PubMed_ArguminSci  

PubMed:30593373 JSONTXT 6 Projects

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Id Subject Object Predicate Lexical cue
T1 103-179 DRI_Background denotes In the present study, the mechanisms underlying Muscovy duck reovirus (MDRV)
T2 202-291 DRI_Background denotes ER stress and apoptosis in DF-1 cells and Muscovy duckling hepatic tissues were explored.
T3 292-542 DRI_Outcome denotes On the fifth day post-infection, an increase in the mRNA levels of binding immunoglobulin protein (Bip) and X-box binding protein (XBP1), activation of XBP1/s, and an increase in percentage of apoptotic cells were observed in Muscovy duckling livers.
T4 543-711 DRI_Background denotes The use of ER stress inducer Tunicamycin and ER stress inhibitor Tauroursodeoxycholic acid demonstrated that MDRV induces apoptosis via ER stress, leading to apoptosis.
T5 712-902 DRI_Background denotes The use of Tunicamycin increased viral protein synthesis while Tauroursodeoxycholic acid reduced viral protein synthesis, suggesting that MDRV induces ER stress benefiting virus replication.
T6 903-973 DRI_Approach denotes The MDRV p10.8 is the major protein to induce ER stress and apoptosis.
T7 974-1147 DRI_Approach denotes We found that p10.8 promotes the conversion of XBP1/u to XBP1/s and expands ER diameter, and increases the percentages of apoptotic cells in DF-1 and duckling liver tissues.
T8 1148-1314 DRI_Background denotes To investigate the mechanism underlying the MDRV p10.8-induced ER stress and apoptosis, Western blot, siRNA, and co-immunoprecipitation (Co-IP) assays were performed.
T9 1315-1407 DRI_Approach denotes We found that the MDRV p10.8 protein up-regulates Bip, p-IRE1, XBP1s, and cleaved-caspase 3.
T10 1658-1716 DRI_Background denotes Knockdown of XBP1 by siRNA reversed the increased level of
T11 1749-1751 DRI_Background denotes 3.
T12 1752-1934 DRI_Outcome denotes The present study provides mechanistic insights into the MDRV p10.8 protein induces ER stress, resulting in apoptosis via the Bip/IRE1/XBP1 pathway in DF-1 cells and duckling livers.