PMC:116589 / 4207-4818 5 Projects
Annnotations
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1399 | 0-2 | PRP | denotes | We |
| T1400 | 3-9 | VBD | denotes | tested |
| T1401 | 10-17 | JJ | denotes | several |
| T1402 | 18-20 | IN | denotes | of |
| T1403 | 21-24 | DT | denotes | the |
| T1405 | 25-34 | JJ | denotes | available |
| T1406 | 35-40 | NN | denotes | wtGFP |
| T1404 | 41-49 | NNS | denotes | variants |
| T1407 | 50-53 | IN | denotes | for |
| T1408 | 54-67 | JJ | denotes | developmental |
| T1409 | 68-78 | NN | denotes | neutrality |
| T1410 | 79-82 | CC | denotes | and |
| T1411 | 83-92 | NN | denotes | stability |
| T1412 | 93-95 | IN | denotes | of |
| T1413 | 96-106 | JJ | denotes | widespread |
| T1414 | 107-117 | NN | denotes | expression |
| T1415 | 117-118 | . | denotes | . |
| T1416 | 118-303 | sentence | denotes | An ES cell-mediated transgenic approach was used to evaluate the Enhanced Cyan Fluorescent Protein (ECFP) and Enhanced Yellow Fluorescent Protein (EYFP) wtGFP spectral variants [4,11]. |
| T1417 | 119-121 | DT | denotes | An |
| T1419 | 122-124 | NN | denotes | ES |
| T1420 | 125-129 | NN | denotes | cell |
| T1422 | 129-130 | HYPH | denotes | - |
| T1421 | 130-138 | VBN | denotes | mediated |
| T1423 | 139-149 | JJ | denotes | transgenic |
| T1418 | 150-158 | NN | denotes | approach |
| T1425 | 159-162 | VBD | denotes | was |
| T1424 | 163-167 | VBN | denotes | used |
| T1426 | 168-170 | TO | denotes | to |
| T1427 | 171-179 | VB | denotes | evaluate |
| T1428 | 180-183 | DT | denotes | the |
| T1430 | 184-192 | VBN | denotes | Enhanced |
| T1431 | 193-197 | NN | denotes | Cyan |
| T1432 | 198-209 | JJ | denotes | Fluorescent |
| T1429 | 210-217 | NN | denotes | Protein |
| T1434 | 218-219 | -LRB- | denotes | ( |
| T1435 | 219-223 | NN | denotes | ECFP |
| T1436 | 223-224 | -RRB- | denotes | ) |
| T1437 | 225-228 | CC | denotes | and |
| T1438 | 229-237 | VBD | denotes | Enhanced |
| T1440 | 238-244 | JJ | denotes | Yellow |
| T1441 | 245-256 | JJ | denotes | Fluorescent |
| T1439 | 257-264 | NN | denotes | Protein |
| T1442 | 265-266 | -LRB- | denotes | ( |
| T1443 | 266-270 | NN | denotes | EYFP |
| T1444 | 270-271 | -RRB- | denotes | ) |
| T1445 | 272-277 | NN | denotes | wtGFP |
| T1446 | 278-286 | JJ | denotes | spectral |
| T1433 | 287-295 | NNS | denotes | variants |
| T1447 | 296-297 | -LRB- | denotes | [ |
| T1449 | 297-298 | CD | denotes | 4 |
| T1450 | 298-299 | , | denotes | , |
| T1448 | 299-301 | CD | denotes | 11 |
| T1451 | 301-302 | -RRB- | denotes | ] |
| T1452 | 302-303 | . | denotes | . |
| T1453 | 303-456 | sentence | denotes | ECFP harbors six amino substitutions (Y66W, F64L, S65T, N146I, M153T, V163A) as compared to GFP, whereas EYFP harbors four (S65G, V68L, S72A and T203Y). |
| T1454 | 304-308 | NN | denotes | ECFP |
| T1455 | 309-316 | VBZ | denotes | harbors |
| T1456 | 317-320 | CD | denotes | six |
| T1458 | 321-326 | NN | denotes | amino |
| T1457 | 327-340 | NNS | denotes | substitutions |
| T1459 | 341-342 | -LRB- | denotes | ( |
| T1460 | 342-346 | NN | denotes | Y66W |
| T1462 | 346-348 | , | denotes | , |
| T1463 | 348-352 | NN | denotes | F64L |
| T1464 | 352-354 | , | denotes | , |
| T1465 | 354-358 | NN | denotes | S65T |
| T1466 | 358-360 | , | denotes | , |
| T1467 | 360-365 | NN | denotes | N146I |
| T1468 | 365-367 | , | denotes | , |
| T1469 | 367-372 | NN | denotes | M153T |
| T1470 | 372-374 | , | denotes | , |
| T1461 | 374-379 | NN | denotes | V163A |
| T1471 | 379-380 | -RRB- | denotes | ) |
| T1472 | 381-383 | IN | denotes | as |
| T1473 | 384-392 | VBN | denotes | compared |
| T1474 | 393-395 | IN | denotes | to |
| T1475 | 396-399 | NN | denotes | GFP |
| T1476 | 399-401 | , | denotes | , |
| T1477 | 401-408 | IN | denotes | whereas |
| T1479 | 409-413 | NN | denotes | EYFP |
| T1478 | 414-421 | VBZ | denotes | harbors |
| T1480 | 422-426 | CD | denotes | four |
| T1481 | 427-428 | -LRB- | denotes | ( |
| T1482 | 428-432 | NN | denotes | S65G |
| T1483 | 432-434 | , | denotes | , |
| T1484 | 434-438 | NN | denotes | V68L |
| T1485 | 438-440 | , | denotes | , |
| T1486 | 440-444 | NN | denotes | S72A |
| T1487 | 445-448 | CC | denotes | and |
| T1488 | 449-454 | NN | denotes | T203Y |
| T1489 | 454-455 | -RRB- | denotes | ) |
| T1490 | 455-456 | . | denotes | . |
| T1491 | 456-611 | sentence | denotes | In addition to increasing the quantum yield and solubility of the proteins these mutations shift the spectral profiles of the translation products [4,11]. |
| T1492 | 457-459 | IN | denotes | In |
| T1494 | 460-468 | NN | denotes | addition |
| T1495 | 469-471 | IN | denotes | to |
| T1496 | 472-482 | VBG | denotes | increasing |
| T1497 | 483-486 | DT | denotes | the |
| T1499 | 487-494 | NN | denotes | quantum |
| T1498 | 495-500 | NN | denotes | yield |
| T1500 | 501-504 | CC | denotes | and |
| T1501 | 505-515 | NN | denotes | solubility |
| T1502 | 516-518 | IN | denotes | of |
| T1503 | 519-522 | DT | denotes | the |
| T1504 | 523-531 | NN | denotes | proteins |
| T1505 | 532-537 | DT | denotes | these |
| T1506 | 538-547 | NNS | denotes | mutations |
| T1493 | 548-553 | VBP | denotes | shift |
| T1507 | 554-557 | DT | denotes | the |
| T1509 | 558-566 | JJ | denotes | spectral |
| T1508 | 567-575 | NNS | denotes | profiles |
| T1510 | 576-578 | IN | denotes | of |
| T1511 | 579-582 | DT | denotes | the |
| T1513 | 583-594 | NN | denotes | translation |
| T1512 | 595-603 | NNS | denotes | products |
| T1514 | 604-605 | -LRB- | denotes | [ |
| T1516 | 605-606 | CD | denotes | 4 |
| T1517 | 606-607 | , | denotes | , |
| T1515 | 607-609 | CD | denotes | 11 |
| T1518 | 609-610 | -RRB- | denotes | ] |
| T1519 | 610-611 | . | denotes | . |
| R705 | T1399 | T1400 | nsubj | We,tested |
| R706 | T1401 | T1400 | dobj | several,tested |
| R707 | T1402 | T1401 | prep | of,several |
| R708 | T1403 | T1404 | det | the,variants |
| R709 | T1404 | T1402 | pobj | variants,of |
| R710 | T1405 | T1404 | amod | available,variants |
| R711 | T1406 | T1404 | compound | wtGFP,variants |
| R712 | T1407 | T1400 | prep | for,tested |
| R713 | T1408 | T1409 | amod | developmental,neutrality |
| R714 | T1409 | T1407 | pobj | neutrality,for |
| R715 | T1410 | T1409 | cc | and,neutrality |
| R716 | T1411 | T1409 | conj | stability,neutrality |
| R717 | T1412 | T1411 | prep | of,stability |
| R718 | T1413 | T1414 | amod | widespread,expression |
| R719 | T1414 | T1412 | pobj | expression,of |
| R720 | T1415 | T1400 | punct | .,tested |
| R721 | T1417 | T1418 | det | An,approach |
| R722 | T1418 | T1424 | nsubjpass | approach,used |
| R723 | T1419 | T1418 | nmod | ES,approach |
| R724 | T1420 | T1421 | npadvmod | cell,mediated |
| R725 | T1421 | T1418 | amod | mediated,approach |
| R726 | T1422 | T1421 | punct | -,mediated |
| R727 | T1423 | T1418 | amod | transgenic,approach |
| R728 | T1425 | T1424 | auxpass | was,used |
| R729 | T1426 | T1427 | aux | to,evaluate |
| R730 | T1427 | T1424 | advcl | evaluate,used |
| R731 | T1428 | T1429 | det | the,Protein |
| R732 | T1429 | T1433 | nmod | Protein,variants |
| R733 | T1430 | T1429 | amod | Enhanced,Protein |
| R734 | T1431 | T1429 | nmod | Cyan,Protein |
| R735 | T1432 | T1429 | amod | Fluorescent,Protein |
| R736 | T1433 | T1427 | dobj | variants,evaluate |
| R737 | T1434 | T1429 | punct | (,Protein |
| R738 | T1435 | T1429 | appos | ECFP,Protein |
| R739 | T1436 | T1429 | punct | ),Protein |
| R740 | T1437 | T1429 | cc | and,Protein |
| R741 | T1438 | T1439 | nmod | Enhanced,Protein |
| R742 | T1439 | T1429 | conj | Protein,Protein |
| R743 | T1440 | T1439 | amod | Yellow,Protein |
| R744 | T1441 | T1439 | amod | Fluorescent,Protein |
| R745 | T1442 | T1439 | punct | (,Protein |
| R746 | T1443 | T1439 | appos | EYFP,Protein |
| R747 | T1444 | T1433 | punct | ),variants |
| R748 | T1445 | T1433 | nmod | wtGFP,variants |
| R749 | T1446 | T1433 | amod | spectral,variants |
| R750 | T1447 | T1448 | punct | [,11 |
| R751 | T1448 | T1424 | parataxis | 11,used |
| R752 | T1449 | T1448 | nummod | 4,11 |
| R753 | T1450 | T1448 | punct | ",",11 |
| R754 | T1451 | T1448 | punct | ],11 |
| R755 | T1452 | T1424 | punct | .,used |
| R756 | T1454 | T1455 | nsubj | ECFP,harbors |
| R757 | T1456 | T1457 | nummod | six,substitutions |
| R758 | T1457 | T1455 | dobj | substitutions,harbors |
| R759 | T1458 | T1457 | compound | amino,substitutions |
| R760 | T1459 | T1457 | punct | (,substitutions |
| R761 | T1460 | T1461 | nmod | Y66W,V163A |
| R762 | T1461 | T1457 | appos | V163A,substitutions |
| R763 | T1462 | T1461 | punct | ", ",V163A |
| R764 | T1463 | T1461 | nmod | F64L,V163A |
| R765 | T1464 | T1461 | punct | ", ",V163A |
| R766 | T1465 | T1461 | nmod | S65T,V163A |
| R767 | T1466 | T1461 | punct | ", ",V163A |
| R768 | T1467 | T1461 | nmod | N146I,V163A |
| R769 | T1468 | T1461 | punct | ", ",V163A |
| R770 | T1469 | T1461 | nmod | M153T,V163A |
| R771 | T1470 | T1461 | punct | ", ",V163A |
| R772 | T1471 | T1455 | punct | ),harbors |
| R773 | T1472 | T1455 | prep | as,harbors |
| R774 | T1473 | T1472 | pcomp | compared,as |
| R775 | T1474 | T1472 | prep | to,as |
| R776 | T1475 | T1474 | pobj | GFP,to |
| R777 | T1476 | T1455 | punct | ", ",harbors |
| R778 | T1477 | T1478 | mark | whereas,harbors |
| R779 | T1478 | T1455 | advcl | harbors,harbors |
| R780 | T1479 | T1478 | nsubj | EYFP,harbors |
| R781 | T1480 | T1478 | dobj | four,harbors |
| R782 | T1481 | T1480 | punct | (,four |
| R783 | T1482 | T1480 | appos | S65G,four |
| R784 | T1483 | T1482 | punct | ", ",S65G |
| R785 | T1484 | T1482 | conj | V68L,S65G |
| R786 | T1485 | T1484 | punct | ", ",V68L |
| R787 | T1486 | T1484 | conj | S72A,V68L |
| R788 | T1487 | T1486 | cc | and,S72A |
| R789 | T1488 | T1486 | conj | T203Y,S72A |
| R790 | T1489 | T1455 | punct | ),harbors |
| R791 | T1490 | T1455 | punct | .,harbors |
| R792 | T1492 | T1493 | prep | In,shift |
| R793 | T1494 | T1492 | pobj | addition,In |
| R794 | T1495 | T1494 | prep | to,addition |
| R795 | T1496 | T1495 | pcomp | increasing,to |
| R796 | T1497 | T1498 | det | the,yield |
| R797 | T1498 | T1496 | dobj | yield,increasing |
| R798 | T1499 | T1498 | compound | quantum,yield |
| R799 | T1500 | T1498 | cc | and,yield |
| R800 | T1501 | T1498 | conj | solubility,yield |
| R801 | T1502 | T1498 | prep | of,yield |
| R802 | T1503 | T1504 | det | the,proteins |
| R803 | T1504 | T1502 | pobj | proteins,of |
| R804 | T1505 | T1506 | det | these,mutations |
| R805 | T1506 | T1493 | nsubj | mutations,shift |
| R806 | T1507 | T1508 | det | the,profiles |
| R807 | T1508 | T1493 | dobj | profiles,shift |
| R808 | T1509 | T1508 | amod | spectral,profiles |
| R809 | T1510 | T1508 | prep | of,profiles |
| R810 | T1511 | T1512 | det | the,products |
| R811 | T1512 | T1510 | pobj | products,of |
| R812 | T1513 | T1512 | compound | translation,products |
| R813 | T1514 | T1515 | punct | [,11 |
| R814 | T1515 | T1493 | parataxis | 11,shift |
| R815 | T1516 | T1515 | nummod | 4,11 |
| R816 | T1517 | T1515 | punct | ",",11 |
| R817 | T1518 | T1515 | punct | ],11 |
| R818 | T1519 | T1493 | punct | .,shift |