Id |
Subject |
Object |
Predicate |
Lexical cue |
T8863 |
0-10 |
JJ |
denotes |
Histologic |
T8865 |
10-12 |
, |
denotes |
, |
T8866 |
12-31 |
JJ |
denotes |
immunohistochemical |
T8867 |
31-33 |
, |
denotes |
, |
T8868 |
33-36 |
CC |
denotes |
and |
T8869 |
37-54 |
JJ |
denotes |
histomorphometric |
T8864 |
55-63 |
NNS |
denotes |
analyses |
T8870 |
63-64 |
. |
denotes |
. |
T8871 |
64-137 |
sentence |
denotes |
All analyses were performed essentially as described previously [62,63]. |
T8872 |
65-68 |
DT |
denotes |
All |
T8873 |
69-77 |
NNS |
denotes |
analyses |
T8875 |
78-82 |
VBD |
denotes |
were |
T8874 |
83-92 |
VBN |
denotes |
performed |
T8876 |
93-104 |
RB |
denotes |
essentially |
T8878 |
105-107 |
IN |
denotes |
as |
T8877 |
108-117 |
VBN |
denotes |
described |
T8879 |
118-128 |
RB |
denotes |
previously |
T8880 |
129-130 |
-LRB- |
denotes |
[ |
T8882 |
130-132 |
CD |
denotes |
62 |
T8883 |
132-133 |
, |
denotes |
, |
T8881 |
133-135 |
CD |
denotes |
63 |
T8884 |
135-136 |
-RRB- |
denotes |
] |
T8885 |
136-137 |
. |
denotes |
. |
T8886 |
137-332 |
sentence |
denotes |
Briefly, embryonic mice were removed from timed pregnant dams at E14.5 and E16.5 and fixed intact for 36 h in 4% paraformaldehyde, rinsed thoroughly in PBS, and processed for paraffin embedding. |
T8887 |
138-145 |
RB |
denotes |
Briefly |
T8889 |
145-147 |
, |
denotes |
, |
T8890 |
147-156 |
JJ |
denotes |
embryonic |
T8891 |
157-161 |
NNS |
denotes |
mice |
T8892 |
162-166 |
VBD |
denotes |
were |
T8888 |
167-174 |
VBN |
denotes |
removed |
T8893 |
175-179 |
IN |
denotes |
from |
T8894 |
180-185 |
VBN |
denotes |
timed |
T8896 |
186-194 |
JJ |
denotes |
pregnant |
T8895 |
195-199 |
NNS |
denotes |
dams |
T8897 |
200-202 |
IN |
denotes |
at |
T8898 |
203-208 |
NN |
denotes |
E14.5 |
T8899 |
209-212 |
CC |
denotes |
and |
T8900 |
213-218 |
NN |
denotes |
E16.5 |
T8901 |
219-222 |
CC |
denotes |
and |
T8902 |
223-228 |
VBN |
denotes |
fixed |
T8903 |
229-235 |
JJ |
denotes |
intact |
T8904 |
236-239 |
IN |
denotes |
for |
T8905 |
240-242 |
CD |
denotes |
36 |
T8906 |
243-244 |
NN |
denotes |
h |
T8907 |
245-247 |
IN |
denotes |
in |
T8908 |
248-249 |
CD |
denotes |
4 |
T8909 |
249-250 |
NN |
denotes |
% |
T8910 |
251-267 |
NN |
denotes |
paraformaldehyde |
T8911 |
267-269 |
, |
denotes |
, |
T8912 |
269-275 |
VBN |
denotes |
rinsed |
T8913 |
276-286 |
RB |
denotes |
thoroughly |
T8914 |
287-289 |
IN |
denotes |
in |
T8915 |
290-293 |
NN |
denotes |
PBS |
T8916 |
293-295 |
, |
denotes |
, |
T8917 |
295-298 |
CC |
denotes |
and |
T8918 |
299-308 |
VBN |
denotes |
processed |
T8919 |
309-312 |
IN |
denotes |
for |
T8920 |
313-321 |
NN |
denotes |
paraffin |
T8921 |
322-331 |
NN |
denotes |
embedding |
T8922 |
331-332 |
. |
denotes |
. |
T8923 |
332-587 |
sentence |
denotes |
Serial 4-μm sections were cut on a modified Leica RM 2155 rotary microtome (Leica Microsystems, Richmond Hill, Ontario, Canada), stained with a 1:600 dilution of the AD1 anti-Sam68 antibody [46] and counterstained with either methyl green or hematoxylin. |
T8924 |
333-339 |
JJ |
denotes |
Serial |
T8926 |
340-341 |
CD |
denotes |
4 |
T8928 |
341-342 |
HYPH |
denotes |
- |
T8927 |
342-344 |
NN |
denotes |
μm |
T8925 |
345-353 |
NNS |
denotes |
sections |
T8930 |
354-358 |
VBD |
denotes |
were |
T8929 |
359-362 |
VBN |
denotes |
cut |
T8931 |
363-365 |
IN |
denotes |
on |
T8932 |
366-367 |
DT |
denotes |
a |
T8934 |
368-376 |
VBN |
denotes |
modified |
T8935 |
377-382 |
NNP |
denotes |
Leica |
T8936 |
383-385 |
NNP |
denotes |
RM |
T8937 |
386-390 |
CD |
denotes |
2155 |
T8938 |
391-397 |
JJ |
denotes |
rotary |
T8933 |
398-407 |
NN |
denotes |
microtome |
T8939 |
408-409 |
-LRB- |
denotes |
( |
T8941 |
409-414 |
NNP |
denotes |
Leica |
T8940 |
415-427 |
NNP |
denotes |
Microsystems |
T8942 |
427-429 |
, |
denotes |
, |
T8943 |
429-437 |
NNP |
denotes |
Richmond |
T8944 |
438-442 |
NNP |
denotes |
Hill |
T8945 |
442-444 |
, |
denotes |
, |
T8946 |
444-451 |
NNP |
denotes |
Ontario |
T8947 |
451-453 |
, |
denotes |
, |
T8948 |
453-459 |
NNP |
denotes |
Canada |
T8949 |
459-460 |
-RRB- |
denotes |
) |
T8950 |
460-462 |
, |
denotes |
, |
T8951 |
462-469 |
VBN |
denotes |
stained |
T8952 |
470-474 |
IN |
denotes |
with |
T8953 |
475-476 |
DT |
denotes |
a |
T8955 |
477-478 |
CD |
denotes |
1 |
T8956 |
478-479 |
SYM |
denotes |
: |
T8957 |
479-482 |
CD |
denotes |
600 |
T8954 |
483-491 |
NN |
denotes |
dilution |
T8958 |
492-494 |
IN |
denotes |
of |
T8959 |
495-498 |
DT |
denotes |
the |
T8961 |
499-502 |
NN |
denotes |
AD1 |
T8962 |
503-513 |
JJ |
denotes |
anti-Sam68 |
T8960 |
514-522 |
NN |
denotes |
antibody |
T8963 |
523-524 |
-LRB- |
denotes |
[ |
T8964 |
524-526 |
CD |
denotes |
46 |
T8965 |
526-527 |
-RRB- |
denotes |
] |
T8966 |
528-531 |
CC |
denotes |
and |
T8967 |
532-546 |
VBN |
denotes |
counterstained |
T8968 |
547-551 |
IN |
denotes |
with |
T8969 |
552-558 |
CC |
denotes |
either |
T8971 |
559-565 |
NN |
denotes |
methyl |
T8970 |
566-571 |
NN |
denotes |
green |
T8972 |
572-574 |
CC |
denotes |
or |
T8973 |
575-586 |
NN |
denotes |
hematoxylin |
T8974 |
586-587 |
. |
denotes |
. |
T8975 |
587-764 |
sentence |
denotes |
Adult mice were given an intraperitoneal injection of 30 mg/kg calcein at 7 days and 30 mg/kg tetracycline at 2 days prior to sacrifice to label actively mineralizing surfaces. |
T8976 |
588-593 |
JJ |
denotes |
Adult |
T8977 |
594-598 |
NNS |
denotes |
mice |
T8979 |
599-603 |
VBD |
denotes |
were |
T8978 |
604-609 |
VBN |
denotes |
given |
T8980 |
610-612 |
DT |
denotes |
an |
T8982 |
613-628 |
JJ |
denotes |
intraperitoneal |
T8981 |
629-638 |
NN |
denotes |
injection |
T8983 |
639-641 |
IN |
denotes |
of |
T8984 |
642-644 |
CD |
denotes |
30 |
T8985 |
645-647 |
NN |
denotes |
mg |
T8987 |
647-648 |
SYM |
denotes |
/ |
T8988 |
648-650 |
NN |
denotes |
kg |
T8986 |
651-658 |
NN |
denotes |
calcein |
T8989 |
659-661 |
IN |
denotes |
at |
T8990 |
662-663 |
CD |
denotes |
7 |
T8991 |
664-668 |
NNS |
denotes |
days |
T8992 |
669-672 |
CC |
denotes |
and |
T8993 |
673-675 |
CD |
denotes |
30 |
T8994 |
676-678 |
NN |
denotes |
mg |
T8996 |
678-679 |
SYM |
denotes |
/ |
T8997 |
679-681 |
NN |
denotes |
kg |
T8995 |
682-694 |
NN |
denotes |
tetracycline |
T8998 |
695-697 |
IN |
denotes |
at |
T8999 |
698-699 |
CD |
denotes |
2 |
T9000 |
700-704 |
NNS |
denotes |
days |
T9001 |
705-710 |
JJ |
denotes |
prior |
T9002 |
711-713 |
IN |
denotes |
to |
T9003 |
714-723 |
NN |
denotes |
sacrifice |
T9004 |
724-726 |
TO |
denotes |
to |
T9005 |
727-732 |
VB |
denotes |
label |
T9006 |
733-741 |
RB |
denotes |
actively |
T9007 |
742-754 |
VBG |
denotes |
mineralizing |
T9008 |
755-763 |
NNS |
denotes |
surfaces |
T9009 |
763-764 |
. |
denotes |
. |
T9010 |
764-961 |
sentence |
denotes |
After overnight fixation in 4% paraformaldehyde and rinsing in PBS, the left femur and tibia were embedded in polymethylmethacrylate (MMA) or a mixture of 50% MMA and 50% glycolmethacrylate (GMA). |
T9011 |
765-770 |
IN |
denotes |
After |
T9013 |
771-780 |
RB |
denotes |
overnight |
T9014 |
781-789 |
NN |
denotes |
fixation |
T9015 |
790-792 |
IN |
denotes |
in |
T9016 |
793-794 |
CD |
denotes |
4 |
T9017 |
794-795 |
NN |
denotes |
% |
T9018 |
796-812 |
NN |
denotes |
paraformaldehyde |
T9019 |
813-816 |
CC |
denotes |
and |
T9020 |
817-824 |
VBG |
denotes |
rinsing |
T9021 |
825-827 |
IN |
denotes |
in |
T9022 |
828-831 |
NN |
denotes |
PBS |
T9023 |
831-833 |
, |
denotes |
, |
T9024 |
833-836 |
DT |
denotes |
the |
T9026 |
837-841 |
JJ |
denotes |
left |
T9025 |
842-847 |
NN |
denotes |
femur |
T9027 |
848-851 |
CC |
denotes |
and |
T9028 |
852-857 |
NN |
denotes |
tibia |
T9029 |
858-862 |
VBD |
denotes |
were |
T9012 |
863-871 |
VBN |
denotes |
embedded |
T9030 |
872-874 |
IN |
denotes |
in |
T9031 |
875-897 |
NN |
denotes |
polymethylmethacrylate |
T9032 |
898-899 |
-LRB- |
denotes |
( |
T9033 |
899-902 |
NN |
denotes |
MMA |
T9034 |
902-903 |
-RRB- |
denotes |
) |
T9035 |
904-906 |
CC |
denotes |
or |
T9036 |
907-908 |
DT |
denotes |
a |
T9037 |
909-916 |
NN |
denotes |
mixture |
T9038 |
917-919 |
IN |
denotes |
of |
T9039 |
920-922 |
CD |
denotes |
50 |
T9040 |
922-923 |
NN |
denotes |
% |
T9041 |
924-927 |
NN |
denotes |
MMA |
T9042 |
928-931 |
CC |
denotes |
and |
T9043 |
932-934 |
CD |
denotes |
50 |
T9044 |
934-935 |
NN |
denotes |
% |
T9045 |
936-954 |
NN |
denotes |
glycolmethacrylate |
T9046 |
955-956 |
-LRB- |
denotes |
( |
T9047 |
956-959 |
NN |
denotes |
GMA |
T9048 |
959-960 |
-RRB- |
denotes |
) |
T9049 |
960-961 |
. |
denotes |
. |
T9050 |
961-1175 |
sentence |
denotes |
Serial 4- to 6-μm sections of MMA-embedded tissues were left unstained or stained with von Kossa and toluidine blue or with toluidine blue alone, while 4-μm MMA-GMA sections were stained for TRAP and ALP activity. |
T9051 |
962-968 |
JJ |
denotes |
Serial |
T9053 |
969-970 |
CD |
denotes |
4 |
T9055 |
970-971 |
HYPH |
denotes |
- |
T9056 |
972-974 |
IN |
denotes |
to |
T9054 |
975-976 |
CD |
denotes |
6 |
T9058 |
976-977 |
HYPH |
denotes |
- |
T9057 |
977-979 |
NN |
denotes |
μm |
T9052 |
980-988 |
NNS |
denotes |
sections |
T9060 |
989-991 |
IN |
denotes |
of |
T9061 |
992-995 |
NN |
denotes |
MMA |
T9063 |
995-996 |
HYPH |
denotes |
- |
T9062 |
996-1004 |
VBN |
denotes |
embedded |
T9064 |
1005-1012 |
NNS |
denotes |
tissues |
T9065 |
1013-1017 |
VBD |
denotes |
were |
T9059 |
1018-1022 |
VBN |
denotes |
left |
T9066 |
1023-1032 |
JJ |
denotes |
unstained |
T9067 |
1033-1035 |
CC |
denotes |
or |
T9068 |
1036-1043 |
VBN |
denotes |
stained |
T9069 |
1044-1048 |
IN |
denotes |
with |
T9070 |
1049-1052 |
NNP |
denotes |
von |
T9071 |
1053-1058 |
NNP |
denotes |
Kossa |
T9072 |
1059-1062 |
CC |
denotes |
and |
T9073 |
1063-1072 |
NN |
denotes |
toluidine |
T9074 |
1073-1077 |
NN |
denotes |
blue |
T9075 |
1078-1080 |
CC |
denotes |
or |
T9076 |
1081-1085 |
IN |
denotes |
with |
T9077 |
1086-1095 |
NN |
denotes |
toluidine |
T9078 |
1096-1100 |
NN |
denotes |
blue |
T9079 |
1101-1106 |
RB |
denotes |
alone |
T9080 |
1106-1108 |
, |
denotes |
, |
T9081 |
1108-1113 |
IN |
denotes |
while |
T9083 |
1114-1115 |
CD |
denotes |
4 |
T9085 |
1115-1116 |
HYPH |
denotes |
- |
T9084 |
1116-1118 |
NN |
denotes |
μm |
T9087 |
1119-1122 |
NN |
denotes |
MMA |
T9089 |
1122-1123 |
HYPH |
denotes |
- |
T9088 |
1123-1126 |
NN |
denotes |
GMA |
T9086 |
1127-1135 |
NNS |
denotes |
sections |
T9090 |
1136-1140 |
VBD |
denotes |
were |
T9082 |
1141-1148 |
VBN |
denotes |
stained |
T9091 |
1149-1152 |
IN |
denotes |
for |
T9092 |
1153-1157 |
NN |
denotes |
TRAP |
T9094 |
1158-1161 |
CC |
denotes |
and |
T9095 |
1162-1165 |
NN |
denotes |
ALP |
T9093 |
1166-1174 |
NN |
denotes |
activity |
T9096 |
1174-1175 |
. |
denotes |
. |
T9097 |
1175-1494 |
sentence |
denotes |
Images were captured using a Leica DMR microscope (Leica Microsystems) equipped with a Retiga 1300 camera (Qimaging, Burnaby, British Columbia, Canada) and the primary histomorphometric data obtained using Bioquant Nova Prime image analysis software (Bioquant Image Analysis Corp, Nashville, Tennessee, United States). |
T9098 |
1176-1182 |
NNS |
denotes |
Images |
T9100 |
1183-1187 |
VBD |
denotes |
were |
T9099 |
1188-1196 |
VBN |
denotes |
captured |
T9101 |
1197-1202 |
VBG |
denotes |
using |
T9102 |
1203-1204 |
DT |
denotes |
a |
T9104 |
1205-1210 |
NNP |
denotes |
Leica |
T9105 |
1211-1214 |
NN |
denotes |
DMR |
T9103 |
1215-1225 |
NN |
denotes |
microscope |
T9106 |
1226-1227 |
-LRB- |
denotes |
( |
T9108 |
1227-1232 |
NNP |
denotes |
Leica |
T9107 |
1233-1245 |
NNP |
denotes |
Microsystems |
T9109 |
1245-1246 |
-RRB- |
denotes |
) |
T9110 |
1247-1255 |
VBN |
denotes |
equipped |
T9111 |
1256-1260 |
IN |
denotes |
with |
T9112 |
1261-1262 |
DT |
denotes |
a |
T9114 |
1263-1269 |
NNP |
denotes |
Retiga |
T9115 |
1270-1274 |
CD |
denotes |
1300 |
T9113 |
1275-1281 |
NN |
denotes |
camera |
T9116 |
1282-1283 |
-LRB- |
denotes |
( |
T9117 |
1283-1291 |
NNP |
denotes |
Qimaging |
T9118 |
1291-1293 |
, |
denotes |
, |
T9119 |
1293-1300 |
NNP |
denotes |
Burnaby |
T9120 |
1300-1302 |
, |
denotes |
, |
T9121 |
1302-1309 |
NNP |
denotes |
British |
T9122 |
1310-1318 |
NNP |
denotes |
Columbia |
T9123 |
1318-1320 |
, |
denotes |
, |
T9124 |
1320-1326 |
NNP |
denotes |
Canada |
T9125 |
1326-1327 |
-RRB- |
denotes |
) |
T9126 |
1328-1331 |
CC |
denotes |
and |
T9127 |
1332-1335 |
DT |
denotes |
the |
T9129 |
1336-1343 |
JJ |
denotes |
primary |
T9130 |
1344-1361 |
JJ |
denotes |
histomorphometric |
T9128 |
1362-1366 |
NNS |
denotes |
data |
T9131 |
1367-1375 |
VBN |
denotes |
obtained |
T9132 |
1376-1381 |
VBG |
denotes |
using |
T9133 |
1382-1390 |
NNP |
denotes |
Bioquant |
T9135 |
1391-1395 |
NNP |
denotes |
Nova |
T9134 |
1396-1401 |
NNP |
denotes |
Prime |
T9137 |
1402-1407 |
NN |
denotes |
image |
T9138 |
1408-1416 |
NN |
denotes |
analysis |
T9136 |
1417-1425 |
NN |
denotes |
software |
T9139 |
1426-1427 |
-LRB- |
denotes |
( |
T9141 |
1427-1435 |
NNP |
denotes |
Bioquant |
T9142 |
1436-1441 |
NNP |
denotes |
Image |
T9143 |
1442-1450 |
NNP |
denotes |
Analysis |
T9140 |
1451-1455 |
NNP |
denotes |
Corp |
T9144 |
1455-1457 |
, |
denotes |
, |
T9145 |
1457-1466 |
NNP |
denotes |
Nashville |
T9146 |
1466-1468 |
, |
denotes |
, |
T9147 |
1468-1477 |
NNP |
denotes |
Tennessee |
T9148 |
1477-1479 |
, |
denotes |
, |
T9149 |
1479-1485 |
NNP |
denotes |
United |
T9150 |
1486-1492 |
NNP |
denotes |
States |
T9151 |
1492-1493 |
-RRB- |
denotes |
) |
T9152 |
1493-1494 |
. |
denotes |
. |
T9153 |
1494-1614 |
sentence |
denotes |
Nomenclature and abbreviations conform to those recommended by the American Society for Bone and Mineral Research [50]. |
T9154 |
1495-1507 |
NN |
denotes |
Nomenclature |
T9156 |
1508-1511 |
CC |
denotes |
and |
T9157 |
1512-1525 |
NNS |
denotes |
abbreviations |
T9155 |
1526-1533 |
VBP |
denotes |
conform |
T9158 |
1534-1536 |
IN |
denotes |
to |
T9159 |
1537-1542 |
DT |
denotes |
those |
T9160 |
1543-1554 |
VBN |
denotes |
recommended |
T9161 |
1555-1557 |
IN |
denotes |
by |
T9162 |
1558-1561 |
DT |
denotes |
the |
T9164 |
1562-1570 |
NNP |
denotes |
American |
T9163 |
1571-1578 |
NNP |
denotes |
Society |
T9165 |
1579-1582 |
IN |
denotes |
for |
T9166 |
1583-1587 |
NNP |
denotes |
Bone |
T9168 |
1588-1591 |
CC |
denotes |
and |
T9169 |
1592-1599 |
NNP |
denotes |
Mineral |
T9167 |
1600-1608 |
NNP |
denotes |
Research |
T9170 |
1609-1610 |
-LRB- |
denotes |
[ |
T9171 |
1610-1612 |
CD |
denotes |
50 |
T9172 |
1612-1613 |
-RRB- |
denotes |
] |
T9173 |
1613-1614 |
. |
denotes |
. |
R5474 |
T8863 |
T8864 |
amod |
Histologic,analyses |
R5475 |
T8865 |
T8863 |
punct |
", ",Histologic |
R5476 |
T8866 |
T8863 |
conj |
immunohistochemical,Histologic |
R5477 |
T8867 |
T8866 |
punct |
", ",immunohistochemical |
R5478 |
T8868 |
T8866 |
cc |
and,immunohistochemical |
R5479 |
T8869 |
T8866 |
conj |
histomorphometric,immunohistochemical |
R5480 |
T8870 |
T8864 |
punct |
.,analyses |
R5481 |
T8872 |
T8873 |
det |
All,analyses |
R5482 |
T8873 |
T8874 |
nsubjpass |
analyses,performed |
R5483 |
T8875 |
T8874 |
auxpass |
were,performed |
R5484 |
T8876 |
T8877 |
advmod |
essentially,described |
R5485 |
T8877 |
T8874 |
advcl |
described,performed |
R5486 |
T8878 |
T8877 |
mark |
as,described |
R5487 |
T8879 |
T8877 |
advmod |
previously,described |
R5488 |
T8880 |
T8881 |
punct |
[,63 |
R5489 |
T8881 |
T8874 |
parataxis |
63,performed |
R5490 |
T8882 |
T8881 |
nummod |
62,63 |
R5491 |
T8883 |
T8881 |
punct |
",",63 |
R5492 |
T8884 |
T8881 |
punct |
],63 |
R5493 |
T8885 |
T8874 |
punct |
.,performed |
R5494 |
T8887 |
T8888 |
advmod |
Briefly,removed |
R5495 |
T8889 |
T8888 |
punct |
", ",removed |
R5496 |
T8890 |
T8891 |
amod |
embryonic,mice |
R5497 |
T8891 |
T8888 |
nsubjpass |
mice,removed |
R5498 |
T8892 |
T8888 |
auxpass |
were,removed |
R5499 |
T8893 |
T8888 |
prep |
from,removed |
R5500 |
T8894 |
T8895 |
amod |
timed,dams |
R5501 |
T8895 |
T8893 |
pobj |
dams,from |
R5502 |
T8896 |
T8895 |
amod |
pregnant,dams |
R5503 |
T8897 |
T8888 |
prep |
at,removed |
R5504 |
T8898 |
T8897 |
pobj |
E14.5,at |
R5505 |
T8899 |
T8898 |
cc |
and,E14.5 |
R5506 |
T8900 |
T8898 |
conj |
E16.5,E14.5 |
R5507 |
T8901 |
T8888 |
cc |
and,removed |
R5508 |
T8902 |
T8888 |
conj |
fixed,removed |
R5509 |
T8903 |
T8902 |
advcl |
intact,fixed |
R5510 |
T8904 |
T8902 |
prep |
for,fixed |
R5511 |
T8905 |
T8906 |
nummod |
36,h |
R5512 |
T8906 |
T8904 |
pobj |
h,for |
R5513 |
T8907 |
T8902 |
prep |
in,fixed |
R5514 |
T8908 |
T8909 |
nummod |
4,% |
R5515 |
T8909 |
T8910 |
compound |
%,paraformaldehyde |
R5516 |
T8910 |
T8907 |
pobj |
paraformaldehyde,in |
R5517 |
T8911 |
T8902 |
punct |
", ",fixed |
R5518 |
T8912 |
T8902 |
conj |
rinsed,fixed |
R5519 |
T8913 |
T8912 |
advmod |
thoroughly,rinsed |
R5520 |
T8914 |
T8912 |
prep |
in,rinsed |
R5521 |
T8915 |
T8914 |
pobj |
PBS,in |
R5522 |
T8916 |
T8912 |
punct |
", ",rinsed |
R5523 |
T8917 |
T8912 |
cc |
and,rinsed |
R5524 |
T8918 |
T8912 |
conj |
processed,rinsed |
R5525 |
T8919 |
T8918 |
prep |
for,processed |
R5526 |
T8920 |
T8921 |
compound |
paraffin,embedding |
R5527 |
T8921 |
T8919 |
pobj |
embedding,for |
R5528 |
T8922 |
T8888 |
punct |
.,removed |
R5529 |
T8924 |
T8925 |
amod |
Serial,sections |
R5530 |
T8925 |
T8929 |
nsubjpass |
sections,cut |
R5531 |
T8926 |
T8927 |
nummod |
4,μm |
R5532 |
T8927 |
T8925 |
compound |
μm,sections |
R5533 |
T8928 |
T8927 |
punct |
-,μm |
R5534 |
T8930 |
T8929 |
auxpass |
were,cut |
R5535 |
T8931 |
T8929 |
prep |
on,cut |
R5536 |
T8932 |
T8933 |
det |
a,microtome |
R5537 |
T8933 |
T8931 |
pobj |
microtome,on |
R5538 |
T8934 |
T8933 |
amod |
modified,microtome |
R5539 |
T8935 |
T8936 |
nmod |
Leica,RM |
R5540 |
T8936 |
T8933 |
nmod |
RM,microtome |
R5541 |
T8937 |
T8936 |
nummod |
2155,RM |
R5542 |
T8938 |
T8933 |
amod |
rotary,microtome |
R5543 |
T8939 |
T8940 |
punct |
(,Microsystems |
R5544 |
T8940 |
T8933 |
parataxis |
Microsystems,microtome |
R5545 |
T8941 |
T8940 |
compound |
Leica,Microsystems |
R5546 |
T8942 |
T8940 |
punct |
", ",Microsystems |
R5547 |
T8943 |
T8944 |
compound |
Richmond,Hill |
R5548 |
T8944 |
T8940 |
npadvmod |
Hill,Microsystems |
R5549 |
T8945 |
T8940 |
punct |
", ",Microsystems |
R5550 |
T8946 |
T8940 |
npadvmod |
Ontario,Microsystems |
R5551 |
T8947 |
T8940 |
punct |
", ",Microsystems |
R5552 |
T8948 |
T8940 |
npadvmod |
Canada,Microsystems |
R5553 |
T8949 |
T8940 |
punct |
),Microsystems |
R5554 |
T8950 |
T8929 |
punct |
", ",cut |
R5555 |
T8951 |
T8929 |
conj |
stained,cut |
R5556 |
T8952 |
T8951 |
prep |
with,stained |
R5557 |
T8953 |
T8954 |
det |
a,dilution |
R5558 |
T8954 |
T8952 |
pobj |
dilution,with |
R5559 |
T8955 |
T8954 |
nummod |
1,dilution |
R5560 |
T8956 |
T8957 |
punct |
:,600 |
R5561 |
T8957 |
T8955 |
prep |
600,1 |
R5562 |
T8958 |
T8954 |
prep |
of,dilution |
R5563 |
T8959 |
T8960 |
det |
the,antibody |
R5564 |
T8960 |
T8958 |
pobj |
antibody,of |
R5565 |
T8961 |
T8960 |
nmod |
AD1,antibody |
R5566 |
T8962 |
T8960 |
amod |
anti-Sam68,antibody |
R5567 |
T8963 |
T8964 |
punct |
[,46 |
R5568 |
T8964 |
T8951 |
parataxis |
46,stained |
R5569 |
T8965 |
T8964 |
punct |
],46 |
R5570 |
T8966 |
T8951 |
cc |
and,stained |
R5571 |
T8967 |
T8951 |
conj |
counterstained,stained |
R5572 |
T8968 |
T8967 |
prep |
with,counterstained |
R5573 |
T8969 |
T8970 |
preconj |
either,green |
R5574 |
T8970 |
T8968 |
pobj |
green,with |
R5575 |
T8971 |
T8970 |
compound |
methyl,green |
R5576 |
T8972 |
T8970 |
cc |
or,green |
R5577 |
T8973 |
T8970 |
conj |
hematoxylin,green |
R5578 |
T8974 |
T8929 |
punct |
.,cut |
R5579 |
T8976 |
T8977 |
amod |
Adult,mice |
R5580 |
T8977 |
T8978 |
nsubj |
mice,given |
R5581 |
T8979 |
T8978 |
aux |
were,given |
R5582 |
T8980 |
T8981 |
det |
an,injection |
R5583 |
T8981 |
T8978 |
dobj |
injection,given |
R5584 |
T8982 |
T8981 |
amod |
intraperitoneal,injection |
R5585 |
T8983 |
T8981 |
prep |
of,injection |
R5586 |
T8984 |
T8985 |
nummod |
30,mg |
R5587 |
T8985 |
T8986 |
nmod |
mg,calcein |
R5588 |
T8986 |
T8983 |
pobj |
calcein,of |
R5589 |
T8987 |
T8988 |
punct |
/,kg |
R5590 |
T8988 |
T8985 |
prep |
kg,mg |
R5591 |
T8989 |
T8978 |
prep |
at,given |
R5592 |
T8990 |
T8991 |
nummod |
7,days |
R5593 |
T8991 |
T8989 |
pobj |
days,at |
R5594 |
T8992 |
T8978 |
cc |
and,given |
R5595 |
T8993 |
T8994 |
nummod |
30,mg |
R5596 |
T8994 |
T8995 |
nmod |
mg,tetracycline |
R5597 |
T8995 |
T8978 |
conj |
tetracycline,given |
R5598 |
T8996 |
T8997 |
punct |
/,kg |
R5599 |
T8997 |
T8994 |
prep |
kg,mg |
R5600 |
T8998 |
T8995 |
prep |
at,tetracycline |
R5601 |
T8999 |
T9000 |
nummod |
2,days |
R5602 |
T9000 |
T8998 |
pobj |
days,at |
R5603 |
T9001 |
T9002 |
amod |
prior,to |
R5604 |
T9002 |
T9000 |
prep |
to,days |
R5605 |
T9003 |
T9002 |
pobj |
sacrifice,to |
R5606 |
T9004 |
T9005 |
aux |
to,label |
R5607 |
T9005 |
T8978 |
advcl |
label,given |
R5608 |
T9006 |
T9007 |
advmod |
actively,mineralizing |
R5609 |
T9007 |
T9008 |
amod |
mineralizing,surfaces |
R5610 |
T9008 |
T9005 |
dobj |
surfaces,label |
R5611 |
T9009 |
T8978 |
punct |
.,given |
R5612 |
T9011 |
T9012 |
prep |
After,embedded |
R5613 |
T9013 |
T9014 |
advmod |
overnight,fixation |
R5614 |
T9014 |
T9011 |
pobj |
fixation,After |
R5615 |
T9015 |
T9014 |
prep |
in,fixation |
R5616 |
T9016 |
T9017 |
nummod |
4,% |
R5617 |
T9017 |
T9018 |
compound |
%,paraformaldehyde |
R5618 |
T9018 |
T9015 |
pobj |
paraformaldehyde,in |
R5619 |
T9019 |
T9014 |
cc |
and,fixation |
R5620 |
T9020 |
T9014 |
conj |
rinsing,fixation |
R5621 |
T9021 |
T9020 |
prep |
in,rinsing |
R5622 |
T9022 |
T9021 |
pobj |
PBS,in |
R5623 |
T9023 |
T9012 |
punct |
", ",embedded |
R5624 |
T9024 |
T9025 |
det |
the,femur |
R5625 |
T9025 |
T9012 |
nsubjpass |
femur,embedded |
R5626 |
T9026 |
T9025 |
amod |
left,femur |
R5627 |
T9027 |
T9025 |
cc |
and,femur |
R5628 |
T9028 |
T9025 |
conj |
tibia,femur |
R5629 |
T9029 |
T9012 |
auxpass |
were,embedded |
R5630 |
T9030 |
T9012 |
prep |
in,embedded |
R5631 |
T9031 |
T9030 |
pobj |
polymethylmethacrylate,in |
R5632 |
T9032 |
T9031 |
punct |
(,polymethylmethacrylate |
R5633 |
T9033 |
T9031 |
appos |
MMA,polymethylmethacrylate |
R5634 |
T9034 |
T9031 |
punct |
),polymethylmethacrylate |
R5635 |
T9035 |
T9031 |
cc |
or,polymethylmethacrylate |
R5636 |
T9036 |
T9037 |
det |
a,mixture |
R5637 |
T9037 |
T9031 |
conj |
mixture,polymethylmethacrylate |
R5638 |
T9038 |
T9037 |
prep |
of,mixture |
R5639 |
T9039 |
T9040 |
nummod |
50,% |
R5640 |
T9040 |
T9041 |
compound |
%,MMA |
R5641 |
T9041 |
T9038 |
pobj |
MMA,of |
R5642 |
T9042 |
T9041 |
cc |
and,MMA |
R5643 |
T9043 |
T9044 |
nummod |
50,% |
R5644 |
T9044 |
T9041 |
conj |
%,MMA |
R5645 |
T9045 |
T9044 |
appos |
glycolmethacrylate,% |
R5646 |
T9046 |
T9045 |
punct |
(,glycolmethacrylate |
R5647 |
T9047 |
T9045 |
appos |
GMA,glycolmethacrylate |
R5648 |
T9048 |
T9037 |
punct |
),mixture |
R5649 |
T9049 |
T9012 |
punct |
.,embedded |
R5650 |
T9051 |
T9052 |
amod |
Serial,sections |
R5651 |
T9052 |
T9059 |
nsubjpass |
sections,left |
R5652 |
T9053 |
T9054 |
quantmod |
4,6 |
R5653 |
T9054 |
T9057 |
nummod |
6,μm |
R5654 |
T9055 |
T9054 |
punct |
-,6 |
R5655 |
T9056 |
T9054 |
quantmod |
to,6 |
R5656 |
T9057 |
T9052 |
compound |
μm,sections |
R5657 |
T9058 |
T9057 |
punct |
-,μm |
R5658 |
T9060 |
T9052 |
prep |
of,sections |
R5659 |
T9061 |
T9062 |
npadvmod |
MMA,embedded |
R5660 |
T9062 |
T9064 |
amod |
embedded,tissues |
R5661 |
T9063 |
T9062 |
punct |
-,embedded |
R5662 |
T9064 |
T9060 |
pobj |
tissues,of |
R5663 |
T9065 |
T9059 |
auxpass |
were,left |
R5664 |
T9066 |
T9059 |
oprd |
unstained,left |
R5665 |
T9067 |
T9059 |
cc |
or,left |
R5666 |
T9068 |
T9059 |
conj |
stained,left |
R5667 |
T9069 |
T9068 |
prep |
with,stained |
R5668 |
T9070 |
T9071 |
compound |
von,Kossa |
R5669 |
T9071 |
T9069 |
pobj |
Kossa,with |
R5670 |
T9072 |
T9071 |
cc |
and,Kossa |
R5671 |
T9073 |
T9074 |
compound |
toluidine,blue |
R5672 |
T9074 |
T9071 |
conj |
blue,Kossa |
R5673 |
T9075 |
T9069 |
cc |
or,with |
R5674 |
T9076 |
T9069 |
conj |
with,with |
R5675 |
T9077 |
T9078 |
compound |
toluidine,blue |
R5676 |
T9078 |
T9076 |
pobj |
blue,with |
R5677 |
T9079 |
T9078 |
advmod |
alone,blue |
R5678 |
T9080 |
T9059 |
punct |
", ",left |
R5679 |
T9081 |
T9082 |
mark |
while,stained |
R5680 |
T9082 |
T9059 |
advcl |
stained,left |
R5681 |
T9083 |
T9084 |
nummod |
4,μm |
R5682 |
T9084 |
T9086 |
compound |
μm,sections |
R5683 |
T9085 |
T9084 |
punct |
-,μm |
R5684 |
T9086 |
T9082 |
nsubjpass |
sections,stained |
R5685 |
T9087 |
T9088 |
compound |
MMA,GMA |
R5686 |
T9088 |
T9086 |
compound |
GMA,sections |
R5687 |
T9089 |
T9088 |
punct |
-,GMA |
R5688 |
T9090 |
T9082 |
auxpass |
were,stained |
R5689 |
T9091 |
T9082 |
prep |
for,stained |
R5690 |
T9092 |
T9093 |
nmod |
TRAP,activity |
R5691 |
T9093 |
T9091 |
pobj |
activity,for |
R5692 |
T9094 |
T9092 |
cc |
and,TRAP |
R5693 |
T9095 |
T9092 |
conj |
ALP,TRAP |
R5694 |
T9096 |
T9059 |
punct |
.,left |
R5695 |
T9098 |
T9099 |
nsubjpass |
Images,captured |
R5696 |
T9100 |
T9099 |
auxpass |
were,captured |
R5697 |
T9101 |
T9099 |
advcl |
using,captured |
R5698 |
T9102 |
T9103 |
det |
a,microscope |
R5699 |
T9103 |
T9101 |
dobj |
microscope,using |
R5700 |
T9104 |
T9105 |
compound |
Leica,DMR |
R5701 |
T9105 |
T9103 |
compound |
DMR,microscope |
R5702 |
T9106 |
T9107 |
punct |
(,Microsystems |
R5703 |
T9107 |
T9103 |
parataxis |
Microsystems,microscope |
R5704 |
T9108 |
T9107 |
compound |
Leica,Microsystems |
R5705 |
T9109 |
T9107 |
punct |
),Microsystems |
R5706 |
T9110 |
T9103 |
acl |
equipped,microscope |
R5707 |
T9111 |
T9110 |
prep |
with,equipped |
R5708 |
T9112 |
T9113 |
det |
a,camera |
R5709 |
T9113 |
T9111 |
pobj |
camera,with |
R5710 |
T9114 |
T9113 |
nmod |
Retiga,camera |
R5711 |
T9115 |
T9114 |
nummod |
1300,Retiga |
R5712 |
T9116 |
T9117 |
punct |
(,Qimaging |
R5713 |
T9117 |
T9113 |
parataxis |
Qimaging,camera |
R5714 |
T9118 |
T9117 |
punct |
", ",Qimaging |
R5715 |
T9119 |
T9117 |
npadvmod |
Burnaby,Qimaging |
R5716 |
T9120 |
T9117 |
punct |
", ",Qimaging |
R5717 |
T9121 |
T9122 |
compound |
British,Columbia |
R5718 |
T9122 |
T9117 |
npadvmod |
Columbia,Qimaging |
R5719 |
T9123 |
T9117 |
punct |
", ",Qimaging |
R5720 |
T9124 |
T9117 |
npadvmod |
Canada,Qimaging |
R5721 |
T9125 |
T9117 |
punct |
),Qimaging |
R5722 |
T9126 |
T9099 |
cc |
and,captured |
R5723 |
T9127 |
T9128 |
det |
the,data |
R5724 |
T9128 |
T9131 |
nsubj |
data,obtained |
R5725 |
T9129 |
T9128 |
amod |
primary,data |
R5726 |
T9130 |
T9128 |
amod |
histomorphometric,data |
R5727 |
T9131 |
T9099 |
conj |
obtained,captured |
R5728 |
T9132 |
T9131 |
advcl |
using,obtained |
R5729 |
T9133 |
T9134 |
compound |
Bioquant,Prime |
R5730 |
T9134 |
T9136 |
compound |
Prime,software |
R5731 |
T9135 |
T9134 |
compound |
Nova,Prime |
R5732 |
T9136 |
T9132 |
dobj |
software,using |
R5733 |
T9137 |
T9138 |
compound |
image,analysis |
R5734 |
T9138 |
T9136 |
compound |
analysis,software |
R5735 |
T9139 |
T9140 |
punct |
(,Corp |
R5736 |
T9140 |
T9136 |
parataxis |
Corp,software |
R5737 |
T9141 |
T9140 |
compound |
Bioquant,Corp |
R5738 |
T9142 |
T9143 |
compound |
Image,Analysis |
R5739 |
T9143 |
T9140 |
compound |
Analysis,Corp |
R5740 |
T9144 |
T9140 |
punct |
", ",Corp |
R5741 |
T9145 |
T9140 |
npadvmod |
Nashville,Corp |
R5742 |
T9146 |
T9140 |
punct |
", ",Corp |
R5743 |
T9147 |
T9140 |
npadvmod |
Tennessee,Corp |
R5744 |
T9148 |
T9140 |
punct |
", ",Corp |
R5745 |
T9149 |
T9150 |
compound |
United,States |
R5746 |
T9150 |
T9140 |
npadvmod |
States,Corp |
R5747 |
T9151 |
T9140 |
punct |
),Corp |
R5748 |
T9152 |
T9131 |
punct |
.,obtained |
R5749 |
T9154 |
T9155 |
nsubj |
Nomenclature,conform |
R5750 |
T9156 |
T9154 |
cc |
and,Nomenclature |
R5751 |
T9157 |
T9154 |
conj |
abbreviations,Nomenclature |
R5752 |
T9158 |
T9155 |
prep |
to,conform |
R5753 |
T9159 |
T9158 |
pobj |
those,to |
R5754 |
T9160 |
T9159 |
acl |
recommended,those |
R5755 |
T9161 |
T9160 |
agent |
by,recommended |
R5756 |
T9162 |
T9163 |
det |
the,Society |
R5757 |
T9163 |
T9161 |
pobj |
Society,by |
R5758 |
T9164 |
T9163 |
compound |
American,Society |
R5759 |
T9165 |
T9163 |
prep |
for,Society |
R5760 |
T9166 |
T9167 |
nmod |
Bone,Research |
R5761 |
T9167 |
T9165 |
pobj |
Research,for |
R5762 |
T9168 |
T9166 |
cc |
and,Bone |
R5763 |
T9169 |
T9166 |
conj |
Mineral,Bone |
R5764 |
T9170 |
T9171 |
punct |
[,50 |
R5765 |
T9171 |
T9155 |
parataxis |
50,conform |
R5766 |
T9172 |
T9171 |
punct |
],50 |
R5767 |
T9173 |
T9155 |
punct |
.,conform |