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PMC:3342329 / 33711-34891 JSONTXT 23 Projects

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Id Subject Object Predicate Lexical cue
T16470 0-11 NNPS denotes Lymphocytes
T16471 12-16 VBD denotes were
T16472 17-25 VBN denotes obtained
T16473 26-30 IN denotes from
T16474 31-36 NN denotes tumor
T16475 37-40 CC denotes and
T16476 41-47 NN denotes spleen
T16477 48-55 NNS denotes tissues
T16478 56-59 IN denotes for
T16479 60-69 NN denotes phenotype
T16480 70-78 NN denotes analysis
T16481 78-79 . denotes .
T16482 80-86 NNP denotes Spleen
T16483 87-90 CC denotes and
T16484 91-96 NN denotes tumor
T16485 97-108 NNS denotes lymphocytes
T16486 109-113 VBD denotes were
T16487 114-122 VBN denotes isolated
T16488 123-127 IN denotes from
T16489 128-133 JJ denotes fresh
T16490 134-140 NN denotes spleen
T16491 141-144 CC denotes and
T16492 145-150 NN denotes tumor
T16493 151-159 NNS denotes biopsies
T16494 160-168 VBN denotes obtained
T16495 169-173 IN denotes from
T16496 174-179 CD denotes three
T16497 180-184 NNS denotes mice
T16498 185-188 IN denotes per
T16499 189-194 NN denotes group
T16500 194-195 . denotes .
T16501 196-202 NNP denotes Spleen
T16502 203-206 CC denotes and
T16503 207-212 NN denotes tumor
T16504 213-221 NNS denotes biopsies
T16505 222-226 VBD denotes were
T16506 227-234 RB denotes briefly
T16507 235-246 VBN denotes homogenized
T16508 247-259 RB denotes mechanically
T16509 260-262 IN denotes in
T16510 263-266 NNP denotes PBS
T16511 266-267 , denotes ,
T16512 268-276 VBN denotes filtered
T16513 277-278 -LRB- denotes (
T16514 278-281 CD denotes 100
T16515 282-284 NN denotes µm
T16516 285-289 NN denotes cell
T16517 290-298 NN denotes strainer
T16518 298-299 : denotes ;
T16519 300-302 NNP denotes BD
T16520 303-313 NNP denotes PharMingen
T16521 313-314 -RRB- denotes )
T16522 315-318 CC denotes and
T16523 319-323 RB denotes then
T16524 324-330 VBN denotes placed
T16525 331-333 IN denotes in
T16526 334-339 NN denotes lysis
T16527 340-346 NN denotes buffer
T16528 347-348 -LRB- denotes (
T16529 348-351 NNP denotes ACK
T16530 352-358 VBG denotes lysing
T16531 359-365 NN denotes buffer
T16532 365-366 , denotes ,
T16533 367-372 NNP denotes LONZA
T16534 372-373 -RRB- denotes )
T16535 374-376 TO denotes to
T16536 377-383 VB denotes remove
T16537 384-387 JJ denotes red
T16538 388-393 NN denotes blood
T16539 394-399 NNS denotes cells
T16540 399-400 . denotes .
T16541 401-404 CD denotes One
T16542 405-412 CD denotes million
T16543 413-424 NNS denotes splenocytes
T16544 425-429 VBD denotes were
T16545 430-436 VBN denotes washed
T16546 437-441 RB denotes once
T16547 442-444 IN denotes in
T16548 445-448 NNP denotes PBS
T16549 449-459 VBG denotes containing
T16550 460-461 CD denotes 1
T16551 461-462 NN denotes %
T16552 463-469 JJ denotes bovine
T16553 470-475 NN denotes serum
T16554 476-483 NN denotes albumin
T16555 484-485 -LRB- denotes (
T16556 485-488 NNP denotes BSA
T16557 488-489 -RRB- denotes )
T16558 490-493 CC denotes and
T16559 494-506 JJ denotes re-suspended
T16560 507-509 IN denotes in
T16561 510-513 CD denotes 100
T16562 514-516 NN denotes µl
T16563 517-519 IN denotes of
T16564 520-527 NNP denotes PBS/BSA
T16565 528-534 NN denotes buffer
T16566 534-535 . denotes .
T16567 536-545 RB denotes Meanwhile
T16568 545-546 , denotes ,
T16569 547-550 CD denotes one
T16570 551-558 CD denotes million
T16571 559-564 NNS denotes cells
T16572 565-573 VBN denotes obtained
T16573 574-578 IN denotes from
T16574 579-584 NN denotes tumor
T16575 585-592 NNS denotes tissues
T16576 593-597 VBD denotes were
T16577 598-604 VBN denotes placed
T16578 605-607 IN denotes in
T16579 608-612 NNP denotes RPMI
T16580 613-619 NN denotes medium
T16581 619-620 , denotes ,
T16582 621-630 VBN denotes incubated
T16583 631-633 TO denotes to
T16584 634-640 VB denotes obtain
T16585 641-651 NN denotes suspension
T16586 652-656 NN denotes cell
T16587 657-660 IN denotes for
T16588 661-666 CD denotes 1hour
T16589 667-669 IN denotes at
T16590 670-672 CD denotes 37
T16591 672-673 CD denotes °
T16592 673-674 NNP denotes C
T16593 674-675 , denotes ,
T16594 676-687 VBN denotes centrifuged
T16595 688-689 -LRB- denotes (
T16596 689-693 CD denotes 1200
T16597 694-697 NN denotes rpm
T16598 697-698 , denotes ,
T16599 699-700 CD denotes 3
T16600 701-704 NN denotes min
T16601 704-705 -RRB- denotes )
T16602 706-709 CC denotes and
T16603 710-714 RB denotes then
T16604 715-721 VBD denotes washed
T16605 722-724 IN denotes in
T16606 725-728 NNP denotes PBS
T16607 728-729 . denotes .
T16608 730-734 JJ denotes Next
T16609 734-735 , denotes ,
T16610 736-739 DT denotes the
T16611 740-751 NNS denotes lymphocytes
T16612 752-756 VBD denotes were
T16613 757-766 VBN denotes incubated
T16614 767-771 IN denotes with
T16615 772-779 JJ denotes various
T16616 780-790 VBN denotes conjugated
T16617 791-801 JJ denotes monoclonal
T16618 802-812 NNS denotes antibodies
T16619 812-813 , denotes ,
T16620 814-823 VBG denotes including
T16621 824-831 NNP denotes CD4-APC
T16622 831-832 , denotes ,
T16623 833-841 NNP denotes CD8-FITC
T16624 842-845 CC denotes and
T16625 846-853 NNP denotes CD19-PE
T16626 854-857 IN denotes for
T16627 858-863 CD denotes 20min
T16628 864-866 IN denotes at
T16629 867-868 CD denotes 4
T16630 868-869 CD denotes °
T16631 869-870 NNP denotes C
T16632 870-871 , denotes ,
T16633 872-878 VBD denotes washed
T16634 879-884 RB denotes twice
T16635 885-887 IN denotes in
T16636 888-891 NNP denotes PBS
T16637 891-892 , denotes ,
T16638 893-896 CC denotes and
T16639 897-909 JJ denotes re-suspended
T16640 910-912 IN denotes in
T16641 913-916 CD denotes 400
T16642 917-919 NN denotes µl
T16643 920-922 IN denotes of
T16644 923-926 NNP denotes PBS
T16645 926-927 . denotes .
T16646 928-929 DT denotes A
T16647 930-934 NN denotes flow
T16648 935-945 JJ denotes cytometric
T16649 946-954 NN denotes analysis
T16650 955-958 VBD denotes was
T16651 959-968 VBN denotes performed
T16652 969-971 IN denotes on
T16653 972-973 DT denotes a
T16654 974-982 NNP denotes FACSAria
T16655 983-987 NN denotes flow
T16656 988-997 NN denotes cytometry
T16657 998-999 -LRB- denotes (
T16658 999-1001 NNP denotes BD
T16659 1002-1013 NNPS denotes Biosciences
T16660 1013-1014 -RRB- denotes )
T16661 1014-1015 , denotes ,
T16662 1016-1019 CC denotes and
T16663 1020-1023 DT denotes the
T16664 1024-1028 NNS denotes data
T16665 1029-1033 VBD denotes were
T16666 1034-1042 VBN denotes analyzed
T16667 1043-1048 VBG denotes using
T16668 1049-1052 DT denotes the
T16669 1053-1059 NNP denotes WinMDI
T16670 1060-1071 JJ denotes statistical
T16671 1072-1080 NN denotes software
T16672 1081-1082 -LRB- denotes (
T16673 1082-1089 NNP denotes Scripps
T16674 1089-1090 , denotes ,
T16675 1091-1093 NNP denotes La
T16676 1094-1099 NNP denotes Jolla
T16677 1099-1100 , denotes ,
T16678 1101-1103 NNP denotes CA
T16679 1103-1104 , denotes ,
T16680 1105-1108 NNP denotes USA
T16681 1108-1109 -RRB- denotes )
T16682 1109-1110 . denotes .
T16683 1111-1118 RB denotes Forward
T16684 1119-1122 CC denotes and
T16685 1123-1127 NN denotes side
T16686 1128-1135 NN denotes scatter
T16687 1136-1146 NNS denotes parameters
T16688 1147-1151 VBD denotes were
T16689 1152-1156 VBN denotes used
T16690 1157-1159 TO denotes to
T16691 1160-1164 NN denotes gate
T16692 1165-1167 IN denotes on
T16693 1168-1179 NNS denotes lymphocytes
T16694 1179-1180 . denotes .
R11816 T16470 T16472 nsubjpass Lymphocytes,obtained
R11817 T16471 T16472 auxpass were,obtained
R11818 T16472 T16472 ROOT obtained,obtained
R11819 T16473 T16472 prep from,obtained
R11820 T16474 T16477 nmod tumor,tissues
R11821 T16475 T16474 cc and,tumor
R11822 T16476 T16474 conj spleen,tumor
R11823 T16477 T16473 pobj tissues,from
R11824 T16478 T16477 prep for,tissues
R11825 T16479 T16480 compound phenotype,analysis
R11826 T16480 T16478 pobj analysis,for
R11827 T16481 T16472 punct .,obtained
R11828 T16482 T16486 nsubj Spleen,were
R11829 T16483 T16482 cc and,Spleen
R11830 T16484 T16485 compound tumor,lymphocytes
R11831 T16485 T16482 conj lymphocytes,Spleen
R11832 T16486 T16487 auxpass were,isolated
R11833 T16487 T16487 ROOT isolated,isolated
R11834 T16488 T16487 prep from,isolated
R11835 T16489 T16490 amod fresh,spleen
R11836 T16490 T16488 pobj spleen,from
R11837 T16491 T16490 cc and,spleen
R11838 T16492 T16493 compound tumor,biopsies
R11839 T16493 T16490 conj biopsies,spleen
R11840 T16494 T16487 conj obtained,isolated
R11841 T16495 T16494 prep from,obtained
R11842 T16496 T16497 nummod three,mice
R11843 T16497 T16495 pobj mice,from
R11844 T16498 T16497 prep per,mice
R11845 T16499 T16498 pobj group,per
R11846 T16500 T16487 punct .,isolated
R11847 T16501 T16507 nsubjpass Spleen,homogenized
R11848 T16502 T16501 cc and,Spleen
R11849 T16503 T16504 compound tumor,biopsies
R11850 T16504 T16501 conj biopsies,Spleen
R11851 T16505 T16507 auxpass were,homogenized
R11852 T16506 T16507 advmod briefly,homogenized
R11853 T16507 T16524 ccomp homogenized,placed
R11854 T16508 T16507 advmod mechanically,homogenized
R11855 T16509 T16507 prep in,homogenized
R11856 T16510 T16509 pobj PBS,in
R11857 T16511 T16507 punct ",",homogenized
R11858 T16512 T16507 conj filtered,homogenized
R11859 T16513 T16517 punct (,strainer
R11860 T16514 T16517 nummod 100,strainer
R11861 T16515 T16517 compound µm,strainer
R11862 T16516 T16517 compound cell,strainer
R11863 T16517 T16512 appos strainer,filtered
R11864 T16518 T16524 punct ;,placed
R11865 T16519 T16520 compound BD,PharMingen
R11866 T16520 T16524 nsubj PharMingen,placed
R11867 T16521 T16520 punct ),PharMingen
R11868 T16522 T16520 cc and,PharMingen
R11869 T16523 T16524 advmod then,placed
R11870 T16524 T16524 ROOT placed,placed
R11871 T16525 T16524 prep in,placed
R11872 T16526 T16527 compound lysis,buffer
R11873 T16527 T16525 pobj buffer,in
R11874 T16528 T16531 punct (,buffer
R11875 T16529 T16531 compound ACK,buffer
R11876 T16530 T16531 compound lysing,buffer
R11877 T16531 T16527 appos buffer,buffer
R11878 T16532 T16531 punct ",",buffer
R11879 T16533 T16531 appos LONZA,buffer
R11880 T16534 T16531 punct ),buffer
R11881 T16535 T16536 aux to,remove
R11882 T16536 T16524 advcl remove,placed
R11883 T16537 T16539 amod red,cells
R11884 T16538 T16539 compound blood,cells
R11885 T16539 T16536 dobj cells,remove
R11886 T16540 T16524 punct .,placed
R11887 T16541 T16542 compound One,million
R11888 T16542 T16543 nummod million,splenocytes
R11889 T16543 T16545 nsubjpass splenocytes,washed
R11890 T16544 T16545 auxpass were,washed
R11891 T16545 T16545 ROOT washed,washed
R11892 T16546 T16545 advmod once,washed
R11893 T16547 T16545 prep in,washed
R11894 T16548 T16547 pobj PBS,in
R11895 T16549 T16545 advcl containing,washed
R11896 T16550 T16551 nummod 1,%
R11897 T16551 T16554 compound %,albumin
R11898 T16552 T16553 amod bovine,serum
R11899 T16553 T16554 compound serum,albumin
R11900 T16554 T16549 dobj albumin,containing
R11901 T16555 T16556 punct (,BSA
R11902 T16556 T16554 appos BSA,albumin
R11903 T16557 T16556 punct ),BSA
R11904 T16558 T16554 cc and,albumin
R11905 T16559 T16554 conj re-suspended,albumin
R11906 T16560 T16559 prep in,re-suspended
R11907 T16561 T16562 nummod 100,µl
R11908 T16562 T16560 pobj µl,in
R11909 T16563 T16562 prep of,µl
R11910 T16564 T16565 compound PBS/BSA,buffer
R11911 T16565 T16563 pobj buffer,of
R11912 T16566 T16545 punct .,washed
R11913 T16567 T16577 advmod Meanwhile,placed
R11914 T16568 T16577 punct ",",placed
R11915 T16569 T16570 compound one,million
R11916 T16570 T16571 nummod million,cells
R11917 T16571 T16577 nsubjpass cells,placed
R11918 T16572 T16571 acl obtained,cells
R11919 T16573 T16572 prep from,obtained
R11920 T16574 T16575 compound tumor,tissues
R11921 T16575 T16573 pobj tissues,from
R11922 T16576 T16577 auxpass were,placed
R11923 T16577 T16577 ROOT placed,placed
R11924 T16578 T16577 prep in,placed
R11925 T16579 T16580 compound RPMI,medium
R11926 T16580 T16578 pobj medium,in
R11927 T16581 T16577 punct ",",placed
R11928 T16582 T16577 conj incubated,placed
R11929 T16583 T16584 aux to,obtain
R11930 T16584 T16582 advcl obtain,incubated
R11931 T16585 T16586 compound suspension,cell
R11932 T16586 T16584 dobj cell,obtain
R11933 T16587 T16586 prep for,cell
R11934 T16588 T16587 pobj 1hour,for
R11935 T16589 T16584 prep at,obtain
R11936 T16590 T16591 nummod 37,°
R11937 T16591 T16592 nummod °,C
R11938 T16592 T16589 pobj C,at
R11939 T16593 T16592 punct ",",C
R11940 T16594 T16592 amod centrifuged,C
R11941 T16595 T16594 punct (,centrifuged
R11942 T16596 T16597 nummod 1200,rpm
R11943 T16597 T16594 appos rpm,centrifuged
R11944 T16598 T16600 punct ",",min
R11945 T16599 T16600 nummod 3,min
R11946 T16600 T16597 appos min,rpm
R11947 T16601 T16597 punct ),rpm
R11948 T16602 T16594 cc and,centrifuged
R11949 T16603 T16604 advmod then,washed
R11950 T16604 T16594 conj washed,centrifuged
R11951 T16605 T16604 prep in,washed
R11952 T16606 T16605 pobj PBS,in
R11953 T16607 T16577 punct .,placed
R11954 T16608 T16613 advmod Next,incubated
R11955 T16609 T16613 punct ",",incubated
R11956 T16610 T16611 det the,lymphocytes
R11957 T16611 T16613 nsubjpass lymphocytes,incubated
R11958 T16612 T16613 auxpass were,incubated
R11959 T16613 T16613 ROOT incubated,incubated
R11960 T16614 T16613 prep with,incubated
R11961 T16615 T16618 amod various,antibodies
R11962 T16616 T16618 amod conjugated,antibodies
R11963 T16617 T16618 amod monoclonal,antibodies
R11964 T16618 T16614 pobj antibodies,with
R11965 T16619 T16618 punct ",",antibodies
R11966 T16620 T16618 prep including,antibodies
R11967 T16621 T16620 pobj CD4-APC,including
R11968 T16622 T16621 punct ",",CD4-APC
R11969 T16623 T16621 conj CD8-FITC,CD4-APC
R11970 T16624 T16623 cc and,CD8-FITC
R11971 T16625 T16623 conj CD19-PE,CD8-FITC
R11972 T16626 T16613 prep for,incubated
R11973 T16627 T16626 pobj 20min,for
R11974 T16628 T16613 prep at,incubated
R11975 T16629 T16630 nummod 4,°
R11976 T16630 T16631 nummod °,C
R11977 T16631 T16628 pobj C,at
R11978 T16632 T16633 punct ",",washed
R11979 T16633 T16613 dep washed,incubated
R11980 T16634 T16633 advmod twice,washed
R11981 T16635 T16633 prep in,washed
R11982 T16636 T16635 pobj PBS,in
R11983 T16637 T16633 punct ",",washed
R11984 T16638 T16633 cc and,washed
R11985 T16639 T16633 conj re-suspended,washed
R11986 T16640 T16639 prep in,re-suspended
R11987 T16641 T16642 nummod 400,µl
R11988 T16642 T16640 pobj µl,in
R11989 T16643 T16642 prep of,µl
R11990 T16644 T16643 pobj PBS,of
R11991 T16645 T16613 punct .,incubated
R11992 T16646 T16649 det A,analysis
R11993 T16647 T16649 nmod flow,analysis
R11994 T16648 T16649 amod cytometric,analysis
R11995 T16649 T16651 nsubjpass analysis,performed
R11996 T16650 T16651 auxpass was,performed
R11997 T16651 T16651 ROOT performed,performed
R11998 T16652 T16651 prep on,performed
R11999 T16653 T16655 det a,flow
R12000 T16654 T16655 compound FACSAria,flow
R12001 T16655 T16652 pobj flow,on
R12002 T16656 T16651 conj cytometry,performed
R12003 T16657 T16659 punct (,Biosciences
R12004 T16658 T16659 compound BD,Biosciences
R12005 T16659 T16656 appos Biosciences,cytometry
R12006 T16660 T16659 punct ),Biosciences
R12007 T16661 T16656 punct ",",cytometry
R12008 T16662 T16656 cc and,cytometry
R12009 T16663 T16664 det the,data
R12010 T16664 T16666 nsubjpass data,analyzed
R12011 T16665 T16666 auxpass were,analyzed
R12012 T16666 T16656 conj analyzed,cytometry
R12013 T16667 T16666 advcl using,analyzed
R12014 T16668 T16671 det the,software
R12015 T16669 T16671 nmod WinMDI,software
R12016 T16670 T16671 amod statistical,software
R12017 T16671 T16667 dobj software,using
R12018 T16672 T16681 punct (,)
R12019 T16673 T16681 nmod Scripps,)
R12020 T16674 T16673 punct ",",Scripps
R12021 T16675 T16676 compound La,Jolla
R12022 T16676 T16673 conj Jolla,Scripps
R12023 T16677 T16676 punct ",",Jolla
R12024 T16678 T16676 conj CA,Jolla
R12025 T16679 T16678 punct ",",CA
R12026 T16680 T16678 conj USA,CA
R12027 T16681 T16671 punct ),software
R12028 T16682 T16666 punct .,analyzed
R12029 T16683 T16687 amod Forward,parameters
R12030 T16684 T16683 cc and,Forward
R12031 T16685 T16687 compound side,parameters
R12032 T16686 T16687 compound scatter,parameters
R12033 T16687 T16689 nsubjpass parameters,used
R12034 T16688 T16689 auxpass were,used
R12035 T16689 T16689 ROOT used,used
R12036 T16690 T16691 aux to,gate
R12037 T16691 T16689 xcomp gate,used
R12038 T16692 T16691 prep on,gate
R12039 T16693 T16692 pobj lymphocytes,on
R12040 T16694 T16689 punct .,used