PMC:3317373 / 5456-7257 JSONTXT 9 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T2674 0-3 CD denotes 2.4
T2675 3-4 . denotes .
T2676 5-20 NNP denotes Electrophoretic
T2677 21-29 NNP denotes Mobility
T2678 30-35 NNP denotes Shift
T2679 36-41 NNP denotes Assay
T2680 42-43 -LRB- denotes (
T2681 43-47 NNP denotes EMSA
T2682 47-48 -RRB- denotes )
T2683 49-59 NNPS denotes Monolayers
T2684 60-62 IN denotes of
T2685 63-73 NNS denotes astrocytes
T2686 74-78 VBD denotes were
T2687 79-85 VBN denotes washed
T2688 86-90 IN denotes with
T2689 91-94 NNP denotes PBS
T2690 95-98 CC denotes and
T2691 99-108 VBN denotes harvested
T2692 109-111 IN denotes by
T2693 112-120 VBG denotes scraping
T2694 121-125 IN denotes into
T2695 126-130 JJ denotes cold
T2696 131-134 NNP denotes PBS
T2697 134-135 . denotes .
T2698 136-139 DT denotes The
T2699 140-144 NN denotes cell
T2700 145-151 NN denotes pellet
T2701 152-160 VBN denotes obtained
T2702 161-163 IN denotes by
T2703 164-178 NN denotes centrifugation
T2704 179-182 VBD denotes was
T2705 183-194 VBN denotes resuspended
T2706 195-197 IN denotes in
T2707 198-204 NN denotes buffer
T2708 205-215 VBG denotes containing
T2709 216-218 CD denotes 10
T2710 219-221 NNP denotes mM
T2711 222-227 NNP denotes HEPES
T2712 228-229 -LRB- denotes (
T2713 229-231 NNP denotes pH
T2714 232-235 CD denotes 7.9
T2715 235-236 -RRB- denotes )
T2716 236-237 , denotes ,
T2717 238-240 CD denotes 10
T2718 241-243 NNP denotes mM
T2719 244-247 NNP denotes KCl
T2720 247-248 , denotes ,
T2721 249-252 CD denotes 0.1
T2722 253-255 NNP denotes mM
T2723 256-260 NNP denotes EDTA
T2724 260-261 , denotes ,
T2725 262-265 CD denotes 0.1
T2726 266-268 NNP denotes mM
T2727 269-273 NNP denotes EGTA
T2728 273-274 , denotes ,
T2729 275-278 CD denotes 1.0
T2730 279-281 NNP denotes mM
T2731 282-285 NNP denotes DTT
T2732 285-286 , denotes ,
T2733 287-290 CC denotes and
T2734 291-294 CD denotes 0.5
T2735 295-297 NNP denotes mM
T2736 298-318 NN denotes phenylmethylsulfonyl
T2737 319-327 NN denotes fluoride
T2738 327-328 . denotes .
T2739 329-333 RB denotes Then
T2740 334-336 CD denotes 10
T2741 336-337 NN denotes %
T2742 338-345 NNP denotes Nonidet
T2743 346-350 NNP denotes P-40
T2744 351-354 VBD denotes was
T2745 355-360 VBN denotes added
T2746 361-364 CC denotes and
T2747 365-373 VBN denotes vortexed
T2748 374-381 NN denotes briefly
T2749 381-382 , denotes ,
T2750 383-386 CC denotes and
T2751 387-390 DT denotes the
T2752 391-397 NNS denotes nuclei
T2753 398-402 VBD denotes were
T2754 403-411 VBN denotes pelleted
T2755 412-414 IN denotes by
T2756 415-429 NN denotes centrifugation
T2757 429-430 . denotes .
T2758 431-434 DT denotes The
T2759 435-442 JJ denotes nuclear
T2760 443-451 NNS denotes proteins
T2761 452-456 VBD denotes were
T2762 457-466 VBN denotes extracted
T2763 467-471 IN denotes with
T2764 472-478 NN denotes buffer
T2765 479-489 VBG denotes containing
T2766 490-492 CD denotes 20
T2767 493-495 NNP denotes mM
T2768 496-501 NNP denotes HEPES
T2769 502-503 -LRB- denotes (
T2770 503-505 NNP denotes pH
T2771 506-509 CD denotes 7.9
T2772 509-510 -RRB- denotes )
T2773 510-511 , denotes ,
T2774 512-515 CD denotes 0.4
T2775 516-518 NNP denotes mM
T2776 519-523 NNP denotes NaCl
T2777 523-524 , denotes ,
T2778 525-528 CD denotes 1.0
T2779 529-531 NNP denotes mM
T2780 532-536 NNP denotes EDTA
T2781 536-537 , denotes ,
T2782 538-541 CD denotes 1.0
T2783 542-544 NNP denotes mM
T2784 545-549 NNP denotes EGTA
T2785 549-550 , denotes ,
T2786 551-554 CD denotes 1.0
T2787 555-557 NNP denotes mM
T2788 558-561 NNP denotes DTT
T2789 561-562 , denotes ,
T2790 563-566 CC denotes and
T2791 567-570 CD denotes 1.0
T2792 571-573 NNP denotes mM
T2793 574-594 NN denotes phenylmethylsulfonyl
T2794 595-603 NN denotes fluoride
T2795 603-604 . denotes .
T2796 605-614 NNP denotes Insoluble
T2797 615-623 NN denotes material
T2798 624-627 VBD denotes was
T2799 628-635 VBN denotes removed
T2800 636-638 IN denotes by
T2801 639-653 NN denotes centrifugation
T2802 654-656 IN denotes at
T2803 657-662 CD denotes 14000
T2804 663-666 NN denotes rpm
T2805 666-667 , denotes ,
T2806 668-671 CC denotes and
T2807 672-675 DT denotes the
T2808 676-687 JJ denotes supernatant
T2809 688-698 VBG denotes containing
T2810 699-702 DT denotes the
T2811 703-710 JJ denotes nuclear
T2812 711-719 NNS denotes proteins
T2813 720-723 VBD denotes was
T2814 724-730 VBN denotes stored
T2815 731-733 IN denotes at
T2816 735-737 CD denotes 80
T2817 737-738 CD denotes °
T2818 738-739 NNP denotes C
T2819 740-745 IN denotes until
T2820 746-749 NN denotes use
T2821 749-750 . denotes .
T2822 751-758 NN denotes Protein
T2823 759-772 NN denotes concentration
T2824 773-776 VBD denotes was
T2825 777-787 VBN denotes determined
T2826 788-793 VBG denotes using
T2827 794-795 DT denotes a
T2828 796-809 JJ denotes bicinchoninic
T2829 810-814 NN denotes acid
T2830 815-820 NN denotes assay
T2831 821-824 NN denotes kit
T2832 825-829 IN denotes with
T2833 830-836 JJ denotes bovine
T2834 837-842 NN denotes serum
T2835 843-850 NN denotes albumin
T2836 851-853 IN denotes as
T2837 854-857 DT denotes the
T2838 858-866 NN denotes standard
T2839 867-868 -LRB- denotes (
T2840 868-874 NNP denotes Pierce
T2841 875-887 NNP denotes Biochemicals
T2842 887-888 , denotes ,
T2843 889-897 NNP denotes Rockford
T2844 897-898 , denotes ,
T2845 899-901 NNP denotes IL
T2846 901-902 , denotes ,
T2847 903-906 NNP denotes USA
T2848 906-907 -RRB- denotes )
T2849 907-908 . denotes .
T2850 909-913 NNP denotes EMSA
T2851 914-917 VBD denotes was
T2852 918-927 VBN denotes performed
T2853 928-933 VBG denotes using
T2854 934-937 NN denotes gel
T2855 938-943 NN denotes shift
T2856 944-949 NN denotes assay
T2857 950-956 NN denotes system
T2858 957-958 -LRB- denotes (
T2859 958-965 NNP denotes Promega
T2860 965-966 , denotes ,
T2861 967-974 NNP denotes Madison
T2862 974-975 , denotes ,
T2863 976-978 NNP denotes WI
T2864 978-979 , denotes ,
T2865 980-983 NNP denotes USA
T2866 983-984 -RRB- denotes )
T2867 984-985 . denotes .
T2868 986-995 NNP denotes Consensus
T2869 996-1011 NN denotes oligonucleotide
T2870 1012-1017 NN denotes probe
T2871 1018-1019 -LRB- denotes (
T2872 1019-1020 CD denotes 5
T2873 1020-1021 SYM denotes
T2874 1021-1022 : denotes -
T2875 1022-1046 NN denotes AGTTGAGGGGACTTTCCCAGGG-3
T2876 1046-1047 NN denotes
T2877 1047-1048 -RRB- denotes )
T2878 1049-1052 VBD denotes was
T2879 1053-1064 VBN denotes end-labeled
T2880 1065-1067 IN denotes by
T2881 1068-1085 NN denotes T4-polynucleotide
T2882 1086-1092 NN denotes kinase
T2883 1093-1098 VBG denotes using
T2884 1099-1100 NNP denotes [
T2885 1100-1105 NNP denotes γ-32P
T2886 1105-1106 NNP denotes ]
T2887 1106-1107 : denotes -
T2888 1107-1110 NNP denotes ATP
T2889 1111-1112 -LRB- denotes (
T2890 1112-1116 NNP denotes Free
T2891 1117-1125 NNP denotes Biotech.
T2892 1125-1126 , denotes ,
T2893 1127-1134 NNP denotes Beijing
T2894 1134-1135 , denotes ,
T2895 1136-1141 NNP denotes China
T2896 1141-1142 -RRB- denotes )
T2897 1142-1143 . denotes .
T2898 1144-1151 NNP denotes Nuclear
T2899 1152-1159 NN denotes protein
T2900 1160-1161 -LRB- denotes (
T2901 1161-1163 CD denotes 20
T2902 1164-1166 NN denotes μg
T2903 1166-1167 -RRB- denotes )
T2904 1168-1171 VBD denotes was
T2905 1172-1184 VBN denotes preincubated
T2906 1185-1187 IN denotes in
T2907 1188-1190 CD denotes 20
T2908 1191-1193 NN denotes μL
T2909 1194-1201 JJ denotes binding
T2910 1202-1208 NN denotes buffer
T2911 1209-1219 VBG denotes containing
T2912 1220-1222 CD denotes 10
T2913 1223-1225 NNP denotes mM
T2914 1226-1234 NNP denotes Tris-HCl
T2915 1235-1236 -LRB- denotes (
T2916 1236-1238 NNP denotes PH
T2917 1239-1242 CD denotes 7.5
T2918 1242-1243 -RRB- denotes )
T2919 1243-1244 , denotes ,
T2920 1245-1246 CD denotes 1
T2921 1247-1249 NNP denotes mM
T2922 1250-1255 NNP denotes MgCl2
T2923 1255-1256 , denotes ,
T2924 1257-1260 CD denotes 0.5
T2925 1261-1263 NNP denotes mM
T2926 1264-1268 NNP denotes NaCl
T2927 1268-1269 , denotes ,
T2928 1270-1271 CD denotes 4
T2929 1271-1272 NN denotes %
T2930 1273-1281 NN denotes glycerol
T2931 1281-1282 , denotes ,
T2932 1283-1286 CD denotes 0.5
T2933 1287-1289 NNP denotes mM
T2934 1290-1294 NNP denotes EDTA
T2935 1294-1295 , denotes ,
T2936 1296-1299 CD denotes 0.5
T2937 1300-1302 NNP denotes mM
T2938 1303-1306 NNP denotes DTT
T2939 1306-1307 , denotes ,
T2940 1308-1311 CC denotes and
T2941 1312-1313 CD denotes 2
T2942 1314-1316 NN denotes μg
T2943 1317-1321 NN denotes poly
T2944 1322-1327 NN denotes dI-dC
T2945 1328-1331 IN denotes for
T2946 1332-1334 CD denotes 20
T2947 1335-1342 NNS denotes minutes
T2948 1343-1345 IN denotes on
T2949 1346-1349 NN denotes ice
T2950 1349-1350 . denotes .
T2951 1351-1356 IN denotes After
T2952 1357-1365 NN denotes addition
T2953 1366-1368 IN denotes of
T2954 1369-1372 DT denotes the
T2955 1373-1374 CD denotes 1
T2956 1375-1377 NNP denotes μL
T2957 1378-1388 JJ denotes 32P-labled
T2958 1389-1404 NN denotes oligonucleotide
T2959 1405-1410 NN denotes probe
T2960 1410-1411 , denotes ,
T2961 1412-1415 DT denotes the
T2962 1416-1426 NN denotes incubation
T2963 1427-1430 VBD denotes was
T2964 1431-1440 VBN denotes continued
T2965 1441-1444 IN denotes for
T2966 1445-1447 CD denotes 20
T2967 1448-1455 NNS denotes minutes
T2968 1456-1458 IN denotes on
T2969 1459-1462 NN denotes ice
T2970 1462-1463 . denotes .
T2971 1464-1467 DT denotes The
T2972 1468-1479 JJ denotes DNA-protein
T2973 1480-1489 NNS denotes complexes
T2974 1490-1494 VBD denotes were
T2975 1495-1504 VBN denotes separated
T2976 1505-1507 IN denotes by
T2977 1508-1523 NN denotes electrophoresis
T2978 1524-1526 IN denotes on
T2979 1527-1528 CD denotes 4
T2980 1528-1529 NN denotes %
T2981 1530-1543 JJ denotes nondenaturing
T2982 1544-1558 NN denotes polyacrylamide
T2983 1559-1562 NN denotes gel
T2984 1563-1565 IN denotes in
T2985 1566-1569 CD denotes 0.5
T2986 1570-1571 CD denotes ×
T2987 1572-1575 NNP denotes TBE
T2988 1576-1582 NN denotes buffer
T2989 1583-1584 -LRB- denotes (
T2990 1584-1600 NNP denotes tris-borate-EDTA
T2991 1600-1601 -RRB- denotes )
T2992 1602-1604 IN denotes at
T2993 1605-1608 CD denotes 390
T2994 1609-1610 NN denotes V
T2995 1611-1614 IN denotes for
T2996 1615-1616 CD denotes 1
T2997 1617-1621 NN denotes hour
T2998 1622-1624 IN denotes at
T2999 1625-1626 CD denotes 4
T3000 1626-1627 CD denotes °
T3001 1627-1628 NNP denotes C
T3002 1628-1629 . denotes .
T3003 1630-1635 IN denotes After
T3004 1636-1651 NN denotes electrophoresis
T3005 1651-1652 , denotes ,
T3006 1653-1656 DT denotes the
T3007 1657-1660 NN denotes gel
T3008 1661-1664 VBD denotes was
T3009 1665-1670 VBN denotes dried
T3010 1671-1674 CC denotes and
T3011 1675-1682 VBN denotes exposed
T3012 1683-1685 TO denotes to
T3013 1686-1691 NN denotes X-ray
T3014 1692-1696 NN denotes film
T3015 1697-1698 -LRB- denotes (
T3016 1698-1702 NNP denotes Fuji
T3017 1703-1712 NNP denotes Hyperfilm
T3018 1712-1713 , denotes ,
T3019 1714-1719 NNP denotes Tokyo
T3020 1719-1720 , denotes ,
T3021 1721-1726 NNP denotes Japan
T3022 1726-1727 -RRB- denotes )
T3023 1727-1728 . denotes .
T3024 1729-1735 NNS denotes Levels
T3025 1736-1738 IN denotes of
T3026 1739-1744 NNP denotes NF-κB
T3027 1745-1748 NNP denotes DNA
T3028 1749-1756 JJ denotes binding
T3029 1757-1765 NN denotes activity
T3030 1766-1770 VBD denotes were
T3031 1771-1781 VBN denotes quantified
T3032 1782-1784 IN denotes by
T3033 1785-1793 NN denotes software
T3034 1794-1800 NNP denotes ImageJ
T3035 1800-1801 . denotes .
R2313 T2674 T2674 ROOT 2.4,2.4
R2314 T2675 T2674 punct .,2.4
R2315 T2676 T2679 compound Electrophoretic,Assay
R2316 T2677 T2678 compound Mobility,Shift
R2317 T2678 T2679 compound Shift,Assay
R2318 T2679 T2687 nsubjpass Assay,washed
R2319 T2680 T2681 punct (,EMSA
R2320 T2681 T2679 appos EMSA,Assay
R2321 T2682 T2681 punct ),EMSA
R2322 T2683 T2679 appos Monolayers,Assay
R2323 T2684 T2683 prep of,Monolayers
R2324 T2685 T2684 pobj astrocytes,of
R2325 T2686 T2687 auxpass were,washed
R2326 T2687 T2687 ROOT washed,washed
R2327 T2688 T2687 prep with,washed
R2328 T2689 T2688 pobj PBS,with
R2329 T2690 T2687 cc and,washed
R2330 T2691 T2687 conj harvested,washed
R2331 T2692 T2691 prep by,harvested
R2332 T2693 T2692 pcomp scraping,by
R2333 T2694 T2693 prep into,scraping
R2334 T2695 T2696 amod cold,PBS
R2335 T2696 T2694 pobj PBS,into
R2336 T2697 T2687 punct .,washed
R2337 T2698 T2700 det The,pellet
R2338 T2699 T2700 compound cell,pellet
R2339 T2700 T2704 nsubj pellet,was
R2340 T2701 T2700 acl obtained,pellet
R2341 T2702 T2701 agent by,obtained
R2342 T2703 T2702 pobj centrifugation,by
R2343 T2704 T2705 auxpass was,resuspended
R2344 T2705 T2705 ROOT resuspended,resuspended
R2345 T2706 T2705 prep in,resuspended
R2346 T2707 T2706 pobj buffer,in
R2347 T2708 T2707 acl containing,buffer
R2348 T2709 T2711 nummod 10,HEPES
R2349 T2710 T2711 compound mM,HEPES
R2350 T2711 T2708 dobj HEPES,containing
R2351 T2712 T2713 punct (,pH
R2352 T2713 T2711 appos pH,HEPES
R2353 T2714 T2713 nummod 7.9,pH
R2354 T2715 T2713 punct ),pH
R2355 T2716 T2711 punct ",",HEPES
R2356 T2717 T2719 nummod 10,KCl
R2357 T2718 T2719 compound mM,KCl
R2358 T2719 T2711 conj KCl,HEPES
R2359 T2720 T2719 punct ",",KCl
R2360 T2721 T2723 compound 0.1,EDTA
R2361 T2722 T2723 compound mM,EDTA
R2362 T2723 T2719 conj EDTA,KCl
R2363 T2724 T2723 punct ",",EDTA
R2364 T2725 T2727 nummod 0.1,EGTA
R2365 T2726 T2727 compound mM,EGTA
R2366 T2727 T2723 conj EGTA,EDTA
R2367 T2728 T2727 punct ",",EGTA
R2368 T2729 T2731 nummod 1.0,DTT
R2369 T2730 T2731 compound mM,DTT
R2370 T2731 T2727 conj DTT,EGTA
R2371 T2732 T2731 punct ",",DTT
R2372 T2733 T2731 cc and,DTT
R2373 T2734 T2737 nummod 0.5,fluoride
R2374 T2735 T2737 nmod mM,fluoride
R2375 T2736 T2737 compound phenylmethylsulfonyl,fluoride
R2376 T2737 T2731 conj fluoride,DTT
R2377 T2738 T2705 punct .,resuspended
R2378 T2739 T2745 advmod Then,added
R2379 T2740 T2741 nummod 10,%
R2380 T2741 T2743 compound %,P-40
R2381 T2742 T2743 compound Nonidet,P-40
R2382 T2743 T2745 nsubjpass P-40,added
R2383 T2744 T2745 auxpass was,added
R2384 T2745 T2745 ROOT added,added
R2385 T2746 T2745 cc and,added
R2386 T2747 T2745 conj vortexed,added
R2387 T2748 T2747 advmod briefly,vortexed
R2388 T2749 T2747 punct ",",vortexed
R2389 T2750 T2747 cc and,vortexed
R2390 T2751 T2752 det the,nuclei
R2391 T2752 T2754 nsubjpass nuclei,pelleted
R2392 T2753 T2754 auxpass were,pelleted
R2393 T2754 T2747 conj pelleted,vortexed
R2394 T2755 T2754 agent by,pelleted
R2395 T2756 T2755 pobj centrifugation,by
R2396 T2757 T2754 punct .,pelleted
R2397 T2758 T2760 det The,proteins
R2398 T2759 T2760 amod nuclear,proteins
R2399 T2760 T2762 nsubjpass proteins,extracted
R2400 T2761 T2762 auxpass were,extracted
R2401 T2762 T2762 ROOT extracted,extracted
R2402 T2763 T2762 prep with,extracted
R2403 T2764 T2763 pobj buffer,with
R2404 T2765 T2764 acl containing,buffer
R2405 T2766 T2768 nummod 20,HEPES
R2406 T2767 T2768 compound mM,HEPES
R2407 T2768 T2765 dobj HEPES,containing
R2408 T2769 T2770 punct (,pH
R2409 T2770 T2768 appos pH,HEPES
R2410 T2771 T2770 nummod 7.9,pH
R2411 T2772 T2770 punct ),pH
R2412 T2773 T2768 punct ",",HEPES
R2413 T2774 T2776 nummod 0.4,NaCl
R2414 T2775 T2776 compound mM,NaCl
R2415 T2776 T2768 appos NaCl,HEPES
R2416 T2777 T2776 punct ",",NaCl
R2417 T2778 T2780 nummod 1.0,EDTA
R2418 T2779 T2780 compound mM,EDTA
R2419 T2780 T2776 conj EDTA,NaCl
R2420 T2781 T2780 punct ",",EDTA
R2421 T2782 T2784 nummod 1.0,EGTA
R2422 T2783 T2784 compound mM,EGTA
R2423 T2784 T2780 conj EGTA,EDTA
R2424 T2785 T2784 punct ",",EGTA
R2425 T2786 T2788 nummod 1.0,DTT
R2426 T2787 T2788 compound mM,DTT
R2427 T2788 T2784 conj DTT,EGTA
R2428 T2789 T2788 punct ",",DTT
R2429 T2790 T2788 cc and,DTT
R2430 T2791 T2794 nummod 1.0,fluoride
R2431 T2792 T2794 compound mM,fluoride
R2432 T2793 T2794 compound phenylmethylsulfonyl,fluoride
R2433 T2794 T2788 conj fluoride,DTT
R2434 T2795 T2762 punct .,extracted
R2435 T2796 T2797 amod Insoluble,material
R2436 T2797 T2799 nsubjpass material,removed
R2437 T2798 T2799 auxpass was,removed
R2438 T2799 T2799 ROOT removed,removed
R2439 T2800 T2799 agent by,removed
R2440 T2801 T2800 pobj centrifugation,by
R2441 T2802 T2799 prep at,removed
R2442 T2803 T2804 nummod 14000,rpm
R2443 T2804 T2802 pobj rpm,at
R2444 T2805 T2799 punct ",",removed
R2445 T2806 T2799 cc and,removed
R2446 T2807 T2808 det the,supernatant
R2447 T2808 T2814 nsubjpass supernatant,stored
R2448 T2809 T2808 acl containing,supernatant
R2449 T2810 T2812 det the,proteins
R2450 T2811 T2812 amod nuclear,proteins
R2451 T2812 T2809 dobj proteins,containing
R2452 T2813 T2814 auxpass was,stored
R2453 T2814 T2799 conj stored,removed
R2454 T2815 T2814 prep at,stored
R2455 T2816 T2818 nummod 80,C
R2456 T2817 T2818 nummod °,C
R2457 T2818 T2815 pobj C,at
R2458 T2819 T2814 prep until,stored
R2459 T2820 T2819 pobj use,until
R2460 T2821 T2814 punct .,stored
R2461 T2822 T2823 compound Protein,concentration
R2462 T2823 T2825 nsubjpass concentration,determined
R2463 T2824 T2825 auxpass was,determined
R2464 T2825 T2825 ROOT determined,determined
R2465 T2826 T2825 advcl using,determined
R2466 T2827 T2831 det a,kit
R2467 T2828 T2831 amod bicinchoninic,kit
R2468 T2829 T2830 compound acid,assay
R2469 T2830 T2831 compound assay,kit
R2470 T2831 T2826 dobj kit,using
R2471 T2832 T2831 prep with,kit
R2472 T2833 T2834 amod bovine,serum
R2473 T2834 T2832 pobj serum,with
R2474 T2835 T2831 amod albumin,kit
R2475 T2836 T2826 prep as,using
R2476 T2837 T2838 det the,standard
R2477 T2838 T2836 pobj standard,as
R2478 T2839 T2841 punct (,Biochemicals
R2479 T2840 T2841 compound Pierce,Biochemicals
R2480 T2841 T2826 parataxis Biochemicals,using
R2481 T2842 T2841 punct ",",Biochemicals
R2482 T2843 T2841 npadvmod Rockford,Biochemicals
R2483 T2844 T2843 punct ",",Rockford
R2484 T2845 T2843 conj IL,Rockford
R2485 T2846 T2845 punct ",",IL
R2486 T2847 T2843 npadvmod USA,Rockford
R2487 T2848 T2843 punct ),Rockford
R2488 T2849 T2841 punct .,Biochemicals
R2489 T2850 T2852 nsubjpass EMSA,performed
R2490 T2851 T2852 auxpass was,performed
R2491 T2852 T2852 ROOT performed,performed
R2492 T2853 T2852 advcl using,performed
R2493 T2854 T2855 compound gel,shift
R2494 T2855 T2857 compound shift,system
R2495 T2856 T2857 compound assay,system
R2496 T2857 T2853 dobj system,using
R2497 T2858 T2857 punct (,system
R2498 T2859 T2857 appos Promega,system
R2499 T2860 T2859 punct ",",Promega
R2500 T2861 T2859 conj Madison,Promega
R2501 T2862 T2861 punct ",",Madison
R2502 T2863 T2861 conj WI,Madison
R2503 T2864 T2863 punct ",",WI
R2504 T2865 T2863 conj USA,WI
R2505 T2866 T2857 punct ),system
R2506 T2867 T2852 punct .,performed
R2507 T2868 T2870 compound Consensus,probe
R2508 T2869 T2870 compound oligonucleotide,probe
R2509 T2870 T2879 nsubjpass probe,end-labeled
R2510 T2871 T2870 punct (,probe
R2511 T2872 T2875 nummod 5,AGTTGAGGGGACTTTCCCAGGG-3
R2512 T2873 T2875 punct ′,AGTTGAGGGGACTTTCCCAGGG-3
R2513 T2874 T2875 punct -,AGTTGAGGGGACTTTCCCAGGG-3
R2514 T2875 T2870 appos AGTTGAGGGGACTTTCCCAGGG-3,probe
R2515 T2876 T2875 appos ′,AGTTGAGGGGACTTTCCCAGGG-3
R2516 T2877 T2875 punct ),AGTTGAGGGGACTTTCCCAGGG-3
R2517 T2878 T2879 auxpass was,end-labeled
R2518 T2879 T2879 ROOT end-labeled,end-labeled
R2519 T2880 T2879 agent by,end-labeled
R2520 T2881 T2882 compound T4-polynucleotide,kinase
R2521 T2882 T2880 pobj kinase,by
R2522 T2883 T2879 advcl using,end-labeled
R2523 T2884 T2888 compound [,ATP
R2524 T2885 T2886 compound γ-32P,]
R2525 T2886 T2888 compound ],ATP
R2526 T2887 T2888 punct -,ATP
R2527 T2888 T2883 dobj ATP,using
R2528 T2889 T2891 punct (,Biotech.
R2529 T2890 T2891 compound Free,Biotech.
R2530 T2891 T2888 appos Biotech.,ATP
R2531 T2892 T2891 punct ",",Biotech.
R2532 T2893 T2891 npadvmod Beijing,Biotech.
R2533 T2894 T2893 punct ",",Beijing
R2534 T2895 T2891 npadvmod China,Biotech.
R2535 T2896 T2891 punct ),Biotech.
R2536 T2897 T2879 punct .,end-labeled
R2537 T2898 T2899 compound Nuclear,protein
R2538 T2899 T2904 nsubj protein,was
R2539 T2900 T2899 punct (,protein
R2540 T2901 T2902 nummod 20,μg
R2541 T2902 T2899 appos μg,protein
R2542 T2903 T2899 punct ),protein
R2543 T2904 T2905 auxpass was,preincubated
R2544 T2905 T2905 ROOT preincubated,preincubated
R2545 T2906 T2905 prep in,preincubated
R2546 T2907 T2908 nummod 20,μL
R2547 T2908 T2910 nmod μL,buffer
R2548 T2909 T2910 amod binding,buffer
R2549 T2910 T2906 pobj buffer,in
R2550 T2911 T2910 acl containing,buffer
R2551 T2912 T2914 nummod 10,Tris-HCl
R2552 T2913 T2914 compound mM,Tris-HCl
R2553 T2914 T2911 dobj Tris-HCl,containing
R2554 T2915 T2916 punct (,PH
R2555 T2916 T2914 appos PH,Tris-HCl
R2556 T2917 T2916 nummod 7.5,PH
R2557 T2918 T2916 punct ),PH
R2558 T2919 T2914 punct ",",Tris-HCl
R2559 T2920 T2922 nummod 1,MgCl2
R2560 T2921 T2922 compound mM,MgCl2
R2561 T2922 T2914 conj MgCl2,Tris-HCl
R2562 T2923 T2922 punct ",",MgCl2
R2563 T2924 T2922 npadvmod 0.5,MgCl2
R2564 T2925 T2926 compound mM,NaCl
R2565 T2926 T2926 ROOT NaCl,NaCl
R2566 T2927 T2926 punct ",",NaCl
R2567 T2928 T2929 nummod 4,%
R2568 T2929 T2930 compound %,glycerol
R2569 T2930 T2926 appos glycerol,NaCl
R2570 T2931 T2930 punct ",",glycerol
R2571 T2932 T2934 nummod 0.5,EDTA
R2572 T2933 T2934 compound mM,EDTA
R2573 T2934 T2926 conj EDTA,NaCl
R2574 T2935 T2934 punct ",",EDTA
R2575 T2936 T2938 nummod 0.5,DTT
R2576 T2937 T2938 compound mM,DTT
R2577 T2938 T2934 conj DTT,EDTA
R2578 T2939 T2938 punct ",",DTT
R2579 T2940 T2938 cc and,DTT
R2580 T2941 T2942 nummod 2,μg
R2581 T2942 T2944 compound μg,dI-dC
R2582 T2943 T2944 compound poly,dI-dC
R2583 T2944 T2934 appos dI-dC,EDTA
R2584 T2945 T2944 prep for,dI-dC
R2585 T2946 T2947 nummod 20,minutes
R2586 T2947 T2945 pobj minutes,for
R2587 T2948 T2947 prep on,minutes
R2588 T2949 T2948 pobj ice,on
R2589 T2950 T2926 punct .,NaCl
R2590 T2951 T2964 prep After,continued
R2591 T2952 T2951 pobj addition,After
R2592 T2953 T2952 prep of,addition
R2593 T2954 T2959 det the,probe
R2594 T2955 T2956 nummod 1,μL
R2595 T2956 T2959 nmod μL,probe
R2596 T2957 T2959 amod 32P-labled,probe
R2597 T2958 T2959 compound oligonucleotide,probe
R2598 T2959 T2953 pobj probe,of
R2599 T2960 T2964 punct ",",continued
R2600 T2961 T2962 det the,incubation
R2601 T2962 T2964 nsubjpass incubation,continued
R2602 T2963 T2964 auxpass was,continued
R2603 T2964 T2964 ROOT continued,continued
R2604 T2965 T2964 prep for,continued
R2605 T2966 T2967 nummod 20,minutes
R2606 T2967 T2965 pobj minutes,for
R2607 T2968 T2967 prep on,minutes
R2608 T2969 T2968 pobj ice,on
R2609 T2970 T2964 punct .,continued
R2610 T2971 T2973 det The,complexes
R2611 T2972 T2973 amod DNA-protein,complexes
R2612 T2973 T2975 nsubjpass complexes,separated
R2613 T2974 T2975 auxpass were,separated
R2614 T2975 T2975 ROOT separated,separated
R2615 T2976 T2975 agent by,separated
R2616 T2977 T2976 pobj electrophoresis,by
R2617 T2978 T2975 prep on,separated
R2618 T2979 T2980 nummod 4,%
R2619 T2980 T2983 nmod %,gel
R2620 T2981 T2983 amod nondenaturing,gel
R2621 T2982 T2983 compound polyacrylamide,gel
R2622 T2983 T2978 pobj gel,on
R2623 T2984 T2975 prep in,separated
R2624 T2985 T2988 nummod 0.5,buffer
R2625 T2986 T2988 nummod ×,buffer
R2626 T2987 T2988 compound TBE,buffer
R2627 T2988 T2984 pobj buffer,in
R2628 T2989 T2988 punct (,buffer
R2629 T2990 T2988 appos tris-borate-EDTA,buffer
R2630 T2991 T2988 punct ),buffer
R2631 T2992 T2975 prep at,separated
R2632 T2993 T2994 nummod 390,V
R2633 T2994 T2992 pobj V,at
R2634 T2995 T2975 prep for,separated
R2635 T2996 T2997 nummod 1,hour
R2636 T2997 T2995 pobj hour,for
R2637 T2998 T2975 prep at,separated
R2638 T2999 T3000 nummod 4,°
R2639 T3000 T2998 pobj °,at
R2640 T3001 T2975 npadvmod C,separated
R2641 T3002 T2975 punct .,separated
R2642 T3003 T3009 prep After,dried
R2643 T3004 T3003 pobj electrophoresis,After
R2644 T3005 T3009 punct ",",dried
R2645 T3006 T3007 det the,gel
R2646 T3007 T3009 nsubjpass gel,dried
R2647 T3008 T3009 auxpass was,dried
R2648 T3009 T3009 ROOT dried,dried
R2649 T3010 T3009 cc and,dried
R2650 T3011 T3009 conj exposed,dried
R2651 T3012 T3011 prep to,exposed
R2652 T3013 T3014 compound X-ray,film
R2653 T3014 T3012 pobj film,to
R2654 T3015 T3017 punct (,Hyperfilm
R2655 T3016 T3017 compound Fuji,Hyperfilm
R2656 T3017 T3014 appos Hyperfilm,film
R2657 T3018 T3017 punct ",",Hyperfilm
R2658 T3019 T3017 npadvmod Tokyo,Hyperfilm
R2659 T3020 T3019 punct ",",Tokyo
R2660 T3021 T3019 conj Japan,Tokyo
R2661 T3022 T3017 punct ),Hyperfilm
R2662 T3023 T3009 punct .,dried
R2663 T3024 T3031 nsubjpass Levels,quantified
R2664 T3025 T3024 prep of,Levels
R2665 T3026 T3027 compound NF-κB,DNA
R2666 T3027 T3025 pobj DNA,of
R2667 T3028 T3029 amod binding,activity
R2668 T3029 T3025 pobj activity,of
R2669 T3030 T3031 auxpass were,quantified
R2670 T3031 T3031 ROOT quantified,quantified
R2671 T3032 T3031 agent by,quantified
R2672 T3033 T3034 compound software,ImageJ
R2673 T3034 T3032 pobj ImageJ,by
R2674 T3035 T3031 punct .,quantified