PubMed:12629989 JSONTXT 6 Projects

Expression of transforming growth factor-beta 1 in hepatocellular carcinoma in comparison with the non-tumor tissue. BACKGROUND/AIMS: TGF-beta 1 (transforming growth factor-beta 1) has been shown to be overexpressed in various cancer cells including hepatocellular carcinoma cells. In this study, we examined the association of the immunohistochemical expression of TGF-beta 1 in hepatocellular carcinoma tissues with the clinicopathological findings. METHODOLOGY: Thirty liver tumor biopsy specimens obtained with ultrasound guidance and in which tumor-tissue and non-tumor tissue existed were examined. TGF-beta 1 was stained using rabbit anti-human TGF-beta 1 antibody. RESULTS: TGF-beta 1 was expressed mainly in the cytoplasm and plasma membrane of the hepatocellular carcinoma cells, while it was stained in the fibrous septa and sinusoidal cells in the non-tumor tissue. When comparing the intensity of TGF-beta 1 staining in the hepatocellular carcinoma tissue with that in the non-tumor tissue, the former was more intense than the latter in 21 (70%) of the 30 cases. Although the expression of TGF-beta 1 in the hepatocellular carcinoma tissue was not related to the tumor size of hepatocellular carcinoma, it was correlated with the histological differentiation: the lower the histological differentiation grade of hepatocellular carcinoma was, the more intense was the TGF-beta 1 staining in hepatocellular carcinoma tissue. In addition, TGF-beta 1 tended to be overexpressed in the trabecular type of hepatocellular carcinoma. To elucidate the relationship of TGF-beta 1 staining with apoptosis in hepatocellular carcinoma cells, we performed terminal deoxynucleotidyl transferase-mediated DUTP biotinnick end labeling (TUNEL) in 11 of the 30 specimens. No positive cells for TUNEL were detected in the hepatocellular carcinoma tissues. CONCLUSIONS: These results indicated that the expression of TGF-beta 1 was associated with the histological differentiation of hepatocellular carcinoma cells.