| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T3227 |
0-6 |
NN |
denotes |
Vector |
| T3228 |
7-19 |
NN |
denotes |
construction |
| T3229 |
19-176 |
sentence |
denotes |
cDNAs encoding ECFP, EYFP and DsRed were amplified from plasmids pECFP, pEYFP and pDsRed1-N1 (Clontech, Inc.) by PCR using HiFi Taq Polymerase (Invitrogen). |
| T3230 |
20-25 |
NNS |
denotes |
cDNAs |
| T3232 |
26-34 |
VBG |
denotes |
encoding |
| T3233 |
35-39 |
NN |
denotes |
ECFP |
| T3234 |
39-41 |
, |
denotes |
, |
| T3235 |
41-45 |
NN |
denotes |
EYFP |
| T3236 |
46-49 |
CC |
denotes |
and |
| T3237 |
50-55 |
VBN |
denotes |
DsRed |
| T3238 |
56-60 |
VBD |
denotes |
were |
| T3239 |
61-70 |
VBN |
denotes |
amplified |
| T3240 |
71-75 |
IN |
denotes |
from |
| T3241 |
76-84 |
NNS |
denotes |
plasmids |
| T3242 |
85-90 |
NN |
denotes |
pECFP |
| T3243 |
90-92 |
, |
denotes |
, |
| T3244 |
92-97 |
NN |
denotes |
pEYFP |
| T3245 |
98-101 |
CC |
denotes |
and |
| T3246 |
102-109 |
NN |
denotes |
pDsRed1 |
| T3248 |
109-110 |
HYPH |
denotes |
- |
| T3247 |
110-112 |
NN |
denotes |
N1 |
| T3249 |
113-114 |
-LRB- |
denotes |
( |
| T3251 |
114-122 |
NNP |
denotes |
Clontech |
| T3252 |
122-124 |
, |
denotes |
, |
| T3250 |
124-128 |
NNP |
denotes |
Inc. |
| T3253 |
128-129 |
-RRB- |
denotes |
) |
| T3254 |
130-132 |
IN |
denotes |
by |
| T3255 |
133-136 |
NN |
denotes |
PCR |
| T3231 |
137-142 |
VBG |
denotes |
using |
| T3256 |
143-147 |
NNP |
denotes |
HiFi |
| T3258 |
148-151 |
NNP |
denotes |
Taq |
| T3257 |
152-162 |
NN |
denotes |
Polymerase |
| T3259 |
163-164 |
-LRB- |
denotes |
( |
| T3260 |
164-174 |
NN |
denotes |
Invitrogen |
| T3261 |
174-175 |
-RRB- |
denotes |
) |
| T3262 |
175-176 |
. |
denotes |
. |
| T3263 |
176-436 |
sentence |
denotes |
For ECFP and EYFP primers used were GFP-L (5'-TTG AAT TCG CCA CCA TGG TGA GC) and GFP-R (5'-TTG AAT TCT TAC TTG TAC AGC TCG TCC), for dsRed primers were red-L (5'-TTG AAT TCG CCA CCA TGG TGC GCT C) and red-R (5'-TTG AAT TCT TAC GCT ACA GCT ACA GGA ACA GGT G). |
| T3264 |
177-180 |
IN |
denotes |
For |
| T3266 |
181-185 |
NN |
denotes |
ECFP |
| T3267 |
186-189 |
CC |
denotes |
and |
| T3268 |
190-194 |
NN |
denotes |
EYFP |
| T3269 |
195-202 |
NNS |
denotes |
primers |
| T3270 |
203-207 |
VBN |
denotes |
used |
| T3265 |
208-212 |
VBD |
denotes |
were |
| T3272 |
213-216 |
NN |
denotes |
GFP |
| T3274 |
216-217 |
HYPH |
denotes |
- |
| T3273 |
217-218 |
NN |
denotes |
L |
| T3275 |
219-220 |
-LRB- |
denotes |
( |
| T3276 |
220-221 |
CD |
denotes |
5 |
| T3278 |
221-222 |
SYM |
denotes |
' |
| T3279 |
222-223 |
HYPH |
denotes |
- |
| T3280 |
223-226 |
NN |
denotes |
TTG |
| T3281 |
227-230 |
NN |
denotes |
AAT |
| T3282 |
231-234 |
NN |
denotes |
TCG |
| T3283 |
235-238 |
NN |
denotes |
CCA |
| T3284 |
239-242 |
NN |
denotes |
CCA |
| T3285 |
243-246 |
NN |
denotes |
TGG |
| T3286 |
247-250 |
NN |
denotes |
TGA |
| T3277 |
251-253 |
NN |
denotes |
GC |
| T3287 |
253-254 |
-RRB- |
denotes |
) |
| T3288 |
255-258 |
CC |
denotes |
and |
| T3289 |
259-262 |
NN |
denotes |
GFP |
| T3291 |
262-263 |
HYPH |
denotes |
- |
| T3290 |
263-264 |
NN |
denotes |
R |
| T3292 |
265-266 |
-LRB- |
denotes |
( |
| T3294 |
266-267 |
NN |
denotes |
5 |
| T3295 |
267-268 |
SYM |
denotes |
' |
| T3296 |
268-269 |
HYPH |
denotes |
- |
| T3297 |
269-272 |
NN |
denotes |
TTG |
| T3298 |
273-276 |
NN |
denotes |
AAT |
| T3299 |
277-280 |
NN |
denotes |
TCT |
| T3300 |
281-284 |
NN |
denotes |
TAC |
| T3301 |
285-288 |
NN |
denotes |
TTG |
| T3302 |
289-292 |
NN |
denotes |
TAC |
| T3303 |
293-296 |
NN |
denotes |
AGC |
| T3304 |
297-300 |
NN |
denotes |
TCG |
| T3293 |
301-304 |
NN |
denotes |
TCC |
| T3305 |
304-305 |
-RRB- |
denotes |
) |
| T3306 |
305-307 |
, |
denotes |
, |
| T3307 |
307-310 |
IN |
denotes |
for |
| T3308 |
311-316 |
JJ |
denotes |
dsRed |
| T3309 |
317-324 |
NNS |
denotes |
primers |
| T3271 |
325-329 |
VBD |
denotes |
were |
| T3310 |
330-333 |
NN |
denotes |
red |
| T3312 |
333-334 |
HYPH |
denotes |
- |
| T3311 |
334-335 |
NN |
denotes |
L |
| T3313 |
336-337 |
-LRB- |
denotes |
( |
| T3314 |
337-338 |
CD |
denotes |
5 |
| T3316 |
338-339 |
SYM |
denotes |
' |
| T3317 |
339-340 |
HYPH |
denotes |
- |
| T3318 |
340-343 |
NN |
denotes |
TTG |
| T3319 |
344-347 |
NN |
denotes |
AAT |
| T3320 |
348-351 |
NN |
denotes |
TCG |
| T3321 |
352-355 |
NN |
denotes |
CCA |
| T3322 |
356-359 |
NN |
denotes |
CCA |
| T3323 |
360-363 |
NN |
denotes |
TGG |
| T3324 |
364-367 |
NN |
denotes |
TGC |
| T3325 |
368-371 |
NN |
denotes |
GCT |
| T3315 |
372-373 |
NN |
denotes |
C |
| T3326 |
373-374 |
-RRB- |
denotes |
) |
| T3327 |
375-378 |
CC |
denotes |
and |
| T3328 |
379-382 |
NN |
denotes |
red |
| T3330 |
382-383 |
HYPH |
denotes |
- |
| T3329 |
383-384 |
NN |
denotes |
R |
| T3331 |
385-386 |
-LRB- |
denotes |
( |
| T3332 |
386-387 |
CD |
denotes |
5 |
| T3334 |
387-388 |
SYM |
denotes |
' |
| T3335 |
388-389 |
HYPH |
denotes |
- |
| T3336 |
389-392 |
NN |
denotes |
TTG |
| T3337 |
393-396 |
NN |
denotes |
AAT |
| T3338 |
397-400 |
NN |
denotes |
TCT |
| T3339 |
401-404 |
NN |
denotes |
TAC |
| T3340 |
405-408 |
NN |
denotes |
GCT |
| T3341 |
409-412 |
NN |
denotes |
ACA |
| T3342 |
413-416 |
NN |
denotes |
GCT |
| T3343 |
417-420 |
NN |
denotes |
ACA |
| T3344 |
421-424 |
NN |
denotes |
GGA |
| T3345 |
425-428 |
NN |
denotes |
ACA |
| T3346 |
429-432 |
NN |
denotes |
GGT |
| T3333 |
433-434 |
NN |
denotes |
G |
| T3347 |
434-435 |
-RRB- |
denotes |
) |
| T3348 |
435-436 |
. |
denotes |
. |
| T3349 |
436-588 |
sentence |
denotes |
All 5' primers contain a Kozak consensus translation initiation site upstream of the ATG for increased translation efficiency in eukaryotic cells [17]. |
| T3350 |
437-440 |
DT |
denotes |
All |
| T3352 |
441-442 |
CD |
denotes |
5 |
| T3353 |
442-443 |
SYM |
denotes |
' |
| T3351 |
444-451 |
NNS |
denotes |
primers |
| T3354 |
452-459 |
VBP |
denotes |
contain |
| T3355 |
460-461 |
DT |
denotes |
a |
| T3357 |
462-467 |
NNP |
denotes |
Kozak |
| T3358 |
468-477 |
NN |
denotes |
consensus |
| T3359 |
478-489 |
NN |
denotes |
translation |
| T3360 |
490-500 |
NN |
denotes |
initiation |
| T3356 |
501-505 |
NN |
denotes |
site |
| T3361 |
506-514 |
JJ |
denotes |
upstream |
| T3362 |
515-517 |
IN |
denotes |
of |
| T3363 |
518-521 |
DT |
denotes |
the |
| T3364 |
522-525 |
NN |
denotes |
ATG |
| T3365 |
526-529 |
IN |
denotes |
for |
| T3366 |
530-539 |
VBN |
denotes |
increased |
| T3368 |
540-551 |
NN |
denotes |
translation |
| T3367 |
552-562 |
NN |
denotes |
efficiency |
| T3369 |
563-565 |
IN |
denotes |
in |
| T3370 |
566-576 |
JJ |
denotes |
eukaryotic |
| T3371 |
577-582 |
NNS |
denotes |
cells |
| T3372 |
583-584 |
-LRB- |
denotes |
[ |
| T3373 |
584-586 |
CD |
denotes |
17 |
| T3374 |
586-587 |
-RRB- |
denotes |
] |
| T3375 |
587-588 |
. |
denotes |
. |
| T3376 |
588-815 |
sentence |
denotes |
To construct vectors driving ubiquitous transgene expression in ES cells and mice, the PCR amplified cDNAs were digested with EcoRI and inserted into the EcoRI sites of pCAGGS [18] to generate pCX-ECFP, pCX-EYFP and pCX-DsRed. |
| T3377 |
589-591 |
TO |
denotes |
To |
| T3378 |
592-601 |
VB |
denotes |
construct |
| T3380 |
602-609 |
NNS |
denotes |
vectors |
| T3381 |
610-617 |
VBG |
denotes |
driving |
| T3382 |
618-628 |
JJ |
denotes |
ubiquitous |
| T3384 |
629-638 |
NN |
denotes |
transgene |
| T3383 |
639-649 |
NN |
denotes |
expression |
| T3385 |
650-652 |
IN |
denotes |
in |
| T3386 |
653-655 |
NN |
denotes |
ES |
| T3387 |
656-661 |
NNS |
denotes |
cells |
| T3388 |
662-665 |
CC |
denotes |
and |
| T3389 |
666-670 |
NNS |
denotes |
mice |
| T3390 |
670-672 |
, |
denotes |
, |
| T3391 |
672-675 |
DT |
denotes |
the |
| T3393 |
676-679 |
NN |
denotes |
PCR |
| T3394 |
680-689 |
VBN |
denotes |
amplified |
| T3392 |
690-695 |
NNS |
denotes |
cDNAs |
| T3395 |
696-700 |
VBD |
denotes |
were |
| T3379 |
701-709 |
VBN |
denotes |
digested |
| T3396 |
710-714 |
IN |
denotes |
with |
| T3397 |
715-720 |
NN |
denotes |
EcoRI |
| T3398 |
721-724 |
CC |
denotes |
and |
| T3399 |
725-733 |
VBD |
denotes |
inserted |
| T3400 |
734-738 |
IN |
denotes |
into |
| T3401 |
739-742 |
DT |
denotes |
the |
| T3403 |
743-748 |
NN |
denotes |
EcoRI |
| T3402 |
749-754 |
NNS |
denotes |
sites |
| T3404 |
755-757 |
IN |
denotes |
of |
| T3405 |
758-764 |
NN |
denotes |
pCAGGS |
| T3406 |
765-766 |
-LRB- |
denotes |
[ |
| T3407 |
766-768 |
CD |
denotes |
18 |
| T3408 |
768-769 |
-RRB- |
denotes |
] |
| T3409 |
770-772 |
TO |
denotes |
to |
| T3410 |
773-781 |
VB |
denotes |
generate |
| T3411 |
782-785 |
NN |
denotes |
pCX |
| T3413 |
785-786 |
HYPH |
denotes |
- |
| T3412 |
786-790 |
NN |
denotes |
ECFP |
| T3414 |
790-792 |
, |
denotes |
, |
| T3415 |
792-795 |
NN |
denotes |
pCX |
| T3417 |
795-796 |
HYPH |
denotes |
- |
| T3416 |
796-800 |
NN |
denotes |
EYFP |
| T3418 |
801-804 |
CC |
denotes |
and |
| T3419 |
805-808 |
NN |
denotes |
pCX |
| T3421 |
808-809 |
HYPH |
denotes |
- |
| T3420 |
809-814 |
NN |
denotes |
DsRed |
| T3422 |
814-815 |
. |
denotes |
. |
| T3423 |
815-972 |
sentence |
denotes |
pCAGGS contains the chicken beta-actin promoter and first intron coupled to the CMV immediate early enhancer, and rabbit beta-globin polyadenylation signal. |
| T3424 |
816-822 |
NN |
denotes |
pCAGGS |
| T3425 |
823-831 |
VBZ |
denotes |
contains |
| T3426 |
832-835 |
DT |
denotes |
the |
| T3428 |
836-843 |
NN |
denotes |
chicken |
| T3429 |
844-848 |
NN |
denotes |
beta |
| T3431 |
848-849 |
HYPH |
denotes |
- |
| T3430 |
849-854 |
NN |
denotes |
actin |
| T3427 |
855-863 |
NN |
denotes |
promoter |
| T3432 |
864-867 |
CC |
denotes |
and |
| T3433 |
868-873 |
JJ |
denotes |
first |
| T3434 |
874-880 |
NN |
denotes |
intron |
| T3435 |
881-888 |
VBN |
denotes |
coupled |
| T3436 |
889-891 |
IN |
denotes |
to |
| T3437 |
892-895 |
DT |
denotes |
the |
| T3439 |
896-899 |
NN |
denotes |
CMV |
| T3440 |
900-909 |
JJ |
denotes |
immediate |
| T3441 |
910-915 |
JJ |
denotes |
early |
| T3438 |
916-924 |
NN |
denotes |
enhancer |
| T3442 |
924-926 |
, |
denotes |
, |
| T3443 |
926-929 |
CC |
denotes |
and |
| T3444 |
930-936 |
NN |
denotes |
rabbit |
| T3446 |
937-941 |
NN |
denotes |
beta |
| T3448 |
941-942 |
HYPH |
denotes |
- |
| T3447 |
942-948 |
NN |
denotes |
globin |
| T3449 |
949-964 |
NN |
denotes |
polyadenylation |
| T3445 |
965-971 |
NN |
denotes |
signal |
| T3450 |
971-972 |
. |
denotes |
. |
| T3451 |
972-1130 |
sentence |
denotes |
This promoter/enhancer combination has previously been shown to drive strong and widespread transgene expression in ES cells, embryos and adult mice [18,19]. |
| T3452 |
973-977 |
DT |
denotes |
This |
| T3454 |
978-986 |
NN |
denotes |
promoter |
| T3456 |
986-987 |
HYPH |
denotes |
/ |
| T3455 |
987-995 |
NN |
denotes |
enhancer |
| T3453 |
996-1007 |
NN |
denotes |
combination |
| T3458 |
1008-1011 |
VBZ |
denotes |
has |
| T3459 |
1012-1022 |
RB |
denotes |
previously |
| T3460 |
1023-1027 |
VBN |
denotes |
been |
| T3457 |
1028-1033 |
VBN |
denotes |
shown |
| T3461 |
1034-1036 |
TO |
denotes |
to |
| T3462 |
1037-1042 |
VB |
denotes |
drive |
| T3463 |
1043-1049 |
JJ |
denotes |
strong |
| T3465 |
1050-1053 |
CC |
denotes |
and |
| T3466 |
1054-1064 |
JJ |
denotes |
widespread |
| T3467 |
1065-1074 |
NN |
denotes |
transgene |
| T3464 |
1075-1085 |
NN |
denotes |
expression |
| T3468 |
1086-1088 |
IN |
denotes |
in |
| T3469 |
1089-1091 |
NN |
denotes |
ES |
| T3470 |
1092-1097 |
NNS |
denotes |
cells |
| T3471 |
1097-1099 |
, |
denotes |
, |
| T3472 |
1099-1106 |
NNS |
denotes |
embryos |
| T3473 |
1107-1110 |
CC |
denotes |
and |
| T3474 |
1111-1116 |
JJ |
denotes |
adult |
| T3475 |
1117-1121 |
NNS |
denotes |
mice |
| T3476 |
1122-1123 |
-LRB- |
denotes |
[ |
| T3478 |
1123-1125 |
CD |
denotes |
18 |
| T3479 |
1125-1126 |
, |
denotes |
, |
| T3477 |
1126-1128 |
CD |
denotes |
19 |
| T3480 |
1128-1129 |
-RRB- |
denotes |
] |
| T3481 |
1129-1130 |
. |
denotes |
. |
| R2188 |
T3227 |
T3228 |
compound |
Vector,construction |
| R2189 |
T3230 |
T3231 |
nsubjpass |
cDNAs,using |
| R2190 |
T3232 |
T3230 |
acl |
encoding,cDNAs |
| R2191 |
T3233 |
T3232 |
dobj |
ECFP,encoding |
| R2192 |
T3234 |
T3233 |
punct |
", ",ECFP |
| R2193 |
T3235 |
T3233 |
conj |
EYFP,ECFP |
| R2194 |
T3236 |
T3235 |
cc |
and,EYFP |
| R2195 |
T3237 |
T3235 |
conj |
DsRed,EYFP |
| R2196 |
T3238 |
T3231 |
auxpass |
were,using |
| R2197 |
T3239 |
T3231 |
aux |
amplified,using |
| R2198 |
T3240 |
T3231 |
prep |
from,using |
| R2199 |
T3241 |
T3242 |
compound |
plasmids,pECFP |
| R2200 |
T3242 |
T3240 |
pobj |
pECFP,from |
| R2201 |
T3243 |
T3242 |
punct |
", ",pECFP |
| R2202 |
T3244 |
T3242 |
conj |
pEYFP,pECFP |
| R2203 |
T3245 |
T3244 |
cc |
and,pEYFP |
| R2204 |
T3246 |
T3247 |
compound |
pDsRed1,N1 |
| R2205 |
T3247 |
T3244 |
conj |
N1,pEYFP |
| R2206 |
T3248 |
T3247 |
punct |
-,N1 |
| R2207 |
T3249 |
T3250 |
punct |
(,Inc. |
| R2208 |
T3250 |
T3242 |
parataxis |
Inc.,pECFP |
| R2209 |
T3251 |
T3250 |
nmod |
Clontech,Inc. |
| R2210 |
T3252 |
T3250 |
punct |
", ",Inc. |
| R2211 |
T3253 |
T3250 |
punct |
),Inc. |
| R2212 |
T3254 |
T3231 |
prep |
by,using |
| R2213 |
T3255 |
T3254 |
pobj |
PCR,by |
| R2214 |
T3256 |
T3257 |
compound |
HiFi,Polymerase |
| R2215 |
T3257 |
T3231 |
dobj |
Polymerase,using |
| R2216 |
T3258 |
T3257 |
compound |
Taq,Polymerase |
| R2217 |
T3259 |
T3260 |
punct |
(,Invitrogen |
| R2218 |
T3260 |
T3257 |
parataxis |
Invitrogen,Polymerase |
| R2219 |
T3261 |
T3260 |
punct |
),Invitrogen |
| R2220 |
T3262 |
T3231 |
punct |
.,using |
| R2221 |
T3264 |
T3265 |
prep |
For,were |
| R2222 |
T3265 |
T3271 |
ccomp |
were,were |
| R2223 |
T3266 |
T3264 |
pobj |
ECFP,For |
| R2224 |
T3267 |
T3266 |
cc |
and,ECFP |
| R2225 |
T3268 |
T3266 |
conj |
EYFP,ECFP |
| R2226 |
T3269 |
T3265 |
nsubj |
primers,were |
| R2227 |
T3270 |
T3269 |
acl |
used,primers |
| R2228 |
T3272 |
T3273 |
compound |
GFP,L |
| R2229 |
T3273 |
T3265 |
attr |
L,were |
| R2230 |
T3274 |
T3273 |
punct |
-,L |
| R2231 |
T3275 |
T3273 |
punct |
(,L |
| R2232 |
T3276 |
T3277 |
nummod |
5,GC |
| R2233 |
T3277 |
T3273 |
appos |
GC,L |
| R2234 |
T3278 |
T3276 |
punct |
',5 |
| R2235 |
T3279 |
T3277 |
punct |
-,GC |
| R2236 |
T3280 |
T3277 |
compound |
TTG,GC |
| R2237 |
T3281 |
T3277 |
compound |
AAT,GC |
| R2238 |
T3282 |
T3277 |
compound |
TCG,GC |
| R2239 |
T3283 |
T3277 |
compound |
CCA,GC |
| R2240 |
T3284 |
T3277 |
compound |
CCA,GC |
| R2241 |
T3285 |
T3277 |
compound |
TGG,GC |
| R2242 |
T3286 |
T3277 |
compound |
TGA,GC |
| R2243 |
T3287 |
T3273 |
punct |
),L |
| R2244 |
T3288 |
T3273 |
cc |
and,L |
| R2245 |
T3289 |
T3290 |
compound |
GFP,R |
| R2246 |
T3290 |
T3273 |
conj |
R,L |
| R2247 |
T3291 |
T3290 |
punct |
-,R |
| R2248 |
T3292 |
T3293 |
punct |
(,TCC |
| R2249 |
T3293 |
T3290 |
appos |
TCC,R |
| R2250 |
T3294 |
T3293 |
nmod |
5,TCC |
| R2251 |
T3295 |
T3294 |
punct |
',5 |
| R2252 |
T3296 |
T3293 |
punct |
-,TCC |
| R2253 |
T3297 |
T3293 |
compound |
TTG,TCC |
| R2254 |
T3298 |
T3293 |
compound |
AAT,TCC |
| R2255 |
T3299 |
T3293 |
compound |
TCT,TCC |
| R2256 |
T3300 |
T3293 |
compound |
TAC,TCC |
| R2257 |
T3301 |
T3293 |
compound |
TTG,TCC |
| R2258 |
T3302 |
T3293 |
compound |
TAC,TCC |
| R2259 |
T3303 |
T3293 |
compound |
AGC,TCC |
| R2260 |
T3304 |
T3293 |
compound |
TCG,TCC |
| R2261 |
T3305 |
T3271 |
punct |
),were |
| R2262 |
T3306 |
T3271 |
punct |
", ",were |
| R2263 |
T3307 |
T3271 |
prep |
for,were |
| R2264 |
T3308 |
T3307 |
pobj |
dsRed,for |
| R2265 |
T3309 |
T3271 |
nsubj |
primers,were |
| R2266 |
T3310 |
T3311 |
compound |
red,L |
| R2267 |
T3311 |
T3271 |
attr |
L,were |
| R2268 |
T3312 |
T3311 |
punct |
-,L |
| R2269 |
T3313 |
T3311 |
punct |
(,L |
| R2270 |
T3314 |
T3315 |
nummod |
5,C |
| R2271 |
T3315 |
T3311 |
appos |
C,L |
| R2272 |
T3316 |
T3314 |
punct |
',5 |
| R2273 |
T3317 |
T3315 |
punct |
-,C |
| R2274 |
T3318 |
T3315 |
compound |
TTG,C |
| R2275 |
T3319 |
T3315 |
compound |
AAT,C |
| R2276 |
T3320 |
T3315 |
compound |
TCG,C |
| R2277 |
T3321 |
T3315 |
compound |
CCA,C |
| R2278 |
T3322 |
T3315 |
compound |
CCA,C |
| R2279 |
T3323 |
T3315 |
compound |
TGG,C |
| R2280 |
T3324 |
T3315 |
compound |
TGC,C |
| R2281 |
T3325 |
T3315 |
compound |
GCT,C |
| R2282 |
T3326 |
T3311 |
punct |
),L |
| R2283 |
T3327 |
T3311 |
cc |
and,L |
| R2284 |
T3328 |
T3329 |
compound |
red,R |
| R2285 |
T3329 |
T3311 |
conj |
R,L |
| R2286 |
T3330 |
T3329 |
punct |
-,R |
| R2287 |
T3331 |
T3329 |
punct |
(,R |
| R2288 |
T3332 |
T3333 |
nummod |
5,G |
| R2289 |
T3333 |
T3329 |
appos |
G,R |
| R2290 |
T3334 |
T3332 |
punct |
',5 |
| R2291 |
T3335 |
T3333 |
punct |
-,G |
| R2292 |
T3336 |
T3333 |
compound |
TTG,G |
| R2293 |
T3337 |
T3333 |
compound |
AAT,G |
| R2294 |
T3338 |
T3333 |
compound |
TCT,G |
| R2295 |
T3339 |
T3333 |
compound |
TAC,G |
| R2296 |
T3340 |
T3333 |
compound |
GCT,G |
| R2297 |
T3341 |
T3333 |
compound |
ACA,G |
| R2298 |
T3342 |
T3333 |
compound |
GCT,G |
| R2299 |
T3343 |
T3333 |
compound |
ACA,G |
| R2300 |
T3344 |
T3333 |
compound |
GGA,G |
| R2301 |
T3345 |
T3333 |
compound |
ACA,G |
| R2302 |
T3346 |
T3333 |
compound |
GGT,G |
| R2303 |
T3347 |
T3271 |
punct |
),were |
| R2304 |
T3348 |
T3271 |
punct |
.,were |
| R2305 |
T3350 |
T3351 |
det |
All,primers |
| R2306 |
T3351 |
T3354 |
nsubj |
primers,contain |
| R2307 |
T3352 |
T3351 |
nummod |
5,primers |
| R2308 |
T3353 |
T3352 |
punct |
',5 |
| R2309 |
T3355 |
T3356 |
det |
a,site |
| R2310 |
T3356 |
T3354 |
dobj |
site,contain |
| R2311 |
T3357 |
T3358 |
compound |
Kozak,consensus |
| R2312 |
T3358 |
T3356 |
compound |
consensus,site |
| R2313 |
T3359 |
T3360 |
compound |
translation,initiation |
| R2314 |
T3360 |
T3356 |
compound |
initiation,site |
| R2315 |
T3361 |
T3356 |
amod |
upstream,site |
| R2316 |
T3362 |
T3361 |
prep |
of,upstream |
| R2317 |
T3363 |
T3364 |
det |
the,ATG |
| R2318 |
T3364 |
T3362 |
pobj |
ATG,of |
| R2319 |
T3365 |
T3354 |
prep |
for,contain |
| R2320 |
T3366 |
T3367 |
amod |
increased,efficiency |
| R2321 |
T3367 |
T3365 |
pobj |
efficiency,for |
| R2322 |
T3368 |
T3367 |
compound |
translation,efficiency |
| R2323 |
T3369 |
T3367 |
prep |
in,efficiency |
| R2324 |
T3370 |
T3371 |
amod |
eukaryotic,cells |
| R2325 |
T3371 |
T3369 |
pobj |
cells,in |
| R2326 |
T3372 |
T3373 |
punct |
[,17 |
| R2327 |
T3373 |
T3354 |
parataxis |
17,contain |
| R2328 |
T3374 |
T3373 |
punct |
],17 |
| R2329 |
T3375 |
T3354 |
punct |
.,contain |
| R2330 |
T3377 |
T3378 |
aux |
To,construct |
| R2331 |
T3378 |
T3379 |
advcl |
construct,digested |
| R2332 |
T3380 |
T3378 |
dobj |
vectors,construct |
| R2333 |
T3381 |
T3380 |
acl |
driving,vectors |
| R2334 |
T3382 |
T3383 |
amod |
ubiquitous,expression |
| R2335 |
T3383 |
T3381 |
dobj |
expression,driving |
| R2336 |
T3384 |
T3383 |
compound |
transgene,expression |
| R2337 |
T3385 |
T3381 |
prep |
in,driving |
| R2338 |
T3386 |
T3387 |
compound |
ES,cells |
| R2339 |
T3387 |
T3385 |
pobj |
cells,in |
| R2340 |
T3388 |
T3387 |
cc |
and,cells |
| R2341 |
T3389 |
T3387 |
conj |
mice,cells |
| R2342 |
T3390 |
T3379 |
punct |
", ",digested |
| R2343 |
T3391 |
T3392 |
det |
the,cDNAs |
| R2344 |
T3392 |
T3379 |
nsubjpass |
cDNAs,digested |
| R2345 |
T3393 |
T3394 |
npadvmod |
PCR,amplified |
| R2346 |
T3394 |
T3392 |
amod |
amplified,cDNAs |
| R2347 |
T3395 |
T3379 |
auxpass |
were,digested |
| R2348 |
T3396 |
T3379 |
prep |
with,digested |
| R2349 |
T3397 |
T3396 |
pobj |
EcoRI,with |
| R2350 |
T3398 |
T3379 |
cc |
and,digested |
| R2351 |
T3399 |
T3379 |
conj |
inserted,digested |
| R2352 |
T3400 |
T3399 |
prep |
into,inserted |
| R2353 |
T3401 |
T3402 |
det |
the,sites |
| R2354 |
T3402 |
T3400 |
pobj |
sites,into |
| R2355 |
T3403 |
T3402 |
compound |
EcoRI,sites |
| R2356 |
T3404 |
T3402 |
prep |
of,sites |
| R2357 |
T3405 |
T3404 |
pobj |
pCAGGS,of |
| R2358 |
T3406 |
T3407 |
punct |
[,18 |
| R2359 |
T3407 |
T3399 |
parataxis |
18,inserted |
| R2360 |
T3408 |
T3407 |
punct |
],18 |
| R2361 |
T3409 |
T3410 |
aux |
to,generate |
| R2362 |
T3410 |
T3399 |
advcl |
generate,inserted |
| R2363 |
T3411 |
T3412 |
compound |
pCX,ECFP |
| R2364 |
T3412 |
T3410 |
dobj |
ECFP,generate |
| R2365 |
T3413 |
T3412 |
punct |
-,ECFP |
| R2366 |
T3414 |
T3412 |
punct |
", ",ECFP |
| R2367 |
T3415 |
T3416 |
compound |
pCX,EYFP |
| R2368 |
T3416 |
T3412 |
conj |
EYFP,ECFP |
| R2369 |
T3417 |
T3416 |
punct |
-,EYFP |
| R2370 |
T3418 |
T3416 |
cc |
and,EYFP |
| R2371 |
T3419 |
T3420 |
compound |
pCX,DsRed |
| R2372 |
T3420 |
T3416 |
conj |
DsRed,EYFP |
| R2373 |
T3421 |
T3420 |
punct |
-,DsRed |
| R2374 |
T3422 |
T3379 |
punct |
.,digested |
| R2375 |
T3424 |
T3425 |
nsubj |
pCAGGS,contains |
| R2376 |
T3426 |
T3427 |
det |
the,promoter |
| R2377 |
T3427 |
T3425 |
dobj |
promoter,contains |
| R2378 |
T3428 |
T3427 |
compound |
chicken,promoter |
| R2379 |
T3429 |
T3430 |
compound |
beta,actin |
| R2380 |
T3430 |
T3427 |
compound |
actin,promoter |
| R2381 |
T3431 |
T3430 |
punct |
-,actin |
| R2382 |
T3432 |
T3427 |
cc |
and,promoter |
| R2383 |
T3433 |
T3434 |
amod |
first,intron |
| R2384 |
T3434 |
T3427 |
conj |
intron,promoter |
| R2385 |
T3435 |
T3427 |
acl |
coupled,promoter |
| R2386 |
T3436 |
T3435 |
prep |
to,coupled |
| R2387 |
T3437 |
T3438 |
det |
the,enhancer |
| R2388 |
T3438 |
T3436 |
pobj |
enhancer,to |
| R2389 |
T3439 |
T3438 |
nmod |
CMV,enhancer |
| R2390 |
T3440 |
T3438 |
amod |
immediate,enhancer |
| R2391 |
T3441 |
T3438 |
amod |
early,enhancer |
| R2392 |
T3442 |
T3427 |
punct |
", ",promoter |
| R2393 |
T3443 |
T3427 |
cc |
and,promoter |
| R2394 |
T3444 |
T3445 |
compound |
rabbit,signal |
| R2395 |
T3445 |
T3427 |
conj |
signal,promoter |
| R2396 |
T3446 |
T3447 |
compound |
beta,globin |
| R2397 |
T3447 |
T3445 |
compound |
globin,signal |
| R2398 |
T3448 |
T3447 |
punct |
-,globin |
| R2399 |
T3449 |
T3445 |
compound |
polyadenylation,signal |
| R2400 |
T3450 |
T3425 |
punct |
.,contains |
| R2401 |
T3452 |
T3453 |
det |
This,combination |
| R2402 |
T3453 |
T3457 |
nsubjpass |
combination,shown |
| R2403 |
T3454 |
T3455 |
compound |
promoter,enhancer |
| R2404 |
T3455 |
T3453 |
compound |
enhancer,combination |
| R2405 |
T3456 |
T3455 |
punct |
/,enhancer |
| R2406 |
T3458 |
T3457 |
aux |
has,shown |
| R2407 |
T3459 |
T3457 |
advmod |
previously,shown |
| R2408 |
T3460 |
T3457 |
auxpass |
been,shown |
| R2409 |
T3461 |
T3462 |
aux |
to,drive |
| R2410 |
T3462 |
T3457 |
xcomp |
drive,shown |
| R2411 |
T3463 |
T3464 |
amod |
strong,expression |
| R2412 |
T3464 |
T3462 |
dobj |
expression,drive |
| R2413 |
T3465 |
T3463 |
cc |
and,strong |
| R2414 |
T3466 |
T3463 |
conj |
widespread,strong |
| R2415 |
T3467 |
T3464 |
compound |
transgene,expression |
| R2416 |
T3468 |
T3462 |
prep |
in,drive |
| R2417 |
T3469 |
T3470 |
compound |
ES,cells |
| R2418 |
T3470 |
T3468 |
pobj |
cells,in |
| R2419 |
T3471 |
T3470 |
punct |
", ",cells |
| R2420 |
T3472 |
T3470 |
conj |
embryos,cells |
| R2421 |
T3473 |
T3472 |
cc |
and,embryos |
| R2422 |
T3474 |
T3475 |
amod |
adult,mice |
| R2423 |
T3475 |
T3472 |
conj |
mice,embryos |
| R2424 |
T3476 |
T3477 |
punct |
[,19 |
| R2425 |
T3477 |
T3457 |
parataxis |
19,shown |
| R2426 |
T3478 |
T3477 |
nummod |
18,19 |
| R2427 |
T3479 |
T3477 |
punct |
",",19 |
| R2428 |
T3480 |
T3477 |
punct |
],19 |
| R2429 |
T3481 |
T3457 |
punct |
.,shown |
