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PMC:116589 / 5570-7243 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1666 0-8 NNS denotes Plasmids
T1668 9-13 VBD denotes were
T1667 14-25 VBN denotes constructed
T1669 26-29 IN denotes for
T1670 30-37 VBG denotes driving
T1671 38-48 JJ denotes widespread
T1672 49-59 NN denotes expression
T1673 60-62 IN denotes of
T1674 63-67 DT denotes each
T1675 68-70 IN denotes of
T1676 71-74 DT denotes the
T1678 75-79 NN denotes gene
T1680 79-80 HYPH denotes -
T1679 80-85 VBN denotes based
T1681 86-97 JJ denotes fluorescent
T1682 98-105 NN denotes protein
T1683 106-107 -LRB- denotes (
T1684 107-109 NN denotes FP
T1685 109-110 -RRB- denotes )
T1677 111-120 NNS denotes reporters
T1686 121-124 CC denotes and
T1687 125-129 VBD denotes were
T1688 130-136 VBN denotes tested
T1689 137-139 IN denotes in
T1690 140-142 NN denotes ES
T1691 143-147 NN denotes cell
T1693 147-148 HYPH denotes -
T1692 148-156 JJ denotes mediated
T1694 157-168 NNS denotes transgenics
T1695 169-171 IN denotes as
T1696 172-181 VBN denotes described
T1697 182-192 RB denotes previously
T1698 193-194 -LRB- denotes [
T1699 194-195 CD denotes 2
T1700 195-196 -RRB- denotes ]
T1701 196-197 . denotes .
T1702 197-338 sentence denotes Transgenic ES cells exhibiting robust expression of ECFP or EYFP were obtained at the same frequency as we had previously obtained for EGFP.
T1703 198-208 JJ denotes Transgenic
T1705 209-211 NN denotes ES
T1704 212-217 NNS denotes cells
T1707 218-228 VBG denotes exhibiting
T1708 229-235 JJ denotes robust
T1709 236-246 NN denotes expression
T1710 247-249 IN denotes of
T1711 250-254 NN denotes ECFP
T1712 255-257 CC denotes or
T1713 258-262 NN denotes EYFP
T1714 263-267 VBD denotes were
T1706 268-276 VBN denotes obtained
T1715 277-279 IN denotes at
T1716 280-283 DT denotes the
T1718 284-288 JJ denotes same
T1717 289-298 NN denotes frequency
T1719 299-301 IN denotes as
T1721 302-304 PRP denotes we
T1722 305-308 VBD denotes had
T1723 309-319 RB denotes previously
T1720 320-328 VBN denotes obtained
T1724 329-332 IN denotes for
T1725 333-337 NN denotes EGFP
T1726 337-338 . denotes .
T1727 338-476 sentence denotes Cells expressing one or other of the two GFP variant reporters were clearly distinguishable within a mixed population (Fig. 1a,1b,1c,1d).
T1728 339-344 NNS denotes Cells
T1730 345-355 VBG denotes expressing
T1731 356-359 CD denotes one
T1733 360-362 CC denotes or
T1732 363-368 NN denotes other
T1734 369-371 IN denotes of
T1735 372-375 DT denotes the
T1737 376-379 CD denotes two
T1738 380-383 NN denotes GFP
T1739 384-391 JJ denotes variant
T1736 392-401 NNS denotes reporters
T1729 402-406 VBD denotes were
T1740 407-414 RB denotes clearly
T1741 415-430 JJ denotes distinguishable
T1742 431-437 IN denotes within
T1743 438-439 DT denotes a
T1745 440-445 VBN denotes mixed
T1744 446-456 NN denotes population
T1746 457-458 -LRB- denotes (
T1748 458-462 NN denotes Fig.
T1749 463-465 NN denotes 1a
T1750 465-466 , denotes ,
T1751 466-468 NN denotes 1b
T1752 468-469 , denotes ,
T1753 469-471 NN denotes 1c
T1754 471-472 , denotes ,
T1747 472-474 NN denotes 1d
T1755 474-475 -RRB- denotes )
T1756 475-476 . denotes .
T1757 476-668 sentence denotes Interestingly however we were unable to recover clones expressing DsRed1 at the same frequency as for the GFP variant reporters after electroporation and selection under identical conditions.
T1758 477-490 RB denotes Interestingly
T1760 491-498 RB denotes however
T1761 499-501 PRP denotes we
T1759 502-506 VBD denotes were
T1762 507-513 JJ denotes unable
T1763 514-516 TO denotes to
T1764 517-524 VB denotes recover
T1765 525-531 NNS denotes clones
T1766 532-542 VBG denotes expressing
T1767 543-549 NN denotes DsRed1
T1768 550-552 IN denotes at
T1769 553-556 DT denotes the
T1771 557-561 JJ denotes same
T1770 562-571 NN denotes frequency
T1772 572-574 IN denotes as
T1773 575-578 IN denotes for
T1774 579-582 DT denotes the
T1776 583-586 NN denotes GFP
T1777 587-594 JJ denotes variant
T1775 595-604 NNS denotes reporters
T1778 605-610 IN denotes after
T1779 611-626 NN denotes electroporation
T1780 627-630 CC denotes and
T1781 631-640 NN denotes selection
T1782 641-646 IN denotes under
T1783 647-656 JJ denotes identical
T1784 657-667 NNS denotes conditions
T1785 667-668 . denotes .
T1786 668-901 sentence denotes Indeed the DsRed1 expressing clones that we were able to recover did not retain their characteristic ES cell morphology, usually appearing smaller and more spherical (Fig. 1e and 1f), or occasionally spindle shaped (data not shown).
T1787 669-675 RB denotes Indeed
T1789 676-679 DT denotes the
T1791 680-686 NN denotes DsRed1
T1792 687-697 VBG denotes expressing
T1790 698-704 NNS denotes clones
T1793 705-709 WDT denotes that
T1795 710-712 PRP denotes we
T1794 713-717 VBD denotes were
T1796 718-722 JJ denotes able
T1797 723-725 TO denotes to
T1798 726-733 VB denotes recover
T1799 734-737 VBD denotes did
T1800 738-741 RB denotes not
T1788 742-748 VB denotes retain
T1801 749-754 PRP$ denotes their
T1803 755-769 JJ denotes characteristic
T1804 770-772 NN denotes ES
T1805 773-777 NN denotes cell
T1802 778-788 NN denotes morphology
T1806 788-790 , denotes ,
T1807 790-797 RB denotes usually
T1808 798-807 VBG denotes appearing
T1809 808-815 JJR denotes smaller
T1810 816-819 CC denotes and
T1811 820-824 RBR denotes more
T1812 825-834 JJ denotes spherical
T1813 835-836 -LRB- denotes (
T1815 836-840 NNP denotes Fig.
T1814 841-843 NN denotes 1e
T1816 844-847 CC denotes and
T1817 848-850 NN denotes 1f
T1818 850-851 -RRB- denotes )
T1819 851-853 , denotes ,
T1820 853-855 CC denotes or
T1821 856-868 RB denotes occasionally
T1823 869-876 NN denotes spindle
T1822 877-883 VBN denotes shaped
T1824 884-885 -LRB- denotes (
T1826 885-889 NNS denotes data
T1827 890-893 RB denotes not
T1825 894-899 VBN denotes shown
T1828 899-900 -RRB- denotes )
T1829 900-901 . denotes .
T1830 901-1044 sentence denotes Unlike the fluorescence produced by the GFP variants which was evenly distributed throughout the cells, the DsRed signal was never homogenous.
T1831 902-908 IN denotes Unlike
T1833 909-912 DT denotes the
T1834 913-925 NN denotes fluorescence
T1835 926-934 VBN denotes produced
T1836 935-937 IN denotes by
T1837 938-941 DT denotes the
T1839 942-945 NN denotes GFP
T1838 946-954 NNS denotes variants
T1840 955-960 WDT denotes which
T1842 961-964 VBD denotes was
T1843 965-971 RB denotes evenly
T1841 972-983 VBN denotes distributed
T1844 984-994 IN denotes throughout
T1845 995-998 DT denotes the
T1846 999-1004 NNS denotes cells
T1847 1004-1006 , denotes ,
T1848 1006-1009 DT denotes the
T1850 1010-1015 NNP denotes DsRed
T1849 1016-1022 NN denotes signal
T1832 1023-1026 VBD denotes was
T1851 1027-1032 RB denotes never
T1852 1033-1043 JJ denotes homogenous
T1853 1043-1044 . denotes .
T1854 1044-1143 sentence denotes Red fluorescence was often aggregated in what appeared to be perinuclear regions of the cytoplasm.
T1855 1045-1048 JJ denotes Red
T1856 1049-1061 NN denotes fluorescence
T1858 1062-1065 VBD denotes was
T1859 1066-1071 RB denotes often
T1857 1072-1082 VBN denotes aggregated
T1860 1083-1085 IN denotes in
T1861 1086-1090 WP denotes what
T1862 1091-1099 VBD denotes appeared
T1863 1100-1102 TO denotes to
T1864 1103-1105 VB denotes be
T1865 1106-1117 JJ denotes perinuclear
T1866 1118-1125 NNS denotes regions
T1867 1126-1128 IN denotes of
T1868 1129-1132 DT denotes the
T1869 1133-1142 NN denotes cytoplasm
T1870 1142-1143 . denotes .
T1871 1143-1320 sentence denotes Similar observations have been made by Furuta and colleagues when they used recombinant sindbis virus for the labeling of dendrites and axons within the CNS of adult mice [10].
T1872 1144-1151 JJ denotes Similar
T1873 1152-1164 NNS denotes observations
T1875 1165-1169 VBP denotes have
T1876 1170-1174 VBN denotes been
T1874 1175-1179 VBN denotes made
T1877 1180-1182 IN denotes by
T1878 1183-1189 NNP denotes Furuta
T1879 1190-1193 CC denotes and
T1880 1194-1204 NNS denotes colleagues
T1881 1205-1209 WRB denotes when
T1883 1210-1214 PRP denotes they
T1882 1215-1219 VBD denotes used
T1884 1220-1231 JJ denotes recombinant
T1886 1232-1239 NN denotes sindbis
T1885 1240-1245 NN denotes virus
T1887 1246-1249 IN denotes for
T1888 1250-1253 DT denotes the
T1889 1254-1262 NN denotes labeling
T1890 1263-1265 IN denotes of
T1891 1266-1275 NNS denotes dendrites
T1892 1276-1279 CC denotes and
T1893 1280-1285 NNS denotes axons
T1894 1286-1292 IN denotes within
T1895 1293-1296 DT denotes the
T1896 1297-1300 NN denotes CNS
T1897 1301-1303 IN denotes of
T1898 1304-1309 JJ denotes adult
T1899 1310-1314 NNS denotes mice
T1900 1315-1316 -LRB- denotes [
T1901 1316-1318 CD denotes 10
T1902 1318-1319 -RRB- denotes ]
T1903 1319-1320 . denotes .
T1904 1320-1509 sentence denotes In order to generate a DsRed variant that may be amenable to use in ES cells and mice, we recently introduced various sequences that direct nuclear localization to the DsRed coding region.
T1905 1321-1323 IN denotes In
T1907 1324-1329 NN denotes order
T1908 1330-1332 TO denotes to
T1909 1333-1341 VB denotes generate
T1910 1342-1343 DT denotes a
T1912 1344-1349 NNP denotes DsRed
T1911 1350-1357 NN denotes variant
T1913 1358-1362 WDT denotes that
T1915 1363-1366 MD denotes may
T1914 1367-1369 VB denotes be
T1916 1370-1378 JJ denotes amenable
T1917 1379-1381 IN denotes to
T1918 1382-1385 NN denotes use
T1919 1386-1388 IN denotes in
T1920 1389-1391 NN denotes ES
T1921 1392-1397 NNS denotes cells
T1922 1398-1401 CC denotes and
T1923 1402-1406 NNS denotes mice
T1924 1406-1408 , denotes ,
T1925 1408-1410 PRP denotes we
T1926 1411-1419 RB denotes recently
T1906 1420-1430 VBD denotes introduced
T1927 1431-1438 JJ denotes various
T1928 1439-1448 NNS denotes sequences
T1929 1449-1453 WDT denotes that
T1930 1454-1460 VBP denotes direct
T1931 1461-1468 JJ denotes nuclear
T1932 1469-1481 NN denotes localization
T1933 1482-1484 IN denotes to
T1934 1485-1488 DT denotes the
T1936 1489-1494 NNP denotes DsRed
T1937 1495-1501 VBG denotes coding
T1935 1502-1508 NN denotes region
T1938 1508-1509 . denotes .
T1939 1509-1673 sentence denotes However, again we were unable to generate ES cells or mice exhibiting robust widespread reporter activity (A.-K.H. and V.E. Papaioannou, unpublished observations).
T1940 1510-1517 RB denotes However
T1942 1517-1519 , denotes ,
T1943 1519-1524 RB denotes again
T1944 1525-1527 PRP denotes we
T1941 1528-1532 VBD denotes were
T1945 1533-1539 JJ denotes unable
T1946 1540-1542 TO denotes to
T1947 1543-1551 VB denotes generate
T1948 1552-1554 NN denotes ES
T1949 1555-1560 NNS denotes cells
T1950 1561-1563 CC denotes or
T1951 1564-1568 NNS denotes mice
T1952 1569-1579 VBG denotes exhibiting
T1953 1580-1586 JJ denotes robust
T1955 1587-1597 JJ denotes widespread
T1956 1598-1606 NN denotes reporter
T1954 1607-1615 NN denotes activity
T1957 1616-1617 -LRB- denotes (
T1959 1617-1619 NNP denotes A.
T1961 1619-1620 HYPH denotes -
T1960 1620-1624 NNP denotes K.H.
T1962 1625-1628 CC denotes and
T1963 1629-1633 NNP denotes V.E.
T1958 1634-1645 NNP denotes Papaioannou
T1964 1645-1647 , denotes ,
T1965 1647-1658 JJ denotes unpublished
T1966 1659-1671 NNS denotes observations
T1967 1671-1672 -RRB- denotes )
T1968 1672-1673 . denotes .
R1000 T1713 T1711 conj EYFP,ECFP
R1001 T1714 T1706 auxpass were,obtained
R1002 T1715 T1706 prep at,obtained
R1003 T1716 T1717 det the,frequency
R1004 T1717 T1715 pobj frequency,at
R1005 T1718 T1717 amod same,frequency
R1006 T1719 T1720 mark as,obtained
R1007 T1720 T1717 advcl obtained,frequency
R1008 T1721 T1720 nsubj we,obtained
R1009 T1722 T1720 aux had,obtained
R1010 T1723 T1720 advmod previously,obtained
R1011 T1724 T1720 prep for,obtained
R1012 T1725 T1724 pobj EGFP,for
R1013 T1726 T1706 punct .,obtained
R1014 T1728 T1729 nsubj Cells,were
R1015 T1730 T1728 acl expressing,Cells
R1016 T1731 T1732 nummod one,other
R1017 T1732 T1730 dobj other,expressing
R1018 T1733 T1732 cc or,other
R1019 T1734 T1732 prep of,other
R1020 T1735 T1736 det the,reporters
R1021 T1736 T1734 pobj reporters,of
R1022 T1737 T1736 nummod two,reporters
R1023 T1738 T1736 nmod GFP,reporters
R1024 T1739 T1738 amod variant,GFP
R1025 T1740 T1741 advmod clearly,distinguishable
R1026 T1741 T1729 acomp distinguishable,were
R1027 T1742 T1729 prep within,were
R1028 T1743 T1744 det a,population
R1029 T1744 T1742 pobj population,within
R1030 T1745 T1744 amod mixed,population
R1031 T1746 T1747 punct (,1d
R1032 T1747 T1729 parataxis 1d,were
R1033 T1748 T1747 nmod Fig.,1d
R1034 T1749 T1747 nmod 1a,1d
R1035 T1750 T1747 punct ",",1d
R1036 T1751 T1747 nmod 1b,1d
R1037 T1752 T1747 punct ",",1d
R1038 T1753 T1747 nmod 1c,1d
R1039 T1754 T1747 punct ",",1d
R1040 T1755 T1747 punct ),1d
R1041 T1756 T1729 punct .,were
R1042 T1758 T1759 advmod Interestingly,were
R1043 T1760 T1759 advmod however,were
R1044 T1761 T1759 nsubj we,were
R1045 T1762 T1759 acomp unable,were
R1046 T1763 T1764 aux to,recover
R1047 T1764 T1762 xcomp recover,unable
R1048 T1765 T1764 dobj clones,recover
R1049 T1766 T1765 acl expressing,clones
R1050 T1767 T1766 dobj DsRed1,expressing
R1051 T1768 T1766 prep at,expressing
R1052 T1769 T1770 det the,frequency
R1053 T1770 T1768 pobj frequency,at
R1054 T1771 T1770 amod same,frequency
R1055 T1772 T1770 prep as,frequency
R1056 T1773 T1772 prep for,as
R1057 T1774 T1775 det the,reporters
R1058 T1775 T1773 pobj reporters,for
R1059 T1776 T1775 nmod GFP,reporters
R1060 T1777 T1776 amod variant,GFP
R1061 T1778 T1759 prep after,were
R1062 T1779 T1778 pobj electroporation,after
R1063 T1780 T1779 cc and,electroporation
R1064 T1781 T1779 conj selection,electroporation
R1065 T1782 T1779 prep under,electroporation
R1066 T1783 T1784 amod identical,conditions
R1067 T1784 T1782 pobj conditions,under
R1068 T1785 T1759 punct .,were
R1069 T1787 T1788 advmod Indeed,retain
R1070 T1789 T1790 det the,clones
R1071 T1790 T1788 nsubj clones,retain
R1072 T1791 T1792 npadvmod DsRed1,expressing
R1073 T1792 T1790 amod expressing,clones
R1074 T1793 T1794 dep that,were
R1075 T1794 T1790 relcl were,clones
R1076 T1795 T1794 nsubj we,were
R1077 T1796 T1794 acomp able,were
R1078 T1797 T1798 aux to,recover
R1079 T1798 T1796 xcomp recover,able
R1080 T1799 T1788 aux did,retain
R1081 T1800 T1788 neg not,retain
R1082 T1801 T1802 poss their,morphology
R1083 T1802 T1788 dobj morphology,retain
R1084 T1803 T1802 amod characteristic,morphology
R1085 T1804 T1805 compound ES,cell
R1086 T1805 T1802 compound cell,morphology
R1087 T1806 T1788 punct ", ",retain
R1088 T1807 T1808 advmod usually,appearing
R1089 T1808 T1788 advcl appearing,retain
R1090 T1809 T1808 oprd smaller,appearing
R1091 T1810 T1809 cc and,smaller
R1092 T1811 T1812 advmod more,spherical
R1093 T1812 T1809 conj spherical,smaller
R1094 T1813 T1814 punct (,1e
R1095 T1814 T1809 parataxis 1e,smaller
R1096 T1815 T1814 compound Fig.,1e
R1097 T1816 T1814 cc and,1e
R1098 T1817 T1814 conj 1f,1e
R1099 T1818 T1814 punct ),1e
R1100 T1819 T1809 punct ", ",smaller
R1101 T1820 T1809 cc or,smaller
R1102 T1821 T1822 advmod occasionally,shaped
R1103 T1822 T1809 conj shaped,smaller
R1104 T1823 T1822 npadvmod spindle,shaped
R1105 T1824 T1825 punct (,shown
R1106 T1825 T1788 parataxis shown,retain
R1107 T1826 T1825 nsubj data,shown
R1108 T1827 T1825 neg not,shown
R1109 T1828 T1825 punct ),shown
R1110 T1829 T1788 punct .,retain
R1111 T1831 T1832 prep Unlike,was
R1112 T1833 T1834 det the,fluorescence
R1113 T1834 T1831 pobj fluorescence,Unlike
R1114 T1835 T1834 acl produced,fluorescence
R1115 T1836 T1835 agent by,produced
R1116 T1837 T1838 det the,variants
R1117 T1838 T1836 pobj variants,by
R1118 T1839 T1838 compound GFP,variants
R1119 T1840 T1841 dep which,distributed
R1120 T1841 T1834 relcl distributed,fluorescence
R1121 T1842 T1841 auxpass was,distributed
R1122 T1843 T1841 advmod evenly,distributed
R1123 T1844 T1841 prep throughout,distributed
R1124 T1845 T1846 det the,cells
R1125 T1846 T1844 pobj cells,throughout
R1126 T1847 T1832 punct ", ",was
R1127 T1848 T1849 det the,signal
R1128 T1849 T1832 nsubj signal,was
R1129 T1850 T1849 compound DsRed,signal
R1130 T1851 T1832 neg never,was
R1131 T1852 T1832 acomp homogenous,was
R1132 T1853 T1832 punct .,was
R1133 T1855 T1856 amod Red,fluorescence
R1134 T1856 T1857 nsubjpass fluorescence,aggregated
R1135 T1858 T1857 auxpass was,aggregated
R1136 T1859 T1857 advmod often,aggregated
R1137 T1860 T1857 prep in,aggregated
R1138 T1861 T1862 dep what,appeared
R1139 T1862 T1860 pcomp appeared,in
R1140 T1863 T1864 aux to,be
R1141 T1864 T1862 xcomp be,appeared
R1142 T1865 T1866 amod perinuclear,regions
R1143 T1866 T1864 attr regions,be
R1144 T1867 T1866 prep of,regions
R1145 T1868 T1869 det the,cytoplasm
R1146 T1869 T1867 pobj cytoplasm,of
R1147 T1870 T1857 punct .,aggregated
R1148 T1872 T1873 amod Similar,observations
R1149 T1873 T1874 nsubjpass observations,made
R1150 T1875 T1874 aux have,made
R1151 T1876 T1874 auxpass been,made
R1152 T1877 T1874 agent by,made
R1153 T1878 T1877 pobj Furuta,by
R1154 T1879 T1878 cc and,Furuta
R1155 T1880 T1878 conj colleagues,Furuta
R1156 T1881 T1882 advmod when,used
R1157 T1882 T1874 advcl used,made
R1158 T1883 T1882 nsubj they,used
R1159 T1884 T1885 amod recombinant,virus
R1160 T1885 T1882 dobj virus,used
R1161 T1886 T1885 compound sindbis,virus
R1162 T1887 T1882 prep for,used
R1163 T1888 T1889 det the,labeling
R1164 T1889 T1887 pobj labeling,for
R1165 T1890 T1889 prep of,labeling
R1166 T1891 T1890 pobj dendrites,of
R1167 T1892 T1891 cc and,dendrites
R1168 T1893 T1891 conj axons,dendrites
R1169 T1894 T1889 prep within,labeling
R1170 T1895 T1896 det the,CNS
R1171 T1896 T1894 pobj CNS,within
R1172 T1897 T1896 prep of,CNS
R1173 T1898 T1899 amod adult,mice
R1174 T1899 T1897 pobj mice,of
R1175 T1900 T1901 punct [,10
R1176 T1901 T1874 parataxis 10,made
R1177 T1902 T1901 punct ],10
R1178 T1903 T1874 punct .,made
R1179 T1905 T1906 prep In,introduced
R1180 T1907 T1905 pobj order,In
R1181 T1908 T1909 aux to,generate
R1182 T1909 T1907 acl generate,order
R1183 T1910 T1911 det a,variant
R1184 T1911 T1909 dobj variant,generate
R1185 T1912 T1911 compound DsRed,variant
R1186 T1913 T1914 dep that,be
R1187 T1914 T1911 relcl be,variant
R1188 T1915 T1914 aux may,be
R1189 T1916 T1914 acomp amenable,be
R1190 T1917 T1916 prep to,amenable
R1191 T1918 T1917 pobj use,to
R1192 T1919 T1918 prep in,use
R1193 T1920 T1921 compound ES,cells
R1194 T1921 T1919 pobj cells,in
R1195 T1922 T1921 cc and,cells
R1196 T1923 T1921 conj mice,cells
R1197 T1924 T1906 punct ", ",introduced
R1198 T1925 T1906 nsubj we,introduced
R1199 T1926 T1906 advmod recently,introduced
R1200 T1927 T1928 amod various,sequences
R1201 T1928 T1906 dobj sequences,introduced
R1202 T1929 T1930 dep that,direct
R1203 T1930 T1928 relcl direct,sequences
R1204 T1931 T1932 amod nuclear,localization
R1205 T1932 T1930 dobj localization,direct
R1206 T1933 T1906 prep to,introduced
R1207 T1934 T1935 det the,region
R1208 T1935 T1933 pobj region,to
R1209 T1936 T1935 nmod DsRed,region
R1210 T1937 T1935 amod coding,region
R1211 T1938 T1906 punct .,introduced
R1212 T1940 T1941 advmod However,were
R1213 T1942 T1941 punct ", ",were
R1214 T1943 T1941 advmod again,were
R1215 T1944 T1941 nsubj we,were
R1216 T1945 T1941 acomp unable,were
R1217 T1946 T1947 aux to,generate
R1218 T1947 T1945 xcomp generate,unable
R1219 T1948 T1949 compound ES,cells
R1220 T1949 T1947 dobj cells,generate
R1221 T1950 T1949 cc or,cells
R1222 T1951 T1949 conj mice,cells
R1223 T1952 T1949 acl exhibiting,cells
R1224 T1953 T1954 amod robust,activity
R1225 T1954 T1952 dobj activity,exhibiting
R1226 T1955 T1954 amod widespread,activity
R1227 T1956 T1954 compound reporter,activity
R1228 T1957 T1958 punct (,Papaioannou
R1229 T1958 T1941 meta Papaioannou,were
R1230 T1959 T1960 nmod A.,K.H.
R1231 T1960 T1958 nmod K.H.,Papaioannou
R1232 T1961 T1960 punct -,K.H.
R1233 T1962 T1960 cc and,K.H.
R1234 T1963 T1960 conj V.E.,K.H.
R1235 T1964 T1958 punct ", ",Papaioannou
R1236 T1965 T1966 amod unpublished,observations
R1237 T1966 T1958 dep observations,Papaioannou
R1238 T1967 T1958 punct ),Papaioannou
R1239 T1968 T1941 punct .,were
R956 T1666 T1667 nsubjpass Plasmids,constructed
R957 T1668 T1667 auxpass were,constructed
R958 T1669 T1667 prep for,constructed
R959 T1670 T1669 pcomp driving,for
R960 T1671 T1672 amod widespread,expression
R961 T1672 T1670 dobj expression,driving
R962 T1673 T1672 prep of,expression
R963 T1674 T1673 pobj each,of
R964 T1675 T1674 prep of,each
R965 T1676 T1677 det the,reporters
R966 T1677 T1675 pobj reporters,of
R967 T1678 T1679 npadvmod gene,based
R968 T1679 T1677 amod based,reporters
R969 T1680 T1679 punct -,based
R970 T1681 T1682 amod fluorescent,protein
R971 T1682 T1677 nmod protein,reporters
R972 T1683 T1684 punct (,FP
R973 T1684 T1682 appos FP,protein
R974 T1685 T1677 punct ),reporters
R975 T1686 T1667 cc and,constructed
R976 T1687 T1688 auxpass were,tested
R977 T1688 T1667 conj tested,constructed
R978 T1689 T1688 prep in,tested
R979 T1690 T1691 compound ES,cell
R980 T1691 T1692 npadvmod cell,mediated
R981 T1692 T1694 amod mediated,transgenics
R982 T1693 T1692 punct -,mediated
R983 T1694 T1689 pobj transgenics,in
R984 T1695 T1696 mark as,described
R985 T1696 T1688 advcl described,tested
R986 T1697 T1696 advmod previously,described
R987 T1698 T1699 punct [,2
R988 T1699 T1688 parataxis 2,tested
R989 T1700 T1699 punct ],2
R990 T1701 T1667 punct .,constructed
R991 T1703 T1704 amod Transgenic,cells
R992 T1704 T1706 nsubjpass cells,obtained
R993 T1705 T1704 compound ES,cells
R994 T1707 T1704 acl exhibiting,cells
R995 T1708 T1709 amod robust,expression
R996 T1709 T1707 dobj expression,exhibiting
R997 T1710 T1709 prep of,expression
R998 T1711 T1710 pobj ECFP,of
R999 T1712 T1711 cc or,ECFP