| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T2571 |
0-3 |
NN |
denotes |
p53 |
| T2572 |
4-6 |
VBZ |
denotes |
is |
| T2573 |
7-10 |
CD |
denotes |
one |
| T2574 |
11-13 |
IN |
denotes |
of |
| T2575 |
14-17 |
DT |
denotes |
the |
| T2577 |
18-22 |
RBS |
denotes |
most |
| T2578 |
23-29 |
RB |
denotes |
highly |
| T2579 |
30-38 |
JJ |
denotes |
analyzed |
| T2576 |
39-47 |
NN |
denotes |
proteins |
| T2580 |
48-51 |
IN |
denotes |
for |
| T2581 |
52-55 |
DT |
denotes |
the |
| T2583 |
56-60 |
JJ |
denotes |
past |
| T2584 |
61-63 |
CD |
denotes |
25 |
| T2582 |
64-69 |
NNS |
denotes |
years |
| T2585 |
69-70 |
. |
denotes |
. |
| T2586 |
70-232 |
sentence |
denotes |
Studies in cultured cells, often relying on the transfection of plasmids expressing various p53 mutants, have established models to explain how p53 is regulated. |
| T2587 |
71-78 |
NNS |
denotes |
Studies |
| T2589 |
79-81 |
IN |
denotes |
in |
| T2590 |
82-90 |
VBN |
denotes |
cultured |
| T2591 |
91-96 |
NNS |
denotes |
cells |
| T2592 |
96-98 |
, |
denotes |
, |
| T2593 |
98-103 |
RB |
denotes |
often |
| T2594 |
104-111 |
VBG |
denotes |
relying |
| T2595 |
112-114 |
IN |
denotes |
on |
| T2596 |
115-118 |
DT |
denotes |
the |
| T2597 |
119-131 |
NN |
denotes |
transfection |
| T2598 |
132-134 |
IN |
denotes |
of |
| T2599 |
135-143 |
NNS |
denotes |
plasmids |
| T2600 |
144-154 |
VBG |
denotes |
expressing |
| T2601 |
155-162 |
JJ |
denotes |
various |
| T2603 |
163-166 |
NN |
denotes |
p53 |
| T2602 |
167-174 |
NNS |
denotes |
mutants |
| T2604 |
174-176 |
, |
denotes |
, |
| T2605 |
176-180 |
VBD |
denotes |
have |
| T2588 |
181-192 |
VBN |
denotes |
established |
| T2606 |
193-199 |
NNS |
denotes |
models |
| T2607 |
200-202 |
TO |
denotes |
to |
| T2608 |
203-210 |
VB |
denotes |
explain |
| T2609 |
211-214 |
WRB |
denotes |
how |
| T2611 |
215-218 |
NN |
denotes |
p53 |
| T2612 |
219-221 |
VBZ |
denotes |
is |
| T2610 |
222-231 |
VBN |
denotes |
regulated |
| T2613 |
231-232 |
. |
denotes |
. |
| T2614 |
232-422 |
sentence |
denotes |
In recent years, some of these models were tested in vivo by targeting subtle mutations at the p53 locus using homologous recombination in embryonic stem (ES) cells to generate mutant mice. |
| T2615 |
233-235 |
IN |
denotes |
In |
| T2617 |
236-242 |
JJ |
denotes |
recent |
| T2618 |
243-248 |
NNS |
denotes |
years |
| T2619 |
248-250 |
, |
denotes |
, |
| T2620 |
250-254 |
DT |
denotes |
some |
| T2621 |
255-257 |
IN |
denotes |
of |
| T2622 |
258-263 |
DT |
denotes |
these |
| T2623 |
264-270 |
NNS |
denotes |
models |
| T2624 |
271-275 |
VBD |
denotes |
were |
| T2616 |
276-282 |
VBN |
denotes |
tested |
| T2625 |
283-285 |
FW |
denotes |
in |
| T2626 |
286-290 |
FW |
denotes |
vivo |
| T2627 |
291-293 |
IN |
denotes |
by |
| T2628 |
294-303 |
VBG |
denotes |
targeting |
| T2629 |
304-310 |
JJ |
denotes |
subtle |
| T2630 |
311-320 |
NNS |
denotes |
mutations |
| T2631 |
321-323 |
IN |
denotes |
at |
| T2632 |
324-327 |
DT |
denotes |
the |
| T2634 |
328-331 |
NN |
denotes |
p53 |
| T2633 |
332-337 |
NN |
denotes |
locus |
| T2635 |
338-343 |
VBG |
denotes |
using |
| T2636 |
344-354 |
JJ |
denotes |
homologous |
| T2637 |
355-368 |
NN |
denotes |
recombination |
| T2638 |
369-371 |
IN |
denotes |
in |
| T2639 |
372-381 |
JJ |
denotes |
embryonic |
| T2640 |
382-386 |
NN |
denotes |
stem |
| T2642 |
387-388 |
-LRB- |
denotes |
( |
| T2643 |
388-390 |
NN |
denotes |
ES |
| T2644 |
390-391 |
-RRB- |
denotes |
) |
| T2641 |
392-397 |
NNS |
denotes |
cells |
| T2645 |
398-400 |
TO |
denotes |
to |
| T2646 |
401-409 |
VB |
denotes |
generate |
| T2647 |
410-416 |
JJ |
denotes |
mutant |
| T2648 |
417-421 |
NNS |
denotes |
mice |
| T2649 |
421-422 |
. |
denotes |
. |
| T2650 |
422-625 |
sentence |
denotes |
The strength of this approach is that mutations are tested in a genomic setting and expressed from the endogenous promoter, ensuring physiological expression levels and correct spatio-temporal profiles. |
| T2651 |
423-426 |
DT |
denotes |
The |
| T2652 |
427-435 |
NN |
denotes |
strength |
| T2654 |
436-438 |
IN |
denotes |
of |
| T2655 |
439-443 |
DT |
denotes |
this |
| T2656 |
444-452 |
NN |
denotes |
approach |
| T2653 |
453-455 |
VBZ |
denotes |
is |
| T2657 |
456-460 |
IN |
denotes |
that |
| T2659 |
461-470 |
NNS |
denotes |
mutations |
| T2660 |
471-474 |
VBP |
denotes |
are |
| T2658 |
475-481 |
VBN |
denotes |
tested |
| T2661 |
482-484 |
IN |
denotes |
in |
| T2662 |
485-486 |
DT |
denotes |
a |
| T2664 |
487-494 |
JJ |
denotes |
genomic |
| T2663 |
495-502 |
NN |
denotes |
setting |
| T2665 |
503-506 |
CC |
denotes |
and |
| T2666 |
507-516 |
VBN |
denotes |
expressed |
| T2667 |
517-521 |
IN |
denotes |
from |
| T2668 |
522-525 |
DT |
denotes |
the |
| T2670 |
526-536 |
JJ |
denotes |
endogenous |
| T2669 |
537-545 |
NN |
denotes |
promoter |
| T2671 |
545-547 |
, |
denotes |
, |
| T2672 |
547-555 |
VBG |
denotes |
ensuring |
| T2673 |
556-569 |
JJ |
denotes |
physiological |
| T2675 |
570-580 |
NN |
denotes |
expression |
| T2674 |
581-587 |
NNS |
denotes |
levels |
| T2676 |
588-591 |
CC |
denotes |
and |
| T2677 |
592-599 |
JJ |
denotes |
correct |
| T2679 |
600-606 |
JJ |
denotes |
spatio |
| T2681 |
606-607 |
HYPH |
denotes |
- |
| T2680 |
607-615 |
JJ |
denotes |
temporal |
| T2678 |
616-624 |
NNS |
denotes |
profiles |
| T2682 |
624-625 |
. |
denotes |
. |
| T2683 |
625-891 |
sentence |
denotes |
As significant differences between phenotypes from targeted p53 mutants in vivo and transfection data were observed [e.g. Refs (1–5)], more targeted mutations need to be generated and analyzed in multiple tissues to formulate more accurate models of p53 regulation. |
| T2684 |
626-628 |
IN |
denotes |
As |
| T2686 |
629-640 |
JJ |
denotes |
significant |
| T2687 |
641-652 |
NNS |
denotes |
differences |
| T2688 |
653-660 |
IN |
denotes |
between |
| T2689 |
661-671 |
NNS |
denotes |
phenotypes |
| T2690 |
672-676 |
IN |
denotes |
from |
| T2691 |
677-685 |
VBN |
denotes |
targeted |
| T2693 |
686-689 |
NN |
denotes |
p53 |
| T2692 |
690-697 |
NNS |
denotes |
mutants |
| T2694 |
698-700 |
FW |
denotes |
in |
| T2695 |
701-705 |
FW |
denotes |
vivo |
| T2696 |
706-709 |
CC |
denotes |
and |
| T2697 |
710-722 |
NN |
denotes |
transfection |
| T2698 |
723-727 |
NNS |
denotes |
data |
| T2699 |
728-732 |
VBD |
denotes |
were |
| T2685 |
733-741 |
VBN |
denotes |
observed |
| T2701 |
742-743 |
-LRB- |
denotes |
[ |
| T2703 |
743-747 |
FW |
denotes |
e.g. |
| T2704 |
748-752 |
NNS |
denotes |
Refs |
| T2705 |
753-754 |
-LRB- |
denotes |
( |
| T2702 |
754-755 |
CD |
denotes |
1 |
| T2706 |
755-756 |
SYM |
denotes |
– |
| T2707 |
756-757 |
CD |
denotes |
5 |
| T2708 |
757-758 |
-RRB- |
denotes |
) |
| T2709 |
758-759 |
-RRB- |
denotes |
] |
| T2710 |
759-761 |
, |
denotes |
, |
| T2711 |
761-765 |
RBR |
denotes |
more |
| T2712 |
766-774 |
VBN |
denotes |
targeted |
| T2713 |
775-784 |
NNS |
denotes |
mutations |
| T2700 |
785-789 |
VBP |
denotes |
need |
| T2714 |
790-792 |
TO |
denotes |
to |
| T2716 |
793-795 |
VB |
denotes |
be |
| T2715 |
796-805 |
VBN |
denotes |
generated |
| T2717 |
806-809 |
CC |
denotes |
and |
| T2718 |
810-818 |
VBN |
denotes |
analyzed |
| T2719 |
819-821 |
IN |
denotes |
in |
| T2720 |
822-830 |
JJ |
denotes |
multiple |
| T2721 |
831-838 |
NNS |
denotes |
tissues |
| T2722 |
839-841 |
TO |
denotes |
to |
| T2723 |
842-851 |
VB |
denotes |
formulate |
| T2724 |
852-856 |
RBR |
denotes |
more |
| T2725 |
857-865 |
JJ |
denotes |
accurate |
| T2726 |
866-872 |
NNS |
denotes |
models |
| T2727 |
873-875 |
IN |
denotes |
of |
| T2728 |
876-879 |
NN |
denotes |
p53 |
| T2729 |
880-890 |
NN |
denotes |
regulation |
| T2730 |
890-891 |
. |
denotes |
. |
| R204 |
T2571 |
T2572 |
nsubj |
p53,is |
| R205 |
T2573 |
T2572 |
attr |
one,is |
| R206 |
T2574 |
T2573 |
prep |
of,one |
| R207 |
T2575 |
T2576 |
det |
the,proteins |
| R208 |
T2576 |
T2574 |
pobj |
proteins,of |
| R209 |
T2577 |
T2578 |
advmod |
most,highly |
| R210 |
T2578 |
T2579 |
advmod |
highly,analyzed |
| R211 |
T2579 |
T2576 |
amod |
analyzed,proteins |
| R212 |
T2580 |
T2572 |
prep |
for,is |
| R213 |
T2581 |
T2582 |
det |
the,years |
| R214 |
T2582 |
T2580 |
pobj |
years,for |
| R215 |
T2583 |
T2582 |
amod |
past,years |
| R216 |
T2584 |
T2582 |
nummod |
25,years |
| R217 |
T2585 |
T2572 |
punct |
.,is |
| R218 |
T2587 |
T2588 |
nsubj |
Studies,established |
| R219 |
T2589 |
T2587 |
prep |
in,Studies |
| R220 |
T2590 |
T2591 |
amod |
cultured,cells |
| R221 |
T2591 |
T2589 |
pobj |
cells,in |
| R222 |
T2592 |
T2587 |
punct |
", ",Studies |
| R223 |
T2593 |
T2594 |
advmod |
often,relying |
| R224 |
T2594 |
T2587 |
acl |
relying,Studies |
| R225 |
T2595 |
T2594 |
prep |
on,relying |
| R226 |
T2596 |
T2597 |
det |
the,transfection |
| R227 |
T2597 |
T2595 |
pobj |
transfection,on |
| R228 |
T2598 |
T2597 |
prep |
of,transfection |
| R229 |
T2599 |
T2598 |
pobj |
plasmids,of |
| R230 |
T2600 |
T2599 |
acl |
expressing,plasmids |
| R231 |
T2601 |
T2602 |
amod |
various,mutants |
| R232 |
T2602 |
T2600 |
dobj |
mutants,expressing |
| R233 |
T2603 |
T2602 |
compound |
p53,mutants |
| R234 |
T2604 |
T2588 |
punct |
", ",established |
| R235 |
T2605 |
T2588 |
aux |
have,established |
| R236 |
T2606 |
T2588 |
dobj |
models,established |
| R237 |
T2607 |
T2608 |
aux |
to,explain |
| R238 |
T2608 |
T2606 |
advcl |
explain,models |
| R239 |
T2609 |
T2610 |
advmod |
how,regulated |
| R240 |
T2610 |
T2608 |
ccomp |
regulated,explain |
| R241 |
T2611 |
T2610 |
nsubjpass |
p53,regulated |
| R242 |
T2612 |
T2610 |
auxpass |
is,regulated |
| R243 |
T2613 |
T2588 |
punct |
.,established |
| R244 |
T2615 |
T2616 |
prep |
In,tested |
| R245 |
T2617 |
T2618 |
amod |
recent,years |
| R246 |
T2618 |
T2615 |
pobj |
years,In |
| R247 |
T2619 |
T2616 |
punct |
", ",tested |
| R248 |
T2620 |
T2616 |
nsubjpass |
some,tested |
| R249 |
T2621 |
T2620 |
prep |
of,some |
| R250 |
T2622 |
T2623 |
det |
these,models |
| R251 |
T2623 |
T2621 |
pobj |
models,of |
| R252 |
T2624 |
T2616 |
auxpass |
were,tested |
| R253 |
T2625 |
T2626 |
advmod |
in,vivo |
| R254 |
T2626 |
T2616 |
advmod |
vivo,tested |
| R255 |
T2627 |
T2616 |
prep |
by,tested |
| R256 |
T2628 |
T2627 |
pcomp |
targeting,by |
| R257 |
T2629 |
T2630 |
amod |
subtle,mutations |
| R258 |
T2630 |
T2628 |
dobj |
mutations,targeting |
| R259 |
T2631 |
T2628 |
prep |
at,targeting |
| R260 |
T2632 |
T2633 |
det |
the,locus |
| R261 |
T2633 |
T2631 |
pobj |
locus,at |
| R262 |
T2695 |
T2690 |
advmod |
vivo,from |
| R263 |
T2634 |
T2633 |
compound |
p53,locus |
| R264 |
T2696 |
T2689 |
cc |
and,phenotypes |
| R265 |
T2635 |
T2628 |
advcl |
using,targeting |
| R266 |
T2697 |
T2698 |
compound |
transfection,data |
| R267 |
T2698 |
T2689 |
conj |
data,phenotypes |
| R268 |
T2636 |
T2637 |
amod |
homologous,recombination |
| R269 |
T2699 |
T2685 |
auxpass |
were,observed |
| R270 |
T2637 |
T2635 |
dobj |
recombination,using |
| R271 |
T2701 |
T2702 |
punct |
[,1 |
| R272 |
T2702 |
T2685 |
parataxis |
1,observed |
| R273 |
T2703 |
T2702 |
advmod |
e.g.,1 |
| R274 |
T2638 |
T2635 |
prep |
in,using |
| R275 |
T2704 |
T2702 |
nmod |
Refs,1 |
| R276 |
T2705 |
T2702 |
punct |
(,1 |
| R277 |
T2639 |
T2640 |
amod |
embryonic,stem |
| R278 |
T2706 |
T2707 |
punct |
–,5 |
| R279 |
T2707 |
T2702 |
prep |
5,1 |
| R280 |
T2708 |
T2702 |
punct |
),1 |
| R281 |
T2640 |
T2641 |
nmod |
stem,cells |
| R282 |
T2709 |
T2702 |
punct |
],1 |
| R283 |
T2641 |
T2638 |
pobj |
cells,in |
| R284 |
T2710 |
T2700 |
punct |
", ",need |
| R285 |
T2642 |
T2640 |
punct |
(,stem |
| R286 |
T2711 |
T2712 |
advmod |
more,targeted |
| R287 |
T2712 |
T2713 |
amod |
targeted,mutations |
| R288 |
T2643 |
T2640 |
appos |
ES,stem |
| R289 |
T2713 |
T2700 |
nsubj |
mutations,need |
| R290 |
T2714 |
T2715 |
aux |
to,generated |
| R291 |
T2715 |
T2700 |
xcomp |
generated,need |
| R292 |
T2716 |
T2715 |
auxpass |
be,generated |
| R293 |
T2644 |
T2641 |
punct |
),cells |
| R294 |
T2717 |
T2715 |
cc |
and,generated |
| R295 |
T2718 |
T2715 |
conj |
analyzed,generated |
| R296 |
T2645 |
T2646 |
aux |
to,generate |
| R297 |
T2719 |
T2718 |
prep |
in,analyzed |
| R298 |
T2720 |
T2721 |
amod |
multiple,tissues |
| R299 |
T2721 |
T2719 |
pobj |
tissues,in |
| R300 |
T2722 |
T2723 |
aux |
to,formulate |
| R301 |
T2723 |
T2718 |
advcl |
formulate,analyzed |
| R302 |
T2724 |
T2725 |
advmod |
more,accurate |
| R303 |
T2646 |
T2635 |
advcl |
generate,using |
| R304 |
T2725 |
T2726 |
amod |
accurate,models |
| R305 |
T2647 |
T2648 |
amod |
mutant,mice |
| R306 |
T2726 |
T2723 |
dobj |
models,formulate |
| R307 |
T2727 |
T2726 |
prep |
of,models |
| R308 |
T2728 |
T2729 |
compound |
p53,regulation |
| R309 |
T2729 |
T2727 |
pobj |
regulation,of |
| R310 |
T2730 |
T2700 |
punct |
.,need |
| R311 |
T2648 |
T2646 |
dobj |
mice,generate |
| R313 |
T2649 |
T2616 |
punct |
.,tested |
| R316 |
T2651 |
T2652 |
det |
The,strength |
| R318 |
T2652 |
T2653 |
nsubj |
strength,is |
| R321 |
T2654 |
T2652 |
prep |
of,strength |
| R325 |
T2655 |
T2656 |
det |
this,approach |
| R330 |
T2656 |
T2654 |
pobj |
approach,of |
| R334 |
T2657 |
T2658 |
mark |
that,tested |
| R337 |
T2658 |
T2653 |
ccomp |
tested,is |
| R341 |
T2659 |
T2658 |
nsubj |
mutations,tested |
| R346 |
T2660 |
T2658 |
aux |
are,tested |
| R350 |
T2661 |
T2658 |
prep |
in,tested |
| R357 |
T2662 |
T2663 |
det |
a,setting |
| R360 |
T2663 |
T2661 |
pobj |
setting,in |
| R365 |
T2664 |
T2663 |
amod |
genomic,setting |
| R368 |
T2665 |
T2658 |
cc |
and,tested |
| R372 |
T2666 |
T2658 |
conj |
expressed,tested |
| R376 |
T2667 |
T2666 |
prep |
from,expressed |
| R380 |
T2668 |
T2669 |
det |
the,promoter |
| R382 |
T2669 |
T2667 |
pobj |
promoter,from |
| R385 |
T2670 |
T2669 |
amod |
endogenous,promoter |
| R390 |
T2671 |
T2658 |
punct |
", ",tested |
| R394 |
T2672 |
T2658 |
advcl |
ensuring,tested |
| R398 |
T2673 |
T2674 |
amod |
physiological,levels |
| R401 |
T2674 |
T2672 |
dobj |
levels,ensuring |
| R404 |
T2675 |
T2674 |
compound |
expression,levels |
| R405 |
T2676 |
T2674 |
cc |
and,levels |
| R407 |
T2677 |
T2678 |
amod |
correct,profiles |
| R410 |
T2678 |
T2674 |
conj |
profiles,levels |
| R414 |
T2679 |
T2680 |
amod |
spatio,temporal |
| R417 |
T2680 |
T2678 |
amod |
temporal,profiles |
| R421 |
T2681 |
T2680 |
punct |
-,temporal |
| R425 |
T2682 |
T2653 |
punct |
.,is |
| R428 |
T2684 |
T2685 |
mark |
As,observed |
| R434 |
T2685 |
T2700 |
advcl |
observed,need |
| R438 |
T2686 |
T2687 |
amod |
significant,differences |
| R440 |
T2687 |
T2685 |
nsubjpass |
differences,observed |
| R443 |
T2688 |
T2687 |
prep |
between,differences |
| R447 |
T2689 |
T2688 |
pobj |
phenotypes,between |
| R451 |
T2690 |
T2689 |
prep |
from,phenotypes |
| R455 |
T2691 |
T2692 |
amod |
targeted,mutants |
| R461 |
T2692 |
T2690 |
pobj |
mutants,from |
| R463 |
T2693 |
T2692 |
compound |
p53,mutants |
| R467 |
T2694 |
T2695 |
advmod |
in,vivo |
