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PMC:1064854 / 6275-8446 JSONTXT 6 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T5691 0-1 DT denotes A
T5693 2-10 NN denotes neomycin
T5695 10-11 HYPH denotes -
T5694 11-16 VBN denotes based
T5696 17-21 NN denotes gene
T5697 22-31 NN denotes targeting
T5692 32-38 NN denotes vector
T5699 39-42 VBD denotes was
T5698 43-52 VBN denotes generated
T5700 53-55 TO denotes to
T5701 56-62 VB denotes delete
T5702 63-68 NNS denotes exons
T5703 69-70 CD denotes 4
T5704 71-74 CC denotes and
T5705 75-76 CD denotes 5
T5706 77-79 IN denotes of
T5707 80-83 DT denotes the
T5709 84-90 JJ denotes murine
T5710 91-94 NN denotes PGC
T5712 94-95 HYPH denotes -
T5711 95-97 NN denotes 1α
T5708 98-102 NN denotes gene
T5713 102-103 . denotes .
T5714 103-227 sentence denotes The targeting event resulted in a 3′ homologous recombination with insertion of the remainder of the construct (Figure 1A).
T5715 104-107 DT denotes The
T5717 108-117 NN denotes targeting
T5716 118-123 NN denotes event
T5718 124-132 VBD denotes resulted
T5719 133-135 IN denotes in
T5720 136-137 DT denotes a
T5722 138-139 CD denotes 3
T5723 139-140 SYM denotes ′
T5724 141-151 JJ denotes homologous
T5721 152-165 NN denotes recombination
T5725 166-170 IN denotes with
T5726 171-180 NN denotes insertion
T5727 181-183 IN denotes of
T5728 184-187 DT denotes the
T5729 188-197 NN denotes remainder
T5730 198-200 IN denotes of
T5731 201-204 DT denotes the
T5732 205-214 NN denotes construct
T5733 215-216 -LRB- denotes (
T5735 216-222 NN denotes Figure
T5734 223-225 NN denotes 1A
T5736 225-226 -RRB- denotes )
T5737 226-227 . denotes .
T5738 227-316 sentence denotes The insertion/recombination event was confirmed by Southern blotting and DNA sequencing.
T5739 228-231 DT denotes The
T5741 232-241 NN denotes insertion
T5743 241-242 HYPH denotes /
T5742 242-255 NN denotes recombination
T5740 256-261 NN denotes event
T5745 262-265 VBD denotes was
T5744 266-275 VBN denotes confirmed
T5746 276-278 IN denotes by
T5747 279-287 NNP denotes Southern
T5748 288-296 NN denotes blotting
T5749 297-300 CC denotes and
T5750 301-304 NN denotes DNA
T5751 305-315 NN denotes sequencing
T5752 315-316 . denotes .
T5753 316-477 sentence denotes The insertion caused an exon 3 duplication between exons 5 and 6 that creates a coding region frameshift resulting in a premature termination at amino acid 255.
T5754 317-320 DT denotes The
T5755 321-330 NN denotes insertion
T5756 331-337 VBD denotes caused
T5757 338-340 DT denotes an
T5759 341-345 NN denotes exon
T5760 346-347 CD denotes 3
T5758 348-359 NN denotes duplication
T5761 360-367 IN denotes between
T5762 368-373 NNS denotes exons
T5763 374-375 CD denotes 5
T5764 376-379 CC denotes and
T5765 380-381 CD denotes 6
T5766 382-386 WDT denotes that
T5767 387-394 VBZ denotes creates
T5768 395-396 DT denotes a
T5770 397-403 VBG denotes coding
T5771 404-410 NN denotes region
T5769 411-421 NN denotes frameshift
T5772 422-431 VBG denotes resulting
T5773 432-434 IN denotes in
T5774 435-436 DT denotes a
T5776 437-446 JJ denotes premature
T5775 447-458 NN denotes termination
T5777 459-461 IN denotes at
T5778 462-467 NN denotes amino
T5779 468-472 NN denotes acid
T5780 473-476 CD denotes 255
T5781 476-477 . denotes .
T5782 477-598 sentence denotes Germline transmission of the mutant allele was confirmed using Southern blotting (Figure 1B) and PCR (unpublished data).
T5783 478-486 NN denotes Germline
T5784 487-499 NN denotes transmission
T5786 500-502 IN denotes of
T5787 503-506 DT denotes the
T5789 507-513 NN denotes mutant
T5788 514-520 NN denotes allele
T5790 521-524 VBD denotes was
T5785 525-534 VBN denotes confirmed
T5791 535-540 VBG denotes using
T5792 541-549 NNP denotes Southern
T5793 550-558 NN denotes blotting
T5794 559-560 -LRB- denotes (
T5796 560-566 NN denotes Figure
T5795 567-569 NN denotes 1B
T5797 569-570 -RRB- denotes )
T5798 571-574 CC denotes and
T5799 575-578 NN denotes PCR
T5800 579-580 -LRB- denotes (
T5802 580-591 JJ denotes unpublished
T5801 592-596 NNS denotes data
T5803 596-597 -RRB- denotes )
T5804 597-598 . denotes .
T5805 598-787 sentence denotes The PGC-1α gene disruption resulted in an unstable transcript that could not be detected by RNA blot analysis in heart and other tissues in PGC-1α−/− mice (Figure 1C and unpublished data).
T5806 599-602 DT denotes The
T5808 603-606 NN denotes PGC
T5810 606-607 HYPH denotes -
T5809 607-609 NN denotes 1α
T5811 610-614 NN denotes gene
T5807 615-625 NN denotes disruption
T5812 626-634 VBD denotes resulted
T5813 635-637 IN denotes in
T5814 638-640 DT denotes an
T5816 641-649 JJ denotes unstable
T5815 650-660 NN denotes transcript
T5817 661-665 WDT denotes that
T5819 666-671 MD denotes could
T5820 672-675 RB denotes not
T5821 676-678 VB denotes be
T5818 679-687 VBN denotes detected
T5822 688-690 IN denotes by
T5823 691-694 NN denotes RNA
T5825 695-699 NN denotes blot
T5824 700-708 NN denotes analysis
T5826 709-711 IN denotes in
T5827 712-717 NN denotes heart
T5828 718-721 CC denotes and
T5829 722-727 JJ denotes other
T5830 728-735 NNS denotes tissues
T5831 736-738 IN denotes in
T5832 739-742 NN denotes PGC
T5834 742-743 HYPH denotes -
T5833 743-745 NN denotes 1α
T5836 745-746 SYM denotes −
T5837 746-747 HYPH denotes /
T5838 747-748 SYM denotes −
T5835 749-753 NNS denotes mice
T5839 754-755 -LRB- denotes (
T5841 755-761 NN denotes Figure
T5840 762-764 NN denotes 1C
T5842 765-768 CC denotes and
T5843 769-780 JJ denotes unpublished
T5844 781-785 NNS denotes data
T5845 785-786 -RRB- denotes )
T5846 786-787 . denotes .
T5847 787-876 sentence denotes Quantitative RT-PCR was utilized to further evaluate the efficacy of the gene targeting.
T5848 788-800 JJ denotes Quantitative
T5850 801-803 NN denotes RT
T5851 803-804 HYPH denotes -
T5849 804-807 NN denotes PCR
T5853 808-811 VBD denotes was
T5852 812-820 VBN denotes utilized
T5854 821-823 TO denotes to
T5856 824-831 RB denotes further
T5855 832-840 VB denotes evaluate
T5857 841-844 DT denotes the
T5858 845-853 NN denotes efficacy
T5859 854-856 IN denotes of
T5860 857-860 DT denotes the
T5862 861-865 NN denotes gene
T5861 866-875 NN denotes targeting
T5863 875-876 . denotes .
T5864 876-1122 sentence denotes For these studies, PCR primers were designed to amplify a region of the PGC-1α gene transcript containing the exon 5–6 border (predicted to be absent in PGC-1α−/− mice) or the exon 5–3 border (predicted to be present only in the PGC-1α−/− mice).
T5865 877-880 IN denotes For
T5867 881-886 DT denotes these
T5868 887-894 NNS denotes studies
T5869 894-896 , denotes ,
T5870 896-899 NN denotes PCR
T5871 900-907 NNS denotes primers
T5872 908-912 VBD denotes were
T5866 913-921 VBN denotes designed
T5873 922-924 TO denotes to
T5874 925-932 VB denotes amplify
T5875 933-934 DT denotes a
T5876 935-941 NN denotes region
T5877 942-944 IN denotes of
T5878 945-948 DT denotes the
T5880 949-952 NN denotes PGC
T5882 952-953 HYPH denotes -
T5881 953-955 NN denotes 1α
T5883 956-960 NN denotes gene
T5879 961-971 NN denotes transcript
T5884 972-982 VBG denotes containing
T5885 983-986 DT denotes the
T5887 987-991 NN denotes exon
T5888 992-993 CD denotes 5
T5889 993-994 SYM denotes –
T5890 994-995 CD denotes 6
T5886 996-1002 NN denotes border
T5891 1003-1004 -LRB- denotes (
T5892 1004-1013 VBN denotes predicted
T5893 1014-1016 TO denotes to
T5894 1017-1019 VB denotes be
T5895 1020-1026 JJ denotes absent
T5896 1027-1029 IN denotes in
T5897 1030-1033 NN denotes PGC
T5899 1033-1034 HYPH denotes -
T5898 1034-1036 NN denotes 1α
T5901 1036-1037 SYM denotes −
T5902 1037-1038 HYPH denotes /
T5903 1038-1039 SYM denotes −
T5900 1040-1044 NNS denotes mice
T5904 1044-1045 -RRB- denotes )
T5905 1046-1048 CC denotes or
T5906 1049-1052 DT denotes the
T5908 1053-1057 NN denotes exon
T5909 1058-1059 CD denotes 5
T5910 1059-1060 SYM denotes –
T5911 1060-1061 CD denotes 3
T5907 1062-1068 NN denotes border
T5912 1069-1070 -LRB- denotes (
T5913 1070-1079 VBN denotes predicted
T5914 1080-1082 TO denotes to
T5915 1083-1085 VB denotes be
T5916 1086-1093 JJ denotes present
T5917 1094-1098 RB denotes only
T5918 1099-1101 IN denotes in
T5919 1102-1105 DT denotes the
T5921 1106-1109 NN denotes PGC
T5923 1109-1110 HYPH denotes -
T5922 1110-1112 NN denotes 1α
T5924 1112-1113 SYM denotes −
T5925 1113-1114 HYPH denotes /
T5926 1114-1115 SYM denotes −
T5920 1116-1120 NNS denotes mice
T5927 1120-1121 -RRB- denotes )
T5928 1121-1122 . denotes .
T5929 1122-1228 sentence denotes The exon 5–6 amplicon was detected in heart and BAT of wild-type (WT) but not PGC-1α−/− mice (Figure 1D).
T5930 1123-1126 DT denotes The
T5932 1127-1131 NN denotes exon
T5933 1132-1133 CD denotes 5
T5934 1133-1134 SYM denotes –
T5935 1134-1135 CD denotes 6
T5931 1136-1144 NN denotes amplicon
T5937 1145-1148 VBD denotes was
T5936 1149-1157 VBN denotes detected
T5938 1158-1160 IN denotes in
T5939 1161-1166 NN denotes heart
T5940 1167-1170 CC denotes and
T5941 1171-1174 NN denotes BAT
T5942 1175-1177 IN denotes of
T5943 1178-1182 JJ denotes wild
T5945 1182-1183 HYPH denotes -
T5944 1183-1187 NN denotes type
T5947 1188-1189 -LRB- denotes (
T5948 1189-1191 NN denotes WT
T5949 1191-1192 -RRB- denotes )
T5950 1193-1196 CC denotes but
T5951 1197-1200 RB denotes not
T5952 1201-1204 NN denotes PGC
T5954 1204-1205 HYPH denotes -
T5953 1205-1207 NN denotes 1α
T5955 1207-1208 SYM denotes −
T5956 1208-1209 HYPH denotes /
T5957 1209-1210 SYM denotes −
T5946 1211-1215 NNS denotes mice
T5958 1216-1217 -LRB- denotes (
T5960 1217-1223 NN denotes Figure
T5959 1224-1226 NN denotes 1D
T5961 1226-1227 -RRB- denotes )
T5962 1227-1228 . denotes .
T5963 1228-1309 sentence denotes Conversely, the exon 5–3 product was present only in PGC-1α−/− mice (Figure 1D).
T5964 1229-1239 RB denotes Conversely
T5966 1239-1241 , denotes ,
T5967 1241-1244 DT denotes the
T5969 1245-1249 NN denotes exon
T5970 1250-1251 CD denotes 5
T5971 1251-1252 SYM denotes –
T5972 1252-1253 CD denotes 3
T5968 1254-1261 NN denotes product
T5965 1262-1265 VBD denotes was
T5973 1266-1273 JJ denotes present
T5974 1274-1278 RB denotes only
T5975 1279-1281 IN denotes in
T5976 1282-1285 NN denotes PGC
T5978 1285-1286 HYPH denotes -
T5977 1286-1288 NN denotes 1α
T5980 1288-1289 SYM denotes −
T5981 1289-1290 HYPH denotes /
T5982 1290-1291 SYM denotes −
T5979 1292-1296 NNS denotes mice
T5983 1297-1298 -LRB- denotes (
T5985 1298-1304 NN denotes Figure
T5984 1305-1307 NN denotes 1D
T5986 1307-1308 -RRB- denotes )
T5987 1308-1309 . denotes .
T5988 1309-1523 sentence denotes An exon 10–11 border amplicon (predicted to be present in both genotypes) was detected in WT and PGC-1α−/− mice, but was greatly diminished in the PGC-1α−/− mice, indicating that the mutant transcript is unstable.
T5989 1310-1312 DT denotes An
T5991 1313-1317 NN denotes exon
T5992 1318-1320 CD denotes 10
T5993 1320-1321 SYM denotes –
T5994 1321-1323 CD denotes 11
T5995 1324-1330 NN denotes border
T5990 1331-1339 NN denotes amplicon
T5997 1340-1341 -LRB- denotes (
T5998 1341-1350 VBN denotes predicted
T5999 1351-1353 TO denotes to
T6000 1354-1356 VB denotes be
T6001 1357-1364 JJ denotes present
T6002 1365-1367 IN denotes in
T6003 1368-1372 DT denotes both
T6004 1373-1382 NNS denotes genotypes
T6005 1382-1383 -RRB- denotes )
T6006 1384-1387 VBD denotes was
T5996 1388-1396 VBN denotes detected
T6007 1397-1399 IN denotes in
T6008 1400-1402 NN denotes WT
T6010 1403-1406 CC denotes and
T6011 1407-1410 NN denotes PGC
T6013 1410-1411 HYPH denotes -
T6012 1411-1413 NN denotes 1α
T6014 1413-1414 SYM denotes −
T6015 1414-1415 HYPH denotes /
T6016 1415-1416 SYM denotes −
T6009 1417-1421 NNS denotes mice
T6017 1421-1423 , denotes ,
T6018 1423-1426 CC denotes but
T6019 1427-1430 VBD denotes was
T6021 1431-1438 RB denotes greatly
T6020 1439-1449 VBN denotes diminished
T6022 1450-1452 IN denotes in
T6023 1453-1456 DT denotes the
T6025 1457-1460 NN denotes PGC
T6027 1460-1461 HYPH denotes -
T6026 1461-1463 NN denotes 1α
T6028 1463-1464 SYM denotes −
T6029 1464-1465 HYPH denotes /
T6030 1465-1466 SYM denotes −
T6024 1467-1471 NNS denotes mice
T6031 1471-1473 , denotes ,
T6032 1473-1483 VBG denotes indicating
T6033 1484-1488 IN denotes that
T6035 1489-1492 DT denotes the
T6037 1493-1499 NN denotes mutant
T6036 1500-1510 NN denotes transcript
T6034 1511-1513 VBZ denotes is
T6038 1514-1522 JJ denotes unstable
T6039 1522-1523 . denotes .
T6040 1523-1790 sentence denotes PGC-1α protein was not detected in whole cell (Figure 1E) or nuclear protein extracts (unpublished data) isolated from BAT of PGC-1α−/− mice under basal conditions or in response to cold exposure, a condition known to markedly induce the expression of PGC-1α in BAT.
T6041 1524-1527 NN denotes PGC
T6043 1527-1528 HYPH denotes -
T6042 1528-1530 NN denotes 1α
T6044 1531-1538 NN denotes protein
T6046 1539-1542 VBD denotes was
T6047 1543-1546 RB denotes not
T6045 1547-1555 VBN denotes detected
T6048 1556-1558 IN denotes in
T6049 1559-1564 JJ denotes whole
T6050 1565-1569 NN denotes cell
T6052 1570-1571 -LRB- denotes (
T6054 1571-1577 NN denotes Figure
T6053 1578-1580 NN denotes 1E
T6055 1580-1581 -RRB- denotes )
T6056 1582-1584 CC denotes or
T6057 1585-1592 JJ denotes nuclear
T6058 1593-1600 NN denotes protein
T6051 1601-1609 NNS denotes extracts
T6059 1610-1611 -LRB- denotes (
T6061 1611-1622 JJ denotes unpublished
T6060 1623-1627 NNS denotes data
T6062 1627-1628 -RRB- denotes )
T6063 1629-1637 VBN denotes isolated
T6064 1638-1642 IN denotes from
T6065 1643-1646 NN denotes BAT
T6066 1647-1649 IN denotes of
T6067 1650-1653 NN denotes PGC
T6069 1653-1654 HYPH denotes -
T6068 1654-1656 NN denotes 1α
T6071 1656-1657 SYM denotes −
T6072 1657-1658 HYPH denotes /
T6073 1658-1659 SYM denotes −
T6070 1660-1664 NNS denotes mice
T6074 1665-1670 IN denotes under
T6075 1671-1676 JJ denotes basal
T6076 1677-1687 NNS denotes conditions
T6077 1688-1690 CC denotes or
T6078 1691-1693 IN denotes in
T6079 1694-1702 NN denotes response
T6080 1703-1705 IN denotes to
T6081 1706-1710 NN denotes cold
T6082 1711-1719 NN denotes exposure
T6083 1719-1721 , denotes ,
T6084 1721-1722 DT denotes a
T6085 1723-1732 NN denotes condition
T6086 1733-1738 VBN denotes known
T6087 1739-1741 TO denotes to
T6089 1742-1750 RB denotes markedly
T6088 1751-1757 VB denotes induce
T6090 1758-1761 DT denotes the
T6091 1762-1772 NN denotes expression
T6092 1773-1775 IN denotes of
T6093 1776-1779 NN denotes PGC
T6095 1779-1780 HYPH denotes -
T6094 1780-1782 NN denotes 1α
T6096 1783-1785 IN denotes in
T6097 1786-1789 NN denotes BAT
T6098 1789-1790 . denotes .
T6099 1790-1889 sentence denotes Smaller mutant PGC-1α proteins were also not detected by Western blot analysis (unpublished data).
T6100 1791-1798 JJR denotes Smaller
T6102 1799-1805 NN denotes mutant
T6103 1806-1809 NN denotes PGC
T6105 1809-1810 HYPH denotes -
T6104 1810-1812 NN denotes 1α
T6101 1813-1821 NN denotes proteins
T6107 1822-1826 VBD denotes were
T6108 1827-1831 RB denotes also
T6109 1832-1835 RB denotes not
T6106 1836-1844 VBN denotes detected
T6110 1845-1847 IN denotes by
T6111 1848-1855 NNP denotes Western
T6112 1856-1860 NN denotes blot
T6113 1861-1869 NN denotes analysis
T6114 1870-1871 -LRB- denotes (
T6116 1871-1882 JJ denotes unpublished
T6115 1883-1887 NNS denotes data
T6117 1887-1888 -RRB- denotes )
T6118 1888-1889 . denotes .
T6119 1889-2054 sentence denotes Lastly, expression of the genes encoding the other known PGC-1 family members, PGC-1β and PRC, was not significantly altered in heart of PGC-1α−/− mice (Figure 1C).
T6120 1890-1896 RB denotes Lastly
T6122 1896-1898 , denotes ,
T6123 1898-1908 NN denotes expression
T6124 1909-1911 IN denotes of
T6125 1912-1915 DT denotes the
T6126 1916-1921 NNS denotes genes
T6127 1922-1930 VBG denotes encoding
T6128 1931-1934 DT denotes the
T6130 1935-1940 JJ denotes other
T6131 1941-1946 JJ denotes known
T6132 1947-1950 NN denotes PGC
T6133 1950-1951 HYPH denotes -
T6134 1951-1952 CD denotes 1
T6135 1953-1959 NN denotes family
T6129 1960-1967 NNS denotes members
T6136 1967-1969 , denotes ,
T6137 1969-1972 NN denotes PGC
T6139 1972-1973 HYPH denotes -
T6138 1973-1975 NN denotes 1β
T6140 1976-1979 CC denotes and
T6141 1980-1983 NN denotes PRC
T6142 1983-1985 , denotes ,
T6143 1985-1988 VBD denotes was
T6144 1989-1992 RB denotes not
T6145 1993-2006 RB denotes significantly
T6121 2007-2014 VBN denotes altered
T6146 2015-2017 IN denotes in
T6147 2018-2023 NN denotes heart
T6148 2024-2026 IN denotes of
T6149 2027-2030 NN denotes PGC
T6151 2030-2031 HYPH denotes -
T6150 2031-2033 NN denotes 1α
T6153 2033-2034 SYM denotes −
T6154 2034-2035 HYPH denotes /
T6155 2035-2036 SYM denotes −
T6152 2037-2041 NNS denotes mice
T6156 2042-2043 -LRB- denotes (
T6158 2043-2049 NN denotes Figure
T6157 2050-2052 NN denotes 1C
T6159 2052-2053 -RRB- denotes )
T6160 2053-2054 . denotes .
T6161 2054-2171 sentence denotes Taken together, these results support the conclusion that the gene targeting event resulted in a PGC-1α null allele.
T6162 2055-2060 VBN denotes Taken
T6164 2061-2069 RB denotes together
T6165 2069-2071 , denotes ,
T6166 2071-2076 DT denotes these
T6167 2077-2084 NNS denotes results
T6163 2085-2092 VBP denotes support
T6168 2093-2096 DT denotes the
T6169 2097-2107 NN denotes conclusion
T6170 2108-2112 IN denotes that
T6172 2113-2116 DT denotes the
T6174 2117-2121 NN denotes gene
T6175 2122-2131 VBG denotes targeting
T6173 2132-2137 NN denotes event
T6171 2138-2146 VBD denotes resulted
T6176 2147-2149 IN denotes in
T6177 2150-2151 DT denotes a
T6179 2152-2155 NN denotes PGC
T6181 2155-2156 HYPH denotes -
T6180 2156-2158 NN denotes 1α
T6182 2159-2163 JJ denotes null
T6178 2164-2170 NN denotes allele
T6183 2170-2171 . denotes .
R1125 T5691 T5692 det A,vector
R1126 T5692 T5698 nsubjpass vector,generated
R1127 T5693 T5694 npadvmod neomycin,based
R1128 T5694 T5692 amod based,vector
R1129 T5695 T5694 punct -,based
R1130 T5696 T5692 compound gene,vector
R1131 T5697 T5692 compound targeting,vector
R1132 T5699 T5698 auxpass was,generated
R1133 T5700 T5701 aux to,delete
R1134 T5701 T5698 advcl delete,generated
R1135 T5702 T5703 nmod exons,4
R1136 T5703 T5701 dobj 4,delete
R1137 T5704 T5703 cc and,4
R1138 T5705 T5703 conj 5,4
R1139 T5706 T5703 prep of,4
R1140 T5707 T5708 det the,gene
R1141 T5708 T5706 pobj gene,of
R1142 T5709 T5708 amod murine,gene
R1143 T5710 T5711 compound PGC,1α
R1144 T5711 T5708 compound 1α,gene
R1145 T5712 T5711 punct -,1α
R1146 T5713 T5698 punct .,generated
R1147 T5715 T5716 det The,event
R1148 T5716 T5718 nsubj event,resulted
R1149 T5717 T5716 compound targeting,event
R1150 T5719 T5718 prep in,resulted
R1151 T5720 T5721 det a,recombination
R1152 T5721 T5719 pobj recombination,in
R1153 T5722 T5721 nummod 3,recombination
R1154 T5723 T5722 punct ′,3
R1155 T5724 T5721 amod homologous,recombination
R1156 T5725 T5718 prep with,resulted
R1157 T5726 T5725 pobj insertion,with
R1158 T5727 T5726 prep of,insertion
R1159 T5728 T5729 det the,remainder
R1160 T5729 T5727 pobj remainder,of
R1161 T5730 T5729 prep of,remainder
R1162 T5731 T5732 det the,construct
R1163 T5732 T5730 pobj construct,of
R1164 T5733 T5734 punct (,1A
R1165 T5734 T5718 parataxis 1A,resulted
R1166 T5735 T5734 compound Figure,1A
R1167 T5736 T5734 punct ),1A
R1168 T5737 T5718 punct .,resulted
R1169 T5739 T5740 det The,event
R1170 T5740 T5744 nsubjpass event,confirmed
R1171 T5741 T5742 compound insertion,recombination
R1172 T5742 T5740 compound recombination,event
R1173 T5743 T5742 punct /,recombination
R1174 T5745 T5744 auxpass was,confirmed
R1175 T5746 T5744 prep by,confirmed
R1176 T5747 T5748 compound Southern,blotting
R1177 T5748 T5746 pobj blotting,by
R1178 T5749 T5748 cc and,blotting
R1179 T5750 T5751 compound DNA,sequencing
R1180 T5751 T5748 conj sequencing,blotting
R1181 T5752 T5744 punct .,confirmed
R1182 T5754 T5755 det The,insertion
R1183 T5755 T5756 nsubj insertion,caused
R1184 T5757 T5758 det an,duplication
R1185 T5758 T5756 dobj duplication,caused
R1186 T5759 T5758 nmod exon,duplication
R1187 T5760 T5759 nummod 3,exon
R1188 T5761 T5758 prep between,duplication
R1189 T5762 T5763 nmod exons,5
R1190 T5763 T5761 pobj 5,between
R1191 T5764 T5763 cc and,5
R1192 T5765 T5763 conj 6,5
R1193 T5766 T5767 dep that,creates
R1194 T5767 T5758 relcl creates,duplication
R1195 T5768 T5769 det a,frameshift
R1196 T5769 T5767 dobj frameshift,creates
R1197 T5770 T5771 amod coding,region
R1198 T5771 T5769 compound region,frameshift
R1199 T5772 T5767 advcl resulting,creates
R1200 T5773 T5772 prep in,resulting
R1201 T5774 T5775 det a,termination
R1202 T5775 T5773 pobj termination,in
R1203 T5776 T5775 amod premature,termination
R1204 T5777 T5775 prep at,termination
R1205 T5778 T5779 compound amino,acid
R1206 T5779 T5777 pobj acid,at
R1207 T5780 T5779 nummod 255,acid
R1208 T5781 T5756 punct .,caused
R1209 T5783 T5784 compound Germline,transmission
R1210 T5784 T5785 nsubjpass transmission,confirmed
R1211 T5786 T5784 prep of,transmission
R1212 T5787 T5788 det the,allele
R1213 T5788 T5786 pobj allele,of
R1214 T5789 T5788 compound mutant,allele
R1215 T5790 T5785 auxpass was,confirmed
R1216 T5791 T5785 advcl using,confirmed
R1217 T5792 T5793 compound Southern,blotting
R1218 T5793 T5791 dobj blotting,using
R1219 T5794 T5795 punct (,1B
R1220 T5795 T5793 parataxis 1B,blotting
R1221 T5796 T5795 compound Figure,1B
R1222 T5797 T5795 punct ),1B
R1223 T5798 T5793 cc and,blotting
R1224 T5799 T5793 conj PCR,blotting
R1225 T5800 T5801 punct (,data
R1226 T5801 T5799 meta data,PCR
R1227 T5802 T5801 amod unpublished,data
R1228 T5803 T5801 punct ),data
R1229 T5804 T5785 punct .,confirmed
R1230 T5806 T5807 det The,disruption
R1231 T5807 T5812 nsubj disruption,resulted
R1232 T5808 T5809 compound PGC,1α
R1233 T5809 T5807 compound 1α,disruption
R1234 T5810 T5809 punct -,1α
R1235 T5811 T5807 compound gene,disruption
R1236 T5813 T5812 prep in,resulted
R1237 T5814 T5815 det an,transcript
R1238 T5815 T5813 pobj transcript,in
R1239 T5816 T5815 amod unstable,transcript
R1240 T5817 T5818 dep that,detected
R1241 T5818 T5815 relcl detected,transcript
R1242 T5819 T5818 aux could,detected
R1243 T5820 T5818 neg not,detected
R1244 T5821 T5818 auxpass be,detected
R1245 T5822 T5818 prep by,detected
R1246 T5823 T5824 compound RNA,analysis
R1247 T5824 T5822 pobj analysis,by
R1248 T5825 T5824 compound blot,analysis
R1249 T5826 T5818 prep in,detected
R1250 T5827 T5826 pobj heart,in
R1251 T5828 T5827 cc and,heart
R1252 T5829 T5830 amod other,tissues
R1253 T5830 T5827 conj tissues,heart
R1254 T5831 T5818 prep in,detected
R1255 T5832 T5833 nmod PGC,1α
R1256 T5833 T5835 nmod 1α,mice
R1257 T5834 T5833 punct -,1α
R1258 T5835 T5831 pobj mice,in
R1259 T5836 T5833 punct −,1α
R1260 T5837 T5833 punct /,1α
R1261 T5838 T5833 punct −,1α
R1262 T5839 T5840 punct (,1C
R1263 T5840 T5812 parataxis 1C,resulted
R1264 T5841 T5840 compound Figure,1C
R1265 T5842 T5840 cc and,1C
R1266 T5843 T5844 amod unpublished,data
R1267 T5844 T5840 conj data,1C
R1268 T5845 T5840 punct ),1C
R1269 T5846 T5812 punct .,resulted
R1270 T5848 T5849 amod Quantitative,PCR
R1271 T5849 T5852 nsubjpass PCR,utilized
R1272 T5850 T5849 compound RT,PCR
R1273 T5851 T5849 punct -,PCR
R1274 T5853 T5852 auxpass was,utilized
R1275 T5854 T5855 aux to,evaluate
R1276 T5855 T5852 advcl evaluate,utilized
R1277 T5856 T5855 advmod further,evaluate
R1278 T5857 T5858 det the,efficacy
R1279 T5858 T5855 dobj efficacy,evaluate
R1280 T5859 T5858 prep of,efficacy
R1281 T5860 T5861 det the,targeting
R1282 T5861 T5859 pobj targeting,of
R1283 T5862 T5861 compound gene,targeting
R1284 T5863 T5852 punct .,utilized
R1285 T5865 T5866 prep For,designed
R1286 T5867 T5868 det these,studies
R1287 T5868 T5865 pobj studies,For
R1288 T5869 T5866 punct ", ",designed
R1289 T5870 T5871 compound PCR,primers
R1290 T5871 T5866 nsubjpass primers,designed
R1291 T5872 T5866 auxpass were,designed
R1292 T5873 T5874 aux to,amplify
R1293 T5874 T5866 advcl amplify,designed
R1294 T5875 T5876 det a,region
R1295 T5876 T5874 dobj region,amplify
R1296 T5877 T5876 prep of,region
R1297 T5878 T5879 det the,transcript
R1298 T5879 T5877 pobj transcript,of
R1299 T5880 T5881 compound PGC,1α
R1300 T5881 T5879 compound 1α,transcript
R1301 T5882 T5881 punct -,1α
R1302 T5883 T5879 compound gene,transcript
R1303 T5884 T5876 acl containing,region
R1304 T5885 T5886 det the,border
R1305 T5886 T5884 dobj border,containing
R1306 T5887 T5888 nmod exon,5
R1307 T5888 T5886 nummod 5,border
R1308 T5889 T5890 punct –,6
R1309 T5890 T5888 prep 6,5
R1310 T5891 T5886 punct (,border
R1311 T5892 T5886 acl predicted,border
R1312 T5893 T5894 aux to,be
R1313 T5894 T5892 xcomp be,predicted
R1314 T5895 T5894 acomp absent,be
R1315 T5896 T5894 prep in,be
R1316 T5897 T5898 nmod PGC,1α
R1317 T5898 T5900 nmod 1α,mice
R1318 T5899 T5898 punct -,1α
R1319 T5900 T5896 pobj mice,in
R1320 T5901 T5898 punct −,1α
R1321 T5902 T5898 punct /,1α
R1322 T5903 T5898 punct −,1α
R1323 T5904 T5886 punct ),border
R1324 T5905 T5886 cc or,border
R1325 T5906 T5907 det the,border
R1326 T5907 T5886 conj border,border
R1327 T5908 T5909 nmod exon,5
R1328 T5909 T5907 nummod 5,border
R1329 T5910 T5911 punct –,3
R1330 T5911 T5909 prep 3,5
R1331 T5912 T5907 punct (,border
R1332 T5913 T5907 acl predicted,border
R1333 T5914 T5915 aux to,be
R1334 T5915 T5913 xcomp be,predicted
R1335 T5916 T5915 acomp present,be
R1336 T5917 T5918 advmod only,in
R1337 T5918 T5915 prep in,be
R1338 T5919 T5920 det the,mice
R1339 T5920 T5918 pobj mice,in
R1340 T5921 T5922 nmod PGC,1α
R1341 T5922 T5920 nmod 1α,mice
R1342 T5923 T5922 punct -,1α
R1343 T5924 T5922 punct −,1α
R1344 T5925 T5922 punct /,1α
R1345 T5926 T5922 punct −,1α
R1346 T5927 T5907 punct ),border
R1347 T5928 T5866 punct .,designed
R1348 T5930 T5931 det The,amplicon
R1349 T5931 T5936 nsubjpass amplicon,detected
R1350 T5932 T5933 nmod exon,5
R1351 T5933 T5931 nummod 5,amplicon
R1352 T5934 T5935 punct –,6
R1353 T5935 T5933 prep 6,5
R1354 T5937 T5936 auxpass was,detected
R1355 T5938 T5936 prep in,detected
R1356 T5939 T5938 pobj heart,in
R1357 T5940 T5939 cc and,heart
R1358 T5941 T5939 conj BAT,heart
R1359 T5942 T5939 prep of,heart
R1360 T5943 T5944 amod wild,type
R1361 T5944 T5946 nmod type,mice
R1362 T5945 T5944 punct -,type
R1363 T5946 T5942 pobj mice,of
R1364 T5947 T5944 punct (,type
R1365 T5948 T5944 appos WT,type
R1366 T5949 T5944 punct ),type
R1367 T5950 T5944 cc but,type
R1368 T5951 T5950 neg not,but
R1369 T5952 T5953 compound PGC,1α
R1370 T5953 T5944 conj 1α,type
R1371 T5954 T5953 punct -,1α
R1372 T5955 T5953 punct −,1α
R1373 T5956 T5953 punct /,1α
R1374 T5957 T5953 punct −,1α
R1375 T5958 T5959 punct (,1D
R1376 T5959 T5936 parataxis 1D,detected
R1377 T5960 T5959 compound Figure,1D
R1378 T5961 T5959 punct ),1D
R1379 T5962 T5936 punct .,detected
R1380 T5964 T5965 advmod Conversely,was
R1381 T5966 T5965 punct ", ",was
R1382 T5967 T5968 det the,product
R1383 T5968 T5965 nsubj product,was
R1384 T5969 T5970 nmod exon,5
R1385 T5970 T5968 nummod 5,product
R1386 T5971 T5972 punct –,3
R1387 T5972 T5970 prep 3,5
R1388 T5973 T5965 acomp present,was
R1389 T5974 T5975 advmod only,in
R1390 T5975 T5965 prep in,was
R1391 T5976 T5977 nmod PGC,1α
R1392 T5977 T5979 nmod 1α,mice
R1393 T5978 T5977 punct -,1α
R1394 T5979 T5975 pobj mice,in
R1395 T5980 T5977 punct −,1α
R1396 T5981 T5977 punct /,1α
R1397 T5982 T5977 punct −,1α
R1398 T5983 T5984 punct (,1D
R1399 T5984 T5965 parataxis 1D,was
R1400 T5985 T5984 compound Figure,1D
R1401 T5986 T5984 punct ),1D
R1402 T5987 T5965 punct .,was
R1403 T5989 T5990 det An,amplicon
R1404 T5990 T5996 nsubjpass amplicon,detected
R1405 T5991 T5992 nmod exon,10
R1406 T5992 T5990 nummod 10,amplicon
R1407 T5993 T5994 punct –,11
R1408 T5994 T5992 prep 11,10
R1409 T5995 T5990 compound border,amplicon
R1410 T5997 T5990 punct (,amplicon
R1411 T5998 T5990 acl predicted,amplicon
R1412 T5999 T6000 aux to,be
R1413 T6105 T6104 punct -,1α
R1414 T6107 T6106 auxpass were,detected
R1415 T6108 T6106 advmod also,detected
R1416 T6109 T6106 neg not,detected
R1417 T6110 T6106 prep by,detected
R1418 T6111 T6112 compound Western,blot
R1419 T6112 T6113 compound blot,analysis
R1420 T6113 T6110 pobj analysis,by
R1421 T6114 T6115 punct (,data
R1422 T6115 T6106 meta data,detected
R1423 T6116 T6115 amod unpublished,data
R1424 T6117 T6115 punct ),data
R1425 T6118 T6106 punct .,detected
R1426 T6120 T6121 advmod Lastly,altered
R1427 T6000 T5998 xcomp be,predicted
R1428 T6122 T6121 punct ", ",altered
R1429 T6001 T6000 acomp present,be
R1430 T6002 T6000 prep in,be
R1431 T6003 T6004 det both,genotypes
R1432 T6123 T6121 nsubjpass expression,altered
R1433 T6004 T6002 pobj genotypes,in
R1434 T6005 T5996 punct ),detected
R1435 T6006 T5996 auxpass was,detected
R1436 T6007 T5996 prep in,detected
R1437 T6008 T6009 nmod WT,mice
R1438 T6124 T6123 prep of,expression
R1439 T6009 T6007 pobj mice,in
R1440 T6010 T6008 cc and,WT
R1441 T6011 T6012 compound PGC,1α
R1442 T6125 T6126 det the,genes
R1443 T6012 T6008 conj 1α,WT
R1444 T6013 T6012 punct -,1α
R1445 T6014 T6012 punct −,1α
R1446 T6126 T6124 pobj genes,of
R1447 T6015 T6012 punct /,1α
R1448 T6016 T6012 punct −,1α
R1449 T6017 T5996 punct ", ",detected
R1450 T6127 T6126 acl encoding,genes
R1451 T6018 T5996 cc but,detected
R1452 T6019 T6020 auxpass was,diminished
R1453 T6020 T5996 conj diminished,detected
R1454 T6128 T6129 det the,members
R1455 T6021 T6020 advmod greatly,diminished
R1456 T6022 T6020 prep in,diminished
R1457 T6023 T6024 det the,mice
R1458 T6129 T6127 dobj members,encoding
R1459 T6024 T6022 pobj mice,in
R1460 T6025 T6026 nmod PGC,1α
R1461 T6130 T6129 amod other,members
R1462 T6026 T6024 nmod 1α,mice
R1463 T6027 T6026 punct -,1α
R1464 T6028 T6026 punct −,1α
R1465 T6131 T6129 amod known,members
R1466 T6029 T6026 punct /,1α
R1467 T6132 T6129 nmod PGC,members
R1468 T6030 T6026 punct −,1α
R1469 T6031 T6020 punct ", ",diminished
R1470 T6032 T6020 advcl indicating,diminished
R1471 T6033 T6034 mark that,is
R1472 T6133 T6132 punct -,PGC
R1473 T6134 T6132 nummod 1,PGC
R1474 T6034 T6032 ccomp is,indicating
R1475 T6035 T6036 det the,transcript
R1476 T6135 T6129 compound family,members
R1477 T6036 T6034 nsubj transcript,is
R1478 T6037 T6036 compound mutant,transcript
R1479 T6038 T6034 acomp unstable,is
R1480 T6136 T6129 punct ", ",members
R1481 T6039 T5996 punct .,detected
R1482 T6137 T6138 compound PGC,1β
R1483 T6041 T6042 compound PGC,1α
R1484 T6042 T6044 compound 1α,protein
R1485 T6043 T6042 punct -,1α
R1486 T6138 T6129 appos 1β,members
R1487 T6044 T6045 nsubjpass protein,detected
R1488 T6046 T6045 auxpass was,detected
R1489 T6139 T6138 punct -,1β
R1490 T6047 T6045 neg not,detected
R1491 T6048 T6045 prep in,detected
R1492 T6140 T6138 cc and,1β
R1493 T6049 T6050 amod whole,cell
R1494 T6050 T6051 nmod cell,extracts
R1495 T6051 T6048 pobj extracts,in
R1496 T6141 T6138 conj PRC,1β
R1497 T6052 T6053 punct (,1E
R1498 T6053 T6050 parataxis 1E,cell
R1499 T6142 T6121 punct ", ",altered
R1500 T6054 T6053 compound Figure,1E
R1501 T6055 T6053 punct ),1E
R1502 T6056 T6050 cc or,cell
R1503 T6143 T6121 auxpass was,altered
R1504 T6057 T6058 amod nuclear,protein
R1505 T6058 T6050 conj protein,cell
R1506 T6059 T6060 punct (,data
R1507 T6144 T6121 neg not,altered
R1508 T6060 T6051 meta data,extracts
R1509 T6061 T6060 amod unpublished,data
R1510 T6145 T6121 advmod significantly,altered
R1511 T6062 T6060 punct ),data
R1512 T6063 T6051 acl isolated,extracts
R1513 T6146 T6121 prep in,altered
R1514 T6064 T6063 prep from,isolated
R1515 T6065 T6064 pobj BAT,from
R1516 T6066 T6065 prep of,BAT
R1517 T6147 T6146 pobj heart,in
R1518 T6067 T6068 nmod PGC,1α
R1519 T6068 T6070 nmod 1α,mice
R1520 T6148 T6147 prep of,heart
R1521 T6069 T6068 punct -,1α
R1522 T6070 T6066 pobj mice,of
R1523 T6071 T6068 punct −,1α
R1524 T6072 T6068 punct /,1α
R1525 T6073 T6068 punct −,1α
R1526 T6149 T6150 nmod PGC,1α
R1527 T6074 T6063 prep under,isolated
R1528 T6075 T6076 amod basal,conditions
R1529 T6076 T6074 pobj conditions,under
R1530 T6150 T6152 nmod 1α,mice
R1531 T6077 T6045 cc or,detected
R1532 T6078 T6045 conj in,detected
R1533 T6079 T6078 pobj response,in
R1534 T6151 T6150 punct -,1α
R1535 T6080 T6079 prep to,response
R1536 T6081 T6082 compound cold,exposure
R1537 T6082 T6080 pobj exposure,to
R1538 T6152 T6148 pobj mice,of
R1539 T6083 T6082 punct ", ",exposure
R1540 T6084 T6085 det a,condition
R1541 T6085 T6082 appos condition,exposure
R1542 T6153 T6150 punct −,1α
R1543 T6086 T6085 acl known,condition
R1544 T6087 T6088 aux to,induce
R1545 T6154 T6150 punct /,1α
R1546 T6088 T6086 xcomp induce,known
R1547 T6089 T6088 advmod markedly,induce
R1548 T6155 T6150 punct −,1α
R1549 T6090 T6091 det the,expression
R1550 T6091 T6088 dobj expression,induce
R1551 T6092 T6091 prep of,expression
R1552 T6156 T6157 punct (,1C
R1553 T6093 T6094 compound PGC,1α
R1554 T6094 T6092 pobj 1α,of
R1555 T6095 T6094 punct -,1α
R1556 T6096 T6088 prep in,induce
R1557 T6157 T6121 parataxis 1C,altered
R1558 T6097 T6096 pobj BAT,in
R1559 T6098 T6045 punct .,detected
R1560 T6158 T6157 compound Figure,1C
R1561 T6100 T6101 amod Smaller,proteins
R1562 T6101 T6106 nsubjpass proteins,detected
R1563 T6102 T6101 compound mutant,proteins
R1564 T6103 T6104 compound PGC,1α
R1565 T6159 T6157 punct ),1C
R1566 T6104 T6101 compound 1α,proteins
R1567 T6160 T6121 punct .,altered
R1568 T6162 T6163 advcl Taken,support
R1569 T6164 T6162 advmod together,Taken
R1570 T6165 T6163 punct ", ",support
R1571 T6166 T6167 det these,results
R1572 T6167 T6163 nsubj results,support
R1573 T6168 T6169 det the,conclusion
R1574 T6169 T6163 dobj conclusion,support
R1575 T6170 T6171 mark that,resulted
R1576 T6171 T6169 acl resulted,conclusion
R1577 T6172 T6173 det the,event
R1578 T6173 T6171 nsubj event,resulted
R1579 T6174 T6173 nmod gene,event
R1580 T6175 T6174 amod targeting,gene
R1581 T6176 T6171 prep in,resulted
R1582 T6177 T6178 det a,allele
R1583 T6178 T6176 pobj allele,in
R1584 T6179 T6180 compound PGC,1α
R1585 T6180 T6182 npadvmod 1α,null
R1586 T6181 T6180 punct -,1α
R1587 T6182 T6178 amod null,allele
R1588 T6183 T6163 punct .,support