UseCases_ArguminSci_Discourse  

PubMed:10526099 JSONTXT 10 Projects

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Id Subject Object Predicate Lexical cue
T1 0-146 DRI_Background denotes Early appearance of activated matrix metalloproteinase-9 and blood-brain barrier disruption in mice after focal cerebral ischemia and reperfusion.
T2 147-266 DRI_Outcome denotes Blood-brain barrier (BBB) disruption is thought to play a critical role in the pathophysiology of ischemia/reperfusion.
T3 267-424 DRI_Approach denotes Matrix metalloproteinases (MMPs) are a family of proteolytic enzymes that can degrade all the components of the extracellular matrix when they are activated.
T4 425-579 DRI_Approach denotes Gelatinase A (MMP-2) and gelatinase B (MMP-9) are able to digest the endothelial basal lamina, which plays a major role in maintaining BBB impermeability.
T5 580-717 DRI_Background denotes The present study examined the expression and activation of gelatinases before and after transient focal cerebral ischemia (FCI) in mice.
T6 718-783 DRI_Background denotes Adult male CD1 mice were subjected to 60 min FCI and reperfusion.
T7 784-938 DRI_Approach denotes Zymography was performed from 1 to 23 h after reperfusion using the protein extraction method with detergent extraction and affinity-support purification.
T8 939-1110 DRI_Background denotes MMP-9 expression was also examined by both immunohistochemistry and Western blot analysis, and tissue inhibitors to metalloproteinase-1 was measured by reverse zymography.
T9 1111-1180 DRI_Approach denotes The BBB opening was evaluated by the Evans blue extravasation method.
T10 1181-1308 DRI_Background denotes The 88-kDa activated MMP-9 was absent from the control specimens, while it appeared 3 h after transient ischemia by zymography.
T11 1309-1396 DRI_Approach denotes At this time point, the BBB permeability alteration was detected in the ischemic brain.
T12 1397-1518 DRI_Background denotes Both pro-MMP-9 (96 kDa) and pro-MMP-2 (72 kDa) were seen in the control specimens, and were markedly increased after FCI.
T13 1519-1621 DRI_Background denotes A significant induction of MMP-9 was confirmed by both immunohistochemistry and Western blot analysis.
T14 1622-1828 DRI_Challenge denotes The early appearance of activated MMP-9, associated with evidence of BBB permeability alteration, suggests that activation of MMP-9 contributes to the early formation of vasogenic edema after transient FCI.