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PMC:1283364 / 9510-11110 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T2635 0-10 NN denotes Genotyping
T2636 10-141 sentence denotes Offspring were genotyped for the presence of each transgene by PCR amplification of genomic DNA extracted from a 5-mm tail biopsy.
T2637 11-20 NN denotes Offspring
T2639 21-25 VBD denotes were
T2638 26-35 VBN denotes genotyped
T2640 36-39 IN denotes for
T2641 40-43 DT denotes the
T2642 44-52 NN denotes presence
T2643 53-55 IN denotes of
T2644 56-60 DT denotes each
T2645 61-70 NN denotes transgene
T2646 71-73 IN denotes by
T2647 74-77 NN denotes PCR
T2648 78-91 NN denotes amplification
T2649 92-94 IN denotes of
T2650 95-102 JJ denotes genomic
T2651 103-106 NN denotes DNA
T2652 107-116 VBN denotes extracted
T2653 117-121 IN denotes from
T2654 122-123 DT denotes a
T2656 124-125 CD denotes 5
T2658 125-126 HYPH denotes -
T2657 126-128 NN denotes mm
T2659 129-133 NN denotes tail
T2655 134-140 NN denotes biopsy
T2660 140-141 . denotes .
T2661 141-280 sentence denotes Tails were heated to 95 °C for 45 min in 250 μl of 50 mM NaOH, vortexed, then neutralized with an equal volume of 0.5 M Tris-HCl (pH 5.5).
T2662 142-147 NNS denotes Tails
T2664 148-152 VBD denotes were
T2663 153-159 VBN denotes heated
T2665 160-162 IN denotes to
T2666 163-165 CD denotes 95
T2667 166-168 NN denotes °C
T2668 169-172 IN denotes for
T2669 173-175 CD denotes 45
T2670 176-179 NN denotes min
T2671 180-182 IN denotes in
T2672 183-186 CD denotes 250
T2673 187-189 NN denotes μl
T2674 190-192 IN denotes of
T2675 193-195 CD denotes 50
T2676 196-198 NN denotes mM
T2677 199-203 NN denotes NaOH
T2678 203-205 , denotes ,
T2679 205-213 VBN denotes vortexed
T2680 213-215 , denotes ,
T2681 215-219 RB denotes then
T2682 220-231 VBN denotes neutralized
T2683 232-236 IN denotes with
T2684 237-239 DT denotes an
T2686 240-245 JJ denotes equal
T2685 246-252 NN denotes volume
T2687 253-255 IN denotes of
T2688 256-259 CD denotes 0.5
T2689 260-261 NN denotes M
T2691 262-266 NN denotes Tris
T2692 266-267 HYPH denotes -
T2690 267-270 NN denotes HCl
T2693 271-272 -LRB- denotes (
T2694 272-274 NN denotes pH
T2695 275-278 CD denotes 5.5
T2696 278-279 -RRB- denotes )
T2697 279-280 . denotes .
T2698 280-373 sentence denotes Debris was sedimented by centrifugation, and 3 μl of supernatant was used for amplification.
T2699 281-287 NN denotes Debris
T2701 288-291 VBD denotes was
T2700 292-302 VBN denotes sedimented
T2702 303-305 IN denotes by
T2703 306-320 NN denotes centrifugation
T2704 320-322 , denotes ,
T2705 322-325 CC denotes and
T2706 326-327 CD denotes 3
T2707 328-330 NN denotes μl
T2709 331-333 IN denotes of
T2710 334-345 NN denotes supernatant
T2711 346-349 VBD denotes was
T2708 350-354 VBN denotes used
T2712 355-358 IN denotes for
T2713 359-372 NN denotes amplification
T2714 372-373 . denotes .
T2715 373-480 sentence denotes Genotyping for APP and tTA transgenes was performed in the same PCR reaction, using five separate primers.
T2716 374-384 VBG denotes Genotyping
T2718 385-388 IN denotes for
T2719 389-392 NN denotes APP
T2721 393-396 CC denotes and
T2722 397-400 NN denotes tTA
T2720 401-411 NNS denotes transgenes
T2723 412-415 VBD denotes was
T2717 416-425 VBN denotes performed
T2724 426-428 IN denotes in
T2725 429-432 DT denotes the
T2727 433-437 JJ denotes same
T2728 438-441 NN denotes PCR
T2726 442-450 NN denotes reaction
T2729 450-452 , denotes ,
T2730 452-457 VBG denotes using
T2731 458-462 CD denotes five
T2733 463-471 JJ denotes separate
T2732 472-479 NNS denotes primers
T2734 479-480 . denotes .
T2735 480-697 sentence denotes APP was amplified using forward primer S36 located in the 3′ end of the APP cDNA (CCG AGA TCT CTG AAG TGA AGA TGG ATG) and reverse primer PrP-AS-J located in the 3′ UTR of the vector (CCA AGC CTA GAC CAC GAG AAT GC).
T2736 481-484 NN denotes APP
T2738 485-488 VBD denotes was
T2737 489-498 VBN denotes amplified
T2739 499-504 VBG denotes using
T2740 505-512 RB denotes forward
T2742 513-519 NN denotes primer
T2741 520-523 NN denotes S36
T2743 524-531 VBN denotes located
T2744 532-534 IN denotes in
T2745 535-538 DT denotes the
T2747 539-540 CD denotes 3
T2748 540-541 SYM denotes ′
T2746 542-545 NN denotes end
T2749 546-548 IN denotes of
T2750 549-552 DT denotes the
T2752 553-556 NN denotes APP
T2751 557-561 NN denotes cDNA
T2753 562-563 -LRB- denotes (
T2755 563-566 NN denotes CCG
T2756 567-570 NN denotes AGA
T2757 571-574 NN denotes TCT
T2758 575-578 NN denotes CTG
T2759 579-582 NN denotes AAG
T2760 583-586 NN denotes TGA
T2761 587-590 NN denotes AGA
T2762 591-594 NN denotes TGG
T2754 595-598 NN denotes ATG
T2763 598-599 -RRB- denotes )
T2764 600-603 CC denotes and
T2765 604-611 JJ denotes reverse
T2767 612-618 NN denotes primer
T2768 619-622 NN denotes PrP
T2769 622-623 HYPH denotes -
T2770 623-625 NN denotes AS
T2771 625-626 HYPH denotes -
T2766 626-627 NN denotes J
T2772 628-635 VBN denotes located
T2773 636-638 IN denotes in
T2774 639-642 DT denotes the
T2776 643-644 CD denotes 3
T2777 644-645 SYM denotes ′
T2775 646-649 NN denotes UTR
T2778 650-652 IN denotes of
T2779 653-656 DT denotes the
T2780 657-663 NN denotes vector
T2781 664-665 -LRB- denotes (
T2783 665-668 NN denotes CCA
T2784 669-672 NN denotes AGC
T2785 673-676 NN denotes CTA
T2786 677-680 NN denotes GAC
T2787 681-684 NN denotes CAC
T2788 685-688 NN denotes GAG
T2789 689-692 NN denotes AAT
T2782 693-695 NN denotes GC
T2790 695-696 -RRB- denotes )
T2791 696-697 . denotes .
T2792 697-953 sentence denotes The tTA transgene was detected using a primer set that amplified across its two subdomains with tet forward located within the Tn10 tetracycline repressor (CGC TGT GGG GCA TTT TAC TTT AG) and tet reverse within the HSV1 VP16 (CAT GTC CAG ATC GAA ATC GTC).
T2793 698-701 DT denotes The
T2795 702-705 NN denotes tTA
T2794 706-715 NN denotes transgene
T2797 716-719 VBD denotes was
T2796 720-728 VBN denotes detected
T2798 729-734 VBG denotes using
T2799 735-736 DT denotes a
T2801 737-743 NN denotes primer
T2800 744-747 NN denotes set
T2802 748-752 WDT denotes that
T2803 753-762 VBD denotes amplified
T2804 763-769 IN denotes across
T2805 770-773 PRP$ denotes its
T2807 774-777 CD denotes two
T2806 778-788 NNS denotes subdomains
T2808 789-793 IN denotes with
T2809 794-797 NN denotes tet
T2810 798-805 RB denotes forward
T2811 806-813 VBN denotes located
T2812 814-820 IN denotes within
T2813 821-824 DT denotes the
T2815 825-829 NN denotes Tn10
T2816 830-842 NN denotes tetracycline
T2814 843-852 NN denotes repressor
T2817 853-854 -LRB- denotes (
T2819 854-857 NN denotes CGC
T2820 858-861 NN denotes TGT
T2821 862-865 NN denotes GGG
T2822 866-869 NN denotes GCA
T2823 870-873 NN denotes TTT
T2824 874-877 NN denotes TAC
T2825 878-881 NN denotes TTT
T2818 882-884 NN denotes AG
T2826 884-885 -RRB- denotes )
T2827 886-889 CC denotes and
T2828 890-893 NN denotes tet
T2829 894-901 JJ denotes reverse
T2830 902-908 IN denotes within
T2831 909-912 DT denotes the
T2833 913-917 NN denotes HSV1
T2832 918-922 NN denotes VP16
T2834 923-924 -LRB- denotes (
T2836 924-927 NN denotes CAT
T2837 928-931 NN denotes GTC
T2838 932-935 NN denotes CAG
T2839 936-939 NN denotes ATC
T2840 940-943 NN denotes GAA
T2841 944-947 NN denotes ATC
T2835 948-951 NN denotes GTC
T2842 951-952 -RRB- denotes )
T2843 952-953 . denotes .
T2844 953-1306 sentence denotes All reactions, whether transgene-positive or not, amplified a segment of the endogenous prion protein gene as a control for DNA quality using a forward primer, PrP-S-J, specific to the mouse PrP open reading frame (GGG ACT ATG TGG ACT GAT GTC GG) and a reverse primer, PrP-AS-J, shared by the 3′ UTR of the endogenous PrP gene and the transgene vector.
T2845 954-957 DT denotes All
T2846 958-967 NNS denotes reactions
T2848 967-969 , denotes ,
T2849 969-976 IN denotes whether
T2851 977-986 NN denotes transgene
T2852 986-987 HYPH denotes -
T2850 987-995 JJ denotes positive
T2853 996-998 CC denotes or
T2854 999-1002 RB denotes not
T2855 1002-1004 , denotes ,
T2847 1004-1013 VBD denotes amplified
T2856 1014-1015 DT denotes a
T2857 1016-1023 NN denotes segment
T2858 1024-1026 IN denotes of
T2859 1027-1030 DT denotes the
T2861 1031-1041 JJ denotes endogenous
T2862 1042-1047 NN denotes prion
T2863 1048-1055 NN denotes protein
T2860 1056-1060 NN denotes gene
T2864 1061-1063 IN denotes as
T2865 1064-1065 DT denotes a
T2866 1066-1073 NN denotes control
T2867 1074-1077 IN denotes for
T2868 1078-1081 NN denotes DNA
T2869 1082-1089 NN denotes quality
T2870 1090-1095 VBG denotes using
T2871 1096-1097 DT denotes a
T2873 1098-1105 JJ denotes forward
T2872 1106-1112 NN denotes primer
T2874 1112-1114 , denotes ,
T2875 1114-1117 NN denotes PrP
T2877 1117-1118 HYPH denotes -
T2878 1118-1119 NN denotes S
T2879 1119-1120 HYPH denotes -
T2876 1120-1121 NN denotes J
T2880 1121-1123 , denotes ,
T2881 1123-1131 JJ denotes specific
T2882 1132-1134 IN denotes to
T2883 1135-1138 DT denotes the
T2885 1139-1144 NN denotes mouse
T2886 1145-1148 NN denotes PrP
T2887 1149-1153 JJ denotes open
T2888 1154-1161 NN denotes reading
T2884 1162-1167 NN denotes frame
T2889 1168-1169 -LRB- denotes (
T2891 1169-1172 NN denotes GGG
T2892 1173-1176 NN denotes ACT
T2893 1177-1180 NN denotes ATG
T2894 1181-1184 NN denotes TGG
T2895 1185-1188 NN denotes ACT
T2896 1189-1192 NN denotes GAT
T2897 1193-1196 NN denotes GTC
T2890 1197-1199 NN denotes GG
T2898 1199-1200 -RRB- denotes )
T2899 1201-1204 CC denotes and
T2900 1205-1206 DT denotes a
T2902 1207-1214 JJ denotes reverse
T2901 1215-1221 NN denotes primer
T2903 1221-1223 , denotes ,
T2904 1223-1226 NN denotes PrP
T2906 1226-1227 HYPH denotes -
T2907 1227-1229 NN denotes AS
T2908 1229-1230 HYPH denotes -
T2905 1230-1231 NN denotes J
T2909 1231-1233 , denotes ,
T2910 1233-1239 VBN denotes shared
T2911 1240-1242 IN denotes by
T2912 1243-1246 DT denotes the
T2914 1247-1248 CD denotes 3
T2915 1248-1249 SYM denotes ′
T2913 1250-1253 NN denotes UTR
T2916 1254-1256 IN denotes of
T2917 1257-1260 DT denotes the
T2919 1261-1271 JJ denotes endogenous
T2920 1272-1275 NN denotes PrP
T2918 1276-1280 NN denotes gene
T2921 1281-1284 CC denotes and
T2922 1285-1288 DT denotes the
T2924 1289-1298 NN denotes transgene
T2923 1299-1305 NN denotes vector
T2925 1305-1306 . denotes .
T2926 1306-1410 sentence denotes Amplification reactions were run for 37 cycles at 94 °C for 30 s, 64 °C for 1 min, and 72 °C for 1 min.
T2927 1307-1320 NN denotes Amplification
T2928 1321-1330 NNS denotes reactions
T2930 1331-1335 VBD denotes were
T2929 1336-1339 VBN denotes run
T2931 1340-1343 IN denotes for
T2932 1344-1346 CD denotes 37
T2933 1347-1353 NNS denotes cycles
T2934 1354-1356 IN denotes at
T2935 1357-1359 CD denotes 94
T2936 1360-1362 NN denotes °C
T2937 1363-1366 IN denotes for
T2938 1367-1369 CD denotes 30
T2939 1370-1371 NN denotes s
T2940 1371-1373 , denotes ,
T2941 1373-1375 CD denotes 64
T2942 1376-1378 NN denotes °C
T2943 1379-1382 IN denotes for
T2944 1383-1384 CD denotes 1
T2945 1385-1388 NN denotes min
T2946 1388-1390 , denotes ,
T2947 1390-1393 CC denotes and
T2948 1394-1396 CD denotes 72
T2949 1397-1399 NN denotes °C
T2950 1400-1403 IN denotes for
T2951 1404-1405 CD denotes 1
T2952 1406-1409 NN denotes min
T2953 1409-1410 . denotes .
T2954 1410-1501 sentence denotes All samples, transgenic and wild-type, gave a 750-bp product from the endogenous PrP gene.
T2955 1411-1414 DT denotes All
T2956 1415-1422 NNS denotes samples
T2958 1422-1424 , denotes ,
T2959 1424-1434 JJ denotes transgenic
T2960 1435-1438 CC denotes and
T2961 1439-1443 JJ denotes wild
T2963 1443-1444 HYPH denotes -
T2962 1444-1448 NN denotes type
T2964 1448-1450 , denotes ,
T2957 1450-1454 VBD denotes gave
T2965 1455-1456 DT denotes a
T2967 1457-1460 CD denotes 750
T2969 1460-1461 HYPH denotes -
T2968 1461-1463 NN denotes bp
T2966 1464-1471 NN denotes product
T2970 1472-1476 IN denotes from
T2971 1477-1480 DT denotes the
T2973 1481-1491 JJ denotes endogenous
T2974 1492-1495 NN denotes PrP
T2972 1496-1500 NN denotes gene
T2975 1500-1501 . denotes .
T2976 1501-1600 sentence denotes The APP transgene yielded an additional band at 400 bp; the tTA product fell in between at 480 bp.
T2977 1502-1505 DT denotes The
T2979 1506-1509 NN denotes APP
T2978 1510-1519 NN denotes transgene
T2980 1520-1527 VBD denotes yielded
T2982 1528-1530 DT denotes an
T2984 1531-1541 JJ denotes additional
T2983 1542-1546 NN denotes band
T2985 1547-1549 IN denotes at
T2986 1550-1553 CD denotes 400
T2987 1554-1556 NN denotes bp
T2988 1556-1557 : denotes ;
T2989 1558-1561 DT denotes the
T2991 1562-1565 NN denotes tTA
T2990 1566-1573 NN denotes product
T2981 1574-1578 VBD denotes fell
T2992 1579-1581 RB denotes in
T2993 1582-1589 RB denotes between
T2994 1590-1592 IN denotes at
T2995 1593-1596 CD denotes 480
T2996 1597-1599 NN denotes bp
T2997 1599-1600 . denotes .
R1718 T2637 T2638 nsubjpass Offspring,genotyped
R1719 T2639 T2638 auxpass were,genotyped
R1720 T2640 T2638 prep for,genotyped
R1721 T2641 T2642 det the,presence
R1722 T2642 T2640 pobj presence,for
R1723 T2643 T2642 prep of,presence
R1724 T2644 T2645 det each,transgene
R1725 T2645 T2643 pobj transgene,of
R1726 T2646 T2638 prep by,genotyped
R1727 T2647 T2648 compound PCR,amplification
R1728 T2648 T2646 pobj amplification,by
R1729 T2649 T2648 prep of,amplification
R1730 T2650 T2651 amod genomic,DNA
R1731 T2651 T2649 pobj DNA,of
R1732 T2652 T2651 acl extracted,DNA
R1733 T2653 T2652 prep from,extracted
R1734 T2654 T2655 det a,biopsy
R1735 T2655 T2653 pobj biopsy,from
R1736 T2656 T2657 nummod 5,mm
R1737 T2657 T2655 compound mm,biopsy
R1738 T2658 T2657 punct -,mm
R1739 T2659 T2655 compound tail,biopsy
R1740 T2660 T2638 punct .,genotyped
R1741 T2662 T2663 nsubjpass Tails,heated
R1742 T2664 T2663 auxpass were,heated
R1743 T2665 T2663 prep to,heated
R1744 T2666 T2667 nummod 95,°C
R1745 T2667 T2665 pobj °C,to
R1746 T2668 T2663 prep for,heated
R1747 T2669 T2670 nummod 45,min
R1748 T2670 T2668 pobj min,for
R1749 T2671 T2663 prep in,heated
R1750 T2672 T2673 nummod 250,μl
R1751 T2673 T2671 pobj μl,in
R1752 T2674 T2673 prep of,μl
R1753 T2675 T2676 nummod 50,mM
R1754 T2676 T2677 compound mM,NaOH
R1755 T2677 T2674 pobj NaOH,of
R1756 T2678 T2663 punct ", ",heated
R1757 T2679 T2663 dep vortexed,heated
R1758 T2680 T2663 punct ", ",heated
R1759 T2681 T2682 advmod then,neutralized
R1760 T2682 T2663 dep neutralized,heated
R1761 T2683 T2682 prep with,neutralized
R1762 T2684 T2685 det an,volume
R1763 T2685 T2683 pobj volume,with
R1764 T2686 T2685 amod equal,volume
R1765 T2687 T2685 prep of,volume
R1766 T2688 T2689 nummod 0.5,M
R1767 T2689 T2690 compound M,HCl
R1768 T2690 T2687 pobj HCl,of
R1769 T2691 T2690 compound Tris,HCl
R1770 T2692 T2690 punct -,HCl
R1771 T2693 T2694 punct (,pH
R1772 T2694 T2690 parataxis pH,HCl
R1773 T2695 T2694 nummod 5.5,pH
R1774 T2696 T2694 punct ),pH
R1775 T2697 T2663 punct .,heated
R1776 T2699 T2700 nsubjpass Debris,sedimented
R1777 T2701 T2700 auxpass was,sedimented
R1778 T2702 T2700 prep by,sedimented
R1779 T2703 T2702 pobj centrifugation,by
R1780 T2704 T2700 punct ", ",sedimented
R1781 T2705 T2700 cc and,sedimented
R1782 T2706 T2707 nummod 3,μl
R1783 T2707 T2708 nsubjpass μl,used
R1784 T2708 T2700 conj used,sedimented
R1785 T2709 T2707 prep of,μl
R1786 T2710 T2709 pobj supernatant,of
R1787 T2711 T2708 auxpass was,used
R1788 T2712 T2708 prep for,used
R1789 T2713 T2712 pobj amplification,for
R1790 T2714 T2708 punct .,used
R1791 T2716 T2717 nsubjpass Genotyping,performed
R1792 T2718 T2716 prep for,Genotyping
R1793 T2719 T2720 nmod APP,transgenes
R1794 T2720 T2718 pobj transgenes,for
R1795 T2721 T2719 cc and,APP
R1796 T2722 T2719 conj tTA,APP
R1797 T2723 T2717 auxpass was,performed
R1798 T2724 T2717 prep in,performed
R1799 T2725 T2726 det the,reaction
R1800 T2726 T2724 pobj reaction,in
R1801 T2727 T2726 amod same,reaction
R1802 T2728 T2726 compound PCR,reaction
R1803 T2729 T2717 punct ", ",performed
R1804 T2730 T2717 advcl using,performed
R1805 T2731 T2732 nummod five,primers
R1806 T2732 T2730 dobj primers,using
R1807 T2733 T2732 amod separate,primers
R1808 T2734 T2717 punct .,performed
R1809 T2736 T2737 nsubjpass APP,amplified
R1810 T2738 T2737 auxpass was,amplified
R1811 T2739 T2737 advcl using,amplified
R1812 T2740 T2741 advmod forward,S36
R1813 T2741 T2739 dobj S36,using
R1814 T2742 T2741 compound primer,S36
R1815 T2743 T2741 acl located,S36
R1816 T2744 T2743 prep in,located
R1817 T2745 T2746 det the,end
R1818 T2746 T2744 pobj end,in
R1819 T2747 T2746 nummod 3,end
R1820 T2748 T2747 punct ′,3
R1821 T2749 T2746 prep of,end
R1822 T2750 T2751 det the,cDNA
R1823 T2751 T2749 pobj cDNA,of
R1824 T2752 T2751 compound APP,cDNA
R1825 T2753 T2754 punct (,ATG
R1826 T2754 T2751 parataxis ATG,cDNA
R1827 T2755 T2754 compound CCG,ATG
R1828 T2756 T2754 compound AGA,ATG
R1829 T2757 T2754 compound TCT,ATG
R1830 T2758 T2754 compound CTG,ATG
R1831 T2759 T2754 compound AAG,ATG
R1832 T2760 T2754 compound TGA,ATG
R1833 T2761 T2754 compound AGA,ATG
R1834 T2762 T2754 compound TGG,ATG
R1835 T2763 T2754 punct ),ATG
R1836 T2764 T2741 cc and,S36
R1837 T2765 T2766 amod reverse,J
R1838 T2766 T2741 conj J,S36
R1839 T2767 T2766 compound primer,J
R1840 T2768 T2766 compound PrP,J
R1841 T2769 T2766 punct -,J
R1842 T2770 T2766 compound AS,J
R1843 T2771 T2766 punct -,J
R1844 T2772 T2766 acl located,J
R1845 T2773 T2772 prep in,located
R1846 T2774 T2775 det the,UTR
R1847 T2775 T2773 pobj UTR,in
R1848 T2776 T2775 nummod 3,UTR
R1849 T2777 T2776 punct ′,3
R1850 T2778 T2775 prep of,UTR
R1851 T2779 T2780 det the,vector
R1852 T2780 T2778 pobj vector,of
R1853 T2781 T2782 punct (,GC
R1854 T2782 T2775 parataxis GC,UTR
R1855 T2783 T2782 compound CCA,GC
R1856 T2784 T2782 compound AGC,GC
R1857 T2785 T2782 compound CTA,GC
R1858 T2786 T2782 compound GAC,GC
R1859 T2787 T2782 compound CAC,GC
R1860 T2788 T2782 compound GAG,GC
R1861 T2789 T2782 compound AAT,GC
R1862 T2790 T2782 punct ),GC
R1863 T2791 T2737 punct .,amplified
R1864 T2793 T2794 det The,transgene
R1865 T2794 T2796 nsubjpass transgene,detected
R1866 T2795 T2794 compound tTA,transgene
R1867 T2797 T2796 auxpass was,detected
R1868 T2798 T2796 advcl using,detected
R1869 T2799 T2800 det a,set
R1870 T2800 T2798 dobj set,using
R1871 T2801 T2800 compound primer,set
R1872 T2802 T2803 dep that,amplified
R1873 T2803 T2800 relcl amplified,set
R1874 T2804 T2803 prep across,amplified
R1875 T2805 T2806 poss its,subdomains
R1876 T2806 T2804 pobj subdomains,across
R1877 T2807 T2806 nummod two,subdomains
R1878 T2808 T2803 prep with,amplified
R1879 T2809 T2808 pobj tet,with
R1880 T2810 T2809 advmod forward,tet
R1881 T2811 T2809 acl located,tet
R1882 T2812 T2811 prep within,located
R1883 T2813 T2814 det the,repressor
R1884 T2814 T2812 pobj repressor,within
R1885 T2815 T2816 compound Tn10,tetracycline
R1886 T2816 T2814 compound tetracycline,repressor
R1887 T2817 T2818 punct (,AG
R1888 T2818 T2814 parataxis AG,repressor
R1889 T2819 T2818 compound CGC,AG
R1890 T2820 T2818 compound TGT,AG
R1891 T2821 T2818 compound GGG,AG
R1892 T2822 T2818 compound GCA,AG
R1893 T2823 T2818 compound TTT,AG
R1894 T2824 T2818 compound TAC,AG
R1895 T2825 T2818 compound TTT,AG
R1896 T2826 T2818 punct ),AG
R1897 T2827 T2809 cc and,tet
R1898 T2828 T2809 conj tet,tet
R1899 T2829 T2828 amod reverse,tet
R1900 T2830 T2828 prep within,tet
R1901 T2831 T2832 det the,VP16
R1902 T2832 T2830 pobj VP16,within
R1903 T2833 T2832 compound HSV1,VP16
R1904 T2834 T2835 punct (,GTC
R1905 T2835 T2832 parataxis GTC,VP16
R1906 T2836 T2835 compound CAT,GTC
R1907 T2837 T2835 compound GTC,GTC
R1908 T2838 T2835 compound CAG,GTC
R1909 T2839 T2835 compound ATC,GTC
R1910 T2840 T2835 compound GAA,GTC
R1911 T2841 T2835 compound ATC,GTC
R1912 T2842 T2835 punct ),GTC
R1913 T2843 T2796 punct .,detected
R1914 T2845 T2846 det All,reactions
R1915 T2846 T2847 nsubj reactions,amplified
R1916 T2848 T2847 punct ", ",amplified
R1917 T2849 T2850 mark whether,positive
R1918 T2850 T2847 advcl positive,amplified
R1919 T2851 T2850 npadvmod transgene,positive
R1920 T2852 T2850 punct -,positive
R1921 T2853 T2850 cc or,positive
R1922 T2854 T2850 conj not,positive
R1923 T2855 T2847 punct ", ",amplified
R1924 T2856 T2857 det a,segment
R1925 T2857 T2847 dobj segment,amplified
R1926 T2858 T2857 prep of,segment
R1927 T2859 T2860 det the,gene
R1928 T2860 T2858 pobj gene,of
R1929 T2861 T2860 amod endogenous,gene
R1930 T2862 T2860 compound prion,gene
R1931 T2863 T2860 compound protein,gene
R1932 T2864 T2847 prep as,amplified
R1933 T2865 T2866 det a,control
R1934 T2866 T2864 pobj control,as
R1935 T2867 T2866 prep for,control
R1936 T2868 T2869 compound DNA,quality
R1937 T2869 T2867 pobj quality,for
R1938 T2870 T2847 advcl using,amplified
R1939 T2871 T2872 det a,primer
R1940 T2872 T2870 dobj primer,using
R1941 T2873 T2872 amod forward,primer
R1942 T2874 T2872 punct ", ",primer
R1943 T2875 T2876 compound PrP,J
R1944 T2876 T2872 appos J,primer
R1945 T2877 T2876 punct -,J
R1946 T2878 T2876 compound S,J
R1947 T2879 T2876 punct -,J
R1948 T2880 T2872 punct ", ",primer
R1949 T2881 T2872 amod specific,primer
R1950 T2882 T2881 prep to,specific
R1951 T2883 T2884 det the,frame
R1952 T2884 T2882 pobj frame,to
R1953 T2885 T2884 nmod mouse,frame
R1954 T2886 T2884 nmod PrP,frame
R1955 T2887 T2888 amod open,reading
R1956 T2888 T2884 compound reading,frame
R1957 T2889 T2890 punct (,GG
R1958 T2890 T2884 parataxis GG,frame
R1959 T2891 T2890 compound GGG,GG
R1960 T2892 T2890 compound ACT,GG
R1961 T2893 T2890 compound ATG,GG
R1962 T2894 T2890 compound TGG,GG
R1963 T2895 T2890 compound ACT,GG
R1964 T2896 T2890 compound GAT,GG
R1965 T2897 T2890 compound GTC,GG
R1966 T2898 T2890 punct ),GG
R1967 T2899 T2872 cc and,primer
R1968 T2900 T2901 det a,primer
R1969 T2901 T2872 conj primer,primer
R1970 T2902 T2901 amod reverse,primer
R1971 T2903 T2901 punct ", ",primer
R1972 T2904 T2905 compound PrP,J
R1973 T2905 T2901 appos J,primer
R1974 T2906 T2905 punct -,J
R1975 T2907 T2905 compound AS,J
R1976 T2908 T2905 punct -,J
R1977 T2909 T2901 punct ", ",primer
R1978 T2910 T2901 acl shared,primer
R1979 T2911 T2910 agent by,shared
R1980 T2912 T2913 det the,UTR
R1981 T2913 T2911 pobj UTR,by
R1982 T2914 T2913 nummod 3,UTR
R1983 T2915 T2914 punct ′,3
R1984 T2916 T2913 prep of,UTR
R1985 T2917 T2918 det the,gene
R1986 T2918 T2916 pobj gene,of
R1987 T2919 T2918 amod endogenous,gene
R1988 T2920 T2918 compound PrP,gene
R1989 T2921 T2918 cc and,gene
R1990 T2922 T2923 det the,vector
R1991 T2923 T2918 conj vector,gene
R1992 T2924 T2923 compound transgene,vector
R1993 T2925 T2847 punct .,amplified
R1994 T2927 T2928 compound Amplification,reactions
R1995 T2928 T2929 nsubjpass reactions,run
R1996 T2930 T2929 auxpass were,run
R1997 T2931 T2929 prep for,run
R1998 T2932 T2933 nummod 37,cycles
R1999 T2933 T2931 pobj cycles,for
R2000 T2934 T2929 prep at,run
R2001 T2935 T2936 nummod 94,°C
R2002 T2936 T2934 pobj °C,at
R2003 T2937 T2929 prep for,run
R2004 T2938 T2939 nummod 30,s
R2005 T2939 T2937 pobj s,for
R2006 T2940 T2929 punct ", ",run
R2007 T2941 T2942 nummod 64,°C
R2008 T2942 T2929 conj °C,run
R2009 T2943 T2942 prep for,°C
R2010 T2944 T2945 nummod 1,min
R2011 T2945 T2943 pobj min,for
R2012 T2946 T2942 punct ", ",°C
R2013 T2947 T2942 cc and,°C
R2014 T2948 T2949 nummod 72,°C
R2015 T2949 T2942 conj °C,°C
R2016 T2950 T2949 prep for,°C
R2017 T2951 T2952 nummod 1,min
R2018 T2952 T2950 pobj min,for
R2019 T2953 T2929 punct .,run
R2020 T2955 T2956 det All,samples
R2021 T2956 T2957 nsubj samples,gave
R2022 T2958 T2956 punct ", ",samples
R2023 T2959 T2956 amod transgenic,samples
R2024 T2960 T2959 cc and,transgenic
R2025 T2961 T2962 amod wild,type
R2026 T2962 T2959 conj type,transgenic
R2027 T2963 T2962 punct -,type
R2028 T2964 T2957 punct ", ",gave
R2029 T2965 T2966 det a,product
R2030 T2966 T2957 dobj product,gave
R2031 T2967 T2968 nummod 750,bp
R2032 T2968 T2966 compound bp,product
R2033 T2969 T2968 punct -,bp
R2034 T2970 T2966 prep from,product
R2035 T2971 T2972 det the,gene
R2036 T2972 T2970 pobj gene,from
R2037 T2973 T2972 amod endogenous,gene
R2038 T2974 T2972 compound PrP,gene
R2039 T2975 T2957 punct .,gave
R2040 T2977 T2978 det The,transgene
R2041 T2978 T2980 nsubj transgene,yielded
R2042 T2979 T2978 compound APP,transgene
R2043 T2980 T2981 ccomp yielded,fell
R2044 T2982 T2983 det an,band
R2045 T2983 T2980 dobj band,yielded
R2046 T2984 T2983 amod additional,band
R2047 T2985 T2983 prep at,band
R2048 T2986 T2987 nummod 400,bp
R2049 T2987 T2985 pobj bp,at
R2050 T2988 T2981 punct ;,fell
R2051 T2989 T2990 det the,product
R2052 T2990 T2981 nsubj product,fell
R2053 T2991 T2990 compound tTA,product
R2054 T2992 T2993 advmod in,between
R2055 T2993 T2981 advmod between,fell
R2056 T2994 T2981 prep at,fell
R2057 T2995 T2996 nummod 480,bp
R2058 T2996 T2994 pobj bp,at
R2059 T2997 T2981 punct .,fell