| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T2289 |
0-7 |
NN |
denotes |
Annexin |
| T2290 |
8-10 |
NN |
denotes |
A7 |
| T2292 |
11-13 |
VBZ |
denotes |
is |
| T2291 |
14-23 |
VBN |
denotes |
expressed |
| T2293 |
24-26 |
IN |
denotes |
in |
| T2294 |
27-30 |
DT |
denotes |
the |
| T2296 |
31-36 |
JJ |
denotes |
early |
| T2297 |
37-42 |
NN |
denotes |
mouse |
| T2295 |
43-49 |
NN |
denotes |
embryo |
| T2298 |
49-330 |
sentence |
denotes |
First we examined the expression of Annexin A7 in ES cells (Bruce4, established from C57BL/6J mice) and the early stages of mouse embryonic development at the mRNA level by northern blot analysis and at the protein level by Western blotting and immunohistochemistry, respectively. |
| T2299 |
50-55 |
RB |
denotes |
First |
| T2301 |
56-58 |
PRP |
denotes |
we |
| T2300 |
59-67 |
VBD |
denotes |
examined |
| T2302 |
68-71 |
DT |
denotes |
the |
| T2303 |
72-82 |
NN |
denotes |
expression |
| T2304 |
83-85 |
IN |
denotes |
of |
| T2305 |
86-93 |
NN |
denotes |
Annexin |
| T2306 |
94-96 |
NN |
denotes |
A7 |
| T2307 |
97-99 |
IN |
denotes |
in |
| T2308 |
100-102 |
NN |
denotes |
ES |
| T2309 |
103-108 |
NNS |
denotes |
cells |
| T2310 |
109-110 |
-LRB- |
denotes |
( |
| T2311 |
110-116 |
NN |
denotes |
Bruce4 |
| T2312 |
116-118 |
, |
denotes |
, |
| T2313 |
118-129 |
VBN |
denotes |
established |
| T2314 |
130-134 |
IN |
denotes |
from |
| T2315 |
135-140 |
NN |
denotes |
C57BL |
| T2317 |
140-141 |
HYPH |
denotes |
/ |
| T2316 |
141-143 |
NN |
denotes |
6J |
| T2318 |
144-148 |
NNS |
denotes |
mice |
| T2319 |
148-149 |
-RRB- |
denotes |
) |
| T2320 |
150-153 |
CC |
denotes |
and |
| T2321 |
154-157 |
DT |
denotes |
the |
| T2323 |
158-163 |
JJ |
denotes |
early |
| T2322 |
164-170 |
NNS |
denotes |
stages |
| T2324 |
171-173 |
IN |
denotes |
of |
| T2325 |
174-179 |
NN |
denotes |
mouse |
| T2327 |
180-189 |
JJ |
denotes |
embryonic |
| T2326 |
190-201 |
NN |
denotes |
development |
| T2328 |
202-204 |
IN |
denotes |
at |
| T2329 |
205-208 |
DT |
denotes |
the |
| T2331 |
209-213 |
NN |
denotes |
mRNA |
| T2330 |
214-219 |
NN |
denotes |
level |
| T2332 |
220-222 |
IN |
denotes |
by |
| T2333 |
223-231 |
NNP |
denotes |
northern |
| T2334 |
232-236 |
NN |
denotes |
blot |
| T2335 |
237-245 |
NN |
denotes |
analysis |
| T2336 |
246-249 |
CC |
denotes |
and |
| T2337 |
250-252 |
IN |
denotes |
at |
| T2338 |
253-256 |
DT |
denotes |
the |
| T2340 |
257-264 |
NN |
denotes |
protein |
| T2339 |
265-270 |
NN |
denotes |
level |
| T2341 |
271-273 |
IN |
denotes |
by |
| T2342 |
274-281 |
NNP |
denotes |
Western |
| T2343 |
282-290 |
NN |
denotes |
blotting |
| T2344 |
291-294 |
CC |
denotes |
and |
| T2345 |
295-315 |
NN |
denotes |
immunohistochemistry |
| T2346 |
315-317 |
, |
denotes |
, |
| T2347 |
317-329 |
RB |
denotes |
respectively |
| T2348 |
329-330 |
. |
denotes |
. |
| T2349 |
330-535 |
sentence |
denotes |
Northern blot analysis shows in ES-cells and at embryonic days E7, E11, E15, and E17 two mRNA species of 1.8 kb and 2.4 kb, which result from alternative splicing in the untranslated 3'end (Fig. 1A) [11]. |
| T2350 |
331-339 |
NNP |
denotes |
Northern |
| T2351 |
340-344 |
NN |
denotes |
blot |
| T2352 |
345-353 |
NN |
denotes |
analysis |
| T2353 |
354-359 |
VBZ |
denotes |
shows |
| T2354 |
360-362 |
IN |
denotes |
in |
| T2355 |
363-365 |
NN |
denotes |
ES |
| T2357 |
365-366 |
HYPH |
denotes |
- |
| T2356 |
366-371 |
NNS |
denotes |
cells |
| T2358 |
372-375 |
CC |
denotes |
and |
| T2359 |
376-378 |
IN |
denotes |
at |
| T2360 |
379-388 |
JJ |
denotes |
embryonic |
| T2362 |
389-393 |
NNS |
denotes |
days |
| T2361 |
394-396 |
NN |
denotes |
E7 |
| T2363 |
396-398 |
, |
denotes |
, |
| T2364 |
398-401 |
NN |
denotes |
E11 |
| T2365 |
401-403 |
, |
denotes |
, |
| T2366 |
403-406 |
NN |
denotes |
E15 |
| T2367 |
406-408 |
, |
denotes |
, |
| T2368 |
408-411 |
CC |
denotes |
and |
| T2369 |
412-415 |
NN |
denotes |
E17 |
| T2370 |
416-419 |
CD |
denotes |
two |
| T2372 |
420-424 |
NN |
denotes |
mRNA |
| T2371 |
425-432 |
NNS |
denotes |
species |
| T2373 |
433-435 |
IN |
denotes |
of |
| T2374 |
436-439 |
CD |
denotes |
1.8 |
| T2375 |
440-442 |
NN |
denotes |
kb |
| T2376 |
443-446 |
CC |
denotes |
and |
| T2377 |
447-450 |
CD |
denotes |
2.4 |
| T2378 |
451-453 |
NN |
denotes |
kb |
| T2379 |
453-455 |
, |
denotes |
, |
| T2380 |
455-460 |
WDT |
denotes |
which |
| T2381 |
461-467 |
VBP |
denotes |
result |
| T2382 |
468-472 |
IN |
denotes |
from |
| T2383 |
473-484 |
JJ |
denotes |
alternative |
| T2384 |
485-493 |
NN |
denotes |
splicing |
| T2385 |
494-496 |
IN |
denotes |
in |
| T2386 |
497-500 |
DT |
denotes |
the |
| T2388 |
501-513 |
JJ |
denotes |
untranslated |
| T2389 |
514-515 |
CD |
denotes |
3 |
| T2390 |
515-516 |
SYM |
denotes |
' |
| T2387 |
516-519 |
NN |
denotes |
end |
| T2391 |
520-521 |
-LRB- |
denotes |
( |
| T2392 |
521-525 |
NN |
denotes |
Fig. |
| T2393 |
526-528 |
CD |
denotes |
1A |
| T2394 |
528-529 |
-RRB- |
denotes |
) |
| T2395 |
530-531 |
-LRB- |
denotes |
[ |
| T2396 |
531-533 |
CD |
denotes |
11 |
| T2397 |
533-534 |
-RRB- |
denotes |
] |
| T2398 |
534-535 |
. |
denotes |
. |
| T2399 |
535-605 |
sentence |
denotes |
We found similar Annexin A7 mRNA levels in the four embryonic stages. |
| T2400 |
536-538 |
PRP |
denotes |
We |
| T2401 |
539-544 |
VBD |
denotes |
found |
| T2402 |
545-552 |
JJ |
denotes |
similar |
| T2404 |
553-560 |
NN |
denotes |
Annexin |
| T2405 |
561-563 |
NN |
denotes |
A7 |
| T2406 |
564-568 |
NN |
denotes |
mRNA |
| T2403 |
569-575 |
NNS |
denotes |
levels |
| T2407 |
576-578 |
IN |
denotes |
in |
| T2408 |
579-582 |
DT |
denotes |
the |
| T2410 |
583-587 |
CD |
denotes |
four |
| T2411 |
588-597 |
JJ |
denotes |
embryonic |
| T2409 |
598-604 |
NNS |
denotes |
stages |
| T2412 |
604-605 |
. |
denotes |
. |
| T2413 |
605-780 |
sentence |
denotes |
Reprobing with a β-actin probe verified equal loading; the appearance of a faster migrating mRNA species in addition to the 2.0 kb species is characteristic for β-actin [12]. |
| T2414 |
606-615 |
VBG |
denotes |
Reprobing |
| T2416 |
616-620 |
IN |
denotes |
with |
| T2417 |
621-622 |
DT |
denotes |
a |
| T2419 |
623-624 |
NN |
denotes |
β |
| T2421 |
624-625 |
HYPH |
denotes |
- |
| T2420 |
625-630 |
NN |
denotes |
actin |
| T2418 |
631-636 |
NN |
denotes |
probe |
| T2415 |
637-645 |
VBD |
denotes |
verified |
| T2423 |
646-651 |
JJ |
denotes |
equal |
| T2424 |
652-659 |
NN |
denotes |
loading |
| T2425 |
659-660 |
: |
denotes |
; |
| T2426 |
661-664 |
DT |
denotes |
the |
| T2427 |
665-675 |
NN |
denotes |
appearance |
| T2428 |
676-678 |
IN |
denotes |
of |
| T2429 |
679-680 |
DT |
denotes |
a |
| T2431 |
681-687 |
JJR |
denotes |
faster |
| T2432 |
688-697 |
VBG |
denotes |
migrating |
| T2433 |
698-702 |
NN |
denotes |
mRNA |
| T2430 |
703-710 |
NNS |
denotes |
species |
| T2434 |
711-713 |
IN |
denotes |
in |
| T2435 |
714-722 |
NN |
denotes |
addition |
| T2436 |
723-725 |
IN |
denotes |
to |
| T2437 |
726-729 |
DT |
denotes |
the |
| T2439 |
730-733 |
CD |
denotes |
2.0 |
| T2440 |
734-736 |
NN |
denotes |
kb |
| T2438 |
737-744 |
NNS |
denotes |
species |
| T2422 |
745-747 |
VBZ |
denotes |
is |
| T2441 |
748-762 |
JJ |
denotes |
characteristic |
| T2442 |
763-766 |
IN |
denotes |
for |
| T2443 |
767-768 |
NN |
denotes |
β |
| T2445 |
768-769 |
HYPH |
denotes |
- |
| T2444 |
769-774 |
NN |
denotes |
actin |
| T2446 |
775-776 |
-LRB- |
denotes |
[ |
| T2447 |
776-778 |
CD |
denotes |
12 |
| T2448 |
778-779 |
-RRB- |
denotes |
] |
| T2449 |
779-780 |
. |
denotes |
. |
| T2450 |
780-934 |
sentence |
denotes |
On the protein level, ES cells express only the smaller Annexin A7 isoform of 47 kDa (Fig. 1B, neuro-2a cells and heart tissue are included for control). |
| T2451 |
781-783 |
IN |
denotes |
On |
| T2453 |
784-787 |
DT |
denotes |
the |
| T2455 |
788-795 |
NN |
denotes |
protein |
| T2454 |
796-801 |
NN |
denotes |
level |
| T2456 |
801-803 |
, |
denotes |
, |
| T2457 |
803-805 |
NN |
denotes |
ES |
| T2458 |
806-811 |
NNS |
denotes |
cells |
| T2452 |
812-819 |
VBP |
denotes |
express |
| T2459 |
820-824 |
RB |
denotes |
only |
| T2461 |
825-828 |
DT |
denotes |
the |
| T2462 |
829-836 |
JJR |
denotes |
smaller |
| T2463 |
837-844 |
NN |
denotes |
Annexin |
| T2464 |
845-847 |
NN |
denotes |
A7 |
| T2460 |
848-855 |
NN |
denotes |
isoform |
| T2465 |
856-858 |
IN |
denotes |
of |
| T2466 |
859-861 |
CD |
denotes |
47 |
| T2467 |
862-865 |
NN |
denotes |
kDa |
| T2468 |
866-867 |
-LRB- |
denotes |
( |
| T2470 |
867-871 |
NN |
denotes |
Fig. |
| T2469 |
872-874 |
NN |
denotes |
1B |
| T2471 |
874-876 |
, |
denotes |
, |
| T2472 |
876-881 |
NN |
denotes |
neuro |
| T2474 |
881-882 |
HYPH |
denotes |
- |
| T2475 |
882-884 |
CD |
denotes |
2a |
| T2473 |
885-890 |
NNS |
denotes |
cells |
| T2477 |
891-894 |
CC |
denotes |
and |
| T2478 |
895-900 |
NN |
denotes |
heart |
| T2479 |
901-907 |
NN |
denotes |
tissue |
| T2480 |
908-911 |
VBP |
denotes |
are |
| T2476 |
912-920 |
VBN |
denotes |
included |
| T2481 |
921-924 |
IN |
denotes |
for |
| T2482 |
925-932 |
NN |
denotes |
control |
| T2483 |
932-933 |
-RRB- |
denotes |
) |
| T2484 |
933-934 |
. |
denotes |
. |
| T2485 |
934-1858 |
sentence |
denotes |
Figure 1 Expression of Annexin A7. (A) Northern blot analysis of RNA from ES-cells and embryos E7, E11, E15, E17 probed with a full length annexin A7 cDNA. Transcripts of 1.8 and 2.4 kb are detected in all stages examined (upper panel). Reprobing with a β-actin probe verified equal loading (lower panel, 2.0 kb band). (B) Murine ES cells (ES) express only the small Annexin A7 isoform. Both isoforms are expressed in neuroblastoma neuro-2a cells (N2A), heart (HRT) and brain tissue (CNS) from adult mice. Proteins from neuro-2a and ES cells, heart and brain were resolved by SDS polyacrylamide gel electrophoresis (12% acrylamide) and transferred to nitrocellulose membranes. The Western blot was probed with mAb 203–217 followed by incubation with a peroxidase coupled secondary antibody. Detection was with enhanced chemiluminescence. Immunohistochemistry confirmed the presence of the protein during early development. |
| T11654 |
945-955 |
NN |
denotes |
Expression |
| T11655 |
956-958 |
IN |
denotes |
of |
| T11656 |
959-966 |
NN |
denotes |
Annexin |
| T11657 |
967-969 |
NN |
denotes |
A7 |
| T11658 |
969-970 |
. |
denotes |
. |
| T11659 |
970-1091 |
sentence |
denotes |
(A) Northern blot analysis of RNA from ES-cells and embryos E7, E11, E15, E17 probed with a full length annexin A7 cDNA. |
| T11660 |
971-972 |
-LRB- |
denotes |
( |
| T11661 |
972-973 |
LS |
denotes |
A |
| T11663 |
973-974 |
-RRB- |
denotes |
) |
| T11664 |
975-983 |
NNP |
denotes |
Northern |
| T11665 |
984-988 |
NN |
denotes |
blot |
| T11662 |
989-997 |
NN |
denotes |
analysis |
| T11666 |
998-1000 |
IN |
denotes |
of |
| T11667 |
1001-1004 |
NN |
denotes |
RNA |
| T11668 |
1005-1009 |
IN |
denotes |
from |
| T11669 |
1010-1012 |
NN |
denotes |
ES |
| T11671 |
1012-1013 |
HYPH |
denotes |
- |
| T11670 |
1013-1018 |
NNS |
denotes |
cells |
| T11672 |
1019-1022 |
CC |
denotes |
and |
| T11673 |
1023-1030 |
NNS |
denotes |
embryos |
| T11675 |
1031-1033 |
NN |
denotes |
E7 |
| T11676 |
1033-1035 |
, |
denotes |
, |
| T11677 |
1035-1038 |
NN |
denotes |
E11 |
| T11678 |
1038-1040 |
, |
denotes |
, |
| T11679 |
1040-1043 |
NN |
denotes |
E15 |
| T11680 |
1043-1045 |
, |
denotes |
, |
| T11674 |
1045-1048 |
NN |
denotes |
E17 |
| T11681 |
1049-1055 |
VBD |
denotes |
probed |
| T11682 |
1056-1060 |
IN |
denotes |
with |
| T11683 |
1061-1062 |
DT |
denotes |
a |
| T11685 |
1063-1067 |
JJ |
denotes |
full |
| T11686 |
1068-1074 |
NN |
denotes |
length |
| T11687 |
1075-1082 |
NN |
denotes |
annexin |
| T11688 |
1083-1085 |
NN |
denotes |
A7 |
| T11684 |
1086-1090 |
NN |
denotes |
cDNA |
| T11689 |
1090-1091 |
. |
denotes |
. |
| T11690 |
1091-1172 |
sentence |
denotes |
Transcripts of 1.8 and 2.4 kb are detected in all stages examined (upper panel). |
| T11691 |
1092-1103 |
NNS |
denotes |
Transcripts |
| T11693 |
1104-1106 |
IN |
denotes |
of |
| T11694 |
1107-1110 |
CD |
denotes |
1.8 |
| T11696 |
1111-1114 |
CC |
denotes |
and |
| T11697 |
1115-1118 |
CD |
denotes |
2.4 |
| T11695 |
1119-1121 |
NN |
denotes |
kb |
| T11698 |
1122-1125 |
VBP |
denotes |
are |
| T11692 |
1126-1134 |
VBN |
denotes |
detected |
| T11699 |
1135-1137 |
IN |
denotes |
in |
| T11700 |
1138-1141 |
DT |
denotes |
all |
| T11701 |
1142-1148 |
NNS |
denotes |
stages |
| T11702 |
1149-1157 |
VBN |
denotes |
examined |
| T11703 |
1158-1159 |
-LRB- |
denotes |
( |
| T11705 |
1159-1164 |
JJ |
denotes |
upper |
| T11704 |
1165-1170 |
NN |
denotes |
panel |
| T11706 |
1170-1171 |
-RRB- |
denotes |
) |
| T11707 |
1171-1172 |
. |
denotes |
. |
| T11708 |
1172-1254 |
sentence |
denotes |
Reprobing with a β-actin probe verified equal loading (lower panel, 2.0 kb band). |
| T11709 |
1173-1182 |
VBG |
denotes |
Reprobing |
| T11711 |
1183-1187 |
IN |
denotes |
with |
| T11712 |
1188-1189 |
DT |
denotes |
a |
| T11714 |
1190-1191 |
NN |
denotes |
β |
| T11716 |
1191-1192 |
HYPH |
denotes |
- |
| T11715 |
1192-1197 |
NN |
denotes |
actin |
| T11713 |
1198-1203 |
NN |
denotes |
probe |
| T11710 |
1204-1212 |
VBD |
denotes |
verified |
| T11717 |
1213-1218 |
JJ |
denotes |
equal |
| T11718 |
1219-1226 |
NN |
denotes |
loading |
| T11719 |
1227-1228 |
-LRB- |
denotes |
( |
| T11721 |
1228-1233 |
JJ |
denotes |
lower |
| T11722 |
1234-1239 |
NN |
denotes |
panel |
| T11723 |
1239-1241 |
, |
denotes |
, |
| T11724 |
1241-1244 |
CD |
denotes |
2.0 |
| T11725 |
1245-1247 |
NN |
denotes |
kb |
| T11720 |
1248-1252 |
NN |
denotes |
band |
| T11726 |
1252-1253 |
-RRB- |
denotes |
) |
| T11727 |
1253-1254 |
. |
denotes |
. |
| T11728 |
1254-1322 |
sentence |
denotes |
(B) Murine ES cells (ES) express only the small Annexin A7 isoform. |
| T11729 |
1255-1256 |
-LRB- |
denotes |
( |
| T11730 |
1256-1257 |
LS |
denotes |
B |
| T11732 |
1257-1258 |
-RRB- |
denotes |
) |
| T11733 |
1259-1265 |
JJ |
denotes |
Murine |
| T11735 |
1266-1268 |
NN |
denotes |
ES |
| T11734 |
1269-1274 |
NNS |
denotes |
cells |
| T11736 |
1275-1276 |
-LRB- |
denotes |
( |
| T11737 |
1276-1278 |
NN |
denotes |
ES |
| T11738 |
1278-1279 |
-RRB- |
denotes |
) |
| T11731 |
1280-1287 |
VBP |
denotes |
express |
| T11739 |
1288-1292 |
RB |
denotes |
only |
| T11741 |
1293-1296 |
DT |
denotes |
the |
| T11742 |
1297-1302 |
JJ |
denotes |
small |
| T11743 |
1303-1310 |
NN |
denotes |
Annexin |
| T11744 |
1311-1313 |
NN |
denotes |
A7 |
| T11740 |
1314-1321 |
NN |
denotes |
isoform |
| T11745 |
1321-1322 |
. |
denotes |
. |
| T11746 |
1322-1441 |
sentence |
denotes |
Both isoforms are expressed in neuroblastoma neuro-2a cells (N2A), heart (HRT) and brain tissue (CNS) from adult mice. |
| T11747 |
1323-1327 |
DT |
denotes |
Both |
| T11748 |
1328-1336 |
NNS |
denotes |
isoforms |
| T11750 |
1337-1340 |
VBP |
denotes |
are |
| T11749 |
1341-1350 |
VBN |
denotes |
expressed |
| T11751 |
1351-1353 |
IN |
denotes |
in |
| T11752 |
1354-1367 |
NN |
denotes |
neuroblastoma |
| T11754 |
1368-1373 |
NN |
denotes |
neuro |
| T11756 |
1373-1374 |
HYPH |
denotes |
- |
| T11755 |
1374-1376 |
NN |
denotes |
2a |
| T11753 |
1377-1382 |
NNS |
denotes |
cells |
| T11757 |
1383-1384 |
-LRB- |
denotes |
( |
| T11758 |
1384-1387 |
NN |
denotes |
N2A |
| T11759 |
1387-1388 |
-RRB- |
denotes |
) |
| T11760 |
1388-1390 |
, |
denotes |
, |
| T11761 |
1390-1395 |
NN |
denotes |
heart |
| T11762 |
1396-1397 |
-LRB- |
denotes |
( |
| T11763 |
1397-1400 |
NN |
denotes |
HRT |
| T11764 |
1400-1401 |
-RRB- |
denotes |
) |
| T11765 |
1402-1405 |
CC |
denotes |
and |
| T11766 |
1406-1411 |
NN |
denotes |
brain |
| T11767 |
1412-1418 |
NN |
denotes |
tissue |
| T11768 |
1419-1420 |
-LRB- |
denotes |
( |
| T11769 |
1420-1423 |
NN |
denotes |
CNS |
| T11770 |
1423-1424 |
-RRB- |
denotes |
) |
| T11771 |
1425-1429 |
IN |
denotes |
from |
| T11772 |
1430-1435 |
JJ |
denotes |
adult |
| T11773 |
1436-1440 |
NNS |
denotes |
mice |
| T11774 |
1440-1441 |
. |
denotes |
. |
| T11775 |
1441-1612 |
sentence |
denotes |
Proteins from neuro-2a and ES cells, heart and brain were resolved by SDS polyacrylamide gel electrophoresis (12% acrylamide) and transferred to nitrocellulose membranes. |
| T11776 |
1442-1450 |
NNS |
denotes |
Proteins |
| T11778 |
1451-1455 |
IN |
denotes |
from |
| T11779 |
1456-1461 |
NN |
denotes |
neuro |
| T11781 |
1461-1462 |
HYPH |
denotes |
- |
| T11780 |
1462-1464 |
NN |
denotes |
2a |
| T11782 |
1465-1468 |
CC |
denotes |
and |
| T11783 |
1469-1471 |
NN |
denotes |
ES |
| T11784 |
1472-1477 |
NNS |
denotes |
cells |
| T11785 |
1477-1479 |
, |
denotes |
, |
| T11786 |
1479-1484 |
NN |
denotes |
heart |
| T11787 |
1485-1488 |
CC |
denotes |
and |
| T11788 |
1489-1494 |
NN |
denotes |
brain |
| T11789 |
1495-1499 |
VBD |
denotes |
were |
| T11777 |
1500-1508 |
VBN |
denotes |
resolved |
| T11790 |
1509-1511 |
IN |
denotes |
by |
| T11791 |
1512-1515 |
NN |
denotes |
SDS |
| T11793 |
1516-1530 |
NN |
denotes |
polyacrylamide |
| T11794 |
1531-1534 |
NN |
denotes |
gel |
| T11792 |
1535-1550 |
NN |
denotes |
electrophoresis |
| T11795 |
1551-1552 |
-LRB- |
denotes |
( |
| T11797 |
1552-1554 |
CD |
denotes |
12 |
| T11798 |
1554-1555 |
NN |
denotes |
% |
| T11796 |
1556-1566 |
NN |
denotes |
acrylamide |
| T11799 |
1566-1567 |
-RRB- |
denotes |
) |
| T11800 |
1568-1571 |
CC |
denotes |
and |
| T11801 |
1572-1583 |
VBN |
denotes |
transferred |
| T11802 |
1584-1586 |
IN |
denotes |
to |
| T11803 |
1587-1601 |
NN |
denotes |
nitrocellulose |
| T11804 |
1602-1611 |
NNS |
denotes |
membranes |
| T11805 |
1611-1612 |
. |
denotes |
. |
| T11806 |
1612-1726 |
sentence |
denotes |
The Western blot was probed with mAb 203–217 followed by incubation with a peroxidase coupled secondary antibody. |
| T11807 |
1613-1616 |
DT |
denotes |
The |
| T11809 |
1617-1624 |
NNP |
denotes |
Western |
| T11808 |
1625-1629 |
NN |
denotes |
blot |
| T11811 |
1630-1633 |
VBD |
denotes |
was |
| T11810 |
1634-1640 |
VBN |
denotes |
probed |
| T11812 |
1641-1645 |
IN |
denotes |
with |
| T11813 |
1646-1649 |
NN |
denotes |
mAb |
| T11814 |
1650-1653 |
CD |
denotes |
203 |
| T11815 |
1653-1654 |
SYM |
denotes |
– |
| T11816 |
1654-1657 |
CD |
denotes |
217 |
| T11817 |
1658-1666 |
VBN |
denotes |
followed |
| T11818 |
1667-1669 |
IN |
denotes |
by |
| T11819 |
1670-1680 |
NN |
denotes |
incubation |
| T11820 |
1681-1685 |
IN |
denotes |
with |
| T11821 |
1686-1687 |
DT |
denotes |
a |
| T11823 |
1688-1698 |
NN |
denotes |
peroxidase |
| T11824 |
1699-1706 |
VBN |
denotes |
coupled |
| T11825 |
1707-1716 |
JJ |
denotes |
secondary |
| T11822 |
1717-1725 |
NN |
denotes |
antibody |
| T11826 |
1725-1726 |
. |
denotes |
. |
| T11827 |
1726-1773 |
sentence |
denotes |
Detection was with enhanced chemiluminescence. |
| T11828 |
1727-1736 |
NN |
denotes |
Detection |
| T11829 |
1737-1740 |
VBD |
denotes |
was |
| T11830 |
1741-1745 |
IN |
denotes |
with |
| T11831 |
1746-1754 |
VBN |
denotes |
enhanced |
| T11832 |
1755-1772 |
NN |
denotes |
chemiluminescence |
| T11833 |
1772-1773 |
. |
denotes |
. |
| T2486 |
1774-1794 |
NN |
denotes |
Immunohistochemistry |
| T2487 |
1795-1804 |
VBD |
denotes |
confirmed |
| T2488 |
1805-1808 |
DT |
denotes |
the |
| T2489 |
1809-1817 |
NN |
denotes |
presence |
| T2490 |
1818-1820 |
IN |
denotes |
of |
| T2491 |
1821-1824 |
DT |
denotes |
the |
| T2492 |
1825-1832 |
NN |
denotes |
protein |
| T2493 |
1833-1839 |
IN |
denotes |
during |
| T2494 |
1840-1845 |
JJ |
denotes |
early |
| T2495 |
1846-1857 |
NN |
denotes |
development |
| T2496 |
1857-1858 |
. |
denotes |
. |
| T2497 |
1858-2095 |
sentence |
denotes |
Annexin A7 immunoreactivity using mAb 203–217, which specifically recognizes both isoforms of Annexin A7, can be observed in the two cell types of the cylinder stage at E5 with a weaker Annexin A7 expression in the ectoderm (Fig. 2A–C). |
| T2498 |
1859-1866 |
NN |
denotes |
Annexin |
| T2499 |
1867-1869 |
NN |
denotes |
A7 |
| T2500 |
1870-1886 |
NN |
denotes |
immunoreactivity |
| T2502 |
1887-1892 |
VBG |
denotes |
using |
| T2503 |
1893-1896 |
NN |
denotes |
mAb |
| T2504 |
1897-1900 |
CD |
denotes |
203 |
| T2505 |
1900-1901 |
SYM |
denotes |
– |
| T2506 |
1901-1904 |
CD |
denotes |
217 |
| T2507 |
1904-1906 |
, |
denotes |
, |
| T2508 |
1906-1911 |
WDT |
denotes |
which |
| T2510 |
1912-1924 |
RB |
denotes |
specifically |
| T2509 |
1925-1935 |
VBZ |
denotes |
recognizes |
| T2511 |
1936-1940 |
DT |
denotes |
both |
| T2512 |
1941-1949 |
NNS |
denotes |
isoforms |
| T2513 |
1950-1952 |
IN |
denotes |
of |
| T2514 |
1953-1960 |
NN |
denotes |
Annexin |
| T2515 |
1961-1963 |
NN |
denotes |
A7 |
| T2516 |
1963-1965 |
, |
denotes |
, |
| T2517 |
1965-1968 |
MD |
denotes |
can |
| T2518 |
1969-1971 |
VB |
denotes |
be |
| T2501 |
1972-1980 |
VBN |
denotes |
observed |
| T2519 |
1981-1983 |
IN |
denotes |
in |
| T2520 |
1984-1987 |
DT |
denotes |
the |
| T2522 |
1988-1991 |
CD |
denotes |
two |
| T2523 |
1992-1996 |
NN |
denotes |
cell |
| T2521 |
1997-2002 |
NNS |
denotes |
types |
| T2524 |
2003-2005 |
IN |
denotes |
of |
| T2525 |
2006-2009 |
DT |
denotes |
the |
| T2527 |
2010-2018 |
NN |
denotes |
cylinder |
| T2526 |
2019-2024 |
NN |
denotes |
stage |
| T2528 |
2025-2027 |
IN |
denotes |
at |
| T2529 |
2028-2030 |
NN |
denotes |
E5 |
| T2530 |
2031-2035 |
IN |
denotes |
with |
| T2531 |
2036-2037 |
DT |
denotes |
a |
| T2533 |
2038-2044 |
JJR |
denotes |
weaker |
| T2534 |
2045-2052 |
NN |
denotes |
Annexin |
| T2535 |
2053-2055 |
NN |
denotes |
A7 |
| T2532 |
2056-2066 |
NN |
denotes |
expression |
| T2536 |
2067-2069 |
IN |
denotes |
in |
| T2537 |
2070-2073 |
DT |
denotes |
the |
| T2538 |
2074-2082 |
NN |
denotes |
ectoderm |
| T2539 |
2083-2084 |
-LRB- |
denotes |
( |
| T2541 |
2084-2088 |
NN |
denotes |
Fig. |
| T2540 |
2089-2091 |
NN |
denotes |
2A |
| T2542 |
2091-2092 |
SYM |
denotes |
– |
| T2543 |
2092-2093 |
NN |
denotes |
C |
| T2544 |
2093-2094 |
-RRB- |
denotes |
) |
| T2545 |
2094-2095 |
. |
denotes |
. |
| T2546 |
2095-2182 |
sentence |
denotes |
At E8 Annexin A7 can be detected in almost all tissues of the embryo (data not shown). |
| T2547 |
2096-2098 |
IN |
denotes |
At |
| T2549 |
2099-2101 |
NN |
denotes |
E8 |
| T2550 |
2102-2109 |
NN |
denotes |
Annexin |
| T2551 |
2110-2112 |
NN |
denotes |
A7 |
| T2552 |
2113-2116 |
MD |
denotes |
can |
| T2553 |
2117-2119 |
VB |
denotes |
be |
| T2548 |
2120-2128 |
VBN |
denotes |
detected |
| T2554 |
2129-2131 |
IN |
denotes |
in |
| T2555 |
2132-2138 |
RB |
denotes |
almost |
| T2557 |
2139-2142 |
DT |
denotes |
all |
| T2556 |
2143-2150 |
NNS |
denotes |
tissues |
| T2558 |
2151-2153 |
IN |
denotes |
of |
| T2559 |
2154-2157 |
DT |
denotes |
the |
| T2560 |
2158-2164 |
NN |
denotes |
embryo |
| T2561 |
2165-2166 |
-LRB- |
denotes |
( |
| T2563 |
2166-2170 |
NNS |
denotes |
data |
| T2564 |
2171-2174 |
RB |
denotes |
not |
| T2562 |
2175-2180 |
VBN |
denotes |
shown |
| T2565 |
2180-2181 |
-RRB- |
denotes |
) |
| T2566 |
2181-2182 |
. |
denotes |
. |
| T2567 |
2182-2312 |
sentence |
denotes |
A correlation between the presence of Annexin A7 and the origin of a cell type from one of the three germ layers is not apparent. |
| T2568 |
2183-2184 |
DT |
denotes |
A |
| T2569 |
2185-2196 |
NN |
denotes |
correlation |
| T2571 |
2197-2204 |
IN |
denotes |
between |
| T2572 |
2205-2208 |
DT |
denotes |
the |
| T2573 |
2209-2217 |
NN |
denotes |
presence |
| T2574 |
2218-2220 |
IN |
denotes |
of |
| T2575 |
2221-2228 |
NN |
denotes |
Annexin |
| T2576 |
2229-2231 |
NN |
denotes |
A7 |
| T2577 |
2232-2235 |
CC |
denotes |
and |
| T2578 |
2236-2239 |
DT |
denotes |
the |
| T2579 |
2240-2246 |
NN |
denotes |
origin |
| T2580 |
2247-2249 |
IN |
denotes |
of |
| T2581 |
2250-2251 |
DT |
denotes |
a |
| T2583 |
2252-2256 |
NN |
denotes |
cell |
| T2582 |
2257-2261 |
NN |
denotes |
type |
| T2584 |
2262-2266 |
IN |
denotes |
from |
| T2585 |
2267-2270 |
CD |
denotes |
one |
| T2587 |
2271-2273 |
IN |
denotes |
of |
| T2588 |
2274-2277 |
DT |
denotes |
the |
| T2586 |
2278-2283 |
CD |
denotes |
three |
| T2590 |
2284-2288 |
NN |
denotes |
germ |
| T2589 |
2289-2295 |
NNS |
denotes |
layers |
| T2570 |
2296-2298 |
VBZ |
denotes |
is |
| T2591 |
2299-2302 |
RB |
denotes |
not |
| T2592 |
2303-2311 |
JJ |
denotes |
apparent |
| T2593 |
2311-2312 |
. |
denotes |
. |
| T2594 |
2312-2468 |
sentence |
denotes |
We specifically noted the localization of the protein in the neuroepithelium of the neural fold and tube (Fig. 2D–F, proximal, E8; Fig. 2G–I, distal, E13). |
| T2595 |
2313-2315 |
PRP |
denotes |
We |
| T2597 |
2316-2328 |
RB |
denotes |
specifically |
| T2596 |
2329-2334 |
VBD |
denotes |
noted |
| T2598 |
2335-2338 |
DT |
denotes |
the |
| T2599 |
2339-2351 |
NN |
denotes |
localization |
| T2600 |
2352-2354 |
IN |
denotes |
of |
| T2601 |
2355-2358 |
DT |
denotes |
the |
| T2602 |
2359-2366 |
NN |
denotes |
protein |
| T2603 |
2367-2369 |
IN |
denotes |
in |
| T2604 |
2370-2373 |
DT |
denotes |
the |
| T2605 |
2374-2389 |
NN |
denotes |
neuroepithelium |
| T2606 |
2390-2392 |
IN |
denotes |
of |
| T2607 |
2393-2396 |
DT |
denotes |
the |
| T2609 |
2397-2403 |
JJ |
denotes |
neural |
| T2608 |
2404-2408 |
NN |
denotes |
fold |
| T2610 |
2409-2412 |
CC |
denotes |
and |
| T2611 |
2413-2417 |
NN |
denotes |
tube |
| T2612 |
2418-2419 |
-LRB- |
denotes |
( |
| T2614 |
2419-2423 |
NN |
denotes |
Fig. |
| T2615 |
2424-2426 |
NN |
denotes |
2D |
| T2617 |
2426-2427 |
SYM |
denotes |
– |
| T2618 |
2427-2428 |
NN |
denotes |
F |
| T2619 |
2428-2430 |
, |
denotes |
, |
| T2620 |
2430-2438 |
JJ |
denotes |
proximal |
| T2621 |
2438-2440 |
, |
denotes |
, |
| T2616 |
2440-2442 |
NN |
denotes |
E8 |
| T2622 |
2442-2443 |
: |
denotes |
; |
| T2623 |
2444-2448 |
NN |
denotes |
Fig. |
| T2624 |
2449-2451 |
NN |
denotes |
2G |
| T2625 |
2451-2452 |
SYM |
denotes |
– |
| T2626 |
2452-2453 |
NN |
denotes |
I |
| T2627 |
2453-2455 |
, |
denotes |
, |
| T2628 |
2455-2461 |
JJ |
denotes |
distal |
| T2629 |
2461-2463 |
, |
denotes |
, |
| T2613 |
2463-2466 |
NN |
denotes |
E13 |
| T2630 |
2466-2467 |
-RRB- |
denotes |
) |
| T2631 |
2467-2468 |
. |
denotes |
. |
| T2632 |
2468-2597 |
sentence |
denotes |
At the subcellular level, Annexin A7 was present in punctate structures mainly in the cytosol of all examined cells (Fig. 2E,F). |
| T2633 |
2469-2471 |
IN |
denotes |
At |
| T2635 |
2472-2475 |
DT |
denotes |
the |
| T2637 |
2476-2487 |
JJ |
denotes |
subcellular |
| T2636 |
2488-2493 |
NN |
denotes |
level |
| T2638 |
2493-2495 |
, |
denotes |
, |
| T2639 |
2495-2502 |
NN |
denotes |
Annexin |
| T2640 |
2503-2505 |
NN |
denotes |
A7 |
| T2634 |
2506-2509 |
VBD |
denotes |
was |
| T2641 |
2510-2517 |
JJ |
denotes |
present |
| T2642 |
2518-2520 |
IN |
denotes |
in |
| T2643 |
2521-2529 |
NN |
denotes |
punctate |
| T2644 |
2530-2540 |
NNS |
denotes |
structures |
| T2645 |
2541-2547 |
RB |
denotes |
mainly |
| T2646 |
2548-2550 |
IN |
denotes |
in |
| T2647 |
2551-2554 |
DT |
denotes |
the |
| T2648 |
2555-2562 |
NN |
denotes |
cytosol |
| T2649 |
2563-2565 |
IN |
denotes |
of |
| T2650 |
2566-2569 |
DT |
denotes |
all |
| T2652 |
2570-2578 |
VBN |
denotes |
examined |
| T2651 |
2579-2584 |
NNS |
denotes |
cells |
| T2653 |
2585-2586 |
-LRB- |
denotes |
( |
| T2655 |
2586-2590 |
NN |
denotes |
Fig. |
| T2656 |
2591-2593 |
NN |
denotes |
2E |
| T2657 |
2593-2594 |
, |
denotes |
, |
| T2654 |
2594-2595 |
NN |
denotes |
F |
| T2658 |
2595-2596 |
-RRB- |
denotes |
) |
| T2659 |
2596-2597 |
. |
denotes |
. |
| T2660 |
2597-2664 |
sentence |
denotes |
This distribution was also observed during subsequent development. |
| T2661 |
2598-2602 |
DT |
denotes |
This |
| T2662 |
2603-2615 |
NN |
denotes |
distribution |
| T2664 |
2616-2619 |
VBD |
denotes |
was |
| T2665 |
2620-2624 |
RB |
denotes |
also |
| T2663 |
2625-2633 |
VBN |
denotes |
observed |
| T2666 |
2634-2640 |
IN |
denotes |
during |
| T2667 |
2641-2651 |
JJ |
denotes |
subsequent |
| T2668 |
2652-2663 |
NN |
denotes |
development |
| T2669 |
2663-2664 |
. |
denotes |
. |
| T12193 |
2675-2682 |
NN |
denotes |
Annexin |
| T12194 |
2683-2685 |
NN |
denotes |
A7 |
| T12195 |
2686-2702 |
NN |
denotes |
immunoreactivity |
| T12196 |
2703-2705 |
IN |
denotes |
in |
| T12197 |
2706-2711 |
JJ |
denotes |
early |
| T12199 |
2712-2717 |
NN |
denotes |
mouse |
| T12198 |
2718-2725 |
NNS |
denotes |
embryos |
| T12200 |
2725-2726 |
. |
denotes |
. |
| T12201 |
2726-2877 |
sentence |
denotes |
(A) Phase contrast, embryo E5: The egg cylinder consists of an inner cell mass (a) representing the ectoderm and an outer layer of endoderm cells (b). |
| T12202 |
2727-2728 |
-LRB- |
denotes |
( |
| T12203 |
2728-2729 |
LS |
denotes |
A |
| T12205 |
2729-2730 |
-RRB- |
denotes |
) |
| T12206 |
2731-2736 |
NN |
denotes |
Phase |
| T12207 |
2737-2745 |
NN |
denotes |
contrast |
| T12208 |
2745-2747 |
, |
denotes |
, |
| T12209 |
2747-2753 |
NN |
denotes |
embryo |
| T12210 |
2754-2756 |
NN |
denotes |
E5 |
| T12211 |
2756-2758 |
: |
denotes |
: |
| T12212 |
2758-2761 |
DT |
denotes |
The |
| T12214 |
2762-2765 |
NN |
denotes |
egg |
| T12213 |
2766-2774 |
NN |
denotes |
cylinder |
| T12204 |
2775-2783 |
VBZ |
denotes |
consists |
| T12215 |
2784-2786 |
IN |
denotes |
of |
| T12216 |
2787-2789 |
DT |
denotes |
an |
| T12218 |
2790-2795 |
JJ |
denotes |
inner |
| T12219 |
2796-2800 |
NN |
denotes |
cell |
| T12217 |
2801-2805 |
NN |
denotes |
mass |
| T12220 |
2806-2807 |
-LRB- |
denotes |
( |
| T12221 |
2807-2808 |
NN |
denotes |
a |
| T12222 |
2808-2809 |
-RRB- |
denotes |
) |
| T12223 |
2810-2822 |
VBG |
denotes |
representing |
| T12224 |
2823-2826 |
DT |
denotes |
the |
| T12225 |
2827-2835 |
NN |
denotes |
ectoderm |
| T12226 |
2836-2839 |
CC |
denotes |
and |
| T12227 |
2840-2842 |
DT |
denotes |
an |
| T12229 |
2843-2848 |
JJ |
denotes |
outer |
| T12228 |
2849-2854 |
NN |
denotes |
layer |
| T12230 |
2855-2857 |
IN |
denotes |
of |
| T12231 |
2858-2866 |
NN |
denotes |
endoderm |
| T12232 |
2867-2872 |
NNS |
denotes |
cells |
| T12233 |
2873-2874 |
-LRB- |
denotes |
( |
| T12234 |
2874-2875 |
NN |
denotes |
b |
| T12235 |
2875-2876 |
-RRB- |
denotes |
) |
| T12236 |
2876-2877 |
. |
denotes |
. |
| T12237 |
2877-2996 |
sentence |
denotes |
(B) Immunostaining of the paraffin section was performed using purified mAb 203–217 and Cy3-conjugated anti-mouse IgG. |
| T12238 |
2878-2879 |
-LRB- |
denotes |
( |
| T12239 |
2879-2880 |
LS |
denotes |
B |
| T12241 |
2880-2881 |
-RRB- |
denotes |
) |
| T12242 |
2882-2896 |
NN |
denotes |
Immunostaining |
| T12243 |
2897-2899 |
IN |
denotes |
of |
| T12244 |
2900-2903 |
DT |
denotes |
the |
| T12246 |
2904-2912 |
NN |
denotes |
paraffin |
| T12245 |
2913-2920 |
NN |
denotes |
section |
| T12247 |
2921-2924 |
VBD |
denotes |
was |
| T12240 |
2925-2934 |
VBN |
denotes |
performed |
| T12248 |
2935-2940 |
VBG |
denotes |
using |
| T12249 |
2941-2949 |
VBN |
denotes |
purified |
| T12250 |
2950-2953 |
NN |
denotes |
mAb |
| T12251 |
2954-2957 |
CD |
denotes |
203 |
| T12252 |
2957-2958 |
SYM |
denotes |
– |
| T12253 |
2958-2961 |
CD |
denotes |
217 |
| T12254 |
2962-2965 |
CC |
denotes |
and |
| T12255 |
2966-2969 |
NN |
denotes |
Cy3 |
| T12257 |
2969-2970 |
HYPH |
denotes |
- |
| T12256 |
2970-2980 |
VBN |
denotes |
conjugated |
| T12259 |
2981-2991 |
JJ |
denotes |
anti-mouse |
| T12258 |
2992-2995 |
NN |
denotes |
IgG |
| T12260 |
2995-2996 |
. |
denotes |
. |
| T12261 |
2996-3137 |
sentence |
denotes |
Annexin A7 is expressed in both cell types of the egg cylinder with a strong staining of the endoderm and a weaker staining of the ectoderm. |
| T12262 |
2997-3004 |
NN |
denotes |
Annexin |
| T12263 |
3005-3007 |
NN |
denotes |
A7 |
| T12265 |
3008-3010 |
VBZ |
denotes |
is |
| T12264 |
3011-3020 |
VBN |
denotes |
expressed |
| T12266 |
3021-3023 |
IN |
denotes |
in |
| T12267 |
3024-3028 |
DT |
denotes |
both |
| T12269 |
3029-3033 |
NN |
denotes |
cell |
| T12268 |
3034-3039 |
NNS |
denotes |
types |
| T12270 |
3040-3042 |
IN |
denotes |
of |
| T12271 |
3043-3046 |
DT |
denotes |
the |
| T12273 |
3047-3050 |
NN |
denotes |
egg |
| T12272 |
3051-3059 |
NN |
denotes |
cylinder |
| T12274 |
3060-3064 |
IN |
denotes |
with |
| T12275 |
3065-3066 |
DT |
denotes |
a |
| T12277 |
3067-3073 |
JJ |
denotes |
strong |
| T12276 |
3074-3082 |
NN |
denotes |
staining |
| T12278 |
3083-3085 |
IN |
denotes |
of |
| T12279 |
3086-3089 |
DT |
denotes |
the |
| T12280 |
3090-3098 |
NN |
denotes |
endoderm |
| T12281 |
3099-3102 |
CC |
denotes |
and |
| T12282 |
3103-3104 |
DT |
denotes |
a |
| T12284 |
3105-3111 |
JJR |
denotes |
weaker |
| T12283 |
3112-3120 |
NN |
denotes |
staining |
| T12285 |
3121-3123 |
IN |
denotes |
of |
| T12286 |
3124-3127 |
DT |
denotes |
the |
| T12287 |
3128-3136 |
NN |
denotes |
ectoderm |
| T12288 |
3136-3137 |
. |
denotes |
. |
| T12289 |
3137-3180 |
sentence |
denotes |
The nuclei are devoid of immune reactions. |
| T12290 |
3138-3141 |
DT |
denotes |
The |
| T12291 |
3142-3148 |
NNS |
denotes |
nuclei |
| T12292 |
3149-3152 |
VBP |
denotes |
are |
| T12293 |
3153-3159 |
JJ |
denotes |
devoid |
| T12294 |
3160-3162 |
IN |
denotes |
of |
| T12295 |
3163-3169 |
JJ |
denotes |
immune |
| T12296 |
3170-3179 |
NNS |
denotes |
reactions |
| T12297 |
3179-3180 |
. |
denotes |
. |
| T12298 |
3180-3240 |
sentence |
denotes |
(C) Negative control using the secondary Cy3-antibody only. |
| T12299 |
3181-3182 |
-LRB- |
denotes |
( |
| T12301 |
3182-3183 |
LS |
denotes |
C |
| T12302 |
3183-3184 |
-RRB- |
denotes |
) |
| T12303 |
3185-3193 |
JJ |
denotes |
Negative |
| T12300 |
3194-3201 |
NN |
denotes |
control |
| T12304 |
3202-3207 |
VBG |
denotes |
using |
| T12305 |
3208-3211 |
DT |
denotes |
the |
| T12307 |
3212-3221 |
JJ |
denotes |
secondary |
| T12308 |
3222-3225 |
NN |
denotes |
Cy3 |
| T12309 |
3225-3226 |
HYPH |
denotes |
- |
| T12306 |
3226-3234 |
NN |
denotes |
antibody |
| T12310 |
3235-3239 |
RB |
denotes |
only |
| T12311 |
3239-3240 |
. |
denotes |
. |
| T12312 |
3240-3361 |
sentence |
denotes |
(D-F) Annexin A7 expression in the proximal neural tube (D) and nearby neural fold (E,F), embryo E8, transverse section. |
| T12313 |
3241-3242 |
-LRB- |
denotes |
( |
| T12314 |
3242-3243 |
LS |
denotes |
D |
| T12316 |
3243-3244 |
SYM |
denotes |
- |
| T12317 |
3244-3245 |
LS |
denotes |
F |
| T12318 |
3245-3246 |
-RRB- |
denotes |
) |
| T12319 |
3247-3254 |
NN |
denotes |
Annexin |
| T12320 |
3255-3257 |
NN |
denotes |
A7 |
| T12321 |
3258-3268 |
NN |
denotes |
expression |
| T12322 |
3269-3271 |
IN |
denotes |
in |
| T12323 |
3272-3275 |
DT |
denotes |
the |
| T12325 |
3276-3284 |
JJ |
denotes |
proximal |
| T12326 |
3285-3291 |
JJ |
denotes |
neural |
| T12324 |
3292-3296 |
NN |
denotes |
tube |
| T12327 |
3297-3298 |
-LRB- |
denotes |
( |
| T12328 |
3298-3299 |
NN |
denotes |
D |
| T12329 |
3299-3300 |
-RRB- |
denotes |
) |
| T12330 |
3301-3304 |
CC |
denotes |
and |
| T12331 |
3305-3311 |
JJ |
denotes |
nearby |
| T12333 |
3312-3318 |
JJ |
denotes |
neural |
| T12332 |
3319-3323 |
NN |
denotes |
fold |
| T12334 |
3324-3325 |
-LRB- |
denotes |
( |
| T12336 |
3325-3326 |
NN |
denotes |
E |
| T12337 |
3326-3327 |
, |
denotes |
, |
| T12335 |
3327-3328 |
NN |
denotes |
F |
| T12338 |
3328-3329 |
-RRB- |
denotes |
) |
| T12339 |
3329-3331 |
, |
denotes |
, |
| T12340 |
3331-3337 |
NN |
denotes |
embryo |
| T12341 |
3338-3340 |
NN |
denotes |
E8 |
| T12342 |
3340-3342 |
, |
denotes |
, |
| T12343 |
3342-3352 |
JJ |
denotes |
transverse |
| T12315 |
3353-3360 |
NN |
denotes |
section |
| T12344 |
3360-3361 |
. |
denotes |
. |
| T12345 |
3361-3503 |
sentence |
denotes |
Immunolabeling of Annexin A7 was performed with purified mAb 203–217 and visualization was with an Alexa Fluor 488-conjugated anti-mouse IgG. |
| T12346 |
3362-3376 |
NN |
denotes |
Immunolabeling |
| T12348 |
3377-3379 |
IN |
denotes |
of |
| T12349 |
3380-3387 |
NN |
denotes |
Annexin |
| T12350 |
3388-3390 |
NN |
denotes |
A7 |
| T12351 |
3391-3394 |
VBD |
denotes |
was |
| T12347 |
3395-3404 |
VBN |
denotes |
performed |
| T12352 |
3405-3409 |
IN |
denotes |
with |
| T12353 |
3410-3418 |
VBN |
denotes |
purified |
| T12355 |
3419-3422 |
NN |
denotes |
mAb |
| T12354 |
3423-3426 |
CD |
denotes |
203 |
| T12356 |
3426-3427 |
SYM |
denotes |
– |
| T12357 |
3427-3430 |
CD |
denotes |
217 |
| T12358 |
3431-3434 |
CC |
denotes |
and |
| T12359 |
3435-3448 |
NN |
denotes |
visualization |
| T12360 |
3449-3452 |
VBD |
denotes |
was |
| T12361 |
3453-3457 |
IN |
denotes |
with |
| T12362 |
3458-3460 |
DT |
denotes |
an |
| T12364 |
3461-3466 |
NNP |
denotes |
Alexa |
| T12365 |
3467-3472 |
NNP |
denotes |
Fluor |
| T12367 |
3473-3476 |
CD |
denotes |
488 |
| T12368 |
3476-3477 |
HYPH |
denotes |
- |
| T12366 |
3477-3487 |
VBN |
denotes |
conjugated |
| T12369 |
3488-3498 |
JJ |
denotes |
anti-mouse |
| T12363 |
3499-3502 |
NN |
denotes |
IgG |
| T12370 |
3502-3503 |
. |
denotes |
. |
| T12371 |
3503-3627 |
sentence |
denotes |
(D) An intense Annexin A7 immunostaining is detectable in the neuroepithelium of the neural tube (a, lumen of neural tube). |
| T12372 |
3504-3505 |
-LRB- |
denotes |
( |
| T12373 |
3505-3506 |
LS |
denotes |
D |
| T12375 |
3506-3507 |
-RRB- |
denotes |
) |
| T12376 |
3508-3510 |
DT |
denotes |
An |
| T12378 |
3511-3518 |
JJ |
denotes |
intense |
| T12379 |
3519-3526 |
NN |
denotes |
Annexin |
| T12380 |
3527-3529 |
NN |
denotes |
A7 |
| T12377 |
3530-3544 |
NN |
denotes |
immunostaining |
| T12374 |
3545-3547 |
VBZ |
denotes |
is |
| T12381 |
3548-3558 |
JJ |
denotes |
detectable |
| T12382 |
3559-3561 |
IN |
denotes |
in |
| T12383 |
3562-3565 |
DT |
denotes |
the |
| T12384 |
3566-3581 |
NN |
denotes |
neuroepithelium |
| T12385 |
3582-3584 |
IN |
denotes |
of |
| T12386 |
3585-3588 |
DT |
denotes |
the |
| T12388 |
3589-3595 |
JJ |
denotes |
neural |
| T12387 |
3596-3600 |
NN |
denotes |
tube |
| T12389 |
3601-3602 |
-LRB- |
denotes |
( |
| T12391 |
3602-3603 |
NN |
denotes |
a |
| T12392 |
3603-3605 |
, |
denotes |
, |
| T12390 |
3605-3610 |
NN |
denotes |
lumen |
| T12393 |
3611-3613 |
IN |
denotes |
of |
| T12394 |
3614-3620 |
JJ |
denotes |
neural |
| T12395 |
3621-3625 |
NN |
denotes |
tube |
| T12396 |
3625-3626 |
-RRB- |
denotes |
) |
| T12397 |
3626-3627 |
. |
denotes |
. |
| T12398 |
3627-3728 |
sentence |
denotes |
(E,F) Higher magnifications of the neuroepithelium show that Annexin A7 is expressed in the cytosol. |
| T12399 |
3628-3629 |
-LRB- |
denotes |
( |
| T12400 |
3629-3630 |
LS |
denotes |
E |
| T12402 |
3630-3631 |
, |
denotes |
, |
| T12403 |
3631-3632 |
LS |
denotes |
F |
| T12404 |
3632-3633 |
-RRB- |
denotes |
) |
| T12405 |
3634-3640 |
JJR |
denotes |
Higher |
| T12406 |
3641-3655 |
NNS |
denotes |
magnifications |
| T12407 |
3656-3658 |
IN |
denotes |
of |
| T12408 |
3659-3662 |
DT |
denotes |
the |
| T12409 |
3663-3678 |
NN |
denotes |
neuroepithelium |
| T12401 |
3679-3683 |
VBP |
denotes |
show |
| T12410 |
3684-3688 |
IN |
denotes |
that |
| T12412 |
3689-3696 |
NN |
denotes |
Annexin |
| T12413 |
3697-3699 |
NN |
denotes |
A7 |
| T12414 |
3700-3702 |
VBZ |
denotes |
is |
| T12411 |
3703-3712 |
VBN |
denotes |
expressed |
| T12415 |
3713-3715 |
IN |
denotes |
in |
| T12416 |
3716-3719 |
DT |
denotes |
the |
| T12417 |
3720-3727 |
NN |
denotes |
cytosol |
| T12418 |
3727-3728 |
. |
denotes |
. |
| T12419 |
3728-3792 |
sentence |
denotes |
Arrowheads point to Annexin A7 immunoreactivity in the cytosol. |
| T12420 |
3729-3739 |
NNS |
denotes |
Arrowheads |
| T12421 |
3740-3745 |
VBP |
denotes |
point |
| T12422 |
3746-3748 |
IN |
denotes |
to |
| T12423 |
3749-3756 |
NN |
denotes |
Annexin |
| T12424 |
3757-3759 |
NN |
denotes |
A7 |
| T12425 |
3760-3776 |
NN |
denotes |
immunoreactivity |
| T12426 |
3777-3779 |
IN |
denotes |
in |
| T12427 |
3780-3783 |
DT |
denotes |
the |
| T12428 |
3784-3791 |
NN |
denotes |
cytosol |
| T12429 |
3791-3792 |
. |
denotes |
. |
| T12430 |
3792-3844 |
sentence |
denotes |
(G) Phase contrast, embryo E13, caudal neural tube. |
| T12431 |
3793-3794 |
-LRB- |
denotes |
( |
| T12433 |
3794-3795 |
LS |
denotes |
G |
| T12434 |
3795-3796 |
-RRB- |
denotes |
) |
| T12435 |
3797-3802 |
NN |
denotes |
Phase |
| T12436 |
3803-3811 |
NN |
denotes |
contrast |
| T12437 |
3811-3813 |
, |
denotes |
, |
| T12438 |
3813-3819 |
NN |
denotes |
embryo |
| T12439 |
3820-3823 |
NN |
denotes |
E13 |
| T12440 |
3823-3825 |
, |
denotes |
, |
| T12441 |
3825-3831 |
JJ |
denotes |
caudal |
| T12442 |
3832-3838 |
JJ |
denotes |
neural |
| T12432 |
3839-3843 |
NN |
denotes |
tube |
| T12443 |
3843-3844 |
. |
denotes |
. |
| T12444 |
3844-3975 |
sentence |
denotes |
(H) Immunostaining of the paraffin section was performed using purified mAb 203–217 and Alexa Fluor 488-conjugated anti-mouse IgG. |
| T12445 |
3845-3846 |
-LRB- |
denotes |
( |
| T12446 |
3846-3847 |
LS |
denotes |
H |
| T12448 |
3847-3848 |
-RRB- |
denotes |
) |
| T12449 |
3849-3863 |
NN |
denotes |
Immunostaining |
| T12450 |
3864-3866 |
IN |
denotes |
of |
| T12451 |
3867-3870 |
DT |
denotes |
the |
| T12453 |
3871-3879 |
NN |
denotes |
paraffin |
| T12452 |
3880-3887 |
NN |
denotes |
section |
| T12454 |
3888-3891 |
VBD |
denotes |
was |
| T12447 |
3892-3901 |
VBN |
denotes |
performed |
| T12455 |
3902-3907 |
VBG |
denotes |
using |
| T12456 |
3908-3916 |
VBN |
denotes |
purified |
| T12458 |
3917-3920 |
NN |
denotes |
mAb |
| T12457 |
3921-3924 |
CD |
denotes |
203 |
| T12459 |
3924-3925 |
SYM |
denotes |
– |
| T12460 |
3925-3928 |
CD |
denotes |
217 |
| T12461 |
3929-3932 |
CC |
denotes |
and |
| T12462 |
3933-3938 |
NNP |
denotes |
Alexa |
| T12463 |
3939-3944 |
NNP |
denotes |
Fluor |
| T12465 |
3945-3948 |
CD |
denotes |
488 |
| T12466 |
3948-3949 |
HYPH |
denotes |
- |
| T12464 |
3949-3959 |
VBN |
denotes |
conjugated |
| T12468 |
3960-3970 |
JJ |
denotes |
anti-mouse |
| T12467 |
3971-3974 |
NN |
denotes |
IgG |
| T12469 |
3974-3975 |
. |
denotes |
. |
| T12470 |
3975-4047 |
sentence |
denotes |
(I) Negative control using the secondary Alexa Fluor 488-antibody only. |
| T12471 |
3976-3977 |
-LRB- |
denotes |
( |
| T12472 |
3977-3978 |
LS |
denotes |
I |
| T12474 |
3978-3979 |
-RRB- |
denotes |
) |
| T12475 |
3980-3988 |
JJ |
denotes |
Negative |
| T12473 |
3989-3996 |
NN |
denotes |
control |
| T12476 |
3997-4002 |
VBG |
denotes |
using |
| T12477 |
4003-4006 |
DT |
denotes |
the |
| T12479 |
4007-4016 |
JJ |
denotes |
secondary |
| T12480 |
4017-4022 |
NNP |
denotes |
Alexa |
| T12481 |
4023-4028 |
NNP |
denotes |
Fluor |
| T12482 |
4029-4032 |
CD |
denotes |
488 |
| T12483 |
4032-4033 |
HYPH |
denotes |
- |
| T12478 |
4033-4041 |
NN |
denotes |
antibody |
| T12484 |
4042-4046 |
RB |
denotes |
only |
| T12485 |
4046-4047 |
. |
denotes |
. |
| T12486 |
4047-4059 |
sentence |
denotes |
Bar, 20 μm. |
| T12487 |
4048-4051 |
NN |
denotes |
Bar |
| T12488 |
4051-4053 |
, |
denotes |
, |
| T12489 |
4053-4055 |
CD |
denotes |
20 |
| T12490 |
4056-4058 |
NN |
denotes |
μm |
| T12491 |
4058-4059 |
. |
denotes |
. |
| R1000 |
T2533 |
T2532 |
amod |
weaker,expression |
| R1001 |
T2534 |
T2535 |
compound |
Annexin,A7 |
| R1002 |
T2535 |
T2532 |
compound |
A7,expression |
| R1003 |
T2536 |
T2532 |
prep |
in,expression |
| R1004 |
T2537 |
T2538 |
det |
the,ectoderm |
| R1005 |
T2538 |
T2536 |
pobj |
ectoderm,in |
| R1006 |
T2539 |
T2540 |
punct |
(,2A |
| R1007 |
T2540 |
T2501 |
parataxis |
2A,observed |
| R1008 |
T2541 |
T2540 |
compound |
Fig.,2A |
| R1009 |
T2542 |
T2543 |
punct |
–,C |
| R1010 |
T2543 |
T2540 |
prep |
C,2A |
| R1011 |
T2544 |
T2540 |
punct |
),2A |
| R1012 |
T2545 |
T2501 |
punct |
.,observed |
| R1013 |
T2547 |
T2548 |
prep |
At,detected |
| R1014 |
T2549 |
T2547 |
pobj |
E8,At |
| R1015 |
T2550 |
T2551 |
compound |
Annexin,A7 |
| R1016 |
T2551 |
T2548 |
nsubjpass |
A7,detected |
| R1017 |
T2552 |
T2548 |
aux |
can,detected |
| R1018 |
T2553 |
T2548 |
auxpass |
be,detected |
| R1019 |
T2554 |
T2548 |
prep |
in,detected |
| R1020 |
T2555 |
T2556 |
advmod |
almost,tissues |
| R1021 |
T2556 |
T2554 |
pobj |
tissues,in |
| R1022 |
T2557 |
T2556 |
det |
all,tissues |
| R1023 |
T2558 |
T2556 |
prep |
of,tissues |
| R1024 |
T2559 |
T2560 |
det |
the,embryo |
| R1025 |
T2560 |
T2558 |
pobj |
embryo,of |
| R1026 |
T2561 |
T2562 |
punct |
(,shown |
| R1027 |
T2562 |
T2548 |
parataxis |
shown,detected |
| R1028 |
T2563 |
T2562 |
nsubj |
data,shown |
| R1029 |
T2564 |
T2562 |
neg |
not,shown |
| R1030 |
T2565 |
T2562 |
punct |
),shown |
| R1031 |
T2566 |
T2548 |
punct |
.,detected |
| R1032 |
T2568 |
T2569 |
det |
A,correlation |
| R1033 |
T2569 |
T2570 |
nsubj |
correlation,is |
| R1034 |
T2571 |
T2569 |
prep |
between,correlation |
| R1035 |
T2572 |
T2573 |
det |
the,presence |
| R1036 |
T2573 |
T2571 |
pobj |
presence,between |
| R1037 |
T2574 |
T2573 |
prep |
of,presence |
| R1038 |
T2575 |
T2576 |
compound |
Annexin,A7 |
| R1039 |
T2576 |
T2574 |
pobj |
A7,of |
| R1040 |
T2577 |
T2573 |
cc |
and,presence |
| R1041 |
T2578 |
T2579 |
det |
the,origin |
| R1042 |
T2579 |
T2573 |
conj |
origin,presence |
| R1043 |
T2580 |
T2579 |
prep |
of,origin |
| R1044 |
T2581 |
T2582 |
det |
a,type |
| R1045 |
T2582 |
T2580 |
pobj |
type,of |
| R1046 |
T2583 |
T2582 |
compound |
cell,type |
| R1047 |
T2584 |
T2582 |
prep |
from,type |
| R1048 |
T2585 |
T2586 |
quantmod |
one,three |
| R1049 |
T2586 |
T2589 |
nummod |
three,layers |
| R1050 |
T2587 |
T2586 |
quantmod |
of,three |
| R1051 |
T2588 |
T2586 |
quantmod |
the,three |
| R1052 |
T2589 |
T2584 |
pobj |
layers,from |
| R1053 |
T2590 |
T2589 |
compound |
germ,layers |
| R1054 |
T2591 |
T2570 |
neg |
not,is |
| R1055 |
T2592 |
T2570 |
acomp |
apparent,is |
| R1056 |
T2593 |
T2570 |
punct |
.,is |
| R1057 |
T2595 |
T2596 |
nsubj |
We,noted |
| R1058 |
T2597 |
T2596 |
advmod |
specifically,noted |
| R1059 |
T2598 |
T2599 |
det |
the,localization |
| R1060 |
T2599 |
T2596 |
dobj |
localization,noted |
| R1061 |
T2600 |
T2599 |
prep |
of,localization |
| R1062 |
T2601 |
T2602 |
det |
the,protein |
| R1063 |
T2602 |
T2600 |
pobj |
protein,of |
| R1064 |
T2603 |
T2596 |
prep |
in,noted |
| R1065 |
T2604 |
T2605 |
det |
the,neuroepithelium |
| R1066 |
T2605 |
T2603 |
pobj |
neuroepithelium,in |
| R1067 |
T2606 |
T2605 |
prep |
of,neuroepithelium |
| R1068 |
T2607 |
T2608 |
det |
the,fold |
| R1069 |
T2608 |
T2606 |
pobj |
fold,of |
| R1070 |
T2609 |
T2608 |
amod |
neural,fold |
| R1071 |
T2610 |
T2608 |
cc |
and,fold |
| R1072 |
T2611 |
T2608 |
conj |
tube,fold |
| R1073 |
T2612 |
T2613 |
punct |
(,E13 |
| R1074 |
T2613 |
T2596 |
parataxis |
E13,noted |
| R1075 |
T2614 |
T2615 |
compound |
Fig.,2D |
| R1076 |
T2615 |
T2616 |
dep |
2D,E8 |
| R1077 |
T2616 |
T2613 |
dep |
E8,E13 |
| R1078 |
T2617 |
T2618 |
punct |
–,F |
| R1079 |
T2618 |
T2615 |
prep |
F,2D |
| R1080 |
T2619 |
T2616 |
punct |
", ",E8 |
| R1081 |
T2620 |
T2616 |
amod |
proximal,E8 |
| R1082 |
T2621 |
T2616 |
punct |
", ",E8 |
| R1083 |
T2622 |
T2613 |
punct |
;,E13 |
| R1084 |
T2623 |
T2624 |
compound |
Fig.,2G |
| R1085 |
T2624 |
T2613 |
dep |
2G,E13 |
| R1086 |
T2625 |
T2626 |
punct |
–,I |
| R1087 |
T2626 |
T2624 |
prep |
I,2G |
| R1088 |
T2627 |
T2613 |
punct |
", ",E13 |
| R1089 |
T2628 |
T2613 |
amod |
distal,E13 |
| R1090 |
T2629 |
T2613 |
punct |
", ",E13 |
| R1091 |
T2630 |
T2613 |
punct |
),E13 |
| R1092 |
T2631 |
T2596 |
punct |
.,noted |
| R1093 |
T2633 |
T2634 |
prep |
At,was |
| R1094 |
T2635 |
T2636 |
det |
the,level |
| R1095 |
T2636 |
T2633 |
pobj |
level,At |
| R1096 |
T2637 |
T2636 |
amod |
subcellular,level |
| R1097 |
T2638 |
T2634 |
punct |
", ",was |
| R1098 |
T2639 |
T2640 |
compound |
Annexin,A7 |
| R1099 |
T2640 |
T2634 |
nsubj |
A7,was |
| R1100 |
T2641 |
T2634 |
acomp |
present,was |
| R1101 |
T2642 |
T2634 |
prep |
in,was |
| R1102 |
T2643 |
T2644 |
compound |
punctate,structures |
| R1103 |
T2644 |
T2642 |
pobj |
structures,in |
| R1104 |
T2645 |
T2646 |
advmod |
mainly,in |
| R1105 |
T2646 |
T2634 |
prep |
in,was |
| R1106 |
T2647 |
T2648 |
det |
the,cytosol |
| R1107 |
T2648 |
T2646 |
pobj |
cytosol,in |
| R1108 |
T2649 |
T2648 |
prep |
of,cytosol |
| R1109 |
T2650 |
T2651 |
det |
all,cells |
| R1110 |
T2651 |
T2649 |
pobj |
cells,of |
| R1111 |
T2652 |
T2651 |
amod |
examined,cells |
| R1112 |
T2653 |
T2654 |
punct |
(,F |
| R1113 |
T2654 |
T2634 |
parataxis |
F,was |
| R1114 |
T2655 |
T2654 |
nmod |
Fig.,F |
| R1115 |
T2656 |
T2654 |
nmod |
2E,F |
| R1116 |
T2657 |
T2654 |
punct |
",",F |
| R1117 |
T2658 |
T2654 |
punct |
),F |
| R1118 |
T2659 |
T2634 |
punct |
.,was |
| R1119 |
T2661 |
T2662 |
det |
This,distribution |
| R1120 |
T2662 |
T2663 |
nsubjpass |
distribution,observed |
| R1121 |
T2664 |
T2663 |
auxpass |
was,observed |
| R1122 |
T2665 |
T2663 |
advmod |
also,observed |
| R1123 |
T2666 |
T2663 |
prep |
during,observed |
| R1124 |
T2667 |
T2668 |
amod |
subsequent,development |
| R1125 |
T2668 |
T2666 |
pobj |
development,during |
| R1126 |
T2669 |
T2663 |
punct |
.,observed |
| R5559 |
T11655 |
T11654 |
prep |
of,Expression |
| R5560 |
T11656 |
T11657 |
compound |
Annexin,A7 |
| R5561 |
T11657 |
T11655 |
pobj |
A7,of |
| R5562 |
T11658 |
T11654 |
punct |
.,Expression |
| R5563 |
T11660 |
T11661 |
punct |
(,A |
| R5564 |
T11661 |
T11662 |
meta |
A,analysis |
| R5565 |
T11663 |
T11661 |
punct |
),A |
| R5566 |
T11664 |
T11665 |
compound |
Northern,blot |
| R5567 |
T11665 |
T11662 |
compound |
blot,analysis |
| R5568 |
T11666 |
T11662 |
prep |
of,analysis |
| R5569 |
T11667 |
T11666 |
pobj |
RNA,of |
| R5570 |
T11668 |
T11667 |
prep |
from,RNA |
| R5571 |
T11669 |
T11670 |
compound |
ES,cells |
| R5572 |
T11670 |
T11668 |
pobj |
cells,from |
| R5573 |
T11671 |
T11670 |
punct |
-,cells |
| R5574 |
T11672 |
T11670 |
cc |
and,cells |
| R5575 |
T11673 |
T11674 |
nmod |
embryos,E17 |
| R5576 |
T11674 |
T11670 |
conj |
E17,cells |
| R5577 |
T11675 |
T11674 |
nmod |
E7,E17 |
| R5578 |
T11676 |
T11674 |
punct |
", ",E17 |
| R5579 |
T11677 |
T11674 |
nmod |
E11,E17 |
| R5580 |
T11678 |
T11674 |
punct |
", ",E17 |
| R5581 |
T11679 |
T11674 |
nmod |
E15,E17 |
| R5582 |
T11680 |
T11674 |
punct |
", ",E17 |
| R5583 |
T11681 |
T11667 |
acl |
probed,RNA |
| R5584 |
T11682 |
T11681 |
prep |
with,probed |
| R5585 |
T11683 |
T11684 |
det |
a,cDNA |
| R5586 |
T11684 |
T11682 |
pobj |
cDNA,with |
| R5587 |
T11685 |
T11686 |
amod |
full,length |
| R5588 |
T11686 |
T11684 |
compound |
length,cDNA |
| R5589 |
T11687 |
T11688 |
compound |
annexin,A7 |
| R5590 |
T11688 |
T11684 |
compound |
A7,cDNA |
| R5591 |
T11689 |
T11662 |
punct |
.,analysis |
| R5592 |
T11691 |
T11692 |
nsubjpass |
Transcripts,detected |
| R5593 |
T11693 |
T11691 |
prep |
of,Transcripts |
| R5594 |
T11694 |
T11695 |
nummod |
1.8,kb |
| R5595 |
T11695 |
T11693 |
pobj |
kb,of |
| R5596 |
T11696 |
T11694 |
cc |
and,1.8 |
| R5597 |
T11697 |
T11694 |
conj |
2.4,1.8 |
| R5598 |
T11698 |
T11692 |
auxpass |
are,detected |
| R5599 |
T11699 |
T11692 |
prep |
in,detected |
| R5600 |
T11700 |
T11701 |
det |
all,stages |
| R5601 |
T11701 |
T11699 |
pobj |
stages,in |
| R5602 |
T11702 |
T11701 |
acl |
examined,stages |
| R5603 |
T11703 |
T11704 |
punct |
(,panel |
| R5604 |
T11704 |
T11692 |
parataxis |
panel,detected |
| R5605 |
T11705 |
T11704 |
amod |
upper,panel |
| R5606 |
T11706 |
T11704 |
punct |
),panel |
| R5607 |
T11707 |
T11692 |
punct |
.,detected |
| R5608 |
T11709 |
T11710 |
nsubj |
Reprobing,verified |
| R5609 |
T11711 |
T11709 |
prep |
with,Reprobing |
| R5610 |
T11712 |
T11713 |
det |
a,probe |
| R5611 |
T11713 |
T11711 |
pobj |
probe,with |
| R5612 |
T11714 |
T11715 |
compound |
β,actin |
| R5613 |
T11715 |
T11713 |
compound |
actin,probe |
| R5614 |
T11716 |
T11715 |
punct |
-,actin |
| R5615 |
T11717 |
T11718 |
amod |
equal,loading |
| R5616 |
T11718 |
T11710 |
dobj |
loading,verified |
| R5617 |
T11719 |
T11720 |
punct |
(,band |
| R5618 |
T11720 |
T11710 |
parataxis |
band,verified |
| R5619 |
T11721 |
T11722 |
amod |
lower,panel |
| R5620 |
T11722 |
T11720 |
dep |
panel,band |
| R5621 |
T11723 |
T11720 |
punct |
", ",band |
| R5622 |
T11724 |
T11725 |
nummod |
2.0,kb |
| R5623 |
T11725 |
T11720 |
compound |
kb,band |
| R5624 |
T11726 |
T11720 |
punct |
),band |
| R5625 |
T11727 |
T11710 |
punct |
.,verified |
| R5626 |
T11729 |
T11730 |
punct |
(,B |
| R5627 |
T11730 |
T11731 |
meta |
B,express |
| R5628 |
T11732 |
T11730 |
punct |
),B |
| R5629 |
T11733 |
T11734 |
amod |
Murine,cells |
| R5630 |
T11734 |
T11731 |
nsubj |
cells,express |
| R5631 |
T11735 |
T11734 |
compound |
ES,cells |
| R5632 |
T11736 |
T11734 |
punct |
(,cells |
| R5633 |
T11737 |
T11734 |
appos |
ES,cells |
| R5634 |
T11738 |
T11731 |
punct |
),express |
| R5635 |
T11739 |
T11740 |
advmod |
only,isoform |
| R5636 |
T11740 |
T11731 |
dobj |
isoform,express |
| R5637 |
T11741 |
T11740 |
det |
the,isoform |
| R5638 |
T11742 |
T11740 |
amod |
small,isoform |
| R5639 |
T11743 |
T11744 |
compound |
Annexin,A7 |
| R5640 |
T11744 |
T11740 |
compound |
A7,isoform |
| R5641 |
T11745 |
T11731 |
punct |
.,express |
| R5642 |
T11747 |
T11748 |
det |
Both,isoforms |
| R5643 |
T11748 |
T11749 |
nsubjpass |
isoforms,expressed |
| R5644 |
T11750 |
T11749 |
auxpass |
are,expressed |
| R5645 |
T11751 |
T11749 |
prep |
in,expressed |
| R5646 |
T11752 |
T11753 |
compound |
neuroblastoma,cells |
| R5647 |
T11753 |
T11751 |
pobj |
cells,in |
| R5648 |
T11754 |
T11755 |
compound |
neuro,2a |
| R5649 |
T11755 |
T11753 |
compound |
2a,cells |
| R5650 |
T11756 |
T11755 |
punct |
-,2a |
| R5651 |
T11757 |
T11753 |
punct |
(,cells |
| R5652 |
T11758 |
T11753 |
appos |
N2A,cells |
| R5653 |
T11759 |
T11753 |
punct |
),cells |
| R5654 |
T11760 |
T11753 |
punct |
", ",cells |
| R5655 |
T11761 |
T11753 |
conj |
heart,cells |
| R5656 |
T11762 |
T11761 |
punct |
(,heart |
| R5657 |
T11763 |
T11761 |
appos |
HRT,heart |
| R5658 |
T11764 |
T11761 |
punct |
),heart |
| R5659 |
T11765 |
T11761 |
cc |
and,heart |
| R5660 |
T11766 |
T11767 |
compound |
brain,tissue |
| R5661 |
T11767 |
T11761 |
conj |
tissue,heart |
| R5662 |
T11768 |
T11767 |
punct |
(,tissue |
| R5663 |
T11769 |
T11767 |
appos |
CNS,tissue |
| R5664 |
T11770 |
T11753 |
punct |
),cells |
| R5665 |
T11771 |
T11753 |
prep |
from,cells |
| R5666 |
T11772 |
T11773 |
amod |
adult,mice |
| R5667 |
T11773 |
T11771 |
pobj |
mice,from |
| R5668 |
T11774 |
T11749 |
punct |
.,expressed |
| R5669 |
T11776 |
T11777 |
nsubjpass |
Proteins,resolved |
| R5670 |
T11778 |
T11776 |
prep |
from,Proteins |
| R5671 |
T11779 |
T11780 |
compound |
neuro,2a |
| R5672 |
T11780 |
T11778 |
pobj |
2a,from |
| R5673 |
T11781 |
T11780 |
punct |
-,2a |
| R5674 |
T11782 |
T11780 |
cc |
and,2a |
| R5675 |
T11783 |
T11780 |
conj |
ES,2a |
| R5676 |
T11784 |
T11780 |
appos |
cells,2a |
| R5677 |
T11785 |
T11784 |
punct |
", ",cells |
| R5678 |
T11786 |
T11784 |
conj |
heart,cells |
| R5679 |
T11787 |
T11786 |
cc |
and,heart |
| R5680 |
T11788 |
T11786 |
conj |
brain,heart |
| R5681 |
T11789 |
T11777 |
auxpass |
were,resolved |
| R5682 |
T11790 |
T11777 |
prep |
by,resolved |
| R5683 |
T11791 |
T11792 |
compound |
SDS,electrophoresis |
| R5684 |
T11792 |
T11790 |
pobj |
electrophoresis,by |
| R5685 |
T11793 |
T11794 |
compound |
polyacrylamide,gel |
| R5686 |
T11794 |
T11792 |
compound |
gel,electrophoresis |
| R5687 |
T11795 |
T11796 |
punct |
(,acrylamide |
| R5688 |
T11796 |
T11777 |
parataxis |
acrylamide,resolved |
| R5689 |
T11797 |
T11798 |
nummod |
12,% |
| R5690 |
T11798 |
T11796 |
compound |
%,acrylamide |
| R5691 |
T11799 |
T11796 |
punct |
),acrylamide |
| R5692 |
T11800 |
T11777 |
cc |
and,resolved |
| R5693 |
T11801 |
T11777 |
conj |
transferred,resolved |
| R5694 |
T11802 |
T11801 |
prep |
to,transferred |
| R5695 |
T11803 |
T11804 |
compound |
nitrocellulose,membranes |
| R5696 |
T11804 |
T11802 |
pobj |
membranes,to |
| R5697 |
T11805 |
T11777 |
punct |
.,resolved |
| R5698 |
T11807 |
T11808 |
det |
The,blot |
| R5699 |
T11808 |
T11810 |
nsubjpass |
blot,probed |
| R5700 |
T11809 |
T11808 |
compound |
Western,blot |
| R5701 |
T11811 |
T11810 |
auxpass |
was,probed |
| R5702 |
T11812 |
T11810 |
prep |
with,probed |
| R5703 |
T11813 |
T11814 |
nmod |
mAb,203 |
| R5704 |
T11814 |
T11812 |
pobj |
203,with |
| R5705 |
T11815 |
T11816 |
punct |
–,217 |
| R5706 |
T11816 |
T11814 |
prep |
217,203 |
| R5707 |
T11817 |
T11814 |
acl |
followed,203 |
| R5708 |
T11818 |
T11817 |
agent |
by,followed |
| R5709 |
T11819 |
T11818 |
pobj |
incubation,by |
| R5710 |
T11820 |
T11819 |
prep |
with,incubation |
| R5711 |
T11821 |
T11822 |
det |
a,antibody |
| R5712 |
T11822 |
T11820 |
pobj |
antibody,with |
| R5713 |
T11823 |
T11824 |
npadvmod |
peroxidase,coupled |
| R5714 |
T11824 |
T11822 |
amod |
coupled,antibody |
| R5715 |
T11825 |
T11822 |
amod |
secondary,antibody |
| R5716 |
T11826 |
T11810 |
punct |
.,probed |
| R5717 |
T11828 |
T11829 |
nsubj |
Detection,was |
| R5718 |
T11830 |
T11829 |
prep |
with,was |
| R5719 |
T11831 |
T11832 |
amod |
enhanced,chemiluminescence |
| R5720 |
T11832 |
T11830 |
pobj |
chemiluminescence,with |
| R5721 |
T11833 |
T11829 |
punct |
.,was |
| R5724 |
T12193 |
T12194 |
compound |
Annexin,A7 |
| R5725 |
T12194 |
T12195 |
compound |
A7,immunoreactivity |
| R5726 |
T12196 |
T12195 |
prep |
in,immunoreactivity |
| R5727 |
T12197 |
T12198 |
amod |
early,embryos |
| R5728 |
T12198 |
T12196 |
pobj |
embryos,in |
| R5729 |
T12199 |
T12198 |
compound |
mouse,embryos |
| R5730 |
T12200 |
T12195 |
punct |
.,immunoreactivity |
| R5731 |
T12202 |
T12203 |
punct |
(,A |
| R5732 |
T12203 |
T12204 |
meta |
A,consists |
| R5733 |
T12205 |
T12203 |
punct |
),A |
| R5734 |
T12206 |
T12207 |
compound |
Phase,contrast |
| R5735 |
T12207 |
T12204 |
dep |
contrast,consists |
| R5736 |
T12208 |
T12204 |
punct |
", ",consists |
| R5737 |
T12209 |
T12210 |
compound |
embryo,E5 |
| R5738 |
T12210 |
T12204 |
dep |
E5,consists |
| R5739 |
T12211 |
T12204 |
punct |
: ,consists |
| R5740 |
T12212 |
T12213 |
det |
The,cylinder |
| R5741 |
T12213 |
T12204 |
nsubj |
cylinder,consists |
| R5742 |
T12214 |
T12213 |
compound |
egg,cylinder |
| R5743 |
T12215 |
T12204 |
prep |
of,consists |
| R5744 |
T12216 |
T12217 |
det |
an,mass |
| R5745 |
T12217 |
T12215 |
pobj |
mass,of |
| R5746 |
T12218 |
T12217 |
amod |
inner,mass |
| R5747 |
T12219 |
T12217 |
compound |
cell,mass |
| R5748 |
T12220 |
T12221 |
punct |
(,a |
| R5749 |
T12221 |
T12217 |
parataxis |
a,mass |
| R5750 |
T12222 |
T12221 |
punct |
),a |
| R5751 |
T12223 |
T12217 |
acl |
representing,mass |
| R5752 |
T12224 |
T12225 |
det |
the,ectoderm |
| R5753 |
T12225 |
T12223 |
dobj |
ectoderm,representing |
| R5754 |
T12226 |
T12217 |
cc |
and,mass |
| R5755 |
T12227 |
T12228 |
det |
an,layer |
| R5756 |
T12228 |
T12217 |
conj |
layer,mass |
| R5757 |
T12229 |
T12228 |
amod |
outer,layer |
| R5758 |
T12230 |
T12228 |
prep |
of,layer |
| R5759 |
T12231 |
T12232 |
compound |
endoderm,cells |
| R5760 |
T12232 |
T12230 |
pobj |
cells,of |
| R5761 |
T12233 |
T12234 |
punct |
(,b |
| R5762 |
T12234 |
T12228 |
parataxis |
b,layer |
| R5763 |
T12235 |
T12234 |
punct |
),b |
| R5764 |
T12236 |
T12204 |
punct |
.,consists |
| R5765 |
T12238 |
T12239 |
punct |
(,B |
| R5766 |
T12239 |
T12240 |
meta |
B,performed |
| R5767 |
T12241 |
T12239 |
punct |
),B |
| R5768 |
T12242 |
T12240 |
nsubjpass |
Immunostaining,performed |
| R5769 |
T12243 |
T12242 |
prep |
of,Immunostaining |
| R5770 |
T12244 |
T12245 |
det |
the,section |
| R5771 |
T12245 |
T12243 |
pobj |
section,of |
| R5772 |
T12246 |
T12245 |
compound |
paraffin,section |
| R5773 |
T12247 |
T12240 |
auxpass |
was,performed |
| R5774 |
T12248 |
T12249 |
aux |
using,purified |
| R5775 |
T12249 |
T12240 |
advcl |
purified,performed |
| R5776 |
T12250 |
T12251 |
nmod |
mAb,203 |
| R5777 |
T12251 |
T12249 |
dobj |
203,purified |
| R5778 |
T12252 |
T12253 |
punct |
–,217 |
| R5779 |
T12253 |
T12251 |
prep |
217,203 |
| R5780 |
T12254 |
T12251 |
cc |
and,203 |
| R5781 |
T12255 |
T12256 |
npadvmod |
Cy3,conjugated |
| R5782 |
T12256 |
T12258 |
amod |
conjugated,IgG |
| R5783 |
T12257 |
T12256 |
punct |
-,conjugated |
| R5784 |
T12258 |
T12251 |
conj |
IgG,203 |
| R5785 |
T12259 |
T12258 |
amod |
anti-mouse,IgG |
| R5786 |
T12260 |
T12240 |
punct |
.,performed |
| R5787 |
T12262 |
T12263 |
compound |
Annexin,A7 |
| R5788 |
T12263 |
T12264 |
nsubjpass |
A7,expressed |
| R5789 |
T12265 |
T12264 |
auxpass |
is,expressed |
| R5790 |
T12266 |
T12264 |
prep |
in,expressed |
| R5791 |
T12267 |
T12268 |
det |
both,types |
| R5792 |
T12268 |
T12266 |
pobj |
types,in |
| R5793 |
T12269 |
T12268 |
compound |
cell,types |
| R5794 |
T12270 |
T12268 |
prep |
of,types |
| R5795 |
T12271 |
T12272 |
det |
the,cylinder |
| R5796 |
T12272 |
T12270 |
pobj |
cylinder,of |
| R5797 |
T12273 |
T12272 |
compound |
egg,cylinder |
| R5798 |
T12274 |
T12264 |
prep |
with,expressed |
| R5799 |
T12275 |
T12276 |
det |
a,staining |
| R5800 |
T12276 |
T12274 |
pobj |
staining,with |
| R5801 |
T12277 |
T12276 |
amod |
strong,staining |
| R5802 |
T12278 |
T12276 |
prep |
of,staining |
| R5803 |
T12279 |
T12280 |
det |
the,endoderm |
| R5804 |
T12280 |
T12278 |
pobj |
endoderm,of |
| R5805 |
T12281 |
T12276 |
cc |
and,staining |
| R5806 |
T12282 |
T12283 |
det |
a,staining |
| R5807 |
T12283 |
T12276 |
conj |
staining,staining |
| R5808 |
T12284 |
T12283 |
amod |
weaker,staining |
| R5809 |
T12285 |
T12283 |
prep |
of,staining |
| R5810 |
T12286 |
T12287 |
det |
the,ectoderm |
| R5811 |
T12287 |
T12285 |
pobj |
ectoderm,of |
| R5812 |
T12288 |
T12264 |
punct |
.,expressed |
| R5813 |
T12290 |
T12291 |
det |
The,nuclei |
| R5814 |
T12291 |
T12292 |
nsubj |
nuclei,are |
| R5815 |
T12293 |
T12292 |
acomp |
devoid,are |
| R5816 |
T12294 |
T12293 |
prep |
of,devoid |
| R5817 |
T12295 |
T12296 |
amod |
immune,reactions |
| R5818 |
T12296 |
T12294 |
pobj |
reactions,of |
| R5819 |
T12297 |
T12292 |
punct |
.,are |
| R5820 |
T12299 |
T12300 |
punct |
(,control |
| R5821 |
T12301 |
T12300 |
meta |
C,control |
| R5822 |
T12302 |
T12300 |
punct |
),control |
| R5823 |
T12303 |
T12300 |
amod |
Negative,control |
| R5824 |
T12304 |
T12300 |
acl |
using,control |
| R5825 |
T12305 |
T12306 |
det |
the,antibody |
| R5826 |
T12306 |
T12304 |
dobj |
antibody,using |
| R5827 |
T12307 |
T12306 |
amod |
secondary,antibody |
| R5828 |
T12308 |
T12306 |
compound |
Cy3,antibody |
| R5829 |
T12309 |
T12306 |
punct |
-,antibody |
| R5830 |
T12310 |
T12306 |
advmod |
only,antibody |
| R5831 |
T12311 |
T12300 |
punct |
.,control |
| R5832 |
T12313 |
T12314 |
punct |
(,D |
| R5833 |
T12314 |
T12315 |
meta |
D,section |
| R5834 |
T12316 |
T12314 |
punct |
-,D |
| R5835 |
T12317 |
T12314 |
dep |
F,D |
| R5836 |
T12318 |
T12314 |
punct |
),D |
| R5837 |
T12319 |
T12320 |
compound |
Annexin,A7 |
| R5838 |
T12320 |
T12321 |
compound |
A7,expression |
| R5839 |
T12321 |
T12315 |
dep |
expression,section |
| R5840 |
T12322 |
T12321 |
prep |
in,expression |
| R5841 |
T12323 |
T12324 |
det |
the,tube |
| R5842 |
T12324 |
T12322 |
pobj |
tube,in |
| R5843 |
T12325 |
T12324 |
amod |
proximal,tube |
| R5844 |
T12326 |
T12324 |
amod |
neural,tube |
| R5845 |
T12327 |
T12328 |
punct |
(,D |
| R5846 |
T12328 |
T12324 |
parataxis |
D,tube |
| R5847 |
T12329 |
T12328 |
punct |
),D |
| R5848 |
T12330 |
T12324 |
cc |
and,tube |
| R5849 |
T12331 |
T12332 |
amod |
nearby,fold |
| R5850 |
T12332 |
T12324 |
conj |
fold,tube |
| R5851 |
T12333 |
T12332 |
amod |
neural,fold |
| R5852 |
T12334 |
T12335 |
punct |
(,F |
| R5853 |
T12335 |
T12332 |
parataxis |
F,fold |
| R5854 |
T12336 |
T12335 |
nmod |
E,F |
| R5855 |
T12337 |
T12335 |
punct |
",",F |
| R5856 |
T12338 |
T12335 |
punct |
),F |
| R5857 |
T12339 |
T12315 |
punct |
", ",section |
| R5858 |
T12340 |
T12341 |
compound |
embryo,E8 |
| R5859 |
T12341 |
T12315 |
dep |
E8,section |
| R5860 |
T12342 |
T12315 |
punct |
", ",section |
| R5861 |
T12343 |
T12315 |
amod |
transverse,section |
| R5862 |
T12344 |
T12315 |
punct |
.,section |
| R5863 |
T12346 |
T12347 |
nsubjpass |
Immunolabeling,performed |
| R5864 |
T12348 |
T12346 |
prep |
of,Immunolabeling |
| R5865 |
T12349 |
T12350 |
compound |
Annexin,A7 |
| R5866 |
T12350 |
T12348 |
pobj |
A7,of |
| R5867 |
T12351 |
T12347 |
auxpass |
was,performed |
| R5868 |
T12352 |
T12347 |
prep |
with,performed |
| R5869 |
T12353 |
T12354 |
amod |
purified,203 |
| R5870 |
T12354 |
T12352 |
pobj |
203,with |
| R5871 |
T12355 |
T12354 |
nmod |
mAb,203 |
| R5872 |
T12356 |
T12357 |
punct |
–,217 |
| R5873 |
T12357 |
T12354 |
prep |
217,203 |
| R5874 |
T12358 |
T12347 |
cc |
and,performed |
| R5875 |
T12359 |
T12360 |
nsubj |
visualization,was |
| R5876 |
T12360 |
T12347 |
conj |
was,performed |
| R5877 |
T12361 |
T12360 |
prep |
with,was |
| R5878 |
T12362 |
T12363 |
det |
an,IgG |
| R5879 |
T12363 |
T12361 |
pobj |
IgG,with |
| R5880 |
T12364 |
T12365 |
compound |
Alexa,Fluor |
| R5881 |
T12365 |
T12366 |
npadvmod |
Fluor,conjugated |
| R5882 |
T12366 |
T12363 |
amod |
conjugated,IgG |
| R5883 |
T12367 |
T12365 |
nummod |
488,Fluor |
| R5884 |
T12368 |
T12366 |
punct |
-,conjugated |
| R5885 |
T12369 |
T12363 |
amod |
anti-mouse,IgG |
| R5886 |
T12370 |
T12360 |
punct |
.,was |
| R5887 |
T12372 |
T12373 |
punct |
(,D |
| R5888 |
T12373 |
T12374 |
meta |
D,is |
| R5889 |
T12375 |
T12373 |
punct |
),D |
| R5890 |
T12376 |
T12377 |
det |
An,immunostaining |
| R5891 |
T12377 |
T12374 |
nsubj |
immunostaining,is |
| R5892 |
T12378 |
T12377 |
amod |
intense,immunostaining |
| R5893 |
T12379 |
T12380 |
compound |
Annexin,A7 |
| R5894 |
T12380 |
T12377 |
compound |
A7,immunostaining |
| R5895 |
T12381 |
T12374 |
acomp |
detectable,is |
| R5896 |
T12382 |
T12374 |
prep |
in,is |
| R5897 |
T12383 |
T12384 |
det |
the,neuroepithelium |
| R5898 |
T12384 |
T12382 |
pobj |
neuroepithelium,in |
| R5899 |
T12385 |
T12384 |
prep |
of,neuroepithelium |
| R5900 |
T12386 |
T12387 |
det |
the,tube |
| R5901 |
T12387 |
T12385 |
pobj |
tube,of |
| R5902 |
T12388 |
T12387 |
amod |
neural,tube |
| R5903 |
T12389 |
T12390 |
punct |
(,lumen |
| R5904 |
T12390 |
T12374 |
parataxis |
lumen,is |
| R5905 |
T12391 |
T12390 |
dep |
a,lumen |
| R5906 |
T12392 |
T12390 |
punct |
", ",lumen |
| R5907 |
T12393 |
T12390 |
prep |
of,lumen |
| R5908 |
T12394 |
T12395 |
amod |
neural,tube |
| R5909 |
T12395 |
T12393 |
pobj |
tube,of |
| R5910 |
T12396 |
T12390 |
punct |
),lumen |
| R5911 |
T12397 |
T12374 |
punct |
.,is |
| R5912 |
T12399 |
T12400 |
punct |
(,E |
| R5913 |
T12400 |
T12401 |
meta |
E,show |
| R5914 |
T12402 |
T12400 |
punct |
",",E |
| R5915 |
T12403 |
T12400 |
dep |
F,E |
| R5916 |
T12404 |
T12400 |
punct |
),E |
| R5917 |
T12405 |
T12406 |
amod |
Higher,magnifications |
| R5918 |
T12406 |
T12401 |
nsubj |
magnifications,show |
| R5919 |
T12407 |
T12406 |
prep |
of,magnifications |
| R5920 |
T12408 |
T12409 |
det |
the,neuroepithelium |
| R5921 |
T12409 |
T12407 |
pobj |
neuroepithelium,of |
| R5922 |
T12410 |
T12411 |
mark |
that,expressed |
| R5923 |
T12411 |
T12401 |
ccomp |
expressed,show |
| R5924 |
T12412 |
T12413 |
compound |
Annexin,A7 |
| R5925 |
T12413 |
T12411 |
nsubjpass |
A7,expressed |
| R5926 |
T12414 |
T12411 |
auxpass |
is,expressed |
| R5927 |
T12415 |
T12411 |
prep |
in,expressed |
| R5928 |
T12416 |
T12417 |
det |
the,cytosol |
| R5929 |
T12417 |
T12415 |
pobj |
cytosol,in |
| R5930 |
T12418 |
T12401 |
punct |
.,show |
| R5931 |
T12420 |
T12421 |
nsubj |
Arrowheads,point |
| R5932 |
T12422 |
T12421 |
prep |
to,point |
| R5933 |
T12423 |
T12424 |
compound |
Annexin,A7 |
| R5934 |
T12424 |
T12425 |
compound |
A7,immunoreactivity |
| R5935 |
T12425 |
T12422 |
pobj |
immunoreactivity,to |
| R5936 |
T12426 |
T12421 |
prep |
in,point |
| R5937 |
T12427 |
T12428 |
det |
the,cytosol |
| R5938 |
T12428 |
T12426 |
pobj |
cytosol,in |
| R5939 |
T12429 |
T12421 |
punct |
.,point |
| R5940 |
T12431 |
T12432 |
punct |
(,tube |
| R5941 |
T12433 |
T12432 |
meta |
G,tube |
| R5942 |
T12434 |
T12432 |
punct |
),tube |
| R5943 |
T12435 |
T12436 |
compound |
Phase,contrast |
| R5944 |
T12436 |
T12432 |
dep |
contrast,tube |
| R5945 |
T12437 |
T12432 |
punct |
", ",tube |
| R5946 |
T12438 |
T12439 |
compound |
embryo,E13 |
| R5947 |
T12439 |
T12432 |
dep |
E13,tube |
| R5948 |
T12440 |
T12432 |
punct |
", ",tube |
| R5949 |
T12441 |
T12432 |
amod |
caudal,tube |
| R5950 |
T12442 |
T12432 |
amod |
neural,tube |
| R5951 |
T12443 |
T12432 |
punct |
.,tube |
| R5952 |
T12445 |
T12446 |
punct |
(,H |
| R5953 |
T12446 |
T12447 |
meta |
H,performed |
| R5954 |
T12448 |
T12446 |
punct |
),H |
| R5955 |
T12449 |
T12447 |
nsubjpass |
Immunostaining,performed |
| R5956 |
T12450 |
T12449 |
prep |
of,Immunostaining |
| R5957 |
T12451 |
T12452 |
det |
the,section |
| R5958 |
T12452 |
T12450 |
pobj |
section,of |
| R5959 |
T12453 |
T12452 |
compound |
paraffin,section |
| R5960 |
T12454 |
T12447 |
auxpass |
was,performed |
| R5961 |
T12455 |
T12447 |
advcl |
using,performed |
| R5962 |
T12456 |
T12457 |
amod |
purified,203 |
| R5963 |
T12457 |
T12455 |
dobj |
203,using |
| R5964 |
T12458 |
T12457 |
nmod |
mAb,203 |
| R5965 |
T12459 |
T12460 |
punct |
–,217 |
| R5966 |
T12460 |
T12457 |
prep |
217,203 |
| R5967 |
T12461 |
T12457 |
cc |
and,203 |
| R5968 |
T12462 |
T12463 |
compound |
Alexa,Fluor |
| R5969 |
T12463 |
T12464 |
npadvmod |
Fluor,conjugated |
| R5970 |
T12464 |
T12467 |
amod |
conjugated,IgG |
| R5971 |
T12465 |
T12463 |
nummod |
488,Fluor |
| R5972 |
T12466 |
T12464 |
punct |
-,conjugated |
| R5973 |
T12467 |
T12457 |
conj |
IgG,203 |
| R5974 |
T12468 |
T12467 |
amod |
anti-mouse,IgG |
| R5975 |
T12469 |
T12447 |
punct |
.,performed |
| R5976 |
T12471 |
T12472 |
punct |
(,I |
| R5977 |
T12472 |
T12473 |
meta |
I,control |
| R5978 |
T12474 |
T12472 |
punct |
),I |
| R5979 |
T12475 |
T12473 |
amod |
Negative,control |
| R5980 |
T12476 |
T12473 |
acl |
using,control |
| R5981 |
T12477 |
T12478 |
det |
the,antibody |
| R5982 |
T12478 |
T12476 |
dobj |
antibody,using |
| R5983 |
T12479 |
T12478 |
amod |
secondary,antibody |
| R5984 |
T12480 |
T12481 |
nmod |
Alexa,Fluor |
| R5985 |
T12481 |
T12478 |
nmod |
Fluor,antibody |
| R5986 |
T12482 |
T12481 |
nummod |
488,Fluor |
| R5987 |
T12483 |
T12478 |
punct |
-,antibody |
| R5988 |
T12484 |
T12478 |
advmod |
only,antibody |
| R5989 |
T12485 |
T12473 |
punct |
.,control |
| R5990 |
T12488 |
T12487 |
punct |
", ",Bar |
| R5991 |
T12489 |
T12490 |
nummod |
20,μm |
| R5992 |
T12490 |
T12487 |
appos |
μm,Bar |
| R5993 |
T12491 |
T12487 |
punct |
.,Bar |
| R771 |
T2289 |
T2290 |
compound |
Annexin,A7 |
| R772 |
T2290 |
T2291 |
nsubjpass |
A7,expressed |
| R773 |
T2292 |
T2291 |
auxpass |
is,expressed |
| R774 |
T2293 |
T2291 |
prep |
in,expressed |
| R775 |
T2294 |
T2295 |
det |
the,embryo |
| R776 |
T2295 |
T2293 |
pobj |
embryo,in |
| R777 |
T2296 |
T2295 |
amod |
early,embryo |
| R778 |
T2297 |
T2295 |
compound |
mouse,embryo |
| R779 |
T2299 |
T2300 |
advmod |
First,examined |
| R780 |
T2301 |
T2300 |
nsubj |
we,examined |
| R781 |
T2302 |
T2303 |
det |
the,expression |
| R782 |
T2303 |
T2300 |
dobj |
expression,examined |
| R783 |
T2304 |
T2303 |
prep |
of,expression |
| R784 |
T2305 |
T2306 |
compound |
Annexin,A7 |
| R785 |
T2306 |
T2304 |
pobj |
A7,of |
| R786 |
T2307 |
T2303 |
prep |
in,expression |
| R787 |
T2308 |
T2309 |
compound |
ES,cells |
| R788 |
T2309 |
T2307 |
pobj |
cells,in |
| R789 |
T2310 |
T2311 |
punct |
(,Bruce4 |
| R790 |
T2311 |
T2309 |
parataxis |
Bruce4,cells |
| R791 |
T2312 |
T2311 |
punct |
", ",Bruce4 |
| R792 |
T2313 |
T2309 |
acl |
established,cells |
| R793 |
T2314 |
T2313 |
prep |
from,established |
| R794 |
T2315 |
T2316 |
compound |
C57BL,6J |
| R795 |
T2316 |
T2318 |
compound |
6J,mice |
| R796 |
T2317 |
T2316 |
punct |
/,6J |
| R797 |
T2318 |
T2314 |
pobj |
mice,from |
| R798 |
T2319 |
T2309 |
punct |
),cells |
| R799 |
T2320 |
T2309 |
cc |
and,cells |
| R800 |
T2321 |
T2322 |
det |
the,stages |
| R801 |
T2322 |
T2309 |
conj |
stages,cells |
| R802 |
T2323 |
T2322 |
amod |
early,stages |
| R803 |
T2324 |
T2322 |
prep |
of,stages |
| R804 |
T2325 |
T2326 |
nmod |
mouse,development |
| R805 |
T2326 |
T2324 |
pobj |
development,of |
| R806 |
T2327 |
T2326 |
amod |
embryonic,development |
| R807 |
T2328 |
T2300 |
prep |
at,examined |
| R808 |
T2329 |
T2330 |
det |
the,level |
| R809 |
T2330 |
T2328 |
pobj |
level,at |
| R810 |
T2331 |
T2330 |
compound |
mRNA,level |
| R811 |
T2332 |
T2300 |
prep |
by,examined |
| R812 |
T2333 |
T2334 |
compound |
northern,blot |
| R813 |
T2334 |
T2335 |
compound |
blot,analysis |
| R814 |
T2335 |
T2332 |
pobj |
analysis,by |
| R815 |
T2336 |
T2300 |
cc |
and,examined |
| R816 |
T2337 |
T2300 |
conj |
at,examined |
| R817 |
T2338 |
T2339 |
det |
the,level |
| R818 |
T2339 |
T2337 |
pobj |
level,at |
| R819 |
T2340 |
T2339 |
compound |
protein,level |
| R820 |
T2341 |
T2337 |
prep |
by,at |
| R821 |
T2342 |
T2343 |
compound |
Western,blotting |
| R822 |
T2343 |
T2341 |
pobj |
blotting,by |
| R823 |
T2344 |
T2343 |
cc |
and,blotting |
| R824 |
T2345 |
T2343 |
conj |
immunohistochemistry,blotting |
| R825 |
T2346 |
T2337 |
punct |
", ",at |
| R826 |
T2347 |
T2337 |
conj |
respectively,at |
| R827 |
T2348 |
T2300 |
punct |
.,examined |
| R828 |
T2350 |
T2351 |
compound |
Northern,blot |
| R829 |
T2351 |
T2352 |
compound |
blot,analysis |
| R830 |
T2352 |
T2353 |
nsubj |
analysis,shows |
| R831 |
T2354 |
T2353 |
prep |
in,shows |
| R832 |
T2355 |
T2356 |
compound |
ES,cells |
| R833 |
T2356 |
T2354 |
pobj |
cells,in |
| R834 |
T2357 |
T2356 |
punct |
-,cells |
| R835 |
T2358 |
T2354 |
cc |
and,in |
| R836 |
T2359 |
T2354 |
conj |
at,in |
| R837 |
T2360 |
T2361 |
amod |
embryonic,E7 |
| R838 |
T2361 |
T2359 |
pobj |
E7,at |
| R839 |
T2362 |
T2361 |
compound |
days,E7 |
| R840 |
T2363 |
T2361 |
punct |
", ",E7 |
| R841 |
T2364 |
T2361 |
conj |
E11,E7 |
| R842 |
T2365 |
T2364 |
punct |
", ",E11 |
| R843 |
T2366 |
T2364 |
conj |
E15,E11 |
| R844 |
T2367 |
T2366 |
punct |
", ",E15 |
| R845 |
T2368 |
T2366 |
cc |
and,E15 |
| R846 |
T2369 |
T2366 |
conj |
E17,E15 |
| R847 |
T2370 |
T2371 |
nummod |
two,species |
| R848 |
T2371 |
T2353 |
dobj |
species,shows |
| R849 |
T2372 |
T2371 |
compound |
mRNA,species |
| R850 |
T2373 |
T2371 |
prep |
of,species |
| R851 |
T2374 |
T2375 |
nummod |
1.8,kb |
| R852 |
T2375 |
T2373 |
pobj |
kb,of |
| R853 |
T2376 |
T2375 |
cc |
and,kb |
| R854 |
T2377 |
T2378 |
nummod |
2.4,kb |
| R855 |
T2378 |
T2375 |
conj |
kb,kb |
| R856 |
T2379 |
T2371 |
punct |
", ",species |
| R857 |
T2380 |
T2381 |
dep |
which,result |
| R858 |
T2381 |
T2371 |
relcl |
result,species |
| R859 |
T2382 |
T2381 |
prep |
from,result |
| R860 |
T2383 |
T2384 |
amod |
alternative,splicing |
| R861 |
T2384 |
T2382 |
pobj |
splicing,from |
| R862 |
T2385 |
T2381 |
prep |
in,result |
| R863 |
T2386 |
T2387 |
det |
the,end |
| R864 |
T2387 |
T2385 |
pobj |
end,in |
| R865 |
T2388 |
T2387 |
amod |
untranslated,end |
| R866 |
T2389 |
T2387 |
nummod |
3,end |
| R867 |
T2390 |
T2389 |
punct |
',3 |
| R868 |
T2391 |
T2392 |
punct |
(,Fig. |
| R869 |
T2392 |
T2353 |
parataxis |
Fig.,shows |
| R870 |
T2393 |
T2392 |
nummod |
1A,Fig. |
| R871 |
T2394 |
T2392 |
punct |
),Fig. |
| R872 |
T2395 |
T2396 |
punct |
[,11 |
| R873 |
T2396 |
T2353 |
parataxis |
11,shows |
| R874 |
T2397 |
T2396 |
punct |
],11 |
| R875 |
T2398 |
T2353 |
punct |
.,shows |
| R876 |
T2400 |
T2401 |
nsubj |
We,found |
| R877 |
T2402 |
T2403 |
amod |
similar,levels |
| R878 |
T2403 |
T2401 |
dobj |
levels,found |
| R879 |
T2404 |
T2405 |
compound |
Annexin,A7 |
| R880 |
T2405 |
T2403 |
compound |
A7,levels |
| R881 |
T2406 |
T2403 |
compound |
mRNA,levels |
| R882 |
T2407 |
T2401 |
prep |
in,found |
| R883 |
T2408 |
T2409 |
det |
the,stages |
| R884 |
T2409 |
T2407 |
pobj |
stages,in |
| R885 |
T2410 |
T2409 |
nummod |
four,stages |
| R886 |
T2411 |
T2409 |
amod |
embryonic,stages |
| R887 |
T2412 |
T2401 |
punct |
.,found |
| R888 |
T2414 |
T2415 |
nsubj |
Reprobing,verified |
| R889 |
T2415 |
T2422 |
ccomp |
verified,is |
| R890 |
T2416 |
T2414 |
prep |
with,Reprobing |
| R891 |
T2417 |
T2418 |
det |
a,probe |
| R892 |
T2418 |
T2416 |
pobj |
probe,with |
| R893 |
T2419 |
T2420 |
compound |
β,actin |
| R894 |
T2420 |
T2418 |
compound |
actin,probe |
| R895 |
T2421 |
T2420 |
punct |
-,actin |
| R896 |
T2423 |
T2424 |
amod |
equal,loading |
| R897 |
T2424 |
T2415 |
dobj |
loading,verified |
| R898 |
T2425 |
T2422 |
punct |
;,is |
| R899 |
T2426 |
T2427 |
det |
the,appearance |
| R900 |
T2427 |
T2422 |
nsubj |
appearance,is |
| R901 |
T2428 |
T2427 |
prep |
of,appearance |
| R902 |
T2429 |
T2430 |
det |
a,species |
| R903 |
T2430 |
T2428 |
pobj |
species,of |
| R904 |
T2431 |
T2432 |
amod |
faster,migrating |
| R905 |
T2432 |
T2430 |
amod |
migrating,species |
| R906 |
T2433 |
T2430 |
compound |
mRNA,species |
| R907 |
T2434 |
T2430 |
prep |
in,species |
| R908 |
T2435 |
T2434 |
pobj |
addition,in |
| R909 |
T2436 |
T2435 |
prep |
to,addition |
| R910 |
T2437 |
T2438 |
det |
the,species |
| R911 |
T2438 |
T2436 |
pobj |
species,to |
| R912 |
T2439 |
T2440 |
nummod |
2.0,kb |
| R913 |
T2440 |
T2438 |
compound |
kb,species |
| R914 |
T2441 |
T2422 |
acomp |
characteristic,is |
| R915 |
T2442 |
T2422 |
prep |
for,is |
| R916 |
T2443 |
T2444 |
compound |
β,actin |
| R917 |
T2444 |
T2442 |
pobj |
actin,for |
| R918 |
T2445 |
T2444 |
punct |
-,actin |
| R919 |
T2446 |
T2447 |
punct |
[,12 |
| R920 |
T2447 |
T2422 |
parataxis |
12,is |
| R921 |
T2448 |
T2447 |
punct |
],12 |
| R922 |
T2449 |
T2422 |
punct |
.,is |
| R923 |
T2451 |
T2452 |
prep |
On,express |
| R924 |
T2453 |
T2454 |
det |
the,level |
| R925 |
T2454 |
T2451 |
pobj |
level,On |
| R926 |
T2455 |
T2454 |
compound |
protein,level |
| R927 |
T2456 |
T2452 |
punct |
", ",express |
| R928 |
T2457 |
T2458 |
compound |
ES,cells |
| R929 |
T2458 |
T2452 |
nsubj |
cells,express |
| R930 |
T2459 |
T2460 |
advmod |
only,isoform |
| R931 |
T2460 |
T2452 |
dobj |
isoform,express |
| R932 |
T2461 |
T2460 |
det |
the,isoform |
| R933 |
T2462 |
T2460 |
amod |
smaller,isoform |
| R934 |
T2463 |
T2464 |
compound |
Annexin,A7 |
| R935 |
T2464 |
T2460 |
compound |
A7,isoform |
| R936 |
T2465 |
T2460 |
prep |
of,isoform |
| R937 |
T2466 |
T2467 |
nummod |
47,kDa |
| R938 |
T2467 |
T2465 |
pobj |
kDa,of |
| R939 |
T2468 |
T2469 |
punct |
(,1B |
| R940 |
T2469 |
T2452 |
parataxis |
1B,express |
| R941 |
T2470 |
T2469 |
compound |
Fig.,1B |
| R942 |
T2471 |
T2469 |
punct |
", ",1B |
| R943 |
T2472 |
T2473 |
nmod |
neuro,cells |
| R944 |
T2473 |
T2476 |
nsubjpass |
cells,included |
| R945 |
T2474 |
T2472 |
punct |
-,neuro |
| R946 |
T2475 |
T2472 |
nummod |
2a,neuro |
| R947 |
T2476 |
T2469 |
ccomp |
included,1B |
| R948 |
T2477 |
T2473 |
cc |
and,cells |
| R949 |
T2478 |
T2479 |
compound |
heart,tissue |
| R950 |
T2479 |
T2473 |
conj |
tissue,cells |
| R951 |
T2480 |
T2476 |
auxpass |
are,included |
| R952 |
T2481 |
T2476 |
prep |
for,included |
| R953 |
T2482 |
T2481 |
pobj |
control,for |
| R954 |
T2483 |
T2469 |
punct |
),1B |
| R955 |
T2484 |
T2452 |
punct |
.,express |
| R956 |
T2486 |
T2487 |
nsubj |
Immunohistochemistry,confirmed |
| R957 |
T2488 |
T2489 |
det |
the,presence |
| R958 |
T2489 |
T2487 |
dobj |
presence,confirmed |
| R959 |
T2490 |
T2489 |
prep |
of,presence |
| R960 |
T2491 |
T2492 |
det |
the,protein |
| R961 |
T2492 |
T2490 |
pobj |
protein,of |
| R962 |
T2493 |
T2489 |
prep |
during,presence |
| R963 |
T2494 |
T2495 |
amod |
early,development |
| R964 |
T2495 |
T2493 |
pobj |
development,during |
| R965 |
T2496 |
T2487 |
punct |
.,confirmed |
| R966 |
T2498 |
T2499 |
compound |
Annexin,A7 |
| R967 |
T2499 |
T2500 |
compound |
A7,immunoreactivity |
| R968 |
T2500 |
T2501 |
nsubjpass |
immunoreactivity,observed |
| R969 |
T2502 |
T2500 |
acl |
using,immunoreactivity |
| R970 |
T2503 |
T2504 |
nmod |
mAb,203 |
| R971 |
T2504 |
T2502 |
dobj |
203,using |
| R972 |
T2505 |
T2506 |
punct |
–,217 |
| R973 |
T2506 |
T2504 |
prep |
217,203 |
| R974 |
T2507 |
T2504 |
punct |
", ",203 |
| R975 |
T2508 |
T2509 |
dep |
which,recognizes |
| R976 |
T2509 |
T2504 |
relcl |
recognizes,203 |
| R977 |
T2510 |
T2509 |
advmod |
specifically,recognizes |
| R978 |
T2511 |
T2512 |
det |
both,isoforms |
| R979 |
T2512 |
T2509 |
dobj |
isoforms,recognizes |
| R980 |
T2513 |
T2512 |
prep |
of,isoforms |
| R981 |
T2514 |
T2515 |
compound |
Annexin,A7 |
| R982 |
T2515 |
T2513 |
pobj |
A7,of |
| R983 |
T2516 |
T2501 |
punct |
", ",observed |
| R984 |
T2517 |
T2501 |
aux |
can,observed |
| R985 |
T2518 |
T2501 |
auxpass |
be,observed |
| R986 |
T2519 |
T2501 |
prep |
in,observed |
| R987 |
T2520 |
T2521 |
det |
the,types |
| R988 |
T2521 |
T2519 |
pobj |
types,in |
| R989 |
T2522 |
T2521 |
nummod |
two,types |
| R990 |
T2523 |
T2521 |
compound |
cell,types |
| R991 |
T2524 |
T2521 |
prep |
of,types |
| R992 |
T2525 |
T2526 |
det |
the,stage |
| R993 |
T2526 |
T2524 |
pobj |
stage,of |
| R994 |
T2527 |
T2526 |
compound |
cylinder,stage |
| R995 |
T2528 |
T2501 |
prep |
at,observed |
| R996 |
T2529 |
T2528 |
pobj |
E5,at |
| R997 |
T2530 |
T2501 |
prep |
with,observed |
| R998 |
T2531 |
T2532 |
det |
a,expression |
| R999 |
T2532 |
T2530 |
pobj |
expression,with |
