bionlp-st-ge-2016-test-ihmc  

PMC:3317373 / 5456-7257 JSONTXT 9 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T3039 63-73 Entity denotes astrocytes
T3044 136-182 Entity denotes The cell pellet obtained by centrifugation was
T3093 216-227 Entity denotes 10 mM HEPES
T3080 229-231 Protein denotes pH
T3043 238-273 Entity denotes 10 mM KCl, 0.1 mM EDTA, 0.1 mM EGTA
T3083 244-248 Entity denotes KCl,
T3091 256-261 Entity denotes EDTA,
T3094 275-286 Entity denotes 1.0 mM DTT,
T3076 291-327 Entity denotes 0.5 mM phenylmethylsulfonyl fluoride
T3054 334-350 Entity denotes 10% Nonidet P-40
T3060 365-373 Entity denotes vortexed
T3081 387-397 Entity denotes the nuclei
T3059 431-451 Protein denotes The nuclear proteins
T3095 431-501 Negative_regulation denotes The nuclear proteins were extracted with buffer containing 20 mM HEPES
T3040 490-501 Entity denotes 20 mM HEPES
T3068 503-505 Protein denotes pH
T3063 512-537 Entity denotes 0.4 mM NaCl, 1.0 mM EDTA,
T3042 525-536 Entity denotes 1.0 mM EDTA
T3075 538-562 Entity denotes 1.0 mM EGTA, 1.0 mM DTT,
T3041 551-561 Entity denotes 1.0 mM DTT
T3070 567-603 Entity denotes 1.0 mM phenylmethylsulfonyl fluoride
T3072 672-719 Entity denotes the supernatant containing the nuclear proteins
T3062 699-719 Protein denotes the nuclear proteins
T3086 751-776 Entity denotes Protein concentration was
T3057 794-824 Entity denotes a bicinchoninic acid assay kit
T3092 796-814 Entity denotes bicinchoninic acid
T3082 830-850 Protein denotes bovine serum albumin
T3061 868-887 Entity denotes Pierce Biochemicals
T3090 889-907 Entity denotes Rockford, IL, USA)
T3050 934-937 Entity denotes gel
T3065 986-1048 Entity denotes Consensus oligonucleotide probe (5′-AGTTGAGGGGACTTTCCCAGGG-3′)
T3074 1068-1092 Entity denotes T4-polynucleotide kinase
T3056 1068-1092 Entity denotes T4-polynucleotide kinase
T3058 1071-1092 Protein denotes polynucleotide kinase
T3055 1106-1126 Entity denotes -ATP (Free Biotech.,
T3067 1144-1159 Protein denotes Nuclear protein
T3089 1220-1234 Entity denotes 10 mM Tris-HCl
T3046 1220-1234 Entity denotes 10 mM Tris-HCl
T3073 1236-1238 Protein denotes PH
T3084 1257-1282 Entity denotes 0.5 mM NaCl, 4% glycerol,
T3071 1270-1281 Entity denotes 4% glycerol
T3078 1283-1307 Entity denotes 0.5 mM EDTA, 0.5 mM DTT,
T3087 1296-1306 Entity denotes 0.5 mM DTT
T3088 1317-1321 Entity denotes poly
T3053 1332-1349 Entity denotes 20 minutes on ice
T3064 1366-1410 Entity denotes of the 1 μL 32P-labled oligonucleotide probe
T3045 1445-1462 Entity denotes 20 minutes on ice
T3085 1464-1489 Entity denotes The DNA-protein complexes
T3079 1527-1562 Entity denotes 4% nondenaturing polyacrylamide gel
T3049 1544-1558 Entity denotes polyacrylamide
T3069 1572-1601 Entity denotes TBE buffer (tris-borate-EDTA)
T3051 1584-1588 Entity denotes tris
T3048 1584-1600 Entity denotes tris-borate-EDTA
T3066 1584-1600 Entity denotes tris-borate-EDTA
T3047 1589-1595 Entity denotes borate
T3077 1653-1660 Entity denotes the gel
T3052 1739-1744 Protein denotes NF-κB
R2676 T3059 T3095 themeOf The nuclear proteins,The nuclear proteins were extracted with buffer containing 20 mM HEPES