
T8901 |
T8902 |
compound |
immunohistochemistry,procedures |
R5850 |
T8902 |
T8893 |
conj |
procedures,cryosections |
R5851 |
T8903 |
T8890 |
auxpass |
were,carried |
R5852 |
T8904 |
T8890 |
prt |
out,carried |
R5853 |
T8905 |
T8906 |
mark |
as,described |
R5854 |
T8906 |
T8890 |
advcl |
described,carried |
R5855 |
T8907 |
T8906 |
advmod |
elsewhere,described |
R5856 |
T8908 |
T8909 |
punct |
[,19 |
R5857 |
T8909 |
T8906 |
parataxis |
19,described |
R5858 |
T8910 |
T8909 |
punct |
],19 |
R5859 |
T8911 |
T8890 |
punct |
.,carried |
R5860 |
T8913 |
T8914 |
nsubjpass |
Brains,fixed |
R5861 |
T8915 |
T8913 |
prep |
from,Brains |
R5862 |
T8916 |
T8917 |
quantmod |
3,5 |
R5863 |
T8917 |
T8920 |
nummod |
5,mo |
R5864 |
T8918 |
T8917 |
punct |
-,5 |
R5865 |
T8919 |
T8917 |
quantmod |
to,5 |
R5866 |
T8920 |
T8922 |
npadvmod |
mo,old |
R5867 |
T8921 |
T8920 |
punct |
-,mo |
R5868 |
T8922 |
T8924 |
amod |
old,mice |
R5869 |
T8923 |
T8922 |
punct |
-,old |
R5870 |
T8924 |
T8915 |
pobj |
mice,from |
R5871 |
T8925 |
T8924 |
nmod |
C57Bl,mice |
R5872 |
T8926 |
T8925 |
punct |
/,C57Bl |
R5873 |
T8927 |
T8925 |
nummod |
6,C57Bl |
R5874 |
T8928 |
T8914 |
auxpass |
were,fixed |
R5875 |
T8929 |
T8914 |
advmod |
overnight,fixed |
R5876 |
T8930 |
T8914 |
prep |
in,fixed |
R5877 |
T8931 |
T8932 |
nummod |
2,% |
R5878 |
T8932 |
T8933 |
compound |
%,paraformaldehyde |
R5879 |
T8933 |
T8930 |
pobj |
paraformaldehyde,in |
R5880 |
T8934 |
T8914 |
punct |
", ",fixed |
R5881 |
T8935 |
T8914 |
conj |
infused,fixed |
R5882 |
T8936 |
T8935 |
prep |
with,infused |
R5883 |
T8937 |
T8938 |
nummod |
30,% |
R5884 |
T8938 |
T8939 |
compound |
%,sucrose |
R5885 |
T8939 |
T8936 |
pobj |
sucrose,with |
R5886 |
T8940 |
T8935 |
punct |
", ",infused |
R5887 |
T8941 |
T8935 |
cc |
and,infused |
R5888 |
T8942 |
T8935 |
conj |
processed,infused |
R5889 |
T8943 |
T8942 |
prep |
for,processed |
R5890 |
T8944 |
T8943 |
pobj |
cryosectioning,for |
R5891 |
T8945 |
T8914 |
punct |
.,fixed |
R5892 |
T8947 |
T8948 |
amod |
Primary,neurons |
R5893 |
T8948 |
T8950 |
nsubjpass |
neurons,prepared |
R5894 |
T8949 |
T8948 |
compound |
brain,neurons |
R5895 |
T8951 |
T8948 |
cc |
and,neurons |
R5896 |
T8952 |
T8953 |
amod |
glial,cells |
R5897 |
T8953 |
T8948 |
conj |
cells,neurons |
R5898 |
T8954 |
T8950 |
auxpass |
were,prepared |
R5899 |
T8955 |
T8950 |
prep |
from,prepared |
R5900 |
T8956 |
T8957 |
det |
the,cortex |
R5901 |
T8957 |
T8955 |
pobj |
cortex,from |
R5902 |
T8958 |
T8957 |
amod |
cerebral,cortex |
R5903 |
T8959 |
T8950 |
punct |
.,prepared |
R5904 |
T8961 |
T8962 |
nsubjpass |
This,macerated |
R5905 |
T8963 |
T8962 |
auxpass |
was,macerated |
R5906 |
T8964 |
T8962 |
prep |
in,macerated |
R5907 |
T8965 |
T8966 |
det |
the,solution |
R5908 |
T8966 |
T8964 |
pobj |
solution,in |
R5909 |
T8967 |
T8968 |
poss |
Hank,Salt |
R5910 |
T8968 |
T8966 |
compound |
Salt,solution |
R5911 |
T8969 |
T8967 |
case |
's,Hank |
R5912 |
T8970 |
T8968 |
compound |
Balanced,Salt |
R5913 |
T8971 |
T8962 |
cc |
and,macerated |
R5914 |
T8972 |
T8962 |
conj |
triturated,macerated |
R5915 |
T8973 |
T8962 |
punct |
.,macerated |
R5916 |
T8975 |
T8976 |
amod |
Primary,cells |
R5917 |
T8976 |
T8977 |
nsubjpass |
cells,cultured |
R5918 |
T8978 |
T8977 |
auxpass |
were,cultured |
R5919 |
T8979 |
T8977 |
prep |
in,cultured |
R5920 |
T8980 |
T8979 |
pobj |
DMEM,in |
R5921 |
T8981 |
T8982 |
punct |
(,GIBCO |
R5922 |
T8982 |
T8980 |
parataxis |
GIBCO,DMEM |
R5923 |
T8983 |
T8982 |
punct |
", ",GIBCO |
R5924 |
T8984 |
T8985 |
compound |
San,Diego |
R5925 |
T8985 |
T8982 |
npadvmod |
Diego,GIBCO |
R5926 |
T8986 |
T8982 |
punct |
", ",GIBCO |
R5927 |
T8987 |
T8982 |
npadvmod |
California,GIBCO |
R5928 |
T8988 |
T8982 |
punct |
", ",GIBCO |
R5929 |
T8989 |
T8990 |
compound |
United,States |
R5930 |
T8990 |
T8982 |
npadvmod |
States,GIBCO |
R5931 |
T8991 |
T8982 |
punct |
),GIBCO |
R5932 |
T8992 |
T8980 |
cc |
and,DMEM |
R5933 |
T8993 |
T8994 |
npadvmod |
collagen,coated |
R5934 |
T8994 |
T8996 |
amod |
coated,dishes |
R5935 |
T8995 |
T8994 |
punct |
-,coated |
R5936 |
T8996 |
T8980 |
conj |
dishes,DMEM |
R5937 |
T8997 |
T8998 |
nummod |
35,mm |
R5938 |
T8998 |
T8996 |
compound |
mm,dishes |
R5939 |
T8999 |
T8998 |
punct |
-,mm |
R5940 |
T9000 |
T9001 |
compound |
glass,bottom |
R5941 |
T9001 |
T8996 |
compound |
bottom,dishes |
R5942 |
T9002 |
T8996 |
compound |
culture,dishes |
R5943 |
T9003 |
T9004 |
punct |
(,Corporation |
R5944 |
T9004 |
T8996 |
parataxis |
Corporation,dishes |
R5945 |
T9005 |
T9004 |
compound |
MatTek,Corporation |
R5946 |
T9006 |
T9004 |
punct |
", ",Corporation |
R5947 |
T9007 |
T9004 |
npadvmod |
Ashland,Corporation |
R5948 |
T9008 |
T9004 |
punct |
", ",Corporation |
R5949 |
T9009 |
T9004 |
npadvmod |
Maine,Corporation |
R5950 |
T9010 |
T9004 |
punct |
),Corporation |
R5951 |
T9011 |
T8977 |
prep |
at,cultured |
R5952 |
T9012 |
T9013 |
nummod |
5,% |
R5953 |
T9013 |
T9014 |
compound |
%,CO2 |
R5954 |
T9014 |
T9011 |
pobj |
CO2,at |
R5955 |
T9015 |
T9014 |
punct |
/,CO2 |
R5956 |
T9016 |
T9017 |
nummod |
37,°C |
R5957 |
T9017 |
T9014 |
appos |
°C,CO2 |
R5958 |
T9018 |
T8977 |
prep |
for,cultured |
R5959 |
T9019 |
T9020 |
punct |
~,2 |
R5960 |
T9020 |
T9021 |
nummod |
2,d |
R5961 |
T9021 |
T9018 |
pobj |
d,for |
R5962 |
T9022 |
T8977 |
cc |
and,cultured |
R5963 |
T9023 |
T8977 |
conj |
processed,cultured |
R5964 |
T9024 |
T9023 |
prep |
for,processed |
R5965 |
T9025 |
T9024 |
pobj |
immunocytochemistry,for |
R5966 |
T9026 |
T8977 |
punct |
.,cultured |
R5967 |
T9028 |
T9029 |
amod |
Primary,antibodies |
R5968 |
T9029 |
T9030 |
nsubjpass |
antibodies,used |
R5969 |
T9031 |
T9030 |
auxpass |
were,used |
R5970 |
T9032 |
T9030 |
prep |
at,used |
R5971 |
T9033 |
T9032 |
pobj |
concentrations,at |
R5972 |
T9034 |
T9035 |
punct |
~,10 |
R5973 |
T9035 |
T9038 |
nummod |
10,μg |
R5974 |
T9036 |
T9035 |
quantmod |
2.5,10 |
R5975 |
T9037 |
T9035 |
punct |
–,10 |
R5976 |
T9038 |
T9033 |
npadvmod |
μg,concentrations |
R5977 |
T9039 |
T9040 |
punct |
/,ml |
R5978 |
T9040 |
T9038 |
prep |
ml,μg |
R5979 |
T9041 |
T9030 |
punct |
.,used |
R5980 |
T9043 |
T9044 |
npadvmod |
Alexa,conjugated |
R5981 |
T9044 |
T9051 |
amod |
conjugated,antibodies |
R5982 |
T9045 |
T9043 |
nummod |
488,Alexa |
R5983 |
T9046 |
T9043 |
punct |
-,Alexa |
R5984 |
T9047 |
T9043 |
cc |
and,Alexa |
R5985 |
T9048 |
T9043 |
conj |
Alexa,Alexa |
R5986 |
T9049 |
T9048 |
amod |
594,Alexa |
R5987 |
T9050 |
T9044 |
punct |
-,conjugated |
R5988 |
T9051 |
T9053 |
nsubjpass |
antibodies,used |
R5989 |
T9052 |
T9051 |
amod |
secondary,antibodies |
R5990 |
T9054 |
T9055 |
punct |
(,μg |
R5991 |
T9055 |
T9051 |
parataxis |
μg,antibodies |
R5992 |
T9056 |
T9055 |
nummod |
2.5,μg |
R5993 |
T9057 |
T9058 |
punct |
/,ml |
R5994 |
T9058 |
T9055 |
prep |
ml,μg |
R5995 |
T9059 |
T9055 |
punct |
),μg |
R5996 |
T9060 |
T9061 |
punct |
(,Probes |
R5997 |
T9061 |
T9051 |
parataxis |
Probes,antibodies |
R5998 |
T9062 |
T9061 |
compound |
Molecular,Probes |
R5999 |
T9063 |
T9061 |
punct |
),Probes |
R6000 |
T9064 |
T9053 |
auxpass |
were,used |
R6001 |
T9065 |
T9053 |
prep |
for,used |
R6002 |
T9066 |
T9065 |
pobj |
visualization,for |
R6003 |
T9067 |
T9066 |
prep |
of,visualization |
R6004 |
T9068 |
T9067 |
pobj |
proteins,of |
R6005 |
T9069 |
T9053 |
punct |
.,used |
R6006 |
T9071 |
T9072 |
nsubjpass |
Crossover,found |
R6007 |
T9073 |
T9071 |
prep |
of,Crossover |
R6008 |
T9074 |
T9075 |
amod |
fluorescent,probes |
R6009 |
T9075 |
T9073 |
pobj |
probes,of |
R6010 |
T9076 |
T9075 |
punct |
", ",probes |
R6011 |
T9077 |
T9075 |
conj |
background,probes |
R6012 |
T9078 |
T9077 |
punct |
", ",background |
R6013 |
T9079 |
T9077 |
cc |
and,background |
R6014 |
T9080 |
T9077 |
conj |
autofluorescence,background |
R6015 |
T9081 |
T9072 |
auxpass |
were,found |
R6016 |
T9082 |
T9083 |
aux |
to,be |
R6017 |
T9083 |
T9072 |
xcomp |
be,found |
R6018 |
T9084 |
T9083 |
acomp |
negligible,be |
R6019 |
T9085 |
T9072 |
punct |
.,found |
R6020 |
T9087 |
T9088 |
nsubjpass |
Visualization,carried |
R6021 |
T9089 |
T9087 |
prep |
of,Visualization |
R6022 |
T9090 |
T9089 |
pobj |
specimens,of |
R6023 |
T9091 |
T9087 |
cc |
and,Visualization |
R6024 |
T9092 |
T9087 |
conj |
localization,Visualization |
R6025 |
T9093 |
T9092 |
prep |
of,localization |
R6026 |
T9094 |
T9093 |
pobj |
proteins,of |
R6027 |
T9095 |
T9088 |
auxpass |
were,carried |
R6028 |
T9096 |
T9088 |
prt |
out,carried |
R6029 |
T9097 |
T9088 |
prep |
by,carried |
R6030 |
T9098 |
T9099 |
amod |
wide,field |
R6031 |
T9099 |
T9101 |
compound |
field,microscopy |
R6032 |
T9100 |
T9099 |
punct |
-,field |
R6033 |
T9101 |
T9097 |
pobj |
microscopy,by |
R6034 |
T9102 |
T9101 |
compound |
epifluorescence,microscopy |
R6035 |
T9103 |
T9088 |
prep |
on,carried |
R6036 |
T9104 |
T9105 |
nmod |
Nikon,upright |
R6037 |
T9105 |
T9103 |
pobj |
upright,on |
R6038 |
T9106 |
T9107 |
punct |
(,Tokyo |
R6039 |
T9107 |
T9104 |
parataxis |
Tokyo,Nikon |
R6040 |
T9108 |
T9107 |
punct |
", ",Tokyo |
R6041 |
T9109 |
T9107 |
npadvmod |
Japan,Tokyo |
R6042 |
T9110 |
T9107 |
punct |
),Tokyo |
R6043 |
T9111 |
T9105 |
compound |
E600,upright |
R6044 |
T9112 |
T9105 |
cc |
and,upright |
R6045 |
T9113 |
T9114 |
nmod |
TE2000U,microscopes |
R6046 |
T9114 |
T9105 |
conj |
microscopes,upright |
R6047 |
T9115 |
T9113 |
amod |
inverted,TE2000U |
R6048 |
T9116 |
T9114 |
compound |
research,microscopes |
R6049 |
T9117 |
T9105 |
acl |
equipped,upright |
R6050 |
T9118 |
T9117 |
prep |
with,equipped |
R6051 |
T9119 |
T9120 |
amod |
similar,objectives |
R6052 |
T9120 |
T9118 |
pobj |
objectives,with |
R6053 |
T9121 |
T9120 |
compound |
Apochromat,objectives |
R6054 |
T9122 |
T9088 |
punct |
.,carried |
R6055 |
T9124 |
T9125 |
nsubjpass |
Images,acquired |
R6056 |
T9126 |
T9124 |
prep |
with,Images |
R6057 |
T9127 |
T9128 |
det |
the,E600 |
R6058 |
T9128 |
T9126 |
pobj |
E600,with |
R6059 |
T9129 |
T9128 |
compound |
Nikon,E600 |
R6060 |
T9130 |
T9125 |
auxpass |
were,acquired |
R6061 |
T9131 |
T9125 |
prep |
with,acquired |
R6062 |
T9132 |
T9133 |
det |
a,camera |
R6063 |
T9133 |
T9131 |
pobj |
camera,with |
R6064 |
T9134 |
T9135 |
nmod |
SPOT,RT |
R6065 |
T9135 |
T9133 |
nmod |
RT,camera |
R6066 |
T9136 |
T9135 |
punct |
-,RT |
R6067 |
T9137 |
T9133 |
amod |
digital,camera |
R6068 |
T9138 |
T9133 |
acl |
coupled,camera |
R6069 |
T9139 |
T9138 |
prep |
to,coupled |
R6070 |
T9140 |
T9141 |
det |
the,microscope |
R6071 |
T9141 |
T9139 |
pobj |
microscope,to |
R6072 |
T9142 |
T9138 |
cc |
and,coupled |
R6073 |
T9143 |
T9138 |
conj |
driven,coupled |
R6074 |
T9144 |
T9143 |
agent |
by,driven |
R6075 |
T9145 |
T9146 |
compound |
SPOT,v4.0 |
R6076 |
T9146 |
T9148 |
compound |
v4.0,software |
R6077 |
T9147 |
T9146 |
compound |
Imaging,v4.0 |
R6078 |
T9148 |
T9144 |
pobj |
software,by |
R6079 |
T9149 |
T9150 |
punct |
(,Instruments |
R6080 |
T9150 |
T9148 |
parataxis |
Instruments,software |
R6081 |
T9151 |
T9150 |
compound |
Diagnostic,Instruments |
R6082 |
T9152 |
T9150 |
punct |
", ",Instruments |
R6083 |
T9153 |
T9154 |
compound |
Sterling,Heights |
R6084 |
T9154 |
T9150 |
npadvmod |
Heights,Instruments |
R6085 |
T9155 |
T9150 |
punct |
", ",Instruments |
R6086 |
T9156 |
T9150 |
npadvmod |
Michigan,Instruments |
R6087 |
T9157 |
T9150 |
punct |
", ",Instruments |
R6088 |
T9158 |
T9159 |
compound |
United,States |
R6089 |
T9159 |
T9150 |
npadvmod |
States,Instruments |
R6090 |
T9160 |
T9150 |
punct |
),Instruments |
R6091 |
T9161 |
T9125 |
punct |
.,acquired |
R6092 |
T9163 |
T9164 |
det |
All,images |
R6093 |
T9164 |
T9165 |
nsubjpass |
images,captured |
R6094 |
T9166 |
T9165 |
auxpass |
were,captured |
R6095 |
T9167 |
T9165 |
prep |
at,captured |
R6096 |
T9168 |
T9169 |
compound |
nonsaturating,levels |
R6097 |
T9169 |
T9167 |
pobj |
levels,at |
R6098 |
T9170 |
T9169 |
compound |
integration,levels |
R6099 |
T9171 |
T9169 |
punct |
", ",levels |
R6100 |
T9172 |
T9173 |
nummod |
12,bit |
R6101 |
T9173 |
T9175 |
nmod |
bit,white |
R6102 |
T9174 |
T9173 |
punct |
-,bit |
R6103 |
T9175 |
T9169 |
appos |
white,levels |
R6104 |
T9176 |
T9175 |
nmod |
mono,white |
R6105 |
T9177 |
T9175 |
amod |
black,white |
R6106 |
T9178 |
T9175 |
punct |
/,white |
R6107 |
T9179 |
T9165 |
punct |
", ",captured |
R6108 |
T9180 |
T9165 |
cc |
and,captured |
R6109 |
T9181 |
T9182 |
advmod |
then,pseudo-colored |
R6110 |
T9182 |
T9165 |
conj |
pseudo-colored,captured |
R6111 |
T9183 |
T9165 |
punct |
.,captured |
R6112 |
T9185 |
T9186 |
compound |
Protein,colocalization |
R6113 |
T9186 |
T9187 |
compound |
colocalization,analysis |
R6114 |
T9187 |
T9188 |
nsubjpass |
analysis,carried |
R6115 |
T9189 |
T9188 |
auxpass |
was,carried |
R6116 |
T9190 |
T9188 |
prt |
out,carried |
R6117 |
T9191 |
T9188 |
prep |
on,carried |
R6118 |
T9192 |
T9193 |
det |
the,microscope |
R6119 |
T9193 |
T9191 |
pobj |
microscope,on |
R6120 |
T9194 |
T9195 |
compound |
Nikon,TE2000U |
R6121 |
T9195 |
T9193 |
compound |
TE2000U,microscope |
R6122 |
T9196 |
T9193 |
acl |
equipped,microscope |
R6123 |
T9197 |
T9196 |
prep |
with,equipped |
R6124 |
T9198 |
T9199 |
amod |
appropriate,wheels |
R6125 |
T9199 |
T9197 |
pobj |
wheels,with |
R6126 |
T9200 |
T9199 |
nmod |
excitation,wheels |
R6127 |
T9201 |
T9200 |
cc |
and,excitation |
R6128 |
T9202 |
T9200 |
conj |
emission,excitation |
R6129 |
T9203 |
T9199 |
compound |
filter,wheels |
R6130 |
T9204 |
T9199 |
punct |
", ",wheels |
R6131 |
T9205 |
T9206 |
compound |
cube,filters |
R6132 |
T9206 |
T9199 |
appos |
filters,wheels |
R6133 |
T9207 |
T9199 |
punct |
", ",wheels |
R6134 |
T9208 |
T9209 |
nummod |
100,W |
R6135 |
T9209 |
T9210 |
compound |
W,source |
R6136 |
T9210 |
T9199 |
appos |
source,wheels |
R6137 |
T9211 |
T9210 |
compound |
mercury,source |
R6138 |
T9212 |
T9210 |
compound |
light,source |
R6139 |
T9213 |
T9199 |
punct |
", ",wheels |
R6140 |
T9214 |
T9215 |
nmod |
Nomarski,DIC |
R6141 |
T9215 |
T9217 |
nmod |
DIC,optics |
R6142 |
T9216 |
T9215 |
punct |
/,DIC |
R6143 |
T9217 |
T9199 |
appos |
optics,wheels |
R6144 |
T9218 |
T9215 |
punct |
", ",DIC |
R6145 |
T9219 |
T9215 |
cc |
and,DIC |
R6146 |
T9220 |
T9221 |
compound |
Plan,Apochromat |
R6147 |
T9221 |
T9215 |
conj |
Apochromat,DIC |
R6148 |
T9222 |
T9217 |
punct |
(,optics |
R6149 |
T9223 |
T9224 |
nummod |
100,objectives |
R6150 |
T9224 |
T9217 |
appos |
objectives,optics |
R6151 |
T9225 |
T9223 |
punct |
×,100 |
R6152 |
T9226 |
T9223 |
punct |
", ",100 |
R6153 |
T9227 |
T9223 |
conj |
60,100 |
R6154 |
T9228 |
T9227 |
punct |
×,60 |
R6155 |
T9229 |
T9227 |
punct |
", ",60 |
R6156 |
T9230 |
T9227 |
cc |
and,60 |
R6157 |
T9231 |
T9227 |
conj |
40,60 |
R6158 |
T9232 |
T9231 |
punct |
×,40 |
R6159 |
T9233 |
T9224 |
compound |
oil,objectives |
R6160 |
T9234 |
T9224 |
prep |
with,objectives |
R6161 |
T9235 |
T9234 |
pobj |
NA,with |
R6162 |
T9236 |
T9235 |
prep |
of,NA |
R6163 |
T9237 |
T9236 |
pobj |
1.4,of |
R6164 |
T9238 |
T9224 |
punct |
", ",objectives |
R6165 |
T9239 |
T9240 |
nummod |
40,LWD |
R6166 |
T9240 |
T9242 |
nmod |
LWD,objectives |
R6167 |
T9241 |
T9239 |
punct |
×,40 |
R6168 |
T9242 |
T9224 |
conj |
objectives,objectives |
R6169 |
T9243 |
T9240 |
punct |
", ",LWD |
R6170 |
T9244 |
T9240 |
cc |
and,LWD |
R6171 |
T9245 |
T9246 |
nummod |
20,LWD |
R6172 |
T9246 |
T9240 |
conj |
LWD,LWD |
R6173 |
T9247 |
T9245 |
punct |
×,20 |
R6174 |
T9248 |
T9242 |
cc |
and,objectives |
R6175 |
T9249 |
T9250 |
amod |
encoded,Stage |
R6176 |
T9250 |
T9242 |
conj |
Stage,objectives |
R6177 |
T9251 |
T9250 |
amod |
motorized,Stage |
R6178 |
T9252 |
T9250 |
compound |
Z,Stage |
R6179 |
T9253 |
T9250 |
punct |
-,Stage |
R6180 |
T9254 |
T9255 |
punct |
(,Scientific |
R6181 |
T9255 |
T9250 |
parataxis |
Scientific,Stage |
R6182 |
T9256 |
T9255 |
compound |
Prior,Scientific |
R6183 |
T9257 |
T9255 |
punct |
", ",Scientific |
R6184 |
T9258 |
T9255 |
npadvmod |
Rockland,Scientific |
R6185 |
T9259 |
T9255 |
punct |
", ",Scientific |
R6186 |
T9260 |
T9255 |
npadvmod |
Maryland,Scientific |
R6187 |
T9261 |
T9255 |
punct |
", ",Scientific |
R6188 |
T9262 |
T9263 |
compound |
United,States |
R6189 |
T9263 |
T9255 |
npadvmod |
States,Scientific |
R6190 |
T9264 |
T9255 |
punct |
),Scientific |
R6191 |
T9265 |
T9188 |
punct |
.,carried |
R6192 |
T9267 |
T9268 |
nsubjpass |
Images,obtained |
R6193 |
T9269 |
T9267 |
prep |
with,Images |
R6194 |
T9270 |
T9271 |
det |
the,microscope |
R6195 |
T9271 |
T9269 |
pobj |
microscope,with |
R6196 |
T9272 |
T9271 |
amod |
inverted,microscope |
R6197 |
T9273 |
T9274 |
compound |
Nikon,TE2000U |
R6198 |
T9274 |
T9271 |
compound |
TE2000U,microscope |
R6199 |
T9275 |
T9268 |
auxpass |
were,obtained |
R6200 |
T9276 |
T9268 |
advcl |
using,obtained |
R6201 |
T9277 |
T9278 |
det |
a,camera |
R6202 |
T9278 |
T9276 |
dobj |
camera,using |
R6203 |
T9279 |
T9278 |
compound |
CCD,camera |
R6204 |
T9280 |
T9278 |
punct |
(,camera |
R6205 |
T9281 |
T9282 |
compound |
CoolSNAP,HQ |
R6206 |
T9282 |
T9278 |
appos |
HQ,camera |
R6207 |
T9283 |
T9284 |
punct |
;,Scientific |
R6208 |
T9284 |
T9278 |
parataxis |
Scientific,camera |
R6209 |
T9285 |
T9284 |
compound |
Roper,Scientific |
R6210 |
T9286 |
T9284 |
punct |
", ",Scientific |
R6211 |
T9287 |
T9284 |
npadvmod |
Trenton,Scientific |
R6212 |
T9288 |
T9284 |
punct |
", ",Scientific |
R6213 |
T9289 |
T9290 |
compound |
New,Jersey |
R6214 |
T9290 |
T9284 |
npadvmod |
Jersey,Scientific |
R6215 |
T9291 |
T9284 |
punct |
", ",Scientific |
R6216 |
T9292 |
T9293 |
compound |
United,States |
R6217 |
T9293 |
T9284 |
npadvmod |
States,Scientific |
R6218 |
T9294 |
T9284 |
punct |
),Scientific |
R6219 |
T9295 |
T9268 |
punct |
.,obtained |
R6220 |
T9297 |
T9298 |
nsubjpass |
Images,analyzed |
R6221 |
T9299 |
T9297 |
acl |
acquired,Images |
R6222 |
T9300 |
T9299 |
prep |
under,acquired |
R6223 |
T9301 |
T9302 |
amod |
identical,parameters |
R6224 |
T9302 |
T9300 |
pobj |
parameters,under |
R6225 |
T9303 |
T9302 |
compound |
acquisition,parameters |
R6226 |
T9304 |
T9298 |
auxpass |
were,analyzed |
R6227 |
T9305 |
T9298 |
advcl |
using,analyzed |
R6228 |
T9306 |
T9307 |
compound |
Metamorph,v6.2 |
R6229 |
T9307 |
T9305 |
dobj |
v6.2,using |
R6230 |
T9308 |
T9307 |
compound |
Software,v6.2 |
R6231 |
T9309 |
T9310 |
punct |
(,Imaging |
R6232 |
T9310 |
T9307 |
parataxis |
Imaging,v6.2 |
R6233 |
T9311 |
T9310 |
compound |
Universal,Imaging |
R6234 |
T9312 |
T9310 |
punct |
", ",Imaging |
R6235 |
T9313 |
T9310 |
npadvmod |
Glendale,Imaging |
R6236 |
T9314 |
T9310 |
punct |
", ",Imaging |
R6237 |
T9315 |
T9310 |
npadvmod |
Wisconsin,Imaging |
R6238 |
T9316 |
T9310 |
punct |
", ",Imaging |
R6239 |
T9317 |
T9318 |
compound |
United,States |
R6240 |
T9318 |
T9310 |
npadvmod |
States,Imaging |
R6241 |
T9319 |
T9310 |
punct |
),Imaging |
R6242 |
T9320 |
T9298 |
punct |
.,analyzed |
R6243 |
T9322 |
T9323 |
advmod |
Whenever,applicable |
R6244 |
T9323 |
T9324 |
advcl |
applicable,captured |
R6245 |
T9325 |
T9324 |
punct |
", ",captured |
R6246 |
T9326 |
T9327 |
amod |
serial,stacks |
R6247 |
T9327 |
T9324 |
nsubjpass |
stacks,captured |
R6248 |
T9328 |
T9327 |
amod |
optical,stacks |
R6249 |
T9329 |
T9327 |
compound |
Z,stacks |
R6250 |
T9330 |
T9327 |
punct |
-,stacks |
R6251 |
T9331 |
T9327 |
punct |
(,stacks |
R6252 |
T9332 |
T9333 |
quantmod |
20,30 |
R6253 |
T9333 |
T9335 |
nummod |
30,planes |
R6254 |
T9334 |
T9333 |
punct |
–,30 |
R6255 |
T9335 |
T9327 |
appos |
planes,stacks |
R6256 |
T9336 |
T9335 |
amod |
focal,planes |
R6257 |
T9337 |
T9335 |
prep |
at,planes |
R6258 |
T9338 |
T9339 |
nummod |
100,nm |
R6259 |
T9339 |
T9341 |
compound |
nm,intervals |
R6260 |
T9340 |
T9339 |
punct |
-,nm |
R6261 |
T9341 |
T9337 |
pobj |
intervals,at |
R6262 |
T9342 |
T9324 |
punct |
),captured |
R6263 |
T9343 |
T9324 |
auxpass |
were,captured |
R6264 |
T9344 |
T9324 |
cc |
and,captured |
R6265 |
T9345 |
T9346 |
advmod |
computationally,processed |
R6266 |
T9346 |
T9324 |
conj |
processed,captured |
R6267 |
T9347 |
T9346 |
prep |
by,processed |
R6268 |
T9348 |
T9349 |
nummod |
3,D |
R6269 |
T9349 |
T9351 |
nmod |
D,methods |
R6270 |
T9350 |
T9349 |
punct |
-,D |
R6271 |
T9351 |
T9347 |
pobj |
methods,by |
R6272 |
T9352 |
T9351 |
amod |
blind,methods |
R6273 |
T9353 |
T9351 |
compound |
deconvolution,methods |
R6274 |
T9354 |
T9346 |
prep |
with,processed |
R6275 |
T9355 |
T9356 |
det |
the,software |
R6276 |
T9356 |
T9354 |
pobj |
software,with |
R6277 |
T9357 |
T9356 |
amod |
same,software |
R6278 |
T9358 |
T9324 |
punct |
.,captured |
R6279 |
T9492 |
T9491 |
prep |
of,Generation |
R6280 |
T9493 |
T9494 |
det |
a,line |
R6281 |
T9494 |
T9492 |
pobj |
line,of |
R6282 |
T9495 |
T9494 |
compound |
mouse,line |
R6283 |
T9496 |
T9494 |
prep |
with,line |
R6284 |
T9497 |
T9498 |
det |
a,locus |
R6285 |
T9498 |
T9496 |
pobj |
locus,with |
R6286 |
T9499 |
T9498 |
amod |
disrupted,locus |
R6287 |
T9500 |
T9498 |
compound |
RanBP2,locus |
R6288 |
T9501 |
T9491 |
cc |
and,Generation |
R6289 |
T9502 |
T9503 |
amod |
histological,analysis |
R6290 |
T9503 |
T9491 |
conj |
analysis,Generation |
R6291 |
T9504 |
T9503 |
prep |
of,analysis |
R6292 |
T9505 |
T9506 |
amod |
trapped,mice |
R6293 |
T9506 |
T9504 |
pobj |
mice,of |
R6294 |
T9507 |
T9506 |
compound |
RanBP2,mice |
R6295 |
T9508 |
T9503 |
punct |
.,analysis |
R6296 |
T9510 |
T9511 |
det |
A,line |
R6297 |
T9511 |
T9518 |
nsubjpass |
line,obtained |
R6298 |
T9512 |
T9513 |
npadvmod |
feeder,independent |
R6299 |
T9513 |
T9511 |
amod |
independent,line |
R6300 |
T9514 |
T9513 |
punct |
-,independent |
R6301 |
T9515 |
T9511 |
compound |
mouse,line |
R6302 |
T9516 |
T9517 |
compound |
ES,cell |
R6303 |
T9517 |
T9511 |
compound |
cell,line |
R6304 |
T9519 |
T9511 |
acl |
derived,line |
R6305 |
T9520 |
T9519 |
prep |
from,derived |
R6306 |
T9521 |
T9522 |
det |
the,strain |
R6307 |
T9522 |
T9520 |
pobj |
strain,from |
R6308 |
T9523 |
T9524 |
compound |
129P2,OlaHsd |
R6309 |
T9524 |
T9522 |
compound |
OlaHsd,strain |
R6310 |
T9525 |
T9524 |
punct |
/,OlaHsd |
R6311 |
T9526 |
T9519 |
cc |
and,derived |
R6312 |
T9527 |
T9519 |
conj |
harboring,derived |
R6313 |
T9528 |
T9529 |
det |
a,locus |
R6314 |
T9529 |
T9527 |
dobj |
locus,harboring |
R6315 |
T9530 |
T9529 |
amod |
disrupted,locus |
R6316 |
T9531 |
T9529 |
compound |
RanBP2,locus |
R6317 |
T9532 |
T9529 |
prep |
by,locus |
R6318 |
T9533 |
T9534 |
compound |
insertion,mutagenesis |
R6319 |
T9534 |
T9532 |
pobj |
mutagenesis,by |
R6320 |
T9535 |
T9534 |
prep |
with,mutagenesis |
R6321 |
T9536 |
T9537 |
det |
the,vector |
R6322 |
T9537 |
T9535 |
pobj |
vector,with |
R6323 |
T9538 |
T9537 |
compound |
gene,vector |
R6324 |
T9539 |
T9537 |
compound |
trap,vector |
R6325 |
T9540 |
T9537 |
punct |
", ",vector |
R6326 |
T9541 |
T9537 |
appos |
pGT0pfs,vector |
R6327 |
T9542 |
T9543 |
punct |
[,68 |
R6328 |
T9543 |
T9541 |
parataxis |
68,pGT0pfs |
R6329 |
T9544 |
T9543 |
nummod |
67,68 |
R6330 |
T9545 |
T9543 |
punct |
",",68 |
R6331 |
T9546 |
T9543 |
punct |
],68 |
R6332 |
T9547 |
T9537 |
punct |
(,vector |
R6333 |
T9548 |
T9537 |
acl |
generated,vector |
R6334 |
T9549 |
T9548 |
agent |
by,generated |
R6335 |
T9550 |
T9551 |
compound |
W.,Skarnes |
R6336 |
T9551 |
T9549 |
pobj |
Skarnes,by |
R6337 |
T9552 |
T9518 |
punct |
),obtained |
R6338 |
T9553 |
T9518 |
punct |
", ",obtained |
R6339 |
T9554 |
T9518 |
auxpass |
was,obtained |
R6340 |
T9555 |
T9518 |
prep |
from,obtained |
R6341 |
T9556 |
T9557 |
det |
the,Center |
R6342 |
T9557 |
T9555 |
pobj |
Center,from |
R6343 |
T9558 |
T9559 |
compound |
Mutant,Mouse |
R6344 |
T9559 |
T9557 |
compound |
Mouse,Center |
R6345 |
T9560 |
T9557 |
compound |
Regional,Center |
R6346 |
T9561 |
T9557 |
compound |
Resource,Center |
R6347 |
T9562 |
T9563 |
punct |
(,University |
R6348 |
T9563 |
T9557 |
parataxis |
University,Center |
R6349 |
T9564 |
T9563 |
prep |
of,University |
R6350 |
T9565 |
T9566 |
compound |
California,Davis |
R6351 |
T9566 |
T9564 |
pobj |
Davis,of |
R6352 |
T9567 |
T9563 |
punct |
", ",University |
R6353 |
T9568 |
T9563 |
npadvmod |
Davis,University |
R6354 |
T9569 |
T9563 |
punct |
", ",University |
R6355 |
T9570 |
T9563 |
npadvmod |
California,University |
R6356 |
T9571 |
T9563 |
punct |
", ",University |
R6357 |
T9572 |
T9573 |
compound |
United,States |
R6358 |
T9573 |
T9563 |
npadvmod |
States,University |
R6359 |
T9574 |
T9563 |
punct |
),University |
R6360 |
T9575 |
T9518 |
punct |
.,obtained |
R6361 |
T9577 |
T9578 |
det |
The,vector |
R6362 |
T9578 |
T9581 |
nsubj |
vector,contains |
R6363 |
T9579 |
T9580 |
compound |
gene,trap |
R6364 |
T9580 |
T9578 |
compound |
trap,vector |
R6365 |
T9582 |
T9583 |
det |
a,gene |
R6366 |
T9583 |
T9581 |
dobj |
gene,contains |
R6367 |
T9584 |
T9583 |
amod |
promoterless,gene |
R6368 |
T9585 |
T9583 |
compound |
neo-lacZ,gene |
R6369 |
T9586 |
T9583 |
compound |
fusion,gene |
R6370 |
T9587 |
T9583 |
prep |
with,gene |
R6371 |
T9588 |
T9589 |
det |
a,site |
R6372 |
T9589 |
T9587 |
pobj |
site,with |
R6373 |
T9590 |
T9589 |
compound |
splice,site |
R6374 |
T9591 |
T9589 |
compound |
acceptor,site |
R6375 |
T9592 |
T9589 |
prep |
at,site |
R6376 |
T9593 |
T9594 |
det |
the,end |
R6377 |
T9594 |
T9592 |
pobj |
end,at |
R6378 |
T9595 |
T9594 |
nummod |
5,end |
R6379 |
T9596 |
T9595 |
punct |
′,5 |
R6380 |
T9597 |
T9589 |
acl |
followed,site |
R6381 |
T9598 |
T9597 |
agent |
by,followed |
R6382 |
T9599 |
T9600 |
det |
an,site |
R6383 |
T9600 |
T9598 |
pobj |
site,by |
R6384 |
T9601 |
T9600 |
amod |
internal,site |
R6385 |
T9602 |
T9600 |
compound |
ribosome,site |
R6386 |
T9603 |
T9600 |
compound |
entry,site |
R6387 |
T9604 |
T9600 |
punct |
(,site |
R6388 |
T9605 |
T9600 |
appos |
IRES,site |
R6389 |
T9606 |
T9600 |
punct |
),site |
R6390 |
T9607 |
T9600 |
cc |
and,site |
R6391 |
T9608 |
T9609 |
det |
the,cassette |
R6392 |
T9609 |
T9600 |
conj |
cassette,site |
R6393 |
T9610 |
T9609 |
amod |
human,cassette |
R6394 |
T9611 |
T9612 |
amod |
placental,phosphatase |
R6395 |
T9612 |
T9609 |
nmod |
phosphatase,cassette |
R6396 |
T9613 |
T9612 |
nmod |
alkaline,phosphatase |
R6397 |
T9614 |
T9612 |
punct |
(,phosphatase |
R6398 |
T9615 |
T9612 |
appos |
PLAP,phosphatase |
R6399 |
T9616 |
T9609 |
punct |
),cassette |
R6400 |
T9617 |
T9609 |
compound |
reporter,cassette |
R6401 |
T9618 |
T9581 |
punct |
.,contains |
R6402 |
T9620 |
T9621 |
det |
The,line |
R6403 |
T9621 |
T9625 |
nsubjpass |
line,injected |
R6404 |
T9622 |
T9623 |
compound |
RanBP2,129Ola |
R6405 |
T9623 |
T9621 |
compound |
129Ola,line |
R6406 |
T9624 |
T9621 |
compound |
ES,line |
R6407 |
T9626 |
T9625 |
auxpass |
was,injected |
R6408 |
T9627 |
T9625 |
prep |
into,injected |
R6409 |
T9628 |
T9629 |
compound |
C57BL,6J |
R6410 |
T9629 |
T9631 |
compound |
6J,blastocysts |
R6411 |
T9630 |
T9629 |
punct |
/,6J |
R6412 |
T9631 |
T9627 |
pobj |
blastocysts,into |
R6413 |
T9632 |
T9625 |
cc |
and,injected |
R6414 |
T9633 |
T9634 |
nummod |
four,chimeras |
R6415 |
T9634 |
T9636 |
nsubjpass |
chimeras,generated |
R6416 |
T9635 |
T9634 |
amod |
male,chimeras |
R6417 |
T9636 |
T9625 |
conj |
generated,injected |
R6418 |
T9637 |
T9636 |
auxpass |
were,generated |
R6419 |
T9638 |
T9636 |
punct |
.,generated |
R6420 |
T9640 |
T9641 |
nsubjpass |
These,bred |
R6421 |
T9642 |
T9641 |
auxpass |
were,bred |
R6422 |
T9643 |
T9641 |
prep |
into,bred |
R6423 |
T9644 |
T9645 |
nmod |
129P2,OlaHsd |
R6424 |
T9645 |
T9647 |
nmod |
OlaHsd,females |
R6425 |
T9646 |
T9645 |
punct |
/,OlaHsd |
R6426 |
T9647 |
T9643 |
pobj |
females,into |
R6427 |
T9648 |
T9649 |
punct |
(,Harlan |
R6428 |
T9649 |
T9645 |
parataxis |
Harlan,OlaHsd |
R6429 |
T9650 |
T9649 |
punct |
", ",Harlan |
R6430 |
T9651 |
T9649 |
npadvmod |
Indianapolis,Harlan |
R6431 |
T9652 |
T9649 |
punct |
", ",Harlan |
R6432 |
T9653 |
T9649 |
npadvmod |
Indiana,Harlan |
R6433 |
T9654 |
T9649 |
punct |
", ",Harlan |
R6434 |
T9655 |
T9656 |
compound |
United,States |
R6435 |
T9656 |
T9649 |
npadvmod |
States,Harlan |
R6436 |
T9657 |
T9649 |
punct |
),Harlan |
R6437 |
T9658 |
T9645 |
cc |
and,OlaHsd |
R6438 |
T9659 |
T9660 |
compound |
C57BL,6J |
R6439 |
T9660 |
T9645 |
conj |
6J,OlaHsd |
R6440 |
T9661 |
T9660 |
punct |
/,6J |
R6441 |
T9662 |
T9663 |
punct |
(,Laboratory |
R6442 |
T9663 |
T9660 |
parataxis |
Laboratory,6J |
R6443 |
T9664 |
T9663 |
compound |
Jackson,Laboratory |
R6444 |
T9665 |
T9663 |
punct |
", ",Laboratory |
R6445 |
T9666 |
T9667 |
compound |
Bar,Harbor |
R6446 |
T9667 |
T9663 |
npadvmod |
Harbor,Laboratory |
R6447 |
T9668 |
T9663 |
punct |
", ",Laboratory |
R6448 |
T9669 |
T9663 |
npadvmod |
Maine,Laboratory |
R6449 |
T9670 |
T9663 |
punct |
", ",Laboratory |
R6450 |
T9671 |
T9672 |
compound |
United,States |
R6451 |
T9672 |
T9663 |
npadvmod |
States,Laboratory |
R6452 |
T9673 |
T9663 |
punct |
),Laboratory |
R6453 |
T9674 |
T9641 |
punct |
.,bred |
R6454 |
T9676 |
T9677 |
det |
The,lines |
R6455 |
T9677 |
T9683 |
nsubjpass |
lines,tested |
R6456 |
T9678 |
T9677 |
nummod |
two,lines |
R6457 |
T9679 |
T9677 |
amod |
resulting,lines |
R6458 |
T9680 |
T9679 |
cc |
and,resulting |
R6459 |
T9681 |
T9679 |
conj |
independent,resulting |
R6460 |
T9682 |
T9677 |
compound |
F1s,lines |
R6461 |
T9684 |
T9677 |
punct |
", ",lines |
R6462 |
T9685 |
T9677 |
prep |
with,lines |
R6463 |
T9686 |
T9687 |
compound |
129P2,OlaHsd |
R6464 |
T9687 |
T9685 |
pobj |
OlaHsd,with |
R6465 |
T9688 |
T9687 |
punct |
/,OlaHsd |
R6466 |
T9689 |
T9687 |
amod |
inbred,OlaHsd |
R6467 |
T9690 |
T9687 |
cc |
and,OlaHsd |
R6468 |
T9691 |
T9692 |
nmod |
129P2,6J |
R6469 |
T9692 |
T9698 |
nmod |
6J,backgrounds |
R6470 |
T9693 |
T9692 |
punct |
/,6J |
R6471 |
T9694 |
T9692 |
nmod |
OlaHsd,6J |
R6472 |
T9695 |
T9692 |
punct |
/,6J |
R6473 |
T9696 |
T9692 |
nmod |
C57BL,6J |
R6474 |
T9697 |
T9692 |
punct |
/,6J |
R6475 |
T9698 |
T9687 |
conj |
backgrounds,OlaHsd |
R6476 |
T9699 |
T9692 |
amod |
mixed,6J |
R6477 |
T9700 |
T9692 |
amod |
genetic,6J |
R6478 |
T9701 |
T9683 |
punct |
", ",tested |
R6479 |
T9702 |
T9683 |
auxpass |
were,tested |
R6480 |
T9703 |
T9683 |
prep |
for,tested |
R6481 |
T9704 |
T9705 |
compound |
germline,transmission |
R6482 |
T9705 |
T9703 |
pobj |
transmission,for |
R6483 |
T9706 |
T9683 |
prep |
by,tested |
R6484 |
T9707 |
T9708 |
nmod |
PCR,analysis |
R6485 |
T9708 |
T9706 |
pobj |
analysis,by |
R6486 |
T9709 |
T9707 |
cc |
and,PCR |
R6487 |
T9710 |
T9711 |
compound |
Southern,blot |
R6488 |
T9711 |
T9707 |
conj |
blot,PCR |
R6489 |
T9712 |
T9708 |
prep |
of,analysis |
R6490 |
T9713 |
T9714 |
nmod |
tail,DNA |
R6491 |
T9714 |
T9712 |
pobj |
DNA,of |
R6492 |
T9715 |
T9714 |
amod |
genomic,DNA |
R6493 |
T9716 |
T9683 |
punct |
.,tested |
R6494 |
T9718 |
T9719 |
nmod |
β,gal |
R6495 |
T9719 |
T9721 |
nmod |
gal,activities |
R6496 |
T9720 |
T9719 |
punct |
-,gal |
R6497 |
T9721 |
T9724 |
nsubjpass |
activities,detected |
R6498 |
T9722 |
T9719 |
cc |
and,gal |
R6499 |
T9723 |
T9719 |
conj |
PLAP,gal |
R6500 |
T9725 |
T9721 |
prep |
in,activities |
R6501 |
T9726 |
T9727 |
amod |
whole,mount |
R6502 |
T9727 |
T9728 |
compound |
mount,embryos |
R6503 |
T9728 |
T9725 |
pobj |
embryos,in |
R6504 |
T9729 |
T9728 |
cc |
and,embryos |
R6505 |
T9730 |
T9731 |
amod |
retinal,sections |
R6506 |
T9731 |
T9728 |
conj |
sections,embryos |
R6507 |
T9732 |
T9724 |
auxpass |
were,detected |
R6508 |
T9733 |
T9724 |
punct |
", ",detected |
R6509 |
T9734 |
T9724 |
advmod |
respectively,detected |
R6510 |
T9735 |
T9724 |
punct |
", ",detected |
R6511 |
T9736 |
T9724 |
prep |
by,detected |
R6512 |
T9737 |
T9736 |
pobj |
incubation,by |
R6513 |
T9738 |
T9737 |
prep |
with,incubation |
R6514 |
T9739 |
T9740 |
nummod |
5,bromo |
R6515 |
T9740 |
T9742 |
nmod |
bromo,galactopyranoside |
R6516 |
T9741 |
T9740 |
punct |
-,bromo |
R6517 |
T9742 |
T9755 |
nmod |
galactopyranoside,buffer |
R6518 |
T9743 |
T9742 |
punct |
-,galactopyranoside |
R6519 |
T9744 |
T9742 |
nummod |
4,galactopyranoside |
R6520 |
T9745 |
T9744 |
punct |
-,4 |
R6521 |
T9746 |
T9742 |
nmod |
chloro,galactopyranoside |
R6522 |
T9747 |
T9742 |
punct |
-,galactopyranoside |
R6523 |
T9748 |
T9749 |
nummod |
3,β |
R6524 |
T9749 |
T9742 |
nmod |
β,galactopyranoside |
R6525 |
T9750 |
T9749 |
punct |
-,β |
R6526 |
T9751 |
T9749 |
nmod |
indolyl,β |
R6527 |
T9752 |
T9742 |
punct |
-,galactopyranoside |
R6528 |
T9753 |
T9742 |
nmod |
D,galactopyranoside |
R6529 |
T9754 |
T9742 |
punct |
-,galactopyranoside |
R6530 |
T9755 |
T9738 |
pobj |
buffer,with |
R6531 |
T9756 |
T9742 |
cc |
and,galactopyranoside |
R6532 |
T9757 |
T9742 |
conj |
AP,galactopyranoside |
R6533 |
T9758 |
T9755 |
compound |
staining,buffer |
R6534 |
T9759 |
T9760 |
punct |
(,tetrazolium |
R6535 |
T9760 |
T9737 |
parataxis |
tetrazolium,incubation |
R6536 |
T9761 |
T9762 |
nummod |
0.1,mg |
R6537 |
T9762 |
T9763 |
nmod |
mg,phosphate |
R6538 |
T9763 |
T9760 |
dep |
phosphate,tetrazolium |
R6539 |
T9764 |
T9765 |
punct |
/,ml |
R6540 |
T9765 |
T9762 |
prep |
ml,mg |
R6541 |
T9766 |
T9767 |
nummod |
5,bromo |
R6542 |
T9767 |
T9763 |
nmod |
bromo,phosphate |
R6543 |
T9768 |
T9767 |
punct |
-,bromo |
R6544 |
T9769 |
T9763 |
punct |
-,phosphate |
R6545 |
T9770 |
T9771 |
nummod |
4,chloro |
R6546 |
T9771 |
T9763 |
nmod |
chloro,phosphate |
R6547 |
T9772 |
T9771 |
punct |
-,chloro |
R6548 |
T9773 |
T9763 |
punct |
-,phosphate |
R6549 |
T9774 |
T9763 |
nummod |
3,phosphate |
R6550 |
T9775 |
T9763 |
punct |
-,phosphate |
R6551 |
T9776 |
T9763 |
compound |
indolyl,phosphate |
R6552 |
T9777 |
T9760 |
punct |
", ",tetrazolium |
R6553 |
T9778 |
T9779 |
nummod |
1,mg |
R6554 |
T9779 |
T9760 |
nmod |
mg,tetrazolium |
R6555 |
T9780 |
T9781 |
punct |
/,ml |
R6556 |
T9781 |
T9779 |
prep |
ml,mg |
R6557 |
T9782 |
T9760 |
amod |
nitroblue,tetrazolium |
R6558 |
T9783 |
T9760 |
prep |
in,tetrazolium |
R6559 |
T9784 |
T9785 |
nummod |
100,mM |
R6560 |
T9785 |
T9786 |
compound |
mM,HCl |
R6561 |
T9786 |
T9783 |
pobj |
HCl,in |
R6562 |
T9787 |
T9786 |
compound |
Tris,HCl |
R6563 |
T9788 |
T9786 |
punct |
-,HCl |
R6564 |
T9789 |
T9790 |
punct |
(,pH |
R6565 |
T9790 |
T9786 |
parataxis |
pH,HCl |
R6566 |
T9791 |
T9790 |
nummod |
9.5,pH |
R6567 |
T9792 |
T9790 |
punct |
),pH |
R6568 |
T9793 |
T9786 |
punct |
", ",HCl |
R6569 |
T9794 |
T9795 |
nummod |
100,mM |
R6570 |
T9795 |
T9796 |
compound |
mM,NaCl |
R6571 |
T9796 |
T9786 |
appos |
NaCl,HCl |
R6572 |
T9797 |
T9786 |
punct |
", ",HCl |
R6573 |
T9798 |
T9799 |
nummod |
5,mM |
R6574 |
T9799 |
T9800 |
compound |
mM,MgCl2 |
R6575 |
T9800 |
T9786 |
appos |
MgCl2,HCl |
R6576 |
T9801 |
T9760 |
punct |
),tetrazolium |
R6577 |
T9802 |
T9803 |
mark |
as,described |
R6578 |
T9803 |
T9724 |
advcl |
described,detected |
R6579 |
T9804 |
T9803 |
advmod |
elsewhere,described |
R6580 |
T9805 |
T9806 |
punct |
[,68 |
R6581 |
T9806 |
T9803 |
parataxis |
68,described |
R6582 |
T9807 |
T9806 |
nummod |
67,68 |
R6583 |
T9808 |
T9806 |
punct |
",",68 |
R6584 |
T9809 |
T9806 |
punct |
],68 |
R6585 |
T9810 |
T9724 |
punct |
.,detected |
R6586 |
T9862 |
T9863 |
compound |
Western,blot |
R6587 |
T9864 |
T9863 |
cc |
and,blot |
R6588 |
T9865 |
T9866 |
compound |
quantitation,analysis |
R6589 |
T9866 |
T9863 |
conj |
analysis,blot |
R6590 |
T9867 |
T9863 |
prep |
of,blot |
R6591 |
T9868 |
T9869 |
compound |
protein,expression |
R6592 |
T9869 |
T9867 |
pobj |
expression,of |
R6593 |
T9870 |
T9869 |
cc |
and,expression |
R6594 |
T9871 |
T9872 |
compound |
ATP,levels |
R6595 |
T9872 |
T9869 |
conj |
levels,expression |
R6596 |
T9873 |
T9863 |
punct |
.,blot |
R6597 |
T9875 |
T9876 |
nsubjpass |
Homogenates,prepared |
R6598 |
T9877 |
T9875 |
cc |
and,Homogenates |
R6599 |
T9878 |
T9879 |
npadvmod |
detergent,solubilized |
R6600 |
T9879 |
T9880 |
amod |
solubilized,extracts |
R6601 |
T9880 |
T9875 |
conj |
extracts,Homogenates |
R6602 |
T9881 |
T9875 |
prep |
of,Homogenates |
R6603 |
T9882 |
T9881 |
pobj |
tissues,of |
R6604 |
T9883 |
T9882 |
cc |
and,tissues |
R6605 |
T9884 |
T9882 |
conj |
immunoblots,tissues |
R6606 |
T9885 |
T9876 |
auxpass |
were,prepared |
R6607 |
T9886 |
T9887 |
mark |
as,described |
R6608 |
T9887 |
T9876 |
advcl |
described,prepared |
R6609 |
T9888 |
T9887 |
advmod |
elsewhere,described |
R6610 |
T9889 |
T9890 |
punct |
[,69 |
R6611 |
T9890 |
T9887 |
parataxis |
69,described |
R6612 |
T9891 |
T9890 |
punct |
],69 |
R6613 |
T9892 |
T9876 |
punct |
.,prepared |
R6614 |
T9894 |
T9895 |
nsubjpass |
Quantitation,carried |
R6615 |
T9896 |
T9894 |
prep |
of,Quantitation |
R6616 |
T9897 |
T9898 |
compound |
immunoblot,bands |
R6617 |
T9898 |
T9896 |
pobj |
bands,of |
R6618 |
T9899 |
T9894 |
acl |
calibrated,Quantitation |
R6619 |
T9900 |
T9899 |
prep |
against,calibrated |
R6620 |
T9901 |
T9902 |
amod |
internal,bands |
R6621 |
T9902 |
T9900 |
pobj |
bands,against |
R6622 |
T9903 |
T9902 |
compound |
marker,bands |
R6623 |
T9904 |
T9902 |
punct |
", ",bands |
R6624 |
T9905 |
T9906 |
amod |
such,as |
R6625 |
T9906 |
T9902 |
prep |
as,bands |
R6626 |
T9907 |
T9906 |
pobj |
mHsp70,as |
R6627 |
T9908 |
T9907 |
cc |
and,mHsp70 |
R6628 |
T9909 |
T9907 |
conj |
Nup153,mHsp70 |
R6629 |
T9910 |
T9895 |
punct |
", ",carried |
R6630 |
T9911 |
T9895 |
auxpass |
was,carried |
R6631 |
T9912 |
T9895 |
prt |
out,carried |
R6632 |
T9913 |
T9895 |
prep |
with,carried |
R6633 |
T9914 |
T9915 |
compound |
Gel,Analyzer |
R6634 |
T9915 |
T9913 |
pobj |
Analyzer,with |
R6635 |
T9916 |
T9915 |
compound |
Pro,Analyzer |
R6636 |
T9917 |
T9918 |
punct |
(,MediaCybernetics |
R6637 |
T9918 |
T9915 |
parataxis |
MediaCybernetics,Analyzer |
R6638 |
T9919 |
T9918 |
punct |
", ",MediaCybernetics |
R6639 |
T9920 |
T9921 |
compound |
San,Diego |
R6640 |
T9921 |
T9918 |
npadvmod |
Diego,MediaCybernetics |
R6641 |
T9922 |
T9918 |
punct |
", ",MediaCybernetics |
R6642 |
T9923 |
T9918 |
npadvmod |
California,MediaCybernetics |
R6643 |
T9924 |
T9918 |
punct |
", ",MediaCybernetics |
R6644 |
T9925 |
T9926 |
compound |
United,States |
R6645 |
T9926 |
T9918 |
npadvmod |
States,MediaCybernetics |
R6646 |
T9927 |
T9918 |
punct |
),MediaCybernetics |
R6647 |
T9928 |
T9895 |
punct |
.,carried |
R6648 |
T9930 |
T9931 |
prep |
For,homogenized |
R6649 |
T9932 |
T9930 |
pobj |
determination,For |
R6650 |
T9933 |
T9932 |
prep |
of,determination |
R6651 |
T9934 |
T9935 |
compound |
ATP,levels |
R6652 |
T9935 |
T9933 |
pobj |
levels,of |
R6653 |
T9936 |
T9931 |
punct |
", ",homogenized |
R6654 |
T9937 |
T9938 |
advmod |
freshly,dissected |
R6655 |
T9938 |
T9939 |
amod |
dissected,tissues |
R6656 |
T9939 |
T9931 |
nsubjpass |
tissues,homogenized |
R6657 |
T9940 |
T9938 |
cc |
and,dissected |
R6658 |
T9941 |
T9942 |
npadvmod |
flash,frozen |
R6659 |
T9942 |
T9938 |
conj |
frozen,dissected |
R6660 |
T9943 |
T9942 |
punct |
-,frozen |
R6661 |
T9944 |
T9931 |
auxpass |
were,homogenized |
R6662 |
T9945 |
T9931 |
prep |
in,homogenized |
R6663 |
T9946 |
T9947 |
nummod |
2.5,% |
R6664 |
T9947 |
T9945 |
pobj |
%,in |
R6665 |
T9948 |
T9949 |
amod |
trichloroacetic,acid |
R6666 |
T9949 |
T9947 |
appos |
acid,% |
R6667 |
T9950 |
T9949 |
punct |
(,acid |
R6668 |
T9951 |
T9949 |
appos |
TCA,acid |
R6669 |
T9952 |
T9931 |
punct |
),homogenized |
R6670 |
T9953 |
T9931 |
punct |
", ",homogenized |
R6671 |
T9954 |
T9931 |
conj |
neutralized,homogenized |
R6672 |
T9955 |
T9954 |
punct |
", ",neutralized |
R6673 |
T9956 |
T9954 |
cc |
and,neutralized |
R6674 |
T9957 |
T9954 |
conj |
diluted,neutralized |
R6675 |
T9958 |
T9957 |
prep |
to,diluted |
R6676 |
T9959 |
T9960 |
det |
a,concentration |
R6677 |
T9960 |
T9958 |
pobj |
concentration,to |
R6678 |
T9961 |
T9960 |
amod |
final,concentration |
R6679 |
T9962 |
T9960 |
prep |
of,concentration |
R6680 |
T9963 |
T9964 |
nummod |
0.1,% |
R6681 |
T9964 |
T9962 |
pobj |
%,of |
R6682 |
T9965 |
T9957 |
prep |
with,diluted |
R6683 |
T9966 |
T9967 |
compound |
Tris,Acetate |
R6684 |
T9967 |
T9965 |
pobj |
Acetate,with |
R6685 |
T9968 |
T9967 |
punct |
-,Acetate |
R6686 |
T9969 |
T9970 |
punct |
(,pH |
R6687 |
T9970 |
T9967 |
parataxis |
pH,Acetate |
R6688 |
T9971 |
T9970 |
nummod |
7.75,pH |
R6689 |
T9972 |
T9970 |
punct |
),pH |
R6690 |
T9973 |
T9931 |
punct |
.,homogenized |
R6691 |
T9975 |
T9976 |
compound |
ATP,measurements |
R6692 |
T9976 |
T9977 |
nsubjpass |
measurements,carried |
R6693 |
T9978 |
T9977 |
auxpass |
were,carried |
R6694 |
T9979 |
T9977 |
prt |
out,carried |
R6695 |
T9980 |
T9977 |
prep |
with,carried |
R6696 |
T9981 |
T9982 |
det |
the,Kit |
R6697 |
T9982 |
T9980 |
pobj |
Kit,with |
R6698 |
T9983 |
T9982 |
compound |
ENLITEN,Kit |
R6699 |
T9984 |
T9985 |
compound |
ATP,System |
R6700 |
T9985 |
T9982 |
compound |
System,Kit |
R6701 |
T9986 |
T9985 |
compound |
Assay,System |
R6702 |
T9987 |
T9988 |
compound |
Bioluminescence,Detection |
R6703 |
T9988 |
T9982 |
compound |
Detection,Kit |
R6704 |
T9989 |
T9982 |
prep |
for,Kit |
R6705 |
T9990 |
T9989 |
pobj |
ATP,for |
R6706 |
T9991 |
T9992 |
punct |
(,Promega |
R6707 |
T9992 |
T9982 |
parataxis |
Promega,Kit |
R6708 |
T9993 |
T9992 |
punct |
", ",Promega |
R6709 |
T9994 |
T9992 |
npadvmod |
Madison,Promega |
R6710 |
T9995 |
T9992 |
punct |
", ",Promega |
R6711 |
T9996 |
T9992 |
npadvmod |
Wisconsin,Promega |
R6712 |
T9997 |
T9992 |
punct |
", ",Promega |
R6713 |
T9998 |
T9999 |
compound |
United,States |
R6714 |
T9999 |
T9992 |
npadvmod |
States,Promega |
R6715 |
T10000 |
T9992 |
punct |
),Promega |
R6716 |
T10001 |
T9977 |
prep |
as,carried |
R6717 |
T10002 |
T10001 |
prep |
per,as |
R6718 |
T10003 |
T10004 |
det |
the,manufacturer |
R6719 |
T10004 |
T10005 |
poss |
manufacturer,instruction |
R6720 |
T10005 |
T10002 |
pobj |
instruction,per |
R6721 |
T10006 |
T10004 |
case |
's,manufacturer |
R6722 |
T10007 |
T9977 |
prep |
in,carried |
R6723 |
T10008 |
T10009 |
compound |
SpectraMax,M5 |
R6724 |
T10009 |
T10007 |
pobj |
M5,in |
R6725 |
T10010 |
T10009 |
punct |
-,M5 |
R6726 |
T10011 |
T10012 |
punct |
(,Devices |
R6727 |
T10012 |
T10009 |
parataxis |
Devices,M5 |
R6728 |
T10013 |
T10012 |
compound |
Molecular,Devices |
R6729 |
T10014 |
T10012 |
punct |
", ",Devices |
R6730 |
T10015 |
T10012 |
npadvmod |
Sunnyvale,Devices |
R6731 |
T10016 |
T10012 |
punct |
", ",Devices |
R6732 |
T10017 |
T10012 |
npadvmod |
California,Devices |
R6733 |
T10018 |
T10012 |
punct |
", ",Devices |
R6734 |
T10019 |
T10020 |
compound |
United,States |
R6735 |
T10020 |
T10012 |
npadvmod |
States,Devices |
R6736 |
T10021 |
T10012 |
punct |
),Devices |
R6737 |
T10022 |
T9977 |
punct |
.,carried |
R6738 |
T10024 |
T10025 |
compound |
Protein,concentration |
R6739 |
T10025 |
T10026 |
nsubjpass |
concentration,measured |
R6740 |
T10027 |
T10026 |
auxpass |
was,measured |
R6741 |
T10028 |
T10026 |
prep |
by,measured |
R6742 |
T10029 |
T10030 |
det |
the,method |
R6743 |
T10030 |
T10028 |
pobj |
method,by |
R6744 |
T10031 |
T10030 |
compound |
BCA,method |
R6745 |
T10032 |
T10033 |
punct |
(,BioRad |
R6746 |
T10033 |
T10030 |
parataxis |
BioRad,method |
R6747 |
T10034 |
T10033 |
punct |
", ",BioRad |
R6748 |
T10035 |
T10033 |
npadvmod |
Hercules,BioRad |
R6749 |
T10036 |
T10033 |
punct |
", ",BioRad |
R6750 |
T10037 |
T10033 |
npadvmod |
California,BioRad |
R6751 |
T10038 |
T10033 |
punct |
", ",BioRad |
R6752 |
T10039 |
T10040 |
compound |
United,States |
R6753 |
T10040 |
T10033 |
npadvmod |
States,BioRad |
R6754 |
T10041 |
T10033 |
punct |
),BioRad |
R6755 |
T10042 |
T10026 |
advcl |
using,measured |
R6756 |
T10043 |
T10042 |
dobj |
BSA,using |
R6757 |
T10044 |
T10042 |
prep |
as,using |
R6758 |
T10045 |
T10046 |
det |
a,standard |
R6759 |
T10046 |
T10044 |
pobj |
standard,as |
R6760 |
T10047 |
T10026 |
punct |
.,measured |
R6761 |
T10131 |
T10132 |
amod |
Metabolic,assays |
R6762 |
T10133 |
T10132 |
punct |
.,assays |
R6763 |
T10135 |
T10136 |
nummod |
Two,groups |
R6764 |
T10136 |
T10137 |
nsubjpass |
groups,kept |
R6765 |
T10138 |
T10136 |
prep |
of,groups |
R6766 |
T10139 |
T10140 |
amod |
male,mice |
R6767 |
T10140 |
T10138 |
pobj |
mice,of |
R6768 |
T10141 |
T10137 |
auxpass |
were,kept |
R6769 |
T10142 |
T10137 |
prep |
under,kept |
R6770 |
T10143 |
T10144 |
amod |
separate,diet |
R6771 |
T10144 |
T10142 |
pobj |
diet,under |
R6772 |
T10145 |
T10137 |
prep |
since,kept |
R6773 |
T10146 |
T10145 |
pobj |
gestation,since |
R6774 |
T10147 |
T10146 |
cc |
or,gestation |
R6775 |
T10148 |
T10146 |
conj |
birth,gestation |
R6776 |
T10149 |
T10137 |
punct |
.,kept |
R6777 |
T10151 |
T10152 |
nummod |
One,group |
R6778 |
T10152 |
T10153 |
nsubjpass |
group,fed |
R6779 |
T10154 |
T10153 |
auxpass |
was,fed |
R6780 |
T10155 |
T10153 |
prep |
with,fed |
R6781 |
T10156 |
T10157 |
det |
a,diet |
R6782 |
T10157 |
T10155 |
pobj |
diet,with |
R6783 |
T10158 |
T10157 |
amod |
normal,diet |
R6784 |
T10159 |
T10157 |
compound |
chow,diet |
R6785 |
T10160 |
T10157 |
punct |
(,diet |
R6786 |
T10161 |
T10162 |
compound |
PicoLab,Diet |
R6787 |
T10162 |
T10157 |
appos |
Diet,diet |
R6788 |
T10163 |
T10162 |
compound |
Rodent,Diet |
R6789 |
T10164 |
T10162 |
nummod |
20,Diet |
R6790 |
T10165 |
T10162 |
punct |
−,Diet |
R6791 |
T10166 |
T10162 |
nummod |
5053,Diet |
R6792 |
T10167 |
T10168 |
punct |
;,LabDiet |
R6793 |
T10168 |
T10157 |
parataxis |
LabDiet,diet |
R6794 |
T10169 |
T10168 |
punct |
", ",LabDiet |
R6795 |
T10170 |
T10168 |
npadvmod |
Richmond,LabDiet |
R6796 |
T10171 |
T10168 |
punct |
", ",LabDiet |
R6797 |
T10172 |
T10168 |
npadvmod |
Indiana,LabDiet |
R6798 |
T10173 |
T10168 |
punct |
", ",LabDiet |
R6799 |
T10174 |
T10175 |
compound |
United,States |
R6800 |
T10175 |
T10168 |
npadvmod |
States,LabDiet |
R6801 |
T10176 |
T10168 |
punct |
),LabDiet |
R6802 |
T10177 |
T10153 |
punct |
", ",fed |
R6803 |
T10178 |
T10179 |
mark |
while,placed |
R6804 |
T10179 |
T10153 |
advcl |
placed,fed |
R6805 |
T10180 |
T10181 |
det |
the,other |
R6806 |
T10181 |
T10179 |
nsubjpass |
other,placed |
R6807 |
T10182 |
T10179 |
auxpass |
was,placed |
R6808 |
T10183 |
T10179 |
prep |
on,placed |
R6809 |
T10184 |
T10185 |
det |
a,diet |
R6810 |
T10185 |
T10183 |
pobj |
diet,on |
R6811 |
T10186 |
T10187 |
amod |
higher,energy |
R6812 |
T10187 |
T10185 |
nmod |
energy,diet |
R6813 |
T10188 |
T10187 |
cc |
and,energy |
R6814 |
T10189 |
T10187 |
conj |
fat,energy |
R6815 |
T10190 |
T10185 |
punct |
(,diet |
R6816 |
T10191 |
T10192 |
compound |
PicoLab,Diet |
R6817 |
T10192 |
T10185 |
appos |
Diet,diet |
R6818 |
T10193 |
T10192 |
compound |
Mouse,Diet |
R6819 |
T10194 |
T10192 |
nummod |
20,Diet |
R6820 |
T10195 |
T10192 |
punct |
−,Diet |
R6821 |
T10196 |
T10192 |
nummod |
5058,Diet |
R6822 |
T10197 |
T10198 |
punct |
;,LabDiet |
R6823 |
T10198 |
T10185 |
parataxis |
LabDiet,diet |
R6824 |
T10199 |
T10198 |
punct |
),LabDiet |
R6825 |
T10200 |
T10153 |
punct |
.,fed |
R6826 |
T10202 |
T10203 |
compound |
Glucose,tolerance |
R6827 |
T10203 |
T10204 |
compound |
tolerance,test |
R6828 |
T10204 |
T10205 |
nsubjpass |
test,performed |
R6829 |
T10206 |
T10205 |
auxpass |
was,performed |
R6830 |
T10207 |
T10205 |
prep |
on,performed |
R6831 |
T10208 |
T10209 |
amod |
overnight,fasted |
R6832 |
T10209 |
T10211 |
amod |
fasted,mice |
R6833 |
T10210 |
T10209 |
punct |
-,fasted |
R6834 |
T10211 |
T10207 |
pobj |
mice,on |
R6835 |
T10212 |
T10211 |
acl |
injected,mice |
R6836 |
T10213 |
T10212 |
advmod |
intraperitoneally,injected |
R6837 |
T10214 |
T10212 |
prep |
with,injected |
R6838 |
T10215 |
T10216 |
nummod |
2,g |
R6839 |
T10216 |
T10214 |
pobj |
g,with |
R6840 |
T10217 |
T10216 |
prep |
of,g |
R6841 |
T10218 |
T10217 |
pobj |
glucose,of |
R6842 |
T10219 |
T10216 |
prep |
per,g |
R6843 |
T10220 |
T10219 |
pobj |
kg,per |
R6844 |
T10221 |
T10220 |
prep |
of,kg |
R6845 |
T10222 |
T10223 |
compound |
body,weight |
R6846 |
T10223 |
T10221 |
pobj |
weight,of |
R6847 |
T10224 |
T10205 |
punct |
.,performed |
R6848 |
T10226 |
T10227 |
amod |
Venous,values |
R6849 |
T10227 |
T10230 |
nsubjpass |
values,determined |
R6850 |
T10228 |
T10229 |
compound |
blood,glucose |
R6851 |
T10229 |
T10227 |
compound |
glucose,values |
R6852 |
T10231 |
T10230 |
auxpass |
were,determined |
R6853 |
T10232 |
T10230 |
prep |
at,determined |
R6854 |
T10233 |
T10234 |
advmod |
immediately,before |
R6855 |
T10234 |
T10232 |
prep |
before,at |
R6856 |
T10235 |
T10236 |
punct |
(,min |
R6857 |
T10236 |
T10234 |
parataxis |
min,before |
R6858 |
T10237 |
T10236 |
nummod |
0,min |
R6859 |
T10238 |
T10236 |
punct |
),min |
R6860 |
T10239 |
T10234 |
punct |
", ",before |
R6861 |
T10240 |
T10234 |
cc |
and,before |
R6862 |
T10241 |
T10242 |
nummod |
15,min |
R6863 |
T10242 |
T10250 |
npadvmod |
min,injection |
R6864 |
T10243 |
T10241 |
punct |
", ",15 |
R6865 |
T10244 |
T10241 |
conj |
30,15 |
R6866 |
T10245 |
T10244 |
punct |
", ",30 |
R6867 |
T10246 |
T10244 |
conj |
60,30 |
R6868 |
T10247 |
T10246 |
punct |
", ",60 |
R6869 |
T10248 |
T10246 |
cc |
and,60 |
R6870 |
T10249 |
T10246 |
conj |
120,60 |
R6871 |
T10250 |
T10234 |
conj |
injection,before |
R6872 |
T10251 |
T10242 |
prep |
after,min |
R6873 |
T10252 |
T10250 |
det |
the,injection |
R6874 |
T10253 |
T10250 |
prep |
with,injection |
R6875 |
T10254 |
T10255 |
det |
an,instrument |
R6876 |
T10255 |
T10253 |
pobj |
instrument,with |
R6877 |
T10256 |
T10255 |
amod |
automatic,instrument |
R6878 |
T10257 |
T10255 |
compound |
glucose,instrument |
R6879 |
T10258 |
T10255 |
compound |
monitor,instrument |
R6880 |
T10259 |
T10255 |
punct |
(,instrument |
R6881 |
T10260 |
T10261 |
nummod |
One,Ultra |
R6882 |
T10261 |
T10255 |
appos |
Ultra,instrument |
R6883 |
T10262 |
T10261 |
compound |
Touch,Ultra |
R6884 |
T10263 |
T10264 |
punct |
", ",Lifescan |
R6885 |
T10264 |
T10255 |
parataxis |
Lifescan,instrument |
R6886 |
T10265 |
T10264 |
punct |
),Lifescan |
R6887 |
T10266 |
T10230 |
punct |
.,determined |
R6888 |
T10268 |
T10269 |
compound |
Insulin,tolerance |
R6889 |
T10269 |
T10270 |
compound |
tolerance,test |
R6890 |
T10270 |
T10271 |
nsubjpass |
test,performed |
R6891 |
T10272 |
T10271 |
auxpass |
was,performed |
R6892 |
T10273 |
T10274 |
det |
the,way |
R6893 |
T10274 |
T10271 |
npadvmod |
way,performed |
R6894 |
T10275 |
T10274 |
amod |
same,way |
R6895 |
T10276 |
T10274 |
prep |
as,way |
R6896 |
T10277 |
T10276 |
pobj |
that,as |
R6897 |
T10278 |
T10277 |
acl |
described,that |
R6898 |
T10279 |
T10278 |
prep |
for,described |
R6899 |
T10280 |
T10281 |
det |
the,test |
R6900 |
T10281 |
T10279 |
pobj |
test,for |
R6901 |
T10282 |
T10283 |
compound |
glucose,tolerance |
R6902 |
T10283 |
T10281 |
compound |
tolerance,test |
R6903 |
T10284 |
T10274 |
prep |
with,way |
R6904 |
T10285 |
T10286 |
det |
the,exception |
R6905 |
T10286 |
T10284 |
pobj |
exception,with |
R6906 |
T10287 |
T10288 |
mark |
that,injected |
R6907 |
T10288 |
T10286 |
acl |
injected,exception |
R6908 |
T10289 |
T10290 |
amod |
human,insulin |
R6909 |
T10290 |
T10288 |
nsubjpass |
insulin,injected |
R6910 |
T10291 |
T10292 |
punct |
(,U |
R6911 |
T10292 |
T10290 |
parataxis |
U,insulin |
R6912 |
T10293 |
T10292 |
nummod |
0.75,U |
R6913 |
T10294 |
T10295 |
punct |
/,kg |
R6914 |
T10295 |
T10292 |
prep |
kg,U |
R6915 |
T10296 |
T10295 |
prep |
of,kg |
R6916 |
T10297 |
T10298 |
compound |
body,mass |
R6917 |
T10298 |
T10296 |
pobj |
mass,of |
R6918 |
T10299 |
T10292 |
punct |
),U |
R6919 |
T10300 |
T10288 |
auxpass |
was,injected |
R6920 |
T10301 |
T10288 |
advmod |
intraperitoneally,injected |
R6921 |
T10302 |
T10288 |
prep |
in,injected |
R6922 |
T10303 |
T10304 |
amod |
non-fasted,mice |
R6923 |
T10304 |
T10302 |
pobj |
mice,in |
R6924 |
T10305 |
T10271 |
punct |
.,performed |
R6925 |
T10307 |
T10308 |
compound |
Pyruvate,challenge |
R6926 |
T10308 |
T10309 |
compound |
challenge,test |
R6927 |
T10309 |
T10310 |
nsubjpass |
test,performed |
R6928 |
T10311 |
T10310 |
auxpass |
was,performed |
R6929 |
T10312 |
T10313 |
det |
the,way |
R6930 |
T10313 |
T10310 |
npadvmod |
way,performed |
R6931 |
T10314 |
T10313 |
amod |
same,way |
R6932 |
T10315 |
T10313 |
prep |
as,way |
R6933 |
T10316 |
T10315 |
pobj |
that,as |
R6934 |
T10317 |
T10316 |
acl |
described,that |
R6935 |
T10318 |
T10317 |
prep |
for,described |
R6936 |
T10319 |
T10320 |
det |
the,test |
R6937 |
T10320 |
T10318 |
pobj |
test,for |
R6938 |
T10321 |
T10322 |
compound |
glucose,tolerance |
R6939 |
T10322 |
T10320 |
compound |
tolerance,test |
R6940 |
T10323 |
T10313 |
prep |
with,way |
R6941 |
T10324 |
T10325 |
det |
the,exception |
R6942 |
T10325 |
T10323 |
pobj |
exception,with |
R6943 |
T10326 |
T10327 |
mark |
that,injected |
R6944 |
T10327 |
T10325 |
acl |
injected,exception |
R6945 |
T10328 |
T10327 |
nsubjpass |
pyruvate,injected |
R6946 |
T10329 |
T10328 |
acl |
dissolved,pyruvate |
R6947 |
T10330 |
T10329 |
prep |
in,dissolved |
R6948 |
T10331 |
T10330 |
pobj |
saline,in |
R6949 |
T10332 |
T10333 |
punct |
(,g |
R6950 |
T10333 |
T10331 |
parataxis |
g,saline |
R6951 |
T10334 |
T10333 |
nummod |
2,g |
R6952 |
T10335 |
T10336 |
punct |
/,kg |
R6953 |
T10336 |
T10333 |
prep |
kg,g |
R6954 |
T10337 |
T10336 |
prep |
of,kg |
R6955 |
T10338 |
T10339 |
compound |
body,mass |
R6956 |
T10339 |
T10337 |
pobj |
mass,of |
R6957 |
T10340 |
T10333 |
punct |
),g |
R6958 |
T10341 |
T10327 |
auxpass |
was,injected |
R6959 |
T10342 |
T10327 |
advmod |
intraperitoneally,injected |
R6960 |
T10343 |
T10327 |
prep |
in,injected |
R6961 |
T10344 |
T10345 |
amod |
overnight,fasted |
R6962 |
T10345 |
T10347 |
amod |
fasted,mice |
R6963 |
T10346 |
T10345 |
punct |
-,fasted |
R6964 |
T10347 |
T10343 |
pobj |
mice,in |
R6965 |
T10348 |
T10310 |
punct |
.,performed |
R6966 |
T10408 |
T10407 |
punct |
.,Electroretinography |
R6967 |
T10410 |
T10411 |
nummod |
Five,mice |
R6968 |
T10411 |
T10428 |
nsubjpass |
mice,kept |
R6969 |
T10412 |
T10413 |
punct |
~,6 |
R6970 |
T10413 |
T10414 |
nummod |
6,mo |
R6971 |
T10414 |
T10416 |
npadvmod |
mo,old |
R6972 |
T10415 |
T10414 |
punct |
-,mo |
R6973 |
T10416 |
T10411 |
amod |
old,mice |
R6974 |
T10417 |
T10416 |
punct |
-,old |
R6975 |
T10418 |
T10411 |
amod |
male,mice |
R6976 |
T10419 |
T10411 |
nmod |
RanBP2,mice |
R6977 |
T10420 |
T10419 |
punct |
+,RanBP2 |
R6978 |
T10421 |
T10419 |
punct |
/,RanBP2 |
R6979 |
T10422 |
T10419 |
punct |
+,RanBP2 |
R6980 |
T10423 |
T10419 |
cc |
and,RanBP2 |
R6981 |
T10424 |
T10419 |
conj |
RanBP2,RanBP2 |
R6982 |
T10425 |
T10424 |
punct |
+,RanBP2 |
R6983 |
T10426 |
T10424 |
punct |
/,RanBP2 |
R6984 |
T10427 |
T10424 |
punct |
−,RanBP2 |
R6985 |
T10429 |
T10411 |
prep |
in,mice |
R6986 |
T10430 |
T10431 |
compound |
129ola,background |
R6987 |
T10431 |
T10429 |
pobj |
background,in |
R6988 |
T10432 |
T10428 |
auxpass |
were,kept |
R6989 |
T10433 |
T10428 |
prep |
in,kept |
R6990 |
T10434 |
T10435 |
nmod |
dim,light |
R6991 |
T10435 |
T10433 |
pobj |
light,in |
R6992 |
T10436 |
T10435 |
amod |
cyclic,light |
R6993 |
T10437 |
T10438 |
punct |
(,lux |
R6994 |
T10438 |
T10435 |
parataxis |
lux,light |
R6995 |
T10439 |
T10438 |
nummod |
10,lux |
R6996 |
T10440 |
T10438 |
punct |
),lux |
R6997 |
T10441 |
T10428 |
prep |
for,kept |
R6998 |
T10442 |
T10443 |
nummod |
2,wk |
R6999 |
T10443 |
T10441 |
pobj |
wk,for |
R7000 |
T10444 |
T10445 |
mark |
before,recorded |
R7001 |
T10445 |
T10428 |
advcl |
recorded,kept |
R7002 |
T10446 |
T10447 |
amod |
electroretinographic,responses |
R7003 |
T10447 |
T10445 |
nsubjpass |
responses,recorded |
R7004 |
T10448 |
T10445 |
auxpass |
were,recorded |
R7005 |
T10449 |
T10428 |
punct |
.,kept |
R7006 |
T10451 |
T10452 |
det |
The,mice |
R7007 |
T10452 |
T10453 |
nsubjpass |
mice,anesthetized |
R7008 |
T10454 |
T10453 |
auxpass |
were,anesthetized |
R7009 |
T10455 |
T10453 |
advmod |
intraperitoneally,anesthetized |
R7010 |
T10456 |
T10453 |
prep |
with,anesthetized |
R7011 |
T10457 |
T10456 |
pobj |
ketamine,with |
R7012 |
T10458 |
T10459 |
punct |
(,mg |
R7013 |
T10459 |
T10457 |
parataxis |
mg,ketamine |
R7014 |
T10460 |
T10459 |
nummod |
80,mg |
R7015 |
T10461 |
T10462 |
punct |
/,kg |
R7016 |
T10462 |
T10459 |
prep |
kg,mg |
R7017 |
T10463 |
T10459 |
punct |
),mg |
R7018 |
T10464 |
T10457 |
cc |
and,ketamine |
R7019 |
T10465 |
T10457 |
conj |
xylazine,ketamine |
R7020 |
T10466 |
T10467 |
punct |
(,mg |
R7021 |
T10467 |
T10465 |
parataxis |
mg,xylazine |
R7022 |
T10468 |
T10467 |
nummod |
4,mg |
R7023 |
T10469 |
T10470 |
punct |
/,kg |
R7024 |
T10470 |
T10467 |
prep |
kg,mg |
R7025 |
T10471 |
T10467 |
punct |
),mg |
R7026 |
T10472 |
T10453 |
punct |
.,anesthetized |
R7027 |
T10474 |
T10475 |
compound |
Body,temperature |
R7028 |
T10475 |
T10476 |
nsubjpass |
temperature,maintained |
R7029 |
T10477 |
T10476 |
auxpass |
was,maintained |
R7030 |
T10478 |
T10476 |
prep |
with,maintained |
R7031 |
T10479 |
T10480 |
det |
a,pad |
R7032 |
T10480 |
T10478 |
pobj |
pad,with |
R7033 |
T10481 |
T10480 |
compound |
heating,pad |
R7034 |
T10482 |
T10476 |
prep |
at,maintained |
R7035 |
T10483 |
T10484 |
quantmod |
37.5,1 |
R7036 |
T10484 |
T10486 |
nummod |
1,°C |
R7037 |
T10485 |
T10484 |
punct |
±,1 |
R7038 |
T10486 |
T10482 |
pobj |
°C,at |
R7039 |
T10487 |
T10476 |
prep |
throughout,maintained |
R7040 |
T10488 |
T10489 |
det |
the,recording |
R7041 |
T10489 |
T10487 |
pobj |
recording,throughout |
R7042 |
T10490 |
T10476 |
punct |
.,maintained |
R7043 |
T10492 |
T10493 |
det |
The,pupils |
R7044 |
T10493 |
T10494 |
nsubjpass |
pupils,dilated |
R7045 |
T10495 |
T10494 |
auxpass |
were,dilated |
R7046 |
T10496 |
T10494 |
advmod |
fully,dilated |
R7047 |
T10497 |
T10494 |
prep |
with,dilated |
R7048 |
T10498 |
T10499 |
nummod |
0.5,% |
R7049 |
T10499 |
T10500 |
compound |
%,tropicamide |
R7050 |
T10500 |
T10497 |
pobj |
tropicamide,with |
R7051 |
T10501 |
T10500 |
cc |
and,tropicamide |
R7052 |
T10502 |
T10503 |
nummod |
2.5,% |
R7053 |
T10503 |
T10504 |
compound |
%,HCl |
R7054 |
T10504 |
T10500 |
conj |
HCl,tropicamide |
R7055 |
T10505 |
T10504 |
compound |
phenylephrine,HCl |
R7056 |
T10506 |
T10494 |
punct |
", ",dilated |
R7057 |
T10507 |
T10494 |
cc |
and,dilated |
R7058 |
T10508 |
T10509 |
det |
both,eyes |
R7059 |
T10509 |
T10510 |
nsubjpass |
eyes,recorded |
R7060 |
T10510 |
T10494 |
conj |
recorded,dilated |
R7061 |
T10511 |
T10510 |
auxpass |
were,recorded |
R7062 |
T10512 |
T10510 |
advmod |
simultaneously,recorded |
R7063 |
T10513 |
T10510 |
punct |
.,recorded |
R7064 |
T10515 |
T10516 |
amod |
Corneal,loops |
R7065 |
T10516 |
T10520 |
nsubjpass |
loops,used |
R7066 |
T10517 |
T10518 |
amod |
gold,wire |
R7067 |
T10518 |
T10516 |
compound |
wire,loops |
R7068 |
T10519 |
T10518 |
punct |
-,wire |
R7069 |
T10521 |
T10520 |
auxpass |
were,used |
R7070 |
T10522 |
T10520 |
prep |
as,used |
R7071 |
T10523 |
T10524 |
det |
the,electrodes |
R7072 |
T10524 |
T10522 |
pobj |
electrodes,as |
R7073 |
T10525 |
T10524 |
amod |
active,electrodes |
R7074 |
T10526 |
T10520 |
prep |
under,used |
R7075 |
T10527 |
T10528 |
nummod |
0.5,% |
R7076 |
T10528 |
T10529 |
nmod |
%,hydrochloride |
R7077 |
T10529 |
T10531 |
nmod |
hydrochloride,anesthesia |
R7078 |
T10530 |
T10529 |
nmod |
proparacaine,hydrochloride |
R7079 |
T10531 |
T10526 |
pobj |
anesthesia,under |
R7080 |
T10532 |
T10531 |
amod |
topical,anesthesia |
R7081 |
T10533 |
T10520 |
punct |
.,used |
R7082 |
T10535 |
T10536 |
amod |
Gold,wire |
R7083 |
T10536 |
T10538 |
compound |
wire,electrodes |
R7084 |
T10537 |
T10536 |
punct |
-,wire |
R7085 |
T10538 |
T10539 |
nsubj |
electrodes,served |
R7086 |
T10540 |
T10538 |
acl |
placed,electrodes |
R7087 |
T10541 |
T10540 |
prep |
on,placed |
R7088 |
T10542 |
T10543 |
det |
the,sclera |
R7089 |
T10543 |
T10541 |
pobj |
sclera,on |
R7090 |
T10544 |
T10543 |
prep |
near,sclera |
R7091 |
T10545 |
T10546 |
det |
the,limbus |
R7092 |
T10546 |
T10544 |
pobj |
limbus,near |
R7093 |
T10547 |
T10539 |
prep |
as,served |
R7094 |
T10548 |
T10549 |
compound |
reference,electrodes |
R7095 |
T10549 |
T10547 |
pobj |
electrodes,as |
R7096 |
T10550 |
T10539 |
punct |
.,served |
R7097 |
T10552 |
T10553 |
det |
A,wire |
R7098 |
T10553 |
T10555 |
nsubjpass |
wire,attached |
R7099 |
T10554 |
T10553 |
compound |
ground,wire |
R7100 |
T10556 |
T10555 |
auxpass |
was,attached |
R7101 |
T10557 |
T10555 |
prep |
to,attached |
R7102 |
T10558 |
T10559 |
det |
the,ear |
R7103 |
T10559 |
T10557 |
pobj |
ear,to |
R7104 |
T10560 |
T10555 |
punct |
.,attached |
R7105 |
T10562 |
T10563 |
amod |
Scotopic,responses |
R7106 |
T10563 |
T10564 |
nsubjpass |
responses,recorded |
R7107 |
T10565 |
T10564 |
auxpass |
were,recorded |
R7108 |
T10566 |
T10564 |
prep |
from,recorded |
R7109 |
T10567 |
T10566 |
pobj |
threshold,from |
R7110 |
T10568 |
T10566 |
prep |
to,from |
R7111 |
T10569 |
T10570 |
nummod |
9,units |
R7112 |
T10570 |
T10568 |
pobj |
units,to |
R7113 |
T10571 |
T10570 |
compound |
log,units |
R7114 |
T10572 |
T10570 |
prep |
of,units |
R7115 |
T10573 |
T10572 |
pobj |
intensity,of |
R7116 |
T10574 |
T10570 |
prep |
above,units |
R7117 |
T10575 |
T10574 |
pobj |
threshold,above |
R7118 |
T10576 |
T10564 |
punct |
", ",recorded |
R7119 |
T10577 |
T10564 |
conj |
amplified,recorded |
R7120 |
T10578 |
T10577 |
punct |
", ",amplified |
R7121 |
T10579 |
T10577 |
cc |
and,amplified |
R7122 |
T10580 |
T10577 |
conj |
filtered,amplified |
R7123 |
T10581 |
T10582 |
punct |
(,Hz |
R7124 |
T10582 |
T10580 |
parataxis |
Hz,filtered |
R7125 |
T10583 |
T10584 |
nummod |
"5,000",gain |
R7126 |
T10584 |
T10582 |
dep |
gain,Hz |
R7127 |
T10585 |
T10582 |
punct |
", ",Hz |
R7128 |
T10586 |
T10587 |
quantmod |
0.1,"1,000" |
R7129 |
T10587 |
T10582 |
nummod |
"1,000",Hz |
R7130 |
T10588 |
T10587 |
punct |
–,"1,000" |
R7131 |
T10589 |
T10582 |
punct |
),Hz |
R7132 |
T10590 |
T10564 |
punct |
.,recorded |
R7133 |
T10592 |
T10593 |
prep |
For,used |
R7134 |
T10594 |
T10595 |
amod |
photopic,recordings |
R7135 |
T10595 |
T10592 |
pobj |
recordings,For |
R7136 |
T10596 |
T10597 |
compound |
background,light |
R7137 |
T10597 |
T10593 |
nsubjpass |
light,used |
R7138 |
T10598 |
T10597 |
prep |
at,light |
R7139 |
T10599 |
T10600 |
nummod |
34,cd |
R7140 |
T10600 |
T10598 |
pobj |
cd,at |
R7141 |
T10601 |
T10602 |
punct |
/,m2 |
R7142 |
T10602 |
T10600 |
prep |
m2,cd |
R7143 |
T10603 |
T10593 |
auxpass |
was,used |
R7144 |
T10604 |
T10605 |
aux |
to,suppress |
R7145 |
T10605 |
T10593 |
advcl |
suppress,used |
R7146 |
T10606 |
T10607 |
compound |
rod,function |
R7147 |
T10607 |
T10605 |
dobj |
function,suppress |
R7148 |
T10608 |
T10593 |
punct |
.,used |