PMC:3279418 / 31854-32537 12 Projects
Annnotations
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T12090 | 0-18 | NNP | denotes | Immunofluorescence |
| T12091 | 19-29 | NNP | denotes | Fibroblast |
| T12092 | 30-33 | CC | denotes | and |
| T12093 | 34-44 | JJ | denotes | macrophage |
| T12094 | 45-50 | NNS | denotes | cells |
| T12095 | 51-55 | VBD | denotes | were |
| T12096 | 56-67 | VBN | denotes | transfected |
| T12097 | 68-72 | IN | denotes | with |
| T12098 | 73-86 | NNP | denotes | pFLAG-SHARPIN |
| T12099 | 86-87 | . | denotes | . |
| T12100 | 88-90 | CD | denotes | 48 |
| T12101 | 91-96 | NNS | denotes | hours |
| T12102 | 97-102 | RB | denotes | later |
| T12103 | 102-103 | , | denotes | , |
| T12104 | 104-109 | NNS | denotes | cells |
| T12105 | 110-114 | VBD | denotes | were |
| T12106 | 115-121 | VBN | denotes | washed |
| T12107 | 122-129 | RB | denotes | briefly |
| T12108 | 130-134 | IN | denotes | with |
| T12109 | 135-138 | NNP | denotes | PBS |
| T12110 | 138-139 | , | denotes | , |
| T12111 | 140-144 | RB | denotes | then |
| T12112 | 145-150 | VBN | denotes | fixed |
| T12113 | 151-153 | IN | denotes | in |
| T12114 | 154-158 | JJ | denotes | cold |
| T12115 | 159-167 | NN | denotes | methanol |
| T12116 | 168-170 | IN | denotes | at |
| T12117 | 171-172 | CD | denotes | − |
| T12118 | 172-174 | CD | denotes | 20 |
| T12119 | 174-175 | NN | denotes | ° |
| T12120 | 175-176 | NNP | denotes | C |
| T12121 | 177-180 | IN | denotes | for |
| T12122 | 181-183 | CD | denotes | 10 |
| T12123 | 184-191 | NNS | denotes | minutes |
| T12124 | 192-195 | CC | denotes | and |
| T12125 | 196-200 | JJ | denotes | cold |
| T12126 | 201-208 | NN | denotes | acetone |
| T12127 | 209-211 | IN | denotes | at |
| T12128 | 212-215 | CD | denotes | -20 |
| T12129 | 215-216 | CD | denotes | ° |
| T12130 | 216-217 | NNP | denotes | C |
| T12131 | 218-221 | IN | denotes | for |
| T12132 | 222-223 | CD | denotes | 1 |
| T12133 | 224-230 | NN | denotes | minute |
| T12134 | 230-231 | . | denotes | . |
| T12135 | 232-240 | NNP | denotes | Incubate |
| T12136 | 241-246 | NNS | denotes | cells |
| T12137 | 247-251 | IN | denotes | with |
| T12138 | 252-255 | NNP | denotes | PBS |
| T12139 | 255-257 | CD | denotes | +1 |
| T12140 | 257-258 | NN | denotes | % |
| T12141 | 259-262 | NNP | denotes | BSA |
| T12142 | 263-266 | IN | denotes | for |
| T12143 | 267-269 | CD | denotes | 15 |
| T12144 | 270-277 | NNS | denotes | minutes |
| T12145 | 278-280 | TO | denotes | to |
| T12146 | 281-286 | VB | denotes | block |
| T12147 | 287-299 | JJ | denotes | non-specific |
| T12148 | 300-307 | JJ | denotes | binding |
| T12149 | 307-308 | . | denotes | . |
| T12150 | 309-314 | VBN | denotes | Fixed |
| T12151 | 315-320 | NNS | denotes | cells |
| T12152 | 321-325 | VBD | denotes | were |
| T12153 | 326-330 | RB | denotes | then |
| T12154 | 331-340 | VBN | denotes | incubated |
| T12155 | 341-345 | IN | denotes | with |
| T12156 | 346-355 | JJ | denotes | anti-FLAG |
| T12157 | 356-357 | -LRB- | denotes | ( |
| T12158 | 357-358 | CD | denotes | 1 |
| T12159 | 358-359 | CD | denotes | ∶ |
| T12160 | 359-362 | CD | denotes | 100 |
| T12161 | 362-363 | -RRB- | denotes | ) |
| T12162 | 364-366 | IN | denotes | as |
| T12163 | 367-370 | DT | denotes | the |
| T12164 | 371-378 | JJ | denotes | primary |
| T12165 | 379-387 | NN | denotes | antibody |
| T12166 | 388-390 | IN | denotes | at |
| T12167 | 391-395 | NN | denotes | room |
| T12168 | 396-407 | NN | denotes | temperature |
| T12169 | 408-411 | IN | denotes | for |
| T12170 | 412-413 | CD | denotes | 1 |
| T12171 | 414-418 | NN | denotes | hour |
| T12172 | 418-419 | , | denotes | , |
| T12173 | 420-428 | VBN | denotes | followed |
| T12174 | 429-431 | IN | denotes | by |
| T12175 | 432-437 | CD | denotes | three |
| T12176 | 438-446 | JJ | denotes | 5-minute |
| T12177 | 447-453 | NNS | denotes | washes |
| T12178 | 453-454 | . | denotes | . |
| T12179 | 455-460 | NNS | denotes | Cells |
| T12180 | 461-465 | VBD | denotes | were |
| T12181 | 466-473 | JJ | denotes | further |
| T12182 | 474-483 | VBN | denotes | incubated |
| T12183 | 484-488 | IN | denotes | with |
| T12184 | 489-493 | JJ | denotes | goat |
| T12185 | 494-505 | JJ | denotes | anti-rabbit |
| T12186 | 506-514 | NN | denotes | IgG-FITC |
| T12187 | 515-516 | -LRB- | denotes | ( |
| T12188 | 516-517 | CD | denotes | 1 |
| T12189 | 517-518 | CD | denotes | ∶ |
| T12190 | 518-521 | CD | denotes | 100 |
| T12191 | 521-522 | -RRB- | denotes | ) |
| T12192 | 523-525 | IN | denotes | as |
| T12193 | 526-529 | DT | denotes | the |
| T12194 | 530-539 | JJ | denotes | secondary |
| T12195 | 540-548 | NN | denotes | antibody |
| T12196 | 549-551 | IN | denotes | at |
| T12197 | 552-556 | NN | denotes | room |
| T12198 | 557-568 | NN | denotes | temperature |
| T12199 | 569-572 | IN | denotes | for |
| T12200 | 573-575 | CD | denotes | 30 |
| T12201 | 576-583 | NNS | denotes | minutes |
| T12202 | 583-584 | , | denotes | , |
| T12203 | 585-593 | VBN | denotes | followed |
| T12204 | 594-596 | IN | denotes | by |
| T12205 | 597-602 | CD | denotes | three |
| T12206 | 603-611 | JJ | denotes | 5-minute |
| T12207 | 612-618 | NNS | denotes | washes |
| T12208 | 618-619 | . | denotes | . |
| T12209 | 620-625 | NNS | denotes | Cells |
| T12210 | 626-630 | VBD | denotes | were |
| T12211 | 631-635 | RB | denotes | then |
| T12212 | 636-644 | VBN | denotes | examined |
| T12213 | 645-650 | VBG | denotes | using |
| T12214 | 651-658 | JJ | denotes | inverse |
| T12215 | 659-671 | NN | denotes | fluorescence |
| T12216 | 672-682 | NN | denotes | microscope |
| T12217 | 682-683 | . | denotes | . |
| R10033 | T12090 | T12091 | compound | Immunofluorescence,Fibroblast |
| R10034 | T12091 | T12096 | nsubjpass | Fibroblast,transfected |
| R10035 | T12092 | T12091 | cc | and,Fibroblast |
| R10036 | T12093 | T12094 | amod | macrophage,cells |
| R10037 | T12094 | T12091 | conj | cells,Fibroblast |
| R10038 | T12095 | T12096 | auxpass | were,transfected |
| R10039 | T12096 | T12096 | ROOT | transfected,transfected |
| R10040 | T12097 | T12096 | prep | with,transfected |
| R10041 | T12098 | T12097 | pobj | pFLAG-SHARPIN,with |
| R10042 | T12099 | T12096 | punct | .,transfected |
| R10043 | T12100 | T12101 | nummod | 48,hours |
| R10044 | T12101 | T12102 | npadvmod | hours,later |
| R10045 | T12102 | T12106 | advmod | later,washed |
| R10046 | T12103 | T12106 | punct | ",",washed |
| R10047 | T12104 | T12106 | nsubjpass | cells,washed |
| R10048 | T12105 | T12106 | auxpass | were,washed |
| R10049 | T12106 | T12106 | ROOT | washed,washed |
| R10050 | T12107 | T12106 | advmod | briefly,washed |
| R10051 | T12108 | T12106 | prep | with,washed |
| R10052 | T12109 | T12108 | pobj | PBS,with |
| R10053 | T12110 | T12106 | punct | ",",washed |
| R10054 | T12111 | T12112 | advmod | then,fixed |
| R10055 | T12112 | T12106 | conj | fixed,washed |
| R10056 | T12113 | T12112 | prep | in,fixed |
| R10057 | T12114 | T12115 | amod | cold,methanol |
| R10058 | T12115 | T12113 | pobj | methanol,in |
| R10059 | T12116 | T12112 | prep | at,fixed |
| R10060 | T12117 | T12127 | preconj | −,at |
| R10061 | T12118 | T12119 | nummod | 20,° |
| R10062 | T12119 | T12120 | compound | °,C |
| R10063 | T12120 | T12117 | appos | C,− |
| R10064 | T12121 | T12120 | prep | for,C |
| R10065 | T12122 | T12123 | nummod | 10,minutes |
| R10066 | T12123 | T12121 | pobj | minutes,for |
| R10067 | T12124 | T12123 | cc | and,minutes |
| R10068 | T12125 | T12126 | amod | cold,acetone |
| R10069 | T12126 | T12123 | conj | acetone,minutes |
| R10070 | T12127 | T12112 | prep | at,fixed |
| R10071 | T12128 | T12129 | nummod | -20,° |
| R10072 | T12129 | T12130 | nummod | °,C |
| R10073 | T12130 | T12127 | pobj | C,at |
| R10074 | T12131 | T12112 | prep | for,fixed |
| R10075 | T12132 | T12133 | nummod | 1,minute |
| R10076 | T12133 | T12131 | pobj | minute,for |
| R10077 | T12134 | T12106 | punct | .,washed |
| R10078 | T12135 | T12136 | compound | Incubate,cells |
| R10079 | T12136 | T12136 | ROOT | cells,cells |
| R10080 | T12137 | T12136 | prep | with,cells |
| R10081 | T12138 | T12137 | pobj | PBS,with |
| R10082 | T12139 | T12140 | nummod | +1,% |
| R10083 | T12140 | T12141 | compound | %,BSA |
| R10084 | T12141 | T12136 | appos | BSA,cells |
| R10085 | T12142 | T12141 | prep | for,BSA |
| R10086 | T12143 | T12144 | nummod | 15,minutes |
| R10087 | T12144 | T12142 | pobj | minutes,for |
| R10088 | T12145 | T12146 | aux | to,block |
| R10089 | T12146 | T12136 | xcomp | block,cells |
| R10090 | T12147 | T12148 | amod | non-specific,binding |
| R10091 | T12148 | T12146 | dobj | binding,block |
| R10092 | T12149 | T12136 | punct | .,cells |
| R10093 | T12150 | T12151 | amod | Fixed,cells |
| R10094 | T12151 | T12154 | nsubjpass | cells,incubated |
| R10095 | T12152 | T12154 | auxpass | were,incubated |
| R10096 | T12153 | T12154 | advmod | then,incubated |
| R10097 | T12154 | T12154 | ROOT | incubated,incubated |
| R10098 | T12155 | T12154 | prep | with,incubated |
| R10099 | T12156 | T12155 | pobj | anti-FLAG,with |
| R10100 | T12157 | T12160 | punct | (,100 |
| R10101 | T12158 | T12160 | nummod | 1,100 |
| R10102 | T12159 | T12160 | nummod | ∶,100 |
| R10103 | T12160 | T12156 | appos | 100,anti-FLAG |
| R10104 | T12161 | T12160 | punct | ),100 |
| R10105 | T12162 | T12156 | prep | as,anti-FLAG |
| R10106 | T12163 | T12165 | det | the,antibody |
| R10107 | T12164 | T12165 | amod | primary,antibody |
| R10108 | T12165 | T12162 | pobj | antibody,as |
| R10109 | T12166 | T12165 | prep | at,antibody |
| R10110 | T12167 | T12168 | compound | room,temperature |
| R10111 | T12168 | T12166 | pobj | temperature,at |
| R10112 | T12169 | T12154 | prep | for,incubated |
| R10113 | T12170 | T12171 | nummod | 1,hour |
| R10114 | T12171 | T12169 | pobj | hour,for |
| R10115 | T12172 | T12171 | punct | ",",hour |
| R10116 | T12173 | T12154 | advcl | followed,incubated |
| R10117 | T12174 | T12173 | agent | by,followed |
| R10118 | T12175 | T12177 | nummod | three,washes |
| R10119 | T12176 | T12177 | amod | 5-minute,washes |
| R10120 | T12177 | T12174 | pobj | washes,by |
| R10121 | T12178 | T12154 | punct | .,incubated |
| R10122 | T12179 | T12182 | nsubjpass | Cells,incubated |
| R10123 | T12180 | T12182 | auxpass | were,incubated |
| R10124 | T12181 | T12182 | advmod | further,incubated |
| R10125 | T12182 | T12182 | ROOT | incubated,incubated |
| R10126 | T12183 | T12182 | prep | with,incubated |
| R10127 | T12184 | T12186 | amod | goat,IgG-FITC |
| R10128 | T12185 | T12186 | amod | anti-rabbit,IgG-FITC |
| R10129 | T12186 | T12183 | pobj | IgG-FITC,with |
| R10130 | T12187 | T12188 | punct | (,1 |
| R10131 | T12188 | T12190 | nummod | 1,100 |
| R10132 | T12189 | T12190 | nummod | ∶,100 |
| R10133 | T12190 | T12186 | appos | 100,IgG-FITC |
| R10134 | T12191 | T12186 | punct | ),IgG-FITC |
| R10135 | T12192 | T12182 | prep | as,incubated |
| R10136 | T12193 | T12195 | det | the,antibody |
| R10137 | T12194 | T12195 | amod | secondary,antibody |
| R10138 | T12195 | T12192 | pobj | antibody,as |
| R10139 | T12196 | T12195 | prep | at,antibody |
| R10140 | T12197 | T12198 | compound | room,temperature |
| R10141 | T12198 | T12196 | pobj | temperature,at |
| R10142 | T12199 | T12182 | prep | for,incubated |
| R10143 | T12200 | T12201 | nummod | 30,minutes |
| R10144 | T12201 | T12199 | pobj | minutes,for |
| R10145 | T12202 | T12182 | punct | ",",incubated |
| R10146 | T12203 | T12182 | advcl | followed,incubated |
| R10147 | T12204 | T12203 | agent | by,followed |
| R10148 | T12205 | T12207 | nummod | three,washes |
| R10149 | T12206 | T12207 | amod | 5-minute,washes |
| R10150 | T12207 | T12204 | pobj | washes,by |
| R10151 | T12208 | T12182 | punct | .,incubated |
| R10152 | T12209 | T12212 | nsubjpass | Cells,examined |
| R10153 | T12210 | T12212 | auxpass | were,examined |
| R10154 | T12211 | T12212 | advmod | then,examined |
| R10155 | T12212 | T12212 | ROOT | examined,examined |
| R10156 | T12213 | T12212 | advcl | using,examined |
| R10157 | T12214 | T12216 | amod | inverse,microscope |
| R10158 | T12215 | T12216 | compound | fluorescence,microscope |
| R10159 | T12216 | T12213 | dobj | microscope,using |
| R10160 | T12217 | T12212 | punct | .,examined |