| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T31407 |
10-13 |
DT |
denotes |
The |
| T31409 |
14-16 |
NN |
denotes |
mr |
| T31411 |
16-17 |
HYPH |
denotes |
- |
| T31410 |
17-18 |
NN |
denotes |
s |
| T31408 |
19-26 |
NN |
denotes |
protein |
| T31413 |
27-30 |
MD |
denotes |
can |
| T31414 |
31-35 |
NN |
denotes |
self |
| T31415 |
35-36 |
HYPH |
denotes |
- |
| T31412 |
36-45 |
VB |
denotes |
associate |
| T31416 |
46-48 |
IN |
denotes |
in |
| T31417 |
49-58 |
JJ |
denotes |
mammalian |
| T31418 |
59-64 |
NNS |
denotes |
cells |
| T31419 |
64-65 |
. |
denotes |
. |
| T31420 |
65-141 |
sentence |
denotes |
(A) Schematic drawing of the constructs used for immunoprecipitation assay. |
| T31421 |
66-67 |
-LRB- |
denotes |
( |
| T31422 |
67-68 |
LS |
denotes |
A |
| T31424 |
68-69 |
-RRB- |
denotes |
) |
| T31425 |
70-79 |
JJ |
denotes |
Schematic |
| T31423 |
80-87 |
NN |
denotes |
drawing |
| T31426 |
88-90 |
IN |
denotes |
of |
| T31427 |
91-94 |
DT |
denotes |
the |
| T31428 |
95-105 |
NNS |
denotes |
constructs |
| T31429 |
106-110 |
VBN |
denotes |
used |
| T31430 |
111-114 |
IN |
denotes |
for |
| T31431 |
115-134 |
NN |
denotes |
immunoprecipitation |
| T31432 |
135-140 |
NN |
denotes |
assay |
| T31433 |
140-141 |
. |
denotes |
. |
| T31434 |
141-319 |
sentence |
denotes |
HA-tagged or Flag-tagged full-length (amino acids 1 to 542), ΔSAM (amino acids 1 to 400) and SAM (amino acids 401 to 542) regions were inserted into pcDNA3 vector, respectively. |
| T31435 |
142-144 |
NN |
denotes |
HA |
| T31437 |
144-145 |
HYPH |
denotes |
- |
| T31436 |
145-151 |
VBN |
denotes |
tagged |
| T31439 |
152-154 |
CC |
denotes |
or |
| T31440 |
155-159 |
NN |
denotes |
Flag |
| T31442 |
159-160 |
HYPH |
denotes |
- |
| T31441 |
160-166 |
VBN |
denotes |
tagged |
| T31443 |
167-171 |
JJ |
denotes |
full |
| T31445 |
171-172 |
HYPH |
denotes |
- |
| T31444 |
172-178 |
NN |
denotes |
length |
| T31446 |
179-180 |
-LRB- |
denotes |
( |
| T31448 |
180-185 |
NN |
denotes |
amino |
| T31449 |
186-191 |
NNS |
denotes |
acids |
| T31447 |
192-193 |
CD |
denotes |
1 |
| T31450 |
194-196 |
IN |
denotes |
to |
| T31451 |
197-200 |
CD |
denotes |
542 |
| T31452 |
200-201 |
-RRB- |
denotes |
) |
| T31453 |
201-203 |
, |
denotes |
, |
| T31454 |
203-207 |
NN |
denotes |
ΔSAM |
| T31455 |
208-209 |
-LRB- |
denotes |
( |
| T31457 |
209-214 |
NN |
denotes |
amino |
| T31458 |
215-220 |
NNS |
denotes |
acids |
| T31456 |
221-222 |
CD |
denotes |
1 |
| T31459 |
223-225 |
IN |
denotes |
to |
| T31460 |
226-229 |
CD |
denotes |
400 |
| T31461 |
229-230 |
-RRB- |
denotes |
) |
| T31462 |
231-234 |
CC |
denotes |
and |
| T31463 |
235-238 |
NN |
denotes |
SAM |
| T31464 |
239-240 |
-LRB- |
denotes |
( |
| T31466 |
240-245 |
NN |
denotes |
amino |
| T31467 |
246-251 |
NNS |
denotes |
acids |
| T31465 |
252-255 |
CD |
denotes |
401 |
| T31468 |
256-258 |
IN |
denotes |
to |
| T31469 |
259-262 |
CD |
denotes |
542 |
| T31470 |
262-263 |
-RRB- |
denotes |
) |
| T31438 |
264-271 |
NNS |
denotes |
regions |
| T31472 |
272-276 |
VBD |
denotes |
were |
| T31471 |
277-285 |
VBN |
denotes |
inserted |
| T31473 |
286-290 |
IN |
denotes |
into |
| T31474 |
291-297 |
NN |
denotes |
pcDNA3 |
| T31475 |
298-304 |
NN |
denotes |
vector |
| T31476 |
304-306 |
, |
denotes |
, |
| T31477 |
306-318 |
RB |
denotes |
respectively |
| T31478 |
318-319 |
. |
denotes |
. |
| T31479 |
319-391 |
sentence |
denotes |
(B) The constructs indicated above were transfected into HEK293T cells. |
| T31480 |
320-321 |
-LRB- |
denotes |
( |
| T31481 |
321-322 |
LS |
denotes |
B |
| T31483 |
322-323 |
-RRB- |
denotes |
) |
| T31484 |
324-327 |
DT |
denotes |
The |
| T31485 |
328-338 |
NNS |
denotes |
constructs |
| T31486 |
339-348 |
VBN |
denotes |
indicated |
| T31487 |
349-354 |
RB |
denotes |
above |
| T31488 |
355-359 |
VBD |
denotes |
were |
| T31482 |
360-371 |
VBN |
denotes |
transfected |
| T31489 |
372-376 |
IN |
denotes |
into |
| T31490 |
377-384 |
NN |
denotes |
HEK293T |
| T31491 |
385-390 |
NNS |
denotes |
cells |
| T31492 |
390-391 |
. |
denotes |
. |
| T31493 |
391-483 |
sentence |
denotes |
Each lane was co-immunoprecipitated by anti-Flag antibody and detected by anti-HA antibody. |
| T31494 |
392-396 |
DT |
denotes |
Each |
| T31495 |
397-401 |
NN |
denotes |
lane |
| T31497 |
402-405 |
VBD |
denotes |
was |
| T31496 |
406-427 |
VBN |
denotes |
co-immunoprecipitated |
| T31498 |
428-430 |
IN |
denotes |
by |
| T31499 |
431-440 |
JJ |
denotes |
anti-Flag |
| T31500 |
441-449 |
NN |
denotes |
antibody |
| T31501 |
450-453 |
CC |
denotes |
and |
| T31502 |
454-462 |
VBN |
denotes |
detected |
| T31503 |
463-465 |
IN |
denotes |
by |
| T31504 |
466-473 |
JJ |
denotes |
anti-HA |
| T31505 |
474-482 |
NN |
denotes |
antibody |
| T31506 |
482-483 |
. |
denotes |
. |
| T31507 |
483-536 |
sentence |
denotes |
Input protein lysates are shown in the lower panels. |
| T31508 |
484-489 |
NN |
denotes |
Input |
| T31510 |
490-497 |
NN |
denotes |
protein |
| T31509 |
498-505 |
NNS |
denotes |
lysates |
| T31512 |
506-509 |
VBP |
denotes |
are |
| T31511 |
510-515 |
VBN |
denotes |
shown |
| T31513 |
516-518 |
IN |
denotes |
in |
| T31514 |
519-522 |
DT |
denotes |
the |
| T31516 |
523-528 |
JJ |
denotes |
lower |
| T31515 |
529-535 |
NNS |
denotes |
panels |
| T31517 |
535-536 |
. |
denotes |
. |
| T31518 |
536-658 |
sentence |
denotes |
(C) Flag-tagged two site-directed mr-s mutants, Flag-W404A and Flag-G453A were generated and co-transfected with full-HA. |
| T31519 |
537-538 |
-LRB- |
denotes |
( |
| T31520 |
538-539 |
LS |
denotes |
C |
| T31522 |
539-540 |
-RRB- |
denotes |
) |
| T31523 |
541-545 |
NN |
denotes |
Flag |
| T31525 |
545-546 |
HYPH |
denotes |
- |
| T31524 |
546-552 |
VBN |
denotes |
tagged |
| T31527 |
553-556 |
CD |
denotes |
two |
| T31528 |
557-561 |
NN |
denotes |
site |
| T31530 |
561-562 |
HYPH |
denotes |
- |
| T31529 |
562-570 |
VBN |
denotes |
directed |
| T31531 |
571-573 |
NN |
denotes |
mr |
| T31533 |
573-574 |
HYPH |
denotes |
- |
| T31532 |
574-575 |
NN |
denotes |
s |
| T31526 |
576-583 |
NNS |
denotes |
mutants |
| T31534 |
583-585 |
, |
denotes |
, |
| T31535 |
585-589 |
NN |
denotes |
Flag |
| T31537 |
589-590 |
HYPH |
denotes |
- |
| T31536 |
590-595 |
NN |
denotes |
W404A |
| T31538 |
596-599 |
CC |
denotes |
and |
| T31539 |
600-604 |
NN |
denotes |
Flag |
| T31541 |
604-605 |
HYPH |
denotes |
- |
| T31540 |
605-610 |
NN |
denotes |
G453A |
| T31542 |
611-615 |
VBD |
denotes |
were |
| T31521 |
616-625 |
VBN |
denotes |
generated |
| T31543 |
626-629 |
CC |
denotes |
and |
| T31544 |
630-644 |
VBN |
denotes |
co-transfected |
| T31545 |
645-649 |
IN |
denotes |
with |
| T31546 |
650-654 |
JJ |
denotes |
full |
| T31548 |
654-655 |
HYPH |
denotes |
- |
| T31547 |
655-657 |
NN |
denotes |
HA |
| T31549 |
657-658 |
. |
denotes |
. |
| T31550 |
658-750 |
sentence |
denotes |
Each lane was co-immunoprecipitated by anti-HA antibody and detected by anti-Flag antibody. |
| T31551 |
659-663 |
DT |
denotes |
Each |
| T31552 |
664-668 |
NN |
denotes |
lane |
| T31554 |
669-672 |
VBD |
denotes |
was |
| T31553 |
673-694 |
VBN |
denotes |
co-immunoprecipitated |
| T31555 |
695-697 |
IN |
denotes |
by |
| T31556 |
698-705 |
JJ |
denotes |
anti-HA |
| T31557 |
706-714 |
NN |
denotes |
antibody |
| T31558 |
715-718 |
CC |
denotes |
and |
| T31559 |
719-727 |
VBN |
denotes |
detected |
| T31560 |
728-730 |
IN |
denotes |
by |
| T31561 |
731-740 |
JJ |
denotes |
anti-Flag |
| T31562 |
741-749 |
NN |
denotes |
antibody |
| T31563 |
749-750 |
. |
denotes |
. |
| R8967 |
T31407 |
T31408 |
det |
The,protein |
| R8968 |
T31408 |
T31412 |
nsubj |
protein,associate |
| R8969 |
T31409 |
T31410 |
compound |
mr,s |
| R8970 |
T31410 |
T31408 |
compound |
s,protein |
| R8971 |
T31411 |
T31410 |
punct |
-,s |
| R8972 |
T31413 |
T31412 |
aux |
can,associate |
| R8973 |
T31414 |
T31412 |
dep |
self,associate |
| R8974 |
T31415 |
T31412 |
punct |
-,associate |
| R8975 |
T31416 |
T31412 |
prep |
in,associate |
| R8976 |
T31417 |
T31418 |
amod |
mammalian,cells |
| R8977 |
T31418 |
T31416 |
pobj |
cells,in |
| R8978 |
T31419 |
T31412 |
punct |
.,associate |
| R8979 |
T31421 |
T31422 |
punct |
(,A |
| R8980 |
T31422 |
T31423 |
meta |
A,drawing |
| R8981 |
T31424 |
T31422 |
punct |
),A |
| R8982 |
T31425 |
T31423 |
amod |
Schematic,drawing |
| R8983 |
T31426 |
T31423 |
prep |
of,drawing |
| R8984 |
T31427 |
T31428 |
det |
the,constructs |
| R8985 |
T31428 |
T31426 |
pobj |
constructs,of |
| R8986 |
T31429 |
T31428 |
acl |
used,constructs |
| R8987 |
T31430 |
T31429 |
prep |
for,used |
| R8988 |
T31431 |
T31432 |
compound |
immunoprecipitation,assay |
| R8989 |
T31432 |
T31430 |
pobj |
assay,for |
| R8990 |
T31433 |
T31423 |
punct |
.,drawing |
| R8991 |
T31435 |
T31436 |
npadvmod |
HA,tagged |
| R8992 |
T31436 |
T31438 |
amod |
tagged,regions |
| R8993 |
T31437 |
T31436 |
punct |
-,tagged |
| R8994 |
T31438 |
T31471 |
nsubjpass |
regions,inserted |
| R8995 |
T31439 |
T31436 |
cc |
or,tagged |
| R8996 |
T31440 |
T31441 |
npadvmod |
Flag,tagged |
| R8997 |
T31441 |
T31436 |
conj |
tagged,tagged |
| R8998 |
T31442 |
T31441 |
punct |
-,tagged |
| R8999 |
T31443 |
T31444 |
amod |
full,length |
| R9000 |
T31444 |
T31438 |
nmod |
length,regions |
| R9001 |
T31445 |
T31444 |
punct |
-,length |
| R9002 |
T31446 |
T31447 |
punct |
(,1 |
| R9003 |
T31447 |
T31444 |
parataxis |
1,length |
| R9004 |
T31448 |
T31447 |
nmod |
amino,1 |
| R9005 |
T31449 |
T31447 |
nmod |
acids,1 |
| R9006 |
T31450 |
T31447 |
prep |
to,1 |
| R9007 |
T31451 |
T31450 |
pobj |
542,to |
| R9008 |
T31452 |
T31447 |
punct |
),1 |
| R9009 |
T31453 |
T31438 |
punct |
", ",regions |
| R9010 |
T31454 |
T31438 |
nmod |
ΔSAM,regions |
| R9011 |
T31455 |
T31456 |
punct |
(,1 |
| R9012 |
T31456 |
T31454 |
parataxis |
1,ΔSAM |
| R9013 |
T31457 |
T31456 |
nmod |
amino,1 |
| R9014 |
T31458 |
T31456 |
nmod |
acids,1 |
| R9015 |
T31459 |
T31456 |
prep |
to,1 |
| R9016 |
T31460 |
T31459 |
pobj |
400,to |
| R9017 |
T31461 |
T31456 |
punct |
),1 |
| R9018 |
T31462 |
T31454 |
cc |
and,ΔSAM |
| R9019 |
T31463 |
T31454 |
conj |
SAM,ΔSAM |
| R9020 |
T31464 |
T31465 |
punct |
(,401 |
| R9021 |
T31465 |
T31463 |
parataxis |
401,SAM |
| R9022 |
T31466 |
T31465 |
nmod |
amino,401 |
| R9023 |
T31467 |
T31465 |
nmod |
acids,401 |
| R9024 |
T31468 |
T31465 |
prep |
to,401 |
| R9025 |
T31469 |
T31468 |
pobj |
542,to |
| R9026 |
T31470 |
T31465 |
punct |
),401 |
| R9027 |
T31472 |
T31471 |
auxpass |
were,inserted |
| R9028 |
T31473 |
T31471 |
prep |
into,inserted |
| R9029 |
T31474 |
T31475 |
compound |
pcDNA3,vector |
| R9030 |
T31475 |
T31473 |
pobj |
vector,into |
| R9031 |
T31476 |
T31471 |
punct |
", ",inserted |
| R9032 |
T31477 |
T31471 |
advmod |
respectively,inserted |
| R9033 |
T31478 |
T31471 |
punct |
.,inserted |
| R9034 |
T31480 |
T31481 |
punct |
(,B |
| R9035 |
T31481 |
T31482 |
meta |
B,transfected |
| R9036 |
T31483 |
T31481 |
punct |
),B |
| R9037 |
T31484 |
T31485 |
det |
The,constructs |
| R9038 |
T31485 |
T31482 |
nsubjpass |
constructs,transfected |
| R9039 |
T31486 |
T31485 |
acl |
indicated,constructs |
| R9040 |
T31487 |
T31486 |
advmod |
above,indicated |
| R9041 |
T31488 |
T31482 |
auxpass |
were,transfected |
| R9042 |
T31489 |
T31482 |
prep |
into,transfected |
| R9043 |
T31490 |
T31491 |
compound |
HEK293T,cells |
| R9044 |
T31491 |
T31489 |
pobj |
cells,into |
| R9045 |
T31492 |
T31482 |
punct |
.,transfected |
| R9046 |
T31494 |
T31495 |
det |
Each,lane |
| R9047 |
T31495 |
T31496 |
nsubjpass |
lane,co-immunoprecipitated |
| R9048 |
T31497 |
T31496 |
auxpass |
was,co-immunoprecipitated |
| R9049 |
T31498 |
T31496 |
agent |
by,co-immunoprecipitated |
| R9050 |
T31499 |
T31500 |
amod |
anti-Flag,antibody |
| R9051 |
T31500 |
T31498 |
pobj |
antibody,by |
| R9052 |
T31501 |
T31496 |
cc |
and,co-immunoprecipitated |
| R9053 |
T31502 |
T31496 |
conj |
detected,co-immunoprecipitated |
| R9054 |
T31503 |
T31502 |
agent |
by,detected |
| R9055 |
T31504 |
T31505 |
amod |
anti-HA,antibody |
| R9056 |
T31505 |
T31503 |
pobj |
antibody,by |
| R9057 |
T31506 |
T31496 |
punct |
.,co-immunoprecipitated |
| R9058 |
T31508 |
T31509 |
compound |
Input,lysates |
| R9059 |
T31509 |
T31511 |
nsubjpass |
lysates,shown |
| R9060 |
T31510 |
T31509 |
compound |
protein,lysates |
| R9061 |
T31512 |
T31511 |
auxpass |
are,shown |
| R9062 |
T31513 |
T31511 |
prep |
in,shown |
| R9063 |
T31514 |
T31515 |
det |
the,panels |
| R9064 |
T31515 |
T31513 |
pobj |
panels,in |
| R9065 |
T31516 |
T31515 |
amod |
lower,panels |
| R9066 |
T31517 |
T31511 |
punct |
.,shown |
| R9067 |
T31519 |
T31520 |
punct |
(,C |
| R9068 |
T31520 |
T31521 |
meta |
C,generated |
| R9069 |
T31522 |
T31520 |
punct |
),C |
| R9070 |
T31523 |
T31524 |
npadvmod |
Flag,tagged |
| R9071 |
T31524 |
T31526 |
amod |
tagged,mutants |
| R9072 |
T31525 |
T31524 |
punct |
-,tagged |
| R9073 |
T31526 |
T31521 |
nsubjpass |
mutants,generated |
| R9074 |
T31527 |
T31528 |
nummod |
two,site |
| R9075 |
T31528 |
T31529 |
npadvmod |
site,directed |
| R9076 |
T31529 |
T31526 |
amod |
directed,mutants |
| R9077 |
T31530 |
T31529 |
punct |
-,directed |
| R9078 |
T31531 |
T31532 |
compound |
mr,s |
| R9079 |
T31532 |
T31526 |
compound |
s,mutants |
| R9080 |
T31533 |
T31532 |
punct |
-,s |
| R9081 |
T31534 |
T31526 |
punct |
", ",mutants |
| R9082 |
T31535 |
T31536 |
compound |
Flag,W404A |
| R9083 |
T31536 |
T31526 |
appos |
W404A,mutants |
| R9084 |
T31537 |
T31536 |
punct |
-,W404A |
| R9085 |
T31538 |
T31536 |
cc |
and,W404A |
| R9086 |
T31539 |
T31540 |
compound |
Flag,G453A |
| R9087 |
T31540 |
T31536 |
conj |
G453A,W404A |
| R9088 |
T31541 |
T31540 |
punct |
-,G453A |
| R9089 |
T31542 |
T31521 |
auxpass |
were,generated |
| R9090 |
T31543 |
T31521 |
cc |
and,generated |
| R9091 |
T31544 |
T31521 |
conj |
co-transfected,generated |
| R9092 |
T31545 |
T31544 |
prep |
with,co-transfected |
| R9093 |
T31546 |
T31547 |
amod |
full,HA |
| R9094 |
T31547 |
T31545 |
pobj |
HA,with |
| R9095 |
T31548 |
T31547 |
punct |
-,HA |
| R9096 |
T31549 |
T31521 |
punct |
.,generated |
| R9097 |
T31551 |
T31552 |
det |
Each,lane |
| R9098 |
T31552 |
T31553 |
nsubjpass |
lane,co-immunoprecipitated |
| R9099 |
T31554 |
T31553 |
auxpass |
was,co-immunoprecipitated |
| R9100 |
T31555 |
T31553 |
agent |
by,co-immunoprecipitated |
| R9101 |
T31556 |
T31557 |
amod |
anti-HA,antibody |
| R9102 |
T31557 |
T31555 |
pobj |
antibody,by |
| R9103 |
T31558 |
T31553 |
cc |
and,co-immunoprecipitated |
| R9104 |
T31559 |
T31553 |
conj |
detected,co-immunoprecipitated |
| R9105 |
T31560 |
T31559 |
agent |
by,detected |
| R9106 |
T31561 |
T31562 |
amod |
anti-Flag,antibody |
| R9107 |
T31562 |
T31560 |
pobj |
antibody,by |
| R9108 |
T31563 |
T31553 |
punct |
.,co-immunoprecipitated |
