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PMC:1315279 / 41916-42738 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T9732 0-10 NN denotes Genotyping
T9733 11-14 CC denotes and
T9734 15-25 NN denotes immunoblot
T9735 26-34 NNS denotes analyses
T9736 34-35 . denotes .
T9737 35-174 sentence denotes All mouse procedures were performed in accordance with McGill University guidelines, which are set by the Canadian Council on Animal Care.
T9738 36-39 DT denotes All
T9740 40-45 NN denotes mouse
T9739 46-56 NNS denotes procedures
T9742 57-61 VBD denotes were
T9741 62-71 VBN denotes performed
T9743 72-74 IN denotes in
T9744 75-85 NN denotes accordance
T9745 86-90 IN denotes with
T9746 91-97 NNP denotes McGill
T9747 98-108 NNP denotes University
T9748 109-119 NNS denotes guidelines
T9749 119-121 , denotes ,
T9750 121-126 WDT denotes which
T9752 127-130 VBP denotes are
T9751 131-134 VBN denotes set
T9753 135-137 IN denotes by
T9754 138-141 DT denotes the
T9756 142-150 NNP denotes Canadian
T9755 151-158 NNP denotes Council
T9757 159-161 IN denotes on
T9758 162-168 NNP denotes Animal
T9759 169-173 NNP denotes Care
T9760 173-174 . denotes .
T9761 174-278 sentence denotes Genomic DNA was isolated from tail biopsies and analyzed by Southern blotting and genomic PCR analysis.
T9762 175-182 JJ denotes Genomic
T9763 183-186 NN denotes DNA
T9765 187-190 VBD denotes was
T9764 191-199 VBN denotes isolated
T9766 200-204 IN denotes from
T9767 205-209 NN denotes tail
T9768 210-218 NNS denotes biopsies
T9769 219-222 CC denotes and
T9770 223-231 VBN denotes analyzed
T9771 232-234 IN denotes by
T9772 235-243 NNP denotes Southern
T9773 244-252 NN denotes blotting
T9774 253-256 CC denotes and
T9775 257-264 JJ denotes genomic
T9777 265-268 NN denotes PCR
T9776 269-277 NN denotes analysis
T9778 277-278 . denotes .
T9779 278-480 sentence denotes The DNA fragment utilized as the probe for the Southern blotting analysis was amplified with the following two oligonucleotides (5′-AAG CCT TTA CTG GTT GTG T-3′) and (5′-CTT GAA ACG CAC CGT AGG CT-3′).
T9780 279-282 DT denotes The
T9782 283-286 NN denotes DNA
T9781 287-295 NN denotes fragment
T9784 296-304 VBN denotes utilized
T9785 305-307 IN denotes as
T9786 308-311 DT denotes the
T9787 312-317 NN denotes probe
T9788 318-321 IN denotes for
T9789 322-325 DT denotes the
T9791 326-334 NNP denotes Southern
T9792 335-343 NN denotes blotting
T9790 344-352 NN denotes analysis
T9793 353-356 VBD denotes was
T9783 357-366 VBN denotes amplified
T9794 367-371 IN denotes with
T9795 372-375 DT denotes the
T9797 376-385 VBG denotes following
T9798 386-389 CD denotes two
T9796 390-406 NNS denotes oligonucleotides
T9799 407-408 -LRB- denotes (
T9800 408-409 CD denotes 5
T9801 409-410 SYM denotes ′
T9802 410-411 HYPH denotes -
T9803 411-414 NN denotes AAG
T9805 415-418 NN denotes CCT
T9806 419-422 NN denotes TTA
T9807 423-426 NN denotes CTG
T9808 427-430 NN denotes GTT
T9809 431-434 NN denotes GTG
T9804 435-436 NN denotes T
T9810 436-437 HYPH denotes -
T9811 437-438 CD denotes 3
T9812 438-439 SYM denotes ′
T9813 439-440 -RRB- denotes )
T9814 441-444 CC denotes and
T9815 445-446 -LRB- denotes (
T9816 446-447 CD denotes 5
T9817 447-448 SYM denotes ′
T9818 448-449 HYPH denotes -
T9819 449-452 NN denotes CTT
T9821 453-456 NN denotes GAA
T9822 457-460 NN denotes ACG
T9823 461-464 NN denotes CAC
T9824 465-468 NN denotes CGT
T9825 469-472 NN denotes AGG
T9820 473-475 NN denotes CT
T9826 475-476 HYPH denotes -
T9827 476-477 CD denotes 3
T9828 477-478 SYM denotes ′
T9829 478-479 -RRB- denotes )
T9830 479-480 . denotes .
T9831 480-654 sentence denotes The wild-type sam68 allele was identified by genomic PCR using the following oligonucleotides 5′-AAA TCC TAA CCC TCC TCA GTC AG-3′ and 5′-GAT ATG ATG GAT GAT ATC TGT CAG-3′.
T9832 481-484 DT denotes The
T9834 485-489 JJ denotes wild
T9836 489-490 HYPH denotes -
T9835 490-494 NN denotes type
T9837 495-500 NN denotes sam68
T9833 501-507 NN denotes allele
T9839 508-511 VBD denotes was
T9838 512-522 VBN denotes identified
T9840 523-525 IN denotes by
T9841 526-533 JJ denotes genomic
T9842 534-537 NN denotes PCR
T9843 538-543 VBG denotes using
T9844 544-547 DT denotes the
T9846 548-557 VBG denotes following
T9845 558-574 NNS denotes oligonucleotides
T9847 575-576 CD denotes 5
T9848 576-577 SYM denotes ′
T9849 577-578 HYPH denotes -
T9850 578-581 NN denotes AAA
T9852 582-585 NN denotes TCC
T9853 586-589 NN denotes TAA
T9854 590-593 NN denotes CCC
T9855 594-597 NN denotes TCC
T9856 598-601 NN denotes TCA
T9857 602-605 NN denotes GTC
T9851 606-608 NN denotes AG
T9858 608-609 HYPH denotes -
T9859 609-610 CD denotes 3
T9860 610-611 SYM denotes ′
T9861 612-615 CC denotes and
T9862 616-617 CD denotes 5
T9863 617-618 SYM denotes ′
T9864 618-619 HYPH denotes -
T9865 619-622 NN denotes GAT
T9867 623-626 NN denotes ATG
T9868 627-630 NN denotes ATG
T9869 631-634 NN denotes GAT
T9870 635-638 NN denotes GAT
T9871 639-642 NN denotes ATC
T9872 643-646 NN denotes TGT
T9866 647-650 NN denotes CAG
T9873 650-651 HYPH denotes -
T9874 651-652 CD denotes 3
T9875 652-653 SYM denotes ′
T9876 653-654 . denotes .
T9877 654-822 sentence denotes The sam68-targeted allele was identified by genomic PCR using the following oligonucleotides 5′-CTT GGG TGG AGA GGC TAT TCG-3′ and 5′-GTC GGG CAT GCG CGC CTT GAG C-3′.
T9878 655-658 DT denotes The
T9880 659-664 NN denotes sam68
T9882 664-665 HYPH denotes -
T9881 665-673 VBN denotes targeted
T9879 674-680 NN denotes allele
T9884 681-684 VBD denotes was
T9883 685-695 VBN denotes identified
T9885 696-698 IN denotes by
T9886 699-706 JJ denotes genomic
T9887 707-710 NN denotes PCR
T9888 711-716 VBG denotes using
T9889 717-720 DT denotes the
T9891 721-730 VBG denotes following
T9890 731-747 NNS denotes oligonucleotides
T9892 748-749 CD denotes 5
T9893 749-750 SYM denotes ′
T9894 750-751 HYPH denotes -
T9895 751-754 NN denotes CTT
T9897 755-758 NN denotes GGG
T9898 759-762 NN denotes TGG
T9899 763-766 NN denotes AGA
T9900 767-770 NN denotes GGC
T9901 771-774 NN denotes TAT
T9896 775-778 NN denotes TCG
T9902 778-779 HYPH denotes -
T9903 779-780 CD denotes 3
T9904 780-781 SYM denotes ′
T9905 782-785 CC denotes and
T9906 786-787 CD denotes 5
T9907 787-788 SYM denotes ′
T9908 788-789 HYPH denotes -
T9909 789-792 NN denotes GTC
T9911 793-796 NN denotes GGG
T9912 797-800 NN denotes CAT
T9913 801-804 NN denotes GCG
T9914 805-808 NN denotes CGC
T9915 809-812 NN denotes CTT
T9916 813-816 NN denotes GAG
T9910 817-818 NN denotes C
T9917 818-819 HYPH denotes -
T9918 819-820 CD denotes 3
T9919 820-821 SYM denotes ′
T9920 821-822 . denotes .
R6154 T9733 T9732 cc and,Genotyping
R6155 T9734 T9735 compound immunoblot,analyses
R6156 T9735 T9732 conj analyses,Genotyping
R6157 T9736 T9735 punct .,analyses
R6158 T9738 T9739 det All,procedures
R6159 T9739 T9741 nsubjpass procedures,performed
R6160 T9740 T9739 compound mouse,procedures
R6161 T9742 T9741 auxpass were,performed
R6162 T9743 T9741 prep in,performed
R6163 T9744 T9743 pobj accordance,in
R6164 T9745 T9744 prep with,accordance
R6165 T9746 T9747 compound McGill,University
R6166 T9747 T9748 compound University,guidelines
R6167 T9748 T9745 pobj guidelines,with
R6168 T9749 T9748 punct ", ",guidelines
R6169 T9750 T9751 dep which,set
R6170 T9751 T9748 relcl set,guidelines
R6171 T9752 T9751 auxpass are,set
R6172 T9753 T9751 agent by,set
R6173 T9754 T9755 det the,Council
R6174 T9755 T9753 pobj Council,by
R6175 T9756 T9755 compound Canadian,Council
R6176 T9757 T9755 prep on,Council
R6177 T9758 T9759 compound Animal,Care
R6178 T9759 T9757 pobj Care,on
R6179 T9760 T9741 punct .,performed
R6180 T9762 T9763 amod Genomic,DNA
R6181 T9763 T9764 nsubjpass DNA,isolated
R6182 T9765 T9764 auxpass was,isolated
R6183 T9766 T9764 prep from,isolated
R6184 T9767 T9768 compound tail,biopsies
R6185 T9768 T9766 pobj biopsies,from
R6186 T9769 T9764 cc and,isolated
R6187 T9770 T9764 conj analyzed,isolated
R6188 T9771 T9770 prep by,analyzed
R6189 T9772 T9773 compound Southern,blotting
R6190 T9773 T9771 pobj blotting,by
R6191 T9774 T9773 cc and,blotting
R6192 T9775 T9776 amod genomic,analysis
R6193 T9776 T9773 conj analysis,blotting
R6194 T9777 T9776 compound PCR,analysis
R6195 T9778 T9764 punct .,isolated
R6196 T9780 T9781 det The,fragment
R6197 T9781 T9783 nsubjpass fragment,amplified
R6198 T9782 T9781 compound DNA,fragment
R6199 T9784 T9781 acl utilized,fragment
R6200 T9785 T9784 prep as,utilized
R6201 T9786 T9787 det the,probe
R6202 T9787 T9785 pobj probe,as
R6203 T9788 T9787 prep for,probe
R6204 T9789 T9790 det the,analysis
R6205 T9790 T9788 pobj analysis,for
R6206 T9791 T9792 compound Southern,blotting
R6207 T9792 T9790 compound blotting,analysis
R6208 T9793 T9783 auxpass was,amplified
R6209 T9794 T9783 prep with,amplified
R6210 T9795 T9796 det the,oligonucleotides
R6211 T9796 T9794 pobj oligonucleotides,with
R6212 T9797 T9796 amod following,oligonucleotides
R6213 T9798 T9796 nummod two,oligonucleotides
R6214 T9799 T9796 punct (,oligonucleotides
R6215 T9800 T9796 appos 5,oligonucleotides
R6216 T9801 T9800 punct ′,5
R6217 T9802 T9800 punct -,5
R6218 T9803 T9804 compound AAG,T
R6219 T9804 T9800 appos T,5
R6220 T9805 T9804 compound CCT,T
R6221 T9806 T9804 compound TTA,T
R6222 T9807 T9804 compound CTG,T
R6223 T9808 T9804 compound GTT,T
R6224 T9809 T9804 compound GTG,T
R6225 T9810 T9800 punct -,5
R6226 T9811 T9800 nummod 3,5
R6227 T9812 T9800 punct ′,5
R6228 T9813 T9800 punct ),5
R6229 T9814 T9800 cc and,5
R6230 T9815 T9816 punct (,5
R6231 T9816 T9800 conj 5,5
R6232 T9817 T9816 punct ′,5
R6233 T9818 T9816 punct -,5
R6234 T9819 T9820 compound CTT,CT
R6235 T9820 T9816 appos CT,5
R6236 T9821 T9820 compound GAA,CT
R6237 T9822 T9820 compound ACG,CT
R6238 T9823 T9820 compound CAC,CT
R6239 T9824 T9820 compound CGT,CT
R6240 T9825 T9820 compound AGG,CT
R6241 T9826 T9816 punct -,5
R6242 T9827 T9816 nummod 3,5
R6243 T9828 T9816 punct ′,5
R6244 T9829 T9783 punct ),amplified
R6245 T9830 T9783 punct .,amplified
R6246 T9832 T9833 det The,allele
R6247 T9833 T9838 nsubjpass allele,identified
R6248 T9834 T9835 amod wild,type
R6249 T9835 T9833 compound type,allele
R6250 T9836 T9835 punct -,type
R6251 T9837 T9833 compound sam68,allele
R6252 T9839 T9838 auxpass was,identified
R6253 T9840 T9838 prep by,identified
R6254 T9841 T9842 amod genomic,PCR
R6255 T9842 T9840 pobj PCR,by
R6256 T9843 T9838 advcl using,identified
R6257 T9844 T9845 det the,oligonucleotides
R6258 T9845 T9843 dobj oligonucleotides,using
R6259 T9846 T9845 amod following,oligonucleotides
R6260 T9847 T9845 appos 5,oligonucleotides
R6261 T9848 T9847 punct ′,5
R6262 T9849 T9847 punct -,5
R6263 T9850 T9851 compound AAA,AG
R6264 T9851 T9847 appos AG,5
R6265 T9852 T9851 compound TCC,AG
R6266 T9853 T9851 compound TAA,AG
R6267 T9854 T9851 compound CCC,AG
R6268 T9855 T9851 compound TCC,AG
R6269 T9856 T9851 compound TCA,AG
R6270 T9857 T9851 compound GTC,AG
R6271 T9858 T9847 punct -,5
R6272 T9859 T9847 nummod 3,5
R6273 T9860 T9847 punct ′,5
R6274 T9861 T9847 cc and,5
R6275 T9862 T9847 conj 5,5
R6276 T9863 T9862 punct ′,5
R6277 T9864 T9862 punct -,5
R6278 T9865 T9866 compound GAT,CAG
R6279 T9866 T9862 appos CAG,5
R6280 T9867 T9866 compound ATG,CAG
R6281 T9868 T9866 compound ATG,CAG
R6282 T9869 T9866 compound GAT,CAG
R6283 T9870 T9866 compound GAT,CAG
R6284 T9871 T9866 compound ATC,CAG
R6285 T9872 T9866 compound TGT,CAG
R6286 T9873 T9862 punct -,5
R6287 T9874 T9862 nummod 3,5
R6288 T9875 T9862 punct ′,5
R6289 T9876 T9838 punct .,identified
R6290 T9878 T9879 det The,allele
R6291 T9879 T9883 nsubjpass allele,identified
R6292 T9880 T9881 npadvmod sam68,targeted
R6293 T9881 T9879 amod targeted,allele
R6294 T9882 T9881 punct -,targeted
R6295 T9884 T9883 auxpass was,identified
R6296 T9885 T9883 prep by,identified
R6297 T9886 T9887 amod genomic,PCR
R6298 T9887 T9885 pobj PCR,by
R6299 T9888 T9883 advcl using,identified
R6300 T9889 T9890 det the,oligonucleotides
R6301 T9890 T9888 dobj oligonucleotides,using
R6302 T9891 T9890 amod following,oligonucleotides
R6303 T9892 T9890 appos 5,oligonucleotides
R6304 T9893 T9892 punct ′,5
R6305 T9894 T9892 punct -,5
R6306 T9895 T9896 compound CTT,TCG
R6307 T9896 T9892 appos TCG,5
R6308 T9897 T9896 compound GGG,TCG
R6309 T9898 T9896 compound TGG,TCG
R6310 T9899 T9896 compound AGA,TCG
R6311 T9900 T9896 compound GGC,TCG
R6312 T9901 T9896 compound TAT,TCG
R6313 T9902 T9892 punct -,5
R6314 T9903 T9892 nummod 3,5
R6315 T9904 T9892 punct ′,5
R6316 T9905 T9892 cc and,5
R6317 T9906 T9892 conj 5,5
R6318 T9907 T9906 punct ′,5
R6319 T9908 T9906 punct -,5
R6320 T9909 T9910 compound GTC,C
R6321 T9910 T9906 appos C,5
R6322 T9911 T9910 compound GGG,C
R6323 T9912 T9910 compound CAT,C
R6324 T9913 T9910 compound GCG,C
R6325 T9914 T9910 compound CGC,C
R6326 T9915 T9910 compound CTT,C
R6327 T9916 T9910 compound GAG,C
R6328 T9917 T9906 punct -,5
R6329 T9918 T9906 nummod 3,5
R6330 T9919 T9906 punct ′,5
R6331 T9920 T9883 punct .,identified