PubMed:21861338 JSONTXT 2 Projects

[Anti-invasion effects of R- and S-enantiomers of equol on prostate cancer PC3, DU145 cells]. OBJECTIVE: To compare the effect of R- and S-enantiomers of equol on the motility and invasion in human prostate cancer cells PC3 and DU145. METHODS: Wound healing assay and transwell invasion assay were performed to assess the cell motility and invasion. Gelatin zymography and RT-PCR assay were performed to assess the related protein activity and mRNA expression. RESULT: R-(+) equol and S-(-)equol inhibited motility and invasion in PC3 and DU145 cells, separately. The results were more obvious in PC3 cells, and effect of R-(+) equol was stronger than its enantiomer S-(-) equol. MMP-2, MMP-9, the crucial members in metastasis, were found down-regulated by R-(+)equol and S-(-)equol. Subsequently, the estrogen receptor-alpha was activated by R-(+) equol, while no such effect on estrogen receptor-beta was observed. CONCLUSION: R-(+) equol and S-(-) equol inhibited motility and invasion in PC3 and DU145 cells, while the most strong effect was observed in PC3 cells by R-(+) equol, which might regulated by the activation of estrogen receptor-alpha.