Id |
Subject |
Object |
Predicate |
Lexical cue |
T3988 |
19-36 |
Protein |
denotes |
Cellular proteins |
T3974 |
103-106 |
Protein |
denotes |
SDS |
T3977 |
103-141 |
Entity |
denotes |
SDS-polyacrylamide gel and transferred |
T3979 |
107-121 |
Entity |
denotes |
polyacrylamide |
T3983 |
147-188 |
Entity |
denotes |
Hybond ECL membranes (Amersham Pharmacia, |
T3980 |
154-157 |
Protein |
denotes |
ECL |
T3975 |
206-223 |
Entity |
denotes |
The ECL membranes |
T3984 |
210-213 |
Protein |
denotes |
ECL |
T3976 |
244-276 |
Protein |
denotes |
the appropriate primary antibody |
T3985 |
321-363 |
Entity |
denotes |
peroxidase-conjugated secondary antibodies |
T3978 |
321-363 |
Protein |
denotes |
peroxidase-conjugated secondary antibodies |
T3982 |
332-363 |
Entity |
denotes |
conjugated secondary antibodies |
T3986 |
425-428 |
Entity |
denotes |
ECL |
T3987 |
458-476 |
Entity |
denotes |
The same membranes |
T3981 |
494-504 |
Protein |
denotes |
anti-GAPDH |
T3973 |
506-558 |
Protein |
denotes |
glyceraldehyde-3-phosphate dehydrogenase, Biodesign, |