PMC:1626108 / 41174-52203 JSON TXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id	Subject	Object	Predicate	Lexical cue
T7787	23-28	NN	denotes	Yeast
T7789	29-32	CD	denotes	two
T7791	32-33	HYPH	denotes	-
T7790	33-39	NN	denotes	hybrid
T7788	40-49	NN	denotes	screening
T7792	50-53	CC	denotes	and
T7793	54-60	NNS	denotes	assays
T7794	60-61	.	denotes	.
T7795	61-325	sentence	denotes	The LD of human RanBP2 (residues 62–711) was subcloned in-frame into the GAL4 DNA-binding domain of bait vector, pBUTE (a kanamycin-resistant version of GAL4 bait vector pGBDUC1) [62] and HybriZAP pBD-GAL4 vector (Stratagene, La Jolla, California, United States).
T7796	62-65	DT	denotes	The
T7797	66-68	NN	denotes	LD
T7799	69-71	IN	denotes	of
T7800	72-77	JJ	denotes	human
T7801	78-84	NN	denotes	RanBP2
T7802	85-86	-LRB-	denotes	(
T7804	86-94	NNS	denotes	residues
T7803	95-97	CD	denotes	62
T7805	97-98	SYM	denotes	–
T7806	98-101	CD	denotes	711
T7807	101-102	-RRB-	denotes	)
T7808	103-106	VBD	denotes	was
T7798	107-116	VBN	denotes	subcloned
T7809	117-119	IN	denotes	in
T7810	119-120	HYPH	denotes	-
T7811	120-125	NN	denotes	frame
T7812	126-130	IN	denotes	into
T7813	131-134	DT	denotes	the
T7815	135-139	NN	denotes	GAL4
T7816	140-143	NN	denotes	DNA
T7818	143-144	HYPH	denotes	-
T7817	144-151	NN	denotes	binding
T7814	152-158	NN	denotes	domain
T7819	159-161	IN	denotes	of
T7820	162-166	NN	denotes	bait
T7821	167-173	NN	denotes	vector
T7822	173-175	,	denotes	,
T7823	175-180	NN	denotes	pBUTE
T7824	181-182	-LRB-	denotes	(
T7825	182-183	DT	denotes	a
T7827	184-193	NN	denotes	kanamycin
T7829	193-194	HYPH	denotes	-
T7828	194-203	JJ	denotes	resistant
T7826	204-211	NN	denotes	version
T7830	212-214	IN	denotes	of
T7831	215-219	NN	denotes	GAL4
T7833	220-224	NN	denotes	bait
T7832	225-231	NN	denotes	vector
T7834	232-239	NN	denotes	pGBDUC1
T7835	239-240	-RRB-	denotes	)
T7836	241-242	-LRB-	denotes	[
T7837	242-244	CD	denotes	62
T7838	244-245	-RRB-	denotes	]
T7839	246-249	CC	denotes	and
T7840	250-258	NN	denotes	HybriZAP
T7842	259-262	NN	denotes	pBD
T7844	262-263	HYPH	denotes	-
T7843	263-267	NN	denotes	GAL4
T7841	268-274	NN	denotes	vector
T7845	275-276	-LRB-	denotes	(
T7846	276-286	NNP	denotes	Stratagene
T7847	286-288	,	denotes	,
T7848	288-290	NNP	denotes	La
T7849	291-296	NNP	denotes	Jolla
T7850	296-298	,	denotes	,
T7851	298-308	NNP	denotes	California
T7852	308-310	,	denotes	,
T7853	310-316	NNP	denotes	United
T7854	317-323	NNP	denotes	States
T7855	323-324	-RRB-	denotes	)
T7856	324-325	.	denotes	.
T7857	325-534	sentence	denotes	The former was used to screen ~18 million clones via mating from murine 9- to 10-d-old embryo and brain cDNA libraries at the Molecular Interaction Facility (MIF), University of Wisconsin, Madison, Wisconsin.
T7858	326-329	DT	denotes	The
T7859	330-336	JJ	denotes	former
T7861	337-340	VBD	denotes	was
T7860	341-345	VBN	denotes	used
T7862	346-348	TO	denotes	to
T7863	349-355	VB	denotes	screen
T7864	356-357	SYM	denotes	~
T7866	357-359	CD	denotes	18
T7865	360-367	CD	denotes	million
T7867	368-374	NNS	denotes	clones
T7868	375-378	IN	denotes	via
T7869	379-385	VBG	denotes	mating
T7870	386-390	IN	denotes	from
T7871	391-397	NN	denotes	murine
T7873	398-399	CD	denotes	9
T7875	399-400	HYPH	denotes	-
T7876	401-403	IN	denotes	to
T7874	404-406	CD	denotes	10
T7878	406-407	HYPH	denotes	-
T7877	407-408	NN	denotes	d
T7880	408-409	HYPH	denotes	-
T7879	409-412	JJ	denotes	old
T7881	413-419	NN	denotes	embryo
T7882	420-423	CC	denotes	and
T7883	424-429	NN	denotes	brain
T7884	430-434	NN	denotes	cDNA
T7872	435-444	NNS	denotes	libraries
T7885	445-447	IN	denotes	at
T7886	448-451	DT	denotes	the
T7888	452-461	NNP	denotes	Molecular
T7889	462-473	NNP	denotes	Interaction
T7887	474-482	NNP	denotes	Facility
T7890	483-484	-LRB-	denotes	(
T7891	484-487	NNP	denotes	MIF
T7892	487-488	-RRB-	denotes	)
T7893	488-490	,	denotes	,
T7894	490-500	NNP	denotes	University
T7895	501-503	IN	denotes	of
T7896	504-513	NNP	denotes	Wisconsin
T7897	513-515	,	denotes	,
T7898	515-522	NNP	denotes	Madison
T7899	522-524	,	denotes	,
T7900	524-533	NNP	denotes	Wisconsin
T7901	533-534	.	denotes	.
T7902	534-645	sentence	denotes	The latter was used to screen via transformation of ~5 million clones from bovine retinal cDNA libraries [32].
T7903	535-538	DT	denotes	The
T7904	539-545	JJ	denotes	latter
T7906	546-549	VBD	denotes	was
T7905	550-554	VBN	denotes	used
T7907	555-557	TO	denotes	to
T7908	558-564	VB	denotes	screen
T7909	565-568	IN	denotes	via
T7910	569-583	NN	denotes	transformation
T7911	584-586	IN	denotes	of
T7912	587-588	SYM	denotes	~
T7914	588-589	CD	denotes	5
T7913	590-597	CD	denotes	million
T7915	598-604	NNS	denotes	clones
T7916	605-609	IN	denotes	from
T7917	610-616	JJ	denotes	bovine
T7919	617-624	JJ	denotes	retinal
T7920	625-629	NN	denotes	cDNA
T7918	630-639	NNS	denotes	libraries
T7921	640-641	-LRB-	denotes	[
T7922	641-643	CD	denotes	32
T7923	643-644	-RRB-	denotes	]
T7924	644-645	.	denotes	.
T7925	645-679	sentence	denotes	The screens generated six clones.
T7926	646-649	DT	denotes	The
T7927	650-657	NNS	denotes	screens
T7928	658-667	VBD	denotes	generated
T7929	668-671	CD	denotes	six
T7930	672-678	NNS	denotes	clones
T7931	678-679	.	denotes	.
T7932	679-832	sentence	denotes	One and two in-frame clones were independently isolated from the embryonic and adult brain libraries, respectively, and the interactions were validated.
T7933	680-683	CD	denotes	One
T7935	684-687	CC	denotes	and
T7936	688-691	CD	denotes	two
T7937	692-694	IN	denotes	in
T7938	694-695	HYPH	denotes	-
T7939	695-700	NN	denotes	frame
T7934	701-707	NNS	denotes	clones
T7941	708-712	VBD	denotes	were
T7942	713-726	RB	denotes	independently
T7940	727-735	VBN	denotes	isolated
T7943	736-740	IN	denotes	from
T7944	741-744	DT	denotes	the
T7946	745-754	JJ	denotes	embryonic
T7947	755-758	CC	denotes	and
T7948	759-764	JJ	denotes	adult
T7949	765-770	NN	denotes	brain
T7945	771-780	NNS	denotes	libraries
T7950	780-782	,	denotes	,
T7951	782-794	RB	denotes	respectively
T7952	794-796	,	denotes	,
T7953	796-799	CC	denotes	and
T7954	800-803	DT	denotes	the
T7955	804-816	NNS	denotes	interactions
T7957	817-821	VBD	denotes	were
T7956	822-831	VBN	denotes	validated
T7958	831-832	.	denotes	.
T7959	832-864	sentence	denotes	The three clones encoded Cox11.
T7960	833-836	DT	denotes	The
T7962	837-842	CD	denotes	three
T7961	843-849	NNS	denotes	clones
T7963	850-857	VBD	denotes	encoded
T7964	858-863	NN	denotes	Cox11
T7965	863-864	.	denotes	.
T7966	864-1046	sentence	denotes	Interactions between Cox11, LD, and subdomains thereof were quantified by liquid β-galactosidase (Applied Biosystems, Foster City, California, United States) and growth assays [63].
T7967	865-877	NNS	denotes	Interactions
T7969	878-885	IN	denotes	between
T7970	886-891	NN	denotes	Cox11
T7971	891-893	,	denotes	,
T7972	893-895	NN	denotes	LD
T7973	895-897	,	denotes	,
T7974	897-900	CC	denotes	and
T7975	901-911	NNS	denotes	subdomains
T7976	912-919	RB	denotes	thereof
T7977	920-924	VBD	denotes	were
T7968	925-935	VBN	denotes	quantified
T7978	936-938	IN	denotes	by
T7979	939-945	JJ	denotes	liquid
T7981	946-947	NN	denotes	β
T7982	947-948	HYPH	denotes	-
T7980	948-961	NN	denotes	galactosidase
T7983	962-963	-LRB-	denotes	(
T7985	963-970	NNP	denotes	Applied
T7984	971-981	NNP	denotes	Biosystems
T7986	981-983	,	denotes	,
T7987	983-989	NNP	denotes	Foster
T7988	990-994	NNP	denotes	City
T7989	994-996	,	denotes	,
T7990	996-1006	NNP	denotes	California
T7991	1006-1008	,	denotes	,
T7992	1008-1014	NNP	denotes	United
T7993	1015-1021	NNP	denotes	States
T7994	1021-1022	-RRB-	denotes	)
T7995	1023-1026	CC	denotes	and
T7996	1027-1033	NN	denotes	growth
T7997	1034-1040	NNS	denotes	assays
T7998	1041-1042	-LRB-	denotes	[
T7999	1042-1044	CD	denotes	63
T8000	1044-1045	-RRB-	denotes	]
T8001	1045-1046	.	denotes	.
T8002	1046-1260	sentence	denotes	The maximum specific growth speed (μmax) was determined by calculating μmax = (ln(x t) − ln(x 0))/t, where x t is the OD600 of the culture at t = t, x 0 is the OD600 at t = 0 and t is the time between x 0 and x t.
T8003	1047-1050	DT	denotes	The
T8005	1051-1058	NN	denotes	maximum
T8006	1059-1067	JJ	denotes	specific
T8007	1068-1074	NN	denotes	growth
T8004	1075-1080	NN	denotes	speed
T8009	1081-1082	-LRB-	denotes	(
T8010	1082-1086	NN	denotes	μmax
T8011	1086-1087	-RRB-	denotes	)
T8012	1088-1091	VBD	denotes	was
T8008	1092-1102	VBN	denotes	determined
T8013	1103-1105	IN	denotes	by
T8014	1106-1117	VBG	denotes	calculating
T8015	1118-1122	NN	denotes	μmax
T8017	1123-1124	SYM	denotes	=
T8018	1125-1126	-LRB-	denotes	(
T8019	1126-1128	NN	denotes	ln
T8020	1128-1129	-LRB-	denotes	(
T8016	1129-1132	NN	denotes	x t
T8021	1132-1133	-RRB-	denotes	)
T8022	1134-1135	SYM	denotes	−
T8023	1136-1138	NN	denotes	ln
T8025	1138-1139	-LRB-	denotes	(
T8024	1139-1142	NN	denotes	x 0
T8026	1142-1143	-RRB-	denotes	)
T8027	1143-1144	-RRB-	denotes	)
T8028	1144-1145	SYM	denotes	/
T8029	1145-1146	NN	denotes	t
T8030	1146-1148	,	denotes	,
T8031	1148-1153	WRB	denotes	where
T8033	1154-1157	NN	denotes	x t
T8032	1158-1160	VBZ	denotes	is
T8034	1161-1164	DT	denotes	the
T8035	1165-1170	NN	denotes	OD600
T8036	1171-1173	IN	denotes	of
T8037	1174-1177	DT	denotes	the
T8038	1178-1185	NN	denotes	culture
T8039	1186-1188	IN	denotes	at
T8040	1189-1190	NN	denotes	t
T8042	1191-1192	SYM	denotes	=
T8041	1193-1194	NN	denotes	t
T8043	1194-1196	,	denotes	,
T8044	1196-1199	NN	denotes	x 0
T8045	1200-1202	VBZ	denotes	is
T8046	1203-1206	DT	denotes	the
T8047	1207-1212	NN	denotes	OD600
T8048	1213-1215	IN	denotes	at
T8049	1216-1217	NN	denotes	t
T8051	1218-1219	SYM	denotes	=
T8050	1220-1221	CD	denotes	0
T8052	1222-1225	CC	denotes	and
T8053	1226-1227	NN	denotes	t
T8054	1228-1230	VBZ	denotes	is
T8055	1231-1234	DT	denotes	the
T8056	1235-1239	NN	denotes	time
T8057	1240-1247	IN	denotes	between
T8058	1248-1251	NN	denotes	x 0
T8059	1252-1255	CC	denotes	and
T8060	1256-1259	NN	denotes	x t
T8061	1259-1260	.	denotes	.
T8062	1260-1412	sentence	denotes	Assays were performed with three independent clones and three samples of each clone, the results were averaged, and the standard deviations calculated.
T8063	1261-1267	NNS	denotes	Assays
T8065	1268-1272	VBD	denotes	were
T8064	1273-1282	VBN	denotes	performed
T8066	1283-1287	IN	denotes	with
T8067	1288-1293	CD	denotes	three
T8069	1294-1305	JJ	denotes	independent
T8068	1306-1312	NNS	denotes	clones
T8070	1313-1316	CC	denotes	and
T8071	1317-1322	CD	denotes	three
T8072	1323-1330	NNS	denotes	samples
T8073	1331-1333	IN	denotes	of
T8074	1334-1338	DT	denotes	each
T8075	1339-1344	NN	denotes	clone
T8076	1344-1346	,	denotes	,
T8077	1346-1349	DT	denotes	the
T8078	1350-1357	NNS	denotes	results
T8080	1358-1362	VBD	denotes	were
T8079	1363-1371	VBN	denotes	averaged
T8081	1371-1373	,	denotes	,
T8082	1373-1376	CC	denotes	and
T8083	1377-1380	DT	denotes	the
T8085	1381-1389	JJ	denotes	standard
T8084	1390-1400	NNS	denotes	deviations
T8086	1401-1411	VBN	denotes	calculated
T8087	1411-1412	.	denotes	.
T8119	1414-1418	NN	denotes	Site
T8121	1418-1419	HYPH	denotes	-
T8120	1419-1427	VBN	denotes	directed
T8123	1428-1431	CC	denotes	and
T8124	1432-1440	NN	denotes	deletion
T8122	1441-1452	NN	denotes	mutagenesis
T8125	1453-1456	CC	denotes	and
T8126	1457-1464	NN	denotes	plasmid
T8127	1465-1477	NN	denotes	construction
T8128	1477-1478	.	denotes	.
T8129	1478-1598	sentence	denotes	Deletion mutagenesis was carried out with pairs of primers against domains of interest described in the figure legends.
T8130	1479-1487	NN	denotes	Deletion
T8131	1488-1499	NN	denotes	mutagenesis
T8133	1500-1503	VBD	denotes	was
T8132	1504-1511	VBN	denotes	carried
T8134	1512-1515	RP	denotes	out
T8135	1516-1520	IN	denotes	with
T8136	1521-1526	NNS	denotes	pairs
T8137	1527-1529	IN	denotes	of
T8138	1530-1537	NNS	denotes	primers
T8139	1538-1545	IN	denotes	against
T8140	1546-1553	NNS	denotes	domains
T8141	1554-1556	IN	denotes	of
T8142	1557-1565	NN	denotes	interest
T8143	1566-1575	VBN	denotes	described
T8144	1576-1578	IN	denotes	in
T8145	1579-1582	DT	denotes	the
T8147	1583-1589	NN	denotes	figure
T8146	1590-1597	NNS	denotes	legends
T8148	1597-1598	.	denotes	.
T8149	1598-1708	sentence	denotes	PCR products were subcloned into pGEX-KG [64], HybriZAP pBD-GAL4 vector (Stratagene), and pBUTE [62] vectors.
T8150	1599-1602	NN	denotes	PCR
T8151	1603-1611	NNS	denotes	products
T8153	1612-1616	VBD	denotes	were
T8152	1617-1626	VBN	denotes	subcloned
T8154	1627-1631	IN	denotes	into
T8155	1632-1636	NN	denotes	pGEX
T8157	1636-1637	HYPH	denotes	-
T8156	1637-1639	NN	denotes	KG
T8158	1640-1641	-LRB-	denotes	[
T8159	1641-1643	CD	denotes	64
T8160	1643-1644	-RRB-	denotes	]
T8161	1644-1646	,	denotes	,
T8162	1646-1654	NN	denotes	HybriZAP
T8164	1655-1658	NN	denotes	pBD
T8166	1658-1659	HYPH	denotes	-
T8165	1659-1663	NN	denotes	GAL4
T8163	1664-1670	NN	denotes	vector
T8167	1671-1672	-LRB-	denotes	(
T8168	1672-1682	NNP	denotes	Stratagene
T8169	1682-1683	-RRB-	denotes	)
T8170	1683-1685	,	denotes	,
T8171	1685-1688	CC	denotes	and
T8172	1689-1694	NN	denotes	pBUTE
T8174	1695-1696	-LRB-	denotes	[
T8175	1696-1698	CD	denotes	62
T8176	1698-1699	-RRB-	denotes	]
T8173	1700-1707	NNS	denotes	vectors
T8177	1707-1708	.	denotes	.
T8178	1708-1768	sentence	denotes	GST-LDZIP alone comprised residues 447–483 of human RanBP2.
T8179	1709-1712	NN	denotes	GST
T8181	1712-1713	HYPH	denotes	-
T8180	1713-1718	NN	denotes	LDZIP
T8183	1719-1724	RB	denotes	alone
T8182	1725-1734	VBN	denotes	comprised
T8184	1735-1743	NNS	denotes	residues
T8185	1744-1747	CD	denotes	447
T8186	1747-1748	SYM	denotes	–
T8187	1748-1751	CD	denotes	483
T8188	1752-1754	IN	denotes	of
T8189	1755-1760	JJ	denotes	human
T8190	1761-1767	NN	denotes	RanBP2
T8191	1767-1768	.	denotes	.
T8257	1770-1773	NN	denotes	GST
T8259	1774-1778	VB	denotes	pull
T8260	1778-1779	HYPH	denotes	-
T8261	1779-1783	RP	denotes	down
T8262	1784-1787	CC	denotes	and
T8263	1788-1807	NN	denotes	immunoprecipitation
T8258	1808-1814	NNS	denotes	assays
T8264	1814-1815	.	denotes	.
T8265	1815-1948	sentence	denotes	CHAPS-solubilized retinal extracts, expression, and purification of GST-fused constructs were prepared as previously described [65].
T8266	1816-1821	NN	denotes	CHAPS
T8268	1821-1822	HYPH	denotes	-
T8267	1822-1833	VBN	denotes	solubilized
T8270	1834-1841	JJ	denotes	retinal
T8269	1842-1850	NNS	denotes	extracts
T8272	1850-1852	,	denotes	,
T8273	1852-1862	NN	denotes	expression
T8274	1862-1864	,	denotes	,
T8275	1864-1867	CC	denotes	and
T8276	1868-1880	NN	denotes	purification
T8277	1881-1883	IN	denotes	of
T8278	1884-1887	NN	denotes	GST
T8280	1887-1888	HYPH	denotes	-
T8279	1888-1893	VBN	denotes	fused
T8281	1894-1904	NNS	denotes	constructs
T8282	1905-1909	VBD	denotes	were
T8271	1910-1918	VBN	denotes	prepared
T8283	1919-1921	IN	denotes	as
T8285	1922-1932	RB	denotes	previously
T8284	1933-1942	VBN	denotes	described
T8286	1943-1944	-LRB-	denotes	[
T8287	1944-1946	CD	denotes	65
T8288	1946-1947	-RRB-	denotes	]
T8289	1947-1948	.	denotes	.
T8290	1948-2030	sentence	denotes	GST pull-down assays were carried out with 0.5–2.2 μM of GST-fused proteins [65].
T8291	1949-1952	NN	denotes	GST
T8293	1953-1957	VB	denotes	pull
T8294	1957-1958	HYPH	denotes	-
T8295	1958-1962	RP	denotes	down
T8292	1963-1969	NNS	denotes	assays
T8297	1970-1974	VBD	denotes	were
T8296	1975-1982	VBN	denotes	carried
T8298	1983-1986	RP	denotes	out
T8299	1987-1991	IN	denotes	with
T8300	1992-1995	CD	denotes	0.5
T8302	1995-1996	SYM	denotes	–
T8301	1996-1999	CD	denotes	2.2
T8303	2000-2002	NNS	denotes	μM
T8304	2003-2005	IN	denotes	of
T8305	2006-2009	NN	denotes	GST
T8307	2009-2010	HYPH	denotes	-
T8306	2010-2015	VBN	denotes	fused
T8308	2016-2024	NN	denotes	proteins
T8309	2025-2026	-LRB-	denotes	[
T8310	2026-2028	CD	denotes	65
T8311	2028-2029	-RRB-	denotes	]
T8312	2029-2030	.	denotes	.
T8313	2030-2151	sentence	denotes	Co-precipitates were resolved on SDS-PAGE and analyzed by Western blot with antibodies described in the Results section.
T8314	2031-2046	NNS	denotes	Co-precipitates
T8316	2047-2051	VBD	denotes	were
T8315	2052-2060	VBN	denotes	resolved
T8317	2061-2063	IN	denotes	on
T8318	2064-2067	NN	denotes	SDS
T8320	2067-2068	HYPH	denotes	-
T8319	2068-2072	NN	denotes	PAGE
T8321	2073-2076	CC	denotes	and
T8322	2077-2085	VBN	denotes	analyzed
T8323	2086-2088	IN	denotes	by
T8324	2089-2096	NNP	denotes	Western
T8325	2097-2101	NN	denotes	blot
T8326	2102-2106	IN	denotes	with
T8327	2107-2117	NNS	denotes	antibodies
T8328	2118-2127	VBN	denotes	described
T8329	2128-2130	IN	denotes	in
T8330	2131-2134	DT	denotes	the
T8332	2135-2142	NNS	denotes	Results
T8331	2143-2150	NN	denotes	section
T8333	2150-2151	.	denotes	.
T8334	2151-2323	sentence	denotes	Unfolded and partially denatured Cox11 were generated by incubating recombinant and native Cox11 overnight with 5.6 and 7 M guanidine hydrochloride and urea, respectively.
T8335	2152-2160	VBN	denotes	Unfolded
T8337	2161-2164	CC	denotes	and
T8338	2165-2174	RB	denotes	partially
T8339	2175-2184	VBN	denotes	denatured
T8336	2185-2190	NN	denotes	Cox11
T8341	2191-2195	VBD	denotes	were
T8340	2196-2205	VBN	denotes	generated
T8342	2206-2208	IN	denotes	by
T8343	2209-2219	VBG	denotes	incubating
T8344	2220-2231	JJ	denotes	recombinant
T8346	2232-2235	CC	denotes	and
T8347	2236-2242	JJ	denotes	native
T8345	2243-2248	NN	denotes	Cox11
T8348	2249-2258	RB	denotes	overnight
T8349	2259-2263	IN	denotes	with
T8350	2264-2267	CD	denotes	5.6
T8352	2268-2271	CC	denotes	and
T8353	2272-2273	CD	denotes	7
T8351	2274-2275	NNS	denotes	M
T8354	2276-2285	NN	denotes	guanidine
T8355	2286-2299	NN	denotes	hydrochloride
T8356	2300-2303	CC	denotes	and
T8357	2304-2308	NN	denotes	urea
T8358	2308-2310	,	denotes	,
T8359	2310-2322	RB	denotes	respectively
T8360	2322-2323	.	denotes	.
T8361	2323-2414	sentence	denotes	The Cox11 conformers were then diluted ~20-fold in CHAPS-binding buffer containing GST-LD.
T8362	2324-2327	DT	denotes	The
T8364	2328-2333	NN	denotes	Cox11
T8363	2334-2344	NNS	denotes	conformers
T8366	2345-2349	VBD	denotes	were
T8367	2350-2354	RB	denotes	then
T8365	2355-2362	VBN	denotes	diluted
T8368	2363-2364	SYM	denotes	~
T8369	2364-2371	RB	denotes	20-fold
T8370	2372-2374	IN	denotes	in
T8371	2375-2380	NN	denotes	CHAPS
T8373	2380-2381	HYPH	denotes	-
T8372	2381-2388	NN	denotes	binding
T8374	2389-2395	NN	denotes	buffer
T8375	2396-2406	VBG	denotes	containing
T8376	2407-2410	NN	denotes	GST
T8378	2410-2411	HYPH	denotes	-
T8377	2411-2413	NN	denotes	LD
T8379	2413-2414	.	denotes	.
T8380	2414-2564	sentence	denotes	Immunoprecipitation assays with 5 μg of antibody and Western blots (~ 200–400 ng/ml of antibody) were performed exactly as described previously [13].
T8381	2415-2434	NN	denotes	Immunoprecipitation
T8382	2435-2441	NNS	denotes	assays
T8384	2442-2446	IN	denotes	with
T8385	2447-2448	CD	denotes	5
T8386	2449-2451	NNS	denotes	μg
T8387	2452-2454	IN	denotes	of
T8388	2455-2463	NN	denotes	antibody
T8389	2464-2467	CC	denotes	and
T8390	2468-2475	NNP	denotes	Western
T8391	2476-2481	NNS	denotes	blots
T8392	2482-2483	-LRB-	denotes	(
T8394	2483-2484	SYM	denotes	~
T8396	2485-2488	CD	denotes	200
T8397	2488-2489	SYM	denotes	–
T8395	2489-2492	CD	denotes	400
T8393	2493-2495	NN	denotes	ng
T8398	2495-2496	SYM	denotes	/
T8399	2496-2498	NN	denotes	ml
T8400	2499-2501	IN	denotes	of
T8401	2502-2510	NN	denotes	antibody
T8402	2510-2511	-RRB-	denotes	)
T8403	2512-2516	VBD	denotes	were
T8383	2517-2526	VBN	denotes	performed
T8404	2527-2534	RB	denotes	exactly
T8406	2535-2537	IN	denotes	as
T8405	2538-2547	VBN	denotes	described
T8407	2548-2558	RB	denotes	previously
T8408	2559-2560	-LRB-	denotes	[
T8409	2560-2562	CD	denotes	13
T8410	2562-2563	-RRB-	denotes	]
T8411	2563-2564	.	denotes	.
T8483	2566-2576	NN	denotes	Hexokinase
T8485	2577-2578	CD	denotes	I
T8484	2579-2584	NN	denotes	assay
T8486	2584-2585	.	denotes	.
T8487	2585-2832	sentence	denotes	Hexokinase I activity was determined spectrophotometrically at 25 °C by the method of coupling the glucose-6-phosphate production via glucose-6-phosphate dehydrogenase with the change in the absorbance of NADPH at 340 nm and as described by [66].
T8488	2586-2596	NN	denotes	Hexokinase
T8490	2597-2598	CD	denotes	I
T8489	2599-2607	NN	denotes	activity
T8492	2608-2611	VBD	denotes	was
T8491	2612-2622	VBN	denotes	determined
T8493	2623-2645	RB	denotes	spectrophotometrically
T8494	2646-2648	IN	denotes	at
T8495	2649-2651	CD	denotes	25
T8496	2652-2654	NN	denotes	°C
T8497	2655-2657	IN	denotes	by
T8498	2658-2661	DT	denotes	the
T8499	2662-2668	NN	denotes	method
T8500	2669-2671	IN	denotes	of
T8501	2672-2680	VBG	denotes	coupling
T8502	2681-2684	DT	denotes	the
T8504	2685-2692	NN	denotes	glucose
T8506	2692-2693	HYPH	denotes	-
T8507	2693-2694	CD	denotes	6
T8508	2694-2695	HYPH	denotes	-
T8505	2695-2704	NN	denotes	phosphate
T8503	2705-2715	NN	denotes	production
T8509	2716-2719	IN	denotes	via
T8510	2720-2727	NN	denotes	glucose
T8512	2727-2728	HYPH	denotes	-
T8513	2728-2729	CD	denotes	6
T8514	2729-2730	HYPH	denotes	-
T8511	2730-2739	NN	denotes	phosphate
T8515	2740-2753	NN	denotes	dehydrogenase
T8516	2754-2758	IN	denotes	with
T8517	2759-2762	DT	denotes	the
T8518	2763-2769	NN	denotes	change
T8519	2770-2772	IN	denotes	in
T8520	2773-2776	DT	denotes	the
T8521	2777-2787	NN	denotes	absorbance
T8522	2788-2790	IN	denotes	of
T8523	2791-2796	NN	denotes	NADPH
T8524	2797-2799	IN	denotes	at
T8525	2800-2803	CD	denotes	340
T8526	2804-2806	NN	denotes	nm
T8527	2807-2810	CC	denotes	and
T8528	2811-2813	IN	denotes	as
T8529	2814-2823	VBN	denotes	described
T8530	2824-2826	IN	denotes	by
T8531	2827-2828	-LRB-	denotes	[
T8532	2828-2830	CD	denotes	66
T8533	2830-2831	-RRB-	denotes	]
T8534	2831-2832	.	denotes	.
T8535	2832-3118	sentence	denotes	The reaction was started by the addition of purified brain hexokinase I (0.24 μg) (gift from J. Wilson) to 1.0 ml of reaction mixture containing 0.05 M Tris-HCl (pH 8.5), 7.4 mM MgCl2, 6.6 mM ATP, 0.65 mM NADP, 11.1 mM monothioglycerol, and 1 unit of glucose 6-phosphate dehydrogenase.
T8536	2833-2836	DT	denotes	The
T8537	2837-2845	NN	denotes	reaction
T8539	2846-2849	VBD	denotes	was
T8538	2850-2857	VBN	denotes	started
T8540	2858-2860	IN	denotes	by
T8541	2861-2864	DT	denotes	the
T8542	2865-2873	NN	denotes	addition
T8543	2874-2876	IN	denotes	of
T8544	2877-2885	VBN	denotes	purified
T8546	2886-2891	NN	denotes	brain
T8545	2892-2902	NN	denotes	hexokinase
T8547	2903-2904	CD	denotes	I
T8548	2905-2906	-LRB-	denotes	(
T8550	2906-2910	CD	denotes	0.24
T8549	2911-2913	NNS	denotes	μg
T8551	2913-2914	-RRB-	denotes	)
T8552	2915-2916	-LRB-	denotes	(
T8553	2916-2920	NN	denotes	gift
T8554	2921-2925	IN	denotes	from
T8555	2926-2928	NNP	denotes	J.
T8556	2929-2935	NNP	denotes	Wilson
T8557	2935-2936	-RRB-	denotes	)
T8558	2937-2939	IN	denotes	to
T8559	2940-2943	CD	denotes	1.0
T8560	2944-2946	NN	denotes	ml
T8561	2947-2949	IN	denotes	of
T8562	2950-2958	NN	denotes	reaction
T8563	2959-2966	NN	denotes	mixture
T8564	2967-2977	VBG	denotes	containing
T8565	2978-2982	CD	denotes	0.05
T8566	2983-2984	NNS	denotes	M
T8568	2985-2989	NN	denotes	Tris
T8569	2989-2990	HYPH	denotes	-
T8567	2990-2993	NN	denotes	HCl
T8570	2994-2995	-LRB-	denotes	(
T8571	2995-2997	NN	denotes	pH
T8572	2998-3001	CD	denotes	8.5
T8573	3001-3002	-RRB-	denotes	)
T8574	3002-3004	,	denotes	,
T8575	3004-3007	CD	denotes	7.4
T8576	3008-3010	NNS	denotes	mM
T8577	3011-3016	NN	denotes	MgCl2
T8578	3016-3018	,	denotes	,
T8579	3018-3021	CD	denotes	6.6
T8580	3022-3024	NNS	denotes	mM
T8581	3025-3028	NN	denotes	ATP
T8582	3028-3030	,	denotes	,
T8583	3030-3034	CD	denotes	0.65
T8584	3035-3037	NNS	denotes	mM
T8585	3038-3042	NN	denotes	NADP
T8586	3042-3044	,	denotes	,
T8587	3044-3048	CD	denotes	11.1
T8588	3049-3051	NNS	denotes	mM
T8589	3052-3068	NN	denotes	monothioglycerol
T8590	3068-3070	,	denotes	,
T8591	3070-3073	CC	denotes	and
T8592	3074-3075	CD	denotes	1
T8593	3076-3080	NN	denotes	unit
T8594	3081-3083	IN	denotes	of
T8595	3084-3091	NN	denotes	glucose
T8597	3092-3093	CD	denotes	6
T8598	3093-3094	HYPH	denotes	-
T8596	3094-3103	NN	denotes	phosphate
T8599	3104-3117	NN	denotes	dehydrogenase
T8600	3117-3118	.	denotes	.
T8601	3118-3311	sentence	denotes	In the case of hexokinase activity measured in the presence of Cox11 and LD, the purified HKI was incubated with the recombinant proteins for 15 min at 4 °C before measurement of the activity.
T8602	3119-3121	IN	denotes	In
T8604	3122-3125	DT	denotes	the
T8605	3126-3130	NN	denotes	case
T8606	3131-3133	IN	denotes	of
T8607	3134-3144	NN	denotes	hexokinase
T8608	3145-3153	NN	denotes	activity
T8609	3154-3162	VBN	denotes	measured
T8610	3163-3165	IN	denotes	in
T8611	3166-3169	DT	denotes	the
T8612	3170-3178	NN	denotes	presence
T8613	3179-3181	IN	denotes	of
T8614	3182-3187	NN	denotes	Cox11
T8615	3188-3191	CC	denotes	and
T8616	3192-3194	NN	denotes	LD
T8617	3194-3196	,	denotes	,
T8618	3196-3199	DT	denotes	the
T8620	3200-3208	VBN	denotes	purified
T8619	3209-3212	NN	denotes	HKI
T8621	3213-3216	VBD	denotes	was
T8603	3217-3226	VBN	denotes	incubated
T8622	3227-3231	IN	denotes	with
T8623	3232-3235	DT	denotes	the
T8625	3236-3247	JJ	denotes	recombinant
T8624	3248-3256	NN	denotes	proteins
T8626	3257-3260	IN	denotes	for
T8627	3261-3263	CD	denotes	15
T8628	3264-3267	NNS	denotes	min
T8629	3268-3270	IN	denotes	at
T8630	3271-3272	CD	denotes	4
T8631	3273-3275	NN	denotes	°C
T8632	3276-3282	IN	denotes	before
T8633	3283-3294	NN	denotes	measurement
T8634	3295-3297	IN	denotes	of
T8635	3298-3301	DT	denotes	the
T8636	3302-3310	NN	denotes	activity
T8637	3310-3311	.	denotes	.
T8638	3311-3408	sentence	denotes	The data were fitted directly into the Michaelis-Menten equation using SIGMAPLOT (SPSS Science).
T8639	3312-3315	DT	denotes	The
T8640	3316-3320	NNS	denotes	data
T8642	3321-3325	VBD	denotes	were
T8641	3326-3332	VBN	denotes	fitted
T8643	3333-3341	RB	denotes	directly
T8644	3342-3346	IN	denotes	into
T8645	3347-3350	DT	denotes	the
T8647	3351-3360	NNP	denotes	Michaelis
T8649	3360-3361	HYPH	denotes	-
T8648	3361-3367	NNP	denotes	Menten
T8646	3368-3376	NN	denotes	equation
T8650	3377-3382	VBG	denotes	using
T8651	3383-3392	NNP	denotes	SIGMAPLOT
T8652	3393-3394	-LRB-	denotes	(
T8654	3394-3398	NNP	denotes	SPSS
T8653	3399-3406	NNP	denotes	Science
T8655	3406-3407	-RRB-	denotes	)
T8656	3407-3408	.	denotes	.
T8700	3410-3420	NNS	denotes	Antibodies
T8701	3420-3421	.	denotes	.
T8702	3421-3601	sentence	denotes	Rabbit antisera were raised against the recombinant murine Cox11 (residues 40–275) and affinity-purified under non-denaturing conditions (Stereogene) as previously described [13].
T8703	3422-3428	NN	denotes	Rabbit
T8704	3429-3437	NNS	denotes	antisera
T8706	3438-3442	VBD	denotes	were
T8705	3443-3449	VBN	denotes	raised
T8707	3450-3457	IN	denotes	against
T8708	3458-3461	DT	denotes	the
T8710	3462-3473	JJ	denotes	recombinant
T8711	3474-3480	JJ	denotes	murine
T8709	3481-3486	NN	denotes	Cox11
T8712	3487-3488	-LRB-	denotes	(
T8714	3488-3496	NNS	denotes	residues
T8713	3497-3499	CD	denotes	40
T8715	3499-3500	SYM	denotes	–
T8716	3500-3503	CD	denotes	275
T8717	3503-3504	-RRB-	denotes	)
T8718	3505-3508	CC	denotes	and
T8719	3509-3517	NN	denotes	affinity
T8721	3517-3518	HYPH	denotes	-
T8720	3518-3526	VBN	denotes	purified
T8722	3527-3532	IN	denotes	under
T8723	3533-3547	NN	denotes	non-denaturing
T8724	3548-3558	NNS	denotes	conditions
T8725	3559-3560	-LRB-	denotes	(
T8726	3560-3570	NNP	denotes	Stereogene
T8727	3570-3571	-RRB-	denotes	)
T8728	3572-3574	IN	denotes	as
T8730	3575-3585	RB	denotes	previously
T8729	3586-3595	VBN	denotes	described
T8731	3596-3597	-LRB-	denotes	[
T8732	3597-3599	CD	denotes	13
T8733	3599-3600	-RRB-	denotes	]
T8734	3600-3601	.	denotes	.
T8735	3601-3663	sentence	denotes	Cox11 antibodies were used at 200 ng/ml for Western analysis.
T8736	3602-3607	NN	denotes	Cox11
T8737	3608-3618	NNS	denotes	antibodies
T8739	3619-3623	VBD	denotes	were
T8738	3624-3628	VBN	denotes	used
T8740	3629-3631	IN	denotes	at
T8741	3632-3635	CD	denotes	200
T8742	3636-3638	NNS	denotes	ng
T8743	3638-3639	SYM	denotes	/
T8744	3639-3641	NN	denotes	ml
T8745	3642-3645	IN	denotes	for
T8746	3646-3653	NNP	denotes	Western
T8747	3654-3662	NN	denotes	analysis
T8748	3662-3663	.	denotes	.
T8749	3663-3849	sentence	denotes	The monoclonal antibody Hsp70 was from Affinity Bioreagents (Golden, Colorado, United States) and antibodies against the KBD (JX2) and ZnF domains of RanBP2 have been described [13,16].
T8750	3664-3667	DT	denotes	The
T8752	3668-3678	JJ	denotes	monoclonal
T8753	3679-3687	NN	denotes	antibody
T8751	3688-3693	NN	denotes	Hsp70
T8754	3694-3697	VBD	denotes	was
T8755	3698-3702	IN	denotes	from
T8756	3703-3711	NNP	denotes	Affinity
T8757	3712-3723	NNPS	denotes	Bioreagents
T8758	3724-3725	-LRB-	denotes	(
T8759	3725-3731	NNP	denotes	Golden
T8760	3731-3733	,	denotes	,
T8761	3733-3741	NNP	denotes	Colorado
T8762	3741-3743	,	denotes	,
T8763	3743-3749	NNP	denotes	United
T8764	3750-3756	NNP	denotes	States
T8765	3756-3757	-RRB-	denotes	)
T8766	3758-3761	CC	denotes	and
T8767	3762-3772	NNS	denotes	antibodies
T8769	3773-3780	IN	denotes	against
T8770	3781-3784	DT	denotes	the
T8772	3785-3788	NN	denotes	KBD
T8773	3789-3790	-LRB-	denotes	(
T8774	3790-3793	NN	denotes	JX2
T8775	3793-3794	-RRB-	denotes	)
T8776	3795-3798	CC	denotes	and
T8777	3799-3802	NN	denotes	ZnF
T8771	3803-3810	NNS	denotes	domains
T8778	3811-3813	IN	denotes	of
T8779	3814-3820	NN	denotes	RanBP2
T8780	3821-3825	VBP	denotes	have
T8781	3826-3830	VBN	denotes	been
T8768	3831-3840	VBN	denotes	described
T8782	3841-3842	-LRB-	denotes	[
T8784	3842-3844	CD	denotes	13
T8785	3844-3845	,	denotes	,
T8783	3845-3847	CD	denotes	16
T8786	3847-3848	-RRB-	denotes	]
T8787	3848-3849	.	denotes	.
T8788	3849-3935	sentence	denotes	Polyclonal and monoclonal antibodies against Hexokinase I were provided by J. Wilson.
T8789	3850-3860	JJ	denotes	Polyclonal
T8791	3861-3864	CC	denotes	and
T8792	3865-3875	JJ	denotes	monoclonal
T8790	3876-3886	NNS	denotes	antibodies
T8794	3887-3894	IN	denotes	against
T8795	3895-3905	NN	denotes	Hexokinase
T8796	3906-3907	PRP	denotes	I
T8797	3908-3912	VBD	denotes	were
T8793	3913-3921	VBN	denotes	provided
T8798	3922-3924	IN	denotes	by
T8799	3925-3927	NNP	denotes	J.
T8800	3928-3934	NNP	denotes	Wilson
T8801	3934-3935	.	denotes	.
T8802	3935-4014	sentence	denotes	The mAb414 was purchased from Abcam (Cambridge, Massachusetts, United States).
T8803	3936-3939	DT	denotes	The
T8804	3940-3946	NN	denotes	mAb414
T8806	3947-3950	VBD	denotes	was
T8805	3951-3960	VBN	denotes	purchased
T8807	3961-3965	IN	denotes	from
T8808	3966-3971	NNP	denotes	Abcam
T8809	3972-3973	-LRB-	denotes	(
T8810	3973-3982	NNP	denotes	Cambridge
T8811	3982-3984	,	denotes	,
T8812	3984-3997	NNP	denotes	Massachusetts
T8813	3997-3999	,	denotes	,
T8814	3999-4005	NNP	denotes	United
T8815	4006-4012	NNP	denotes	States
T8816	4012-4013	-RRB-	denotes	)
T8817	4013-4014	.	denotes	.
T8883	4016-4035	NN	denotes	Immunocytochemistry
T8884	4036-4039	CC	denotes	and
T8885	4040-4050	NN	denotes	microscopy
T8886	4050-4051	.	denotes	.
T8887	4051-4182	sentence	denotes	Retina dissections, radial cryosections (~6 μm), and immunohistochemistry procedures were carried out as described elsewhere [19].
T8888	4052-4058	NN	denotes	Retina
T8889	4059-4070	NNS	denotes	dissections
T8891	4070-4072	,	denotes	,
T8892	4072-4078	JJ	denotes	radial
T8893	4079-4091	NNS	denotes	cryosections
T8894	4092-4093	-LRB-	denotes	(
T8896	4093-4094	SYM	denotes	~
T8897	4094-4095	CD	denotes	6
T8895	4096-4098	NNS	denotes	μm
T8898	4098-4099	-RRB-	denotes	)
T8899	4099-4101	,	denotes	,
T8900	4101-4104	CC	denotes	and
T8901	4105-4125	NN	denotes	immunohistochemistry
T8902	4126-4136	NNS	denotes	procedures
T8903	4137-4141	VBD	denotes	were
T8890	4142-4149	VBN	denotes	carried
T8904	4150-4153	RP	denotes	out
T8905	4154-4156	IN	denotes	as
T8906	4157-4166	VBN	denotes	described
T8907	4167-4176	RB	denotes	elsewhere
T8908	4177-4178	-LRB-	denotes	[
T8909	4178-4180	CD	denotes	19
T8910	4180-4181	-RRB-	denotes	]
T8911	4181-4182	.	denotes	.
T8912	4182-4327	sentence	denotes	Brains from 3- to 5-mo-old C57Bl/6 mice were fixed overnight in 2% paraformaldehyde, infused with 30% sucrose, and processed for cryosectioning.
T8913	4183-4189	NNS	denotes	Brains
T8915	4190-4194	IN	denotes	from
T8916	4195-4196	CD	denotes	3
T8918	4196-4197	HYPH	denotes	-
T8919	4198-4200	IN	denotes	to
T8917	4201-4202	CD	denotes	5
T8921	4202-4203	HYPH	denotes	-
T8920	4203-4205	NN	denotes	mo
T8923	4205-4206	HYPH	denotes	-
T8922	4206-4209	JJ	denotes	old
T8925	4210-4215	NN	denotes	C57Bl
T8926	4215-4216	HYPH	denotes	/
T8927	4216-4217	CD	denotes	6
T8924	4218-4222	NNS	denotes	mice
T8928	4223-4227	VBD	denotes	were
T8914	4228-4233	VBN	denotes	fixed
T8929	4234-4243	RB	denotes	overnight
T8930	4244-4246	IN	denotes	in
T8931	4247-4248	CD	denotes	2
T8932	4248-4249	NN	denotes	%
T8933	4250-4266	NN	denotes	paraformaldehyde
T8934	4266-4268	,	denotes	,
T8935	4268-4275	VBN	denotes	infused
T8936	4276-4280	IN	denotes	with
T8937	4281-4283	CD	denotes	30
T8938	4283-4284	NN	denotes	%
T8939	4285-4292	NN	denotes	sucrose
T8940	4292-4294	,	denotes	,
T8941	4294-4297	CC	denotes	and
T8942	4298-4307	VBN	denotes	processed
T8943	4308-4311	IN	denotes	for
T8944	4312-4326	VBG	denotes	cryosectioning
T8945	4326-4327	.	denotes	.
T8946	4327-4405	sentence	denotes	Primary brain neurons and glial cells were prepared from the cerebral cortex.
T8947	4328-4335	JJ	denotes	Primary
T8949	4336-4341	NN	denotes	brain
T8948	4342-4349	NNS	denotes	neurons
T8951	4350-4353	CC	denotes	and
T8952	4354-4359	JJ	denotes	glial
T8953	4360-4365	NNS	denotes	cells
T8954	4366-4370	VBD	denotes	were
T8950	4371-4379	VBN	denotes	prepared
T8955	4380-4384	IN	denotes	from
T8956	4385-4388	DT	denotes	the
T8958	4389-4397	JJ	denotes	cerebral
T8957	4398-4404	NN	denotes	cortex
T8959	4404-4405	.	denotes	.
T8960	4405-4477	sentence	denotes	This was macerated in the Hank's Balanced Salt solution and triturated.
T8961	4406-4410	DT	denotes	This
T8963	4411-4414	VBD	denotes	was
T8962	4415-4424	VBN	denotes	macerated
T8964	4425-4427	IN	denotes	in
T8965	4428-4431	DT	denotes	the
T8967	4432-4436	NNP	denotes	Hank
T8969	4436-4438	POS	denotes	's
T8970	4439-4447	NNP	denotes	Balanced
T8968	4448-4452	NNP	denotes	Salt
T8966	4453-4461	NN	denotes	solution
T8971	4462-4465	CC	denotes	and
T8972	4466-4476	VBN	denotes	triturated
T8973	4476-4477	.	denotes	.
T8974	4477-4714	sentence	denotes	Primary cells were cultured in DMEM (GIBCO, San Diego, California, United States) and collagen-coated 35-mm glass bottom culture dishes (MatTek Corporation, Ashland, Maine) at 5% CO2/37 °C for ~2 d and processed for immunocytochemistry.
T8975	4478-4485	JJ	denotes	Primary
T8976	4486-4491	NNS	denotes	cells
T8978	4492-4496	VBD	denotes	were
T8977	4497-4505	VBN	denotes	cultured
T8979	4506-4508	IN	denotes	in
T8980	4509-4513	NN	denotes	DMEM
T8981	4514-4515	-LRB-	denotes	(
T8982	4515-4520	NNP	denotes	GIBCO
T8983	4520-4522	,	denotes	,
T8984	4522-4525	NNP	denotes	San
T8985	4526-4531	NNP	denotes	Diego
T8986	4531-4533	,	denotes	,
T8987	4533-4543	NNP	denotes	California
T8988	4543-4545	,	denotes	,
T8989	4545-4551	NNP	denotes	United
T8990	4552-4558	NNP	denotes	States
T8991	4558-4559	-RRB-	denotes	)
T8992	4560-4563	CC	denotes	and
T8993	4564-4572	NN	denotes	collagen
T8995	4572-4573	HYPH	denotes	-
T8994	4573-4579	VBN	denotes	coated
T8997	4580-4582	CD	denotes	35
T8999	4582-4583	HYPH	denotes	-
T8998	4583-4585	NN	denotes	mm
T9000	4586-4591	NN	denotes	glass
T9001	4592-4598	NN	denotes	bottom
T9002	4599-4606	NN	denotes	culture
T8996	4607-4613	NNS	denotes	dishes
T9003	4614-4615	-LRB-	denotes	(
T9005	4615-4621	NNP	denotes	MatTek
T9004	4622-4633	NNP	denotes	Corporation
T9006	4633-4635	,	denotes	,
T9007	4635-4642	NNP	denotes	Ashland
T9008	4642-4644	,	denotes	,
T9009	4644-4649	NNP	denotes	Maine
T9010	4649-4650	-RRB-	denotes	)
T9011	4651-4653	IN	denotes	at
T9012	4654-4655	CD	denotes	5
T9013	4655-4656	NN	denotes	%
T9014	4657-4660	NN	denotes	CO2
T9015	4660-4661	HYPH	denotes	/
T9016	4661-4663	CD	denotes	37
T9017	4664-4666	NN	denotes	°C
T9018	4667-4670	IN	denotes	for
T9019	4671-4672	SYM	denotes	~
T9020	4672-4673	CD	denotes	2
T9021	4674-4675	NNS	denotes	d
T9022	4676-4679	CC	denotes	and
T9023	4680-4689	VBN	denotes	processed
T9024	4690-4693	IN	denotes	for
T9025	4694-4713	NN	denotes	immunocytochemistry
T9026	4713-4714	.	denotes	.
T9027	4714-4776	sentence	denotes	Primary antibodies were used at concentrations ~2.5–10 μg/ml.
T9028	4715-4722	JJ	denotes	Primary
T9029	4723-4733	NNS	denotes	antibodies
T9031	4734-4738	VBD	denotes	were
T9030	4739-4743	VBN	denotes	used
T9032	4744-4746	IN	denotes	at
T9033	4747-4761	NNS	denotes	concentrations
T9034	4762-4763	SYM	denotes	~
T9036	4763-4766	CD	denotes	2.5
T9037	4766-4767	SYM	denotes	–
T9035	4767-4769	CD	denotes	10
T9038	4770-4772	NN	denotes	μg
T9039	4772-4773	SYM	denotes	/
T9040	4773-4775	NN	denotes	ml
T9041	4775-4776	.	denotes	.
T9042	4776-4905	sentence	denotes	Alexa 488- and Alexa 594-conjugated secondary antibodies (2.5 μg/ml) (Molecular Probes) were used for visualization of proteins.
T9043	4777-4782	NNP	denotes	Alexa
T9045	4783-4786	CD	denotes	488
T9046	4786-4787	HYPH	denotes	-
T9047	4788-4791	CC	denotes	and
T9048	4792-4797	NNP	denotes	Alexa
T9049	4798-4801	JJ	denotes	594
T9050	4801-4802	HYPH	denotes	-
T9044	4802-4812	JJ	denotes	conjugated
T9052	4813-4822	JJ	denotes	secondary
T9051	4823-4833	NNS	denotes	antibodies
T9054	4834-4835	-LRB-	denotes	(
T9056	4835-4838	CD	denotes	2.5
T9055	4839-4841	NNS	denotes	μg
T9057	4841-4842	SYM	denotes	/
T9058	4842-4844	NN	denotes	ml
T9059	4844-4845	-RRB-	denotes	)
T9060	4846-4847	-LRB-	denotes	(
T9062	4847-4856	NNP	denotes	Molecular
T9061	4857-4863	NNP	denotes	Probes
T9063	4863-4864	-RRB-	denotes	)
T9064	4865-4869	VBD	denotes	were
T9053	4870-4874	VBN	denotes	used
T9065	4875-4878	IN	denotes	for
T9066	4879-4892	NN	denotes	visualization
T9067	4893-4895	IN	denotes	of
T9068	4896-4904	NN	denotes	proteins
T9069	4904-4905	.	denotes	.
T9070	4905-5000	sentence	denotes	Crossover of fluorescent probes, background, and autofluorescence were found to be negligible.
T9071	4906-4915	NN	denotes	Crossover
T9073	4916-4918	IN	denotes	of
T9074	4919-4930	JJ	denotes	fluorescent
T9075	4931-4937	NNS	denotes	probes
T9076	4937-4939	,	denotes	,
T9077	4939-4949	NN	denotes	background
T9078	4949-4951	,	denotes	,
T9079	4951-4954	CC	denotes	and
T9080	4955-4971	NN	denotes	autofluorescence
T9081	4972-4976	VBD	denotes	were
T9072	4977-4982	VBN	denotes	found
T9082	4983-4985	TO	denotes	to
T9083	4986-4988	VB	denotes	be
T9084	4989-4999	JJ	denotes	negligible
T9085	4999-5000	.	denotes	.
T9086	5000-5238	sentence	denotes	Visualization of specimens and localization of proteins were carried out by wide-field epifluorescence microscopy on Nikon (Tokyo, Japan) E600 upright and TE2000U inverted research microscopes equipped with similar Apochromat objectives.
T9087	5001-5014	NN	denotes	Visualization
T9089	5015-5017	IN	denotes	of
T9090	5018-5027	NNS	denotes	specimens
T9091	5028-5031	CC	denotes	and
T9092	5032-5044	NN	denotes	localization
T9093	5045-5047	IN	denotes	of
T9094	5048-5056	NN	denotes	proteins
T9095	5057-5061	VBD	denotes	were
T9088	5062-5069	VBN	denotes	carried
T9096	5070-5073	RP	denotes	out
T9097	5074-5076	IN	denotes	by
T9098	5077-5081	JJ	denotes	wide
T9100	5081-5082	HYPH	denotes	-
T9099	5082-5087	NN	denotes	field
T9102	5088-5103	NN	denotes	epifluorescence
T9101	5104-5114	NN	denotes	microscopy
T9103	5115-5117	IN	denotes	on
T9104	5118-5123	NNP	denotes	Nikon
T9106	5124-5125	-LRB-	denotes	(
T9107	5125-5130	NNP	denotes	Tokyo
T9108	5130-5132	,	denotes	,
T9109	5132-5137	NNP	denotes	Japan
T9110	5137-5138	-RRB-	denotes	)
T9111	5139-5143	NN	denotes	E600
T9105	5144-5151	NN	denotes	upright
T9112	5152-5155	CC	denotes	and
T9113	5156-5163	NN	denotes	TE2000U
T9115	5164-5172	VBN	denotes	inverted
T9116	5173-5181	NN	denotes	research
T9114	5182-5193	NNS	denotes	microscopes
T9117	5194-5202	VBN	denotes	equipped
T9118	5203-5207	IN	denotes	with
T9119	5208-5215	JJ	denotes	similar
T9121	5216-5226	NN	denotes	Apochromat
T9120	5227-5237	NNS	denotes	objectives
T9122	5237-5238	.	denotes	.
T9123	5238-5445	sentence	denotes	Images with the Nikon E600 were acquired with a SPOT-RT digital camera coupled to the microscope and driven by SPOT Imaging v4.0 software (Diagnostic Instruments, Sterling Heights, Michigan, United States).
T9124	5239-5245	NNS	denotes	Images
T9126	5246-5250	IN	denotes	with
T9127	5251-5254	DT	denotes	the
T9129	5255-5260	NNP	denotes	Nikon
T9128	5261-5265	NN	denotes	E600
T9130	5266-5270	VBD	denotes	were
T9125	5271-5279	VBN	denotes	acquired
T9131	5280-5284	IN	denotes	with
T9132	5285-5286	DT	denotes	a
T9134	5287-5291	NN	denotes	SPOT
T9136	5291-5292	HYPH	denotes	-
T9135	5292-5294	NN	denotes	RT
T9137	5295-5302	JJ	denotes	digital
T9133	5303-5309	NN	denotes	camera
T9138	5310-5317	VBN	denotes	coupled
T9139	5318-5320	IN	denotes	to
T9140	5321-5324	DT	denotes	the
T9141	5325-5335	NN	denotes	microscope
T9142	5336-5339	CC	denotes	and
T9143	5340-5346	VBN	denotes	driven
T9144	5347-5349	IN	denotes	by
T9145	5350-5354	NN	denotes	SPOT
T9147	5355-5362	NN	denotes	Imaging
T9146	5363-5367	NN	denotes	v4.0
T9148	5368-5376	NN	denotes	software
T9149	5377-5378	-LRB-	denotes	(
T9151	5378-5388	NNP	denotes	Diagnostic
T9150	5389-5400	NNPS	denotes	Instruments
T9152	5400-5402	,	denotes	,
T9153	5402-5410	NNP	denotes	Sterling
T9154	5411-5418	NNP	denotes	Heights
T9155	5418-5420	,	denotes	,
T9156	5420-5428	NNP	denotes	Michigan
T9157	5428-5430	,	denotes	,
T9158	5430-5436	NNP	denotes	United
T9159	5437-5443	NNP	denotes	States
T9160	5443-5444	-RRB-	denotes	)
T9161	5444-5445	.	denotes	.
T9162	5445-5557	sentence	denotes	All images were captured at nonsaturating integration levels, 12-bit mono black/white, and then pseudo-colored.
T9163	5446-5449	DT	denotes	All
T9164	5450-5456	NNS	denotes	images
T9166	5457-5461	VBD	denotes	were
T9165	5462-5470	VBN	denotes	captured
T9167	5471-5473	IN	denotes	at
T9168	5474-5487	NN	denotes	nonsaturating
T9170	5488-5499	NN	denotes	integration
T9169	5500-5506	NNS	denotes	levels
T9171	5506-5508	,	denotes	,
T9172	5508-5510	CD	denotes	12
T9174	5510-5511	HYPH	denotes	-
T9173	5511-5514	JJ	denotes	bit
T9176	5515-5519	NN	denotes	mono
T9177	5520-5525	JJ	denotes	black
T9178	5525-5526	HYPH	denotes	/
T9175	5526-5531	JJ	denotes	white
T9179	5531-5533	,	denotes	,
T9180	5533-5536	CC	denotes	and
T9181	5537-5541	RB	denotes	then
T9182	5542-5556	VBN	denotes	pseudo-colored
T9183	5556-5557	.	denotes	.
T9184	5557-5953	sentence	denotes	Protein colocalization analysis was carried out on the Nikon TE2000U microscope equipped with appropriate excitation and emission filter wheels, cube filters, 100 W mercury light source, Nomarski/DIC, and Plan Apochromat optics (100×, 60×, and 40× oil objectives with NA of 1.4, 40 ×LWD, and 20 ×LWD objectives and encoded motorized Z-Stage (Prior Scientific, Rockland, Maryland, United States).
T9185	5558-5565	NN	denotes	Protein
T9186	5566-5580	NN	denotes	colocalization
T9187	5581-5589	NN	denotes	analysis
T9189	5590-5593	VBD	denotes	was
T9188	5594-5601	VBN	denotes	carried
T9190	5602-5605	RP	denotes	out
T9191	5606-5608	IN	denotes	on
T9192	5609-5612	DT	denotes	the
T9194	5613-5618	NNP	denotes	Nikon
T9195	5619-5626	NN	denotes	TE2000U
T9193	5627-5637	NN	denotes	microscope
T9196	5638-5646	VBN	denotes	equipped
T9197	5647-5651	IN	denotes	with
T9198	5652-5663	JJ	denotes	appropriate
T9200	5664-5674	NN	denotes	excitation
T9201	5675-5678	CC	denotes	and
T9202	5679-5687	NN	denotes	emission
T9203	5688-5694	NN	denotes	filter
T9199	5695-5701	NNS	denotes	wheels
T9204	5701-5703	,	denotes	,
T9205	5703-5707	NN	denotes	cube
T9206	5708-5715	NNS	denotes	filters
T9207	5715-5717	,	denotes	,
T9208	5717-5720	CD	denotes	100
T9209	5721-5722	NNP	denotes	W
T9211	5723-5730	NN	denotes	mercury
T9212	5731-5736	NN	denotes	light
T9210	5737-5743	NN	denotes	source
T9213	5743-5745	,	denotes	,
T9214	5745-5753	NNP	denotes	Nomarski
T9216	5753-5754	HYPH	denotes	/
T9215	5754-5757	NNP	denotes	DIC
T9218	5757-5759	,	denotes	,
T9219	5759-5762	CC	denotes	and
T9220	5763-5767	NNP	denotes	Plan
T9221	5768-5778	NNP	denotes	Apochromat
T9217	5779-5785	NNS	denotes	optics
T9222	5786-5787	-LRB-	denotes	(
T9223	5787-5790	CD	denotes	100
T9225	5790-5791	SYM	denotes	×
T9226	5791-5793	,	denotes	,
T9227	5793-5795	CD	denotes	60
T9228	5795-5796	SYM	denotes	×
T9229	5796-5798	,	denotes	,
T9230	5798-5801	CC	denotes	and
T9231	5802-5804	CD	denotes	40
T9232	5804-5805	SYM	denotes	×
T9233	5806-5809	NN	denotes	oil
T9224	5810-5820	NNS	denotes	objectives
T9234	5821-5825	IN	denotes	with
T9235	5826-5828	NN	denotes	NA
T9236	5829-5831	IN	denotes	of
T9237	5832-5835	CD	denotes	1.4
T9238	5835-5837	,	denotes	,
T9239	5837-5839	CD	denotes	40
T9241	5840-5841	SYM	denotes	×
T9240	5841-5844	NN	denotes	LWD
T9243	5844-5846	,	denotes	,
T9244	5846-5849	CC	denotes	and
T9245	5850-5852	CD	denotes	20
T9247	5853-5854	SYM	denotes	×
T9246	5854-5857	NN	denotes	LWD
T9242	5858-5868	NNS	denotes	objectives
T9248	5869-5872	CC	denotes	and
T9249	5873-5880	VBN	denotes	encoded
T9251	5881-5890	VBN	denotes	motorized
T9252	5891-5892	NNP	denotes	Z
T9253	5892-5893	HYPH	denotes	-
T9250	5893-5898	NNP	denotes	Stage
T9254	5899-5900	-LRB-	denotes	(
T9256	5900-5905	NNP	denotes	Prior
T9255	5906-5916	NNP	denotes	Scientific
T9257	5916-5918	,	denotes	,
T9258	5918-5926	NNP	denotes	Rockland
T9259	5926-5928	,	denotes	,
T9260	5928-5936	NNP	denotes	Maryland
T9261	5936-5938	,	denotes	,
T9262	5938-5944	NNP	denotes	United
T9263	5945-5951	NNP	denotes	States
T9264	5951-5952	-RRB-	denotes	)
T9265	5952-5953	.	denotes	.
T9266	5953-6105	sentence	denotes	Images with the inverted Nikon TE2000U microscope were obtained using a CCD camera (CoolSNAP HQ; Roper Scientific, Trenton, New Jersey, United States).
T9267	5954-5960	NNS	denotes	Images
T9269	5961-5965	IN	denotes	with
T9270	5966-5969	DT	denotes	the
T9272	5970-5978	VBN	denotes	inverted
T9273	5979-5984	NNP	denotes	Nikon
T9274	5985-5992	NN	denotes	TE2000U
T9271	5993-6003	NN	denotes	microscope
T9275	6004-6008	VBD	denotes	were
T9268	6009-6017	VBN	denotes	obtained
T9276	6018-6023	VBG	denotes	using
T9277	6024-6025	DT	denotes	a
T9279	6026-6029	NN	denotes	CCD
T9278	6030-6036	NN	denotes	camera
T9280	6037-6038	-LRB-	denotes	(
T9281	6038-6046	NNP	denotes	CoolSNAP
T9282	6047-6049	NNP	denotes	HQ
T9283	6049-6050	:	denotes	;
T9285	6051-6056	NNP	denotes	Roper
T9284	6057-6067	NNP	denotes	Scientific
T9286	6067-6069	,	denotes	,
T9287	6069-6076	NNP	denotes	Trenton
T9288	6076-6078	,	denotes	,
T9289	6078-6081	NNP	denotes	New
T9290	6082-6088	NNP	denotes	Jersey
T9291	6088-6090	,	denotes	,
T9292	6090-6096	NNP	denotes	United
T9293	6097-6103	NNP	denotes	States
T9294	6103-6104	-RRB-	denotes	)
T9295	6104-6105	.	denotes	.
T9296	6105-6261	sentence	denotes	Images acquired under identical acquisition parameters were analyzed using Metamorph Software v6.2 (Universal Imaging, Glendale, Wisconsin, United States).
T9297	6106-6112	NNS	denotes	Images
T9299	6113-6121	VBN	denotes	acquired
T9300	6122-6127	IN	denotes	under
T9301	6128-6137	JJ	denotes	identical
T9303	6138-6149	NN	denotes	acquisition
T9302	6150-6160	NNS	denotes	parameters
T9304	6161-6165	VBD	denotes	were
T9298	6166-6174	VBN	denotes	analyzed
T9305	6175-6180	VBG	denotes	using
T9306	6181-6190	NNP	denotes	Metamorph
T9308	6191-6199	NNP	denotes	Software
T9307	6200-6204	NN	denotes	v6.2
T9309	6205-6206	-LRB-	denotes	(
T9311	6206-6215	NNP	denotes	Universal
T9310	6216-6223	NNP	denotes	Imaging
T9312	6223-6225	,	denotes	,
T9313	6225-6233	NNP	denotes	Glendale
T9314	6233-6235	,	denotes	,
T9315	6235-6244	NNP	denotes	Wisconsin
T9316	6244-6246	,	denotes	,
T9317	6246-6252	NNP	denotes	United
T9318	6253-6259	NNP	denotes	States
T9319	6259-6260	-RRB-	denotes	)
T9320	6260-6261	.	denotes	.
T9321	6261-6450	sentence	denotes	Whenever applicable, serial optical Z-stacks (20–30 focal planes at 100-nm intervals) were captured and computationally processed by 3-D blind deconvolution methods with the same software.
T9322	6262-6270	RB	denotes	Whenever
T9323	6271-6281	JJ	denotes	applicable
T9325	6281-6283	,	denotes	,
T9326	6283-6289	JJ	denotes	serial
T9328	6290-6297	JJ	denotes	optical
T9329	6298-6299	NN	denotes	Z
T9330	6299-6300	HYPH	denotes	-
T9327	6300-6306	NNS	denotes	stacks
T9331	6307-6308	-LRB-	denotes	(
T9332	6308-6310	CD	denotes	20
T9334	6310-6311	SYM	denotes	–
T9333	6311-6313	CD	denotes	30
T9336	6314-6319	JJ	denotes	focal
T9335	6320-6326	NNS	denotes	planes
T9337	6327-6329	IN	denotes	at
T9338	6330-6333	CD	denotes	100
T9340	6333-6334	HYPH	denotes	-
T9339	6334-6336	NN	denotes	nm
T9341	6337-6346	NNS	denotes	intervals
T9342	6346-6347	-RRB-	denotes	)
T9343	6348-6352	VBD	denotes	were
T9324	6353-6361	VBN	denotes	captured
T9344	6362-6365	CC	denotes	and
T9345	6366-6381	RB	denotes	computationally
T9346	6382-6391	VBN	denotes	processed
T9347	6392-6394	IN	denotes	by
T9348	6395-6396	CD	denotes	3
T9350	6396-6397	HYPH	denotes	-
T9349	6397-6398	NN	denotes	D
T9352	6399-6404	JJ	denotes	blind
T9353	6405-6418	NN	denotes	deconvolution
T9351	6419-6426	NNS	denotes	methods
T9354	6427-6431	IN	denotes	with
T9355	6432-6435	DT	denotes	the
T9357	6436-6440	JJ	denotes	same
T9356	6441-6449	NN	denotes	software
T9358	6449-6450	.	denotes	.
T9491	6452-6462	NN	denotes	Generation
T9492	6463-6465	IN	denotes	of
T9493	6466-6467	DT	denotes	a
T9495	6468-6473	NN	denotes	mouse
T9494	6474-6478	NN	denotes	line
T9496	6479-6483	IN	denotes	with
T9497	6484-6485	DT	denotes	a
T9499	6486-6495	VBN	denotes	disrupted
T9500	6496-6502	NN	denotes	RanBP2
T9498	6503-6508	NN	denotes	locus
T9501	6509-6512	CC	denotes	and
T9502	6513-6525	JJ	denotes	histological
T9503	6526-6534	NN	denotes	analysis
T9504	6535-6537	IN	denotes	of
T9505	6538-6545	VBN	denotes	trapped
T9507	6546-6552	NN	denotes	RanBP2
T9506	6553-6557	NNS	denotes	mice
T9508	6557-6558	.	denotes	.
T9509	6558-6897	sentence	denotes	A feeder-independent mouse ES cell line derived from the 129P2/OlaHsd strain and harboring a disrupted RanBP2 locus by insertion mutagenesis with the gene trap vector, pGT0pfs [67,68] (generated by W. Skarnes), was obtained from the Mutant Mouse Regional Resource Center (University of California Davis, Davis, California, United States).
T9510	6559-6560	DT	denotes	A
T9512	6561-6567	NN	denotes	feeder
T9514	6567-6568	HYPH	denotes	-
T9513	6568-6579	JJ	denotes	independent
T9515	6580-6585	NN	denotes	mouse
T9516	6586-6588	NN	denotes	ES
T9517	6589-6593	NN	denotes	cell
T9511	6594-6598	NN	denotes	line
T9519	6599-6606	VBN	denotes	derived
T9520	6607-6611	IN	denotes	from
T9521	6612-6615	DT	denotes	the
T9523	6616-6621	NN	denotes	129P2
T9525	6621-6622	HYPH	denotes	/
T9524	6622-6628	NN	denotes	OlaHsd
T9522	6629-6635	NN	denotes	strain
T9526	6636-6639	CC	denotes	and
T9527	6640-6649	VBG	denotes	harboring
T9528	6650-6651	DT	denotes	a
T9530	6652-6661	VBN	denotes	disrupted
T9531	6662-6668	NN	denotes	RanBP2
T9529	6669-6674	NN	denotes	locus
T9532	6675-6677	IN	denotes	by
T9533	6678-6687	NN	denotes	insertion
T9534	6688-6699	NN	denotes	mutagenesis
T9535	6700-6704	IN	denotes	with
T9536	6705-6708	DT	denotes	the
T9538	6709-6713	NN	denotes	gene
T9539	6714-6718	NN	denotes	trap
T9537	6719-6725	NN	denotes	vector
T9540	6725-6727	,	denotes	,
T9541	6727-6734	NN	denotes	pGT0pfs
T9542	6735-6736	-LRB-	denotes	[
T9544	6736-6738	CD	denotes	67
T9545	6738-6739	,	denotes	,
T9543	6739-6741	CD	denotes	68
T9546	6741-6742	-RRB-	denotes	]
T9547	6743-6744	-LRB-	denotes	(
T9548	6744-6753	VBN	denotes	generated
T9549	6754-6756	IN	denotes	by
T9550	6757-6759	NNP	denotes	W.
T9551	6760-6767	NNP	denotes	Skarnes
T9552	6767-6768	-RRB-	denotes	)
T9553	6768-6770	,	denotes	,
T9554	6770-6773	VBD	denotes	was
T9518	6774-6782	VBN	denotes	obtained
T9555	6783-6787	IN	denotes	from
T9556	6788-6791	DT	denotes	the
T9558	6792-6798	NNP	denotes	Mutant
T9559	6799-6804	NNP	denotes	Mouse
T9560	6805-6813	NNP	denotes	Regional
T9561	6814-6822	NNP	denotes	Resource
T9557	6823-6829	NNP	denotes	Center
T9562	6830-6831	-LRB-	denotes	(
T9563	6831-6841	NNP	denotes	University
T9564	6842-6844	IN	denotes	of
T9565	6845-6855	NNP	denotes	California
T9566	6856-6861	NNP	denotes	Davis
T9567	6861-6863	,	denotes	,
T9568	6863-6868	NNP	denotes	Davis
T9569	6868-6870	,	denotes	,
T9570	6870-6880	NNP	denotes	California
T9571	6880-6882	,	denotes	,
T9572	6882-6888	NNP	denotes	United
T9573	6889-6895	NNP	denotes	States
T9574	6895-6896	-RRB-	denotes	)
T9575	6896-6897	.	denotes	.
T9576	6897-7127	sentence	denotes	The gene trap vector contains a promoterless neo-lacZ fusion gene with a splice acceptor site at the 5′ end followed by an internal ribosome entry site (IRES) and the human placental alkaline phosphatase (PLAP) reporter cassette.
T9577	6898-6901	DT	denotes	The
T9579	6902-6906	NN	denotes	gene
T9580	6907-6911	NN	denotes	trap
T9578	6912-6918	NN	denotes	vector
T9581	6919-6927	VBZ	denotes	contains
T9582	6928-6929	DT	denotes	a
T9584	6930-6942	JJ	denotes	promoterless
T9585	6943-6951	NN	denotes	neo-lacZ
T9586	6952-6958	NN	denotes	fusion
T9583	6959-6963	NN	denotes	gene
T9587	6964-6968	IN	denotes	with
T9588	6969-6970	DT	denotes	a
T9590	6971-6977	NN	denotes	splice
T9591	6978-6986	NN	denotes	acceptor
T9589	6987-6991	NN	denotes	site
T9592	6992-6994	IN	denotes	at
T9593	6995-6998	DT	denotes	the
T9595	6999-7000	CD	denotes	5
T9596	7000-7001	SYM	denotes	′
T9594	7002-7005	NN	denotes	end
T9597	7006-7014	VBN	denotes	followed
T9598	7015-7017	IN	denotes	by
T9599	7018-7020	DT	denotes	an
T9601	7021-7029	JJ	denotes	internal
T9602	7030-7038	NN	denotes	ribosome
T9603	7039-7044	NN	denotes	entry
T9600	7045-7049	NN	denotes	site
T9604	7050-7051	-LRB-	denotes	(
T9605	7051-7055	NN	denotes	IRES
T9606	7055-7056	-RRB-	denotes	)
T9607	7057-7060	CC	denotes	and
T9608	7061-7064	DT	denotes	the
T9610	7065-7070	JJ	denotes	human
T9611	7071-7080	JJ	denotes	placental
T9613	7081-7089	NN	denotes	alkaline
T9612	7090-7101	NN	denotes	phosphatase
T9614	7102-7103	-LRB-	denotes	(
T9615	7103-7107	NN	denotes	PLAP
T9616	7107-7108	-RRB-	denotes	)
T9617	7109-7117	NN	denotes	reporter
T9609	7118-7126	NN	denotes	cassette
T9618	7126-7127	.	denotes	.
T9619	7127-7231	sentence	denotes	The RanBP2 129Ola ES line was injected into C57BL/6J blastocysts and four male chimeras were generated.
T9620	7128-7131	DT	denotes	The
T9622	7132-7138	NN	denotes	RanBP2
T9623	7139-7145	NN	denotes	129Ola
T9624	7146-7148	NN	denotes	ES
T9621	7149-7153	NN	denotes	line
T9626	7154-7157	VBD	denotes	was
T9625	7158-7166	VBN	denotes	injected
T9627	7167-7171	IN	denotes	into
T9628	7172-7177	NN	denotes	C57BL
T9630	7177-7178	HYPH	denotes	/
T9629	7178-7180	NN	denotes	6J
T9631	7181-7192	NNS	denotes	blastocysts
T9632	7193-7196	CC	denotes	and
T9633	7197-7201	CD	denotes	four
T9635	7202-7206	JJ	denotes	male
T9634	7207-7215	NNS	denotes	chimeras
T9637	7216-7220	VBD	denotes	were
T9636	7221-7230	VBN	denotes	generated
T9638	7230-7231	.	denotes	.
T9639	7231-7389	sentence	denotes	These were bred into 129P2/OlaHsd (Harlan, Indianapolis, Indiana, United States) and C57BL/6J (Jackson Laboratory, Bar Harbor, Maine, United States) females.
T9640	7232-7237	DT	denotes	These
T9642	7238-7242	VBD	denotes	were
T9641	7243-7247	VBN	denotes	bred
T9643	7248-7252	IN	denotes	into
T9644	7253-7258	NN	denotes	129P2
T9646	7258-7259	HYPH	denotes	/
T9645	7259-7265	NN	denotes	OlaHsd
T9648	7266-7267	-LRB-	denotes	(
T9649	7267-7273	NNP	denotes	Harlan
T9650	7273-7275	,	denotes	,
T9651	7275-7287	NNP	denotes	Indianapolis
T9652	7287-7289	,	denotes	,
T9653	7289-7296	NNP	denotes	Indiana
T9654	7296-7298	,	denotes	,
T9655	7298-7304	NNP	denotes	United
T9656	7305-7311	NNP	denotes	States
T9657	7311-7312	-RRB-	denotes	)
T9658	7313-7316	CC	denotes	and
T9659	7317-7322	NN	denotes	C57BL
T9661	7322-7323	HYPH	denotes	/
T9660	7323-7325	NN	denotes	6J
T9662	7326-7327	-LRB-	denotes	(
T9664	7327-7334	NNP	denotes	Jackson
T9663	7335-7345	NNP	denotes	Laboratory
T9665	7345-7347	,	denotes	,
T9666	7347-7350	NNP	denotes	Bar
T9667	7351-7357	NNP	denotes	Harbor
T9668	7357-7359	,	denotes	,
T9669	7359-7364	NNP	denotes	Maine
T9670	7364-7366	,	denotes	,
T9671	7366-7372	NNP	denotes	United
T9672	7373-7379	NNP	denotes	States
T9673	7379-7380	-RRB-	denotes	)
T9647	7381-7388	NNS	denotes	females
T9674	7388-7389	.	denotes	.
T9675	7389-7605	sentence	denotes	The two resulting and independent F1s lines, with 129P2/OlaHsd inbred and 129P2/OlaHsd/C57BL/6J mixed genetic backgrounds, were tested for germline transmission by PCR and Southern blot analysis of tail genomic DNA.
T9676	7390-7393	DT	denotes	The
T9678	7394-7397	CD	denotes	two
T9679	7398-7407	VBG	denotes	resulting
T9680	7408-7411	CC	denotes	and
T9681	7412-7423	JJ	denotes	independent
T9682	7424-7427	NN	denotes	F1s
T9677	7428-7433	NNS	denotes	lines
T9684	7433-7435	,	denotes	,
T9685	7435-7439	IN	denotes	with
T9686	7440-7445	NN	denotes	129P2
T9688	7445-7446	HYPH	denotes	/
T9687	7446-7452	NN	denotes	OlaHsd
T9689	7453-7459	JJ	denotes	inbred
T9690	7460-7463	CC	denotes	and
T9691	7464-7469	NN	denotes	129P2
T9693	7469-7470	HYPH	denotes	/
T9694	7470-7476	NN	denotes	OlaHsd
T9695	7476-7477	HYPH	denotes	/
T9696	7477-7482	NN	denotes	C57BL
T9697	7482-7483	HYPH	denotes	/
T9692	7483-7485	NN	denotes	6J
T9699	7486-7491	JJ	denotes	mixed
T9700	7492-7499	JJ	denotes	genetic
T9698	7500-7511	NNS	denotes	backgrounds
T9701	7511-7513	,	denotes	,
T9702	7513-7517	VBD	denotes	were
T9683	7518-7524	VBN	denotes	tested
T9703	7525-7528	IN	denotes	for
T9704	7529-7537	NN	denotes	germline
T9705	7538-7550	NN	denotes	transmission
T9706	7551-7553	IN	denotes	by
T9707	7554-7557	NN	denotes	PCR
T9709	7558-7561	CC	denotes	and
T9710	7562-7570	NNP	denotes	Southern
T9711	7571-7575	NN	denotes	blot
T9708	7576-7584	NN	denotes	analysis
T9712	7585-7587	IN	denotes	of
T9713	7588-7592	NN	denotes	tail
T9715	7593-7600	JJ	denotes	genomic
T9714	7601-7604	NN	denotes	DNA
T9716	7604-7605	.	denotes	.
T9717	7605-7960	sentence	denotes	β-gal and PLAP activities in whole mount embryos and retinal sections were detected, respectively, by incubation with 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside and AP staining buffer (0.1 mg/ml 5-bromo-4-chloro-3-indolyl phosphate, 1 mg/ml nitroblue tetrazolium in 100 mM Tris-HCl (pH 9.5), 100 mM NaCl, 5 mM MgCl2) as described elsewhere [67,68].
T9718	7606-7607	NN	denotes	β
T9720	7607-7608	HYPH	denotes	-
T9719	7608-7611	NN	denotes	gal
T9722	7612-7615	CC	denotes	and
T9723	7616-7620	NN	denotes	PLAP
T9721	7621-7631	NNS	denotes	activities
T9725	7632-7634	IN	denotes	in
T9726	7635-7640	JJ	denotes	whole
T9727	7641-7646	NN	denotes	mount
T9728	7647-7654	NNS	denotes	embryos
T9729	7655-7658	CC	denotes	and
T9730	7659-7666	JJ	denotes	retinal
T9731	7667-7675	NNS	denotes	sections
T9732	7676-7680	VBD	denotes	were
T9724	7681-7689	VBN	denotes	detected
T9733	7689-7691	,	denotes	,
T9734	7691-7703	RB	denotes	respectively
T9735	7703-7705	,	denotes	,
T9736	7705-7707	IN	denotes	by
T9737	7708-7718	NN	denotes	incubation
T9738	7719-7723	IN	denotes	with
T9739	7724-7725	CD	denotes	5
T9741	7725-7726	HYPH	denotes	-
T9740	7726-7731	NN	denotes	bromo
T9743	7731-7732	HYPH	denotes	-
T9744	7732-7733	CD	denotes	4
T9745	7733-7734	HYPH	denotes	-
T9746	7734-7740	NN	denotes	chloro
T9747	7740-7741	HYPH	denotes	-
T9748	7741-7742	CD	denotes	3
T9750	7742-7743	HYPH	denotes	-
T9751	7743-7750	NN	denotes	indolyl
T9749	7751-7752	NN	denotes	β
T9752	7752-7753	HYPH	denotes	-
T9753	7753-7754	NN	denotes	D
T9754	7754-7755	HYPH	denotes	-
T9742	7755-7772	NN	denotes	galactopyranoside
T9756	7773-7776	CC	denotes	and
T9757	7777-7779	NN	denotes	AP
T9758	7780-7788	NN	denotes	staining
T9755	7789-7795	NN	denotes	buffer
T9759	7796-7797	-LRB-	denotes	(
T9761	7797-7800	CD	denotes	0.1
T9762	7801-7803	NNS	denotes	mg
T9764	7803-7804	SYM	denotes	/
T9765	7804-7806	NN	denotes	ml
T9766	7807-7808	CD	denotes	5
T9768	7808-7809	HYPH	denotes	-
T9767	7809-7814	NN	denotes	bromo
T9769	7814-7815	HYPH	denotes	-
T9770	7815-7816	CD	denotes	4
T9772	7816-7817	HYPH	denotes	-
T9771	7817-7823	NN	denotes	chloro
T9773	7823-7824	HYPH	denotes	-
T9774	7824-7825	CD	denotes	3
T9775	7825-7826	HYPH	denotes	-
T9776	7826-7833	NN	denotes	indolyl
T9763	7834-7843	NN	denotes	phosphate
T9777	7843-7845	,	denotes	,
T9778	7845-7846	CD	denotes	1
T9779	7847-7849	NNS	denotes	mg
T9780	7849-7850	SYM	denotes	/
T9781	7850-7852	NN	denotes	ml
T9782	7853-7862	JJ	denotes	nitroblue
T9760	7863-7874	NN	denotes	tetrazolium
T9783	7875-7877	IN	denotes	in
T9784	7878-7881	CD	denotes	100
T9785	7882-7884	NNS	denotes	mM
T9787	7885-7889	NN	denotes	Tris
T9788	7889-7890	HYPH	denotes	-
T9786	7890-7893	NN	denotes	HCl
T9789	7894-7895	-LRB-	denotes	(
T9790	7895-7897	NN	denotes	pH
T9791	7898-7901	CD	denotes	9.5
T9792	7901-7902	-RRB-	denotes	)
T9793	7902-7904	,	denotes	,
T9794	7904-7907	CD	denotes	100
T9795	7908-7910	NNS	denotes	mM
T9796	7911-7915	NN	denotes	NaCl
T9797	7915-7917	,	denotes	,
T9798	7917-7918	CD	denotes	5
T9799	7919-7921	NNS	denotes	mM
T9800	7922-7927	NN	denotes	MgCl2
T9801	7927-7928	-RRB-	denotes	)
T9802	7929-7931	IN	denotes	as
T9803	7932-7941	VBN	denotes	described
T9804	7942-7951	RB	denotes	elsewhere
T9805	7952-7953	-LRB-	denotes	[
T9807	7953-7955	CD	denotes	67
T9808	7955-7956	,	denotes	,
T9806	7956-7958	CD	denotes	68
T9809	7958-7959	-RRB-	denotes	]
T9810	7959-7960	.	denotes	.
T9862	7962-7969	NNP	denotes	Western
T9863	7970-7974	NN	denotes	blot
T9864	7975-7978	CC	denotes	and
T9865	7979-7991	NN	denotes	quantitation
T9866	7992-8000	NN	denotes	analysis
T9867	8001-8003	IN	denotes	of
T9868	8004-8011	NN	denotes	protein
T9869	8012-8022	NN	denotes	expression
T9870	8023-8026	CC	denotes	and
T9871	8027-8030	NN	denotes	ATP
T9872	8031-8037	NNS	denotes	levels
T9873	8037-8038	.	denotes	.
T9874	8038-8155	sentence	denotes	Homogenates and detergent solubilized extracts of tissues and immunoblots were prepared as described elsewhere [69].
T9875	8039-8050	NNS	denotes	Homogenates
T9877	8051-8054	CC	denotes	and
T9878	8055-8064	NN	denotes	detergent
T9879	8065-8076	VBN	denotes	solubilized
T9880	8077-8085	NNS	denotes	extracts
T9881	8086-8088	IN	denotes	of
T9882	8089-8096	NNS	denotes	tissues
T9883	8097-8100	CC	denotes	and
T9884	8101-8112	NNS	denotes	immunoblots
T9885	8113-8117	VBD	denotes	were
T9876	8118-8126	VBN	denotes	prepared
T9886	8127-8129	IN	denotes	as
T9887	8130-8139	VBN	denotes	described
T9888	8140-8149	RB	denotes	elsewhere
T9889	8150-8151	-LRB-	denotes	[
T9890	8151-8153	CD	denotes	69
T9891	8153-8154	-RRB-	denotes	]
T9892	8154-8155	.	denotes	.
T9893	8155-8353	sentence	denotes	Quantitation of immunoblot bands calibrated against internal marker bands, such as mHsp70 and Nup153, was carried out with Gel Pro Analyzer (MediaCybernetics, San Diego, California, United States).
T9894	8156-8168	NN	denotes	Quantitation
T9896	8169-8171	IN	denotes	of
T9897	8172-8182	NN	denotes	immunoblot
T9898	8183-8188	NNS	denotes	bands
T9899	8189-8199	VBN	denotes	calibrated
T9900	8200-8207	IN	denotes	against
T9901	8208-8216	JJ	denotes	internal
T9903	8217-8223	NN	denotes	marker
T9902	8224-8229	NNS	denotes	bands
T9904	8229-8231	,	denotes	,
T9905	8231-8235	JJ	denotes	such
T9906	8236-8238	IN	denotes	as
T9907	8239-8245	NN	denotes	mHsp70
T9908	8246-8249	CC	denotes	and
T9909	8250-8256	NN	denotes	Nup153
T9910	8256-8258	,	denotes	,
T9911	8258-8261	VBD	denotes	was
T9895	8262-8269	VBN	denotes	carried
T9912	8270-8273	RP	denotes	out
T9913	8274-8278	IN	denotes	with
T9914	8279-8282	NNP	denotes	Gel
T9916	8283-8286	NNP	denotes	Pro
T9915	8287-8295	NNP	denotes	Analyzer
T9917	8296-8297	-LRB-	denotes	(
T9918	8297-8313	NNPS	denotes	MediaCybernetics
T9919	8313-8315	,	denotes	,
T9920	8315-8318	NNP	denotes	San
T9921	8319-8324	NNP	denotes	Diego
T9922	8324-8326	,	denotes	,
T9923	8326-8336	NNP	denotes	California
T9924	8336-8338	,	denotes	,
T9925	8338-8344	NNP	denotes	United
T9926	8345-8351	NNP	denotes	States
T9927	8351-8352	-RRB-	denotes	)
T9928	8352-8353	.	denotes	.
T9929	8353-8569	sentence	denotes	For determination of ATP levels, freshly dissected and flash-frozen tissues were homogenized in 2.5% trichloroacetic acid (TCA), neutralized, and diluted to a final concentration of 0.1% with Tris-Acetate (pH 7.75).
T9930	8354-8357	IN	denotes	For
T9932	8358-8371	NN	denotes	determination
T9933	8372-8374	IN	denotes	of
T9934	8375-8378	NN	denotes	ATP
T9935	8379-8385	NNS	denotes	levels
T9936	8385-8387	,	denotes	,
T9937	8387-8394	RB	denotes	freshly
T9938	8395-8404	VBN	denotes	dissected
T9940	8405-8408	CC	denotes	and
T9941	8409-8414	NN	denotes	flash
T9943	8414-8415	HYPH	denotes	-
T9942	8415-8421	VBN	denotes	frozen
T9939	8422-8429	NNS	denotes	tissues
T9944	8430-8434	VBD	denotes	were
T9931	8435-8446	VBN	denotes	homogenized
T9945	8447-8449	IN	denotes	in
T9946	8450-8453	CD	denotes	2.5
T9947	8453-8454	NN	denotes	%
T9948	8455-8470	JJ	denotes	trichloroacetic
T9949	8471-8475	NN	denotes	acid
T9950	8476-8477	-LRB-	denotes	(
T9951	8477-8480	NN	denotes	TCA
T9952	8480-8481	-RRB-	denotes	)
T9953	8481-8483	,	denotes	,
T9954	8483-8494	VBN	denotes	neutralized
T9955	8494-8496	,	denotes	,
T9956	8496-8499	CC	denotes	and
T9957	8500-8507	VBD	denotes	diluted
T9958	8508-8510	IN	denotes	to
T9959	8511-8512	DT	denotes	a
T9961	8513-8518	JJ	denotes	final
T9960	8519-8532	NN	denotes	concentration
T9962	8533-8535	IN	denotes	of
T9963	8536-8539	CD	denotes	0.1
T9964	8539-8540	NN	denotes	%
T9965	8541-8545	IN	denotes	with
T9966	8546-8550	NN	denotes	Tris
T9968	8550-8551	HYPH	denotes	-
T9967	8551-8558	NN	denotes	Acetate
T9969	8559-8560	-LRB-	denotes	(
T9970	8560-8562	NN	denotes	pH
T9971	8563-8567	CD	denotes	7.75
T9972	8567-8568	-RRB-	denotes	)
T9973	8568-8569	.	denotes	.
T9974	8569-8834	sentence	denotes	ATP measurements were carried out with the ENLITEN ATP Assay System Bioluminescence Detection Kit for ATP (Promega, Madison, Wisconsin, United States) as per the manufacturer's instruction in SpectraMax-M5 (Molecular Devices, Sunnyvale, California, United States).
T9975	8570-8573	NN	denotes	ATP
T9976	8574-8586	NNS	denotes	measurements
T9978	8587-8591	VBD	denotes	were
T9977	8592-8599	VBN	denotes	carried
T9979	8600-8603	RP	denotes	out
T9980	8604-8608	IN	denotes	with
T9981	8609-8612	DT	denotes	the
T9983	8613-8620	NNP	denotes	ENLITEN
T9984	8621-8624	NNP	denotes	ATP
T9986	8625-8630	NNP	denotes	Assay
T9985	8631-8637	NNP	denotes	System
T9987	8638-8653	NNP	denotes	Bioluminescence
T9988	8654-8663	NNP	denotes	Detection
T9982	8664-8667	NNP	denotes	Kit
T9989	8668-8671	IN	denotes	for
T9990	8672-8675	NN	denotes	ATP
T9991	8676-8677	-LRB-	denotes	(
T9992	8677-8684	NNP	denotes	Promega
T9993	8684-8686	,	denotes	,
T9994	8686-8693	NNP	denotes	Madison
T9995	8693-8695	,	denotes	,
T9996	8695-8704	NNP	denotes	Wisconsin
T9997	8704-8706	,	denotes	,
T9998	8706-8712	NNP	denotes	United
T9999	8713-8719	NNP	denotes	States
T10000	8719-8720	-RRB-	denotes	)
T10001	8721-8723	IN	denotes	as
T10002	8724-8727	IN	denotes	per
T10003	8728-8731	DT	denotes	the
T10004	8732-8744	NN	denotes	manufacturer
T10006	8744-8746	POS	denotes	's
T10005	8747-8758	NN	denotes	instruction
T10007	8759-8761	IN	denotes	in
T10008	8762-8772	NNP	denotes	SpectraMax
T10010	8772-8773	HYPH	denotes	-
T10009	8773-8775	NNP	denotes	M5
T10011	8776-8777	-LRB-	denotes	(
T10013	8777-8786	NNP	denotes	Molecular
T10012	8787-8794	NNPS	denotes	Devices
T10014	8794-8796	,	denotes	,
T10015	8796-8805	NNP	denotes	Sunnyvale
T10016	8805-8807	,	denotes	,
T10017	8807-8817	NNP	denotes	California
T10018	8817-8819	,	denotes	,
T10019	8819-8825	NNP	denotes	United
T10020	8826-8832	NNP	denotes	States
T10021	8832-8833	-RRB-	denotes	)
T10022	8833-8834	.	denotes	.
T10023	8834-8958	sentence	denotes	Protein concentration was measured by the BCA method (BioRad, Hercules, California, United States) using BSA as a standard.
T10024	8835-8842	NN	denotes	Protein
T10025	8843-8856	NN	denotes	concentration
T10027	8857-8860	VBD	denotes	was
T10026	8861-8869	VBN	denotes	measured
T10028	8870-8872	IN	denotes	by
T10029	8873-8876	DT	denotes	the
T10031	8877-8880	NN	denotes	BCA
T10030	8881-8887	NN	denotes	method
T10032	8888-8889	-LRB-	denotes	(
T10033	8889-8895	NNP	denotes	BioRad
T10034	8895-8897	,	denotes	,
T10035	8897-8905	NNP	denotes	Hercules
T10036	8905-8907	,	denotes	,
T10037	8907-8917	NNP	denotes	California
T10038	8917-8919	,	denotes	,
T10039	8919-8925	NNP	denotes	United
T10040	8926-8932	NNP	denotes	States
T10041	8932-8933	-RRB-	denotes	)
T10042	8934-8939	VBG	denotes	using
T10043	8940-8943	NN	denotes	BSA
T10044	8944-8946	IN	denotes	as
T10045	8947-8948	DT	denotes	a
T10046	8949-8957	NN	denotes	standard
T10047	8957-8958	.	denotes	.
T10131	8960-8969	JJ	denotes	Metabolic
T10132	8970-8976	NNS	denotes	assays
T10133	8976-8977	.	denotes	.
T10134	8977-9057	sentence	denotes	Two groups of male mice were kept under separate diet since gestation or birth.
T10135	8978-8981	CD	denotes	Two
T10136	8982-8988	NNS	denotes	groups
T10138	8989-8991	IN	denotes	of
T10139	8992-8996	JJ	denotes	male
T10140	8997-9001	NNS	denotes	mice
T10141	9002-9006	VBD	denotes	were
T10137	9007-9011	VBN	denotes	kept
T10142	9012-9017	IN	denotes	under
T10143	9018-9026	JJ	denotes	separate
T10144	9027-9031	NN	denotes	diet
T10145	9032-9037	IN	denotes	since
T10146	9038-9047	NN	denotes	gestation
T10147	9048-9050	CC	denotes	or
T10148	9051-9056	NN	denotes	birth
T10149	9056-9057	.	denotes	.
T10150	9057-9273	sentence	denotes	One group was fed with a normal chow diet (PicoLab Rodent Diet 20 −5053; LabDiet, Richmond, Indiana, United States), while the other was placed on a higher energy and fat diet (PicoLab Mouse Diet 20 −5058; LabDiet).
T10151	9058-9061	CD	denotes	One
T10152	9062-9067	NN	denotes	group
T10154	9068-9071	VBD	denotes	was
T10153	9072-9075	VBN	denotes	fed
T10155	9076-9080	IN	denotes	with
T10156	9081-9082	DT	denotes	a
T10158	9083-9089	JJ	denotes	normal
T10159	9090-9094	NN	denotes	chow
T10157	9095-9099	NN	denotes	diet
T10160	9100-9101	-LRB-	denotes	(
T10161	9101-9108	NNP	denotes	PicoLab
T10163	9109-9115	NNP	denotes	Rodent
T10162	9116-9120	NNP	denotes	Diet
T10164	9121-9123	CD	denotes	20
T10165	9124-9125	HYPH	denotes	−
T10166	9125-9129	CD	denotes	5053
T10167	9129-9130	:	denotes	;
T10168	9131-9138	NNP	denotes	LabDiet
T10169	9138-9140	,	denotes	,
T10170	9140-9148	NNP	denotes	Richmond
T10171	9148-9150	,	denotes	,
T10172	9150-9157	NNP	denotes	Indiana
T10173	9157-9159	,	denotes	,
T10174	9159-9165	NNP	denotes	United
T10175	9166-9172	NNP	denotes	States
T10176	9172-9173	-RRB-	denotes	)
T10177	9173-9175	,	denotes	,
T10178	9175-9180	IN	denotes	while
T10180	9181-9184	DT	denotes	the
T10181	9185-9190	NN	denotes	other
T10182	9191-9194	VBD	denotes	was
T10179	9195-9201	VBN	denotes	placed
T10183	9202-9204	IN	denotes	on
T10184	9205-9206	DT	denotes	a
T10186	9207-9213	JJR	denotes	higher
T10187	9214-9220	NN	denotes	energy
T10188	9221-9224	CC	denotes	and
T10189	9225-9228	NN	denotes	fat
T10185	9229-9233	NN	denotes	diet
T10190	9234-9235	-LRB-	denotes	(
T10191	9235-9242	NNP	denotes	PicoLab
T10193	9243-9248	NNP	denotes	Mouse
T10192	9249-9253	NNP	denotes	Diet
T10194	9254-9256	CD	denotes	20
T10195	9257-9258	HYPH	denotes	−
T10196	9258-9262	CD	denotes	5058
T10197	9262-9263	:	denotes	;
T10198	9264-9271	NNP	denotes	LabDiet
T10199	9271-9272	-RRB-	denotes	)
T10200	9272-9273	.	denotes	.
T10201	9273-9405	sentence	denotes	Glucose tolerance test was performed on overnight-fasted mice injected intraperitoneally with 2 g of glucose per kg of body weight.
T10202	9274-9281	NN	denotes	Glucose
T10203	9282-9291	NN	denotes	tolerance
T10204	9292-9296	NN	denotes	test
T10206	9297-9300	VBD	denotes	was
T10205	9301-9310	VBN	denotes	performed
T10207	9311-9313	IN	denotes	on
T10208	9314-9323	JJ	denotes	overnight
T10210	9323-9324	HYPH	denotes	-
T10209	9324-9330	VBN	denotes	fasted
T10211	9331-9335	NNS	denotes	mice
T10212	9336-9344	VBN	denotes	injected
T10213	9345-9362	RB	denotes	intraperitoneally
T10214	9363-9367	IN	denotes	with
T10215	9368-9369	CD	denotes	2
T10216	9370-9371	NNS	denotes	g
T10217	9372-9374	IN	denotes	of
T10218	9375-9382	NN	denotes	glucose
T10219	9383-9386	IN	denotes	per
T10220	9387-9389	NN	denotes	kg
T10221	9390-9392	IN	denotes	of
T10222	9393-9397	NN	denotes	body
T10223	9398-9404	NN	denotes	weight
T10224	9404-9405	.	denotes	.
T10225	9405-9602	sentence	denotes	Venous blood glucose values were determined at immediately before (0 min), and 15, 30, 60, and 120 min after the injection with an automatic glucose monitor instrument (One Touch Ultra, Lifescan).
T10226	9406-9412	JJ	denotes	Venous
T10228	9413-9418	NN	denotes	blood
T10229	9419-9426	NN	denotes	glucose
T10227	9427-9433	NNS	denotes	values
T10231	9434-9438	VBD	denotes	were
T10230	9439-9449	VBN	denotes	determined
T10232	9450-9452	IN	denotes	at
T10233	9453-9464	RB	denotes	immediately
T10234	9465-9471	IN	denotes	before
T10235	9472-9473	-LRB-	denotes	(
T10237	9473-9474	CD	denotes	0
T10236	9475-9478	NNS	denotes	min
T10238	9478-9479	-RRB-	denotes	)
T10239	9479-9481	,	denotes	,
T10240	9481-9484	CC	denotes	and
T10241	9485-9487	CD	denotes	15
T10243	9487-9489	,	denotes	,
T10244	9489-9491	CD	denotes	30
T10245	9491-9493	,	denotes	,
T10246	9493-9495	CD	denotes	60
T10247	9495-9497	,	denotes	,
T10248	9497-9500	CC	denotes	and
T10249	9501-9504	CD	denotes	120
T10242	9505-9508	NN	denotes	min
T10251	9509-9514	IN	denotes	after
T10252	9515-9518	DT	denotes	the
T10250	9519-9528	NN	denotes	injection
T10253	9529-9533	IN	denotes	with
T10254	9534-9536	DT	denotes	an
T10256	9537-9546	JJ	denotes	automatic
T10257	9547-9554	NN	denotes	glucose
T10258	9555-9562	NN	denotes	monitor
T10255	9563-9573	NN	denotes	instrument
T10259	9574-9575	-LRB-	denotes	(
T10260	9575-9578	CD	denotes	One
T10262	9579-9584	NNP	denotes	Touch
T10261	9585-9590	NNP	denotes	Ultra
T10263	9590-9592	,	denotes	,
T10264	9592-9600	NNP	denotes	Lifescan
T10265	9600-9601	-RRB-	denotes	)
T10266	9601-9602	.	denotes	.
T10267	9602-9815	sentence	denotes	Insulin tolerance test was performed the same way as that described for the glucose tolerance test with the exception that human insulin (0.75 U/kg of body mass) was injected intraperitoneally in non-fasted mice.
T10268	9603-9610	NN	denotes	Insulin
T10269	9611-9620	NN	denotes	tolerance
T10270	9621-9625	NN	denotes	test
T10272	9626-9629	VBD	denotes	was
T10271	9630-9639	VBN	denotes	performed
T10273	9640-9643	DT	denotes	the
T10275	9644-9648	JJ	denotes	same
T10274	9649-9652	NN	denotes	way
T10276	9653-9655	IN	denotes	as
T10277	9656-9660	DT	denotes	that
T10278	9661-9670	VBN	denotes	described
T10279	9671-9674	IN	denotes	for
T10280	9675-9678	DT	denotes	the
T10282	9679-9686	NN	denotes	glucose
T10283	9687-9696	NN	denotes	tolerance
T10281	9697-9701	NN	denotes	test
T10284	9702-9706	IN	denotes	with
T10285	9707-9710	DT	denotes	the
T10286	9711-9720	NN	denotes	exception
T10287	9721-9725	IN	denotes	that
T10289	9726-9731	JJ	denotes	human
T10290	9732-9739	NN	denotes	insulin
T10291	9740-9741	-LRB-	denotes	(
T10293	9741-9745	CD	denotes	0.75
T10292	9746-9747	NNS	denotes	U
T10294	9747-9748	SYM	denotes	/
T10295	9748-9750	NN	denotes	kg
T10296	9751-9753	IN	denotes	of
T10297	9754-9758	NN	denotes	body
T10298	9759-9763	NN	denotes	mass
T10299	9763-9764	-RRB-	denotes	)
T10300	9765-9768	VBD	denotes	was
T10288	9769-9777	VBN	denotes	injected
T10301	9778-9795	RB	denotes	intraperitoneally
T10302	9796-9798	IN	denotes	in
T10303	9799-9809	JJ	denotes	non-fasted
T10304	9810-9814	NNS	denotes	mice
T10305	9814-9815	.	denotes	.
T10306	9815-10047	sentence	denotes	Pyruvate challenge test was performed the same way as that described for the glucose tolerance test with the exception that pyruvate dissolved in saline (2 g/kg of body mass) was injected intraperitoneally in overnight-fasted mice.
T10307	9816-9824	NN	denotes	Pyruvate
T10308	9825-9834	NN	denotes	challenge
T10309	9835-9839	NN	denotes	test
T10311	9840-9843	VBD	denotes	was
T10310	9844-9853	VBN	denotes	performed
T10312	9854-9857	DT	denotes	the
T10314	9858-9862	JJ	denotes	same
T10313	9863-9866	NN	denotes	way
T10315	9867-9869	IN	denotes	as
T10316	9870-9874	DT	denotes	that
T10317	9875-9884	VBN	denotes	described
T10318	9885-9888	IN	denotes	for
T10319	9889-9892	DT	denotes	the
T10321	9893-9900	NN	denotes	glucose
T10322	9901-9910	NN	denotes	tolerance
T10320	9911-9915	NN	denotes	test
T10323	9916-9920	IN	denotes	with
T10324	9921-9924	DT	denotes	the
T10325	9925-9934	NN	denotes	exception
T10326	9935-9939	IN	denotes	that
T10328	9940-9948	NN	denotes	pyruvate
T10329	9949-9958	VBN	denotes	dissolved
T10330	9959-9961	IN	denotes	in
T10331	9962-9968	NN	denotes	saline
T10332	9969-9970	-LRB-	denotes	(
T10334	9970-9971	CD	denotes	2
T10333	9972-9973	NNS	denotes	g
T10335	9973-9974	SYM	denotes	/
T10336	9974-9976	NN	denotes	kg
T10337	9977-9979	IN	denotes	of
T10338	9980-9984	NN	denotes	body
T10339	9985-9989	NN	denotes	mass
T10340	9989-9990	-RRB-	denotes	)
T10341	9991-9994	VBD	denotes	was
T10327	9995-10003	VBN	denotes	injected
T10342	10004-10021	RB	denotes	intraperitoneally
T10343	10022-10024	IN	denotes	in
T10344	10025-10034	JJ	denotes	overnight
T10346	10034-10035	HYPH	denotes	-
T10345	10035-10041	VBN	denotes	fasted
T10347	10042-10046	NNS	denotes	mice
T10348	10046-10047	.	denotes	.
T10407	10049-10068	NN	denotes	Electroretinography
T10408	10068-10069	.	denotes	.
T10409	10069-10240	sentence	denotes	Five ~6-mo-old male RanBP2+/+ and RanBP2+/− mice in 129ola background were kept in dim cyclic light (10 lux) for 2 wk before electroretinographic responses were recorded.
T10410	10070-10074	CD	denotes	Five
T10412	10075-10076	SYM	denotes	~
T10413	10076-10077	CD	denotes	6
T10415	10077-10078	HYPH	denotes	-
T10414	10078-10080	NN	denotes	mo
T10417	10080-10081	HYPH	denotes	-
T10416	10081-10084	JJ	denotes	old
T10418	10085-10089	JJ	denotes	male
T10419	10090-10096	NN	denotes	RanBP2
T10420	10096-10097	SYM	denotes	+
T10421	10097-10098	HYPH	denotes	/
T10422	10098-10099	SYM	denotes	+
T10423	10100-10103	CC	denotes	and
T10424	10104-10110	NN	denotes	RanBP2
T10425	10110-10111	SYM	denotes	+
T10426	10111-10112	HYPH	denotes	/
T10427	10112-10113	SYM	denotes	−
T10411	10114-10118	NNS	denotes	mice
T10429	10119-10121	IN	denotes	in
T10430	10122-10128	NN	denotes	129ola
T10431	10129-10139	NN	denotes	background
T10432	10140-10144	VBD	denotes	were
T10428	10145-10149	VBN	denotes	kept
T10433	10150-10152	IN	denotes	in
T10434	10153-10156	NN	denotes	dim
T10436	10157-10163	JJ	denotes	cyclic
T10435	10164-10169	NN	denotes	light
T10437	10170-10171	-LRB-	denotes	(
T10439	10171-10173	CD	denotes	10
T10438	10174-10177	NN	denotes	lux
T10440	10177-10178	-RRB-	denotes	)
T10441	10179-10182	IN	denotes	for
T10442	10183-10184	CD	denotes	2
T10443	10185-10187	NNS	denotes	wk
T10444	10188-10194	IN	denotes	before
T10446	10195-10215	JJ	denotes	electroretinographic
T10447	10216-10225	NNS	denotes	responses
T10448	10226-10230	VBD	denotes	were
T10445	10231-10239	VBN	denotes	recorded
T10449	10239-10240	.	denotes	.
T10450	10240-10334	sentence	denotes	The mice were anesthetized intraperitoneally with ketamine (80 mg/kg) and xylazine (4 mg/kg).
T10451	10241-10244	DT	denotes	The
T10452	10245-10249	NNS	denotes	mice
T10454	10250-10254	VBD	denotes	were
T10453	10255-10267	VBN	denotes	anesthetized
T10455	10268-10285	RB	denotes	intraperitoneally
T10456	10286-10290	IN	denotes	with
T10457	10291-10299	NN	denotes	ketamine
T10458	10300-10301	-LRB-	denotes	(
T10460	10301-10303	CD	denotes	80
T10459	10304-10306	NNS	denotes	mg
T10461	10306-10307	SYM	denotes	/
T10462	10307-10309	NN	denotes	kg
T10463	10309-10310	-RRB-	denotes	)
T10464	10311-10314	CC	denotes	and
T10465	10315-10323	NN	denotes	xylazine
T10466	10324-10325	-LRB-	denotes	(
T10468	10325-10326	CD	denotes	4
T10467	10327-10329	NNS	denotes	mg
T10469	10329-10330	SYM	denotes	/
T10470	10330-10332	NN	denotes	kg
T10471	10332-10333	-RRB-	denotes	)
T10472	10333-10334	.	denotes	.
T10473	10334-10426	sentence	denotes	Body temperature was maintained with a heating pad at 37.5 ± 1 °C throughout the recording.
T10474	10335-10339	NN	denotes	Body
T10475	10340-10351	NN	denotes	temperature
T10477	10352-10355	VBD	denotes	was
T10476	10356-10366	VBN	denotes	maintained
T10478	10367-10371	IN	denotes	with
T10479	10372-10373	DT	denotes	a
T10481	10374-10381	NN	denotes	heating
T10480	10382-10385	NN	denotes	pad
T10482	10386-10388	IN	denotes	at
T10483	10389-10393	CD	denotes	37.5
T10485	10394-10395	SYM	denotes	±
T10484	10396-10397	CD	denotes	1
T10486	10398-10400	NN	denotes	°C
T10487	10401-10411	IN	denotes	throughout
T10488	10412-10415	DT	denotes	the
T10489	10416-10425	NN	denotes	recording
T10490	10425-10426	.	denotes	.
T10491	10426-10550	sentence	denotes	The pupils were fully dilated with 0.5% tropicamide and 2.5% phenylephrine HCl, and both eyes were recorded simultaneously.
T10492	10427-10430	DT	denotes	The
T10493	10431-10437	NNS	denotes	pupils
T10495	10438-10442	VBD	denotes	were
T10496	10443-10448	RB	denotes	fully
T10494	10449-10456	VBN	denotes	dilated
T10497	10457-10461	IN	denotes	with
T10498	10462-10465	CD	denotes	0.5
T10499	10465-10466	NN	denotes	%
T10500	10467-10478	NN	denotes	tropicamide
T10501	10479-10482	CC	denotes	and
T10502	10483-10486	CD	denotes	2.5
T10503	10486-10487	NN	denotes	%
T10505	10488-10501	NN	denotes	phenylephrine
T10504	10502-10505	NN	denotes	HCl
T10506	10505-10507	,	denotes	,
T10507	10507-10510	CC	denotes	and
T10508	10511-10515	DT	denotes	both
T10509	10516-10520	NNS	denotes	eyes
T10511	10521-10525	VBD	denotes	were
T10510	10526-10534	VBN	denotes	recorded
T10512	10535-10549	RB	denotes	simultaneously
T10513	10549-10550	.	denotes	.
T10514	10550-10667	sentence	denotes	Corneal gold-wire loops were used as the active electrodes under 0.5% proparacaine hydrochloride topical anesthesia.
T10515	10551-10558	JJ	denotes	Corneal
T10517	10559-10563	JJ	denotes	gold
T10519	10563-10564	HYPH	denotes	-
T10518	10564-10568	NN	denotes	wire
T10516	10569-10574	NNS	denotes	loops
T10521	10575-10579	VBD	denotes	were
T10520	10580-10584	VBN	denotes	used
T10522	10585-10587	IN	denotes	as
T10523	10588-10591	DT	denotes	the
T10525	10592-10598	JJ	denotes	active
T10524	10599-10609	NNS	denotes	electrodes
T10526	10610-10615	IN	denotes	under
T10527	10616-10619	CD	denotes	0.5
T10528	10619-10620	NN	denotes	%
T10530	10621-10633	NN	denotes	proparacaine
T10529	10634-10647	NN	denotes	hydrochloride
T10532	10648-10655	JJ	denotes	topical
T10531	10656-10666	NN	denotes	anesthesia
T10533	10666-10667	.	denotes	.
T10534	10667-10757	sentence	denotes	Gold-wire electrodes placed on the sclera near the limbus served as reference electrodes.
T10535	10668-10672	JJ	denotes	Gold
T10537	10672-10673	HYPH	denotes	-
T10536	10673-10677	NN	denotes	wire
T10538	10678-10688	NNS	denotes	electrodes
T10540	10689-10695	VBN	denotes	placed
T10541	10696-10698	IN	denotes	on
T10542	10699-10702	DT	denotes	the
T10543	10703-10709	NN	denotes	sclera
T10544	10710-10714	IN	denotes	near
T10545	10715-10718	DT	denotes	the
T10546	10719-10725	NN	denotes	limbus
T10539	10726-10732	VBD	denotes	served
T10547	10733-10735	IN	denotes	as
T10548	10736-10745	NN	denotes	reference
T10549	10746-10756	NNS	denotes	electrodes
T10550	10756-10757	.	denotes	.
T10551	10757-10796	sentence	denotes	A ground wire was attached to the ear.
T10552	10758-10759	DT	denotes	A
T10554	10760-10766	NN	denotes	ground
T10553	10767-10771	NN	denotes	wire
T10556	10772-10775	VBD	denotes	was
T10555	10776-10784	VBN	denotes	attached
T10557	10785-10787	IN	denotes	to
T10558	10788-10791	DT	denotes	the
T10559	10792-10795	NN	denotes	ear
T10560	10795-10796	.	denotes	.
T10561	10796-10941	sentence	denotes	Scotopic responses were recorded from threshold to 9 log units of intensity above threshold, amplified, and filtered (5,000 gain, 0.1–1,000 Hz).
T10562	10797-10805	JJ	denotes	Scotopic
T10563	10806-10815	NNS	denotes	responses
T10565	10816-10820	VBD	denotes	were
T10564	10821-10829	VBN	denotes	recorded
T10566	10830-10834	IN	denotes	from
T10567	10835-10844	NN	denotes	threshold
T10568	10845-10847	IN	denotes	to
T10569	10848-10849	CD	denotes	9
T10571	10850-10853	NN	denotes	log
T10570	10854-10859	NNS	denotes	units
T10572	10860-10862	IN	denotes	of
T10573	10863-10872	NN	denotes	intensity
T10574	10873-10878	IN	denotes	above
T10575	10879-10888	NN	denotes	threshold
T10576	10888-10890	,	denotes	,
T10577	10890-10899	VBN	denotes	amplified
T10578	10899-10901	,	denotes	,
T10579	10901-10904	CC	denotes	and
T10580	10905-10913	VBN	denotes	filtered
T10581	10914-10915	-LRB-	denotes	(
T10583	10915-10920	CD	denotes	5,000
T10584	10921-10925	NN	denotes	gain
T10585	10925-10927	,	denotes	,
T10586	10927-10930	CD	denotes	0.1
T10588	10930-10931	SYM	denotes	–
T10587	10931-10936	CD	denotes	1,000
T10582	10937-10939	NN	denotes	Hz
T10589	10939-10940	-RRB-	denotes	)
T10590	10940-10941	.	denotes	.
T10591	10941-11029	sentence	denotes	For photopic recordings background light at 34 cd/m2 was used to suppress rod function.
T10592	10942-10945	IN	denotes	For
T10594	10946-10954	JJ	denotes	photopic
T10595	10955-10965	NNS	denotes	recordings
T10596	10966-10976	NN	denotes	background
T10597	10977-10982	NN	denotes	light
T10598	10983-10985	IN	denotes	at
T10599	10986-10988	CD	denotes	34
T10600	10989-10991	NNS	denotes	cd
T10601	10991-10992	SYM	denotes	/
T10602	10992-10994	NN	denotes	m2
T10603	10995-10998	VBD	denotes	was
T10593	10999-11003	VBN	denotes	used
T10604	11004-11006	TO	denotes	to
T10605	11007-11015	VB	denotes	suppress
T10606	11016-11019	NN	denotes	rod
T10607	11020-11028	NN	denotes	function
T10608	11028-11029	.	denotes	.
R5072	T7787	T7788	nmod	Yeast,screening
R5073	T7789	T7790	nummod	two,hybrid
R5074	T7790	T7788	compound	hybrid,screening
R5075	T7791	T7790	punct	-,hybrid
R5076	T7792	T7788	cc	and,screening
R5077	T7793	T7788	conj	assays,screening
R5078	T7794	T7788	punct	.,screening
R5079	T7796	T7797	det	The,LD
R5080	T7797	T7798	nsubjpass	LD,subcloned
R5081	T7799	T7797	prep	of,LD
R5082	T7800	T7801	amod	human,RanBP2
R5083	T7801	T7799	pobj	RanBP2,of
R5084	T7802	T7803	punct	(,62
R5085	T7803	T7797	parataxis	62,LD
R5086	T7804	T7803	nmod	residues,62
R5087	T7805	T7806	punct	–,711
R5088	T7806	T7803	prep	711,62
R5089	T7807	T7803	punct	),62
R5090	T7808	T7798	auxpass	was,subcloned
R5091	T7809	T7798	prep	in,subcloned
R5092	T7810	T7809	punct	-,in
R5093	T7811	T7809	pobj	frame,in
R5094	T7812	T7798	prep	into,subcloned
R5095	T7813	T7814	det	the,domain
R5096	T7814	T7812	pobj	domain,into
R5097	T7815	T7814	compound	GAL4,domain
R5098	T7816	T7817	compound	DNA,binding
R5099	T7817	T7814	compound	binding,domain
R5100	T7818	T7817	punct	-,binding
R5101	T7819	T7814	prep	of,domain
R5102	T7820	T7821	compound	bait,vector
R5103	T7821	T7819	pobj	vector,of
R5104	T7822	T7821	punct	", ",vector
R5105	T7823	T7821	conj	pBUTE,vector
R5106	T7824	T7823	punct	(,pBUTE
R5107	T7825	T7826	det	a,version
R5108	T7826	T7823	appos	version,pBUTE
R5109	T7827	T7828	npadvmod	kanamycin,resistant
R5110	T7828	T7826	amod	resistant,version
R5111	T7829	T7828	punct	-,resistant
R5112	T7830	T7826	prep	of,version
R5113	T7831	T7832	compound	GAL4,vector
R5114	T7832	T7830	pobj	vector,of
R5115	T7833	T7832	compound	bait,vector
R5116	T7834	T7832	appos	pGBDUC1,vector
R5117	T7835	T7823	punct	),pBUTE
R5118	T7836	T7837	punct	[,62
R5119	T7837	T7823	parataxis	62,pBUTE
R5120	T7838	T7837	punct	],62
R5121	T7839	T7823	cc	and,pBUTE
R5122	T7840	T7841	compound	HybriZAP,vector
R5123	T7841	T7823	conj	vector,pBUTE
R5124	T7842	T7843	compound	pBD,GAL4
R5125	T7843	T7841	compound	GAL4,vector
R5126	T7844	T7843	punct	-,GAL4
R5127	T7845	T7846	punct	(,Stratagene
R5128	T7846	T7841	parataxis	Stratagene,vector
R5129	T7847	T7846	punct	", ",Stratagene
R5130	T7848	T7849	compound	La,Jolla
R5131	T7849	T7846	npadvmod	Jolla,Stratagene
R5132	T7850	T7846	punct	", ",Stratagene
R5133	T7851	T7846	npadvmod	California,Stratagene
R5134	T7852	T7846	punct	", ",Stratagene
R5135	T7853	T7854	compound	United,States
R5136	T7854	T7846	npadvmod	States,Stratagene
R5137	T7855	T7846	punct	),Stratagene
R5138	T7856	T7798	punct	.,subcloned
R5139	T7858	T7859	det	The,former
R5140	T7859	T7860	nsubjpass	former,used
R5141	T7861	T7860	auxpass	was,used
R5142	T7862	T7863	aux	to,screen
R5143	T7863	T7860	advcl	screen,used
R5144	T7864	T7865	punct	~,million
R5145	T7865	T7867	nummod	million,clones
R5146	T7866	T7865	compound	18,million
R5147	T7867	T7863	dobj	clones,screen
R5148	T7868	T7867	prep	via,clones
R5149	T7869	T7868	pobj	mating,via
R5150	T7870	T7867	prep	from,clones
R5151	T7871	T7872	nmod	murine,libraries
R5152	T7872	T7870	pobj	libraries,from
R5153	T7873	T7874	quantmod	9,10
R5154	T7874	T7877	nummod	10,d
R5155	T7875	T7874	punct	-,10
R5156	T7876	T7874	quantmod	to,10
R5157	T7877	T7879	npadvmod	d,old
R5158	T7878	T7874	punct	-,10
R5159	T7879	T7872	amod	old,libraries
R5160	T7880	T7879	punct	-,old
R5161	T7881	T7872	nmod	embryo,libraries
R5162	T7882	T7881	cc	and,embryo
R5163	T7883	T7881	conj	brain,embryo
R5164	T7884	T7872	compound	cDNA,libraries
R5165	T7885	T7872	prep	at,libraries
R5166	T7886	T7887	det	the,Facility
R5167	T7887	T7885	pobj	Facility,at
R5168	T7888	T7887	compound	Molecular,Facility
R5169	T7889	T7887	compound	Interaction,Facility
R5170	T7890	T7887	punct	(,Facility
R5171	T7891	T7887	appos	MIF,Facility
R5172	T7892	T7887	punct	),Facility
R5173	T7893	T7887	punct	", ",Facility
R5174	T7894	T7887	npadvmod	University,Facility
R5175	T7895	T7894	prep	of,University
R5176	T7896	T7895	pobj	Wisconsin,of
R5177	T7897	T7894	punct	", ",University
R5178	T7898	T7894	npadvmod	Madison,University
R5179	T7899	T7894	punct	", ",University
R5180	T7900	T7894	npadvmod	Wisconsin,University
R5181	T7901	T7860	punct	.,used
R5182	T7903	T7904	det	The,latter
R5183	T7904	T7905	nsubjpass	latter,used
R5184	T7906	T7905	auxpass	was,used
R5185	T7907	T7908	aux	to,screen
R5186	T7908	T7905	advcl	screen,used
R5187	T7909	T7908	prep	via,screen
R5188	T7910	T7909	pobj	transformation,via
R5189	T7911	T7910	prep	of,transformation
R5190	T7912	T7913	punct	~,million
R5191	T7913	T7915	nummod	million,clones
R5192	T7914	T7913	compound	5,million
R5193	T7915	T7911	pobj	clones,of
R5194	T7916	T7915	prep	from,clones
R5195	T7917	T7918	amod	bovine,libraries
R5196	T7918	T7916	pobj	libraries,from
R5197	T7919	T7918	amod	retinal,libraries
R5198	T7920	T7918	compound	cDNA,libraries
R5199	T7921	T7922	punct	[,32
R5200	T7922	T7908	parataxis	32,screen
R5201	T7923	T7922	punct	],32
R5202	T7924	T7905	punct	.,used
R5203	T7926	T7927	det	The,screens
R5204	T7927	T7928	nsubj	screens,generated
R5205	T7929	T7930	nummod	six,clones
R5206	T7930	T7928	dobj	clones,generated
R5207	T7931	T7928	punct	.,generated
R5208	T7933	T7934	nummod	One,clones
R5209	T7934	T7940	nsubjpass	clones,isolated
R5210	T7935	T7933	cc	and,One
R5211	T7936	T7933	conj	two,One
R5212	T7937	T7934	nmod	in,clones
R5213	T7938	T7937	punct	-,in
R5214	T7939	T7937	pobj	frame,in
R5215	T7941	T7940	auxpass	were,isolated
R5216	T7942	T7940	advmod	independently,isolated
R5217	T7943	T7940	prep	from,isolated
R5218	T7944	T7945	det	the,libraries
R5219	T7945	T7943	pobj	libraries,from
R5220	T7946	T7945	amod	embryonic,libraries
R5221	T7947	T7946	cc	and,embryonic
R5222	T7948	T7946	conj	adult,embryonic
R5223	T7949	T7945	compound	brain,libraries
R5224	T7950	T7940	punct	", ",isolated
R5225	T7951	T7940	advmod	respectively,isolated
R5226	T7952	T7940	punct	", ",isolated
R5227	T7953	T7940	cc	and,isolated
R5228	T7954	T7955	det	the,interactions
R5229	T7955	T7956	nsubjpass	interactions,validated
R5230	T7956	T7940	conj	validated,isolated
R5231	T7957	T7956	auxpass	were,validated
R5232	T7958	T7956	punct	.,validated
R5233	T7960	T7961	det	The,clones
R5234	T7961	T7963	nsubj	clones,encoded
R5235	T7962	T7961	nummod	three,clones
R5236	T7964	T7963	dobj	Cox11,encoded
R5237	T7965	T7963	punct	.,encoded
R5238	T7967	T7968	nsubjpass	Interactions,quantified
R5239	T7969	T7967	prep	between,Interactions
R5240	T7970	T7969	pobj	Cox11,between
R5241	T7971	T7970	punct	", ",Cox11
R5242	T7972	T7970	conj	LD,Cox11
R5243	T7973	T7972	punct	", ",LD
R5244	T7974	T7972	cc	and,LD
R5245	T7975	T7972	conj	subdomains,LD
R5246	T7976	T7975	advmod	thereof,subdomains
R5247	T7977	T7968	auxpass	were,quantified
R5248	T7978	T7968	prep	by,quantified
R5249	T7979	T7980	amod	liquid,galactosidase
R5250	T7980	T7978	pobj	galactosidase,by
R5251	T7981	T7980	compound	β,galactosidase
R5252	T7982	T7980	punct	-,galactosidase
R5253	T7983	T7984	punct	(,Biosystems
R5254	T7984	T7980	parataxis	Biosystems,galactosidase
R5255	T7985	T7984	compound	Applied,Biosystems
R5256	T7986	T7984	punct	", ",Biosystems
R5257	T7987	T7988	compound	Foster,City
R5258	T7988	T7984	npadvmod	City,Biosystems
R5259	T7989	T7984	punct	", ",Biosystems
R5260	T7990	T7984	npadvmod	California,Biosystems
R5261	T7991	T7984	punct	", ",Biosystems
R5262	T7992	T7993	compound	United,States
R5263	T7993	T7984	npadvmod	States,Biosystems
R5264	T7994	T7984	punct	),Biosystems
R5265	T7995	T7980	cc	and,galactosidase
R5266	T7996	T7997	compound	growth,assays
R5267	T7997	T7980	conj	assays,galactosidase
R5268	T7998	T7999	punct	[,63
R5269	T7999	T7968	parataxis	63,quantified
R5270	T8000	T7999	punct	],63
R5271	T8001	T7968	punct	.,quantified
R5272	T8003	T8004	det	The,speed
R5273	T8004	T8008	nsubjpass	speed,determined
R5274	T8005	T8004	nmod	maximum,speed
R5275	T8006	T8004	amod	specific,speed
R5276	T8007	T8004	compound	growth,speed
R5277	T8009	T8004	punct	(,speed
R5278	T8010	T8004	appos	μmax,speed
R5279	T8011	T8008	punct	),determined
R5280	T8012	T8008	auxpass	was,determined
R5281	T8013	T8008	prep	by,determined
R5282	T8014	T8013	pcomp	calculating,by
R5283	T8015	T8016	nsubj	μmax,x t
R5284	T8016	T8014	ccomp	x t,calculating
R5285	T8017	T8016	punct	=,x t
R5286	T8018	T8016	punct	(,x t
R5287	T8019	T8016	nmod	ln,x t
R5288	T8020	T8016	punct	(,x t
R5289	T8021	T8016	punct	),x t
R5290	T8022	T8016	punct	−,x t
R5291	T8023	T8024	nmod	ln,x 0
R5292	T8024	T8016	appos	x 0,x t
R5293	T8025	T8024	punct	(,x 0
R5294	T8026	T8016	punct	),x t
R5295	T8027	T8016	punct	),x t
R5296	T8028	T8016	punct	/,x t
R5297	T8029	T8016	appos	t,x t
R5298	T8030	T8016	punct	", ",x t
R5299	T8031	T8032	advmod	where,is
R5300	T8032	T8016	advcl	is,x t
R5301	T8033	T8032	nsubj	x t,is
R5302	T8034	T8035	det	the,OD600
R5303	T8035	T8032	attr	OD600,is
R5304	T8036	T8035	prep	of,OD600
R5305	T8037	T8038	det	the,culture
R5306	T8038	T8036	pobj	culture,of
R5307	T8039	T8035	prep	at,OD600
R5308	T8040	T8041	nsubj	t,t
R5309	T8041	T8039	pcomp	t,at
R5310	T8042	T8041	punct	=,t
R5311	T8043	T8032	punct	", ",is
R5312	T8044	T8045	nsubj	x 0,is
R5313	T8045	T8032	conj	is,is
R5314	T8046	T8047	det	the,OD600
R5315	T8047	T8045	attr	OD600,is
R5316	T8048	T8045	prep	at,is
R5317	T8049	T8050	nsubj	t,0
R5318	T8050	T8048	pcomp	0,at
R5319	T8051	T8050	punct	=,0
R5320	T8052	T8045	cc	and,is
R5321	T8053	T8054	nsubj	t,is
R5322	T8054	T8045	conj	is,is
R5323	T8055	T8056	det	the,time
R5324	T8056	T8054	attr	time,is
R5325	T8057	T8056	prep	between,time
R5326	T8058	T8057	pobj	x 0,between
R5327	T8059	T8058	cc	and,x 0
R5328	T8060	T8058	conj	x t,x 0
R5329	T8061	T8008	punct	.,determined
R5330	T8063	T8064	nsubjpass	Assays,performed
R5331	T8065	T8064	auxpass	were,performed
R5332	T8066	T8064	prep	with,performed
R5333	T8067	T8068	nummod	three,clones
R5334	T8068	T8066	pobj	clones,with
R5335	T8069	T8068	amod	independent,clones
R5336	T8070	T8068	cc	and,clones
R5337	T8071	T8072	nummod	three,samples
R5338	T8072	T8068	conj	samples,clones
R5339	T8073	T8072	prep	of,samples
R5340	T8074	T8075	det	each,clone
R5341	T8075	T8073	pobj	clone,of
R5342	T8076	T8064	punct	", ",performed
R5343	T8077	T8078	det	the,results
R5344	T8078	T8079	nsubjpass	results,averaged
R5345	T8079	T8064	conj	averaged,performed
R5346	T8080	T8079	auxpass	were,averaged
R5347	T8081	T8079	punct	", ",averaged
R5348	T8082	T8079	cc	and,averaged
R5349	T8083	T8084	det	the,deviations
R5350	T8084	T8086	nsubj	deviations,calculated
R5351	T8085	T8084	amod	standard,deviations
R5352	T8086	T8079	conj	calculated,averaged
R5353	T8087	T8086	punct	.,calculated
R5354	T8119	T8120	npadvmod	Site,directed
R5355	T8120	T8122	amod	directed,mutagenesis
R5356	T8121	T8120	punct	-,directed
R5357	T8123	T8120	cc	and,directed
R5358	T8124	T8120	conj	deletion,directed
R5359	T8125	T8122	cc	and,mutagenesis
R5360	T8126	T8127	compound	plasmid,construction
R5361	T8127	T8122	conj	construction,mutagenesis
R5362	T8128	T8127	punct	.,construction
R5363	T8130	T8131	compound	Deletion,mutagenesis
R5364	T8131	T8132	nsubjpass	mutagenesis,carried
R5365	T8133	T8132	auxpass	was,carried
R5366	T8134	T8132	prt	out,carried
R5367	T8135	T8132	prep	with,carried
R5368	T8136	T8135	pobj	pairs,with
R5369	T8137	T8136	prep	of,pairs
R5370	T8138	T8137	pobj	primers,of
R5371	T8139	T8138	prep	against,primers
R5372	T8140	T8139	pobj	domains,against
R5373	T8141	T8140	prep	of,domains
R5374	T8142	T8141	pobj	interest,of
R5375	T8143	T8140	acl	described,domains
R5376	T8144	T8143	prep	in,described
R5377	T8145	T8146	det	the,legends
R5378	T8146	T8144	pobj	legends,in
R5379	T8147	T8146	compound	figure,legends
R5380	T8148	T8132	punct	.,carried
R5381	T8150	T8151	compound	PCR,products
R5382	T8151	T8152	nsubjpass	products,subcloned
R5383	T8153	T8152	auxpass	were,subcloned
R5384	T8154	T8152	prep	into,subcloned
R5385	T8155	T8156	compound	pGEX,KG
R5386	T8156	T8154	pobj	KG,into
R5387	T8157	T8156	punct	-,KG
R5388	T8158	T8159	punct	[,64
R5389	T8159	T8156	parataxis	64,KG
R5390	T8160	T8159	punct	],64
R5391	T8161	T8156	punct	", ",KG
R5392	T8162	T8163	compound	HybriZAP,vector
R5393	T8163	T8156	conj	vector,KG
R5394	T8164	T8165	compound	pBD,GAL4
R5395	T8165	T8163	compound	GAL4,vector
R5396	T8166	T8165	punct	-,GAL4
R5397	T8167	T8168	punct	(,Stratagene
R5398	T8168	T8163	parataxis	Stratagene,vector
R5399	T8169	T8168	punct	),Stratagene
R5400	T8170	T8163	punct	", ",vector
R5401	T8171	T8163	cc	and,vector
R5402	T8172	T8173	nmod	pBUTE,vectors
R5403	T8173	T8163	conj	vectors,vector
R5404	T8174	T8175	punct	[,62
R5405	T8175	T8172	parataxis	62,pBUTE
R5406	T8176	T8175	punct	],62
R5407	T8177	T8152	punct	.,subcloned
R5408	T8179	T8180	compound	GST,LDZIP
R5409	T8180	T8182	nsubj	LDZIP,comprised
R5410	T8181	T8180	punct	-,LDZIP
R5411	T8183	T8180	advmod	alone,LDZIP
R5412	T8184	T8185	nmod	residues,447
R5413	T8185	T8182	dobj	447,comprised
R5414	T8186	T8187	punct	–,483
R5415	T8187	T8185	prep	483,447
R5416	T8188	T8185	prep	of,447
R5417	T8189	T8190	amod	human,RanBP2
R5418	T8190	T8188	pobj	RanBP2,of
R5419	T8191	T8182	punct	.,comprised
R5420	T8257	T8258	nmod	GST,assays
R5421	T8259	T8258	amod	pull,assays
R5422	T8260	T8259	punct	-,pull
R5423	T8261	T8259	prt	down,pull
R5424	T8262	T8259	cc	and,pull
R5425	T8263	T8259	conj	immunoprecipitation,pull
R5426	T8264	T8258	punct	.,assays
R5427	T8266	T8267	npadvmod	CHAPS,solubilized
R5428	T8267	T8269	amod	solubilized,extracts
R5429	T8268	T8267	punct	-,solubilized
R5430	T8269	T8271	nsubjpass	extracts,prepared
R5431	T8270	T8269	amod	retinal,extracts
R5432	T8272	T8269	punct	", ",extracts
R5433	T8273	T8269	conj	expression,extracts
R5434	T8274	T8273	punct	", ",expression
R5435	T8275	T8273	cc	and,expression
R5436	T8276	T8273	conj	purification,expression
R5437	T8277	T8276	prep	of,purification
R5438	T8278	T8279	npadvmod	GST,fused
R5439	T8279	T8281	amod	fused,constructs
R5440	T8280	T8279	punct	-,fused
R5441	T8281	T8277	pobj	constructs,of
R5442	T8282	T8271	auxpass	were,prepared
R5443	T8283	T8284	mark	as,described
R5444	T8284	T8271	advcl	described,prepared
R5445	T8285	T8284	advmod	previously,described
R5446	T8286	T8287	punct	[,65
R5447	T8287	T8271	parataxis	65,prepared
R5448	T8288	T8287	punct	],65
R5449	T8289	T8271	punct	.,prepared
R5450	T8291	T8292	nmod	GST,assays
R5451	T8292	T8296	nsubjpass	assays,carried
R5452	T8293	T8292	amod	pull,assays
R5453	T8294	T8293	punct	-,pull
R5454	T8295	T8293	prt	down,pull
R5455	T8297	T8296	auxpass	were,carried
R5456	T8298	T8296	prt	out,carried
R5457	T8299	T8296	prep	with,carried
R5458	T8300	T8301	quantmod	0.5,2.2
R5459	T8301	T8303	nummod	2.2,μM
R5460	T8302	T8301	punct	–,2.2
R5461	T8303	T8299	pobj	μM,with
R5462	T8304	T8303	prep	of,μM
R5463	T8305	T8306	npadvmod	GST,fused
R5464	T8306	T8308	amod	fused,proteins
R5465	T8307	T8306	punct	-,fused
R5466	T8308	T8304	pobj	proteins,of
R5467	T8309	T8310	punct	[,65
R5468	T8310	T8296	parataxis	65,carried
R5469	T8311	T8310	punct	],65
R5470	T8312	T8296	punct	.,carried
R5471	T8314	T8315	nsubjpass	Co-precipitates,resolved
R5472	T8316	T8315	auxpass	were,resolved
R5473	T8317	T8315	prep	on,resolved
R5474	T8318	T8319	compound	SDS,PAGE
R5475	T8319	T8317	pobj	PAGE,on
R5476	T8320	T8319	punct	-,PAGE
R5477	T8321	T8315	cc	and,resolved
R5478	T8322	T8315	conj	analyzed,resolved
R5479	T8323	T8322	prep	by,analyzed
R5480	T8324	T8325	compound	Western,blot
R5481	T8325	T8323	pobj	blot,by
R5482	T8326	T8322	prep	with,analyzed
R5483	T8327	T8326	pobj	antibodies,with
R5484	T8328	T8327	acl	described,antibodies
R5485	T8329	T8328	prep	in,described
R5486	T8330	T8331	det	the,section
R5487	T8331	T8329	pobj	section,in
R5488	T8332	T8331	compound	Results,section
R5489	T8333	T8315	punct	.,resolved
R5490	T8335	T8336	amod	Unfolded,Cox11
R5491	T8336	T8340	nsubjpass	Cox11,generated
R5492	T8337	T8335	cc	and,Unfolded
R5493	T8338	T8339	advmod	partially,denatured
R5494	T8339	T8335	conj	denatured,Unfolded
R5495	T8341	T8340	auxpass	were,generated
R5496	T8342	T8340	prep	by,generated
R5497	T8343	T8342	pcomp	incubating,by
R5498	T8344	T8345	amod	recombinant,Cox11
R5499	T8345	T8343	dobj	Cox11,incubating
R5500	T8346	T8344	cc	and,recombinant
R5501	T8347	T8344	conj	native,recombinant
R5502	T8348	T8340	advmod	overnight,generated
R5503	T8349	T8340	prep	with,generated
R5504	T8350	T8351	nummod	5.6,M
R5505	T8351	T8349	pobj	M,with
R5506	T8352	T8350	cc	and,5.6
R5507	T8353	T8350	conj	7,5.6
R5508	T8354	T8355	compound	guanidine,hydrochloride
R5509	T8355	T8351	appos	hydrochloride,M
R5510	T8356	T8355	cc	and,hydrochloride
R5511	T8357	T8355	conj	urea,hydrochloride
R5512	T8358	T8340	punct	", ",generated
R5513	T8359	T8340	advmod	respectively,generated
R5514	T8360	T8340	punct	.,generated
R5515	T8362	T8363	det	The,conformers
R5516	T8363	T8365	nsubjpass	conformers,diluted
R5517	T8364	T8363	compound	Cox11,conformers
R5518	T8366	T8365	auxpass	were,diluted
R5519	T8367	T8365	advmod	then,diluted
R5520	T8368	T8369	punct	~,20-fold
R5521	T8369	T8365	advmod	20-fold,diluted
R5522	T8370	T8365	prep	in,diluted
R5523	T8371	T8372	compound	CHAPS,binding
R5524	T8372	T8374	compound	binding,buffer
R5525	T8373	T8372	punct	-,binding
R5526	T8374	T8370	pobj	buffer,in
R5527	T8375	T8374	acl	containing,buffer
R5528	T8376	T8377	compound	GST,LD
R5529	T8377	T8375	dobj	LD,containing
R5530	T8378	T8377	punct	-,LD
R5531	T8379	T8365	punct	.,diluted
R5532	T8381	T8382	compound	Immunoprecipitation,assays
R5533	T8382	T8383	nsubjpass	assays,performed
R5534	T8384	T8382	prep	with,assays
R5535	T8385	T8386	nummod	5,μg
R5536	T8386	T8384	pobj	μg,with
R5537	T8387	T8386	prep	of,μg
R5538	T8388	T8387	pobj	antibody,of
R5539	T8389	T8382	cc	and,assays
R5540	T8390	T8391	compound	Western,blots
R5541	T8391	T8382	conj	blots,assays
R5542	T8392	T8393	punct	(,ng
R5543	T8393	T8391	parataxis	ng,blots
R5544	T8394	T8395	punct	~,400
R5545	T8395	T8393	nummod	400,ng
R5546	T8396	T8395	quantmod	200,400
R5547	T8397	T8395	punct	–,400
R5548	T8398	T8399	punct	/,ml
R5549	T8399	T8393	prep	ml,ng
R5550	T8400	T8399	prep	of,ml
R5551	T8401	T8400	pobj	antibody,of
R5552	T8402	T8393	punct	),ng
R5553	T8403	T8383	auxpass	were,performed
R5554	T8404	T8405	advmod	exactly,described
R5555	T8405	T8383	advcl	described,performed
R5556	T8406	T8405	mark	as,described
R5557	T8407	T8405	advmod	previously,described
R5558	T8408	T8409	punct	[,13
R5559	T8409	T8405	parataxis	13,described
R5560	T8410	T8409	punct	],13
R5561	T8411	T8383	punct	.,performed
R5562	T8483	T8484	nmod	Hexokinase,assay
R5563	T8485	T8483	nummod	I,Hexokinase
R5564	T8486	T8484	punct	.,assay
R5565	T8488	T8489	nmod	Hexokinase,activity
R5566	T8489	T8491	nsubjpass	activity,determined
R5567	T8490	T8488	nummod	I,Hexokinase
R5568	T8492	T8491	auxpass	was,determined
R5569	T8493	T8491	advmod	spectrophotometrically,determined
R5570	T8494	T8491	prep	at,determined
R5571	T8495	T8496	nummod	25,°C
R5572	T8496	T8494	pobj	°C,at
R5573	T8497	T8491	prep	by,determined
R5574	T8498	T8499	det	the,method
R5575	T8499	T8497	pobj	method,by
R5576	T8500	T8499	prep	of,method
R5577	T8501	T8500	pcomp	coupling,of
R5578	T8502	T8503	det	the,production
R5579	T8503	T8501	dobj	production,coupling
R5580	T8504	T8505	nmod	glucose,phosphate
R5581	T8505	T8503	compound	phosphate,production
R5582	T8506	T8505	punct	-,phosphate
R5583	T8507	T8505	nummod	6,phosphate
R5584	T8508	T8505	punct	-,phosphate
R5585	T8509	T8503	prep	via,production
R5586	T8510	T8511	nmod	glucose,phosphate
R5587	T8511	T8515	compound	phosphate,dehydrogenase
R5588	T8512	T8511	punct	-,phosphate
R5589	T8513	T8511	nummod	6,phosphate
R5590	T8514	T8511	punct	-,phosphate
R5591	T8515	T8509	pobj	dehydrogenase,via
R5592	T8516	T8501	prep	with,coupling
R5593	T8517	T8518	det	the,change
R5594	T8518	T8516	pobj	change,with
R5595	T8519	T8518	prep	in,change
R5596	T8520	T8521	det	the,absorbance
R5597	T8521	T8519	pobj	absorbance,in
R5598	T8522	T8521	prep	of,absorbance
R5599	T8523	T8522	pobj	NADPH,of
R5600	T8524	T8518	prep	at,change
R5601	T8525	T8526	nummod	340,nm
R5602	T8526	T8524	pobj	nm,at
R5603	T8527	T8497	cc	and,by
R5604	T8528	T8529	mark	as,described
R5605	T8529	T8497	conj	described,by
R5606	T8530	T8529	agent	by,described
R5607	T8531	T8532	punct	[,66
R5608	T8532	T8530	pobj	66,by
R5609	T8533	T8491	punct	],determined
R5610	T8534	T8491	punct	.,determined
R5611	T8536	T8537	det	The,reaction
R5612	T8537	T8538	nsubjpass	reaction,started
R5613	T8539	T8538	auxpass	was,started
R5614	T8540	T8538	agent	by,started
R5615	T8541	T8542	det	the,addition
R5616	T8542	T8540	pobj	addition,by
R5617	T8543	T8542	prep	of,addition
R5618	T8544	T8545	amod	purified,hexokinase
R5619	T8545	T8543	pobj	hexokinase,of
R5620	T8546	T8545	compound	brain,hexokinase
R5621	T8547	T8545	nummod	I,hexokinase
R5622	T8548	T8549	punct	(,μg
R5623	T8549	T8545	parataxis	μg,hexokinase
R5624	T8550	T8549	nummod	0.24,μg
R5625	T8551	T8549	punct	),μg
R5626	T8552	T8553	punct	(,gift
R5627	T8553	T8545	parataxis	gift,hexokinase
R5628	T8554	T8553	prep	from,gift
R5629	T8555	T8556	compound	J.,Wilson
R5630	T8556	T8554	pobj	Wilson,from
R5631	T8557	T8553	punct	),gift
R5632	T8558	T8542	prep	to,addition
R5633	T8559	T8560	nummod	1.0,ml
R5634	T8560	T8558	pobj	ml,to
R5635	T8561	T8560	prep	of,ml
R5636	T8562	T8563	compound	reaction,mixture
R5637	T8563	T8561	pobj	mixture,of
R5638	T8564	T8560	acl	containing,ml
R5639	T8565	T8566	nummod	0.05,M
R5640	T8566	T8567	compound	M,HCl
R5641	T8567	T8564	dobj	HCl,containing
R5642	T8568	T8567	compound	Tris,HCl
R5643	T8569	T8567	punct	-,HCl
R5644	T8570	T8571	punct	(,pH
R5645	T8571	T8567	parataxis	pH,HCl
R5646	T8572	T8571	nummod	8.5,pH
R5647	T8573	T8571	punct	),pH
R5648	T8574	T8567	punct	", ",HCl
R5649	T8575	T8576	nummod	7.4,mM
R5650	T8576	T8577	compound	mM,MgCl2
R5651	T8577	T8567	conj	MgCl2,HCl
R5652	T8578	T8577	punct	", ",MgCl2
R5653	T8579	T8580	nummod	6.6,mM
R5654	T8580	T8581	compound	mM,ATP
R5655	T8581	T8577	conj	ATP,MgCl2
R5656	T8582	T8581	punct	", ",ATP
R5657	T8583	T8584	nummod	0.65,mM
R5658	T8584	T8585	compound	mM,NADP
R5659	T8585	T8581	conj	NADP,ATP
R5660	T8586	T8585	punct	", ",NADP
R5661	T8587	T8588	nummod	11.1,mM
R5662	T8588	T8589	compound	mM,monothioglycerol
R5663	T8589	T8585	conj	monothioglycerol,NADP
R5664	T8590	T8589	punct	", ",monothioglycerol
R5665	T8591	T8589	cc	and,monothioglycerol
R5666	T8592	T8593	nummod	1,unit
R5667	T8593	T8589	conj	unit,monothioglycerol
R5668	T8594	T8593	prep	of,unit
R5669	T8595	T8596	nmod	glucose,phosphate
R5670	T8596	T8599	compound	phosphate,dehydrogenase
R5671	T8597	T8596	nummod	6,phosphate
R5672	T8598	T8596	punct	-,phosphate
R5673	T8599	T8594	pobj	dehydrogenase,of
R5674	T8600	T8538	punct	.,started
R5675	T8602	T8603	prep	In,incubated
R5676	T8604	T8605	det	the,case
R5677	T8605	T8602	pobj	case,In
R5678	T8606	T8605	prep	of,case
R5679	T8607	T8608	compound	hexokinase,activity
R5680	T8608	T8606	pobj	activity,of
R5681	T8609	T8608	acl	measured,activity
R5682	T8610	T8609	prep	in,measured
R5683	T8611	T8612	det	the,presence
R5684	T8612	T8610	pobj	presence,in
R5685	T8613	T8612	prep	of,presence
R5686	T8614	T8613	pobj	Cox11,of
R5687	T8615	T8614	cc	and,Cox11
R5688	T8616	T8614	conj	LD,Cox11
R5689	T8617	T8603	punct	", ",incubated
R5690	T8618	T8619	det	the,HKI
R5691	T8619	T8603	nsubjpass	HKI,incubated
R5692	T8620	T8619	amod	purified,HKI
R5693	T8621	T8603	auxpass	was,incubated
R5694	T8622	T8603	prep	with,incubated
R5695	T8623	T8624	det	the,proteins
R5696	T8624	T8622	pobj	proteins,with
R5697	T8625	T8624	amod	recombinant,proteins
R5698	T8626	T8603	prep	for,incubated
R5699	T8627	T8628	nummod	15,min
R5700	T8628	T8626	pobj	min,for
R5701	T8629	T8603	prep	at,incubated
R5702	T8630	T8631	nummod	4,°C
R5703	T8631	T8629	pobj	°C,at
R5704	T8632	T8603	prep	before,incubated
R5705	T8633	T8632	pobj	measurement,before
R5706	T8634	T8633	prep	of,measurement
R5707	T8635	T8636	det	the,activity
R5708	T8636	T8634	pobj	activity,of
R5709	T8637	T8603	punct	.,incubated
R5710	T8639	T8640	det	The,data
R5711	T8640	T8641	nsubjpass	data,fitted
R5712	T8642	T8641	auxpass	were,fitted
R5713	T8643	T8641	advmod	directly,fitted
R5714	T8644	T8641	prep	into,fitted
R5715	T8645	T8646	det	the,equation
R5716	T8646	T8644	pobj	equation,into
R5717	T8647	T8648	compound	Michaelis,Menten
R5718	T8648	T8646	compound	Menten,equation
R5719	T8649	T8648	punct	-,Menten
R5720	T8650	T8641	advcl	using,fitted
R5721	T8651	T8650	dobj	SIGMAPLOT,using
R5722	T8652	T8653	punct	(,Science
R5723	T8653	T8651	parataxis	Science,SIGMAPLOT
R5724	T8654	T8653	compound	SPSS,Science
R5725	T8655	T8653	punct	),Science
R5726	T8656	T8641	punct	.,fitted
R5727	T8701	T8700	punct	.,Antibodies
R5728	T8703	T8704	compound	Rabbit,antisera
R5729	T8704	T8705	nsubjpass	antisera,raised
R5730	T8706	T8705	auxpass	were,raised
R5731	T8707	T8705	prep	against,raised
R5732	T8708	T8709	det	the,Cox11
R5733	T8709	T8707	pobj	Cox11,against
R5734	T8710	T8709	amod	recombinant,Cox11
R5735	T8711	T8709	amod	murine,Cox11
R5736	T8712	T8713	punct	(,40
R5737	T8713	T8707	parataxis	40,against
R5738	T8714	T8713	nmod	residues,40
R5739	T8715	T8716	punct	–,275
R5740	T8716	T8713	prep	275,40
R5741	T8717	T8713	punct	),40
R5742	T8718	T8705	cc	and,raised
R5743	T8719	T8720	dep	affinity,purified
R5744	T8720	T8705	conj	purified,raised
R5745	T8721	T8720	punct	-,purified
R5746	T8722	T8720	prep	under,purified
R5747	T8723	T8724	compound	non-denaturing,conditions
R5748	T8724	T8722	pobj	conditions,under
R5749	T8725	T8726	punct	(,Stereogene
R5750	T8726	T8724	parataxis	Stereogene,conditions
R5751	T8727	T8726	punct	),Stereogene
R5752	T8728	T8729	mark	as,described
R5753	T8729	T8720	advcl	described,purified
R5754	T8730	T8729	advmod	previously,described
R5755	T8731	T8732	punct	[,13
R5756	T8732	T8729	parataxis	13,described
R5757	T8733	T8732	punct	],13
R5758	T8734	T8705	punct	.,raised
R5759	T8736	T8737	compound	Cox11,antibodies
R5760	T8737	T8738	nsubjpass	antibodies,used
R5761	T8739	T8738	auxpass	were,used
R5762	T8740	T8738	prep	at,used
R5763	T8741	T8742	nummod	200,ng
R5764	T8742	T8740	pobj	ng,at
R5765	T8743	T8744	punct	/,ml
R5766	T8744	T8742	prep	ml,ng
R5767	T8745	T8738	prep	for,used
R5768	T8746	T8747	compound	Western,analysis
R5769	T8747	T8745	pobj	analysis,for
R5770	T8748	T8738	punct	.,used
R5771	T8750	T8751	det	The,Hsp70
R5772	T8751	T8754	nsubj	Hsp70,was
R5773	T8752	T8751	amod	monoclonal,Hsp70
R5774	T8753	T8751	compound	antibody,Hsp70
R5775	T8755	T8754	prep	from,was
R5776	T8756	T8757	compound	Affinity,Bioreagents
R5777	T8757	T8755	pobj	Bioreagents,from
R5778	T8758	T8759	punct	(,Golden
R5779	T8759	T8757	parataxis	Golden,Bioreagents
R5780	T8760	T8759	punct	", ",Golden
R5781	T8761	T8759	npadvmod	Colorado,Golden
R5782	T8762	T8759	punct	", ",Golden
R5783	T8763	T8764	compound	United,States
R5784	T8764	T8759	npadvmod	States,Golden
R5785	T8765	T8759	punct	),Golden
R5786	T8766	T8754	cc	and,was
R5787	T8767	T8768	nsubjpass	antibodies,described
R5788	T8768	T8754	conj	described,was
R5789	T8769	T8767	prep	against,antibodies
R5790	T8770	T8771	det	the,domains
R5791	T8771	T8769	pobj	domains,against
R5792	T8772	T8771	nmod	KBD,domains
R5793	T8773	T8774	punct	(,JX2
R5794	T8774	T8772	parataxis	JX2,KBD
R5795	T8775	T8774	punct	),JX2
R5796	T8776	T8772	cc	and,KBD
R5797	T8777	T8772	conj	ZnF,KBD
R5798	T8778	T8771	prep	of,domains
R5799	T8779	T8778	pobj	RanBP2,of
R5800	T8780	T8768	aux	have,described
R5801	T8781	T8768	auxpass	been,described
R5802	T8782	T8783	punct	[,16
R5803	T8783	T8768	parataxis	16,described
R5804	T8784	T8783	nummod	13,16
R5805	T8785	T8783	punct	",",16
R5806	T8786	T8783	punct	],16
R5807	T8787	T8768	punct	.,described
R5808	T8789	T8790	amod	Polyclonal,antibodies
R5809	T8790	T8793	nsubjpass	antibodies,provided
R5810	T8791	T8789	cc	and,Polyclonal
R5811	T8792	T8789	conj	monoclonal,Polyclonal
R5812	T8794	T8790	prep	against,antibodies
R5813	T8795	T8794	pobj	Hexokinase,against
R5814	T8796	T8795	nmod	I,Hexokinase
R5815	T8797	T8793	auxpass	were,provided
R5816	T8798	T8793	agent	by,provided
R5817	T8799	T8800	compound	J.,Wilson
R5818	T8800	T8798	pobj	Wilson,by
R5819	T8801	T8793	punct	.,provided
R5820	T8803	T8804	det	The,mAb414
R5821	T8804	T8805	nsubjpass	mAb414,purchased
R5822	T8806	T8805	auxpass	was,purchased
R5823	T8807	T8805	prep	from,purchased
R5824	T8808	T8807	pobj	Abcam,from
R5825	T8809	T8810	punct	(,Cambridge
R5826	T8810	T8808	parataxis	Cambridge,Abcam
R5827	T8811	T8810	punct	", ",Cambridge
R5828	T8812	T8810	npadvmod	Massachusetts,Cambridge
R5829	T8813	T8810	punct	", ",Cambridge
R5830	T8814	T8815	compound	United,States
R5831	T8815	T8810	npadvmod	States,Cambridge
R5832	T8816	T8810	punct	),Cambridge
R5833	T8817	T8805	punct	.,purchased
R5834	T8884	T8883	cc	and,Immunocytochemistry
R5835	T8885	T8883	conj	microscopy,Immunocytochemistry
R5836	T8886	T8885	punct	.,microscopy
R5837	T8888	T8889	compound	Retina,dissections
R5838	T8889	T8890	nsubjpass	dissections,carried
R5839	T8891	T8889	punct	", ",dissections
R5840	T8892	T8893	amod	radial,cryosections
R5841	T8893	T8889	conj	cryosections,dissections
R5842	T8894	T8895	punct	(,μm
R5843	T8895	T8893	parataxis	μm,cryosections
R5844	T8896	T8897	punct	~,6
R5845	T8897	T8895	nummod	6,μm
R5846	T8898	T8895	punct	),μm
R5847	T8899	T8893	punct	", ",cryosections
R5848	T8900	T8893	cc	and,cryosections
R5849	T8901	T8902	compound	immunohistochemistry,procedures
R5850	T8902	T8893	conj	procedures,cryosections
R5851	T8903	T8890	auxpass	were,carried
R5852	T8904	T8890	prt	out,carried
R5853	T8905	T8906	mark	as,described
R5854	T8906	T8890	advcl	described,carried
R5855	T8907	T8906	advmod	elsewhere,described
R5856	T8908	T8909	punct	[,19
R5857	T8909	T8906	parataxis	19,described
R5858	T8910	T8909	punct	],19
R5859	T8911	T8890	punct	.,carried
R5860	T8913	T8914	nsubjpass	Brains,fixed
R5861	T8915	T8913	prep	from,Brains
R5862	T8916	T8917	quantmod	3,5
R5863	T8917	T8920	nummod	5,mo
R5864	T8918	T8917	punct	-,5
R5865	T8919	T8917	quantmod	to,5
R5866	T8920	T8922	npadvmod	mo,old
R5867	T8921	T8920	punct	-,mo
R5868	T8922	T8924	amod	old,mice
R5869	T8923	T8922	punct	-,old
R5870	T8924	T8915	pobj	mice,from
R5871	T8925	T8924	nmod	C57Bl,mice
R5872	T8926	T8925	punct	/,C57Bl
R5873	T8927	T8925	nummod	6,C57Bl
R5874	T8928	T8914	auxpass	were,fixed
R5875	T8929	T8914	advmod	overnight,fixed
R5876	T8930	T8914	prep	in,fixed
R5877	T8931	T8932	nummod	2,%
R5878	T8932	T8933	compound	%,paraformaldehyde
R5879	T8933	T8930	pobj	paraformaldehyde,in
R5880	T8934	T8914	punct	", ",fixed
R5881	T8935	T8914	conj	infused,fixed
R5882	T8936	T8935	prep	with,infused
R5883	T8937	T8938	nummod	30,%
R5884	T8938	T8939	compound	%,sucrose
R5885	T8939	T8936	pobj	sucrose,with
R5886	T8940	T8935	punct	", ",infused
R5887	T8941	T8935	cc	and,infused
R5888	T8942	T8935	conj	processed,infused
R5889	T8943	T8942	prep	for,processed
R5890	T8944	T8943	pobj	cryosectioning,for
R5891	T8945	T8914	punct	.,fixed
R5892	T8947	T8948	amod	Primary,neurons
R5893	T8948	T8950	nsubjpass	neurons,prepared
R5894	T8949	T8948	compound	brain,neurons
R5895	T8951	T8948	cc	and,neurons
R5896	T8952	T8953	amod	glial,cells
R5897	T8953	T8948	conj	cells,neurons
R5898	T8954	T8950	auxpass	were,prepared
R5899	T8955	T8950	prep	from,prepared
R5900	T8956	T8957	det	the,cortex
R5901	T8957	T8955	pobj	cortex,from
R5902	T8958	T8957	amod	cerebral,cortex
R5903	T8959	T8950	punct	.,prepared
R5904	T8961	T8962	nsubjpass	This,macerated
R5905	T8963	T8962	auxpass	was,macerated
R5906	T8964	T8962	prep	in,macerated
R5907	T8965	T8966	det	the,solution
R5908	T8966	T8964	pobj	solution,in
R5909	T8967	T8968	poss	Hank,Salt
R5910	T8968	T8966	compound	Salt,solution
R5911	T8969	T8967	case	's,Hank
R5912	T8970	T8968	compound	Balanced,Salt
R5913	T8971	T8962	cc	and,macerated
R5914	T8972	T8962	conj	triturated,macerated
R5915	T8973	T8962	punct	.,macerated
R5916	T8975	T8976	amod	Primary,cells
R5917	T8976	T8977	nsubjpass	cells,cultured
R5918	T8978	T8977	auxpass	were,cultured
R5919	T8979	T8977	prep	in,cultured
R5920	T8980	T8979	pobj	DMEM,in
R5921	T8981	T8982	punct	(,GIBCO
R5922	T8982	T8980	parataxis	GIBCO,DMEM
R5923	T8983	T8982	punct	", ",GIBCO
R5924	T8984	T8985	compound	San,Diego
R5925	T8985	T8982	npadvmod	Diego,GIBCO
R5926	T8986	T8982	punct	", ",GIBCO
R5927	T8987	T8982	npadvmod	California,GIBCO
R5928	T8988	T8982	punct	", ",GIBCO
R5929	T8989	T8990	compound	United,States
R5930	T8990	T8982	npadvmod	States,GIBCO
R5931	T8991	T8982	punct	),GIBCO
R5932	T8992	T8980	cc	and,DMEM
R5933	T8993	T8994	npadvmod	collagen,coated
R5934	T8994	T8996	amod	coated,dishes
R5935	T8995	T8994	punct	-,coated
R5936	T8996	T8980	conj	dishes,DMEM
R5937	T8997	T8998	nummod	35,mm
R5938	T8998	T8996	compound	mm,dishes
R5939	T8999	T8998	punct	-,mm
R5940	T9000	T9001	compound	glass,bottom
R5941	T9001	T8996	compound	bottom,dishes
R5942	T9002	T8996	compound	culture,dishes
R5943	T9003	T9004	punct	(,Corporation
R5944	T9004	T8996	parataxis	Corporation,dishes
R5945	T9005	T9004	compound	MatTek,Corporation
R5946	T9006	T9004	punct	", ",Corporation
R5947	T9007	T9004	npadvmod	Ashland,Corporation
R5948	T9008	T9004	punct	", ",Corporation
R5949	T9009	T9004	npadvmod	Maine,Corporation
R5950	T9010	T9004	punct	),Corporation
R5951	T9011	T8977	prep	at,cultured
R5952	T9012	T9013	nummod	5,%
R5953	T9013	T9014	compound	%,CO2
R5954	T9014	T9011	pobj	CO2,at
R5955	T9015	T9014	punct	/,CO2
R5956	T9016	T9017	nummod	37,°C
R5957	T9017	T9014	appos	°C,CO2
R5958	T9018	T8977	prep	for,cultured
R5959	T9019	T9020	punct	~,2
R5960	T9020	T9021	nummod	2,d
R5961	T9021	T9018	pobj	d,for
R5962	T9022	T8977	cc	and,cultured
R5963	T9023	T8977	conj	processed,cultured
R5964	T9024	T9023	prep	for,processed
R5965	T9025	T9024	pobj	immunocytochemistry,for
R5966	T9026	T8977	punct	.,cultured
R5967	T9028	T9029	amod	Primary,antibodies
R5968	T9029	T9030	nsubjpass	antibodies,used
R5969	T9031	T9030	auxpass	were,used
R5970	T9032	T9030	prep	at,used
R5971	T9033	T9032	pobj	concentrations,at
R5972	T9034	T9035	punct	~,10
R5973	T9035	T9038	nummod	10,μg
R5974	T9036	T9035	quantmod	2.5,10
R5975	T9037	T9035	punct	–,10
R5976	T9038	T9033	npadvmod	μg,concentrations
R5977	T9039	T9040	punct	/,ml
R5978	T9040	T9038	prep	ml,μg
R5979	T9041	T9030	punct	.,used
R5980	T9043	T9044	npadvmod	Alexa,conjugated
R5981	T9044	T9051	amod	conjugated,antibodies
R5982	T9045	T9043	nummod	488,Alexa
R5983	T9046	T9043	punct	-,Alexa
R5984	T9047	T9043	cc	and,Alexa
R5985	T9048	T9043	conj	Alexa,Alexa
R5986	T9049	T9048	amod	594,Alexa
R5987	T9050	T9044	punct	-,conjugated
R5988	T9051	T9053	nsubjpass	antibodies,used
R5989	T9052	T9051	amod	secondary,antibodies
R5990	T9054	T9055	punct	(,μg
R5991	T9055	T9051	parataxis	μg,antibodies
R5992	T9056	T9055	nummod	2.5,μg
R5993	T9057	T9058	punct	/,ml
R5994	T9058	T9055	prep	ml,μg
R5995	T9059	T9055	punct	),μg
R5996	T9060	T9061	punct	(,Probes
R5997	T9061	T9051	parataxis	Probes,antibodies
R5998	T9062	T9061	compound	Molecular,Probes
R5999	T9063	T9061	punct	),Probes
R6000	T9064	T9053	auxpass	were,used
R6001	T9065	T9053	prep	for,used
R6002	T9066	T9065	pobj	visualization,for
R6003	T9067	T9066	prep	of,visualization
R6004	T9068	T9067	pobj	proteins,of
R6005	T9069	T9053	punct	.,used
R6006	T9071	T9072	nsubjpass	Crossover,found
R6007	T9073	T9071	prep	of,Crossover
R6008	T9074	T9075	amod	fluorescent,probes
R6009	T9075	T9073	pobj	probes,of
R6010	T9076	T9075	punct	", ",probes
R6011	T9077	T9075	conj	background,probes
R6012	T9078	T9077	punct	", ",background
R6013	T9079	T9077	cc	and,background
R6014	T9080	T9077	conj	autofluorescence,background
R6015	T9081	T9072	auxpass	were,found
R6016	T9082	T9083	aux	to,be
R6017	T9083	T9072	xcomp	be,found
R6018	T9084	T9083	acomp	negligible,be
R6019	T9085	T9072	punct	.,found
R6020	T9087	T9088	nsubjpass	Visualization,carried
R6021	T9089	T9087	prep	of,Visualization
R6022	T9090	T9089	pobj	specimens,of
R6023	T9091	T9087	cc	and,Visualization
R6024	T9092	T9087	conj	localization,Visualization
R6025	T9093	T9092	prep	of,localization
R6026	T9094	T9093	pobj	proteins,of
R6027	T9095	T9088	auxpass	were,carried
R6028	T9096	T9088	prt	out,carried
R6029	T9097	T9088	prep	by,carried
R6030	T9098	T9099	amod	wide,field
R6031	T9099	T9101	compound	field,microscopy
R6032	T9100	T9099	punct	-,field
R6033	T9101	T9097	pobj	microscopy,by
R6034	T9102	T9101	compound	epifluorescence,microscopy
R6035	T9103	T9088	prep	on,carried
R6036	T9104	T9105	nmod	Nikon,upright
R6037	T9105	T9103	pobj	upright,on
R6038	T9106	T9107	punct	(,Tokyo
R6039	T9107	T9104	parataxis	Tokyo,Nikon
R6040	T9108	T9107	punct	", ",Tokyo
R6041	T9109	T9107	npadvmod	Japan,Tokyo
R6042	T9110	T9107	punct	),Tokyo
R6043	T9111	T9105	compound	E600,upright
R6044	T9112	T9105	cc	and,upright
R6045	T9113	T9114	nmod	TE2000U,microscopes
R6046	T9114	T9105	conj	microscopes,upright
R6047	T9115	T9113	amod	inverted,TE2000U
R6048	T9116	T9114	compound	research,microscopes
R6049	T9117	T9105	acl	equipped,upright
R6050	T9118	T9117	prep	with,equipped
R6051	T9119	T9120	amod	similar,objectives
R6052	T9120	T9118	pobj	objectives,with
R6053	T9121	T9120	compound	Apochromat,objectives
R6054	T9122	T9088	punct	.,carried
R6055	T9124	T9125	nsubjpass	Images,acquired
R6056	T9126	T9124	prep	with,Images
R6057	T9127	T9128	det	the,E600
R6058	T9128	T9126	pobj	E600,with
R6059	T9129	T9128	compound	Nikon,E600
R6060	T9130	T9125	auxpass	were,acquired
R6061	T9131	T9125	prep	with,acquired
R6062	T9132	T9133	det	a,camera
R6063	T9133	T9131	pobj	camera,with
R6064	T9134	T9135	nmod	SPOT,RT
R6065	T9135	T9133	nmod	RT,camera
R6066	T9136	T9135	punct	-,RT
R6067	T9137	T9133	amod	digital,camera
R6068	T9138	T9133	acl	coupled,camera
R6069	T9139	T9138	prep	to,coupled
R6070	T9140	T9141	det	the,microscope
R6071	T9141	T9139	pobj	microscope,to
R6072	T9142	T9138	cc	and,coupled
R6073	T9143	T9138	conj	driven,coupled
R6074	T9144	T9143	agent	by,driven
R6075	T9145	T9146	compound	SPOT,v4.0
R6076	T9146	T9148	compound	v4.0,software
R6077	T9147	T9146	compound	Imaging,v4.0
R6078	T9148	T9144	pobj	software,by
R6079	T9149	T9150	punct	(,Instruments
R6080	T9150	T9148	parataxis	Instruments,software
R6081	T9151	T9150	compound	Diagnostic,Instruments
R6082	T9152	T9150	punct	", ",Instruments
R6083	T9153	T9154	compound	Sterling,Heights
R6084	T9154	T9150	npadvmod	Heights,Instruments
R6085	T9155	T9150	punct	", ",Instruments
R6086	T9156	T9150	npadvmod	Michigan,Instruments
R6087	T9157	T9150	punct	", ",Instruments
R6088	T9158	T9159	compound	United,States
R6089	T9159	T9150	npadvmod	States,Instruments
R6090	T9160	T9150	punct	),Instruments
R6091	T9161	T9125	punct	.,acquired
R6092	T9163	T9164	det	All,images
R6093	T9164	T9165	nsubjpass	images,captured
R6094	T9166	T9165	auxpass	were,captured
R6095	T9167	T9165	prep	at,captured
R6096	T9168	T9169	compound	nonsaturating,levels
R6097	T9169	T9167	pobj	levels,at
R6098	T9170	T9169	compound	integration,levels
R6099	T9171	T9169	punct	", ",levels
R6100	T9172	T9173	nummod	12,bit
R6101	T9173	T9175	nmod	bit,white
R6102	T9174	T9173	punct	-,bit
R6103	T9175	T9169	appos	white,levels
R6104	T9176	T9175	nmod	mono,white
R6105	T9177	T9175	amod	black,white
R6106	T9178	T9175	punct	/,white
R6107	T9179	T9165	punct	", ",captured
R6108	T9180	T9165	cc	and,captured
R6109	T9181	T9182	advmod	then,pseudo-colored
R6110	T9182	T9165	conj	pseudo-colored,captured
R6111	T9183	T9165	punct	.,captured
R6112	T9185	T9186	compound	Protein,colocalization
R6113	T9186	T9187	compound	colocalization,analysis
R6114	T9187	T9188	nsubjpass	analysis,carried
R6115	T9189	T9188	auxpass	was,carried
R6116	T9190	T9188	prt	out,carried
R6117	T9191	T9188	prep	on,carried
R6118	T9192	T9193	det	the,microscope
R6119	T9193	T9191	pobj	microscope,on
R6120	T9194	T9195	compound	Nikon,TE2000U
R6121	T9195	T9193	compound	TE2000U,microscope
R6122	T9196	T9193	acl	equipped,microscope
R6123	T9197	T9196	prep	with,equipped
R6124	T9198	T9199	amod	appropriate,wheels
R6125	T9199	T9197	pobj	wheels,with
R6126	T9200	T9199	nmod	excitation,wheels
R6127	T9201	T9200	cc	and,excitation
R6128	T9202	T9200	conj	emission,excitation
R6129	T9203	T9199	compound	filter,wheels
R6130	T9204	T9199	punct	", ",wheels
R6131	T9205	T9206	compound	cube,filters
R6132	T9206	T9199	appos	filters,wheels
R6133	T9207	T9199	punct	", ",wheels
R6134	T9208	T9209	nummod	100,W
R6135	T9209	T9210	compound	W,source
R6136	T9210	T9199	appos	source,wheels
R6137	T9211	T9210	compound	mercury,source
R6138	T9212	T9210	compound	light,source
R6139	T9213	T9199	punct	", ",wheels
R6140	T9214	T9215	nmod	Nomarski,DIC
R6141	T9215	T9217	nmod	DIC,optics
R6142	T9216	T9215	punct	/,DIC
R6143	T9217	T9199	appos	optics,wheels
R6144	T9218	T9215	punct	", ",DIC
R6145	T9219	T9215	cc	and,DIC
R6146	T9220	T9221	compound	Plan,Apochromat
R6147	T9221	T9215	conj	Apochromat,DIC
R6148	T9222	T9217	punct	(,optics
R6149	T9223	T9224	nummod	100,objectives
R6150	T9224	T9217	appos	objectives,optics
R6151	T9225	T9223	punct	×,100
R6152	T9226	T9223	punct	", ",100
R6153	T9227	T9223	conj	60,100
R6154	T9228	T9227	punct	×,60
R6155	T9229	T9227	punct	", ",60
R6156	T9230	T9227	cc	and,60
R6157	T9231	T9227	conj	40,60
R6158	T9232	T9231	punct	×,40
R6159	T9233	T9224	compound	oil,objectives
R6160	T9234	T9224	prep	with,objectives
R6161	T9235	T9234	pobj	NA,with
R6162	T9236	T9235	prep	of,NA
R6163	T9237	T9236	pobj	1.4,of
R6164	T9238	T9224	punct	", ",objectives
R6165	T9239	T9240	nummod	40,LWD
R6166	T9240	T9242	nmod	LWD,objectives
R6167	T9241	T9239	punct	×,40
R6168	T9242	T9224	conj	objectives,objectives
R6169	T9243	T9240	punct	", ",LWD
R6170	T9244	T9240	cc	and,LWD
R6171	T9245	T9246	nummod	20,LWD
R6172	T9246	T9240	conj	LWD,LWD
R6173	T9247	T9245	punct	×,20
R6174	T9248	T9242	cc	and,objectives
R6175	T9249	T9250	amod	encoded,Stage
R6176	T9250	T9242	conj	Stage,objectives
R6177	T9251	T9250	amod	motorized,Stage
R6178	T9252	T9250	compound	Z,Stage
R6179	T9253	T9250	punct	-,Stage
R6180	T9254	T9255	punct	(,Scientific
R6181	T9255	T9250	parataxis	Scientific,Stage
R6182	T9256	T9255	compound	Prior,Scientific
R6183	T9257	T9255	punct	", ",Scientific
R6184	T9258	T9255	npadvmod	Rockland,Scientific
R6185	T9259	T9255	punct	", ",Scientific
R6186	T9260	T9255	npadvmod	Maryland,Scientific
R6187	T9261	T9255	punct	", ",Scientific
R6188	T9262	T9263	compound	United,States
R6189	T9263	T9255	npadvmod	States,Scientific
R6190	T9264	T9255	punct	),Scientific
R6191	T9265	T9188	punct	.,carried
R6192	T9267	T9268	nsubjpass	Images,obtained
R6193	T9269	T9267	prep	with,Images
R6194	T9270	T9271	det	the,microscope
R6195	T9271	T9269	pobj	microscope,with
R6196	T9272	T9271	amod	inverted,microscope
R6197	T9273	T9274	compound	Nikon,TE2000U
R6198	T9274	T9271	compound	TE2000U,microscope
R6199	T9275	T9268	auxpass	were,obtained
R6200	T9276	T9268	advcl	using,obtained
R6201	T9277	T9278	det	a,camera
R6202	T9278	T9276	dobj	camera,using
R6203	T9279	T9278	compound	CCD,camera
R6204	T9280	T9278	punct	(,camera
R6205	T9281	T9282	compound	CoolSNAP,HQ
R6206	T9282	T9278	appos	HQ,camera
R6207	T9283	T9284	punct	;,Scientific
R6208	T9284	T9278	parataxis	Scientific,camera
R6209	T9285	T9284	compound	Roper,Scientific
R6210	T9286	T9284	punct	", ",Scientific
R6211	T9287	T9284	npadvmod	Trenton,Scientific
R6212	T9288	T9284	punct	", ",Scientific
R6213	T9289	T9290	compound	New,Jersey
R6214	T9290	T9284	npadvmod	Jersey,Scientific
R6215	T9291	T9284	punct	", ",Scientific
R6216	T9292	T9293	compound	United,States
R6217	T9293	T9284	npadvmod	States,Scientific
R6218	T9294	T9284	punct	),Scientific
R6219	T9295	T9268	punct	.,obtained
R6220	T9297	T9298	nsubjpass	Images,analyzed
R6221	T9299	T9297	acl	acquired,Images
R6222	T9300	T9299	prep	under,acquired
R6223	T9301	T9302	amod	identical,parameters
R6224	T9302	T9300	pobj	parameters,under
R6225	T9303	T9302	compound	acquisition,parameters
R6226	T9304	T9298	auxpass	were,analyzed
R6227	T9305	T9298	advcl	using,analyzed
R6228	T9306	T9307	compound	Metamorph,v6.2
R6229	T9307	T9305	dobj	v6.2,using
R6230	T9308	T9307	compound	Software,v6.2
R6231	T9309	T9310	punct	(,Imaging
R6232	T9310	T9307	parataxis	Imaging,v6.2
R6233	T9311	T9310	compound	Universal,Imaging
R6234	T9312	T9310	punct	", ",Imaging
R6235	T9313	T9310	npadvmod	Glendale,Imaging
R6236	T9314	T9310	punct	", ",Imaging
R6237	T9315	T9310	npadvmod	Wisconsin,Imaging
R6238	T9316	T9310	punct	", ",Imaging
R6239	T9317	T9318	compound	United,States
R6240	T9318	T9310	npadvmod	States,Imaging
R6241	T9319	T9310	punct	),Imaging
R6242	T9320	T9298	punct	.,analyzed
R6243	T9322	T9323	advmod	Whenever,applicable
R6244	T9323	T9324	advcl	applicable,captured
R6245	T9325	T9324	punct	", ",captured
R6246	T9326	T9327	amod	serial,stacks
R6247	T9327	T9324	nsubjpass	stacks,captured
R6248	T9328	T9327	amod	optical,stacks
R6249	T9329	T9327	compound	Z,stacks
R6250	T9330	T9327	punct	-,stacks
R6251	T9331	T9327	punct	(,stacks
R6252	T9332	T9333	quantmod	20,30
R6253	T9333	T9335	nummod	30,planes
R6254	T9334	T9333	punct	–,30
R6255	T9335	T9327	appos	planes,stacks
R6256	T9336	T9335	amod	focal,planes
R6257	T9337	T9335	prep	at,planes
R6258	T9338	T9339	nummod	100,nm
R6259	T9339	T9341	compound	nm,intervals
R6260	T9340	T9339	punct	-,nm
R6261	T9341	T9337	pobj	intervals,at
R6262	T9342	T9324	punct	),captured
R6263	T9343	T9324	auxpass	were,captured
R6264	T9344	T9324	cc	and,captured
R6265	T9345	T9346	advmod	computationally,processed
R6266	T9346	T9324	conj	processed,captured
R6267	T9347	T9346	prep	by,processed
R6268	T9348	T9349	nummod	3,D
R6269	T9349	T9351	nmod	D,methods
R6270	T9350	T9349	punct	-,D
R6271	T9351	T9347	pobj	methods,by
R6272	T9352	T9351	amod	blind,methods
R6273	T9353	T9351	compound	deconvolution,methods
R6274	T9354	T9346	prep	with,processed
R6275	T9355	T9356	det	the,software
R6276	T9356	T9354	pobj	software,with
R6277	T9357	T9356	amod	same,software
R6278	T9358	T9324	punct	.,captured
R6279	T9492	T9491	prep	of,Generation
R6280	T9493	T9494	det	a,line
R6281	T9494	T9492	pobj	line,of
R6282	T9495	T9494	compound	mouse,line
R6283	T9496	T9494	prep	with,line
R6284	T9497	T9498	det	a,locus
R6285	T9498	T9496	pobj	locus,with
R6286	T9499	T9498	amod	disrupted,locus
R6287	T9500	T9498	compound	RanBP2,locus
R6288	T9501	T9491	cc	and,Generation
R6289	T9502	T9503	amod	histological,analysis
R6290	T9503	T9491	conj	analysis,Generation
R6291	T9504	T9503	prep	of,analysis
R6292	T9505	T9506	amod	trapped,mice
R6293	T9506	T9504	pobj	mice,of
R6294	T9507	T9506	compound	RanBP2,mice
R6295	T9508	T9503	punct	.,analysis
R6296	T9510	T9511	det	A,line
R6297	T9511	T9518	nsubjpass	line,obtained
R6298	T9512	T9513	npadvmod	feeder,independent
R6299	T9513	T9511	amod	independent,line
R6300	T9514	T9513	punct	-,independent
R6301	T9515	T9511	compound	mouse,line
R6302	T9516	T9517	compound	ES,cell
R6303	T9517	T9511	compound	cell,line
R6304	T9519	T9511	acl	derived,line
R6305	T9520	T9519	prep	from,derived
R6306	T9521	T9522	det	the,strain
R6307	T9522	T9520	pobj	strain,from
R6308	T9523	T9524	compound	129P2,OlaHsd
R6309	T9524	T9522	compound	OlaHsd,strain
R6310	T9525	T9524	punct	/,OlaHsd
R6311	T9526	T9519	cc	and,derived
R6312	T9527	T9519	conj	harboring,derived
R6313	T9528	T9529	det	a,locus
R6314	T9529	T9527	dobj	locus,harboring
R6315	T9530	T9529	amod	disrupted,locus
R6316	T9531	T9529	compound	RanBP2,locus
R6317	T9532	T9529	prep	by,locus
R6318	T9533	T9534	compound	insertion,mutagenesis
R6319	T9534	T9532	pobj	mutagenesis,by
R6320	T9535	T9534	prep	with,mutagenesis
R6321	T9536	T9537	det	the,vector
R6322	T9537	T9535	pobj	vector,with
R6323	T9538	T9537	compound	gene,vector
R6324	T9539	T9537	compound	trap,vector
R6325	T9540	T9537	punct	", ",vector
R6326	T9541	T9537	appos	pGT0pfs,vector
R6327	T9542	T9543	punct	[,68
R6328	T9543	T9541	parataxis	68,pGT0pfs
R6329	T9544	T9543	nummod	67,68
R6330	T9545	T9543	punct	",",68
R6331	T9546	T9543	punct	],68
R6332	T9547	T9537	punct	(,vector
R6333	T9548	T9537	acl	generated,vector
R6334	T9549	T9548	agent	by,generated
R6335	T9550	T9551	compound	W.,Skarnes
R6336	T9551	T9549	pobj	Skarnes,by
R6337	T9552	T9518	punct	),obtained
R6338	T9553	T9518	punct	", ",obtained
R6339	T9554	T9518	auxpass	was,obtained
R6340	T9555	T9518	prep	from,obtained
R6341	T9556	T9557	det	the,Center
R6342	T9557	T9555	pobj	Center,from
R6343	T9558	T9559	compound	Mutant,Mouse
R6344	T9559	T9557	compound	Mouse,Center
R6345	T9560	T9557	compound	Regional,Center
R6346	T9561	T9557	compound	Resource,Center
R6347	T9562	T9563	punct	(,University
R6348	T9563	T9557	parataxis	University,Center
R6349	T9564	T9563	prep	of,University
R6350	T9565	T9566	compound	California,Davis
R6351	T9566	T9564	pobj	Davis,of
R6352	T9567	T9563	punct	", ",University
R6353	T9568	T9563	npadvmod	Davis,University
R6354	T9569	T9563	punct	", ",University
R6355	T9570	T9563	npadvmod	California,University
R6356	T9571	T9563	punct	", ",University
R6357	T9572	T9573	compound	United,States
R6358	T9573	T9563	npadvmod	States,University
R6359	T9574	T9563	punct	),University
R6360	T9575	T9518	punct	.,obtained
R6361	T9577	T9578	det	The,vector
R6362	T9578	T9581	nsubj	vector,contains
R6363	T9579	T9580	compound	gene,trap
R6364	T9580	T9578	compound	trap,vector
R6365	T9582	T9583	det	a,gene
R6366	T9583	T9581	dobj	gene,contains
R6367	T9584	T9583	amod	promoterless,gene
R6368	T9585	T9583	compound	neo-lacZ,gene
R6369	T9586	T9583	compound	fusion,gene
R6370	T9587	T9583	prep	with,gene
R6371	T9588	T9589	det	a,site
R6372	T9589	T9587	pobj	site,with
R6373	T9590	T9589	compound	splice,site
R6374	T9591	T9589	compound	acceptor,site
R6375	T9592	T9589	prep	at,site
R6376	T9593	T9594	det	the,end
R6377	T9594	T9592	pobj	end,at
R6378	T9595	T9594	nummod	5,end
R6379	T9596	T9595	punct	′,5
R6380	T9597	T9589	acl	followed,site
R6381	T9598	T9597	agent	by,followed
R6382	T9599	T9600	det	an,site
R6383	T9600	T9598	pobj	site,by
R6384	T9601	T9600	amod	internal,site
R6385	T9602	T9600	compound	ribosome,site
R6386	T9603	T9600	compound	entry,site
R6387	T9604	T9600	punct	(,site
R6388	T9605	T9600	appos	IRES,site
R6389	T9606	T9600	punct	),site
R6390	T9607	T9600	cc	and,site
R6391	T9608	T9609	det	the,cassette
R6392	T9609	T9600	conj	cassette,site
R6393	T9610	T9609	amod	human,cassette
R6394	T9611	T9612	amod	placental,phosphatase
R6395	T9612	T9609	nmod	phosphatase,cassette
R6396	T9613	T9612	nmod	alkaline,phosphatase
R6397	T9614	T9612	punct	(,phosphatase
R6398	T9615	T9612	appos	PLAP,phosphatase
R6399	T9616	T9609	punct	),cassette
R6400	T9617	T9609	compound	reporter,cassette
R6401	T9618	T9581	punct	.,contains
R6402	T9620	T9621	det	The,line
R6403	T9621	T9625	nsubjpass	line,injected
R6404	T9622	T9623	compound	RanBP2,129Ola
R6405	T9623	T9621	compound	129Ola,line
R6406	T9624	T9621	compound	ES,line
R6407	T9626	T9625	auxpass	was,injected
R6408	T9627	T9625	prep	into,injected
R6409	T9628	T9629	compound	C57BL,6J
R6410	T9629	T9631	compound	6J,blastocysts
R6411	T9630	T9629	punct	/,6J
R6412	T9631	T9627	pobj	blastocysts,into
R6413	T9632	T9625	cc	and,injected
R6414	T9633	T9634	nummod	four,chimeras
R6415	T9634	T9636	nsubjpass	chimeras,generated
R6416	T9635	T9634	amod	male,chimeras
R6417	T9636	T9625	conj	generated,injected
R6418	T9637	T9636	auxpass	were,generated
R6419	T9638	T9636	punct	.,generated
R6420	T9640	T9641	nsubjpass	These,bred
R6421	T9642	T9641	auxpass	were,bred
R6422	T9643	T9641	prep	into,bred
R6423	T9644	T9645	nmod	129P2,OlaHsd
R6424	T9645	T9647	nmod	OlaHsd,females
R6425	T9646	T9645	punct	/,OlaHsd
R6426	T9647	T9643	pobj	females,into
R6427	T9648	T9649	punct	(,Harlan
R6428	T9649	T9645	parataxis	Harlan,OlaHsd
R6429	T9650	T9649	punct	", ",Harlan
R6430	T9651	T9649	npadvmod	Indianapolis,Harlan
R6431	T9652	T9649	punct	", ",Harlan
R6432	T9653	T9649	npadvmod	Indiana,Harlan
R6433	T9654	T9649	punct	", ",Harlan
R6434	T9655	T9656	compound	United,States
R6435	T9656	T9649	npadvmod	States,Harlan
R6436	T9657	T9649	punct	),Harlan
R6437	T9658	T9645	cc	and,OlaHsd
R6438	T9659	T9660	compound	C57BL,6J
R6439	T9660	T9645	conj	6J,OlaHsd
R6440	T9661	T9660	punct	/,6J
R6441	T9662	T9663	punct	(,Laboratory
R6442	T9663	T9660	parataxis	Laboratory,6J
R6443	T9664	T9663	compound	Jackson,Laboratory
R6444	T9665	T9663	punct	", ",Laboratory
R6445	T9666	T9667	compound	Bar,Harbor
R6446	T9667	T9663	npadvmod	Harbor,Laboratory
R6447	T9668	T9663	punct	", ",Laboratory
R6448	T9669	T9663	npadvmod	Maine,Laboratory
R6449	T9670	T9663	punct	", ",Laboratory
R6450	T9671	T9672	compound	United,States
R6451	T9672	T9663	npadvmod	States,Laboratory
R6452	T9673	T9663	punct	),Laboratory
R6453	T9674	T9641	punct	.,bred
R6454	T9676	T9677	det	The,lines
R6455	T9677	T9683	nsubjpass	lines,tested
R6456	T9678	T9677	nummod	two,lines
R6457	T9679	T9677	amod	resulting,lines
R6458	T9680	T9679	cc	and,resulting
R6459	T9681	T9679	conj	independent,resulting
R6460	T9682	T9677	compound	F1s,lines
R6461	T9684	T9677	punct	", ",lines
R6462	T9685	T9677	prep	with,lines
R6463	T9686	T9687	compound	129P2,OlaHsd
R6464	T9687	T9685	pobj	OlaHsd,with
R6465	T9688	T9687	punct	/,OlaHsd
R6466	T9689	T9687	amod	inbred,OlaHsd
R6467	T9690	T9687	cc	and,OlaHsd
R6468	T9691	T9692	nmod	129P2,6J
R6469	T9692	T9698	nmod	6J,backgrounds
R6470	T9693	T9692	punct	/,6J
R6471	T9694	T9692	nmod	OlaHsd,6J
R6472	T9695	T9692	punct	/,6J
R6473	T9696	T9692	nmod	C57BL,6J
R6474	T9697	T9692	punct	/,6J
R6475	T9698	T9687	conj	backgrounds,OlaHsd
R6476	T9699	T9692	amod	mixed,6J
R6477	T9700	T9692	amod	genetic,6J
R6478	T9701	T9683	punct	", ",tested
R6479	T9702	T9683	auxpass	were,tested
R6480	T9703	T9683	prep	for,tested
R6481	T9704	T9705	compound	germline,transmission
R6482	T9705	T9703	pobj	transmission,for
R6483	T9706	T9683	prep	by,tested
R6484	T9707	T9708	nmod	PCR,analysis
R6485	T9708	T9706	pobj	analysis,by
R6486	T9709	T9707	cc	and,PCR
R6487	T9710	T9711	compound	Southern,blot
R6488	T9711	T9707	conj	blot,PCR
R6489	T9712	T9708	prep	of,analysis
R6490	T9713	T9714	nmod	tail,DNA
R6491	T9714	T9712	pobj	DNA,of
R6492	T9715	T9714	amod	genomic,DNA
R6493	T9716	T9683	punct	.,tested
R6494	T9718	T9719	nmod	β,gal
R6495	T9719	T9721	nmod	gal,activities
R6496	T9720	T9719	punct	-,gal
R6497	T9721	T9724	nsubjpass	activities,detected
R6498	T9722	T9719	cc	and,gal
R6499	T9723	T9719	conj	PLAP,gal
R6500	T9725	T9721	prep	in,activities
R6501	T9726	T9727	amod	whole,mount
R6502	T9727	T9728	compound	mount,embryos
R6503	T9728	T9725	pobj	embryos,in
R6504	T9729	T9728	cc	and,embryos
R6505	T9730	T9731	amod	retinal,sections
R6506	T9731	T9728	conj	sections,embryos
R6507	T9732	T9724	auxpass	were,detected
R6508	T9733	T9724	punct	", ",detected
R6509	T9734	T9724	advmod	respectively,detected
R6510	T9735	T9724	punct	", ",detected
R6511	T9736	T9724	prep	by,detected
R6512	T9737	T9736	pobj	incubation,by
R6513	T9738	T9737	prep	with,incubation
R6514	T9739	T9740	nummod	5,bromo
R6515	T9740	T9742	nmod	bromo,galactopyranoside
R6516	T9741	T9740	punct	-,bromo
R6517	T9742	T9755	nmod	galactopyranoside,buffer
R6518	T9743	T9742	punct	-,galactopyranoside
R6519	T9744	T9742	nummod	4,galactopyranoside
R6520	T9745	T9744	punct	-,4
R6521	T9746	T9742	nmod	chloro,galactopyranoside
R6522	T9747	T9742	punct	-,galactopyranoside
R6523	T9748	T9749	nummod	3,β
R6524	T9749	T9742	nmod	β,galactopyranoside
R6525	T9750	T9749	punct	-,β
R6526	T9751	T9749	nmod	indolyl,β
R6527	T9752	T9742	punct	-,galactopyranoside
R6528	T9753	T9742	nmod	D,galactopyranoside
R6529	T9754	T9742	punct	-,galactopyranoside
R6530	T9755	T9738	pobj	buffer,with
R6531	T9756	T9742	cc	and,galactopyranoside
R6532	T9757	T9742	conj	AP,galactopyranoside
R6533	T9758	T9755	compound	staining,buffer
R6534	T9759	T9760	punct	(,tetrazolium
R6535	T9760	T9737	parataxis	tetrazolium,incubation
R6536	T9761	T9762	nummod	0.1,mg
R6537	T9762	T9763	nmod	mg,phosphate
R6538	T9763	T9760	dep	phosphate,tetrazolium
R6539	T9764	T9765	punct	/,ml
R6540	T9765	T9762	prep	ml,mg
R6541	T9766	T9767	nummod	5,bromo
R6542	T9767	T9763	nmod	bromo,phosphate
R6543	T9768	T9767	punct	-,bromo
R6544	T9769	T9763	punct	-,phosphate
R6545	T9770	T9771	nummod	4,chloro
R6546	T9771	T9763	nmod	chloro,phosphate
R6547	T9772	T9771	punct	-,chloro
R6548	T9773	T9763	punct	-,phosphate
R6549	T9774	T9763	nummod	3,phosphate
R6550	T9775	T9763	punct	-,phosphate
R6551	T9776	T9763	compound	indolyl,phosphate
R6552	T9777	T9760	punct	", ",tetrazolium
R6553	T9778	T9779	nummod	1,mg
R6554	T9779	T9760	nmod	mg,tetrazolium
R6555	T9780	T9781	punct	/,ml
R6556	T9781	T9779	prep	ml,mg
R6557	T9782	T9760	amod	nitroblue,tetrazolium
R6558	T9783	T9760	prep	in,tetrazolium
R6559	T9784	T9785	nummod	100,mM
R6560	T9785	T9786	compound	mM,HCl
R6561	T9786	T9783	pobj	HCl,in
R6562	T9787	T9786	compound	Tris,HCl
R6563	T9788	T9786	punct	-,HCl
R6564	T9789	T9790	punct	(,pH
R6565	T9790	T9786	parataxis	pH,HCl
R6566	T9791	T9790	nummod	9.5,pH
R6567	T9792	T9790	punct	),pH
R6568	T9793	T9786	punct	", ",HCl
R6569	T9794	T9795	nummod	100,mM
R6570	T9795	T9796	compound	mM,NaCl
R6571	T9796	T9786	appos	NaCl,HCl
R6572	T9797	T9786	punct	", ",HCl
R6573	T9798	T9799	nummod	5,mM
R6574	T9799	T9800	compound	mM,MgCl2
R6575	T9800	T9786	appos	MgCl2,HCl
R6576	T9801	T9760	punct	),tetrazolium
R6577	T9802	T9803	mark	as,described
R6578	T9803	T9724	advcl	described,detected
R6579	T9804	T9803	advmod	elsewhere,described
R6580	T9805	T9806	punct	[,68
R6581	T9806	T9803	parataxis	68,described
R6582	T9807	T9806	nummod	67,68
R6583	T9808	T9806	punct	",",68
R6584	T9809	T9806	punct	],68
R6585	T9810	T9724	punct	.,detected
R6586	T9862	T9863	compound	Western,blot
R6587	T9864	T9863	cc	and,blot
R6588	T9865	T9866	compound	quantitation,analysis
R6589	T9866	T9863	conj	analysis,blot
R6590	T9867	T9863	prep	of,blot
R6591	T9868	T9869	compound	protein,expression
R6592	T9869	T9867	pobj	expression,of
R6593	T9870	T9869	cc	and,expression
R6594	T9871	T9872	compound	ATP,levels
R6595	T9872	T9869	conj	levels,expression
R6596	T9873	T9863	punct	.,blot
R6597	T9875	T9876	nsubjpass	Homogenates,prepared
R6598	T9877	T9875	cc	and,Homogenates
R6599	T9878	T9879	npadvmod	detergent,solubilized
R6600	T9879	T9880	amod	solubilized,extracts
R6601	T9880	T9875	conj	extracts,Homogenates
R6602	T9881	T9875	prep	of,Homogenates
R6603	T9882	T9881	pobj	tissues,of
R6604	T9883	T9882	cc	and,tissues
R6605	T9884	T9882	conj	immunoblots,tissues
R6606	T9885	T9876	auxpass	were,prepared
R6607	T9886	T9887	mark	as,described
R6608	T9887	T9876	advcl	described,prepared
R6609	T9888	T9887	advmod	elsewhere,described
R6610	T9889	T9890	punct	[,69
R6611	T9890	T9887	parataxis	69,described
R6612	T9891	T9890	punct	],69
R6613	T9892	T9876	punct	.,prepared
R6614	T9894	T9895	nsubjpass	Quantitation,carried
R6615	T9896	T9894	prep	of,Quantitation
R6616	T9897	T9898	compound	immunoblot,bands
R6617	T9898	T9896	pobj	bands,of
R6618	T9899	T9894	acl	calibrated,Quantitation
R6619	T9900	T9899	prep	against,calibrated
R6620	T9901	T9902	amod	internal,bands
R6621	T9902	T9900	pobj	bands,against
R6622	T9903	T9902	compound	marker,bands
R6623	T9904	T9902	punct	", ",bands
R6624	T9905	T9906	amod	such,as
R6625	T9906	T9902	prep	as,bands
R6626	T9907	T9906	pobj	mHsp70,as
R6627	T9908	T9907	cc	and,mHsp70
R6628	T9909	T9907	conj	Nup153,mHsp70
R6629	T9910	T9895	punct	", ",carried
R6630	T9911	T9895	auxpass	was,carried
R6631	T9912	T9895	prt	out,carried
R6632	T9913	T9895	prep	with,carried
R6633	T9914	T9915	compound	Gel,Analyzer
R6634	T9915	T9913	pobj	Analyzer,with
R6635	T9916	T9915	compound	Pro,Analyzer
R6636	T9917	T9918	punct	(,MediaCybernetics
R6637	T9918	T9915	parataxis	MediaCybernetics,Analyzer
R6638	T9919	T9918	punct	", ",MediaCybernetics
R6639	T9920	T9921	compound	San,Diego
R6640	T9921	T9918	npadvmod	Diego,MediaCybernetics
R6641	T9922	T9918	punct	", ",MediaCybernetics
R6642	T9923	T9918	npadvmod	California,MediaCybernetics
R6643	T9924	T9918	punct	", ",MediaCybernetics
R6644	T9925	T9926	compound	United,States
R6645	T9926	T9918	npadvmod	States,MediaCybernetics
R6646	T9927	T9918	punct	),MediaCybernetics
R6647	T9928	T9895	punct	.,carried
R6648	T9930	T9931	prep	For,homogenized
R6649	T9932	T9930	pobj	determination,For
R6650	T9933	T9932	prep	of,determination
R6651	T9934	T9935	compound	ATP,levels
R6652	T9935	T9933	pobj	levels,of
R6653	T9936	T9931	punct	", ",homogenized
R6654	T9937	T9938	advmod	freshly,dissected
R6655	T9938	T9939	amod	dissected,tissues
R6656	T9939	T9931	nsubjpass	tissues,homogenized
R6657	T9940	T9938	cc	and,dissected
R6658	T9941	T9942	npadvmod	flash,frozen
R6659	T9942	T9938	conj	frozen,dissected
R6660	T9943	T9942	punct	-,frozen
R6661	T9944	T9931	auxpass	were,homogenized
R6662	T9945	T9931	prep	in,homogenized
R6663	T9946	T9947	nummod	2.5,%
R6664	T9947	T9945	pobj	%,in
R6665	T9948	T9949	amod	trichloroacetic,acid
R6666	T9949	T9947	appos	acid,%
R6667	T9950	T9949	punct	(,acid
R6668	T9951	T9949	appos	TCA,acid
R6669	T9952	T9931	punct	),homogenized
R6670	T9953	T9931	punct	", ",homogenized
R6671	T9954	T9931	conj	neutralized,homogenized
R6672	T9955	T9954	punct	", ",neutralized
R6673	T9956	T9954	cc	and,neutralized
R6674	T9957	T9954	conj	diluted,neutralized
R6675	T9958	T9957	prep	to,diluted
R6676	T9959	T9960	det	a,concentration
R6677	T9960	T9958	pobj	concentration,to
R6678	T9961	T9960	amod	final,concentration
R6679	T9962	T9960	prep	of,concentration
R6680	T9963	T9964	nummod	0.1,%
R6681	T9964	T9962	pobj	%,of
R6682	T9965	T9957	prep	with,diluted
R6683	T9966	T9967	compound	Tris,Acetate
R6684	T9967	T9965	pobj	Acetate,with
R6685	T9968	T9967	punct	-,Acetate
R6686	T9969	T9970	punct	(,pH
R6687	T9970	T9967	parataxis	pH,Acetate
R6688	T9971	T9970	nummod	7.75,pH
R6689	T9972	T9970	punct	),pH
R6690	T9973	T9931	punct	.,homogenized
R6691	T9975	T9976	compound	ATP,measurements
R6692	T9976	T9977	nsubjpass	measurements,carried
R6693	T9978	T9977	auxpass	were,carried
R6694	T9979	T9977	prt	out,carried
R6695	T9980	T9977	prep	with,carried
R6696	T9981	T9982	det	the,Kit
R6697	T9982	T9980	pobj	Kit,with
R6698	T9983	T9982	compound	ENLITEN,Kit
R6699	T9984	T9985	compound	ATP,System
R6700	T9985	T9982	compound	System,Kit
R6701	T9986	T9985	compound	Assay,System
R6702	T9987	T9988	compound	Bioluminescence,Detection
R6703	T9988	T9982	compound	Detection,Kit
R6704	T9989	T9982	prep	for,Kit
R6705	T9990	T9989	pobj	ATP,for
R6706	T9991	T9992	punct	(,Promega
R6707	T9992	T9982	parataxis	Promega,Kit
R6708	T9993	T9992	punct	", ",Promega
R6709	T9994	T9992	npadvmod	Madison,Promega
R6710	T9995	T9992	punct	", ",Promega
R6711	T9996	T9992	npadvmod	Wisconsin,Promega
R6712	T9997	T9992	punct	", ",Promega
R6713	T9998	T9999	compound	United,States
R6714	T9999	T9992	npadvmod	States,Promega
R6715	T10000	T9992	punct	),Promega
R6716	T10001	T9977	prep	as,carried
R6717	T10002	T10001	prep	per,as
R6718	T10003	T10004	det	the,manufacturer
R6719	T10004	T10005	poss	manufacturer,instruction
R6720	T10005	T10002	pobj	instruction,per
R6721	T10006	T10004	case	's,manufacturer
R6722	T10007	T9977	prep	in,carried
R6723	T10008	T10009	compound	SpectraMax,M5
R6724	T10009	T10007	pobj	M5,in
R6725	T10010	T10009	punct	-,M5
R6726	T10011	T10012	punct	(,Devices
R6727	T10012	T10009	parataxis	Devices,M5
R6728	T10013	T10012	compound	Molecular,Devices
R6729	T10014	T10012	punct	", ",Devices
R6730	T10015	T10012	npadvmod	Sunnyvale,Devices
R6731	T10016	T10012	punct	", ",Devices
R6732	T10017	T10012	npadvmod	California,Devices
R6733	T10018	T10012	punct	", ",Devices
R6734	T10019	T10020	compound	United,States
R6735	T10020	T10012	npadvmod	States,Devices
R6736	T10021	T10012	punct	),Devices
R6737	T10022	T9977	punct	.,carried
R6738	T10024	T10025	compound	Protein,concentration
R6739	T10025	T10026	nsubjpass	concentration,measured
R6740	T10027	T10026	auxpass	was,measured
R6741	T10028	T10026	prep	by,measured
R6742	T10029	T10030	det	the,method
R6743	T10030	T10028	pobj	method,by
R6744	T10031	T10030	compound	BCA,method
R6745	T10032	T10033	punct	(,BioRad
R6746	T10033	T10030	parataxis	BioRad,method
R6747	T10034	T10033	punct	", ",BioRad
R6748	T10035	T10033	npadvmod	Hercules,BioRad
R6749	T10036	T10033	punct	", ",BioRad
R6750	T10037	T10033	npadvmod	California,BioRad
R6751	T10038	T10033	punct	", ",BioRad
R6752	T10039	T10040	compound	United,States
R6753	T10040	T10033	npadvmod	States,BioRad
R6754	T10041	T10033	punct	),BioRad
R6755	T10042	T10026	advcl	using,measured
R6756	T10043	T10042	dobj	BSA,using
R6757	T10044	T10042	prep	as,using
R6758	T10045	T10046	det	a,standard
R6759	T10046	T10044	pobj	standard,as
R6760	T10047	T10026	punct	.,measured
R6761	T10131	T10132	amod	Metabolic,assays
R6762	T10133	T10132	punct	.,assays
R6763	T10135	T10136	nummod	Two,groups
R6764	T10136	T10137	nsubjpass	groups,kept
R6765	T10138	T10136	prep	of,groups
R6766	T10139	T10140	amod	male,mice
R6767	T10140	T10138	pobj	mice,of
R6768	T10141	T10137	auxpass	were,kept
R6769	T10142	T10137	prep	under,kept
R6770	T10143	T10144	amod	separate,diet
R6771	T10144	T10142	pobj	diet,under
R6772	T10145	T10137	prep	since,kept
R6773	T10146	T10145	pobj	gestation,since
R6774	T10147	T10146	cc	or,gestation
R6775	T10148	T10146	conj	birth,gestation
R6776	T10149	T10137	punct	.,kept
R6777	T10151	T10152	nummod	One,group
R6778	T10152	T10153	nsubjpass	group,fed
R6779	T10154	T10153	auxpass	was,fed
R6780	T10155	T10153	prep	with,fed
R6781	T10156	T10157	det	a,diet
R6782	T10157	T10155	pobj	diet,with
R6783	T10158	T10157	amod	normal,diet
R6784	T10159	T10157	compound	chow,diet
R6785	T10160	T10157	punct	(,diet
R6786	T10161	T10162	compound	PicoLab,Diet
R6787	T10162	T10157	appos	Diet,diet
R6788	T10163	T10162	compound	Rodent,Diet
R6789	T10164	T10162	nummod	20,Diet
R6790	T10165	T10162	punct	−,Diet
R6791	T10166	T10162	nummod	5053,Diet
R6792	T10167	T10168	punct	;,LabDiet
R6793	T10168	T10157	parataxis	LabDiet,diet
R6794	T10169	T10168	punct	", ",LabDiet
R6795	T10170	T10168	npadvmod	Richmond,LabDiet
R6796	T10171	T10168	punct	", ",LabDiet
R6797	T10172	T10168	npadvmod	Indiana,LabDiet
R6798	T10173	T10168	punct	", ",LabDiet
R6799	T10174	T10175	compound	United,States
R6800	T10175	T10168	npadvmod	States,LabDiet
R6801	T10176	T10168	punct	),LabDiet
R6802	T10177	T10153	punct	", ",fed
R6803	T10178	T10179	mark	while,placed
R6804	T10179	T10153	advcl	placed,fed
R6805	T10180	T10181	det	the,other
R6806	T10181	T10179	nsubjpass	other,placed
R6807	T10182	T10179	auxpass	was,placed
R6808	T10183	T10179	prep	on,placed
R6809	T10184	T10185	det	a,diet
R6810	T10185	T10183	pobj	diet,on
R6811	T10186	T10187	amod	higher,energy
R6812	T10187	T10185	nmod	energy,diet
R6813	T10188	T10187	cc	and,energy
R6814	T10189	T10187	conj	fat,energy
R6815	T10190	T10185	punct	(,diet
R6816	T10191	T10192	compound	PicoLab,Diet
R6817	T10192	T10185	appos	Diet,diet
R6818	T10193	T10192	compound	Mouse,Diet
R6819	T10194	T10192	nummod	20,Diet
R6820	T10195	T10192	punct	−,Diet
R6821	T10196	T10192	nummod	5058,Diet
R6822	T10197	T10198	punct	;,LabDiet
R6823	T10198	T10185	parataxis	LabDiet,diet
R6824	T10199	T10198	punct	),LabDiet
R6825	T10200	T10153	punct	.,fed
R6826	T10202	T10203	compound	Glucose,tolerance
R6827	T10203	T10204	compound	tolerance,test
R6828	T10204	T10205	nsubjpass	test,performed
R6829	T10206	T10205	auxpass	was,performed
R6830	T10207	T10205	prep	on,performed
R6831	T10208	T10209	amod	overnight,fasted
R6832	T10209	T10211	amod	fasted,mice
R6833	T10210	T10209	punct	-,fasted
R6834	T10211	T10207	pobj	mice,on
R6835	T10212	T10211	acl	injected,mice
R6836	T10213	T10212	advmod	intraperitoneally,injected
R6837	T10214	T10212	prep	with,injected
R6838	T10215	T10216	nummod	2,g
R6839	T10216	T10214	pobj	g,with
R6840	T10217	T10216	prep	of,g
R6841	T10218	T10217	pobj	glucose,of
R6842	T10219	T10216	prep	per,g
R6843	T10220	T10219	pobj	kg,per
R6844	T10221	T10220	prep	of,kg
R6845	T10222	T10223	compound	body,weight
R6846	T10223	T10221	pobj	weight,of
R6847	T10224	T10205	punct	.,performed
R6848	T10226	T10227	amod	Venous,values
R6849	T10227	T10230	nsubjpass	values,determined
R6850	T10228	T10229	compound	blood,glucose
R6851	T10229	T10227	compound	glucose,values
R6852	T10231	T10230	auxpass	were,determined
R6853	T10232	T10230	prep	at,determined
R6854	T10233	T10234	advmod	immediately,before
R6855	T10234	T10232	prep	before,at
R6856	T10235	T10236	punct	(,min
R6857	T10236	T10234	parataxis	min,before
R6858	T10237	T10236	nummod	0,min
R6859	T10238	T10236	punct	),min
R6860	T10239	T10234	punct	", ",before
R6861	T10240	T10234	cc	and,before
R6862	T10241	T10242	nummod	15,min
R6863	T10242	T10250	npadvmod	min,injection
R6864	T10243	T10241	punct	", ",15
R6865	T10244	T10241	conj	30,15
R6866	T10245	T10244	punct	", ",30
R6867	T10246	T10244	conj	60,30
R6868	T10247	T10246	punct	", ",60
R6869	T10248	T10246	cc	and,60
R6870	T10249	T10246	conj	120,60
R6871	T10250	T10234	conj	injection,before
R6872	T10251	T10242	prep	after,min
R6873	T10252	T10250	det	the,injection
R6874	T10253	T10250	prep	with,injection
R6875	T10254	T10255	det	an,instrument
R6876	T10255	T10253	pobj	instrument,with
R6877	T10256	T10255	amod	automatic,instrument
R6878	T10257	T10255	compound	glucose,instrument
R6879	T10258	T10255	compound	monitor,instrument
R6880	T10259	T10255	punct	(,instrument
R6881	T10260	T10261	nummod	One,Ultra
R6882	T10261	T10255	appos	Ultra,instrument
R6883	T10262	T10261	compound	Touch,Ultra
R6884	T10263	T10264	punct	", ",Lifescan
R6885	T10264	T10255	parataxis	Lifescan,instrument
R6886	T10265	T10264	punct	),Lifescan
R6887	T10266	T10230	punct	.,determined
R6888	T10268	T10269	compound	Insulin,tolerance
R6889	T10269	T10270	compound	tolerance,test
R6890	T10270	T10271	nsubjpass	test,performed
R6891	T10272	T10271	auxpass	was,performed
R6892	T10273	T10274	det	the,way
R6893	T10274	T10271	npadvmod	way,performed
R6894	T10275	T10274	amod	same,way
R6895	T10276	T10274	prep	as,way
R6896	T10277	T10276	pobj	that,as
R6897	T10278	T10277	acl	described,that
R6898	T10279	T10278	prep	for,described
R6899	T10280	T10281	det	the,test
R6900	T10281	T10279	pobj	test,for
R6901	T10282	T10283	compound	glucose,tolerance
R6902	T10283	T10281	compound	tolerance,test
R6903	T10284	T10274	prep	with,way
R6904	T10285	T10286	det	the,exception
R6905	T10286	T10284	pobj	exception,with
R6906	T10287	T10288	mark	that,injected
R6907	T10288	T10286	acl	injected,exception
R6908	T10289	T10290	amod	human,insulin
R6909	T10290	T10288	nsubjpass	insulin,injected
R6910	T10291	T10292	punct	(,U
R6911	T10292	T10290	parataxis	U,insulin
R6912	T10293	T10292	nummod	0.75,U
R6913	T10294	T10295	punct	/,kg
R6914	T10295	T10292	prep	kg,U
R6915	T10296	T10295	prep	of,kg
R6916	T10297	T10298	compound	body,mass
R6917	T10298	T10296	pobj	mass,of
R6918	T10299	T10292	punct	),U
R6919	T10300	T10288	auxpass	was,injected
R6920	T10301	T10288	advmod	intraperitoneally,injected
R6921	T10302	T10288	prep	in,injected
R6922	T10303	T10304	amod	non-fasted,mice
R6923	T10304	T10302	pobj	mice,in
R6924	T10305	T10271	punct	.,performed
R6925	T10307	T10308	compound	Pyruvate,challenge
R6926	T10308	T10309	compound	challenge,test
R6927	T10309	T10310	nsubjpass	test,performed
R6928	T10311	T10310	auxpass	was,performed
R6929	T10312	T10313	det	the,way
R6930	T10313	T10310	npadvmod	way,performed
R6931	T10314	T10313	amod	same,way
R6932	T10315	T10313	prep	as,way
R6933	T10316	T10315	pobj	that,as
R6934	T10317	T10316	acl	described,that
R6935	T10318	T10317	prep	for,described
R6936	T10319	T10320	det	the,test
R6937	T10320	T10318	pobj	test,for
R6938	T10321	T10322	compound	glucose,tolerance
R6939	T10322	T10320	compound	tolerance,test
R6940	T10323	T10313	prep	with,way
R6941	T10324	T10325	det	the,exception
R6942	T10325	T10323	pobj	exception,with
R6943	T10326	T10327	mark	that,injected
R6944	T10327	T10325	acl	injected,exception
R6945	T10328	T10327	nsubjpass	pyruvate,injected
R6946	T10329	T10328	acl	dissolved,pyruvate
R6947	T10330	T10329	prep	in,dissolved
R6948	T10331	T10330	pobj	saline,in
R6949	T10332	T10333	punct	(,g
R6950	T10333	T10331	parataxis	g,saline
R6951	T10334	T10333	nummod	2,g
R6952	T10335	T10336	punct	/,kg
R6953	T10336	T10333	prep	kg,g
R6954	T10337	T10336	prep	of,kg
R6955	T10338	T10339	compound	body,mass
R6956	T10339	T10337	pobj	mass,of
R6957	T10340	T10333	punct	),g
R6958	T10341	T10327	auxpass	was,injected
R6959	T10342	T10327	advmod	intraperitoneally,injected
R6960	T10343	T10327	prep	in,injected
R6961	T10344	T10345	amod	overnight,fasted
R6962	T10345	T10347	amod	fasted,mice
R6963	T10346	T10345	punct	-,fasted
R6964	T10347	T10343	pobj	mice,in
R6965	T10348	T10310	punct	.,performed
R6966	T10408	T10407	punct	.,Electroretinography
R6967	T10410	T10411	nummod	Five,mice
R6968	T10411	T10428	nsubjpass	mice,kept
R6969	T10412	T10413	punct	~,6
R6970	T10413	T10414	nummod	6,mo
R6971	T10414	T10416	npadvmod	mo,old
R6972	T10415	T10414	punct	-,mo
R6973	T10416	T10411	amod	old,mice
R6974	T10417	T10416	punct	-,old
R6975	T10418	T10411	amod	male,mice
R6976	T10419	T10411	nmod	RanBP2,mice
R6977	T10420	T10419	punct	+,RanBP2
R6978	T10421	T10419	punct	/,RanBP2
R6979	T10422	T10419	punct	+,RanBP2
R6980	T10423	T10419	cc	and,RanBP2
R6981	T10424	T10419	conj	RanBP2,RanBP2
R6982	T10425	T10424	punct	+,RanBP2
R6983	T10426	T10424	punct	/,RanBP2
R6984	T10427	T10424	punct	−,RanBP2
R6985	T10429	T10411	prep	in,mice
R6986	T10430	T10431	compound	129ola,background
R6987	T10431	T10429	pobj	background,in
R6988	T10432	T10428	auxpass	were,kept
R6989	T10433	T10428	prep	in,kept
R6990	T10434	T10435	nmod	dim,light
R6991	T10435	T10433	pobj	light,in
R6992	T10436	T10435	amod	cyclic,light
R6993	T10437	T10438	punct	(,lux
R6994	T10438	T10435	parataxis	lux,light
R6995	T10439	T10438	nummod	10,lux
R6996	T10440	T10438	punct	),lux
R6997	T10441	T10428	prep	for,kept
R6998	T10442	T10443	nummod	2,wk
R6999	T10443	T10441	pobj	wk,for
R7000	T10444	T10445	mark	before,recorded
R7001	T10445	T10428	advcl	recorded,kept
R7002	T10446	T10447	amod	electroretinographic,responses
R7003	T10447	T10445	nsubjpass	responses,recorded
R7004	T10448	T10445	auxpass	were,recorded
R7005	T10449	T10428	punct	.,kept
R7006	T10451	T10452	det	The,mice
R7007	T10452	T10453	nsubjpass	mice,anesthetized
R7008	T10454	T10453	auxpass	were,anesthetized
R7009	T10455	T10453	advmod	intraperitoneally,anesthetized
R7010	T10456	T10453	prep	with,anesthetized
R7011	T10457	T10456	pobj	ketamine,with
R7012	T10458	T10459	punct	(,mg
R7013	T10459	T10457	parataxis	mg,ketamine
R7014	T10460	T10459	nummod	80,mg
R7015	T10461	T10462	punct	/,kg
R7016	T10462	T10459	prep	kg,mg
R7017	T10463	T10459	punct	),mg
R7018	T10464	T10457	cc	and,ketamine
R7019	T10465	T10457	conj	xylazine,ketamine
R7020	T10466	T10467	punct	(,mg
R7021	T10467	T10465	parataxis	mg,xylazine
R7022	T10468	T10467	nummod	4,mg
R7023	T10469	T10470	punct	/,kg
R7024	T10470	T10467	prep	kg,mg
R7025	T10471	T10467	punct	),mg
R7026	T10472	T10453	punct	.,anesthetized
R7027	T10474	T10475	compound	Body,temperature
R7028	T10475	T10476	nsubjpass	temperature,maintained
R7029	T10477	T10476	auxpass	was,maintained
R7030	T10478	T10476	prep	with,maintained
R7031	T10479	T10480	det	a,pad
R7032	T10480	T10478	pobj	pad,with
R7033	T10481	T10480	compound	heating,pad
R7034	T10482	T10476	prep	at,maintained
R7035	T10483	T10484	quantmod	37.5,1
R7036	T10484	T10486	nummod	1,°C
R7037	T10485	T10484	punct	±,1
R7038	T10486	T10482	pobj	°C,at
R7039	T10487	T10476	prep	throughout,maintained
R7040	T10488	T10489	det	the,recording
R7041	T10489	T10487	pobj	recording,throughout
R7042	T10490	T10476	punct	.,maintained
R7043	T10492	T10493	det	The,pupils
R7044	T10493	T10494	nsubjpass	pupils,dilated
R7045	T10495	T10494	auxpass	were,dilated
R7046	T10496	T10494	advmod	fully,dilated
R7047	T10497	T10494	prep	with,dilated
R7048	T10498	T10499	nummod	0.5,%
R7049	T10499	T10500	compound	%,tropicamide
R7050	T10500	T10497	pobj	tropicamide,with
R7051	T10501	T10500	cc	and,tropicamide
R7052	T10502	T10503	nummod	2.5,%
R7053	T10503	T10504	compound	%,HCl
R7054	T10504	T10500	conj	HCl,tropicamide
R7055	T10505	T10504	compound	phenylephrine,HCl
R7056	T10506	T10494	punct	", ",dilated
R7057	T10507	T10494	cc	and,dilated
R7058	T10508	T10509	det	both,eyes
R7059	T10509	T10510	nsubjpass	eyes,recorded
R7060	T10510	T10494	conj	recorded,dilated
R7061	T10511	T10510	auxpass	were,recorded
R7062	T10512	T10510	advmod	simultaneously,recorded
R7063	T10513	T10510	punct	.,recorded
R7064	T10515	T10516	amod	Corneal,loops
R7065	T10516	T10520	nsubjpass	loops,used
R7066	T10517	T10518	amod	gold,wire
R7067	T10518	T10516	compound	wire,loops
R7068	T10519	T10518	punct	-,wire
R7069	T10521	T10520	auxpass	were,used
R7070	T10522	T10520	prep	as,used
R7071	T10523	T10524	det	the,electrodes
R7072	T10524	T10522	pobj	electrodes,as
R7073	T10525	T10524	amod	active,electrodes
R7074	T10526	T10520	prep	under,used
R7075	T10527	T10528	nummod	0.5,%
R7076	T10528	T10529	nmod	%,hydrochloride
R7077	T10529	T10531	nmod	hydrochloride,anesthesia
R7078	T10530	T10529	nmod	proparacaine,hydrochloride
R7079	T10531	T10526	pobj	anesthesia,under
R7080	T10532	T10531	amod	topical,anesthesia
R7081	T10533	T10520	punct	.,used
R7082	T10535	T10536	amod	Gold,wire
R7083	T10536	T10538	compound	wire,electrodes
R7084	T10537	T10536	punct	-,wire
R7085	T10538	T10539	nsubj	electrodes,served
R7086	T10540	T10538	acl	placed,electrodes
R7087	T10541	T10540	prep	on,placed
R7088	T10542	T10543	det	the,sclera
R7089	T10543	T10541	pobj	sclera,on
R7090	T10544	T10543	prep	near,sclera
R7091	T10545	T10546	det	the,limbus
R7092	T10546	T10544	pobj	limbus,near
R7093	T10547	T10539	prep	as,served
R7094	T10548	T10549	compound	reference,electrodes
R7095	T10549	T10547	pobj	electrodes,as
R7096	T10550	T10539	punct	.,served
R7097	T10552	T10553	det	A,wire
R7098	T10553	T10555	nsubjpass	wire,attached
R7099	T10554	T10553	compound	ground,wire
R7100	T10556	T10555	auxpass	was,attached
R7101	T10557	T10555	prep	to,attached
R7102	T10558	T10559	det	the,ear
R7103	T10559	T10557	pobj	ear,to
R7104	T10560	T10555	punct	.,attached
R7105	T10562	T10563	amod	Scotopic,responses
R7106	T10563	T10564	nsubjpass	responses,recorded
R7107	T10565	T10564	auxpass	were,recorded
R7108	T10566	T10564	prep	from,recorded
R7109	T10567	T10566	pobj	threshold,from
R7110	T10568	T10566	prep	to,from
R7111	T10569	T10570	nummod	9,units
R7112	T10570	T10568	pobj	units,to
R7113	T10571	T10570	compound	log,units
R7114	T10572	T10570	prep	of,units
R7115	T10573	T10572	pobj	intensity,of
R7116	T10574	T10570	prep	above,units
R7117	T10575	T10574	pobj	threshold,above
R7118	T10576	T10564	punct	", ",recorded
R7119	T10577	T10564	conj	amplified,recorded
R7120	T10578	T10577	punct	", ",amplified
R7121	T10579	T10577	cc	and,amplified
R7122	T10580	T10577	conj	filtered,amplified
R7123	T10581	T10582	punct	(,Hz
R7124	T10582	T10580	parataxis	Hz,filtered
R7125	T10583	T10584	nummod	"5,000",gain
R7126	T10584	T10582	dep	gain,Hz
R7127	T10585	T10582	punct	", ",Hz
R7128	T10586	T10587	quantmod	0.1,"1,000"
R7129	T10587	T10582	nummod	"1,000",Hz
R7130	T10588	T10587	punct	–,"1,000"
R7131	T10589	T10582	punct	),Hz
R7132	T10590	T10564	punct	.,recorded
R7133	T10592	T10593	prep	For,used
R7134	T10594	T10595	amod	photopic,recordings
R7135	T10595	T10592	pobj	recordings,For
R7136	T10596	T10597	compound	background,light
R7137	T10597	T10593	nsubjpass	light,used
R7138	T10598	T10597	prep	at,light
R7139	T10599	T10600	nummod	34,cd
R7140	T10600	T10598	pobj	cd,at
R7141	T10601	T10602	punct	/,m2
R7142	T10602	T10600	prep	m2,cd
R7143	T10603	T10593	auxpass	was,used
R7144	T10604	T10605	aux	to,suppress
R7145	T10605	T10593	advcl	suppress,used
R7146	T10606	T10607	compound	rod,function
R7147	T10607	T10605	dobj	function,suppress
R7148	T10608	T10593	punct	.,used

PMC:1626108 / 41174-52203 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

PMC:1626108 / 41174-52203 JSON TXT 5 Projects