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PMC:1142324 / 17982-18941 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T5226 0-3 NN denotes His
T5228 3-4 HYPH denotes -
T5227 4-10 VBN denotes tagged
T5230 11-14 CC denotes and
T5231 15-18 NN denotes MBP
T5233 18-19 HYPH denotes -
T5232 19-24 VBN denotes fused
T5234 25-36 JJ denotes recombinant
T5229 37-44 NN denotes protein
T5236 45-55 VBG denotes containing
T5237 56-67 JJ denotes cytoplasmic
T5238 68-74 NN denotes domain
T5239 75-76 -LRB- denotes (
T5240 76-78 NN denotes cp
T5241 78-79 -RRB- denotes )
T5242 80-82 IN denotes of
T5243 83-88 JJ denotes human
T5245 89-95 NN denotes ADAM22
T5244 96-103 NN denotes isoform
T5246 104-105 CD denotes 1
T5247 106-107 -LRB- denotes (
T5249 107-115 NN denotes position
T5250 115-117 : denotes :
T5251 117-120 CD denotes 756
T5253 120-121 SYM denotes –
T5252 121-124 CD denotes 906
T5254 124-126 , denotes ,
T5255 126-129 CD denotes 151
T5256 130-135 NN denotes amino
T5248 136-141 NNS denotes acids
T5257 142-144 IN denotes in
T5258 145-151 NN denotes length
T5259 151-152 -RRB- denotes )
T5260 153-157 VBD denotes were
T5235 158-166 VBN denotes produced
T5261 167-169 IN denotes in
T5262 170-172 NNP denotes E.
T5263 173-177 NNP denotes coli
T5264 177-178 . denotes .
T5265 178-297 sentence denotes His-tagged ADAM22-cp protein was purified in denatured condition using Ni-NTA resin (Qiagen GmbH) and dialysed in PBS.
T5266 179-182 NN denotes His
T5268 182-183 HYPH denotes -
T5267 183-189 VBN denotes tagged
T5270 190-196 NN denotes ADAM22
T5272 196-197 HYPH denotes -
T5271 197-199 NN denotes cp
T5269 200-207 NN denotes protein
T5274 208-211 VBD denotes was
T5273 212-220 VBN denotes purified
T5275 221-223 IN denotes in
T5276 224-233 VBN denotes denatured
T5277 234-243 NN denotes condition
T5278 244-249 VBG denotes using
T5279 250-252 NN denotes Ni
T5281 252-253 HYPH denotes -
T5280 253-256 NN denotes NTA
T5282 257-262 NN denotes resin
T5283 263-264 -LRB- denotes (
T5285 264-270 NNP denotes Qiagen
T5284 271-275 NNP denotes GmbH
T5286 275-276 -RRB- denotes )
T5287 277-280 CC denotes and
T5288 281-289 VBN denotes dialysed
T5289 290-292 IN denotes in
T5290 293-296 NN denotes PBS
T5291 296-297 . denotes .
T5292 297-448 sentence denotes Precipitated protein was recovered and was mixed with Freund's complete adjuvant (Invitrogen), then, the mixture was used for immunization of rabbits.
T5293 298-310 VBN denotes Precipitated
T5294 311-318 NN denotes protein
T5296 319-322 VBD denotes was
T5295 323-332 VBN denotes recovered
T5298 333-336 CC denotes and
T5299 337-340 VBD denotes was
T5300 341-346 VBN denotes mixed
T5301 347-351 IN denotes with
T5302 352-358 NNP denotes Freund
T5304 358-360 POS denotes 's
T5305 361-369 JJ denotes complete
T5303 370-378 NN denotes adjuvant
T5306 379-380 -LRB- denotes (
T5307 380-390 NNP denotes Invitrogen
T5308 390-391 -RRB- denotes )
T5309 391-393 , denotes ,
T5310 393-397 RB denotes then
T5311 397-399 , denotes ,
T5312 399-402 DT denotes the
T5313 403-410 NN denotes mixture
T5314 411-414 VBD denotes was
T5297 415-419 VBN denotes used
T5315 420-423 IN denotes for
T5316 424-436 NN denotes immunization
T5317 437-439 IN denotes of
T5318 440-447 NNS denotes rabbits
T5319 447-448 . denotes .
T5320 448-597 sentence denotes The antiserum raised against the His-tagged ADAM22-cp protein was incubated with MBP-fused ADAM22-cp protein coupled to Affi-Gel 10 beads (Bio-Rad).
T5321 449-452 DT denotes The
T5322 453-462 NN denotes antiserum
T5324 463-469 VBN denotes raised
T5325 470-477 IN denotes against
T5326 478-481 DT denotes the
T5328 482-485 NN denotes His
T5330 485-486 HYPH denotes -
T5329 486-492 VBN denotes tagged
T5331 493-499 NN denotes ADAM22
T5333 499-500 HYPH denotes -
T5332 500-502 NN denotes cp
T5327 503-510 NN denotes protein
T5334 511-514 VBD denotes was
T5323 515-524 VBN denotes incubated
T5335 525-529 IN denotes with
T5336 530-533 NN denotes MBP
T5338 533-534 HYPH denotes -
T5337 534-539 VBN denotes fused
T5340 540-546 NN denotes ADAM22
T5342 546-547 HYPH denotes -
T5341 547-549 NN denotes cp
T5339 550-557 NN denotes protein
T5343 558-565 VBN denotes coupled
T5344 566-568 IN denotes to
T5345 569-573 NN denotes Affi
T5347 573-574 HYPH denotes -
T5346 574-577 NN denotes Gel
T5349 578-580 CD denotes 10
T5348 581-586 NNS denotes beads
T5350 587-588 -LRB- denotes (
T5352 588-591 NNP denotes Bio
T5353 591-592 HYPH denotes -
T5351 592-595 NNP denotes Rad
T5354 595-596 -RRB- denotes )
T5355 596-597 . denotes .
T5356 597-740 sentence denotes Beads with bound antibodies were washed in PBS, and the bound antibodies were eluted with 100 mM glycine, pH 3.0, and neutralized immediately.
T5357 598-603 NNS denotes Beads
T5359 604-608 IN denotes with
T5360 609-614 VBN denotes bound
T5361 615-625 NNS denotes antibodies
T5362 626-630 VBD denotes were
T5358 631-637 VBN denotes washed
T5363 638-640 IN denotes in
T5364 641-644 NN denotes PBS
T5365 644-646 , denotes ,
T5366 646-649 CC denotes and
T5367 650-653 DT denotes the
T5369 654-659 VBN denotes bound
T5368 660-670 NNS denotes antibodies
T5371 671-675 VBD denotes were
T5370 676-682 VBN denotes eluted
T5372 683-687 IN denotes with
T5373 688-691 CD denotes 100
T5374 692-694 NN denotes mM
T5375 695-702 NN denotes glycine
T5376 702-704 , denotes ,
T5377 704-706 NN denotes pH
T5378 707-710 CD denotes 3.0
T5379 710-712 , denotes ,
T5380 712-715 CC denotes and
T5381 716-727 VBN denotes neutralized
T5382 728-739 RB denotes immediately
T5383 739-740 . denotes .
T5384 740-855 sentence denotes Using the affinity purified antibody, both human and mouse ADAM22 proteins were detected by Western blot analysis.
T5385 741-746 VBG denotes Using
T5387 747-750 DT denotes the
T5389 751-759 NN denotes affinity
T5390 760-768 VBN denotes purified
T5388 769-777 NN denotes antibody
T5391 777-779 , denotes ,
T5392 779-783 CC denotes both
T5394 784-789 JJ denotes human
T5395 790-793 CC denotes and
T5393 794-799 NN denotes mouse
T5397 800-806 NN denotes ADAM22
T5396 807-815 NN denotes proteins
T5398 816-820 VBD denotes were
T5386 821-829 VBN denotes detected
T5399 830-832 IN denotes by
T5400 833-840 NNP denotes Western
T5401 841-845 NN denotes blot
T5402 846-854 NN denotes analysis
T5403 854-855 . denotes .
T5404 855-959 sentence denotes However, unfortunately the antibody was not suitable for immunohistochemical analysis of mouse tissues.
T5405 856-863 RB denotes However
T5407 863-865 , denotes ,
T5408 865-878 RB denotes unfortunately
T5409 879-882 DT denotes the
T5410 883-891 NN denotes antibody
T5406 892-895 VBD denotes was
T5411 896-899 RB denotes not
T5412 900-908 JJ denotes suitable
T5413 909-912 IN denotes for
T5414 913-932 JJ denotes immunohistochemical
T5415 933-941 NN denotes analysis
T5416 942-944 IN denotes of
T5417 945-950 NN denotes mouse
T5418 951-958 NNS denotes tissues
T5419 958-959 . denotes .
R3230 T5226 T5227 npadvmod His,tagged
R3231 T5227 T5229 amod tagged,protein
R3232 T5228 T5227 punct -,tagged
R3233 T5229 T5235 nsubjpass protein,produced
R3234 T5230 T5227 cc and,tagged
R3235 T5231 T5232 npadvmod MBP,fused
R3236 T5232 T5227 conj fused,tagged
R3237 T5233 T5232 punct -,fused
R3238 T5234 T5229 amod recombinant,protein
R3239 T5236 T5229 acl containing,protein
R3240 T5237 T5238 amod cytoplasmic,domain
R3241 T5238 T5236 dobj domain,containing
R3242 T5239 T5238 punct (,domain
R3243 T5240 T5238 appos cp,domain
R3244 T5241 T5238 punct ),domain
R3245 T5242 T5238 prep of,domain
R3246 T5243 T5244 amod human,isoform
R3247 T5244 T5242 pobj isoform,of
R3248 T5245 T5244 compound ADAM22,isoform
R3249 T5246 T5244 nummod 1,isoform
R3250 T5247 T5248 punct (,acids
R3251 T5248 T5244 parataxis acids,isoform
R3252 T5249 T5248 dep position,acids
R3253 T5250 T5249 punct : ,position
R3254 T5251 T5252 nummod 756,906
R3255 T5252 T5249 appos 906,position
R3256 T5253 T5252 punct –,906
R3257 T5254 T5248 punct ", ",acids
R3258 T5255 T5248 nummod 151,acids
R3259 T5256 T5248 compound amino,acids
R3260 T5257 T5248 prep in,acids
R3261 T5258 T5257 pobj length,in
R3262 T5259 T5248 punct ),acids
R3263 T5260 T5235 auxpass were,produced
R3264 T5261 T5235 prep in,produced
R3265 T5262 T5263 compound E.,coli
R3266 T5263 T5261 pobj coli,in
R3267 T5264 T5235 punct .,produced
R3268 T5266 T5267 npadvmod His,tagged
R3269 T5267 T5269 amod tagged,protein
R3270 T5268 T5267 punct -,tagged
R3271 T5269 T5273 nsubjpass protein,purified
R3272 T5270 T5271 compound ADAM22,cp
R3273 T5271 T5269 compound cp,protein
R3274 T5272 T5271 punct -,cp
R3275 T5274 T5273 auxpass was,purified
R3276 T5275 T5273 prep in,purified
R3277 T5276 T5277 amod denatured,condition
R3278 T5277 T5275 pobj condition,in
R3279 T5278 T5273 advcl using,purified
R3280 T5279 T5280 compound Ni,NTA
R3281 T5280 T5282 compound NTA,resin
R3282 T5281 T5280 punct -,NTA
R3283 T5282 T5278 dobj resin,using
R3284 T5283 T5284 punct (,GmbH
R3285 T5284 T5282 parataxis GmbH,resin
R3286 T5285 T5284 compound Qiagen,GmbH
R3287 T5286 T5284 punct ),GmbH
R3288 T5287 T5273 cc and,purified
R3289 T5288 T5273 conj dialysed,purified
R3290 T5289 T5288 prep in,dialysed
R3291 T5290 T5289 pobj PBS,in
R3292 T5291 T5273 punct .,purified
R3293 T5293 T5294 amod Precipitated,protein
R3294 T5294 T5295 nsubjpass protein,recovered
R3295 T5295 T5297 ccomp recovered,used
R3296 T5296 T5295 auxpass was,recovered
R3297 T5298 T5295 cc and,recovered
R3298 T5299 T5300 auxpass was,mixed
R3299 T5300 T5295 conj mixed,recovered
R3300 T5301 T5300 prep with,mixed
R3301 T5302 T5303 poss Freund,adjuvant
R3302 T5303 T5301 pobj adjuvant,with
R3303 T5304 T5302 case 's,Freund
R3304 T5305 T5303 amod complete,adjuvant
R3305 T5306 T5307 punct (,Invitrogen
R3306 T5307 T5303 parataxis Invitrogen,adjuvant
R3307 T5308 T5307 punct ),Invitrogen
R3308 T5309 T5297 punct ", ",used
R3309 T5310 T5297 advmod then,used
R3310 T5311 T5297 punct ", ",used
R3311 T5312 T5313 det the,mixture
R3312 T5313 T5297 nsubjpass mixture,used
R3313 T5314 T5297 auxpass was,used
R3314 T5315 T5297 prep for,used
R3315 T5316 T5315 pobj immunization,for
R3316 T5317 T5316 prep of,immunization
R3317 T5318 T5317 pobj rabbits,of
R3318 T5319 T5297 punct .,used
R3319 T5321 T5322 det The,antiserum
R3320 T5322 T5323 nsubjpass antiserum,incubated
R3321 T5324 T5322 acl raised,antiserum
R3322 T5325 T5324 prep against,raised
R3323 T5326 T5327 det the,protein
R3324 T5327 T5325 pobj protein,against
R3325 T5328 T5329 npadvmod His,tagged
R3326 T5329 T5327 amod tagged,protein
R3327 T5330 T5329 punct -,tagged
R3328 T5331 T5332 compound ADAM22,cp
R3329 T5332 T5327 compound cp,protein
R3330 T5333 T5332 punct -,cp
R3331 T5334 T5323 auxpass was,incubated
R3332 T5335 T5323 prep with,incubated
R3333 T5336 T5337 npadvmod MBP,fused
R3334 T5337 T5339 amod fused,protein
R3335 T5338 T5337 punct -,fused
R3336 T5339 T5335 pobj protein,with
R3337 T5340 T5341 compound ADAM22,cp
R3338 T5341 T5339 compound cp,protein
R3339 T5342 T5341 punct -,cp
R3340 T5343 T5339 acl coupled,protein
R3341 T5344 T5343 prep to,coupled
R3342 T5345 T5346 nmod Affi,Gel
R3343 T5346 T5348 nmod Gel,beads
R3344 T5347 T5346 punct -,Gel
R3345 T5348 T5344 pobj beads,to
R3346 T5349 T5346 nummod 10,Gel
R3347 T5350 T5351 punct (,Rad
R3348 T5351 T5348 parataxis Rad,beads
R3349 T5352 T5351 compound Bio,Rad
R3350 T5353 T5351 punct -,Rad
R3351 T5354 T5351 punct ),Rad
R3352 T5355 T5323 punct .,incubated
R3353 T5357 T5358 nsubjpass Beads,washed
R3354 T5359 T5357 prep with,Beads
R3355 T5360 T5361 amod bound,antibodies
R3356 T5361 T5359 pobj antibodies,with
R3357 T5362 T5358 auxpass were,washed
R3358 T5363 T5358 prep in,washed
R3359 T5364 T5363 pobj PBS,in
R3360 T5365 T5358 punct ", ",washed
R3361 T5366 T5358 cc and,washed
R3362 T5367 T5368 det the,antibodies
R3363 T5368 T5370 nsubjpass antibodies,eluted
R3364 T5369 T5368 amod bound,antibodies
R3365 T5370 T5358 conj eluted,washed
R3366 T5371 T5370 auxpass were,eluted
R3367 T5372 T5370 prep with,eluted
R3368 T5373 T5374 nummod 100,mM
R3369 T5374 T5375 compound mM,glycine
R3370 T5375 T5372 pobj glycine,with
R3371 T5376 T5375 punct ", ",glycine
R3372 T5377 T5375 npadvmod pH,glycine
R3373 T5378 T5377 nummod 3.0,pH
R3374 T5379 T5370 punct ", ",eluted
R3375 T5380 T5370 cc and,eluted
R3376 T5381 T5370 conj neutralized,eluted
R3377 T5382 T5381 advmod immediately,neutralized
R3378 T5383 T5370 punct .,eluted
R3379 T5385 T5386 advcl Using,detected
R3380 T5387 T5388 det the,antibody
R3381 T5388 T5385 dobj antibody,Using
R3382 T5389 T5390 npadvmod affinity,purified
R3383 T5390 T5388 amod purified,antibody
R3384 T5391 T5386 punct ", ",detected
R3385 T5392 T5393 preconj both,mouse
R3386 T5393 T5396 compound mouse,proteins
R3387 T5394 T5393 amod human,mouse
R3388 T5395 T5393 cc and,mouse
R3389 T5396 T5386 nsubjpass proteins,detected
R3390 T5397 T5396 compound ADAM22,proteins
R3391 T5398 T5386 auxpass were,detected
R3392 T5399 T5386 prep by,detected
R3393 T5400 T5401 compound Western,blot
R3394 T5401 T5402 compound blot,analysis
R3395 T5402 T5399 pobj analysis,by
R3396 T5403 T5386 punct .,detected
R3397 T5405 T5406 advmod However,was
R3398 T5407 T5406 punct ", ",was
R3399 T5408 T5406 advmod unfortunately,was
R3400 T5409 T5410 det the,antibody
R3401 T5410 T5406 nsubj antibody,was
R3402 T5411 T5406 neg not,was
R3403 T5412 T5406 acomp suitable,was
R3404 T5413 T5412 prep for,suitable
R3405 T5414 T5415 amod immunohistochemical,analysis
R3406 T5415 T5413 pobj analysis,for
R3407 T5416 T5415 prep of,analysis
R3408 T5417 T5418 compound mouse,tissues
R3409 T5418 T5416 pobj tissues,of
R3410 T5419 T5406 punct .,was