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PMC:1448208 / 14017-15073 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T8110 0-6 NNS denotes Livers
T8112 7-11 VBD denotes were
T8111 12-19 VBN denotes removed
T8113 19-21 , denotes ,
T8114 21-27 VBN denotes frozen
T8115 28-30 IN denotes in
T8116 31-37 JJ denotes liquid
T8117 38-46 NN denotes nitrogen
T8118 46-48 , denotes ,
T8119 48-51 CC denotes and
T8120 52-58 VBN denotes stored
T8121 59-61 IN denotes at
T8122 62-63 HYPH denotes -
T8123 63-65 CD denotes 80
T8124 65-67 NN denotes °C
T8125 67-68 . denotes .
T8126 68-187 sentence denotes At the time of enzyme assay, livers were thawed, and homogenized (1:9, w/v) in 0.25 M sucrose, 10 mM Tris-HC1, pH 7.4.
T8127 69-71 IN denotes At
T8129 72-75 DT denotes the
T8130 76-80 NN denotes time
T8131 81-83 IN denotes of
T8132 84-90 NN denotes enzyme
T8133 91-96 NN denotes assay
T8134 96-98 , denotes ,
T8135 98-104 NNS denotes livers
T8136 105-109 VBD denotes were
T8128 110-116 VBN denotes thawed
T8137 116-118 , denotes ,
T8138 118-121 CC denotes and
T8139 122-133 VBN denotes homogenized
T8140 134-135 -LRB- denotes (
T8142 135-136 CD denotes 1
T8144 136-137 SYM denotes :
T8143 137-138 CD denotes 9
T8145 138-140 , denotes ,
T8146 140-141 NN denotes w
T8147 141-142 HYPH denotes /
T8141 142-143 NN denotes v
T8148 143-144 -RRB- denotes )
T8149 145-147 IN denotes in
T8150 148-152 CD denotes 0.25
T8151 153-154 NN denotes M
T8152 155-162 NN denotes sucrose
T8153 162-164 , denotes ,
T8154 164-166 CD denotes 10
T8155 167-169 NN denotes mM
T8157 170-174 NN denotes Tris
T8158 174-175 HYPH denotes -
T8156 175-178 NN denotes HC1
T8159 178-180 , denotes ,
T8160 180-182 NN denotes pH
T8161 183-186 CD denotes 7.4
T8162 186-187 . denotes .
T8163 187-323 sentence denotes Liver homogenates were centrifuged at 600 × g for 10 min at 4°C and the supernatant fraction was saved to determine the BCKDH activity.
T8164 188-193 NN denotes Liver
T8165 194-205 NNS denotes homogenates
T8167 206-210 VBD denotes were
T8166 211-222 VBN denotes centrifuged
T8168 223-225 IN denotes at
T8169 226-229 CD denotes 600
T8171 230-231 SYM denotes ×
T8170 232-233 NN denotes g
T8172 234-237 IN denotes for
T8173 238-240 CD denotes 10
T8174 241-244 NN denotes min
T8175 245-247 IN denotes at
T8176 248-249 CD denotes 4
T8177 249-251 NN denotes °C
T8178 252-255 CC denotes and
T8179 256-259 DT denotes the
T8181 260-271 NN denotes supernatant
T8180 272-280 NN denotes fraction
T8183 281-284 VBD denotes was
T8182 285-290 VBN denotes saved
T8184 291-293 TO denotes to
T8185 294-303 VB denotes determine
T8186 304-307 DT denotes the
T8188 308-313 NN denotes BCKDH
T8187 314-322 NN denotes activity
T8189 322-323 . denotes .
T8190 323-447 sentence denotes The use of tissue homogenates was necessitated by the limited availability of liver tissue, particularly from newborn pups.
T8191 324-327 DT denotes The
T8192 328-331 NN denotes use
T8194 332-334 IN denotes of
T8195 335-341 NN denotes tissue
T8196 342-353 NNS denotes homogenates
T8197 354-357 VBD denotes was
T8193 358-370 VBN denotes necessitated
T8198 371-373 IN denotes by
T8199 374-377 DT denotes the
T8201 378-385 JJ denotes limited
T8200 386-398 NN denotes availability
T8202 399-401 IN denotes of
T8203 402-407 NN denotes liver
T8204 408-414 NN denotes tissue
T8205 414-416 , denotes ,
T8206 416-428 RB denotes particularly
T8207 429-433 IN denotes from
T8208 434-441 JJ denotes newborn
T8209 442-446 NNS denotes pups
T8210 446-447 . denotes .
T8211 447-573 sentence denotes BCKDH activity was determined by measuring the release of 14CO2 from α-keto [1-14C] isocaproate as described previously [31].
T8212 448-453 NN denotes BCKDH
T8213 454-462 NN denotes activity
T8215 463-466 VBD denotes was
T8214 467-477 VBN denotes determined
T8216 478-480 IN denotes by
T8217 481-490 VBG denotes measuring
T8218 491-494 DT denotes the
T8219 495-502 NN denotes release
T8220 503-505 IN denotes of
T8221 506-511 NN denotes 14CO2
T8222 512-516 IN denotes from
T8223 517-518 NN denotes α
T8225 518-519 HYPH denotes -
T8224 519-523 NN denotes keto
T8227 524-525 -LRB- denotes [
T8228 525-526 CD denotes 1
T8229 526-527 SYM denotes -
T8230 527-530 NN denotes 14C
T8231 530-531 -RRB- denotes ]
T8226 532-543 NN denotes isocaproate
T8232 544-546 IN denotes as
T8233 547-556 VBN denotes described
T8234 557-567 RB denotes previously
T8235 568-569 -LRB- denotes [
T8236 569-571 CD denotes 31
T8237 571-572 -RRB- denotes ]
T8238 572-573 . denotes .
T8239 573-898 sentence denotes The complete reaction mixture contained (final volume 1 ml) 30 mM potassium phosphate buffer, pH 7.4, 0.20 mM α-ketoisocaproate, 0.40 mM CoASH, 0.40 mM thiamin pyrophosphate, 2 mM NAD+, 2 mM dithiothreitol, 5 mM Mg2+, approximately 250,000 DPM of α-keto [1-14C] isocaproate, and 0.10 ml of liver homogenate (2–3 mg protein).
T8240 574-577 DT denotes The
T8242 578-586 JJ denotes complete
T8243 587-595 NN denotes reaction
T8241 596-603 NN denotes mixture
T8244 604-613 VBD denotes contained
T8245 614-615 -LRB- denotes (
T8247 615-620 JJ denotes final
T8246 621-627 NN denotes volume
T8248 628-629 CD denotes 1
T8249 630-632 NN denotes ml
T8250 632-633 -RRB- denotes )
T8251 634-636 CD denotes 30
T8252 637-639 NN denotes mM
T8254 640-649 NN denotes potassium
T8255 650-659 NN denotes phosphate
T8253 660-666 NN denotes buffer
T8256 666-668 , denotes ,
T8257 668-670 NN denotes pH
T8258 671-674 CD denotes 7.4
T8259 674-676 , denotes ,
T8260 676-680 CD denotes 0.20
T8261 681-683 NN denotes mM
T8263 684-685 NN denotes α
T8264 685-686 HYPH denotes -
T8262 686-701 NN denotes ketoisocaproate
T8265 701-703 , denotes ,
T8266 703-707 CD denotes 0.40
T8267 708-710 NN denotes mM
T8268 711-716 NN denotes CoASH
T8269 716-718 , denotes ,
T8270 718-722 CD denotes 0.40
T8271 723-725 NN denotes mM
T8273 726-733 NN denotes thiamin
T8272 734-747 NN denotes pyrophosphate
T8274 747-749 , denotes ,
T8275 749-750 CD denotes 2
T8276 751-753 NN denotes mM
T8277 754-757 NN denotes NAD
T8278 757-758 SYM denotes +
T8279 758-760 , denotes ,
T8280 760-761 CD denotes 2
T8281 762-764 NN denotes mM
T8282 765-779 NN denotes dithiothreitol
T8283 779-781 , denotes ,
T8284 781-782 CD denotes 5
T8285 783-785 NN denotes mM
T8286 786-789 NN denotes Mg2
T8287 789-790 SYM denotes +
T8288 790-792 , denotes ,
T8289 792-805 RB denotes approximately
T8290 806-813 CD denotes 250,000
T8291 814-817 NN denotes DPM
T8292 818-820 IN denotes of
T8293 821-822 NN denotes α
T8295 822-823 HYPH denotes -
T8294 823-827 NN denotes keto
T8297 828-829 -LRB- denotes [
T8298 829-830 CD denotes 1
T8299 830-831 SYM denotes -
T8300 831-834 NN denotes 14C
T8301 834-835 -RRB- denotes ]
T8296 836-847 NN denotes isocaproate
T8302 847-849 , denotes ,
T8303 849-852 CC denotes and
T8304 853-857 CD denotes 0.10
T8305 858-860 NN denotes ml
T8306 861-863 IN denotes of
T8307 864-869 NN denotes liver
T8308 870-880 NN denotes homogenate
T8309 881-882 -LRB- denotes (
T8311 882-883 CD denotes 2
T8313 883-884 SYM denotes –
T8312 884-885 CD denotes 3
T8314 886-888 NN denotes mg
T8310 889-896 NN denotes protein
T8315 896-897 -RRB- denotes )
T8316 897-898 . denotes .
T8317 898-1056 sentence denotes Assays were carried out for 15 min at 37°C, 14CO2 was trapped in hydroxide of Hyamine, and radioactivity was determined by liquid scintillation spectrometry.
T8318 899-905 NNS denotes Assays
T8320 906-910 VBD denotes were
T8319 911-918 VBN denotes carried
T8321 919-922 RP denotes out
T8322 923-926 IN denotes for
T8323 927-929 CD denotes 15
T8324 930-933 NN denotes min
T8325 934-936 IN denotes at
T8326 937-939 CD denotes 37
T8327 939-941 NN denotes °C
T8328 941-943 , denotes ,
T8329 943-948 NN denotes 14CO2
T8331 949-952 VBD denotes was
T8330 953-960 VBN denotes trapped
T8332 961-963 IN denotes in
T8333 964-973 NN denotes hydroxide
T8334 974-976 IN denotes of
T8335 977-984 NN denotes Hyamine
T8336 984-986 , denotes ,
T8337 986-989 CC denotes and
T8338 990-1003 NN denotes radioactivity
T8340 1004-1007 VBD denotes was
T8339 1008-1018 VBN denotes determined
T8341 1019-1021 IN denotes by
T8342 1022-1028 JJ denotes liquid
T8343 1029-1042 NN denotes scintillation
T8344 1043-1055 NN denotes spectrometry
T8345 1055-1056 . denotes .
R2285 T8110 T8111 nsubjpass Livers,removed
R2286 T8112 T8111 auxpass were,removed
R2287 T8113 T8111 punct ", ",removed
R2288 T8114 T8111 conj frozen,removed
R2289 T8115 T8114 prep in,frozen
R2290 T8116 T8117 amod liquid,nitrogen
R2291 T8117 T8115 pobj nitrogen,in
R2292 T8118 T8114 punct ", ",frozen
R2293 T8119 T8114 cc and,frozen
R2294 T8120 T8114 conj stored,frozen
R2295 T8121 T8120 prep at,stored
R2296 T8122 T8123 punct -,80
R2297 T8123 T8124 nummod 80,°C
R2298 T8124 T8121 pobj °C,at
R2299 T8125 T8111 punct .,removed
R2300 T8127 T8128 prep At,thawed
R2301 T8129 T8130 det the,time
R2302 T8130 T8127 pobj time,At
R2303 T8131 T8130 prep of,time
R2304 T8132 T8133 compound enzyme,assay
R2305 T8133 T8131 pobj assay,of
R2306 T8134 T8128 punct ", ",thawed
R2307 T8135 T8128 nsubjpass livers,thawed
R2308 T8136 T8128 auxpass were,thawed
R2309 T8137 T8128 punct ", ",thawed
R2310 T8138 T8128 cc and,thawed
R2311 T8139 T8128 conj homogenized,thawed
R2312 T8140 T8141 punct (,v
R2313 T8141 T8139 parataxis v,homogenized
R2314 T8142 T8143 nummod 1,9
R2315 T8143 T8141 dep 9,v
R2316 T8144 T8143 punct :,9
R2317 T8145 T8141 punct ", ",v
R2318 T8146 T8141 compound w,v
R2319 T8147 T8141 punct /,v
R2320 T8148 T8141 punct ),v
R2321 T8149 T8139 prep in,homogenized
R2322 T8150 T8151 nummod 0.25,M
R2323 T8151 T8152 compound M,sucrose
R2324 T8152 T8149 pobj sucrose,in
R2325 T8153 T8152 punct ", ",sucrose
R2326 T8154 T8155 nummod 10,mM
R2327 T8155 T8156 compound mM,HC1
R2328 T8156 T8152 appos HC1,sucrose
R2329 T8157 T8156 compound Tris,HC1
R2330 T8158 T8156 punct -,HC1
R2331 T8159 T8156 punct ", ",HC1
R2332 T8160 T8156 npadvmod pH,HC1
R2333 T8161 T8160 nummod 7.4,pH
R2334 T8162 T8128 punct .,thawed
R2335 T8164 T8165 compound Liver,homogenates
R2336 T8165 T8166 nsubjpass homogenates,centrifuged
R2337 T8167 T8166 auxpass were,centrifuged
R2338 T8168 T8166 prep at,centrifuged
R2339 T8169 T8170 nummod 600,g
R2340 T8170 T8168 pobj g,at
R2341 T8171 T8169 punct ×,600
R2342 T8172 T8166 prep for,centrifuged
R2343 T8173 T8174 nummod 10,min
R2344 T8174 T8172 pobj min,for
R2345 T8175 T8166 prep at,centrifuged
R2346 T8176 T8177 nummod 4,°C
R2347 T8177 T8175 pobj °C,at
R2348 T8178 T8166 cc and,centrifuged
R2349 T8179 T8180 det the,fraction
R2350 T8180 T8182 nsubjpass fraction,saved
R2351 T8181 T8180 compound supernatant,fraction
R2352 T8182 T8166 conj saved,centrifuged
R2353 T8183 T8182 auxpass was,saved
R2354 T8184 T8185 aux to,determine
R2355 T8185 T8182 advcl determine,saved
R2356 T8186 T8187 det the,activity
R2357 T8187 T8185 dobj activity,determine
R2358 T8188 T8187 compound BCKDH,activity
R2359 T8189 T8182 punct .,saved
R2360 T8191 T8192 det The,use
R2361 T8192 T8193 nsubjpass use,necessitated
R2362 T8194 T8192 prep of,use
R2363 T8195 T8196 compound tissue,homogenates
R2364 T8196 T8194 pobj homogenates,of
R2365 T8197 T8193 auxpass was,necessitated
R2366 T8198 T8193 agent by,necessitated
R2367 T8199 T8200 det the,availability
R2368 T8200 T8198 pobj availability,by
R2369 T8201 T8200 amod limited,availability
R2370 T8202 T8200 prep of,availability
R2371 T8203 T8204 compound liver,tissue
R2372 T8204 T8202 pobj tissue,of
R2373 T8205 T8204 punct ", ",tissue
R2374 T8206 T8207 advmod particularly,from
R2375 T8207 T8204 prep from,tissue
R2376 T8208 T8209 amod newborn,pups
R2377 T8209 T8207 pobj pups,from
R2378 T8210 T8193 punct .,necessitated
R2379 T8212 T8213 compound BCKDH,activity
R2380 T8213 T8214 nsubjpass activity,determined
R2381 T8215 T8214 auxpass was,determined
R2382 T8216 T8214 prep by,determined
R2383 T8217 T8216 pcomp measuring,by
R2384 T8218 T8219 det the,release
R2385 T8219 T8217 dobj release,measuring
R2386 T8220 T8219 prep of,release
R2387 T8221 T8220 pobj 14CO2,of
R2388 T8222 T8217 prep from,measuring
R2389 T8223 T8224 nmod α,keto
R2390 T8224 T8226 nmod keto,isocaproate
R2391 T8225 T8224 punct -,keto
R2392 T8226 T8222 pobj isocaproate,from
R2393 T8227 T8228 punct [,1
R2394 T8228 T8224 parataxis 1,keto
R2395 T8229 T8230 punct -,14C
R2396 T8230 T8228 prep 14C,1
R2397 T8231 T8228 punct ],1
R2398 T8232 T8233 mark as,described
R2399 T8233 T8214 advcl described,determined
R2400 T8234 T8233 advmod previously,described
R2401 T8235 T8236 punct [,31
R2402 T8236 T8233 parataxis 31,described
R2403 T8237 T8236 punct ],31
R2404 T8238 T8214 punct .,determined
R2405 T8240 T8241 det The,mixture
R2406 T8241 T8244 nsubj mixture,contained
R2407 T8242 T8241 amod complete,mixture
R2408 T8243 T8241 compound reaction,mixture
R2409 T8245 T8246 punct (,volume
R2410 T8246 T8244 parataxis volume,contained
R2411 T8247 T8246 amod final,volume
R2412 T8248 T8249 nummod 1,ml
R2413 T8249 T8246 appos ml,volume
R2414 T8250 T8246 punct ),volume
R2415 T8251 T8252 nummod 30,mM
R2416 T8252 T8253 compound mM,buffer
R2417 T8253 T8244 dobj buffer,contained
R2418 T8254 T8255 compound potassium,phosphate
R2419 T8255 T8253 compound phosphate,buffer
R2420 T8256 T8253 punct ", ",buffer
R2421 T8257 T8253 npadvmod pH,buffer
R2422 T8258 T8257 nummod 7.4,pH
R2423 T8259 T8253 punct ", ",buffer
R2424 T8260 T8261 nummod 0.20,mM
R2425 T8261 T8262 compound mM,ketoisocaproate
R2426 T8262 T8253 conj ketoisocaproate,buffer
R2427 T8263 T8262 compound α,ketoisocaproate
R2428 T8264 T8262 punct -,ketoisocaproate
R2429 T8265 T8262 punct ", ",ketoisocaproate
R2430 T8266 T8267 nummod 0.40,mM
R2431 T8267 T8268 compound mM,CoASH
R2432 T8268 T8262 conj CoASH,ketoisocaproate
R2433 T8269 T8268 punct ", ",CoASH
R2434 T8270 T8271 nummod 0.40,mM
R2435 T8271 T8272 compound mM,pyrophosphate
R2436 T8272 T8268 conj pyrophosphate,CoASH
R2437 T8273 T8272 compound thiamin,pyrophosphate
R2438 T8274 T8272 punct ", ",pyrophosphate
R2439 T8275 T8276 nummod 2,mM
R2440 T8276 T8277 compound mM,NAD
R2441 T8277 T8272 conj NAD,pyrophosphate
R2442 T8278 T8277 punct +,NAD
R2443 T8279 T8277 punct ", ",NAD
R2444 T8280 T8281 nummod 2,mM
R2445 T8281 T8282 compound mM,dithiothreitol
R2446 T8282 T8277 conj dithiothreitol,NAD
R2447 T8283 T8282 punct ", ",dithiothreitol
R2448 T8284 T8285 nummod 5,mM
R2449 T8285 T8286 compound mM,Mg2
R2450 T8286 T8282 conj Mg2,dithiothreitol
R2451 T8287 T8286 punct +,Mg2
R2452 T8288 T8286 punct ", ",Mg2
R2453 T8289 T8290 advmod approximately,"250,000"
R2454 T8290 T8291 nummod "250,000",DPM
R2455 T8291 T8286 conj DPM,Mg2
R2456 T8292 T8291 prep of,DPM
R2457 T8293 T8294 nmod α,keto
R2458 T8294 T8296 nmod keto,isocaproate
R2459 T8295 T8294 punct -,keto
R2460 T8296 T8292 pobj isocaproate,of
R2461 T8297 T8298 punct [,1
R2462 T8298 T8294 parataxis 1,keto
R2463 T8299 T8300 punct -,14C
R2464 T8300 T8298 prep 14C,1
R2465 T8301 T8298 punct ],1
R2466 T8302 T8291 punct ", ",DPM
R2467 T8303 T8291 cc and,DPM
R2468 T8304 T8305 nummod 0.10,ml
R2469 T8305 T8291 conj ml,DPM
R2470 T8306 T8305 prep of,ml
R2471 T8307 T8308 compound liver,homogenate
R2472 T8308 T8306 pobj homogenate,of
R2473 T8309 T8310 punct (,protein
R2474 T8310 T8308 parataxis protein,homogenate
R2475 T8311 T8312 quantmod 2,3
R2476 T8312 T8314 nummod 3,mg
R2477 T8313 T8312 punct –,3
R2478 T8314 T8310 compound mg,protein
R2479 T8315 T8310 punct ),protein
R2480 T8316 T8244 punct .,contained
R2481 T8318 T8319 nsubjpass Assays,carried
R2482 T8320 T8319 auxpass were,carried
R2483 T8321 T8319 prt out,carried
R2484 T8322 T8319 prep for,carried
R2485 T8323 T8324 nummod 15,min
R2486 T8324 T8322 pobj min,for
R2487 T8325 T8319 prep at,carried
R2488 T8326 T8327 nummod 37,°C
R2489 T8327 T8325 pobj °C,at
R2490 T8328 T8319 punct ", ",carried
R2491 T8329 T8330 nsubjpass 14CO2,trapped
R2492 T8330 T8319 conj trapped,carried
R2493 T8331 T8330 auxpass was,trapped
R2494 T8332 T8330 prep in,trapped
R2495 T8333 T8332 pobj hydroxide,in
R2496 T8334 T8333 prep of,hydroxide
R2497 T8335 T8334 pobj Hyamine,of
R2498 T8336 T8330 punct ", ",trapped
R2499 T8337 T8330 cc and,trapped
R2500 T8338 T8339 nsubjpass radioactivity,determined
R2501 T8339 T8330 conj determined,trapped
R2502 T8340 T8339 auxpass was,determined
R2503 T8341 T8339 prep by,determined
R2504 T8342 T8343 amod liquid,scintillation
R2505 T8343 T8344 compound scintillation,spectrometry
R2506 T8344 T8341 pobj spectrometry,by
R2507 T8345 T8339 punct .,determined