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PMC:1142324 / 3803-5993 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1243 0-10 NN denotes Generation
T1244 11-13 IN denotes of
T1245 14-20 NN denotes ADAM22
T1247 20-21 HYPH denotes -
T1246 21-30 JJ denotes deficient
T1248 31-35 NNS denotes mice
T1249 35-144 sentence denotes Mice carrying a targeted mutation in their Adam22 gene were generated by homologous recombination (Fig. 1A).
T1250 36-40 NNS denotes Mice
T1252 41-49 VBG denotes carrying
T1253 50-51 DT denotes a
T1255 52-60 VBN denotes targeted
T1254 61-69 NN denotes mutation
T1256 70-72 IN denotes in
T1257 73-78 PRP$ denotes their
T1259 79-85 NN denotes Adam22
T1258 86-90 NN denotes gene
T1260 91-95 VBD denotes were
T1251 96-105 VBN denotes generated
T1261 106-108 IN denotes by
T1262 109-119 JJ denotes homologous
T1263 120-133 NN denotes recombination
T1264 134-135 -LRB- denotes (
T1266 135-139 NN denotes Fig.
T1265 140-142 NN denotes 1A
T1267 142-143 -RRB- denotes )
T1268 143-144 . denotes .
T1269 144-221 sentence denotes Correct targeting events were confirmed by Southern blot analysis (Fig. 1B).
T1270 145-152 JJ denotes Correct
T1272 153-162 VBG denotes targeting
T1271 163-169 NNS denotes events
T1274 170-174 VBD denotes were
T1273 175-184 VBN denotes confirmed
T1275 185-187 IN denotes by
T1276 188-196 NNP denotes Southern
T1277 197-201 NN denotes blot
T1278 202-210 NN denotes analysis
T1279 211-212 -LRB- denotes (
T1281 212-216 NN denotes Fig.
T1280 217-219 NN denotes 1B
T1282 219-220 -RRB- denotes )
T1283 220-221 . denotes .
T1284 221-395 sentence denotes Since the termination codon was introduced in exon 8 in the pro-protein domain, only the truncated form of the ADAM22 protein would be synthesized from this targeted allele.
T1285 222-227 IN denotes Since
T1287 228-231 DT denotes the
T1289 232-243 NN denotes termination
T1288 244-249 NN denotes codon
T1290 250-253 VBD denotes was
T1286 254-264 VBN denotes introduced
T1292 265-267 IN denotes in
T1293 268-272 NN denotes exon
T1294 273-274 CD denotes 8
T1295 275-277 IN denotes in
T1296 278-281 DT denotes the
T1298 282-293 JJ denotes pro-protein
T1297 294-300 NN denotes domain
T1299 300-302 , denotes ,
T1300 302-306 RB denotes only
T1302 307-310 DT denotes the
T1303 311-320 VBN denotes truncated
T1301 321-325 NN denotes form
T1304 326-328 IN denotes of
T1305 329-332 DT denotes the
T1307 333-339 NN denotes ADAM22
T1306 340-347 NN denotes protein
T1308 348-353 MD denotes would
T1309 354-356 VB denotes be
T1291 357-368 VBN denotes synthesized
T1310 369-373 IN denotes from
T1311 374-378 DT denotes this
T1313 379-387 VBN denotes targeted
T1312 388-394 NN denotes allele
T1314 394-395 . denotes .
T1315 395-627 sentence denotes Because a pro-protein domain is always removed in the mature functional ADAM-proteins by the Furin-like proteases and is thought to be non-functional itself, we considered that this truncated form of ADAM22 protein has no function.
T1316 396-403 IN denotes Because
T1318 404-405 DT denotes a
T1320 406-417 JJ denotes pro-protein
T1319 418-424 NN denotes domain
T1321 425-427 VBZ denotes is
T1322 428-434 RB denotes always
T1317 435-442 VBN denotes removed
T1324 443-445 IN denotes in
T1325 446-449 DT denotes the
T1327 450-456 JJ denotes mature
T1328 457-467 JJ denotes functional
T1329 468-472 NN denotes ADAM
T1330 472-473 HYPH denotes -
T1326 473-481 NN denotes proteins
T1331 482-484 IN denotes by
T1332 485-488 DT denotes the
T1334 489-494 NN denotes Furin
T1336 494-495 HYPH denotes -
T1335 495-499 JJ denotes like
T1333 500-509 NNS denotes proteases
T1337 510-513 CC denotes and
T1338 514-516 VBZ denotes is
T1339 517-524 VBN denotes thought
T1340 525-527 TO denotes to
T1341 528-530 VB denotes be
T1342 531-545 JJ denotes non-functional
T1343 546-552 PRP denotes itself
T1344 552-554 , denotes ,
T1345 554-556 PRP denotes we
T1323 557-567 VBD denotes considered
T1346 568-572 IN denotes that
T1348 573-577 DT denotes this
T1350 578-587 VBN denotes truncated
T1349 588-592 NN denotes form
T1351 593-595 IN denotes of
T1352 596-602 NN denotes ADAM22
T1353 603-610 NN denotes protein
T1347 611-614 VBZ denotes has
T1354 615-617 DT denotes no
T1355 618-626 NN denotes function
T1356 626-627 . denotes .
T1357 627-823 sentence denotes Absence of mature ADAM22 protein in homozygous mutants was confirmed by Western blot analysis using the specific antibody, which recognizes the cytoplasmic domain of the ADAM22 protein (Fig. 1C).
T1358 628-635 NN denotes Absence
T1360 636-638 IN denotes of
T1361 639-645 JJ denotes mature
T1363 646-652 NN denotes ADAM22
T1362 653-660 NN denotes protein
T1364 661-663 IN denotes in
T1365 664-674 JJ denotes homozygous
T1366 675-682 NNS denotes mutants
T1367 683-686 VBD denotes was
T1359 687-696 VBN denotes confirmed
T1368 697-699 IN denotes by
T1369 700-707 NNP denotes Western
T1370 708-712 NN denotes blot
T1371 713-721 NN denotes analysis
T1372 722-727 VBG denotes using
T1373 728-731 DT denotes the
T1375 732-740 JJ denotes specific
T1374 741-749 NN denotes antibody
T1376 749-751 , denotes ,
T1377 751-756 WDT denotes which
T1378 757-767 VBZ denotes recognizes
T1379 768-771 DT denotes the
T1381 772-783 JJ denotes cytoplasmic
T1380 784-790 NN denotes domain
T1382 791-793 IN denotes of
T1383 794-797 DT denotes the
T1385 798-804 NN denotes ADAM22
T1384 805-812 NN denotes protein
T1386 813-814 -LRB- denotes (
T1388 814-818 NN denotes Fig.
T1387 819-821 NN denotes 1C
T1389 821-822 -RRB- denotes )
T1390 822-823 . denotes .
T1391 823-973 sentence denotes Homozygous mutants showed no noticeable defects at birth and were indistinguishable from wild-type or heterozygous littermates during the first week.
T1392 824-834 JJ denotes Homozygous
T1393 835-842 NNS denotes mutants
T1394 843-849 VBD denotes showed
T1395 850-852 DT denotes no
T1397 853-863 JJ denotes noticeable
T1396 864-871 NNS denotes defects
T1398 872-874 IN denotes at
T1399 875-880 NN denotes birth
T1400 881-884 CC denotes and
T1401 885-889 VBD denotes were
T1402 890-907 JJ denotes indistinguishable
T1403 908-912 IN denotes from
T1404 913-917 JJ denotes wild
T1406 917-918 HYPH denotes -
T1405 918-922 NN denotes type
T1408 923-925 CC denotes or
T1409 926-938 JJ denotes heterozygous
T1407 939-950 NNS denotes littermates
T1410 951-957 IN denotes during
T1411 958-961 DT denotes the
T1413 962-967 JJ denotes first
T1412 968-972 NN denotes week
T1414 972-973 . denotes .
T1415 973-1141 sentence denotes At postnatal day 10 (P10), most of the homozygous mutants were distinguishable by abnormalities such as reduced body weight and uncoordinated movements of their limbs.
T1416 974-976 IN denotes At
T1418 977-986 JJ denotes postnatal
T1419 987-990 NN denotes day
T1420 991-993 CD denotes 10
T1421 994-995 -LRB- denotes (
T1422 995-998 NN denotes P10
T1423 998-999 -RRB- denotes )
T1424 999-1001 , denotes ,
T1425 1001-1005 JJS denotes most
T1426 1006-1008 IN denotes of
T1427 1009-1012 DT denotes the
T1429 1013-1023 JJ denotes homozygous
T1428 1024-1031 NNS denotes mutants
T1417 1032-1036 VBD denotes were
T1430 1037-1052 JJ denotes distinguishable
T1431 1053-1055 IN denotes by
T1432 1056-1069 NNS denotes abnormalities
T1433 1070-1074 JJ denotes such
T1434 1075-1077 IN denotes as
T1435 1078-1085 VBN denotes reduced
T1437 1086-1090 NN denotes body
T1436 1091-1097 NN denotes weight
T1438 1098-1101 CC denotes and
T1439 1102-1115 JJ denotes uncoordinated
T1440 1116-1125 NNS denotes movements
T1441 1126-1128 IN denotes of
T1442 1129-1134 PRP$ denotes their
T1443 1135-1140 NNS denotes limbs
T1444 1140-1141 . denotes .
T1445 1141-1219 sentence denotes After P10, all homozygotes displayed severe ataxia (Fig. 2) and began to die.
T1446 1142-1147 IN denotes After
T1448 1148-1151 NN denotes P10
T1449 1151-1153 , denotes ,
T1450 1153-1156 DT denotes all
T1451 1157-1168 NNS denotes homozygotes
T1447 1169-1178 VBD denotes displayed
T1452 1179-1185 JJ denotes severe
T1453 1186-1192 NN denotes ataxia
T1454 1193-1194 -LRB- denotes (
T1455 1194-1198 NN denotes Fig.
T1456 1199-1200 CD denotes 2
T1457 1200-1201 -RRB- denotes )
T1458 1202-1205 CC denotes and
T1459 1206-1211 VBD denotes began
T1460 1212-1214 TO denotes to
T1461 1215-1218 VB denotes die
T1462 1218-1219 . denotes .
T1463 1219-1371 sentence denotes To measure the survival rate and body weight of each genotype precisely, we backcrossed heterozygous male mutants to C57BL/6 females more than 6 times.
T1464 1220-1222 TO denotes To
T1465 1223-1230 VB denotes measure
T1467 1231-1234 DT denotes the
T1469 1235-1243 NN denotes survival
T1468 1244-1248 NN denotes rate
T1470 1249-1252 CC denotes and
T1471 1253-1257 NN denotes body
T1472 1258-1264 NN denotes weight
T1473 1265-1267 IN denotes of
T1474 1268-1272 DT denotes each
T1475 1273-1281 NN denotes genotype
T1476 1282-1291 RB denotes precisely
T1477 1291-1293 , denotes ,
T1478 1293-1295 PRP denotes we
T1466 1296-1307 VBD denotes backcrossed
T1479 1308-1320 JJ denotes heterozygous
T1481 1321-1325 JJ denotes male
T1480 1326-1333 NNS denotes mutants
T1482 1334-1336 IN denotes to
T1483 1337-1342 NN denotes C57BL
T1485 1342-1343 HYPH denotes /
T1486 1343-1344 CD denotes 6
T1484 1345-1352 NNS denotes females
T1487 1353-1357 JJR denotes more
T1489 1358-1362 IN denotes than
T1488 1363-1364 CD denotes 6
T1490 1365-1370 NNS denotes times
T1491 1370-1371 . denotes .
T1492 1371-1482 sentence denotes The resulting (N 6) heterozygous males and females were intercrossed and the produced offspring were analysed.
T1493 1372-1375 DT denotes The
T1495 1376-1385 VBG denotes resulting
T1496 1386-1387 -LRB- denotes (
T1497 1387-1388 NN denotes N
T1498 1389-1390 CD denotes 6
T1499 1390-1391 -RRB- denotes )
T1500 1392-1404 JJ denotes heterozygous
T1494 1405-1410 NNS denotes males
T1502 1411-1414 CC denotes and
T1503 1415-1422 NNS denotes females
T1504 1423-1427 VBD denotes were
T1501 1428-1440 VBN denotes intercrossed
T1505 1441-1444 CC denotes and
T1506 1445-1448 DT denotes the
T1508 1449-1457 VBN denotes produced
T1507 1458-1467 NN denotes offspring
T1510 1468-1472 VBD denotes were
T1509 1473-1481 VBN denotes analysed
T1511 1481-1482 . denotes .
T1512 1482-1559 sentence denotes The numbers of survivors of each genotype every 5 days are shown in Table 1.
T1513 1483-1486 DT denotes The
T1514 1487-1494 NNS denotes numbers
T1516 1495-1497 IN denotes of
T1517 1498-1507 NNS denotes survivors
T1518 1508-1510 IN denotes of
T1519 1511-1515 DT denotes each
T1520 1516-1524 NN denotes genotype
T1521 1525-1530 DT denotes every
T1523 1531-1532 CD denotes 5
T1522 1533-1537 NNS denotes days
T1524 1538-1541 VBP denotes are
T1515 1542-1547 VBN denotes shown
T1525 1548-1550 IN denotes in
T1526 1551-1556 NN denotes Table
T1527 1557-1558 CD denotes 1
T1528 1558-1559 . denotes .
T1529 1559-1687 sentence denotes At P10, the ratio of each genotype was in close accordance with the Mendelian ratio (20.5% +/+, 55.1% +/-, 24.4% -/- ; n = 78).
T1530 1560-1562 IN denotes At
T1532 1563-1566 NN denotes P10
T1533 1566-1568 , denotes ,
T1534 1568-1571 DT denotes the
T1535 1572-1577 NN denotes ratio
T1536 1578-1580 IN denotes of
T1537 1581-1585 DT denotes each
T1538 1586-1594 NN denotes genotype
T1531 1595-1598 VBD denotes was
T1539 1599-1601 IN denotes in
T1540 1602-1607 JJ denotes close
T1541 1608-1618 NN denotes accordance
T1542 1619-1623 IN denotes with
T1543 1624-1627 DT denotes the
T1545 1628-1637 JJ denotes Mendelian
T1544 1638-1643 NN denotes ratio
T1546 1644-1645 -LRB- denotes (
T1548 1645-1649 CD denotes 20.5
T1547 1649-1650 NN denotes %
T1549 1651-1652 SYM denotes +
T1550 1652-1653 HYPH denotes /
T1551 1653-1654 SYM denotes +
T1552 1654-1656 , denotes ,
T1553 1656-1660 CD denotes 55.1
T1554 1660-1661 NN denotes %
T1555 1662-1663 SYM denotes +
T1556 1663-1664 HYPH denotes /
T1557 1664-1665 SYM denotes -
T1558 1665-1667 , denotes ,
T1559 1667-1671 CD denotes 24.4
T1560 1671-1672 NN denotes %
T1561 1673-1674 SYM denotes -
T1562 1674-1675 HYPH denotes /
T1563 1675-1676 SYM denotes -
T1564 1677-1678 : denotes ;
T1565 1679-1680 NN denotes n
T1567 1681-1682 SYM denotes =
T1566 1683-1685 CD denotes 78
T1568 1685-1686 -RRB- denotes )
T1569 1686-1687 . denotes .
T1570 1687-1753 sentence denotes This result shows that ADAM22 is not essential for embryogenesis.
T1571 1688-1692 DT denotes This
T1572 1693-1699 NN denotes result
T1573 1700-1705 VBZ denotes shows
T1574 1706-1710 IN denotes that
T1576 1711-1717 NN denotes ADAM22
T1575 1718-1720 VBZ denotes is
T1577 1721-1724 RB denotes not
T1578 1725-1734 JJ denotes essential
T1579 1735-1738 IN denotes for
T1580 1739-1752 NN denotes embryogenesis
T1581 1752-1753 . denotes .
T1582 1753-1888 sentence denotes At P10, the average body weight of homozygous mutants was approximately half that of wild-type and heterozygous littermates (Table 2).
T1583 1754-1756 IN denotes At
T1585 1757-1760 NN denotes P10
T1586 1760-1762 , denotes ,
T1587 1762-1765 DT denotes the
T1589 1766-1773 JJ denotes average
T1590 1774-1778 NN denotes body
T1588 1779-1785 NN denotes weight
T1591 1786-1788 IN denotes of
T1592 1789-1799 JJ denotes homozygous
T1593 1800-1807 NNS denotes mutants
T1584 1808-1811 VBD denotes was
T1594 1812-1825 RB denotes approximately
T1595 1826-1830 NN denotes half
T1596 1831-1835 DT denotes that
T1597 1836-1838 IN denotes of
T1598 1839-1843 JJ denotes wild
T1600 1843-1844 HYPH denotes -
T1599 1844-1848 NN denotes type
T1602 1849-1852 CC denotes and
T1603 1853-1865 JJ denotes heterozygous
T1601 1866-1877 NNS denotes littermates
T1604 1878-1879 -LRB- denotes (
T1605 1879-1884 NN denotes Table
T1606 1885-1886 CD denotes 2
T1607 1886-1887 -RRB- denotes )
T1608 1887-1888 . denotes .
T1609 1888-1971 sentence denotes Homozygous mutants died one by one after P10, and all homozygotes died before P20.
T1610 1889-1899 JJ denotes Homozygous
T1611 1900-1907 NNS denotes mutants
T1612 1908-1912 VBD denotes died
T1613 1913-1916 CD denotes one
T1614 1917-1919 IN denotes by
T1615 1920-1923 CD denotes one
T1616 1924-1929 IN denotes after
T1617 1930-1933 NN denotes P10
T1618 1933-1935 , denotes ,
T1619 1935-1938 CC denotes and
T1620 1939-1942 DT denotes all
T1621 1943-1954 NNS denotes homozygotes
T1622 1955-1959 VBD denotes died
T1623 1960-1966 IN denotes before
T1624 1967-1970 NN denotes P20
T1625 1970-1971 . denotes .
T1626 1971-2068 sentence denotes Of more than 100 homozygotes we have produced, none have survived more than 25 days after birth.
T1627 1972-1974 IN denotes Of
T1629 1975-1979 JJR denotes more
T1631 1980-1984 IN denotes than
T1630 1985-1988 CD denotes 100
T1632 1989-2000 NNS denotes homozygotes
T1633 2001-2003 PRP denotes we
T1635 2004-2008 VBP denotes have
T1634 2009-2017 VBN denotes produced
T1636 2017-2019 , denotes ,
T1637 2019-2023 NN denotes none
T1638 2024-2028 VBP denotes have
T1628 2029-2037 VBN denotes survived
T1639 2038-2042 JJR denotes more
T1641 2043-2047 IN denotes than
T1640 2048-2050 CD denotes 25
T1642 2051-2055 NNS denotes days
T1643 2056-2061 IN denotes after
T1644 2062-2067 NN denotes birth
T1645 2067-2068 . denotes .
T1646 2068-2190 sentence denotes Meanwhile, heterozygous mutants looked normal, were fertile, and survived for more than one year without obvious defects.
T1647 2069-2078 RB denotes Meanwhile
T1649 2078-2080 , denotes ,
T1650 2080-2092 JJ denotes heterozygous
T1651 2093-2100 NNS denotes mutants
T1648 2101-2107 VBD denotes looked
T1652 2108-2114 JJ denotes normal
T1653 2114-2116 , denotes ,
T1654 2116-2120 VBD denotes were
T1655 2121-2128 JJ denotes fertile
T1656 2128-2130 , denotes ,
T1657 2130-2133 CC denotes and
T1658 2134-2142 VBD denotes survived
T1659 2143-2146 IN denotes for
T1660 2147-2151 JJR denotes more
T1662 2152-2156 IN denotes than
T1661 2157-2160 CD denotes one
T1663 2161-2165 NN denotes year
T1664 2166-2173 IN denotes without
T1665 2174-2181 JJ denotes obvious
T1666 2182-2189 NNS denotes defects
T1667 2189-2190 . denotes .
R1000 T1605 T1584 parataxis Table,was
R1001 T1606 T1605 nummod 2,Table
R1002 T1607 T1605 punct ),Table
R1003 T1608 T1584 punct .,was
R1004 T1610 T1611 amod Homozygous,mutants
R1005 T1611 T1612 nsubj mutants,died
R1006 T1613 T1612 npadvmod one,died
R1007 T1614 T1613 prep by,one
R1008 T1615 T1614 pobj one,by
R1009 T1616 T1612 prep after,died
R1010 T1617 T1616 pobj P10,after
R1011 T1618 T1612 punct ", ",died
R1012 T1619 T1612 cc and,died
R1013 T1620 T1621 det all,homozygotes
R1014 T1621 T1622 nsubj homozygotes,died
R1015 T1622 T1612 conj died,died
R1016 T1623 T1622 prep before,died
R1017 T1624 T1623 pobj P20,before
R1018 T1625 T1622 punct .,died
R1019 T1627 T1628 prep Of,survived
R1020 T1629 T1630 amod more,100
R1021 T1630 T1632 nummod 100,homozygotes
R1022 T1631 T1630 quantmod than,100
R1023 T1632 T1627 pobj homozygotes,Of
R1024 T1633 T1634 nsubj we,produced
R1025 T1634 T1632 advcl produced,homozygotes
R1026 T1635 T1634 aux have,produced
R1027 T1636 T1628 punct ", ",survived
R1028 T1637 T1628 nsubj none,survived
R1029 T1638 T1628 aux have,survived
R1030 T1639 T1640 amod more,25
R1031 T1640 T1642 nummod 25,days
R1032 T1641 T1640 quantmod than,25
R1033 T1642 T1628 npadvmod days,survived
R1034 T1643 T1642 prep after,days
R1035 T1644 T1643 pobj birth,after
R1036 T1645 T1628 punct .,survived
R1037 T1647 T1648 advmod Meanwhile,looked
R1038 T1649 T1648 punct ", ",looked
R1039 T1650 T1651 amod heterozygous,mutants
R1040 T1651 T1648 nsubj mutants,looked
R1041 T1652 T1648 acomp normal,looked
R1042 T1653 T1648 punct ", ",looked
R1043 T1654 T1648 conj were,looked
R1044 T1655 T1654 acomp fertile,were
R1045 T1656 T1654 punct ", ",were
R1046 T1657 T1654 cc and,were
R1047 T1658 T1654 conj survived,were
R1048 T1659 T1658 prep for,survived
R1049 T1660 T1661 amod more,one
R1050 T1661 T1663 nummod one,year
R1051 T1662 T1661 quantmod than,one
R1052 T1663 T1659 pobj year,for
R1053 T1664 T1658 prep without,survived
R1054 T1665 T1666 amod obvious,defects
R1055 T1666 T1664 pobj defects,without
R1056 T1667 T1648 punct .,looked
R667 T1244 T1243 prep of,Generation
R668 T1245 T1246 npadvmod ADAM22,deficient
R669 T1246 T1248 amod deficient,mice
R670 T1247 T1246 punct -,deficient
R671 T1248 T1244 pobj mice,of
R672 T1250 T1251 nsubjpass Mice,generated
R673 T1252 T1250 acl carrying,Mice
R674 T1253 T1254 det a,mutation
R675 T1254 T1252 dobj mutation,carrying
R676 T1255 T1254 amod targeted,mutation
R677 T1256 T1252 prep in,carrying
R678 T1257 T1258 poss their,gene
R679 T1258 T1256 pobj gene,in
R680 T1259 T1258 compound Adam22,gene
R681 T1260 T1251 auxpass were,generated
R682 T1261 T1251 prep by,generated
R683 T1262 T1263 amod homologous,recombination
R684 T1263 T1261 pobj recombination,by
R685 T1264 T1265 punct (,1A
R686 T1265 T1251 parataxis 1A,generated
R687 T1266 T1265 compound Fig.,1A
R688 T1267 T1265 punct ),1A
R689 T1268 T1251 punct .,generated
R690 T1270 T1271 amod Correct,events
R691 T1271 T1273 nsubjpass events,confirmed
R692 T1272 T1271 amod targeting,events
R693 T1274 T1273 auxpass were,confirmed
R694 T1275 T1273 prep by,confirmed
R695 T1276 T1277 compound Southern,blot
R696 T1277 T1278 compound blot,analysis
R697 T1278 T1275 pobj analysis,by
R698 T1279 T1280 punct (,1B
R699 T1280 T1273 parataxis 1B,confirmed
R700 T1281 T1280 compound Fig.,1B
R701 T1282 T1280 punct ),1B
R702 T1283 T1273 punct .,confirmed
R703 T1285 T1286 mark Since,introduced
R704 T1286 T1291 advcl introduced,synthesized
R705 T1287 T1288 det the,codon
R706 T1288 T1286 nsubjpass codon,introduced
R707 T1289 T1288 compound termination,codon
R708 T1290 T1286 auxpass was,introduced
R709 T1292 T1286 prep in,introduced
R710 T1293 T1292 pobj exon,in
R711 T1294 T1293 nummod 8,exon
R712 T1295 T1286 prep in,introduced
R713 T1296 T1297 det the,domain
R714 T1297 T1295 pobj domain,in
R715 T1298 T1297 amod pro-protein,domain
R716 T1299 T1291 punct ", ",synthesized
R717 T1300 T1301 advmod only,form
R718 T1301 T1291 nsubjpass form,synthesized
R719 T1302 T1301 det the,form
R720 T1303 T1301 amod truncated,form
R721 T1304 T1301 prep of,form
R722 T1305 T1306 det the,protein
R723 T1306 T1304 pobj protein,of
R724 T1307 T1306 compound ADAM22,protein
R725 T1308 T1291 aux would,synthesized
R726 T1309 T1291 auxpass be,synthesized
R727 T1310 T1291 prep from,synthesized
R728 T1311 T1312 det this,allele
R729 T1312 T1310 pobj allele,from
R730 T1313 T1312 amod targeted,allele
R731 T1314 T1291 punct .,synthesized
R732 T1316 T1317 mark Because,removed
R733 T1317 T1323 advcl removed,considered
R734 T1318 T1319 det a,domain
R735 T1319 T1317 nsubjpass domain,removed
R736 T1320 T1319 amod pro-protein,domain
R737 T1321 T1317 auxpass is,removed
R738 T1322 T1317 advmod always,removed
R739 T1324 T1317 prep in,removed
R740 T1325 T1326 det the,proteins
R741 T1326 T1324 pobj proteins,in
R742 T1327 T1326 amod mature,proteins
R743 T1328 T1326 amod functional,proteins
R744 T1329 T1326 compound ADAM,proteins
R745 T1330 T1326 punct -,proteins
R746 T1331 T1317 agent by,removed
R747 T1332 T1333 det the,proteases
R748 T1333 T1331 pobj proteases,by
R749 T1334 T1335 npadvmod Furin,like
R750 T1335 T1333 amod like,proteases
R751 T1336 T1335 punct -,like
R752 T1337 T1317 cc and,removed
R753 T1338 T1339 auxpass is,thought
R754 T1339 T1317 conj thought,removed
R755 T1340 T1341 aux to,be
R756 T1341 T1339 xcomp be,thought
R757 T1342 T1341 acomp non-functional,be
R758 T1343 T1341 npadvmod itself,be
R759 T1344 T1323 punct ", ",considered
R760 T1345 T1323 nsubj we,considered
R761 T1346 T1347 mark that,has
R762 T1347 T1323 ccomp has,considered
R763 T1348 T1349 det this,form
R764 T1349 T1347 nsubj form,has
R765 T1350 T1349 amod truncated,form
R766 T1351 T1349 prep of,form
R767 T1352 T1353 compound ADAM22,protein
R768 T1353 T1351 pobj protein,of
R769 T1354 T1355 det no,function
R770 T1355 T1347 dobj function,has
R771 T1356 T1323 punct .,considered
R772 T1358 T1359 nsubjpass Absence,confirmed
R773 T1360 T1358 prep of,Absence
R774 T1361 T1362 amod mature,protein
R775 T1362 T1360 pobj protein,of
R776 T1363 T1362 compound ADAM22,protein
R777 T1364 T1358 prep in,Absence
R778 T1365 T1366 amod homozygous,mutants
R779 T1366 T1364 pobj mutants,in
R780 T1367 T1359 auxpass was,confirmed
R781 T1368 T1359 prep by,confirmed
R782 T1369 T1370 compound Western,blot
R783 T1370 T1371 compound blot,analysis
R784 T1371 T1368 pobj analysis,by
R785 T1372 T1371 acl using,analysis
R786 T1373 T1374 det the,antibody
R787 T1374 T1372 dobj antibody,using
R788 T1375 T1374 amod specific,antibody
R789 T1376 T1374 punct ", ",antibody
R790 T1377 T1378 dep which,recognizes
R791 T1378 T1374 relcl recognizes,antibody
R792 T1379 T1380 det the,domain
R793 T1380 T1378 dobj domain,recognizes
R794 T1381 T1380 amod cytoplasmic,domain
R795 T1382 T1380 prep of,domain
R796 T1383 T1384 det the,protein
R797 T1384 T1382 pobj protein,of
R798 T1385 T1384 compound ADAM22,protein
R799 T1386 T1387 punct (,1C
R800 T1387 T1359 parataxis 1C,confirmed
R801 T1388 T1387 compound Fig.,1C
R802 T1389 T1387 punct ),1C
R803 T1390 T1359 punct .,confirmed
R804 T1392 T1393 amod Homozygous,mutants
R805 T1393 T1394 nsubj mutants,showed
R806 T1395 T1396 det no,defects
R807 T1396 T1394 dobj defects,showed
R808 T1397 T1396 amod noticeable,defects
R809 T1398 T1394 prep at,showed
R810 T1399 T1398 pobj birth,at
R811 T1400 T1394 cc and,showed
R812 T1401 T1394 conj were,showed
R813 T1402 T1401 acomp indistinguishable,were
R814 T1403 T1402 prep from,indistinguishable
R815 T1404 T1405 amod wild,type
R816 T1405 T1407 nmod type,littermates
R817 T1406 T1405 punct -,type
R818 T1407 T1403 pobj littermates,from
R819 T1408 T1405 cc or,type
R820 T1409 T1405 conj heterozygous,type
R821 T1410 T1401 prep during,were
R822 T1411 T1412 det the,week
R823 T1412 T1410 pobj week,during
R824 T1413 T1412 amod first,week
R825 T1414 T1394 punct .,showed
R826 T1416 T1417 prep At,were
R827 T1418 T1419 amod postnatal,day
R828 T1419 T1416 pobj day,At
R829 T1420 T1419 nummod 10,day
R830 T1421 T1419 punct (,day
R831 T1422 T1419 appos P10,day
R832 T1423 T1417 punct ),were
R833 T1424 T1417 punct ", ",were
R834 T1425 T1417 nsubj most,were
R835 T1426 T1425 prep of,most
R836 T1427 T1428 det the,mutants
R837 T1428 T1426 pobj mutants,of
R838 T1429 T1428 amod homozygous,mutants
R839 T1430 T1417 acomp distinguishable,were
R840 T1431 T1430 prep by,distinguishable
R841 T1432 T1431 pobj abnormalities,by
R842 T1433 T1434 amod such,as
R843 T1434 T1432 prep as,abnormalities
R844 T1435 T1436 amod reduced,weight
R845 T1436 T1434 pobj weight,as
R846 T1437 T1436 compound body,weight
R847 T1438 T1436 cc and,weight
R848 T1439 T1440 amod uncoordinated,movements
R849 T1440 T1436 conj movements,weight
R850 T1441 T1440 prep of,movements
R851 T1442 T1443 poss their,limbs
R852 T1443 T1441 pobj limbs,of
R853 T1444 T1417 punct .,were
R854 T1446 T1447 prep After,displayed
R855 T1448 T1446 pobj P10,After
R856 T1449 T1447 punct ", ",displayed
R857 T1450 T1451 det all,homozygotes
R858 T1451 T1447 nsubj homozygotes,displayed
R859 T1452 T1453 amod severe,ataxia
R860 T1453 T1447 dobj ataxia,displayed
R861 T1454 T1455 punct (,Fig.
R862 T1455 T1447 parataxis Fig.,displayed
R863 T1456 T1455 nummod 2,Fig.
R864 T1457 T1455 punct ),Fig.
R865 T1458 T1447 cc and,displayed
R866 T1459 T1447 conj began,displayed
R867 T1460 T1461 aux to,die
R868 T1461 T1459 xcomp die,began
R869 T1462 T1447 punct .,displayed
R870 T1464 T1465 aux To,measure
R871 T1465 T1466 advcl measure,backcrossed
R872 T1467 T1468 det the,rate
R873 T1468 T1465 dobj rate,measure
R874 T1469 T1468 compound survival,rate
R875 T1470 T1468 cc and,rate
R876 T1471 T1472 compound body,weight
R877 T1472 T1468 conj weight,rate
R878 T1473 T1468 prep of,rate
R879 T1474 T1475 det each,genotype
R880 T1475 T1473 pobj genotype,of
R881 T1476 T1465 advmod precisely,measure
R882 T1477 T1466 punct ", ",backcrossed
R883 T1478 T1466 nsubj we,backcrossed
R884 T1479 T1480 amod heterozygous,mutants
R885 T1480 T1466 dobj mutants,backcrossed
R886 T1481 T1480 amod male,mutants
R887 T1482 T1466 prep to,backcrossed
R888 T1483 T1484 nmod C57BL,females
R889 T1484 T1482 pobj females,to
R890 T1485 T1483 punct /,C57BL
R891 T1486 T1483 nummod 6,C57BL
R892 T1487 T1488 amod more,6
R893 T1488 T1490 nummod 6,times
R894 T1489 T1488 quantmod than,6
R895 T1490 T1466 npadvmod times,backcrossed
R896 T1491 T1466 punct .,backcrossed
R897 T1493 T1494 det The,males
R898 T1494 T1501 nsubjpass males,intercrossed
R899 T1495 T1494 amod resulting,males
R900 T1496 T1497 punct (,N
R901 T1497 T1494 parataxis N,males
R902 T1498 T1497 nummod 6,N
R903 T1499 T1497 punct ),N
R904 T1500 T1494 amod heterozygous,males
R905 T1502 T1494 cc and,males
R906 T1503 T1494 conj females,males
R907 T1504 T1501 auxpass were,intercrossed
R908 T1505 T1501 cc and,intercrossed
R909 T1506 T1507 det the,offspring
R910 T1507 T1509 nsubjpass offspring,analysed
R911 T1508 T1507 amod produced,offspring
R912 T1509 T1501 conj analysed,intercrossed
R913 T1510 T1509 auxpass were,analysed
R914 T1511 T1509 punct .,analysed
R915 T1513 T1514 det The,numbers
R916 T1514 T1515 nsubjpass numbers,shown
R917 T1516 T1514 prep of,numbers
R918 T1517 T1516 pobj survivors,of
R919 T1518 T1517 prep of,survivors
R920 T1519 T1520 det each,genotype
R921 T1520 T1518 pobj genotype,of
R922 T1521 T1522 det every,days
R923 T1522 T1514 npadvmod days,numbers
R924 T1523 T1522 nummod 5,days
R925 T1524 T1515 auxpass are,shown
R926 T1525 T1515 prep in,shown
R927 T1526 T1525 pobj Table,in
R928 T1527 T1526 nummod 1,Table
R929 T1528 T1515 punct .,shown
R930 T1530 T1531 prep At,was
R931 T1532 T1530 pobj P10,At
R932 T1533 T1531 punct ", ",was
R933 T1534 T1535 det the,ratio
R934 T1535 T1531 nsubj ratio,was
R935 T1536 T1535 prep of,ratio
R936 T1537 T1538 det each,genotype
R937 T1538 T1536 pobj genotype,of
R938 T1539 T1531 prep in,was
R939 T1540 T1541 amod close,accordance
R940 T1541 T1539 pobj accordance,in
R941 T1542 T1541 prep with,accordance
R942 T1543 T1544 det the,ratio
R943 T1544 T1542 pobj ratio,with
R944 T1545 T1544 amod Mendelian,ratio
R945 T1546 T1547 punct (,%
R946 T1547 T1541 parataxis %,accordance
R947 T1548 T1547 nummod 20.5,%
R948 T1549 T1547 punct +,%
R949 T1550 T1547 punct /,%
R950 T1551 T1547 punct +,%
R951 T1552 T1547 punct ", ",%
R952 T1553 T1554 nummod 55.1,%
R953 T1554 T1547 appos %,%
R954 T1555 T1554 punct +,%
R955 T1556 T1554 punct /,%
R956 T1557 T1554 punct -,%
R957 T1558 T1547 punct ", ",%
R958 T1559 T1560 nummod 24.4,%
R959 T1560 T1547 appos %,%
R960 T1561 T1560 punct -,%
R961 T1562 T1560 punct /,%
R962 T1563 T1560 punct -,%
R963 T1564 T1547 punct ;,%
R964 T1565 T1566 nsubj n,78
R965 T1566 T1547 ccomp 78,%
R966 T1567 T1566 punct =,78
R967 T1568 T1547 punct ),%
R968 T1569 T1531 punct .,was
R969 T1571 T1572 det This,result
R970 T1572 T1573 nsubj result,shows
R971 T1574 T1575 mark that,is
R972 T1575 T1573 ccomp is,shows
R973 T1576 T1575 nsubj ADAM22,is
R974 T1577 T1575 neg not,is
R975 T1578 T1575 acomp essential,is
R976 T1579 T1578 prep for,essential
R977 T1580 T1579 pobj embryogenesis,for
R978 T1581 T1573 punct .,shows
R979 T1583 T1584 prep At,was
R980 T1585 T1583 pobj P10,At
R981 T1586 T1584 punct ", ",was
R982 T1587 T1588 det the,weight
R983 T1588 T1584 nsubj weight,was
R984 T1589 T1588 amod average,weight
R985 T1590 T1588 compound body,weight
R986 T1591 T1588 prep of,weight
R987 T1592 T1593 amod homozygous,mutants
R988 T1593 T1591 pobj mutants,of
R989 T1594 T1595 advmod approximately,half
R990 T1595 T1584 attr half,was
R991 T1596 T1595 det that,half
R992 T1597 T1595 prep of,half
R993 T1598 T1599 amod wild,type
R994 T1599 T1601 nmod type,littermates
R995 T1600 T1599 punct -,type
R996 T1601 T1597 pobj littermates,of
R997 T1602 T1599 cc and,type
R998 T1603 T1599 conj heterozygous,type
R999 T1604 T1605 punct (,Table