| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
160-417 |
DRI_Approach |
denotes |
To analyze differential gene expression of putative prostate tumor markers we compared the expression levels of more than 400 cancer-related genes using the cDNA array technique in a set of capsule-invasive prostate tumor and matched normal prostate tissue. |
| T2 |
418-579 |
DRI_Background |
denotes |
The overexpression of Bax inhibitor-1 (BI-1) in prostate carcinoma and prostate cancer cell lines was confirmed by using Northern blot and Western blot analyses. |
| T3 |
580-689 |
DRI_Background |
denotes |
Quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) on intact RNAs from 17 paired |
| T4 |
720-813 |
DRI_Background |
denotes |
epithelial tissue samples confirmed up-regulated BI-1 expression in 11 of 17 prostate tumors. |
| T5 |
814-966 |
DRI_Outcome |
denotes |
In addition, it was demonstrated that BI-1 expression is down-regulated in stromal cells as compared to matched normal epithelial cells of the prostate. |
| T6 |
967-1098 |
DRI_Background |
denotes |
In situ hybridization experiments on prostate sections also revealed that BI-1 expression is mainly restricted to epithelial cells. |
| T7 |
1099-1301 |
DRI_Background |
denotes |
Furthermore, quantitative RT-PCR on RNAs derived from five benign prostate hyperplasia (BPH) samples showed no significant difference in BI-1 expression as compared to normal epithelial prostate tissue. |
| T8 |
1302-1563 |
DRI_Background |
denotes |
To determine the function of BI-1 in vitro, human PC-3, LNCaP, and DU-145 prostate carcinoma cells were transfected with small interfering double-strand RNA (siRNA) oligonucleotides against the BI-1 gene leading to a specific down-regulation of BI-1 expression. |
| T9 |
1564-1731 |
DRI_Background |
denotes |
Furthermore, transfection of PC-3, LNCaP, and DU-145 cells with BI-1 sequence-specific siRNAs caused a significant increase in spontaneous apoptosis in all cell lines. |
| T10 |
1732-1949 |
DRI_Outcome |
denotes |
Taken together, our results indicate that the human BI-1 gene contains the potential to serve as a prostate cancer expression marker and as a potential target for developing therapeutic strategies for prostate cancer. |
