craft-sa-dev Home  

PMC:1283364 / 14270-17613 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T4137 0-7 NN denotes Amyloid
T4138 8-17 NN denotes Histology
T4139 17-131 sentence denotes Mice were euthanized by ether inhalation and brains removed for immersion fixation in 4% paraformaldehyde/1× PBS.
T4140 18-22 NNS denotes Mice
T4142 23-27 VBD denotes were
T4141 28-38 VBN denotes euthanized
T4143 39-41 IN denotes by
T4144 42-47 NN denotes ether
T4145 48-58 NN denotes inhalation
T4146 59-62 CC denotes and
T4147 63-69 NNS denotes brains
T4148 70-77 VBN denotes removed
T4149 78-81 IN denotes for
T4150 82-91 NN denotes immersion
T4151 92-100 NN denotes fixation
T4152 101-103 IN denotes in
T4153 104-105 CD denotes 4
T4154 105-106 NN denotes %
T4155 107-123 NN denotes paraformaldehyde
T4156 123-124 HYPH denotes /
T4157 124-125 CD denotes 1
T4159 125-126 SYM denotes ×
T4158 127-130 NN denotes PBS
T4160 130-131 . denotes .
T4161 131-309 sentence denotes After 48 h in fixative at 4 °C, brains were transferred to PBS, dehydrated in alcohols, treated with cedarwood oil and methylsalicylate, and embedded in paraffin for sectioning.
T4162 132-137 IN denotes After
T4164 138-140 CD denotes 48
T4165 141-142 NN denotes h
T4166 143-145 IN denotes in
T4167 146-154 JJ denotes fixative
T4168 155-157 IN denotes at
T4169 158-159 CD denotes 4
T4170 160-162 NN denotes °C
T4171 162-164 , denotes ,
T4172 164-170 NNS denotes brains
T4173 171-175 VBD denotes were
T4163 176-187 VBN denotes transferred
T4174 188-190 IN denotes to
T4175 191-194 NN denotes PBS
T4176 194-196 , denotes ,
T4177 196-206 VBN denotes dehydrated
T4178 207-209 IN denotes in
T4179 210-218 NNS denotes alcohols
T4180 218-220 , denotes ,
T4181 220-227 VBN denotes treated
T4182 228-232 IN denotes with
T4183 233-242 NN denotes cedarwood
T4184 243-246 NN denotes oil
T4185 247-250 CC denotes and
T4186 251-267 NN denotes methylsalicylate
T4187 267-269 , denotes ,
T4188 269-272 CC denotes and
T4189 273-281 VBN denotes embedded
T4190 282-284 IN denotes in
T4191 285-293 NN denotes paraffin
T4192 294-297 IN denotes for
T4193 298-308 VBG denotes sectioning
T4194 308-309 . denotes .
T4195 309-330 sentence denotes Hirano silver stain
T4196 311-317 NN denotes Hirano
T4198 318-324 NN denotes silver
T4197 325-330 NN denotes stain
T4199 330-468 sentence denotes Silver impregnation histology was performed on 10-μm paraffin-embedded sections by Hirano's modification of the Bielschowsky method [21].
T4200 331-337 NN denotes Silver
T4202 338-350 NN denotes impregnation
T4201 351-360 NN denotes histology
T4204 361-364 VBD denotes was
T4203 365-374 VBN denotes performed
T4205 375-377 IN denotes on
T4206 378-380 CD denotes 10
T4208 380-381 HYPH denotes -
T4207 381-383 NN denotes μm
T4210 384-392 NN denotes paraffin
T4212 392-393 HYPH denotes -
T4211 393-401 VBN denotes embedded
T4209 402-410 NNS denotes sections
T4213 411-413 IN denotes by
T4214 414-420 NNP denotes Hirano
T4216 420-422 POS denotes 's
T4215 423-435 NN denotes modification
T4217 436-438 IN denotes of
T4218 439-442 DT denotes the
T4220 443-455 NNP denotes Bielschowsky
T4219 456-462 NN denotes method
T4221 463-464 -LRB- denotes [
T4222 464-466 CD denotes 21
T4223 466-467 -RRB- denotes ]
T4224 467-468 . denotes .
T4225 468-620 sentence denotes Briefly, sections were deparaffinized through xylene and alcohols into tap water before being placed into fresh 20% silver nitrate solution for 20 min.
T4226 469-476 RB denotes Briefly
T4228 476-478 , denotes ,
T4229 478-486 NNS denotes sections
T4230 487-491 VBD denotes were
T4227 492-506 VBN denotes deparaffinized
T4231 507-514 IN denotes through
T4232 515-521 NN denotes xylene
T4233 522-525 CC denotes and
T4234 526-534 NNS denotes alcohols
T4235 535-539 IN denotes into
T4236 540-543 NN denotes tap
T4237 544-549 NN denotes water
T4238 550-556 IN denotes before
T4239 557-562 VBG denotes being
T4240 563-569 VBN denotes placed
T4241 570-574 IN denotes into
T4242 575-580 JJ denotes fresh
T4244 581-583 CD denotes 20
T4245 583-584 NN denotes %
T4246 585-591 NN denotes silver
T4247 592-599 NN denotes nitrate
T4243 600-608 NN denotes solution
T4248 609-612 IN denotes for
T4249 613-615 CD denotes 20
T4250 616-619 NN denotes min
T4251 619-620 . denotes .
T4252 620-764 sentence denotes After being washed thoroughly with distilled water, slides were immersed in 20% silver nitrate solution titrated with fresh ammonium hydroxide.
T4253 621-626 IN denotes After
T4255 627-632 VBG denotes being
T4256 633-639 VBN denotes washed
T4257 640-650 RB denotes thoroughly
T4258 651-655 IN denotes with
T4259 656-665 JJ denotes distilled
T4260 666-671 NN denotes water
T4261 671-673 , denotes ,
T4262 673-679 NNS denotes slides
T4263 680-684 VBD denotes were
T4254 685-693 VBN denotes immersed
T4264 694-696 IN denotes in
T4265 697-699 CD denotes 20
T4266 699-700 NN denotes %
T4268 701-707 NN denotes silver
T4269 708-715 NN denotes nitrate
T4267 716-724 NN denotes solution
T4270 725-733 VBN denotes titrated
T4271 734-738 IN denotes with
T4272 739-744 JJ denotes fresh
T4274 745-753 NN denotes ammonium
T4273 754-763 NN denotes hydroxide
T4275 763-764 . denotes .
T4276 764-1045 sentence denotes After 20 min, slides were washed with ammonia water before being individually developed with 100 μl of developer (20 ml of 37% formaldehyde, 100 ml of distilled water, 50 μl of concentrated nitric acid, and 0.5 g of citric acid) added to 50 ml of titrated silver nitrate solution.
T4277 765-770 IN denotes After
T4279 771-773 CD denotes 20
T4280 774-777 NN denotes min
T4281 777-779 , denotes ,
T4282 779-785 NNS denotes slides
T4283 786-790 VBD denotes were
T4278 791-797 VBN denotes washed
T4284 798-802 IN denotes with
T4285 803-810 NN denotes ammonia
T4286 811-816 NN denotes water
T4287 817-823 IN denotes before
T4288 824-829 VBG denotes being
T4290 830-842 RB denotes individually
T4289 843-852 VBN denotes developed
T4291 853-857 IN denotes with
T4292 858-861 CD denotes 100
T4293 862-864 NN denotes μl
T4294 865-867 IN denotes of
T4295 868-877 NN denotes developer
T4296 878-879 -LRB- denotes (
T4298 879-881 CD denotes 20
T4297 882-884 NN denotes ml
T4299 885-887 IN denotes of
T4300 888-890 CD denotes 37
T4301 890-891 NN denotes %
T4302 892-904 NN denotes formaldehyde
T4303 904-906 , denotes ,
T4304 906-909 CD denotes 100
T4305 910-912 NN denotes ml
T4306 913-915 IN denotes of
T4307 916-925 JJ denotes distilled
T4308 926-931 NN denotes water
T4309 931-933 , denotes ,
T4310 933-935 CD denotes 50
T4311 936-938 NN denotes μl
T4312 939-941 IN denotes of
T4313 942-954 VBN denotes concentrated
T4315 955-961 JJ denotes nitric
T4314 962-966 NN denotes acid
T4316 966-968 , denotes ,
T4317 968-971 CC denotes and
T4318 972-975 CD denotes 0.5
T4319 976-977 NN denotes g
T4320 978-980 IN denotes of
T4321 981-987 JJ denotes citric
T4322 988-992 NN denotes acid
T4323 992-993 -RRB- denotes )
T4324 994-999 VBN denotes added
T4325 1000-1002 IN denotes to
T4326 1003-1005 CD denotes 50
T4327 1006-1008 NN denotes ml
T4328 1009-1011 IN denotes of
T4329 1012-1020 VBN denotes titrated
T4331 1021-1027 NN denotes silver
T4332 1028-1035 NN denotes nitrate
T4330 1036-1044 NN denotes solution
T4333 1044-1045 . denotes .
T4334 1045-1159 sentence denotes Slides were then rinsed in tap water, fixed in 5% sodium thiosulfate, and dehydrated through alcohols and xylene.
T4335 1046-1052 NNS denotes Slides
T4337 1053-1057 VBD denotes were
T4338 1058-1062 RB denotes then
T4336 1063-1069 VBN denotes rinsed
T4339 1070-1072 IN denotes in
T4340 1073-1076 NN denotes tap
T4341 1077-1082 NN denotes water
T4342 1082-1084 , denotes ,
T4343 1084-1089 VBN denotes fixed
T4344 1090-1092 IN denotes in
T4345 1093-1094 CD denotes 5
T4346 1094-1095 NN denotes %
T4348 1096-1102 NN denotes sodium
T4347 1103-1114 NN denotes thiosulfate
T4349 1114-1116 , denotes ,
T4350 1116-1119 CC denotes and
T4351 1120-1130 VBN denotes dehydrated
T4352 1131-1138 IN denotes through
T4353 1139-1147 NNS denotes alcohols
T4354 1148-1151 CC denotes and
T4355 1152-1158 NN denotes xylene
T4356 1158-1159 . denotes .
T4357 1159-1182 sentence denotes Thioflavin-S staining
T4358 1161-1171 NN denotes Thioflavin
T4360 1171-1172 HYPH denotes -
T4359 1172-1173 NN denotes S
T4361 1174-1182 NN denotes staining
T4362 1182-1368 sentence denotes Following deparaffinization of sections through xylene and alcohols, amyloid impregnation with thioflavin-S was performed according to the Guntern modification of the standard protocol.
T4363 1183-1192 VBG denotes Following
T4365 1193-1210 NN denotes deparaffinization
T4366 1211-1213 IN denotes of
T4367 1214-1222 NNS denotes sections
T4368 1223-1230 IN denotes through
T4369 1231-1237 NN denotes xylene
T4370 1238-1241 CC denotes and
T4371 1242-1250 NNS denotes alcohols
T4372 1250-1252 , denotes ,
T4373 1252-1259 NN denotes amyloid
T4374 1260-1272 NN denotes impregnation
T4375 1273-1277 IN denotes with
T4376 1278-1288 NN denotes thioflavin
T4378 1288-1289 HYPH denotes -
T4377 1289-1290 NN denotes S
T4379 1291-1294 VBD denotes was
T4364 1295-1304 VBN denotes performed
T4380 1305-1314 VBG denotes according
T4381 1315-1317 IN denotes to
T4382 1318-1321 DT denotes the
T4384 1322-1329 NN denotes Guntern
T4383 1330-1342 NN denotes modification
T4385 1343-1345 IN denotes of
T4386 1346-1349 DT denotes the
T4388 1350-1358 JJ denotes standard
T4387 1359-1367 NN denotes protocol
T4389 1367-1368 . denotes .
T4390 1368-1588 sentence denotes Slides holding 10-μm paraffin sections were washed twice in distilled water, then immersed for 5 min in a 0.25% potassium permanganate solution, followed by 5 min in a 1% potassium metabisulfate/1% oxalic acid solution.
T4391 1369-1375 NNS denotes Slides
T4393 1376-1383 VBG denotes holding
T4394 1384-1386 CD denotes 10
T4396 1386-1387 HYPH denotes -
T4395 1387-1389 NN denotes μm
T4398 1390-1398 NN denotes paraffin
T4397 1399-1407 NNS denotes sections
T4399 1408-1412 VBD denotes were
T4392 1413-1419 VBN denotes washed
T4400 1420-1425 RB denotes twice
T4401 1426-1428 IN denotes in
T4402 1429-1438 JJ denotes distilled
T4403 1439-1444 NN denotes water
T4404 1444-1446 , denotes ,
T4405 1446-1450 RB denotes then
T4406 1451-1459 VBN denotes immersed
T4407 1460-1463 IN denotes for
T4408 1464-1465 CD denotes 5
T4409 1466-1469 NN denotes min
T4410 1470-1472 IN denotes in
T4411 1473-1474 DT denotes a
T4413 1475-1479 CD denotes 0.25
T4414 1479-1480 NN denotes %
T4415 1481-1490 NN denotes potassium
T4416 1491-1503 NN denotes permanganate
T4412 1504-1512 NN denotes solution
T4417 1512-1514 , denotes ,
T4418 1514-1522 VBN denotes followed
T4419 1523-1525 IN denotes by
T4420 1526-1527 CD denotes 5
T4421 1528-1531 NN denotes min
T4422 1532-1534 IN denotes in
T4423 1535-1536 DT denotes a
T4425 1537-1538 CD denotes 1
T4426 1538-1539 NN denotes %
T4428 1540-1549 NN denotes potassium
T4427 1550-1563 NN denotes metabisulfate
T4429 1563-1564 HYPH denotes /
T4430 1564-1565 CD denotes 1
T4431 1565-1566 NN denotes %
T4433 1567-1573 JJ denotes oxalic
T4432 1574-1578 NN denotes acid
T4424 1579-1587 NN denotes solution
T4434 1587-1588 . denotes .
T4435 1588-1740 sentence denotes After this preparation, slides were placed into a filtered aqueous 0.02% thioflavin-S solution (Chroma-Gesellschaft Schmid, Kongen, Germany) for 8 min.
T4436 1589-1594 IN denotes After
T4438 1595-1599 DT denotes this
T4439 1600-1611 NN denotes preparation
T4440 1611-1613 , denotes ,
T4441 1613-1619 NNS denotes slides
T4442 1620-1624 VBD denotes were
T4437 1625-1631 VBN denotes placed
T4443 1632-1636 IN denotes into
T4444 1637-1638 DT denotes a
T4446 1639-1647 VBN denotes filtered
T4447 1648-1655 JJ denotes aqueous
T4448 1656-1660 CD denotes 0.02
T4449 1660-1661 NN denotes %
T4450 1662-1672 NN denotes thioflavin
T4452 1672-1673 HYPH denotes -
T4451 1673-1674 NN denotes S
T4445 1675-1683 NN denotes solution
T4453 1684-1685 -LRB- denotes (
T4455 1685-1691 NNP denotes Chroma
T4457 1691-1692 HYPH denotes -
T4456 1692-1704 NNP denotes Gesellschaft
T4454 1705-1711 NNP denotes Schmid
T4458 1711-1713 , denotes ,
T4459 1713-1719 NNP denotes Kongen
T4460 1719-1721 , denotes ,
T4461 1721-1728 NNP denotes Germany
T4462 1728-1729 -RRB- denotes )
T4463 1730-1733 IN denotes for
T4464 1734-1735 CD denotes 8
T4465 1736-1739 NN denotes min
T4466 1739-1740 . denotes .
T4467 1740-1924 sentence denotes Excess stain was removed by two brief rinses in 80% ethanol, then two in distilled water, after which slides were finished in aqueous mounting medium for florescence photomicrography.
T4468 1741-1747 JJ denotes Excess
T4469 1748-1753 NN denotes stain
T4471 1754-1757 VBD denotes was
T4470 1758-1765 VBN denotes removed
T4472 1766-1768 IN denotes by
T4473 1769-1772 CD denotes two
T4475 1773-1778 JJ denotes brief
T4474 1779-1785 NNS denotes rinses
T4476 1786-1788 IN denotes in
T4477 1789-1791 CD denotes 80
T4478 1791-1792 NN denotes %
T4479 1793-1800 NN denotes ethanol
T4480 1800-1802 , denotes ,
T4481 1802-1806 RB denotes then
T4482 1807-1810 CD denotes two
T4483 1811-1813 IN denotes in
T4484 1814-1823 JJ denotes distilled
T4485 1824-1829 NN denotes water
T4486 1829-1831 , denotes ,
T4487 1831-1836 IN denotes after
T4489 1837-1842 WDT denotes which
T4490 1843-1849 NNS denotes slides
T4491 1850-1854 VBD denotes were
T4488 1855-1863 VBN denotes finished
T4492 1864-1866 IN denotes in
T4493 1867-1874 JJ denotes aqueous
T4495 1875-1883 NN denotes mounting
T4494 1884-1890 NN denotes medium
T4496 1891-1894 IN denotes for
T4497 1895-1906 NN denotes florescence
T4498 1907-1923 NN denotes photomicrography
T4499 1923-1924 . denotes .
T4500 1924-1997 sentence denotes Ubiquitin, glial fibrillary acidic protein, and Aβ immunohistochemistry
T4501 1926-1935 NN denotes Ubiquitin
T4502 1935-1937 , denotes ,
T4503 1937-1942 JJ denotes glial
T4505 1943-1953 JJ denotes fibrillary
T4506 1954-1960 JJ denotes acidic
T4504 1961-1968 NN denotes protein
T4507 1968-1970 , denotes ,
T4508 1970-1973 CC denotes and
T4509 1974-1976 NN denotes Aβ
T4510 1977-1997 NN denotes immunohistochemistry
T4511 1997-2098 sentence denotes Prior to immunostaining, slides were deparaffinized by oven heating followed by immersion in xylene.
T4512 1998-2003 JJ denotes Prior
T4513 2004-2006 IN denotes to
T4515 2007-2021 NN denotes immunostaining
T4516 2021-2023 , denotes ,
T4517 2023-2029 NNS denotes slides
T4518 2030-2034 VBD denotes were
T4514 2035-2049 VBN denotes deparaffinized
T4519 2050-2052 IN denotes by
T4520 2053-2057 NN denotes oven
T4521 2058-2065 NN denotes heating
T4522 2066-2074 VBN denotes followed
T4523 2075-2077 IN denotes by
T4524 2078-2087 NN denotes immersion
T4525 2088-2090 IN denotes in
T4526 2091-2097 NN denotes xylene
T4527 2097-2098 . denotes .
T4528 2098-2253 sentence denotes After rehydration through graded alcohols into tap water, endogenous peroxidase activity was quenched by incubation with 3% hydrogen peroxide in methanol.
T4529 2099-2104 IN denotes After
T4531 2105-2116 NN denotes rehydration
T4532 2117-2124 IN denotes through
T4533 2125-2131 VBN denotes graded
T4534 2132-2140 NNS denotes alcohols
T4535 2141-2145 IN denotes into
T4536 2146-2149 NN denotes tap
T4537 2150-2155 NN denotes water
T4538 2155-2157 , denotes ,
T4539 2157-2167 JJ denotes endogenous
T4541 2168-2178 NN denotes peroxidase
T4540 2179-2187 NN denotes activity
T4542 2188-2191 VBD denotes was
T4530 2192-2200 VBN denotes quenched
T4543 2201-2203 IN denotes by
T4544 2204-2214 NN denotes incubation
T4545 2215-2219 IN denotes with
T4546 2220-2221 CD denotes 3
T4547 2221-2222 NN denotes %
T4549 2223-2231 NN denotes hydrogen
T4548 2232-2240 NN denotes peroxide
T4550 2241-2243 IN denotes in
T4551 2244-2252 NN denotes methanol
T4552 2252-2253 . denotes .
T4553 2253-2336 sentence denotes Slides were microwaved for 5–7 min in water, cooled for 5 min, then washed in TBS.
T4554 2254-2260 NNS denotes Slides
T4556 2261-2265 VBD denotes were
T4555 2266-2276 VBN denotes microwaved
T4557 2277-2280 IN denotes for
T4558 2281-2282 CD denotes 5
T4560 2282-2283 SYM denotes –
T4559 2283-2284 CD denotes 7
T4561 2285-2288 NN denotes min
T4562 2289-2291 IN denotes in
T4563 2292-2297 NN denotes water
T4564 2297-2299 , denotes ,
T4565 2299-2305 VBN denotes cooled
T4566 2306-2309 IN denotes for
T4567 2310-2311 CD denotes 5
T4568 2312-2315 NN denotes min
T4569 2315-2317 , denotes ,
T4570 2317-2321 RB denotes then
T4571 2322-2328 VBN denotes washed
T4572 2329-2331 IN denotes in
T4573 2332-2335 NN denotes TBS
T4574 2335-2336 . denotes .
T4575 2336-2433 sentence denotes Nonspecific staining was blocked for 1 h with 3% normal goat serum and 0.1% Triton-X 100 in TBS.
T4576 2337-2348 JJ denotes Nonspecific
T4577 2349-2357 NN denotes staining
T4579 2358-2361 VBD denotes was
T4578 2362-2369 VBN denotes blocked
T4580 2370-2373 IN denotes for
T4581 2374-2375 CD denotes 1
T4582 2376-2377 NN denotes h
T4583 2378-2382 IN denotes with
T4584 2383-2384 CD denotes 3
T4585 2384-2385 NN denotes %
T4587 2386-2392 JJ denotes normal
T4588 2393-2397 NN denotes goat
T4586 2398-2403 NN denotes serum
T4589 2404-2407 CC denotes and
T4590 2408-2411 CD denotes 0.1
T4591 2411-2412 NN denotes %
T4593 2413-2419 NN denotes Triton
T4594 2419-2420 HYPH denotes -
T4592 2420-2421 NN denotes X
T4595 2422-2425 CD denotes 100
T4596 2426-2428 IN denotes in
T4597 2429-2432 NN denotes TBS
T4598 2432-2433 . denotes .
T4599 2433-2830 sentence denotes Slides were then placed into primary antibody (rabbit anti-Aβ peptide polyclonal antibody, Zymed Laboratories, South San Francisco, California, United States; rabbit anti-ubiquitin and rabbit anti–glial fibrillary acidic protein (GFAP) polyclonal antibodies, Dako, Carpinteria, California, United States) diluted 1:500 in TBS with 2% normal goat serum and incubated overnight at room temperature.
T4600 2434-2440 NNS denotes Slides
T4602 2441-2445 VBD denotes were
T4603 2446-2450 RB denotes then
T4601 2451-2457 VBN denotes placed
T4604 2458-2462 IN denotes into
T4605 2463-2470 JJ denotes primary
T4606 2471-2479 NN denotes antibody
T4607 2480-2481 -LRB- denotes (
T4609 2481-2487 NN denotes rabbit
T4611 2488-2495 JJ denotes anti-Aβ
T4612 2496-2503 NN denotes peptide
T4613 2504-2514 JJ denotes polyclonal
T4610 2515-2523 NN denotes antibody
T4614 2523-2525 , denotes ,
T4615 2525-2530 NNP denotes Zymed
T4616 2531-2543 NNP denotes Laboratories
T4617 2543-2545 , denotes ,
T4618 2545-2550 NNP denotes South
T4620 2551-2554 NNP denotes San
T4619 2555-2564 NNP denotes Francisco
T4621 2564-2566 , denotes ,
T4622 2566-2576 NNP denotes California
T4623 2576-2578 , denotes ,
T4624 2578-2584 NNP denotes United
T4625 2585-2591 NNP denotes States
T4626 2591-2592 : denotes ;
T4627 2593-2599 NN denotes rabbit
T4628 2600-2614 JJ denotes anti-ubiquitin
T4629 2615-2618 CC denotes and
T4630 2619-2625 NN denotes rabbit
T4631 2626-2636 JJ denotes anti–glial
T4632 2637-2647 JJ denotes fibrillary
T4634 2648-2654 JJ denotes acidic
T4633 2655-2662 NN denotes protein
T4635 2663-2664 -LRB- denotes (
T4636 2664-2668 NN denotes GFAP
T4637 2668-2669 -RRB- denotes )
T4638 2670-2680 JJ denotes polyclonal
T4608 2681-2691 NNS denotes antibodies
T4639 2691-2693 , denotes ,
T4640 2693-2697 NNP denotes Dako
T4641 2697-2699 , denotes ,
T4642 2699-2710 NNP denotes Carpinteria
T4643 2710-2712 , denotes ,
T4644 2712-2722 NNP denotes California
T4645 2722-2724 , denotes ,
T4646 2724-2730 NNP denotes United
T4647 2731-2737 NNP denotes States
T4648 2737-2738 -RRB- denotes )
T4649 2739-2746 VBN denotes diluted
T4650 2747-2748 CD denotes 1
T4651 2748-2749 SYM denotes :
T4652 2749-2752 CD denotes 500
T4653 2753-2755 IN denotes in
T4654 2756-2759 NN denotes TBS
T4655 2760-2764 IN denotes with
T4656 2765-2766 CD denotes 2
T4657 2766-2767 NN denotes %
T4659 2768-2774 JJ denotes normal
T4660 2775-2779 NN denotes goat
T4658 2780-2785 NN denotes serum
T4661 2786-2789 CC denotes and
T4662 2790-2799 VBN denotes incubated
T4663 2800-2809 RB denotes overnight
T4664 2810-2812 IN denotes at
T4665 2813-2817 NN denotes room
T4666 2818-2829 NN denotes temperature
T4667 2829-2830 . denotes .
T4668 2830-3237 sentence denotes After being washed of excess primary antibody with several changes of TBS, slides were incubated with either the Vectastain Elite anti-rabbit secondary system (anti-Aβ; Vector Laboratories, Burlingame, California, United States) or peroxidase/anti-peroxidase reagents (anti-ubitquitin and anti-GFAP; Sternberger Monoclonals, Lutherville, Maryland, United States) according to the manufacturers' directions.
T4669 2831-2836 IN denotes After
T4671 2837-2842 VBG denotes being
T4672 2843-2849 VBN denotes washed
T4673 2850-2852 IN denotes of
T4674 2853-2859 JJ denotes excess
T4676 2860-2867 JJ denotes primary
T4675 2868-2876 NN denotes antibody
T4677 2877-2881 IN denotes with
T4678 2882-2889 JJ denotes several
T4679 2890-2897 NNS denotes changes
T4680 2898-2900 IN denotes of
T4681 2901-2904 NN denotes TBS
T4682 2904-2906 , denotes ,
T4683 2906-2912 NNS denotes slides
T4684 2913-2917 VBD denotes were
T4670 2918-2927 VBN denotes incubated
T4685 2928-2932 IN denotes with
T4686 2933-2939 CC denotes either
T4688 2940-2943 DT denotes the
T4689 2944-2954 NNP denotes Vectastain
T4690 2955-2960 NNP denotes Elite
T4691 2961-2972 JJ denotes anti-rabbit
T4692 2973-2982 JJ denotes secondary
T4687 2983-2989 NN denotes system
T4693 2990-2991 -LRB- denotes (
T4695 2991-2998 JJ denotes anti-Aβ
T4696 2998-2999 : denotes ;
T4697 3000-3006 NNP denotes Vector
T4694 3007-3019 NNP denotes Laboratories
T4698 3019-3021 , denotes ,
T4699 3021-3031 NNP denotes Burlingame
T4700 3031-3033 , denotes ,
T4701 3033-3043 NNP denotes California
T4702 3043-3045 , denotes ,
T4703 3045-3051 NNP denotes United
T4704 3052-3058 NNP denotes States
T4705 3058-3059 -RRB- denotes )
T4706 3060-3062 CC denotes or
T4707 3063-3073 NN denotes peroxidase
T4709 3073-3074 HYPH denotes /
T4710 3074-3089 JJ denotes anti-peroxidase
T4708 3090-3098 NNS denotes reagents
T4711 3099-3100 -LRB- denotes (
T4713 3100-3115 JJ denotes anti-ubitquitin
T4714 3116-3119 CC denotes and
T4715 3120-3129 JJ denotes anti-GFAP
T4716 3129-3130 : denotes ;
T4717 3131-3142 NNP denotes Sternberger
T4712 3143-3154 NNPS denotes Monoclonals
T4718 3154-3156 , denotes ,
T4719 3156-3167 NNP denotes Lutherville
T4720 3167-3169 , denotes ,
T4721 3169-3177 NNP denotes Maryland
T4722 3177-3179 , denotes ,
T4723 3179-3185 NNP denotes United
T4724 3186-3192 NNP denotes States
T4725 3192-3193 -RRB- denotes )
T4726 3194-3203 VBG denotes according
T4727 3204-3206 IN denotes to
T4728 3207-3210 DT denotes the
T4729 3211-3224 NNS denotes manufacturers
T4731 3224-3225 POS denotes '
T4730 3226-3236 NNS denotes directions
T4732 3236-3237 . denotes .
T4733 3237-3343 sentence denotes Antibody binding was visualized with diaminobenzidene, and sections were counterstained with hematoxylin.
T4734 3238-3246 NN denotes Antibody
T4735 3247-3254 NN denotes binding
T4737 3255-3258 VBD denotes was
T4736 3259-3269 VBN denotes visualized
T4738 3270-3274 IN denotes with
T4739 3275-3291 NN denotes diaminobenzidene
T4740 3291-3293 , denotes ,
T4741 3293-3296 CC denotes and
T4742 3297-3305 NNS denotes sections
T4744 3306-3310 VBD denotes were
T4743 3311-3325 VBN denotes counterstained
T4745 3326-3330 IN denotes with
T4746 3331-3342 NN denotes hematoxylin
T4747 3342-3343 . denotes .
R2715 T4137 T4138 compound Amyloid,Histology
R2716 T4140 T4141 nsubjpass Mice,euthanized
R2717 T4142 T4141 auxpass were,euthanized
R2718 T4143 T4141 prep by,euthanized
R2719 T4144 T4145 compound ether,inhalation
R2720 T4145 T4143 pobj inhalation,by
R2721 T4146 T4141 cc and,euthanized
R2722 T4147 T4148 nsubj brains,removed
R2723 T4148 T4141 conj removed,euthanized
R2724 T4149 T4148 prep for,removed
R2725 T4150 T4151 compound immersion,fixation
R2726 T4151 T4149 pobj fixation,for
R2727 T4152 T4151 prep in,fixation
R2728 T4153 T4154 nummod 4,%
R2729 T4154 T4155 compound %,paraformaldehyde
R2730 T4155 T4152 pobj paraformaldehyde,in
R2731 T4156 T4155 punct /,paraformaldehyde
R2732 T4157 T4158 nummod 1,PBS
R2733 T4158 T4155 appos PBS,paraformaldehyde
R2734 T4159 T4157 punct ×,1
R2735 T4160 T4148 punct .,removed
R2736 T4162 T4163 prep After,transferred
R2737 T4164 T4165 nummod 48,h
R2738 T4165 T4162 pobj h,After
R2739 T4166 T4165 prep in,h
R2740 T4167 T4166 pobj fixative,in
R2741 T4168 T4165 prep at,h
R2742 T4169 T4170 nummod 4,°C
R2743 T4170 T4168 pobj °C,at
R2744 T4171 T4163 punct ", ",transferred
R2745 T4172 T4163 nsubjpass brains,transferred
R2746 T4173 T4163 auxpass were,transferred
R2747 T4174 T4163 prep to,transferred
R2748 T4175 T4174 pobj PBS,to
R2749 T4176 T4163 punct ", ",transferred
R2750 T4177 T4163 conj dehydrated,transferred
R2751 T4178 T4177 prep in,dehydrated
R2752 T4179 T4178 pobj alcohols,in
R2753 T4180 T4177 punct ", ",dehydrated
R2754 T4181 T4177 conj treated,dehydrated
R2755 T4182 T4181 prep with,treated
R2756 T4183 T4184 compound cedarwood,oil
R2757 T4184 T4182 pobj oil,with
R2758 T4185 T4184 cc and,oil
R2759 T4186 T4184 conj methylsalicylate,oil
R2760 T4187 T4181 punct ", ",treated
R2761 T4188 T4181 cc and,treated
R2762 T4189 T4181 conj embedded,treated
R2763 T4190 T4189 prep in,embedded
R2764 T4191 T4190 pobj paraffin,in
R2765 T4192 T4189 prep for,embedded
R2766 T4193 T4192 pobj sectioning,for
R2767 T4194 T4163 punct .,transferred
R2768 T4196 T4197 compound Hirano,stain
R2769 T4198 T4197 compound silver,stain
R2770 T4200 T4201 compound Silver,histology
R2771 T4201 T4203 nsubjpass histology,performed
R2772 T4202 T4201 compound impregnation,histology
R2773 T4204 T4203 auxpass was,performed
R2774 T4205 T4203 prep on,performed
R2775 T4206 T4207 nummod 10,μm
R2776 T4207 T4209 nmod μm,sections
R2777 T4208 T4207 punct -,μm
R2778 T4209 T4205 pobj sections,on
R2779 T4210 T4211 npadvmod paraffin,embedded
R2780 T4211 T4209 amod embedded,sections
R2781 T4212 T4211 punct -,embedded
R2782 T4213 T4203 prep by,performed
R2783 T4214 T4215 poss Hirano,modification
R2784 T4215 T4213 pobj modification,by
R2785 T4216 T4214 case 's,Hirano
R2786 T4217 T4215 prep of,modification
R2787 T4218 T4219 det the,method
R2788 T4219 T4217 pobj method,of
R2789 T4220 T4219 compound Bielschowsky,method
R2790 T4221 T4222 punct [,21
R2791 T4222 T4203 parataxis 21,performed
R2792 T4223 T4222 punct ],21
R2793 T4224 T4203 punct .,performed
R2794 T4226 T4227 advmod Briefly,deparaffinized
R2795 T4228 T4227 punct ", ",deparaffinized
R2796 T4229 T4227 nsubjpass sections,deparaffinized
R2797 T4230 T4227 auxpass were,deparaffinized
R2798 T4231 T4227 prep through,deparaffinized
R2799 T4232 T4231 pobj xylene,through
R2800 T4233 T4232 cc and,xylene
R2801 T4234 T4232 conj alcohols,xylene
R2802 T4235 T4227 prep into,deparaffinized
R2803 T4236 T4237 compound tap,water
R2804 T4237 T4235 pobj water,into
R2805 T4238 T4227 prep before,deparaffinized
R2806 T4239 T4240 auxpass being,placed
R2807 T4240 T4238 pcomp placed,before
R2808 T4241 T4240 prep into,placed
R2809 T4242 T4243 amod fresh,solution
R2810 T4243 T4241 pobj solution,into
R2811 T4244 T4245 nummod 20,%
R2812 T4245 T4243 compound %,solution
R2813 T4246 T4243 compound silver,solution
R2814 T4247 T4243 compound nitrate,solution
R2815 T4248 T4240 prep for,placed
R2816 T4249 T4250 nummod 20,min
R2817 T4250 T4248 pobj min,for
R2818 T4251 T4227 punct .,deparaffinized
R2819 T4253 T4254 prep After,immersed
R2820 T4255 T4256 auxpass being,washed
R2821 T4256 T4253 pcomp washed,After
R2822 T4257 T4256 advmod thoroughly,washed
R2823 T4258 T4256 prep with,washed
R2824 T4259 T4260 amod distilled,water
R2825 T4260 T4258 pobj water,with
R2826 T4261 T4254 punct ", ",immersed
R2827 T4262 T4254 nsubjpass slides,immersed
R2828 T4263 T4254 auxpass were,immersed
R2829 T4264 T4254 prep in,immersed
R2830 T4265 T4266 nummod 20,%
R2831 T4266 T4267 compound %,solution
R2832 T4267 T4264 pobj solution,in
R2833 T4268 T4269 compound silver,nitrate
R2834 T4269 T4267 compound nitrate,solution
R2835 T4270 T4267 acl titrated,solution
R2836 T4271 T4270 prep with,titrated
R2837 T4272 T4273 amod fresh,hydroxide
R2838 T4273 T4271 pobj hydroxide,with
R2839 T4274 T4273 compound ammonium,hydroxide
R2840 T4275 T4254 punct .,immersed
R2841 T4277 T4278 prep After,washed
R2842 T4279 T4280 nummod 20,min
R2843 T4280 T4277 pobj min,After
R2844 T4281 T4278 punct ", ",washed
R2845 T4282 T4278 nsubjpass slides,washed
R2846 T4283 T4278 auxpass were,washed
R2847 T4284 T4278 prep with,washed
R2848 T4285 T4286 compound ammonia,water
R2849 T4286 T4284 pobj water,with
R2850 T4287 T4278 prep before,washed
R2851 T4288 T4289 auxpass being,developed
R2852 T4289 T4287 pcomp developed,before
R2853 T4290 T4289 advmod individually,developed
R2854 T4291 T4289 prep with,developed
R2855 T4292 T4293 nummod 100,μl
R2856 T4293 T4291 pobj μl,with
R2857 T4294 T4293 prep of,μl
R2858 T4295 T4294 pobj developer,of
R2859 T4296 T4297 punct (,ml
R2860 T4297 T4295 parataxis ml,developer
R2861 T4298 T4297 nummod 20,ml
R2862 T4299 T4297 prep of,ml
R2863 T4300 T4301 nummod 37,%
R2864 T4301 T4302 compound %,formaldehyde
R2865 T4302 T4299 pobj formaldehyde,of
R2866 T4303 T4297 punct ", ",ml
R2867 T4304 T4305 nummod 100,ml
R2868 T4305 T4297 conj ml,ml
R2869 T4306 T4305 prep of,ml
R2870 T4307 T4308 amod distilled,water
R2871 T4308 T4306 pobj water,of
R2872 T4309 T4305 punct ", ",ml
R2873 T4310 T4311 nummod 50,μl
R2874 T4311 T4305 conj μl,ml
R2875 T4312 T4311 prep of,μl
R2876 T4313 T4314 amod concentrated,acid
R2877 T4314 T4312 pobj acid,of
R2878 T4315 T4314 amod nitric,acid
R2879 T4316 T4311 punct ", ",μl
R2880 T4317 T4311 cc and,μl
R2881 T4318 T4319 nummod 0.5,g
R2882 T4319 T4311 conj g,μl
R2883 T4320 T4319 prep of,g
R2884 T4321 T4322 amod citric,acid
R2885 T4322 T4320 pobj acid,of
R2886 T4323 T4297 punct ),ml
R2887 T4324 T4293 acl added,μl
R2888 T4325 T4324 prep to,added
R2889 T4326 T4327 nummod 50,ml
R2890 T4327 T4325 pobj ml,to
R2891 T4328 T4327 prep of,ml
R2892 T4329 T4330 amod titrated,solution
R2893 T4330 T4328 pobj solution,of
R2894 T4331 T4332 compound silver,nitrate
R2895 T4332 T4330 compound nitrate,solution
R2896 T4333 T4278 punct .,washed
R2897 T4335 T4336 nsubjpass Slides,rinsed
R2898 T4337 T4336 auxpass were,rinsed
R2899 T4338 T4336 advmod then,rinsed
R2900 T4339 T4336 prep in,rinsed
R2901 T4340 T4341 compound tap,water
R2902 T4341 T4339 pobj water,in
R2903 T4342 T4336 punct ", ",rinsed
R2904 T4343 T4336 conj fixed,rinsed
R2905 T4344 T4343 prep in,fixed
R2906 T4345 T4346 nummod 5,%
R2907 T4346 T4347 compound %,thiosulfate
R2908 T4347 T4344 pobj thiosulfate,in
R2909 T4348 T4347 compound sodium,thiosulfate
R2910 T4349 T4343 punct ", ",fixed
R2911 T4350 T4343 cc and,fixed
R2912 T4351 T4343 conj dehydrated,fixed
R2913 T4352 T4351 prep through,dehydrated
R2914 T4353 T4352 pobj alcohols,through
R2915 T4354 T4353 cc and,alcohols
R2916 T4355 T4353 conj xylene,alcohols
R2917 T4356 T4336 punct .,rinsed
R2918 T4358 T4359 compound Thioflavin,S
R2919 T4359 T4361 compound S,staining
R2920 T4360 T4359 punct -,S
R2921 T4363 T4364 prep Following,performed
R2922 T4365 T4363 pobj deparaffinization,Following
R2923 T4366 T4365 prep of,deparaffinization
R2924 T4367 T4366 pobj sections,of
R2925 T4368 T4365 prep through,deparaffinization
R2926 T4369 T4368 pobj xylene,through
R2927 T4370 T4369 cc and,xylene
R2928 T4371 T4369 conj alcohols,xylene
R2929 T4372 T4364 punct ", ",performed
R2930 T4373 T4374 compound amyloid,impregnation
R2931 T4374 T4364 nsubjpass impregnation,performed
R2932 T4375 T4374 prep with,impregnation
R2933 T4376 T4377 compound thioflavin,S
R2934 T4377 T4375 pobj S,with
R2935 T4378 T4377 punct -,S
R2936 T4379 T4364 auxpass was,performed
R2937 T4380 T4364 prep according,performed
R2938 T4381 T4380 prep to,according
R2939 T4382 T4383 det the,modification
R2940 T4383 T4381 pobj modification,to
R2941 T4384 T4383 compound Guntern,modification
R2942 T4385 T4383 prep of,modification
R2943 T4386 T4387 det the,protocol
R2944 T4387 T4385 pobj protocol,of
R2945 T4388 T4387 amod standard,protocol
R2946 T4389 T4364 punct .,performed
R2947 T4391 T4392 nsubjpass Slides,washed
R2948 T4393 T4391 acl holding,Slides
R2949 T4394 T4395 nummod 10,μm
R2950 T4395 T4397 compound μm,sections
R2951 T4396 T4395 punct -,μm
R2952 T4397 T4393 dobj sections,holding
R2953 T4398 T4397 compound paraffin,sections
R2954 T4399 T4392 auxpass were,washed
R2955 T4400 T4392 advmod twice,washed
R2956 T4401 T4392 prep in,washed
R2957 T4402 T4403 amod distilled,water
R2958 T4403 T4401 pobj water,in
R2959 T4404 T4392 punct ", ",washed
R2960 T4405 T4406 advmod then,immersed
R2961 T4406 T4392 dep immersed,washed
R2962 T4407 T4406 prep for,immersed
R2963 T4408 T4409 nummod 5,min
R2964 T4409 T4407 pobj min,for
R2965 T4410 T4406 prep in,immersed
R2966 T4411 T4412 det a,solution
R2967 T4412 T4410 pobj solution,in
R2968 T4413 T4414 nummod 0.25,%
R2969 T4414 T4412 compound %,solution
R2970 T4415 T4412 compound potassium,solution
R2971 T4416 T4412 compound permanganate,solution
R2972 T4417 T4406 punct ", ",immersed
R2973 T4418 T4406 advcl followed,immersed
R2974 T4419 T4418 agent by,followed
R2975 T4420 T4421 nummod 5,min
R2976 T4421 T4419 pobj min,by
R2977 T4422 T4421 prep in,min
R2978 T4423 T4424 det a,solution
R2979 T4424 T4422 pobj solution,in
R2980 T4425 T4426 nummod 1,%
R2981 T4426 T4427 nmod %,metabisulfate
R2982 T4427 T4424 nmod metabisulfate,solution
R2983 T4428 T4427 nmod potassium,metabisulfate
R2984 T4429 T4427 punct /,metabisulfate
R2985 T4430 T4431 nummod 1,%
R2986 T4431 T4432 nmod %,acid
R2987 T4432 T4427 appos acid,metabisulfate
R2988 T4433 T4432 amod oxalic,acid
R2989 T4434 T4392 punct .,washed
R2990 T4436 T4437 prep After,placed
R2991 T4438 T4439 det this,preparation
R2992 T4439 T4436 pobj preparation,After
R2993 T4440 T4437 punct ", ",placed
R2994 T4441 T4437 nsubjpass slides,placed
R2995 T4442 T4437 auxpass were,placed
R2996 T4443 T4437 prep into,placed
R2997 T4444 T4445 det a,solution
R2998 T4445 T4443 pobj solution,into
R2999 T4446 T4445 amod filtered,solution
R3000 T4447 T4445 amod aqueous,solution
R3001 T4448 T4449 nummod 0.02,%
R3002 T4449 T4445 compound %,solution
R3003 T4450 T4451 compound thioflavin,S
R3004 T4451 T4445 compound S,solution
R3005 T4452 T4451 punct -,S
R3006 T4453 T4454 punct (,Schmid
R3007 T4454 T4445 parataxis Schmid,solution
R3008 T4455 T4456 compound Chroma,Gesellschaft
R3009 T4456 T4454 compound Gesellschaft,Schmid
R3010 T4457 T4456 punct -,Gesellschaft
R3011 T4458 T4454 punct ", ",Schmid
R3012 T4459 T4454 npadvmod Kongen,Schmid
R3013 T4460 T4454 punct ", ",Schmid
R3014 T4461 T4454 npadvmod Germany,Schmid
R3015 T4462 T4454 punct ),Schmid
R3016 T4463 T4437 prep for,placed
R3017 T4464 T4465 nummod 8,min
R3018 T4465 T4463 pobj min,for
R3019 T4466 T4437 punct .,placed
R3020 T4468 T4469 amod Excess,stain
R3021 T4469 T4470 nsubjpass stain,removed
R3022 T4471 T4470 auxpass was,removed
R3023 T4472 T4470 agent by,removed
R3024 T4473 T4474 nummod two,rinses
R3025 T4474 T4472 pobj rinses,by
R3026 T4475 T4474 amod brief,rinses
R3027 T4476 T4474 prep in,rinses
R3028 T4477 T4478 nummod 80,%
R3029 T4478 T4479 compound %,ethanol
R3030 T4479 T4476 pobj ethanol,in
R3031 T4480 T4474 punct ", ",rinses
R3032 T4481 T4482 advmod then,two
R3033 T4482 T4474 appos two,rinses
R3034 T4483 T4482 prep in,two
R3035 T4484 T4485 amod distilled,water
R3036 T4485 T4483 pobj water,in
R3037 T4486 T4474 punct ", ",rinses
R3038 T4487 T4488 prep after,finished
R3039 T4488 T4474 relcl finished,rinses
R3040 T4489 T4487 pobj which,after
R3041 T4490 T4488 nsubjpass slides,finished
R3042 T4491 T4488 auxpass were,finished
R3043 T4492 T4488 prep in,finished
R3044 T4493 T4494 amod aqueous,medium
R3045 T4494 T4492 pobj medium,in
R3046 T4495 T4494 compound mounting,medium
R3047 T4496 T4488 prep for,finished
R3048 T4497 T4498 compound florescence,photomicrography
R3049 T4498 T4496 pobj photomicrography,for
R3050 T4499 T4470 punct .,removed
R3051 T4502 T4501 punct ", ",Ubiquitin
R3052 T4503 T4504 amod glial,protein
R3053 T4504 T4501 conj protein,Ubiquitin
R3054 T4505 T4504 amod fibrillary,protein
R3055 T4506 T4504 amod acidic,protein
R3056 T4507 T4504 punct ", ",protein
R3057 T4508 T4504 cc and,protein
R3058 T4509 T4510 compound Aβ,immunohistochemistry
R3059 T4510 T4504 conj immunohistochemistry,protein
R3060 T4512 T4513 amod Prior,to
R3061 T4513 T4514 prep to,deparaffinized
R3062 T4515 T4513 pobj immunostaining,to
R3063 T4516 T4514 punct ", ",deparaffinized
R3064 T4517 T4514 nsubjpass slides,deparaffinized
R3065 T4518 T4514 auxpass were,deparaffinized
R3066 T4519 T4514 prep by,deparaffinized
R3067 T4520 T4521 compound oven,heating
R3068 T4521 T4519 pobj heating,by
R3069 T4522 T4514 advcl followed,deparaffinized
R3070 T4523 T4522 agent by,followed
R3071 T4524 T4523 pobj immersion,by
R3072 T4525 T4524 prep in,immersion
R3073 T4526 T4525 pobj xylene,in
R3074 T4527 T4514 punct .,deparaffinized
R3075 T4529 T4530 prep After,quenched
R3076 T4531 T4529 pobj rehydration,After
R3077 T4532 T4531 prep through,rehydration
R3078 T4533 T4534 amod graded,alcohols
R3079 T4534 T4532 pobj alcohols,through
R3080 T4535 T4531 prep into,rehydration
R3081 T4536 T4537 compound tap,water
R3082 T4537 T4535 pobj water,into
R3083 T4538 T4530 punct ", ",quenched
R3084 T4539 T4540 amod endogenous,activity
R3085 T4540 T4530 nsubjpass activity,quenched
R3086 T4541 T4540 compound peroxidase,activity
R3087 T4542 T4530 auxpass was,quenched
R3088 T4543 T4530 agent by,quenched
R3089 T4544 T4543 pobj incubation,by
R3090 T4545 T4544 prep with,incubation
R3091 T4546 T4547 nummod 3,%
R3092 T4547 T4548 compound %,peroxide
R3093 T4548 T4545 pobj peroxide,with
R3094 T4549 T4548 compound hydrogen,peroxide
R3095 T4550 T4544 prep in,incubation
R3096 T4551 T4550 pobj methanol,in
R3097 T4552 T4530 punct .,quenched
R3098 T4554 T4555 nsubjpass Slides,microwaved
R3099 T4556 T4555 auxpass were,microwaved
R3100 T4557 T4555 prep for,microwaved
R3101 T4558 T4559 quantmod 5,7
R3102 T4559 T4561 nummod 7,min
R3103 T4560 T4559 punct –,7
R3104 T4561 T4557 pobj min,for
R3105 T4562 T4555 prep in,microwaved
R3106 T4563 T4562 pobj water,in
R3107 T4564 T4555 punct ", ",microwaved
R3108 T4565 T4555 dep cooled,microwaved
R3109 T4566 T4565 prep for,cooled
R3110 T4567 T4568 nummod 5,min
R3111 T4568 T4566 pobj min,for
R3112 T4569 T4555 punct ", ",microwaved
R3113 T4570 T4571 advmod then,washed
R3114 T4571 T4555 dep washed,microwaved
R3115 T4572 T4571 prep in,washed
R3116 T4573 T4572 pobj TBS,in
R3117 T4574 T4555 punct .,microwaved
R3118 T4576 T4577 amod Nonspecific,staining
R3119 T4577 T4578 nsubjpass staining,blocked
R3120 T4579 T4578 auxpass was,blocked
R3121 T4580 T4578 prep for,blocked
R3122 T4581 T4582 nummod 1,h
R3123 T4582 T4580 pobj h,for
R3124 T4583 T4578 prep with,blocked
R3125 T4584 T4585 nummod 3,%
R3126 T4585 T4586 nmod %,serum
R3127 T4586 T4583 pobj serum,with
R3128 T4587 T4586 amod normal,serum
R3129 T4588 T4586 compound goat,serum
R3130 T4589 T4586 cc and,serum
R3131 T4590 T4591 nummod 0.1,%
R3132 T4591 T4592 compound %,X
R3133 T4592 T4586 conj X,serum
R3134 T4593 T4592 compound Triton,X
R3135 T4594 T4592 punct -,X
R3136 T4595 T4592 nummod 100,X
R3137 T4596 T4586 prep in,serum
R3138 T4597 T4596 pobj TBS,in
R3139 T4598 T4578 punct .,blocked
R3140 T4600 T4601 nsubjpass Slides,placed
R3141 T4602 T4601 auxpass were,placed
R3142 T4603 T4601 advmod then,placed
R3143 T4604 T4601 prep into,placed
R3144 T4605 T4606 amod primary,antibody
R3145 T4606 T4604 pobj antibody,into
R3146 T4607 T4608 punct (,antibodies
R3147 T4608 T4606 parataxis antibodies,antibody
R3148 T4609 T4610 nmod rabbit,antibody
R3149 T4610 T4608 dep antibody,antibodies
R3150 T4611 T4610 amod anti-Aβ,antibody
R3151 T4612 T4610 nmod peptide,antibody
R3152 T4613 T4610 amod polyclonal,antibody
R3153 T4614 T4610 punct ", ",antibody
R3154 T4615 T4616 compound Zymed,Laboratories
R3155 T4616 T4610 npadvmod Laboratories,antibody
R3156 T4617 T4610 punct ", ",antibody
R3157 T4618 T4619 compound South,Francisco
R3158 T4619 T4610 npadvmod Francisco,antibody
R3159 T4620 T4619 compound San,Francisco
R3160 T4621 T4610 punct ", ",antibody
R3161 T4622 T4610 npadvmod California,antibody
R3162 T4623 T4610 punct ", ",antibody
R3163 T4624 T4625 compound United,States
R3164 T4625 T4610 npadvmod States,antibody
R3165 T4626 T4608 punct ;,antibodies
R3166 T4627 T4608 nmod rabbit,antibodies
R3167 T4628 T4627 amod anti-ubiquitin,rabbit
R3168 T4629 T4627 cc and,rabbit
R3169 T4630 T4627 conj rabbit,rabbit
R3170 T4631 T4630 amod anti–glial,rabbit
R3171 T4632 T4633 amod fibrillary,protein
R3172 T4633 T4608 nmod protein,antibodies
R3173 T4634 T4633 amod acidic,protein
R3174 T4635 T4633 punct (,protein
R3175 T4636 T4633 appos GFAP,protein
R3176 T4637 T4608 punct ),antibodies
R3177 T4638 T4608 amod polyclonal,antibodies
R3178 T4639 T4608 punct ", ",antibodies
R3179 T4640 T4608 npadvmod Dako,antibodies
R3180 T4641 T4608 punct ", ",antibodies
R3181 T4642 T4608 npadvmod Carpinteria,antibodies
R3182 T4643 T4608 punct ", ",antibodies
R3183 T4644 T4608 npadvmod California,antibodies
R3184 T4645 T4608 punct ", ",antibodies
R3185 T4646 T4647 compound United,States
R3186 T4647 T4608 npadvmod States,antibodies
R3187 T4648 T4608 punct ),antibodies
R3188 T4649 T4606 acl diluted,antibody
R3189 T4650 T4649 npadvmod 1,diluted
R3190 T4651 T4652 punct :,500
R3191 T4652 T4650 prep 500,1
R3192 T4653 T4649 prep in,diluted
R3193 T4654 T4653 pobj TBS,in
R3194 T4655 T4649 prep with,diluted
R3195 T4656 T4657 nummod 2,%
R3196 T4657 T4658 nmod %,serum
R3197 T4658 T4655 pobj serum,with
R3198 T4659 T4658 amod normal,serum
R3199 T4660 T4658 compound goat,serum
R3200 T4661 T4601 cc and,placed
R3201 T4662 T4601 conj incubated,placed
R3202 T4663 T4662 advmod overnight,incubated
R3203 T4664 T4662 prep at,incubated
R3204 T4665 T4666 compound room,temperature
R3205 T4666 T4664 pobj temperature,at
R3206 T4667 T4601 punct .,placed
R3207 T4669 T4670 prep After,incubated
R3208 T4671 T4672 auxpass being,washed
R3209 T4672 T4669 pcomp washed,After
R3210 T4673 T4672 prep of,washed
R3211 T4674 T4675 amod excess,antibody
R3212 T4675 T4673 pobj antibody,of
R3213 T4676 T4675 amod primary,antibody
R3214 T4677 T4672 prep with,washed
R3215 T4678 T4679 amod several,changes
R3216 T4679 T4677 pobj changes,with
R3217 T4680 T4679 prep of,changes
R3218 T4681 T4680 pobj TBS,of
R3219 T4682 T4670 punct ", ",incubated
R3220 T4683 T4670 nsubjpass slides,incubated
R3221 T4684 T4670 auxpass were,incubated
R3222 T4685 T4670 prep with,incubated
R3223 T4686 T4687 preconj either,system
R3224 T4687 T4685 pobj system,with
R3225 T4688 T4687 det the,system
R3226 T4689 T4690 nmod Vectastain,Elite
R3227 T4690 T4687 nmod Elite,system
R3228 T4691 T4687 amod anti-rabbit,system
R3229 T4692 T4687 amod secondary,system
R3230 T4693 T4694 punct (,Laboratories
R3231 T4694 T4687 parataxis Laboratories,system
R3232 T4695 T4694 amod anti-Aβ,Laboratories
R3233 T4696 T4694 punct ;,Laboratories
R3234 T4697 T4694 compound Vector,Laboratories
R3235 T4698 T4694 punct ", ",Laboratories
R3236 T4699 T4694 npadvmod Burlingame,Laboratories
R3237 T4700 T4694 punct ", ",Laboratories
R3238 T4701 T4694 npadvmod California,Laboratories
R3239 T4702 T4694 punct ", ",Laboratories
R3240 T4703 T4704 compound United,States
R3241 T4704 T4694 npadvmod States,Laboratories
R3242 T4705 T4694 punct ),Laboratories
R3243 T4706 T4687 cc or,system
R3244 T4707 T4708 nmod peroxidase,reagents
R3245 T4708 T4687 conj reagents,system
R3246 T4709 T4707 punct /,peroxidase
R3247 T4710 T4707 amod anti-peroxidase,peroxidase
R3248 T4711 T4712 punct (,Monoclonals
R3249 T4712 T4708 parataxis Monoclonals,reagents
R3250 T4713 T4712 amod anti-ubitquitin,Monoclonals
R3251 T4714 T4713 cc and,anti-ubitquitin
R3252 T4715 T4713 conj anti-GFAP,anti-ubitquitin
R3253 T4716 T4712 punct ;,Monoclonals
R3254 T4717 T4712 compound Sternberger,Monoclonals
R3255 T4718 T4712 punct ", ",Monoclonals
R3256 T4719 T4712 npadvmod Lutherville,Monoclonals
R3257 T4720 T4712 punct ", ",Monoclonals
R3258 T4721 T4712 npadvmod Maryland,Monoclonals
R3259 T4722 T4712 punct ", ",Monoclonals
R3260 T4723 T4724 compound United,States
R3261 T4724 T4712 npadvmod States,Monoclonals
R3262 T4725 T4712 punct ),Monoclonals
R3263 T4726 T4670 prep according,incubated
R3264 T4727 T4726 prep to,according
R3265 T4728 T4729 det the,manufacturers
R3266 T4729 T4730 poss manufacturers,directions
R3267 T4730 T4727 pobj directions,to
R3268 T4731 T4729 case ',manufacturers
R3269 T4732 T4670 punct .,incubated
R3270 T4734 T4735 compound Antibody,binding
R3271 T4735 T4736 nsubjpass binding,visualized
R3272 T4737 T4736 auxpass was,visualized
R3273 T4738 T4736 prep with,visualized
R3274 T4739 T4738 pobj diaminobenzidene,with
R3275 T4740 T4736 punct ", ",visualized
R3276 T4741 T4736 cc and,visualized
R3277 T4742 T4743 nsubjpass sections,counterstained
R3278 T4743 T4736 conj counterstained,visualized
R3279 T4744 T4743 auxpass were,counterstained
R3280 T4745 T4743 prep with,counterstained
R3281 T4746 T4745 pobj hematoxylin,with
R3282 T4747 T4743 punct .,counterstained