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PMC:1382200 / 1818-4790 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T3283 2-4 CD denotes 13
T3284 5-10 NNS denotes genes
T3285 11-13 IN denotes in
T3286 14-21 NNS denotes mammals
T3287 22-23 -LRB- denotes [
T3288 23-24 CD denotes 1
T3289 24-25 SYM denotes -
T3290 25-26 CD denotes 3
T3291 26-27 -RRB- denotes ]
T3292 27-28 . denotes .
T3293 28-261 sentence denotes Both are heterodimeric enzymes consisting of distinct 80 kDa catalytic subunits, encoded by the Capn1 (μ-80 k) and Capn2 (m-80 k) genes, respectively, that associate with a common 28 kDa regulatory subunit encoded by the Capn4 gene.
T3294 29-33 DT denotes Both
T3295 34-37 VBP denotes are
T3296 38-51 JJ denotes heterodimeric
T3297 52-59 NNS denotes enzymes
T3298 60-70 VBG denotes consisting
T3299 71-73 IN denotes of
T3300 74-82 JJ denotes distinct
T3302 83-85 CD denotes 80
T3303 86-89 NN denotes kDa
T3304 90-99 JJ denotes catalytic
T3301 100-108 NNS denotes subunits
T3305 108-110 , denotes ,
T3306 110-117 VBN denotes encoded
T3307 118-120 IN denotes by
T3308 121-124 DT denotes the
T3310 125-130 NN denotes Capn1
T3311 131-132 -LRB- denotes (
T3313 132-133 NN denotes μ
T3314 133-134 HYPH denotes -
T3315 134-136 CD denotes 80
T3312 137-138 NN denotes k
T3316 138-139 -RRB- denotes )
T3317 140-143 CC denotes and
T3318 144-149 NN denotes Capn2
T3319 150-151 -LRB- denotes (
T3321 151-152 NN denotes m
T3322 152-153 HYPH denotes -
T3323 153-155 CD denotes 80
T3320 156-157 NN denotes k
T3324 157-158 -RRB- denotes )
T3309 159-164 NNS denotes genes
T3325 164-166 , denotes ,
T3326 166-178 RB denotes respectively
T3327 178-180 , denotes ,
T3328 180-184 WDT denotes that
T3329 185-194 VBP denotes associate
T3330 195-199 IN denotes with
T3331 200-201 DT denotes a
T3333 202-208 JJ denotes common
T3334 209-211 CD denotes 28
T3335 212-215 NN denotes kDa
T3336 216-226 JJ denotes regulatory
T3332 227-234 NN denotes subunit
T3337 235-242 VBN denotes encoded
T3338 243-245 IN denotes by
T3339 246-249 DT denotes the
T3341 250-255 NN denotes Capn4
T3340 256-260 NN denotes gene
T3342 260-261 . denotes .
T3343 261-431 sentence denotes The μ-80 k and m-80 k subunits share 62% amino acid sequence identity, and are very similar in terms of structure, protein chemistry, and in vitro substrate specificity.
T3344 262-265 DT denotes The
T3346 266-267 NN denotes μ
T3348 267-268 HYPH denotes -
T3349 268-270 CD denotes 80
T3347 271-272 NN denotes k
T3350 273-276 CC denotes and
T3351 277-278 NN denotes m
T3353 278-279 HYPH denotes -
T3354 279-281 CD denotes 80
T3352 282-283 NN denotes k
T3345 284-292 NNS denotes subunits
T3355 293-298 VBP denotes share
T3356 299-301 CD denotes 62
T3357 301-302 NN denotes %
T3359 303-308 NN denotes amino
T3360 309-313 NN denotes acid
T3361 314-322 NN denotes sequence
T3358 323-331 NN denotes identity
T3362 331-333 , denotes ,
T3363 333-336 CC denotes and
T3364 337-340 VBP denotes are
T3365 341-345 RB denotes very
T3366 346-353 JJ denotes similar
T3367 354-356 IN denotes in
T3368 357-362 NNS denotes terms
T3369 363-365 IN denotes of
T3370 366-375 NN denotes structure
T3371 375-377 , denotes ,
T3372 377-384 NN denotes protein
T3373 385-394 NN denotes chemistry
T3374 394-396 , denotes ,
T3375 396-399 CC denotes and
T3376 400-402 FW denotes in
T3377 403-408 FW denotes vitro
T3379 409-418 NN denotes substrate
T3378 419-430 NN denotes specificity
T3380 430-431 . denotes .
T3381 431-601 sentence denotes Despite these similarities, the differential expression patterns of μ- and m-calpain in mammalian tissues suggest they have some isoform specific and distinct functions.
T3382 432-439 IN denotes Despite
T3384 440-445 DT denotes these
T3385 446-458 NNS denotes similarities
T3386 458-460 , denotes ,
T3387 460-463 DT denotes the
T3389 464-476 JJ denotes differential
T3390 477-487 NN denotes expression
T3388 488-496 NNS denotes patterns
T3391 497-499 IN denotes of
T3392 500-501 NN denotes μ
T3394 501-502 HYPH denotes -
T3395 503-506 CC denotes and
T3396 507-508 NN denotes m
T3397 508-509 HYPH denotes -
T3393 509-516 NN denotes calpain
T3398 517-519 IN denotes in
T3399 520-529 JJ denotes mammalian
T3400 530-537 NNS denotes tissues
T3383 538-545 VBP denotes suggest
T3401 546-550 PRP denotes they
T3402 551-555 VBP denotes have
T3403 556-560 DT denotes some
T3405 561-568 NN denotes isoform
T3406 569-577 JJ denotes specific
T3407 578-581 CC denotes and
T3408 582-590 JJ denotes distinct
T3404 591-600 NNS denotes functions
T3409 600-601 . denotes .
T3410 601-763 sentence denotes The μ and m designations derive from the levels of Ca2+ required in vitro for optimal activation; 10–50 μM Ca2+ for μ-calpain and 0.3–0.35 mM Ca2+ for m-calpain.
T3411 602-605 DT denotes The
T3413 606-607 NN denotes μ
T3414 608-611 CC denotes and
T3415 612-613 NN denotes m
T3412 614-626 NNS denotes designations
T3416 627-633 VBP denotes derive
T3417 634-638 IN denotes from
T3418 639-642 DT denotes the
T3419 643-649 NNS denotes levels
T3420 650-652 IN denotes of
T3421 653-656 NN denotes Ca2
T3422 656-657 SYM denotes +
T3423 658-666 VBN denotes required
T3424 667-669 FW denotes in
T3425 670-675 FW denotes vitro
T3426 676-679 IN denotes for
T3427 680-687 JJ denotes optimal
T3428 688-698 NN denotes activation
T3429 698-699 : denotes ;
T3430 700-702 CD denotes 10
T3432 702-703 SYM denotes –
T3431 703-705 CD denotes 50
T3433 706-708 NN denotes μM
T3434 709-712 NN denotes Ca2
T3435 712-713 SYM denotes +
T3436 714-717 IN denotes for
T3437 718-719 NN denotes μ
T3439 719-720 HYPH denotes -
T3438 720-727 NN denotes calpain
T3440 728-731 CC denotes and
T3441 732-735 CD denotes 0.3
T3443 735-736 SYM denotes –
T3442 736-740 CD denotes 0.35
T3444 741-743 NN denotes mM
T3445 744-747 NN denotes Ca2
T3446 747-748 SYM denotes +
T3447 749-752 IN denotes for
T3448 753-754 NN denotes m
T3450 754-755 HYPH denotes -
T3449 755-762 NN denotes calpain
T3451 762-763 . denotes .
T3452 763-925 sentence denotes It is generally assumed that μ- and m-calpain maintain their differential sensitivities to calcium in vivo, although this has not yet been strictly demonstrated.
T3453 764-766 PRP denotes It
T3455 767-769 VBZ denotes is
T3456 770-779 RB denotes generally
T3454 780-787 VBN denotes assumed
T3457 788-792 IN denotes that
T3459 793-794 NN denotes μ
T3461 794-795 HYPH denotes -
T3462 796-799 CC denotes and
T3463 800-801 NN denotes m
T3464 801-802 HYPH denotes -
T3460 802-809 NN denotes calpain
T3458 810-818 VBP denotes maintain
T3465 819-824 PRP$ denotes their
T3467 825-837 JJ denotes differential
T3466 838-851 NNS denotes sensitivities
T3468 852-854 IN denotes to
T3469 855-862 NN denotes calcium
T3470 863-865 FW denotes in
T3471 866-870 FW denotes vivo
T3472 870-872 , denotes ,
T3473 872-880 IN denotes although
T3475 881-885 DT denotes this
T3476 886-889 VBZ denotes has
T3477 890-893 RB denotes not
T3478 894-897 RB denotes yet
T3479 898-902 VBN denotes been
T3480 903-911 RB denotes strictly
T3474 912-924 VBN denotes demonstrated
T3481 924-925 . denotes .
T3482 925-1111 sentence denotes Furthermore, since the cytoplasmic free Ca2+ concentration is typically less than 1 μM, it is also assumed that other in vivo factors must contribute to regulation of these enzymes [3].
T3483 926-937 RB denotes Furthermore
T3485 937-939 , denotes ,
T3486 939-944 IN denotes since
T3488 945-948 DT denotes the
T3490 949-960 JJ denotes cytoplasmic
T3491 961-965 JJ denotes free
T3492 966-969 NN denotes Ca2
T3493 969-970 SYM denotes +
T3489 971-984 NN denotes concentration
T3487 985-987 VBZ denotes is
T3494 988-997 RB denotes typically
T3495 998-1002 JJR denotes less
T3497 1003-1007 IN denotes than
T3496 1008-1009 CD denotes 1
T3498 1010-1012 NN denotes μM
T3499 1012-1014 , denotes ,
T3500 1014-1016 PRP denotes it
T3501 1017-1019 VBZ denotes is
T3502 1020-1024 RB denotes also
T3484 1025-1032 VBN denotes assumed
T3503 1033-1037 IN denotes that
T3505 1038-1043 JJ denotes other
T3507 1044-1046 FW denotes in
T3508 1047-1051 FW denotes vivo
T3506 1052-1059 NNS denotes factors
T3509 1060-1064 MD denotes must
T3504 1065-1075 VB denotes contribute
T3510 1076-1078 IN denotes to
T3511 1079-1089 NN denotes regulation
T3512 1090-1092 IN denotes of
T3513 1093-1098 DT denotes these
T3514 1099-1106 NNS denotes enzymes
T3515 1107-1108 -LRB- denotes [
T3516 1108-1109 CD denotes 3
T3517 1109-1110 -RRB- denotes ]
T3518 1110-1111 . denotes .
T3519 1111-1281 sentence denotes Without apriori knowledge of the factors regulating calpain activity or their relevant substrates, elucidation of biological functions for calpains presents a challenge.
T3520 1112-1119 IN denotes Without
T3522 1120-1127 FW denotes apriori
T3523 1128-1137 NN denotes knowledge
T3524 1138-1140 IN denotes of
T3525 1141-1144 DT denotes the
T3526 1145-1152 NNS denotes factors
T3527 1153-1163 VBG denotes regulating
T3528 1164-1171 NN denotes calpain
T3529 1172-1180 NN denotes activity
T3530 1181-1183 CC denotes or
T3531 1184-1189 PRP$ denotes their
T3533 1190-1198 JJ denotes relevant
T3532 1199-1209 NNS denotes substrates
T3534 1209-1211 , denotes ,
T3535 1211-1222 NN denotes elucidation
T3536 1223-1225 IN denotes of
T3537 1226-1236 JJ denotes biological
T3538 1237-1246 NNS denotes functions
T3539 1247-1250 IN denotes for
T3540 1251-1259 NNS denotes calpains
T3521 1260-1268 VBZ denotes presents
T3541 1269-1270 DT denotes a
T3542 1271-1280 NN denotes challenge
T3543 1280-1281 . denotes .
T3544 1281-1696 sentence denotes Research on calpains has linked them with a wide variety of functions including muscle growth, development, degeneration (3), neuronal growth and neurodegeneration [4], cell cycle progression [5,6], signal cascades triggered by integrins and growth factors [7], membrane protrusion [8], remodeling of the cytoskeleton and cell migration [9-15], and regulation of cell death via both necrosis and apoptosis [16-22].
T3545 1282-1290 NN denotes Research
T3547 1291-1293 IN denotes on
T3548 1294-1302 NNS denotes calpains
T3549 1303-1306 VBZ denotes has
T3546 1307-1313 VBN denotes linked
T3550 1314-1318 PRP denotes them
T3551 1319-1323 IN denotes with
T3552 1324-1325 DT denotes a
T3554 1326-1330 JJ denotes wide
T3553 1331-1338 NN denotes variety
T3555 1339-1341 IN denotes of
T3556 1342-1351 NNS denotes functions
T3557 1352-1361 VBG denotes including
T3558 1362-1368 NN denotes muscle
T3559 1369-1375 NN denotes growth
T3560 1375-1377 , denotes ,
T3561 1377-1388 NN denotes development
T3562 1388-1390 , denotes ,
T3563 1390-1402 NN denotes degeneration
T3564 1403-1404 -LRB- denotes (
T3565 1404-1405 CD denotes 3
T3566 1405-1406 -RRB- denotes )
T3567 1406-1408 , denotes ,
T3568 1408-1416 JJ denotes neuronal
T3569 1417-1423 NN denotes growth
T3570 1424-1427 CC denotes and
T3571 1428-1445 NN denotes neurodegeneration
T3572 1446-1447 -LRB- denotes [
T3573 1447-1448 CD denotes 4
T3574 1448-1449 -RRB- denotes ]
T3575 1449-1451 , denotes ,
T3576 1451-1455 NN denotes cell
T3577 1456-1461 NN denotes cycle
T3578 1462-1473 NN denotes progression
T3579 1474-1475 -LRB- denotes [
T3581 1475-1476 CD denotes 5
T3582 1476-1477 , denotes ,
T3580 1477-1478 CD denotes 6
T3583 1478-1479 -RRB- denotes ]
T3584 1479-1481 , denotes ,
T3585 1481-1487 NN denotes signal
T3586 1488-1496 NNS denotes cascades
T3587 1497-1506 VBN denotes triggered
T3588 1507-1509 IN denotes by
T3589 1510-1519 NNS denotes integrins
T3590 1520-1523 CC denotes and
T3591 1524-1530 NN denotes growth
T3592 1531-1538 NNS denotes factors
T3593 1539-1540 -LRB- denotes [
T3594 1540-1541 CD denotes 7
T3595 1541-1542 -RRB- denotes ]
T3596 1542-1544 , denotes ,
T3597 1544-1552 NN denotes membrane
T3598 1553-1563 NN denotes protrusion
T3599 1564-1565 -LRB- denotes [
T3600 1565-1566 CD denotes 8
T3601 1566-1567 -RRB- denotes ]
T3602 1567-1569 , denotes ,
T3603 1569-1579 VBG denotes remodeling
T3604 1580-1582 IN denotes of
T3605 1583-1586 DT denotes the
T3606 1587-1599 NN denotes cytoskeleton
T3607 1600-1603 CC denotes and
T3608 1604-1608 NN denotes cell
T3609 1609-1618 NN denotes migration
T3610 1619-1620 -LRB- denotes [
T3611 1620-1621 CD denotes 9
T3612 1621-1622 SYM denotes -
T3613 1622-1624 CD denotes 15
T3614 1624-1625 -RRB- denotes ]
T3615 1625-1627 , denotes ,
T3616 1627-1630 CC denotes and
T3617 1631-1641 NN denotes regulation
T3618 1642-1644 IN denotes of
T3619 1645-1649 NN denotes cell
T3620 1650-1655 NN denotes death
T3621 1656-1659 IN denotes via
T3622 1660-1664 CC denotes both
T3623 1665-1673 NN denotes necrosis
T3624 1674-1677 CC denotes and
T3625 1678-1687 NN denotes apoptosis
T3626 1688-1689 -LRB- denotes [
T3627 1689-1691 CD denotes 16
T3628 1691-1692 SYM denotes -
T3629 1692-1694 CD denotes 22
T3630 1694-1695 -RRB- denotes ]
T3631 1695-1696 . denotes .
T3632 1696-1848 sentence denotes To date, the literature suggests a complex interplay between caspases and calpains [23,24] and impact of calpain on cell death pathway components [25].
T3633 1697-1699 IN denotes To
T3635 1700-1704 NN denotes date
T3636 1704-1706 , denotes ,
T3637 1706-1709 DT denotes the
T3638 1710-1720 NN denotes literature
T3634 1721-1729 VBZ denotes suggests
T3639 1730-1731 DT denotes a
T3641 1732-1739 JJ denotes complex
T3640 1740-1749 NN denotes interplay
T3642 1750-1757 IN denotes between
T3643 1758-1766 NNS denotes caspases
T3644 1767-1770 CC denotes and
T3645 1771-1779 NNS denotes calpains
T3646 1780-1781 -LRB- denotes [
T3648 1781-1783 CD denotes 23
T3649 1783-1784 , denotes ,
T3647 1784-1786 CD denotes 24
T3650 1786-1787 -RRB- denotes ]
T3651 1788-1791 CC denotes and
T3652 1792-1798 NN denotes impact
T3653 1799-1801 IN denotes of
T3654 1802-1809 NN denotes calpain
T3655 1810-1812 IN denotes on
T3656 1813-1817 NN denotes cell
T3657 1818-1823 NN denotes death
T3659 1824-1831 NN denotes pathway
T3658 1832-1842 NNS denotes components
T3660 1843-1844 -LRB- denotes [
T3661 1844-1846 CD denotes 25
T3662 1846-1847 -RRB- denotes ]
T3663 1847-1848 . denotes .
T3664 1848-2011 sentence denotes The lack of highly specific cell-permeable inhibitors of calpains contributes to the challenge of investigating and defining calpain functions in these processes.
T3665 1849-1852 DT denotes The
T3666 1853-1857 NN denotes lack
T3668 1858-1860 IN denotes of
T3669 1861-1867 RB denotes highly
T3670 1868-1876 JJ denotes specific
T3672 1877-1881 NN denotes cell
T3674 1881-1882 HYPH denotes -
T3673 1882-1891 JJ denotes permeable
T3671 1892-1902 NNS denotes inhibitors
T3675 1903-1905 IN denotes of
T3676 1906-1914 NNS denotes calpains
T3667 1915-1926 VBZ denotes contributes
T3677 1927-1929 IN denotes to
T3678 1930-1933 DT denotes the
T3679 1934-1943 NN denotes challenge
T3680 1944-1946 IN denotes of
T3681 1947-1960 VBG denotes investigating
T3682 1961-1964 CC denotes and
T3683 1965-1973 VBG denotes defining
T3684 1974-1981 NN denotes calpain
T3685 1982-1991 NNS denotes functions
T3686 1992-1994 IN denotes in
T3687 1995-2000 DT denotes these
T3688 2001-2010 NNS denotes processes
T3689 2010-2011 . denotes .
T3690 2011-2218 sentence denotes Although over-expression of calpastatin, the endogenous protein inhibitor of μ- and m-calpain provides an important approach for these efforts, it will not distinguish isoform specific functions [24,26,27].
T3691 2012-2020 IN denotes Although
T3693 2021-2036 NN denotes over-expression
T3694 2037-2039 IN denotes of
T3695 2040-2051 NN denotes calpastatin
T3696 2051-2053 , denotes ,
T3697 2053-2056 DT denotes the
T3699 2057-2067 JJ denotes endogenous
T3700 2068-2075 NN denotes protein
T3698 2076-2085 NN denotes inhibitor
T3701 2086-2088 IN denotes of
T3702 2089-2090 NN denotes μ
T3704 2090-2091 HYPH denotes -
T3705 2092-2095 CC denotes and
T3706 2096-2097 NN denotes m
T3707 2097-2098 HYPH denotes -
T3703 2098-2105 NN denotes calpain
T3692 2106-2114 VBZ denotes provides
T3709 2115-2117 DT denotes an
T3711 2118-2127 JJ denotes important
T3710 2128-2136 NN denotes approach
T3712 2137-2140 IN denotes for
T3713 2141-2146 DT denotes these
T3714 2147-2154 NNS denotes efforts
T3715 2154-2156 , denotes ,
T3716 2156-2158 PRP denotes it
T3717 2159-2163 MD denotes will
T3718 2164-2167 RB denotes not
T3708 2168-2179 VB denotes distinguish
T3719 2180-2187 NN denotes isoform
T3720 2188-2196 JJ denotes specific
T3721 2197-2206 NNS denotes functions
T3722 2207-2208 -LRB- denotes [
T3724 2208-2210 CD denotes 24
T3725 2210-2211 , denotes ,
T3726 2211-2213 CD denotes 26
T3727 2213-2214 , denotes ,
T3723 2214-2216 CD denotes 27
T3728 2216-2217 -RRB- denotes ]
T3729 2217-2218 . denotes .
T3730 2218-2458 sentence denotes Some work has suggested isoform specific roles, such as a role for m-calpain in epidermal growth factor (EGF)-induced cell motility [28,29] and a role for μ-calpain in interferon-inducible protein 9-induced migration of keratinocytes [28].
T3731 2219-2223 DT denotes Some
T3732 2224-2228 NN denotes work
T3734 2229-2232 VBZ denotes has
T3733 2233-2242 VBN denotes suggested
T3735 2243-2250 NN denotes isoform
T3736 2251-2259 JJ denotes specific
T3737 2260-2265 NNS denotes roles
T3738 2265-2267 , denotes ,
T3739 2267-2271 JJ denotes such
T3740 2272-2274 IN denotes as
T3741 2275-2276 DT denotes a
T3742 2277-2281 NN denotes role
T3743 2282-2285 IN denotes for
T3744 2286-2287 NN denotes m
T3746 2287-2288 HYPH denotes -
T3745 2288-2295 NN denotes calpain
T3747 2296-2298 IN denotes in
T3748 2299-2308 JJ denotes epidermal
T3750 2309-2315 NN denotes growth
T3749 2316-2322 NN denotes factor
T3752 2323-2324 -LRB- denotes (
T3753 2324-2327 NN denotes EGF
T3754 2327-2328 -RRB- denotes )
T3755 2328-2329 HYPH denotes -
T3751 2329-2336 VBN denotes induced
T3757 2337-2341 NN denotes cell
T3756 2342-2350 NN denotes motility
T3758 2351-2352 -LRB- denotes [
T3760 2352-2354 CD denotes 28
T3761 2354-2355 , denotes ,
T3759 2355-2357 CD denotes 29
T3762 2357-2358 -RRB- denotes ]
T3763 2359-2362 CC denotes and
T3764 2363-2364 DT denotes a
T3765 2365-2369 NN denotes role
T3766 2370-2373 IN denotes for
T3767 2374-2375 NN denotes μ
T3769 2375-2376 HYPH denotes -
T3768 2376-2383 NN denotes calpain
T3770 2384-2386 IN denotes in
T3771 2387-2397 NN denotes interferon
T3773 2397-2398 HYPH denotes -
T3772 2398-2407 JJ denotes inducible
T3775 2408-2415 NN denotes protein
T3777 2416-2417 CD denotes 9
T3778 2417-2418 HYPH denotes -
T3776 2418-2425 VBN denotes induced
T3774 2426-2435 NN denotes migration
T3779 2436-2438 IN denotes of
T3780 2439-2452 NNS denotes keratinocytes
T3781 2453-2454 -LRB- denotes [
T3782 2454-2456 CD denotes 28
T3783 2456-2457 -RRB- denotes ]
T3784 2457-2458 . denotes .
T3785 2458-2643 sentence denotes A cell permeable calpain inhibitor (which likely inhibits other thiol-proteases as well) has been used to select cells lacking μ-calpain which display reduced proliferation rates [30].
T3786 2459-2460 DT denotes A
T3788 2461-2465 NN denotes cell
T3789 2466-2475 JJ denotes permeable
T3790 2476-2483 NN denotes calpain
T3787 2484-2493 NN denotes inhibitor
T3792 2494-2495 -LRB- denotes (
T3793 2495-2500 WDT denotes which
T3795 2501-2507 RB denotes likely
T3794 2508-2516 VBZ denotes inhibits
T3796 2517-2522 JJ denotes other
T3798 2523-2528 NN denotes thiol
T3799 2528-2529 HYPH denotes -
T3797 2529-2538 NNS denotes proteases
T3800 2539-2541 RB denotes as
T3801 2542-2546 RB denotes well
T3802 2546-2547 -RRB- denotes )
T3803 2548-2551 VBZ denotes has
T3804 2552-2556 VBN denotes been
T3791 2557-2561 VBN denotes used
T3805 2562-2564 TO denotes to
T3806 2565-2571 VB denotes select
T3807 2572-2577 NNS denotes cells
T3808 2578-2585 VBG denotes lacking
T3809 2586-2587 NN denotes μ
T3811 2587-2588 HYPH denotes -
T3810 2588-2595 NN denotes calpain
T3812 2596-2601 WDT denotes which
T3813 2602-2609 VBP denotes display
T3814 2610-2617 VBN denotes reduced
T3816 2618-2631 NN denotes proliferation
T3815 2632-2637 NNS denotes rates
T3817 2638-2639 -LRB- denotes [
T3818 2639-2641 CD denotes 30
T3819 2641-2642 -RRB- denotes ]
T3820 2642-2643 . denotes .
T3821 2643-2776 sentence denotes Interestingly, m-calpain expression persisted in these cells, suggesting a possible requirement of m-calpain for cell survival [30].
T3822 2644-2657 RB denotes Interestingly
T3824 2657-2659 , denotes ,
T3825 2659-2660 NN denotes m
T3827 2660-2661 HYPH denotes -
T3826 2661-2668 NN denotes calpain
T3828 2669-2679 NN denotes expression
T3823 2680-2689 VBD denotes persisted
T3829 2690-2692 IN denotes in
T3830 2693-2698 DT denotes these
T3831 2699-2704 NNS denotes cells
T3832 2704-2706 , denotes ,
T3833 2706-2716 VBG denotes suggesting
T3834 2717-2718 DT denotes a
T3836 2719-2727 JJ denotes possible
T3835 2728-2739 NN denotes requirement
T3837 2740-2742 IN denotes of
T3838 2743-2744 NN denotes m
T3840 2744-2745 HYPH denotes -
T3839 2745-2752 NN denotes calpain
T3841 2753-2756 IN denotes for
T3842 2757-2761 NN denotes cell
T3843 2762-2770 NN denotes survival
T3844 2771-2772 -LRB- denotes [
T3845 2772-2774 CD denotes 30
T3846 2774-2775 -RRB- denotes ]
T3847 2775-2776 . denotes .
T3848 2776-2961 sentence denotes Targeted gene deletion in mice provides a powerful approach to determining the physiological roles of μ- and m-calpain and the opportunity to approach their isoform specific functions.
T3849 2777-2785 VBN denotes Targeted
T3851 2786-2790 NN denotes gene
T3850 2791-2799 NN denotes deletion
T3853 2800-2802 IN denotes in
T3854 2803-2807 NNS denotes mice
T3852 2808-2816 VBZ denotes provides
T3855 2817-2818 DT denotes a
T3857 2819-2827 JJ denotes powerful
T3856 2828-2836 NN denotes approach
T3858 2837-2839 IN denotes to
T3859 2840-2851 VBG denotes determining
T3860 2852-2855 DT denotes the
T3862 2856-2869 JJ denotes physiological
T3861 2870-2875 NNS denotes roles
T3863 2876-2878 IN denotes of
T3864 2879-2880 NN denotes μ
T3866 2880-2881 HYPH denotes -
T3867 2882-2885 CC denotes and
T3868 2886-2887 NN denotes m
T3869 2887-2888 HYPH denotes -
T3865 2888-2895 NN denotes calpain
T3870 2896-2899 CC denotes and
T3871 2900-2903 DT denotes the
T3872 2904-2915 NN denotes opportunity
T3873 2916-2918 TO denotes to
T3874 2919-2927 VB denotes approach
T3875 2928-2933 PRP$ denotes their
T3877 2934-2941 NN denotes isoform
T3878 2942-2950 JJ denotes specific
T3876 2951-2960 NNS denotes functions
T3879 2960-2961 . denotes .
T3881 2962-2969 JJ denotes Initial
R282 T3323 T3320 nummod 80,k
R287 T3324 T3320 punct ),k
R288 T3325 T3306 punct ", ",encoded
R289 T3326 T3306 advmod respectively,encoded
R291 T3327 T3301 punct ", ",subunits
R294 T3328 T3329 dep that,associate
R297 T3329 T3301 relcl associate,subunits
R301 T3330 T3329 prep with,associate
R305 T3331 T3332 det a,subunit
R309 T3332 T3330 pobj subunit,with
R313 T3333 T3332 amod common,subunit
R317 T3334 T3335 nummod 28,kDa
R319 T3335 T3332 nmod kDa,subunit
R333 T3336 T3332 amod regulatory,subunit
R338 T3337 T3332 acl encoded,subunit
R339 T3283 T3284 nummod 13,genes
R342 T3338 T3337 agent by,encoded
R343 T3286 T3285 pobj mammals,in
R344 T3287 T3288 punct [,1
R346 T3339 T3340 det the,gene
R347 T3289 T3290 punct -,3
R348 T3290 T3288 prep 3,1
R349 T3291 T3288 punct ],1
R350 T3340 T3338 pobj gene,by
R352 T3341 T3340 compound Capn4,gene
R353 T3294 T3295 nsubj Both,are
R354 T3296 T3297 amod heterodimeric,enzymes
R355 T3342 T3295 punct .,are
R356 T3297 T3295 attr enzymes,are
R357 T3298 T3297 acl consisting,enzymes
R358 T3299 T3298 prep of,consisting
R359 T3344 T3345 det The,subunits
R360 T3300 T3301 amod distinct,subunits
R361 T3301 T3299 pobj subunits,of
R362 T3302 T3303 nummod 80,kDa
R363 T3303 T3301 nmod kDa,subunits
R364 T3345 T3355 nsubj subunits,share
R365 T3304 T3301 amod catalytic,subunits
R366 T3305 T3301 punct ", ",subunits
R367 T3346 T3347 nmod μ,k
R368 T3347 T3345 nmod k,subunits
R369 T3306 T3301 acl encoded,subunits
R370 T3307 T3306 agent by,encoded
R371 T3348 T3346 punct -,μ
R372 T3308 T3309 det the,genes
R373 T3309 T3307 pobj genes,by
R374 T3310 T3309 nmod Capn1,genes
R375 T3311 T3312 punct (,k
R376 T3349 T3346 nummod 80,μ
R377 T3312 T3310 parataxis k,Capn1
R378 T3313 T3312 nmod μ,k
R379 T3314 T3312 punct -,k
R380 T3315 T3312 nummod 80,k
R381 T3350 T3347 cc and,k
R382 T3316 T3312 punct ),k
R383 T3317 T3310 cc and,Capn1
R384 T3318 T3310 conj Capn2,Capn1
R385 T3351 T3352 nmod m,k
R386 T3319 T3320 punct (,k
R387 T3320 T3318 parataxis k,Capn2
R388 T3321 T3320 nmod m,k
R389 T3352 T3347 conj k,k
R390 T3322 T3320 punct -,k
R391 T3353 T3351 punct -,m
R392 T3354 T3351 nummod 80,m
R393 T3356 T3357 nummod 62,%
R394 T3429 T3419 punct ;,levels
R395 T3357 T3358 compound %,identity
R396 T3430 T3431 quantmod 10,50
R397 T3431 T3433 nummod 50,μM
R398 T3358 T3355 dobj identity,share
R399 T3432 T3431 punct –,50
R400 T3433 T3434 compound μM,Ca2
R401 T3434 T3419 appos Ca2,levels
R402 T3359 T3360 compound amino,acid
R403 T3435 T3434 punct +,Ca2
R404 T3436 T3434 prep for,Ca2
R405 T3437 T3438 compound μ,calpain
R406 T3360 T3358 compound acid,identity
R407 T3438 T3436 pobj calpain,for
R408 T3439 T3438 punct -,calpain
R409 T3440 T3434 cc and,Ca2
R410 T3361 T3358 compound sequence,identity
R411 T3441 T3442 quantmod 0.3,0.35
R412 T3442 T3444 nummod 0.35,mM
R413 T3443 T3442 punct –,0.35
R414 T3362 T3355 punct ", ",share
R415 T3444 T3445 compound mM,Ca2
R416 T3445 T3434 conj Ca2,Ca2
R417 T3363 T3355 cc and,share
R418 T3446 T3445 punct +,Ca2
R419 T3447 T3445 prep for,Ca2
R420 T3448 T3449 compound m,calpain
R421 T3449 T3447 pobj calpain,for
R422 T3364 T3355 conj are,share
R423 T3450 T3449 punct -,calpain
R424 T3451 T3416 punct .,derive
R425 T3453 T3454 nsubjpass It,assumed
R426 T3365 T3366 advmod very,similar
R427 T3455 T3454 auxpass is,assumed
R428 T3456 T3454 advmod generally,assumed
R429 T3457 T3458 mark that,maintain
R430 T3366 T3364 acomp similar,are
R431 T3458 T3454 ccomp maintain,assumed
R432 T3459 T3460 nmod μ,calpain
R433 T3367 T3364 prep in,are
R434 T3460 T3458 nsubj calpain,maintain
R435 T3461 T3459 punct -,μ
R436 T3462 T3459 cc and,μ
R437 T3463 T3459 conj m,μ
R438 T3368 T3367 pobj terms,in
R439 T3464 T3460 punct -,calpain
R440 T3465 T3466 poss their,sensitivities
R441 T3466 T3458 dobj sensitivities,maintain
R442 T3369 T3368 prep of,terms
R443 T3467 T3466 amod differential,sensitivities
R444 T3468 T3466 prep to,sensitivities
R445 T3370 T3369 pobj structure,of
R446 T3469 T3468 pobj calcium,to
R447 T3470 T3471 advmod in,vivo
R448 T3471 T3458 advmod vivo,maintain
R449 T3371 T3370 punct ", ",structure
R450 T3472 T3458 punct ", ",maintain
R451 T3473 T3474 mark although,demonstrated
R452 T3372 T3373 compound protein,chemistry
R453 T3373 T3370 conj chemistry,structure
R454 T3474 T3458 advcl demonstrated,maintain
R455 T3475 T3474 nsubjpass this,demonstrated
R456 T3476 T3474 aux has,demonstrated
R457 T3374 T3373 punct ", ",chemistry
R458 T3477 T3474 neg not,demonstrated
R459 T3478 T3474 advmod yet,demonstrated
R460 T3375 T3373 cc and,chemistry
R461 T3479 T3474 auxpass been,demonstrated
R462 T3480 T3474 advmod strictly,demonstrated
R463 T3481 T3454 punct .,assumed
R464 T3376 T3377 advmod in,vitro
R465 T3483 T3484 advmod Furthermore,assumed
R466 T3377 T3378 amod vitro,specificity
R467 T3485 T3484 punct ", ",assumed
R468 T3486 T3487 mark since,is
R469 T3378 T3373 conj specificity,chemistry
R470 T3487 T3484 advcl is,assumed
R471 T3488 T3489 det the,concentration
R472 T3379 T3378 compound substrate,specificity
R473 T3489 T3487 nsubj concentration,is
R474 T3490 T3491 amod cytoplasmic,free
R475 T3491 T3489 amod free,concentration
R476 T3380 T3355 punct .,share
R477 T3492 T3489 nmod Ca2,concentration
R478 T3493 T3492 punct +,Ca2
R479 T3494 T3487 advmod typically,is
R480 T3495 T3496 amod less,1
R481 T3496 T3498 nummod 1,μM
R482 T3497 T3496 quantmod than,1
R483 T3382 T3383 prep Despite,suggest
R484 T3498 T3487 attr μM,is
R485 T3499 T3484 punct ", ",assumed
R486 T3500 T3484 nsubjpass it,assumed
R487 T3501 T3484 auxpass is,assumed
R488 T3384 T3385 det these,similarities
R489 T3502 T3484 advmod also,assumed
R490 T3503 T3504 mark that,contribute
R491 T3504 T3484 ccomp contribute,assumed
R492 T3505 T3506 amod other,factors
R493 T3506 T3504 nsubj factors,contribute
R494 T3385 T3382 pobj similarities,Despite
R495 T3507 T3508 advmod in,vivo
R496 T3508 T3506 amod vivo,factors
R497 T3509 T3504 aux must,contribute
R498 T3510 T3504 prep to,contribute
R499 T3386 T3383 punct ", ",suggest
R500 T3511 T3510 pobj regulation,to
R501 T3512 T3511 prep of,regulation
R502 T3387 T3388 det the,patterns
R503 T3513 T3514 det these,enzymes
R504 T3514 T3512 pobj enzymes,of
R505 T3515 T3516 punct [,3
R506 T3388 T3383 nsubj patterns,suggest
R507 T3516 T3504 parataxis 3,contribute
R508 T3517 T3516 punct ],3
R509 T3518 T3484 punct .,assumed
R510 T3520 T3521 prep Without,presents
R511 T3389 T3388 amod differential,patterns
R512 T3522 T3523 nmod apriori,knowledge
R513 T3523 T3520 pobj knowledge,Without
R514 T3390 T3388 compound expression,patterns
R515 T3524 T3523 prep of,knowledge
R516 T3525 T3526 det the,factors
R517 T3526 T3524 pobj factors,of
R518 T3391 T3388 prep of,patterns
R519 T3527 T3526 acl regulating,factors
R520 T3528 T3529 compound calpain,activity
R521 T3529 T3527 dobj activity,regulating
R522 T3392 T3393 nmod μ,calpain
R523 T3530 T3526 cc or,factors
R524 T3531 T3532 poss their,substrates
R525 T3532 T3526 conj substrates,factors
R526 T3393 T3391 pobj calpain,of
R527 T3533 T3532 amod relevant,substrates
R528 T3534 T3521 punct ", ",presents
R529 T3394 T3392 punct -,μ
R530 T3535 T3521 nsubj elucidation,presents
R531 T3395 T3392 cc and,μ
R532 T3536 T3535 prep of,elucidation
R533 T3537 T3538 amod biological,functions
R534 T3538 T3536 pobj functions,of
R535 T3539 T3535 prep for,elucidation
R536 T3396 T3392 conj m,μ
R537 T3540 T3539 pobj calpains,for
R538 T3541 T3542 det a,challenge
R539 T3542 T3521 dobj challenge,presents
R540 T3397 T3393 punct -,calpain
R541 T3543 T3521 punct .,presents
R542 T3398 T3388 prep in,patterns
R543 T3545 T3546 nsubj Research,linked
R544 T3547 T3545 prep on,Research
R545 T3399 T3400 amod mammalian,tissues
R546 T3548 T3547 pobj calpains,on
R547 T3549 T3546 aux has,linked
R548 T3550 T3546 dobj them,linked
R549 T3400 T3398 pobj tissues,in
R550 T3551 T3546 prep with,linked
R551 T3552 T3553 det a,variety
R552 T3401 T3402 nsubj they,have
R553 T3553 T3551 pobj variety,with
R554 T3554 T3553 amod wide,variety
R555 T3555 T3553 prep of,variety
R556 T3402 T3383 advcl have,suggest
R557 T3556 T3555 pobj functions,of
R558 T3557 T3553 prep including,variety
R559 T3558 T3559 compound muscle,growth
R560 T3403 T3404 det some,functions
R561 T3559 T3557 pobj growth,including
R562 T3404 T3402 dobj functions,have
R563 T3560 T3559 punct ", ",growth
R564 T3405 T3406 npadvmod isoform,specific
R565 T3561 T3559 conj development,growth
R566 T3562 T3561 punct ", ",development
R567 T3563 T3561 conj degeneration,development
R568 T3406 T3404 amod specific,functions
R569 T3564 T3565 punct (,3
R570 T3407 T3406 cc and,specific
R571 T3565 T3563 parataxis 3,degeneration
R572 T3408 T3406 conj distinct,specific
R573 T3566 T3565 punct ),3
R574 T3567 T3563 punct ", ",degeneration
R575 T3409 T3383 punct .,suggest
R576 T3568 T3569 amod neuronal,growth
R577 T3569 T3563 conj growth,degeneration
R578 T3411 T3412 det The,designations
R579 T3570 T3569 cc and,growth
R580 T3571 T3569 conj neurodegeneration,growth
R581 T3572 T3573 punct [,4
R582 T3573 T3571 parataxis 4,neurodegeneration
R583 T3574 T3573 punct ],4
R584 T3575 T3569 punct ", ",growth
R585 T3576 T3577 compound cell,cycle
R586 T3412 T3416 nsubj designations,derive
R587 T3577 T3578 compound cycle,progression
R588 T3578 T3569 conj progression,growth
R589 T3413 T3412 nmod μ,designations
R590 T3579 T3580 punct [,6
R591 T3580 T3578 parataxis 6,progression
R592 T3581 T3580 nummod 5,6
R593 T3414 T3413 cc and,μ
R594 T3582 T3580 punct ",",6
R595 T3583 T3580 punct ],6
R596 T3584 T3578 punct ", ",progression
R597 T3415 T3413 conj m,μ
R598 T3585 T3586 compound signal,cascades
R599 T3586 T3578 conj cascades,progression
R600 T3587 T3586 acl triggered,cascades
R601 T3417 T3416 prep from,derive
R602 T3588 T3587 agent by,triggered
R603 T3589 T3588 pobj integrins,by
R604 T3590 T3589 cc and,integrins
R605 T3591 T3592 compound growth,factors
R606 T3418 T3419 det the,levels
R607 T3592 T3589 conj factors,integrins
R608 T3593 T3594 punct [,7
R609 T3594 T3587 parataxis 7,triggered
R610 T3419 T3417 pobj levels,from
R611 T3595 T3594 punct ],7
R612 T3596 T3586 punct ", ",cascades
R613 T3597 T3598 compound membrane,protrusion
R614 T3598 T3586 conj protrusion,cascades
R615 T3420 T3419 prep of,levels
R616 T3599 T3600 punct [,8
R617 T3600 T3598 parataxis 8,protrusion
R618 T3421 T3420 pobj Ca2,of
R619 T3601 T3600 punct ],8
R620 T3602 T3598 punct ", ",protrusion
R621 T3603 T3598 conj remodeling,protrusion
R622 T3422 T3421 punct +,Ca2
R623 T3604 T3603 prep of,remodeling
R624 T3605 T3606 det the,cytoskeleton
R625 T3606 T3604 pobj cytoskeleton,of
R626 T3423 T3419 acl required,levels
R627 T3607 T3603 cc and,remodeling
R628 T3608 T3609 compound cell,migration
R629 T3609 T3603 conj migration,remodeling
R630 T3424 T3425 advmod in,vitro
R631 T3610 T3611 punct [,9
R632 T3611 T3609 parataxis 9,migration
R633 T3612 T3613 punct -,15
R634 T3613 T3611 appos 15,9
R635 T3614 T3611 punct ],9
R636 T3615 T3603 punct ", ",remodeling
R637 T3425 T3423 advmod vitro,required
R638 T3616 T3603 cc and,remodeling
R639 T3617 T3603 conj regulation,remodeling
R640 T3426 T3423 prep for,required
R641 T3618 T3617 prep of,regulation
R642 T3619 T3620 compound cell,death
R643 T3620 T3618 pobj death,of
R644 T3621 T3617 prep via,regulation
R645 T3427 T3428 amod optimal,activation
R646 T3622 T3623 preconj both,necrosis
R647 T3623 T3621 pobj necrosis,via
R648 T3624 T3623 cc and,necrosis
R649 T3428 T3426 pobj activation,for
R650 T3625 T3623 conj apoptosis,necrosis
R651 T3626 T3627 punct [,16
R652 T3627 T3617 parataxis 16,regulation
R653 T3640 T3634 dobj interplay,suggests
R654 T3628 T3629 punct -,22
R655 T3629 T3627 appos 22,16
R656 T3630 T3627 punct ],16
R657 T3631 T3546 punct .,linked
R658 T3641 T3640 amod complex,interplay
R659 T3633 T3634 prep To,suggests
R660 T3642 T3640 prep between,interplay
R661 T3635 T3633 pobj date,To
R662 T3636 T3634 punct ", ",suggests
R663 T3643 T3642 pobj caspases,between
R664 T3637 T3638 det the,literature
R665 T3638 T3634 nsubj literature,suggests
R666 T3639 T3640 det a,interplay
R667 T3644 T3643 cc and,caspases
R668 T3645 T3643 conj calpains,caspases
R669 T3646 T3647 punct [,24
R670 T3647 T3640 parataxis 24,interplay
R671 T3746 T3745 punct -,calpain
R672 T3747 T3742 prep in,role
R673 T3648 T3647 nummod 23,24
R674 T3748 T3749 amod epidermal,factor
R675 T3749 T3751 npadvmod factor,induced
R676 T3750 T3749 compound growth,factor
R677 T3649 T3647 punct ",",24
R678 T3751 T3756 amod induced,motility
R679 T3752 T3749 punct (,factor
R680 T3650 T3647 punct ],24
R681 T3753 T3749 appos EGF,factor
R682 T3754 T3751 punct ),induced
R683 T3755 T3751 punct -,induced
R684 T3651 T3640 cc and,interplay
R685 T3756 T3747 pobj motility,in
R686 T3757 T3756 compound cell,motility
R687 T3758 T3759 punct [,29
R688 T3759 T3742 parataxis 29,role
R689 T3760 T3759 nummod 28,29
R690 T3761 T3759 punct ",",29
R691 T3652 T3640 conj impact,interplay
R692 T3762 T3759 punct ],29
R693 T3763 T3742 cc and,role
R694 T3764 T3765 det a,role
R695 T3653 T3652 prep of,impact
R696 T3765 T3742 conj role,role
R697 T3766 T3765 prep for,role
R698 T3654 T3653 pobj calpain,of
R699 T3767 T3768 compound μ,calpain
R700 T3768 T3766 pobj calpain,for
R701 T3769 T3768 punct -,calpain
R702 T3655 T3652 prep on,impact
R703 T3770 T3765 prep in,role
R704 T3771 T3772 npadvmod interferon,inducible
R705 T3772 T3774 amod inducible,migration
R706 T3656 T3657 compound cell,death
R707 T3773 T3772 punct -,inducible
R708 T3774 T3770 pobj migration,in
R709 T3775 T3776 npadvmod protein,induced
R710 T3657 T3658 compound death,components
R711 T3776 T3774 amod induced,migration
R712 T3777 T3775 nummod 9,protein
R713 T3778 T3776 punct -,induced
R714 T3658 T3655 pobj components,on
R715 T3779 T3774 prep of,migration
R716 T3780 T3779 pobj keratinocytes,of
R717 T3781 T3782 punct [,28
R718 T3659 T3658 compound pathway,components
R719 T3782 T3765 parataxis 28,role
R720 T3783 T3782 punct ],28
R721 T3784 T3733 punct .,suggested
R722 T3660 T3661 punct [,25
R723 T3786 T3787 det A,inhibitor
R724 T3661 T3652 parataxis 25,impact
R725 T3787 T3791 nsubjpass inhibitor,used
R726 T3788 T3789 npadvmod cell,permeable
R727 T3789 T3787 amod permeable,inhibitor
R728 T3790 T3787 compound calpain,inhibitor
R729 T3662 T3661 punct ],25
R730 T3792 T3787 punct (,inhibitor
R731 T3663 T3634 punct .,suggests
R732 T3793 T3794 dep which,inhibits
R733 T3794 T3787 relcl inhibits,inhibitor
R734 T3795 T3794 advmod likely,inhibits
R735 T3665 T3666 det The,lack
R736 T3796 T3797 amod other,proteases
R737 T3797 T3794 dobj proteases,inhibits
R738 T3798 T3797 compound thiol,proteases
R739 T3799 T3797 punct -,proteases
R740 T3800 T3801 advmod as,well
R741 T3801 T3794 advmod well,inhibits
R742 T3802 T3791 punct ),used
R743 T3666 T3667 nsubj lack,contributes
R744 T3803 T3791 aux has,used
R745 T3804 T3791 auxpass been,used
R746 T3805 T3806 aux to,select
R747 T3668 T3666 prep of,lack
R748 T3806 T3791 advcl select,used
R749 T3807 T3806 dobj cells,select
R750 T3808 T3807 acl lacking,cells
R751 T3809 T3810 compound μ,calpain
R752 T3669 T3670 advmod highly,specific
R753 T3670 T3671 amod specific,inhibitors
R754 T3810 T3808 dobj calpain,lacking
R755 T3671 T3668 pobj inhibitors,of
R756 T3811 T3810 punct -,calpain
R757 T3812 T3813 dep which,display
R758 T3813 T3807 relcl display,cells
R759 T3672 T3673 npadvmod cell,permeable
R760 T3814 T3815 amod reduced,rates
R761 T3815 T3813 dobj rates,display
R762 T3816 T3815 compound proliferation,rates
R763 T3673 T3671 amod permeable,inhibitors
R764 T3817 T3818 punct [,30
R765 T3818 T3813 parataxis 30,display
R766 T3819 T3818 punct ],30
R767 T3674 T3673 punct -,permeable
R768 T3820 T3791 punct .,used
R769 T3675 T3671 prep of,inhibitors
R770 T3822 T3823 advmod Interestingly,persisted
R771 T3676 T3675 pobj calpains,of
R772 T3824 T3823 punct ", ",persisted
R773 T3825 T3826 compound m,calpain
R774 T3826 T3828 compound calpain,expression
R775 T3677 T3667 prep to,contributes
R776 T3827 T3826 punct -,calpain
R777 T3828 T3823 nsubj expression,persisted
R778 T3829 T3823 prep in,persisted
R779 T3678 T3679 det the,challenge
R780 T3830 T3831 det these,cells
R781 T3831 T3829 pobj cells,in
R782 T3832 T3823 punct ", ",persisted
R783 T3679 T3677 pobj challenge,to
R784 T3833 T3823 advcl suggesting,persisted
R785 T3834 T3835 det a,requirement
R786 T3835 T3833 dobj requirement,suggesting
R787 T3680 T3679 prep of,challenge
R788 T3836 T3835 amod possible,requirement
R789 T3837 T3835 prep of,requirement
R790 T3681 T3680 pcomp investigating,of
R791 T3838 T3839 compound m,calpain
R792 T3839 T3837 pobj calpain,of
R793 T3840 T3839 punct -,calpain
R794 T3841 T3835 prep for,requirement
R795 T3842 T3843 compound cell,survival
R796 T3843 T3841 pobj survival,for
R797 T3682 T3681 cc and,investigating
R798 T3844 T3845 punct [,30
R799 T3845 T3823 parataxis 30,persisted
R800 T3846 T3845 punct ],30
R801 T3683 T3681 conj defining,investigating
R802 T3847 T3823 punct .,persisted
R803 T3684 T3685 compound calpain,functions
R804 T3849 T3850 amod Targeted,deletion
R805 T3685 T3683 dobj functions,defining
R806 T3686 T3683 prep in,defining
R807 T3850 T3852 nsubj deletion,provides
R808 T3687 T3688 det these,processes
R809 T3851 T3850 compound gene,deletion
R810 T3853 T3850 prep in,deletion
R811 T3688 T3686 pobj processes,in
R812 T3854 T3853 pobj mice,in
R813 T3855 T3856 det a,approach
R814 T3689 T3667 punct .,contributes
R815 T3856 T3852 dobj approach,provides
R816 T3691 T3692 mark Although,provides
R817 T3857 T3856 amod powerful,approach
R818 T3858 T3856 prep to,approach
R819 T3859 T3858 pcomp determining,to
R820 T3860 T3861 det the,roles
R821 T3692 T3708 advcl provides,distinguish
R822 T3861 T3859 dobj roles,determining
R823 T3862 T3861 amod physiological,roles
R824 T3863 T3861 prep of,roles
R825 T3693 T3692 nsubj over-expression,provides
R826 T3864 T3865 nmod μ,calpain
R827 T3865 T3863 pobj calpain,of
R828 T3694 T3693 prep of,over-expression
R829 T3866 T3864 punct -,μ
R830 T3867 T3864 cc and,μ
R831 T3868 T3864 conj m,μ
R832 T3695 T3694 pobj calpastatin,of
R833 T3869 T3865 punct -,calpain
R834 T3870 T3856 cc and,approach
R835 T3871 T3872 det the,opportunity
R836 T3696 T3695 punct ", ",calpastatin
R837 T3872 T3856 conj opportunity,approach
R838 T3873 T3874 aux to,approach
R839 T3697 T3698 det the,inhibitor
R840 T3874 T3872 acl approach,opportunity
R841 T3875 T3876 poss their,functions
R842 T3876 T3874 dobj functions,approach
R843 T3877 T3878 npadvmod isoform,specific
R844 T3878 T3876 amod specific,functions
R845 T3879 T3852 punct .,provides
R846 T3698 T3695 appos inhibitor,calpastatin
R849 T3699 T3698 amod endogenous,inhibitor
R852 T3700 T3698 compound protein,inhibitor
R857 T3701 T3698 prep of,inhibitor
R859 T3702 T3703 nmod μ,calpain
R864 T3703 T3701 pobj calpain,of
R867 T3704 T3702 punct -,μ
R869 T3705 T3702 cc and,μ
R872 T3706 T3702 conj m,μ
R875 T3707 T3703 punct -,calpain
R879 T3709 T3710 det an,approach
R883 T3710 T3692 dobj approach,provides
R887 T3711 T3710 amod important,approach
R890 T3712 T3710 prep for,approach
R893 T3713 T3714 det these,efforts
R900 T3714 T3712 pobj efforts,for
R904 T3715 T3708 punct ", ",distinguish
R908 T3716 T3708 nsubj it,distinguish
R912 T3717 T3708 aux will,distinguish
R915 T3718 T3708 neg not,distinguish
R920 T3719 T3720 npadvmod isoform,specific
R923 T3720 T3721 amod specific,functions
R927 T3721 T3708 dobj functions,distinguish
R930 T3722 T3723 punct [,27
R933 T3723 T3708 parataxis 27,distinguish
R936 T3724 T3723 nummod 24,27
R937 T3725 T3723 punct ",",27
R939 T3726 T3723 nummod 26,27
R941 T3727 T3723 punct ",",27
R944 T3728 T3723 punct ],27
R945 T3729 T3708 punct .,distinguish
R947 T3731 T3732 det Some,work
R955 T3732 T3733 nsubj work,suggested
R959 T3734 T3733 aux has,suggested
R964 T3735 T3736 npadvmod isoform,specific
R968 T3736 T3737 amod specific,roles
R971 T3737 T3733 dobj roles,suggested
R974 T3738 T3737 punct ", ",roles
R978 T3739 T3740 amod such,as
R981 T3740 T3737 prep as,roles
R982 T3741 T3742 det a,role
R985 T3742 T3740 pobj role,as
R989 T3743 T3742 prep for,role
R993 T3744 T3745 compound m,calpain
R996 T3745 T3743 pobj calpain,for