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PubMed:16263187 JSONTXT

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Id Subject Object Predicate Lexical cue
T1 0-135 Title denotes Ability of Lactobacillus gasseri K 7 to inhibit Escherichia coli adhesion in vitro on Caco-2 cells and ex vivo on pigs' jejunal tissue.
T3 11-36 Bacteria denotes Lactobacillus gasseri K 7
T4 48-64 Bacteria denotes Escherichia coli
T5 86-98 Habitat denotes Caco-2 cells
T6 114-118 Habitat denotes pigs
T7 114-134 Habitat denotes pigs' jejunal tissue
T8 120-127 Habitat denotes jejunal
T2 136-1930 Paragraph denotes The ability of Lactobacillus (Lb.) gasseri K 7 to inhibit adhesion of Escherichia coli O8:K88 to intestinal mucosa was studied on cultured Caco-2 cells and ex vivo on pigs' small intestinal tissue. Lactobacilli were added simultaneously with E. coli, before E. coli and after E. coli for competition, exclusion and displacement assays. The concentration of lactobacilli on fully differentiated Caco-2 cells was 4.5+/-0.3 x 10(8) cfu/well, while the concentration of E. coli varied from 1.5 x 10(6) to 4.3 x 10(8) cfu/well. The number of E. coli adhered to Caco-2 monolayer (cfu/well) was lineary correlated (R(2)=0.97) to the concentration of added cells. In the assay simulating exclusion, E. coli adhesion was reduced by Lb. gasseri K 7 strain by 0.1 to 0.6 log cfu/well. The binding of E. coli was inhibited even more when incubated simultaneously with lactobacilli, particularly at the lowest concentration of E. coli (ratio E. coli/lactobacilli 1:248), where five-times reduction (or 0.7 log) was observed. When adhesion to tissue derived from pigs' jejunum was tested, concentration of E. coli was constant (6.9+/-0.14 x 10(7) cfu/ml), while the concentration of Lb. gasseri K 7 was 5.9 x 10(7) and 1.3 x 10(7) cfu/ml in two independent experiments, respectively. The adhesion of E. coli and Lb. gasseri K 7 cells to jejunal mucosa was similar (1.0+/-0.17 x 10(6) and 1.54+/-0.10 x 10(6) cfu/cm(2)) when the concentrations of single strains in suspensions were approximately the same. No significant competition, exclusion or displacement of E. coli by lactobacilli was observed on jejunal tissue. In conclusion, Lb. gasseri K 7 was found to be effective in reducing E. coli adhesion to Caco-2 enterocytes, but it was not able to do so in ex vivo conditions tested for pig jejunal tissue.
T9 151-182 Bacteria denotes Lactobacillus (Lb.) gasseri K 7
T10 206-229 Bacteria denotes Escherichia coli O8:K88
T11 233-243 Habitat denotes intestinal
T12 233-250 Habitat denotes intestinal mucosa
T13 266-287 Habitat denotes cultured Caco-2 cells
T14 303-307 Habitat denotes pigs
T15 303-332 Habitat denotes pigs' small intestinal tissue
T16 309-325 Habitat denotes small intestinal
T17 334-346 Bacteria denotes Lactobacilli
T18 378-385 Bacteria denotes E. coli
T19 394-401 Bacteria denotes E. coli
T20 412-419 Bacteria denotes E. coli
T21 493-505 Bacteria denotes lactobacilli
T22 509-542 Habitat denotes fully differentiated Caco-2 cells
T23 602-609 Bacteria denotes E. coli
T24 673-680 Bacteria denotes E. coli
T25 692-698 Habitat denotes Caco-2
T26 785-790 Habitat denotes cells
T27 827-834 Bacteria denotes E. coli
T28 859-874 Bacteria denotes Lb. gasseri K 7
T29 925-932 Bacteria denotes E. coli
T30 992-1004 Bacteria denotes lactobacilli
T31 1050-1057 Bacteria denotes E. coli
T32 1065-1072 Bacteria denotes E. coli
T33 1073-1085 Bacteria denotes lactobacilli
T34 1165-1171 Habitat denotes tissue
T36 1185-1189 Habitat denotes pigs
T35 1185-1198 Habitat denotes pigs' jejunum
T37 1228-1235 Bacteria denotes E. coli
T38 1305-1320 Bacteria denotes Lb. gasseri K 7
T39 1422-1429 Bacteria denotes E. coli
T40 1434-1449 Bacteria denotes Lb. gasseri K 7
T42 1459-1466 Habitat denotes jejunal
T41 1459-1473 Habitat denotes jejunal mucosa
T43 1684-1691 Bacteria denotes E. coli
T44 1695-1707 Bacteria denotes lactobacilli
T45 1724-1731 Habitat denotes jejunal
T46 1724-1738 Habitat denotes jejunal tissue
T47 1755-1770 Bacteria denotes Lb. gasseri K 7
T48 1809-1816 Bacteria denotes E. coli
T49 1829-1847 Habitat denotes Caco-2 enterocytes
T50 1911-1914 Habitat denotes pig
T51 1911-1929 Habitat denotes pig jejunal tissue
T52 1915-1922 Habitat denotes jejunal