| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
114-275 |
BACKGROUND |
denotes |
Despite the developmental impact of chromosome segregation errors, we lack the tools to assess environmental effects on the integrity of the germline in animals. |
| T2 |
288-403 |
OBJECTIVE |
denotes |
We developed an assay in Caenorhabditis elegans that fluorescently marks aneuploid embryos after chemical exposure. |
| T3 |
413-688 |
METHODS |
denotes |
We qualified the predictive value of the assay against chemotherapeutic agents as well as environmental compounds from the ToxCast Phase I library by comparing results from the C. elegans assay with the comprehensive mammalian in vivo end point data from the ToxRef database. |
| T4 |
698-929 |
RESULTS |
denotes |
The assay was highly predictive of mammalian reproductive toxicities, with a 69% maximum balanced accuracy. We confirmed the effect of select compounds on germline integrity by monitoring germline apoptosis and meiotic progression. |
| T5 |
943-1056 |
CONCLUSIONS |
denotes |
This C. elegans assay provides a comprehensive strategy for assessing environmental effects on germline function. |
